JP2701091B2 - 高粘度ポリマーマトリックスおよび調製方法および分離方法 - Google Patents
高粘度ポリマーマトリックスおよび調製方法および分離方法Info
- Publication number
- JP2701091B2 JP2701091B2 JP3504136A JP50413691A JP2701091B2 JP 2701091 B2 JP2701091 B2 JP 2701091B2 JP 3504136 A JP3504136 A JP 3504136A JP 50413691 A JP50413691 A JP 50413691A JP 2701091 B2 JP2701091 B2 JP 2701091B2
- Authority
- JP
- Japan
- Prior art keywords
- polymer
- matrix
- tube
- support matrix
- viscosity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
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- JRKICGRDRMAZLK-UHFFFAOYSA-L peroxydisulfate Chemical compound [O-]S(=O)(=O)OOS([O-])(=O)=O JRKICGRDRMAZLK-UHFFFAOYSA-L 0.000 description 1
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44795—Isoelectric focusing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44747—Composition of gel or of carrier mixture
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Dispersion Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Reinforced Plastic Materials (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Compositions Of Macromolecular Compounds (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
- Developing Agents For Electrophotography (AREA)
- Polymerisation Methods In General (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US472,045 | 1990-01-29 | ||
| US07/472,045 US5164055A (en) | 1990-01-29 | 1990-01-29 | High-viscosity polymer matrix and methods |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH06504612A JPH06504612A (ja) | 1994-05-26 |
| JP2701091B2 true JP2701091B2 (ja) | 1998-01-21 |
Family
ID=23873989
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP3504136A Expired - Lifetime JP2701091B2 (ja) | 1990-01-29 | 1991-01-25 | 高粘度ポリマーマトリックスおよび調製方法および分離方法 |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US5164055A (https=) |
| EP (1) | EP0513164B1 (https=) |
| JP (1) | JP2701091B2 (https=) |
| AT (1) | ATE178990T1 (https=) |
| DE (1) | DE69131124T2 (https=) |
| DK (1) | DK0513164T3 (https=) |
| ES (1) | ES2132084T3 (https=) |
| GR (1) | GR3030209T3 (https=) |
| WO (1) | WO1991011709A1 (https=) |
Families Citing this family (49)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5665216A (en) * | 1986-10-21 | 1997-09-09 | Northeastern University | Capillary column for high performance electrophoretic separation and detection of SDS proteins and system and using the same |
| US5332481A (en) * | 1991-01-29 | 1994-07-26 | Beckman Instruments, Inc. | Capillary electrophoresis using replaceable gels |
| US5126021A (en) * | 1991-07-17 | 1992-06-30 | Applied Biosystems Inc. | Low-viscosity polymer solution for capillary electrophoresis |
| US5213669A (en) * | 1992-01-31 | 1993-05-25 | Beckman Instruments, Inc. | Capillary column containing a dynamically cross-linked composition and method of use |
| EP0624248B1 (en) * | 1992-01-31 | 1998-04-15 | Beckman Instruments, Inc. | Capillary column containing removable separation gel composition and method of use |
| US5759369A (en) * | 1992-09-24 | 1998-06-02 | The Perkin-Elmer Corporation | Viscous electrophoresis polymer medium and method |
| US5290418A (en) * | 1992-09-24 | 1994-03-01 | Applied Biosystems, Inc. | Viscous electrophoresis polymer medium and method |
| JP3426236B2 (ja) * | 1992-11-05 | 2003-07-14 | ソーン バイオサイエンシス インコーポレイテッド | 電気泳動のための分離媒体 |
| US5569364A (en) * | 1992-11-05 | 1996-10-29 | Soane Biosciences, Inc. | Separation media for electrophoresis |
| US5891313A (en) * | 1993-11-23 | 1999-04-06 | The Perkin-Elmer Corp. | Entrapment of nucleic acid sequencing template in sample mixtures by entangled polymer networks |
| US6355709B1 (en) * | 1993-12-17 | 2002-03-12 | The Perkin-Elmer Corporation | Polymers for separation of biomolecules by capillary electrophoresis |
| AU1254295A (en) * | 1993-12-17 | 1995-07-03 | Perkin-Elmer Corporation, The | Uncharged polymers for separation of biomolecules by capillary electrophoresis |
| US6358385B1 (en) * | 1993-12-17 | 2002-03-19 | The Perkin-Elmer Corporation | Polymers for separation of biomolecules by capillary electrophoresis |
| US5423966A (en) * | 1994-01-25 | 1995-06-13 | Perkin-Elmer Corporation | On line ion contaminant removal apparatus and method for capillary electrophoresis |
| JP3727031B2 (ja) * | 1994-02-07 | 2005-12-14 | アプレラ コーポレイション | ポリヌクレオチド分析のための螢光に基づく電気泳動システム |
| US6485625B1 (en) | 1995-05-09 | 2002-11-26 | Curagen Corporation | Apparatus and method for the generation, separation, detection, and recognition of biopolymer fragments |
| US5582705A (en) * | 1995-05-19 | 1996-12-10 | Iowa State University Research Foundation, Inc. | Multiplexed capillary electrophoresis system |
| US5534123A (en) * | 1995-07-10 | 1996-07-09 | Molecular Dynamics | Denaturing separation matrix having hydroxyethyl cellulose for nucleic acid electrophoresis |
| US7244622B2 (en) * | 1996-04-03 | 2007-07-17 | Applera Corporation | Device and method for multiple analyte detection |
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-
1990
- 1990-01-29 US US07/472,045 patent/US5164055A/en not_active Expired - Lifetime
-
1991
- 1991-01-25 WO PCT/US1991/000545 patent/WO1991011709A1/en not_active Ceased
- 1991-01-25 AT AT91903988T patent/ATE178990T1/de not_active IP Right Cessation
- 1991-01-25 ES ES91903988T patent/ES2132084T3/es not_active Expired - Lifetime
- 1991-01-25 EP EP91903988A patent/EP0513164B1/en not_active Expired - Lifetime
- 1991-01-25 JP JP3504136A patent/JP2701091B2/ja not_active Expired - Lifetime
- 1991-01-25 DE DE69131124T patent/DE69131124T2/de not_active Expired - Lifetime
- 1991-01-25 DK DK91903988T patent/DK0513164T3/da active
-
1999
- 1999-05-13 GR GR990401294T patent/GR3030209T3/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP0513164A1 (en) | 1992-11-19 |
| EP0513164A4 (https=) | 1994-04-20 |
| DE69131124T2 (de) | 1999-09-23 |
| EP0513164B1 (en) | 1999-04-14 |
| GR3030209T3 (en) | 1999-08-31 |
| JPH06504612A (ja) | 1994-05-26 |
| WO1991011709A1 (en) | 1991-08-08 |
| DK0513164T3 (da) | 1999-10-25 |
| ATE178990T1 (de) | 1999-04-15 |
| ES2132084T3 (es) | 1999-08-16 |
| US5164055A (en) | 1992-11-17 |
| DE69131124D1 (de) | 1999-05-20 |
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