JP2553955B2 - Method for sterilizing petri dishes made of synthetic resin for culture medium - Google Patents
Method for sterilizing petri dishes made of synthetic resin for culture mediumInfo
- Publication number
- JP2553955B2 JP2553955B2 JP2208011A JP20801190A JP2553955B2 JP 2553955 B2 JP2553955 B2 JP 2553955B2 JP 2208011 A JP2208011 A JP 2208011A JP 20801190 A JP20801190 A JP 20801190A JP 2553955 B2 JP2553955 B2 JP 2553955B2
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- JP
- Japan
- Prior art keywords
- petri dish
- medium
- synthetic resin
- electron beam
- blood agar
- Prior art date
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Description
【発明の詳細な説明】 （産業上の利用分野） 本発明は血液寒天培地、組織培地又は合成培地等の培
地用合成樹脂製シャーレの滅菌方法に関するものであ
る。TECHNICAL FIELD The present invention relates to a method for sterilizing a petri dish made of a synthetic resin for a medium such as a blood agar medium, a tissue medium or a synthetic medium.
（従来の技術及びその課題） 培地用合成樹脂製シャーレに関する従来の滅菌方法と
してはエチレンオキサイドガス（以下「EOG」と称す
る）によるEOG滅菌や、放射線の一種であるＹ線による
Ｙ線滅菌等がある。(Prior Art and Its Problems) Conventional sterilization methods for synthetic resin petri dishes for culture media include EOG sterilization with ethylene oxide gas (hereinafter referred to as “EOG”) and Y-ray sterilization with Y-rays, which is a type of radiation. is there.
培地に関して、殊に血液寒天培地は、寒天培地中に血
液を添加・混合することにより調製されるものであり、
これはシャーレに分注され、凝固させて保管される。こ
の血液寒天培地は、培地中に懸濁された赤血球が細菌の
培養時に溶血を生じるか否かを調べるために使用され、
従って、細菌培養に先立つ保管中に赤血球が溶血し或い
は培地が変色したのでは血液寒天培地としての価値が損
われてしまう。Regarding the medium, in particular the blood agar medium is prepared by adding and mixing blood in the agar medium,
This is dispensed into a petri dish, solidified and stored. This blood agar medium is used to investigate whether red blood cells suspended in the medium cause hemolysis during bacterial culture,
Therefore, if red blood cells are hemolyzed or the medium is discolored during storage prior to bacterial culture, the value as a blood agar medium will be impaired.
従来のEOG滅菌は、滅菌用ガスがシャーレ中に僅かな
りとも残留することがある点及び処理後において微生物
等の残存する場合がある点に課題があり、しかも血液寒
天培地に用いるシャーレを対象とする場合には培地の経
時的溶血・変色等の劣化が生じると共に、培地の経時的
発育支持能低下を招く点に課題があった。Conventional EOG sterilization has a problem in that the sterilizing gas may remain in the petri dish even slightly, and microorganisms may remain after the treatment, and the petri dish used for blood agar medium is targeted. In this case, there is a problem in that deterioration of the medium such as hemolysis and discoloration with time occurs, and that the growth supporting ability of the medium deteriorates with time.
一方、Ｙ線滅菌はシャーレ自体の物性面に与える影響
が大きくて着色を招き、線源の管理・維持が面倒であ
り、滅菌に要する時間が長く、シールドの必要性があ
り、更にコスト高である点に課題がある。On the other hand, Y-ray sterilization has a large effect on the physical properties of the petri dish itself, which causes coloration, which makes management and maintenance of the radiation source troublesome, requires a long time for sterilization, requires a shield, and is more expensive. There is a problem in one point.
本発明は従来技術における上記の課題を解消するため
になされたものであり、その目的は確実に滅菌でき、培
地の経時的変化及び経時的発育支持能低下を防止乃至抑
制すると共に、低コストで実施し得る、培地用合成樹脂
製シャーレの滅菌方法を提供することにある。The present invention has been made to solve the above problems in the prior art, the purpose can be reliably sterilized, to prevent or suppress the change over time of the medium and the decrease in growth support capacity over time, at low cost. It is to provide a method of sterilizing a synthetic resin petri dish for culture that can be carried out.
（課題を解決するための手段及び作用） 本発明によれば、包装容器内に積み重ねられ且つ所定
数収納された培地用合成樹脂製シャーレに、包装容器の
外部から電子線を実質的吸収線量で10kGy以上照射する
ことを特徴とする、培地用合成樹脂製シャーレの滅菌方
法により、上記の課題が解決されると共に、上記の目的
が達成される。(Means and Actions for Solving the Problems) According to the present invention, a synthetic resin petri dish for culture medium stacked in a packaging container and stored in a predetermined number, an electron beam from the outside of the packaging container with a substantially absorbed dose. A method for sterilizing a petri dish made of a synthetic resin for a medium, which is characterized by irradiating at least 10 kGy, solves the above problems and achieves the above objects.
即ち、本発明方法を利用することにより合成樹脂製シ
ャーレに付着している雑菌、微生物等が極めて強力な電
子線の照射により短時間で死滅し、照射された電子線が
残留せず且つ照射処理が合成樹脂製シャーレ自体の物性
面に与える影響が少ないのである。That is, by using the method of the present invention, bacteria and microorganisms attached to the synthetic resin petri dish are killed in a short time by the irradiation of an extremely strong electron beam, and the irradiated electron beam does not remain and the irradiation treatment is performed. Has little influence on the physical properties of the synthetic resin petri dish itself.
