JP2571702B2 - Prevention and treatment of constipation for pigs - Google Patents

Description

The present invention relates to an agent for preventing and treating constipation for pigs.

[Conventional technology]

Herbivore animals such as pigs rarely cause constipation due to their daily intake of high fiber feed. However, significant constipation occurs frequently in sows during the period from the second trimester to immediately after delivery. And sow constipation often causes fever, leading to reduced lactation. Therefore, preventing or treating sow constipation and maintaining good health in breeding pigs will speed up postpartum sow recovery as well as reduce calving and reduce pre- and postpartum accidents. It is important to accelerate the growth of piglets.

Conventionally, for prevention or treatment of constipation in pigs, treatments such as injection of constipation drugs and enema have been performed. However, injection of constipation drugs has side effects such as diarrhea and loss of appetite, and also has the problem of causing undesirable stress on nervous pregnancy (or immediately after delivery) dams. Enemas also have the disadvantage that they are not only temporarily effective, but also take time to implement. In addition, as a preventive method, there is a method in which bran, barley, or the like is added to a feed in a large amount, and the feed is continuously administered. However, there is a problem that the feed cost increases.

[Problems to be Solved by the Invention] An object of the present invention is to provide means for preventing and treating constipation for pigs, which does not have the drawbacks of the above-mentioned conventional methods.

[Means for solving the problem]

The present invention relates to a drug different from any of the conventional agents for preventing or treating constipation in pigs, namely, a general formula Gal- (Gal) n-Glc (where Gal is a galactose residue, Glc is a glucose residue, and n is 1 The present invention provides an agent for preventing and treating constipation in pigs, which comprises an oligosaccharide represented by an integer of 1 to 4 (hereinafter, referred to as galacto-oligosaccharide) as an active ingredient.

Galactooligosaccharides are a major component of breast milk oligosaccharides and are known to be useful as growth-promoting factors for effective bacteria, Bifidobacterium, which inhabit the human intestine. Therefore, in recent years, it has been used in various fields, such as being added to fermented milk and infant milk powder. Japanese Patent Application Laid-Open No. 62-138147 discloses that this oligosaccharide is used for preventing diarrhea and loose stool and increasing weight of livestock. However, galacto-oligosaccharides have never been known to be effective in preventing and treating constipation in pigs.

The galacto-oligosaccharide, which is an active ingredient of the preventive / therapeutic agent for constipation for swine of the present invention, is prepared by a method of allowing β-galactosidase of Aspergillus oryzae to act on lactose (Japanese Patent Publication No. 58-20)
No. 266) and a method using a yeast belonging to the genus Cryptococcus (Japanese Patent Application Laid-Open No. 60-251896). According to these production methods, usually, a solution of a sugar mixture containing unreacted lactose, by-produced transfer disaccharide, glucose, galactose and the like in addition to about 20 to 60% of galactooligosaccharide is obtained. The agent for preventing or treating constipation of the pig of the present invention can be used as it is, but may be further reduced with the content of unreacted lactose by the action of β-galactosidase of different origin. It is needless to say that a purified product of galacto-oligosaccharide having a higher purity may be used.

In order to use galactooligosaccharide as an agent for preventing or treating constipation for pigs, the sugar mixture or the purified product obtained as described above is orally administered as it is, or is mixed with feed or drinking water and administered. Galactooligosaccharides have a weak sweetness and are well soluble in water, so that swine will not be repelled when administered in feed or drinking water.

The dosage is about 0.02-1.0 g / kg per day, especially 0.06-
0.4g / kg is appropriate.

〔Example〕

 Hereinafter, the present invention will be described with reference to examples.

Example 1 4 kg of food-grade lactose was dissolved in 2.4 hot water, 80,000 units of β-galactosidase of Aspergillus oryzae were added, and reacted at 67 ° C for 2 hours. Thereafter, the reaction solution is heated to 95 ° C.
After heating for 10 minutes to inactivate the enzyme and diluting to a solid concentration of 50%, add 5,000 units of β-galactosidase prepared from Streptococcus thermophilus, and add 45 ° C.
For 40 hours. Thereafter, the reaction solution was heated to 90 ° C. for 10 minutes to inactivate the enzyme, powdered charcoal and celite were added, the mixture was filtered, and the filtrate was desalted using an ion exchange resin.

The obtained sugar solution was subjected to the following test using a concentrated solution (galacto-oligosaccharide 27.8%, disaccharide 21.1%, monosaccharide 26.1%, water 25%) as an agent for preventing and treating constipation for pigs.

Test Example 1 Among 60 sows from 50 days of gestation to 10 days after parturition, 20 animals with constipation were divided into two groups. 1) a day
25 mg was injected for 2 days. To 10 mice in group B, 40 ml of the sugar solution according to Example 1 was sprinkled once a day for 3 days on the combined feed.

 The treatment progress of both groups is shown in Table 1 (the numerical value is the number of heads).

Example 2 4 kg of food-grade lactose was dissolved in hot water of 2.4, 80,000 units of β-galactosidase of Aspergillus oryzae were added, and the mixture was reacted at 67 ° C for 2 hours. Thereafter, the reaction solution is heated to 95 ° C.
For 10 minutes to inactivate the enzyme, added activated carbon and celite, filtered and concentrated to 75% solids.

The resulting sugar solution (galactooligosaccharide 22.5%, disaccharide 33.7
%, Monosaccharides 18.8%) were subjected to the following tests as an agent for preventing and treating constipation for pigs.

Test Example 2 200 breeding mother pigs were divided into three groups, and group A was fed a normal complete feed mixture. For group B, the above feed was sprinkled with 10 ml of the sugar solution of Example 2 once a day. Group C was fed with the above feed supplemented with 20% of ground barley.

The percentage of pigs that became constipated during breeding under the above conditions was group A,
Group B and Group C were 31.4%, 6.6% and 11.7%, respectively.

〔The invention's effect〕

As is clear from the results of the test examples, according to the constipation preventive / therapeutic agent for pigs of the present invention, constipation can be prevented and administered without giving any stress to pigs by a simple administration method of sprinkling on feed. Can be treated.

A particular advantage of the constipation preventive / therapeutic agent of the present invention is that it is a saccharide as an active ingredient and has no undesirable side effects or preference inhibition and can be administered continuously.

──────────────────────────────────────────────────の Continuation of the front page (51) Int.Cl. ⁶ Identification code Agency reference number FI Technical display location C12P 19/14 C12P 19/14 Z (72) Inventor Tetsuo Terashima 1-Higashi-Shimbashi, Minato-ku, Tokyo 1-19 Yakult Honsha Co., Ltd. (72) Inventor Yoji Onari 1624 Okazaki, Hiratsuka-shi, Kanagawa Pref. Hirai Livestock Industry Co., Ltd. (56) References JP-A-63-63366 (JP, A)

Claims (1)

(57) [Claims] (1) The general formula Gal- (Gal) n-Glc (wherein G
al is a galactose residue, Glc is a glucose residue, n is 1
A constipation preventive / therapeutic agent for pigs, comprising an oligosaccharide represented by the following formulas (1 to 4 each representing an integer):