JP2615594B2 - Concentrator - Google Patents
ConcentratorInfo
- Publication number
- JP2615594B2 JP2615594B2 JP62044462A JP4446287A JP2615594B2 JP 2615594 B2 JP2615594 B2 JP 2615594B2 JP 62044462 A JP62044462 A JP 62044462A JP 4446287 A JP4446287 A JP 4446287A JP 2615594 B2 JP2615594 B2 JP 2615594B2
- Authority
- JP
- Japan
- Prior art keywords
- sample
- section
- electrophoresis
- detector
- flow path
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Peptides Or Proteins (AREA)
Description
【発明の詳細な説明】 （産業上の利用分野） 本発明は溶媒中に例えば蛋白質などの試料が懸濁した
溶液を濃縮する装置に関するものである。Description: TECHNICAL FIELD The present invention relates to an apparatus for concentrating a solution in which a sample such as a protein is suspended in a solvent.
（従来の技術） 生物工学の分野で処理された蛋白質などの試料は、多
量の溶媒中に懸濁していることが多く、工業的に利用し
ようとすれば試料を濃縮する必要がある。(Prior Art) A sample such as a protein processed in the field of biotechnology is often suspended in a large amount of a solvent, and it is necessary to concentrate the sample for industrial use.
（発明が解決しようとする問題点） 蛋白質などの試料を効率的に濃縮する装置は現在のと
ころ知られていない。(Problems to be Solved by the Invention) An apparatus for efficiently concentrating a sample such as a protein is not known at present.
本発明は電気泳動手法を用いて多量の溶媒中の試料を
濃縮する装置を提供することを目的とするものである。An object of the present invention is to provide an apparatus for concentrating a sample in a large amount of a solvent using an electrophoresis technique.
（問題点を解決するための手段） 本発明の濃縮装置は、円盤状の泳動部と、その泳動部
の外周部を取り囲み、泳動部の外周につがなってリング
状に設けられ、試料導入口をもつ試料充填部と、その泳
動部の中心部で泳動部の内周につながり、取出し口をも
つ試料取出し部と、試料導入口に設けられた送液ポンプ
と、取出し口に設けられ、試料の濃度を測定する検出器
と、送液ポンプと検出器の間を流路切換手段を介して接
続した試料の供給、循環及び取出し用の流路と、泳動部
で試料を試料充填部から試料取出し部方向に電気泳動さ
せる電源を供給する電源装置と、検出器の検出信号によ
り流路切換手段の切換え動作を制御する駆動回路と、を
備えている。(Means for Solving the Problems) The concentrating apparatus of the present invention surrounds a disk-shaped electrophoresis section and an outer periphery of the electrophoresis section, is connected to the outer circumference of the electrophoresis section, and is provided in a ring shape. A sample filling section having a sample outlet connected to the inner periphery of the electrophoresis section at the center of the electrophoresis section and having an outlet, a liquid feed pump provided at the sample inlet, and a sample A detector for measuring the concentration of the sample, a flow path for supplying, circulating and taking out the sample which is connected between the liquid sending pump and the detector via flow path switching means, The power supply device includes a power supply device that supplies power for performing electrophoresis in the direction of the takeout unit, and a drive circuit that controls a switching operation of the flow path switching unit based on a detection signal of the detector.
（実施例） 第１図は一実施例を表わし、第２図は第１図のＡ−Ａ
線位置での断面図を表わす。(Embodiment) FIG. 1 shows one embodiment, and FIG. 2 shows AA of FIG.
FIG. 3 shows a cross-sectional view at the line position.
２は泳動槽であり、２枚の絶縁物製円板4,6が対向
し、その対向した隙間部分が泳動部８となっている。泳
動部８の隙間は1mm程度えだり、泳動部８の半径は40cm
程度である。泳動槽２の外周部と中心部はともに閉じら
れており、外周部には泳動槽８の外周につながる試料充
填部10がリング状に設けられ、中心部では泳動部８の内
周につながる試料取出し部12がリング状に設けられてい
る。試料充填部10には試料導入口14が設けられ、試料取
出し部12には取出し口16が設けられている。Reference numeral 2 denotes an electrophoresis tank, and two insulating discs 4 and 6 are opposed to each other, and a gap between the opposed discs is an electrophoresis section 8. The gap of the migration part 8 is about 1 mm, and the radius of the migration part 8 is 40 cm.
It is about. The outer peripheral part and the central part of the electrophoresis tank 2 are both closed, and a sample filling part 10 connected to the outer peripheral part of the electrophoretic tank 8 is provided in a ring shape at the outer peripheral part. The take-out part 12 is provided in a ring shape. The sample filling section 10 is provided with a sample introduction port 14, and the sample removal section 12 is provided with a removal port 16.
試料充填部10と試料取出し部12にはそれぞれ電極18,2
0が設けられており、試料充填部の電極18が陰極、試料
取出し部の電極20が陽極となるように電源が供給され
る。Electrodes 18 and 2 are provided in sample loading section 10 and sample removal section 12, respectively.
