JP2618246B2 - Fluorescence measurement device - Google Patents

Description

The present invention relates to a fluorescence measuring device. In particular, the present invention relates to a fluorescence measurement device provided with a mechanism for preventing ultraviolet rays from leaking out of a housing when a measurement sample is exchanged.

b. Conventional technology When analyzing DNA (deoxyribonucleic acid) and the like, a method and an apparatus for irradiating ultraviolet rays to analyze an electrophoretic spectrum of a measurement sample and observing a fluorescence spectrum emitted from a substance excited by the ultraviolet rays have been developed. Are known.

c. Problems to be solved by the invention In the conventional fluorescence measurement apparatus, in order to avoid exposure to ultraviolet rays when exchanging the sample, the power supply of the ultraviolet light emission apparatus is manually shut off in advance to emit ultraviolet rays. Has been stopped and the sample has been replaced.

However, when the frequency of sample exchange is high, sometimes the power supply is not shut off.

SUMMARY OF THE INVENTION It is an object of the present invention to provide a fluorescence measurement device having a mechanism for automatically stopping the emission of ultraviolet light when a sample is replaced.

d. Means for solving the problem The above problem is caused by an ultraviolet light source provided in the housing,
A visible light cutoff filter provided on one side of the housing, and an ultraviolet absorption filter rotatably attached to the housing so as to be able to overlap the visible light cutoff filter; In a fluorescence measuring apparatus for measuring visible light emitted from a sample placed between the ultraviolet absorption filters through an ultraviolet absorption filter through a ultraviolet absorption filter, a switch operated by a rotational movement of the ultraviolet absorption filter; This problem has been solved by a fluorescence measurement device comprising: an irradiation stopping unit that stops irradiation of the visible light blocking filter with ultraviolet light when the filter is separated from a side surface provided with the visible light blocking filter.

e. Operation To place the sample on the visible light cutoff filter, turn the ultraviolet absorption filter open and turn on the switch.
It becomes F, and the irradiation of the ultraviolet light to the visible light cutoff filter is automatically stopped. After the sample is placed, the ultraviolet absorption filter is rotated and overlaid on the visible light cutoff filter, the sample is irradiated with ultraviolet light, and the fluorescence by the ultraviolet light is observed through the ultraviolet absorption filter.

f. Embodiment FIG. 1 is a partially cutaway perspective view of a preferred embodiment of the fluorescence measuring device according to the present invention, FIG. 2 is a conceptual cross-sectional elevation view of FIG. 1, and FIG. It is an example of an electric circuit used for a measuring device.

An ultraviolet ray generating tube 2 is housed in a housing 1. A glass filter, which is a visible light blocking filter 3, is provided on the upper surface of the housing, and only ultraviolet light is emitted outside the housing. Further, an ultraviolet absorbing filter 4 is mounted on the upper surface of the housing so as to be rotatable around a hinge 5. Since the visible light cutoff filter 3 and the ultraviolet absorption filter 4 are overlapped, there is no radiation of ultraviolet rays and visible light from the upper surface of the housing in principle.

However, as shown in FIG. 2, a fluorescent substance such as DN is provided between the visible light blocking filter 3 and the ultraviolet absorbing filter 4.
When the sample 6 for the electrophoresis spectrum observation of A is placed,
Only the fluorescence from the fluorescent substance in the sample is observed from outside the ultraviolet absorption filter 4.

When the ultraviolet absorption filter 4 is rotated for exchanging the sample 6, the micro switch 7 is turned off, the relay 8 linked with the micro switch 7 is operated, and the ultraviolet generation tube 2 is turned on.
The supply of power from the power supply 9 to the power supply is cut off, and the generation of ultraviolet rays stops. After replacing the sample, when the ultraviolet absorption filter 4 is put on the visible light cutoff filter 3 again, the micro switch 7 is turned on.
, Power is sent to the ultraviolet ray generating tube 2 via the relay 8 and the lamp is turned on.

FIG. 4 is a sectional view of another embodiment of the fluorescence measuring device according to the present invention, and FIG. 5 is an example of an electric circuit used in the fluorescence measuring device of FIG. FIG. 4 is a modification of FIG. 3, and the corresponding members are denoted by the same reference numerals and description thereof will be omitted.

A semi-cylindrical light-shielding tube 10 is provided so as to be rotatable around the ultraviolet ray generating tube 2. The light-shielding cylinder 10 is driven to rotate by a driving device 11 including, for example, a stepping motor.

As shown in FIG. 4, when the ultraviolet absorbing filter 4 is closed, the light shielding tube 10 occupies a position below the ultraviolet light generating tube 2, and the ultraviolet light is irradiated upward through the opening 10a of the light shielding tube. When the ultraviolet absorbing filter 4 is opened, the micro switch 7 is turned off, and the light shielding cylinder 10
゜ Rotate. As a result, a light shielding tube is provided above the ultraviolet ray generating tube 2.
The ultraviolet rays are blocked by the light-shielding tube 10, the partition 11, and the housing 1. In this case, since the ultraviolet ray generating tube 2 is always lit, it is easy to maintain the ultraviolet intensity stably, and the life of the ultraviolet ray generating tube 2 can be extended. Conventional technology can be used for the cooling fan and the like. Therefore, they are omitted in the drawings.

g. Effects of the Invention When the ultraviolet absorption filter is opened and the sample is changed, the irradiation of the ultraviolet light is automatically stopped without fail.

[Brief description of the drawings]

FIG. 1 is a partially cutaway perspective view of a preferred embodiment of a fluorescence measuring device according to the present invention, FIG. 2 is a conceptual sectional elevation view of FIG.
FIG. 3 is an electric circuit used in the fluorescence measuring device of FIG. 1,
FIG. 4 is a sectional view of another embodiment of the fluorescence measuring device according to the present invention, and FIG. 5 is an electric circuit used in the fluorescence measuring device of FIG. DESCRIPTION OF SYMBOLS 1 ... Housing, 2 ... UV generating tube, 3 ... Visible light cutoff filter, 4 ... UV absorbing filter, 5 ... Hinge, 6 ... Sample, 7 ... Micro switch, 8 ... Relay, 9 ... ... power supply, 10 ... light shield tube, 11 ... drive device,

Claims (3)

(57) [Claims] An ultraviolet light emitting source provided in a housing, a visible light blocking filter provided on one side of the housing, and a rotatable housing so as to be able to be superimposed on the visible light blocking filter. With an attached ultraviolet absorbing filter,
In a fluorescence measuring device in which a sample placed between the visible light cutoff filter and the ultraviolet absorption filter measures visible light emitted by ultraviolet light through the ultraviolet absorption filter, the sample is operated by the rotational movement of the ultraviolet absorption filter. A fluorescence measurement device comprising: a switch; and an irradiation stopping unit that stops irradiation of the visible light blocking filter with ultraviolet light when the ultraviolet absorption filter is separated from a side surface provided with the visible light blocking filter. . 2. The apparatus according to claim 1, wherein said irradiation stopping means is a relay provided in front of a power supply of the ultraviolet light emitting source, and stops the ultraviolet light emission by shutting off the power supply. Fluorescence measurement device. 3. The fluorescence measuring apparatus according to claim 1, wherein said fluorescence measuring apparatus is an apparatus for observing an electrophoretic spectrum.