0 is provided, and power is supplied so that the electrode 18 of the sample filling section serves as a cathode and the electrode 20 of the sample removing section serves as an anode.
試料導入口14には試料溶液を供給するポンプ22が設け
られており、ポンプ22よりも上流側には三方切換えバル
ブ24が設けられている。三方切換えバルブ24の一方の出
入口は試料瓶26に接続され、他方の出入口は取出し口16
につながる三方切換えバルブ28に接続されている。The sample inlet 14 is provided with a pump 22 for supplying a sample solution, and a three-way switching valve 24 is provided upstream of the pump 22. One port of the three-way switching valve 24 is connected to the sample bottle 26, and the other port is
Is connected to a three-way switching valve 28 which leads to
取出し口16には試料の濃度を測定する検出器30が設け
られており、検出器30よりも下流側に三方切換えバルブ
28が設けられている。三方切換えバルブ28の一方の出入
口は前記の試料供給側の三方切換えバルブ24に接続され
ており、他方の出入口は濃縮された試料溶液を収容する
トラップ32に接続されている。The outlet 16 is provided with a detector 30 for measuring the concentration of the sample, and a three-way switching valve is provided downstream of the detector 30.
28 are provided. One port of the three-way switching valve 28 is connected to the three-way switching valve 24 on the sample supply side, and the other port is connected to a trap 32 containing a concentrated sample solution.
検出器30の検出信号は駆動回路34に入力され、三方切
換えバルブ24,28は駆動回路34によって切換え動作が制
御される。The detection signal of the detector 30 is input to the drive circuit 34, and the switching operation of the three-way switching valves 24 and 28 is controlled by the drive circuit 34.
次に、本実施例の動作について説明する。 Next, the operation of the present embodiment will be described.
試料瓶26には多量の溶媒中に例ば遺伝子組替え操作が
施こされたバイオ産物などの試料が懸濁した試料溶液が
収納されている。まず、三方切換えバルブ24を試料瓶26
側に切り換え、三方切換えバルブ28を三方切換えバルブ
24側に切り換えておく。ポンプ22を動作させて試料瓶26
の試料を試料充填部10に供給する。そして、ポンプ22を
止め、三方切換えバルブ24を三方切換えバルブ28側に切
り換えておく。The sample bottle 26 contains a sample solution in which a sample such as a bioproduct subjected to a genetic recombination operation is suspended in a large amount of solvent. First, the three-way switching valve 24 is connected to the sample bottle 26.
Side, and the three-way switching valve 28 is replaced with a three-way switching valve
Switch to 24. Operate the pump 22 to operate the sample bottle 26
Is supplied to the sample filling section 10. Then, the pump 22 is stopped, and the three-way switching valve 24 is switched to the three-way switching valve 28 side.
電極18,20の間に電圧を印加して試料の電気泳動を起
こさせる。試料は電気泳動によって試料充填部10から試
料取出し部12の方向に向って泳動する。このとき、泳動
部８は外周部では保持容量が大きく中心部では保持容量
が小さくなっているので、試料が中心部に向って泳動さ
れるに従って濃縮されていく。A voltage is applied between the electrodes 18 and 20 to cause electrophoresis of the sample. The sample migrates from the sample filling unit 10 to the sample removal unit 12 by electrophoresis. At this time, since the holding capacity of the electrophoresis section 8 is large at the outer periphery and small at the center, the sample is concentrated as the sample is migrated toward the center.
濃縮された試料は試料取出し部12から取出し口16を通
って三方切換えバルブ28,24を経て再び試料充填部10に
供給され、電気泳動が繰り返されて更に濃縮が行なわれ
る。The concentrated sample is supplied from the sample extracting section 12 to the sample filling section 10 again through the outlet 16 via the three-way switching valves 28 and 24, and the electrophoresis is repeated to further concentrate.
検出器30は取出し口16から出る試料の濃度を連続して
モニターしている。Detector 30 continuously monitors the concentration of the sample exiting outlet 16.
検出部30が試料濃度が目的の濃度になったことを検出
すると、駆動回路34によて三方切換えバルブ28がトラッ
プ32側に切り換えられて濃縮された試料がトラップ32に
分取される。When the detection unit 30 detects that the sample concentration has reached the target concentration, the drive circuit 34 switches the three-way switching valve 28 to the trap 32 side, and the concentrated sample is collected in the trap 32.
その後、三方切換えバルブ24が試料瓶26側に切り換え
られ、三方切換えバルブ28が再び三方切換えバルブ24側
に切り換えられて、試料瓶26から新たに試料が試料充填
部10に供給され、上記の濃縮動作が繰り返えされる。Thereafter, the three-way switching valve 24 is switched to the sample bottle 26 side, the three-way switching valve 28 is again switched to the three-way switching valve 24 side, and a new sample is supplied from the sample bottle 26 to the sample filling unit 10, and the above-described concentration is performed. The operation is repeated.
上記の実施例では駆動回路34によって検出器30の検出
信号にしたがって三方切換えバルブ24,28を操作するこ
とにより多量の溶媒中に懸濁した試料を連続的に濃縮し
て分取することができる。In the above embodiment, the sample suspended in a large amount of solvent can be continuously concentrated and collected by operating the three-way switching valves 24 and 28 according to the detection signal of the detector 30 by the drive circuit 34. .
第１図には泳動槽２が１セットだけ示されているが、
泳動槽２を多段に積み重ね、全ての泳動槽について電極
18,20を共通に使用するようにしてもよい。Although only one set of the electrophoresis tank 2 is shown in FIG.
Electrophoresis tanks 2 are stacked in multiple stages, and electrodes are
18, 20 may be commonly used.
第１図及び第２図の実施例では泳動部８を単なる隙間
としているが、泳動部８にポリアクリルアミドなどのゲ
ルを充填することができる。ゲルを充填した場合には泳
動部８の厚さを例えば1cm程度まで厚くすることができ
る。Although the electrophoresis section 8 is merely a gap in the embodiment shown in FIGS. 1 and 2, the electrophoresis section 8 can be filled with a gel such as polyacrylamide. When the gel is filled, the thickness of the electrophoresis section 8 can be increased to, for example, about 1 cm.
（発明の効果） 本発明の濃縮装置では、試料充填部が泳動部の外周部
を取り囲んでいるので、一度に大量の試料を処理するこ
とができる。(Effect of the Invention) In the concentration device of the present invention, since the sample filling section surrounds the outer periphery of the electrophoresis section, a large amount of sample can be processed at one time.
また、泳動部の試料導入口と取出し口の間を循環用の
流路で接続しているので、濃縮動作を自動的に繰り返す
ことができ、しかもその流路には試料の濃度を測定する
検出器を設けて試料の濃度をモニタするので、所定の濃
度まで濃縮された時点で流路切換手段により流路を切り
換えて所定の濃縮度をもつ試料液を取り出すことができ
る。In addition, since the sample inlet and outlet of the electrophoresis section are connected by a circulation channel, the enrichment operation can be repeated automatically, and the flow channel detects the concentration of the sample. Since the concentration of the sample is monitored by providing a vessel, the flow path is switched by the flow path switching means at the time when the concentration of the sample is concentrated to the predetermined concentration, and the sample liquid having the predetermined concentration can be taken out.
第１図は一実施例を示す概略斜視図、第２図は第１図の
Ａ−Ａ線位置での断面図である。 ２……泳動槽、 ８……泳動部、 10……試料充填部、 12……試料取出し部、 14……試料導入口、 16……取出し口、 18,20……電極。FIG. 1 is a schematic perspective view showing one embodiment, and FIG. 2 is a sectional view taken along a line AA in FIG. 2 ... electrophoresis tank, 8 ... electrophoresis section, 10 ... sample filling section, 12 ... sample extraction section, 14 ... sample introduction port, 16 ... extraction port, 18, 20 ... electrode.
Claims (1)
ってリング状に設けられ、試料導入口をもつ試料充填部
と、 その泳動部の中心部で泳動部の内周につながり、取出し
口をもつ試料取出し部と、 前記試料導入口に設けられた送液ポンプと、 前記取出し口に設けられ、試料の濃度を測定する検出器
と、 前記送液ポンプと検出器の間を流路切換手段を介して接
続した試料の供給、循環及び取出し用の流路と、 泳動部で試料を試料充填部から試料取出し部方向に電気
泳動させる電源を供給する電源装置と、 前記検出器の検出信号により前記流路切換手段の切換え
動作を制御する駆動回路と、を備えたことを特徴とする
濃縮装置。1. A disk-shaped electrophoresis section, a sample filling section surrounding the outer circumference of the electrophoresis section, connected to the outer circumference of the electrophoresis section and provided in a ring shape and having a sample introduction port, and a central portion of the electrophoresis section. A sample take-out part connected to the inner periphery of the electrophoresis part and having a take-out port; a liquid feed pump provided at the sample inlet; a detector provided at the take-out port to measure the concentration of the sample; A flow path for supplying, circulating, and taking out the sample, which is connected between the liquid pump and the detector via the flow path switching means, and a power supply that causes the electrophoresis section to electrophorese the sample from the sample filling section to the sample taking out section. And a drive circuit for controlling a switching operation of the flow path switching means based on a detection signal of the detector.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62044462A JP2615594B2 (en) | 1987-02-25 | 1987-02-25 | Concentrator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62044462A JP2615594B2 (en) | 1987-02-25 | 1987-02-25 | Concentrator |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS63208752A JPS63208752A (en) | 1988-08-30 |
| JP2615594B2 true JP2615594B2 (en) | 1997-05-28 |
Family
ID=12692159
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62044462A Expired - Fee Related JP2615594B2 (en) | 1987-02-25 | 1987-02-25 | Concentrator |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2615594B2 (en) |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS613041A (en) * | 1984-06-18 | 1986-01-09 | Hitachi Ltd | Apparatus for determining base sequence of nucleic acid |
-
1987
- 1987-02-25 JP JP62044462A patent/JP2615594B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPS63208752A (en) | 1988-08-30 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| LAPS | Cancellation because of no payment of annual fees |