本発明において用いる培地用合成樹脂製シャーレの素
材としては透明性を有する合成樹脂であれば格別の限定
はなく、例えばポリスチレン、AS樹脂、ABS樹脂、ポリ
エチレン、ポリプロピレン、アクリル樹脂、ポリエチレ
ンテレフタレート、ポリカーボネート、メチルペンテン
ポリマー、ポリスルホン、ポリアミド、ポリアセター
ル、ポリエステル、塩化ビニル樹脂等であることができ
る。The material of the synthetic resin petri dish for medium used in the present invention is not particularly limited as long as it is a synthetic resin having transparency, for example, polystyrene, AS resin, ABS resin, polyethylene, polypropylene, acrylic resin, polyethylene terephthalate, polycarbonate, It can be a methylpentene polymer, polysulfone, polyamide, polyacetal, polyester, vinyl chloride resin and the like.
これらの合成樹脂製シャーレは、EOG滅菌の場合と同
様に、JIS規格の丸シャーレ（本体部の直径90mm、高さ:
15mm、壁厚:0.8−1.0mm）であれば、通例20組（１組：
本体部＋蓋体）を積み重ね、これを縦・横５列宛、即ち
合計500組がポリエチレン製の袋に収められ、これがダ
ンボール箱等の包装容器内に収納され、当該容器が封緘
された荷姿状態で滅菌処理に付される。As with EOG sterilization, these synthetic resin petri dishes are JIS standard round petri dishes (body diameter 90 mm, height:
If it is 15mm, wall thickness: 0.8-1.0mm, 20 pairs (1 pair:
(Main body + lid) are stacked, and these are addressed in vertical and horizontal 5 rows, that is, a total of 500 sets are stored in a polyethylene bag, which is stored in a packaging container such as a cardboard box, and the container is sealed. Sterilized as it is.
本発明における電子線の照射は電子線加速装置を用い
て行われる。該電子線加速装置としては、高エネルギー
の電子線を発するものであれば特に限定されず、例えば
住友重機械工業株式会社製の「ダイナミトロン（標
章）」が使用される。該電子線加速装置は、0.5MeV−5M
eV迄無段階に加速エネルギーを設定でき、カートコンベ
アにより搬送されてくる被処理物に対して上記エネルギ
ーの電子線を照射するようになされているものである。
上記荷姿の培地用合成樹脂製シャーレの滅菌に最適な電
子線の吸収線量としては、150kGyまでが好ましく、150k
Gy以上の吸収線量に設定すると合成樹脂製シャーレに変
形・変色が起こるので好ましくない。尚、合成樹脂製シ
ャーレに付着している雑菌、微生物等が少ない場合に
は、1kGyの吸収線量でも滅菌が可能であるが、実際上は
既述のように実質的吸収線量で10kGy以上にする必要性
がある。尚、この「実質的吸収線量」とは包装容器内に
収容されているシャーレの内で吸収線量が最も少なかっ
たシャーレが受けるべき線量を意味する。The electron beam irradiation in the present invention is performed using an electron beam accelerator. The electron beam accelerator is not particularly limited as long as it emits a high-energy electron beam, and for example, "Dynamitron (mark)" manufactured by Sumitomo Heavy Industries, Ltd. is used. The electron beam accelerator is 0.5 MeV-5M
Acceleration energy can be set steplessly up to eV, and the object to be processed conveyed by the cart conveyor is irradiated with the electron beam of the above energy.
The optimum absorbed dose of the electron beam for the sterilization of the synthetic resin-made petri dish for the medium in the above packaging is preferably up to 150 kGy, and 150 kGy.
It is not preferable to set the absorbed dose to Gy or more because the petri dish made of synthetic resin may be deformed or discolored. Incidentally, if there are few bacteria, microorganisms, etc. attached to the synthetic resin petri dish, it is possible to sterilize even with an absorbed dose of 1 kGy, but in practice, as described above, the actual absorbed dose should be 10 kGy or more. There is a need. The "substantially absorbed dose" means the dose to be received by the petri dish having the smallest absorbed dose among the petri dishes contained in the packaging container.
（実施例） 次に、比較試験例としての実施例により本発明を更に
詳細に説明する。尚、本比較試験例においては、培地と
して血液寒天培地を使用した場合について述べるが、組
織培地、合成培地等についても同様に適用できるもので
あることに留意されたい。(Example) Next, the present invention will be described in more detail with reference to Examples as comparative test examples. In this comparative test example, the case of using a blood agar medium as the medium is described, but it should be noted that the same can be applied to the tissue medium, the synthetic medium and the like.
比較試験例１ 本発明による電子線滅菌シャーレと従来技術によるEO
G滅菌シャーレとを使用して血液寒天培地の保存性への
影響について試験した。Comparative Test Example 1 Electron beam sterilized petri dish according to the present invention and EO according to conventional technology
G sterilized petri dishes were used to test the effect of blood agar on storability.
（１）滅菌方法 電子線滅菌シャーレ ポリスチレン製シャーレをダンボール箱［JIS規格の
丸シャーレ（直径:90mm、高さ:15mm、壁厚:1.0mm）を50
0組収納］に収納し、該ダンボール箱を封緘し、電子線
加速装置（「ダイナミトロン」標章）を用い電子線の加
速エネルギーを5MeV × 16mMに且つカートコンベアの速
度を5m/minに設定してダンボール箱の外側から且つその
上部から１回、次いで該箱の天地を代えて１回電子線を
照射した。電子線の吸収線量は、ダンボール箱内の辺部
のシャーレでは65.8kGyであり、ダンボール箱中心部の
シャーレでは13.9kGyであった。(1) Sterilization method Electron beam sterilized petri dish A polystyrene petri dish is used as a cardboard box [JIS standard round petri dish (diameter: 90 mm, height: 15 mm, wall thickness: 1.0 mm) 50
0 sets], sealed the cardboard box, and set the electron beam acceleration energy to 5 MeV × 16 mM and the cart conveyor speed to 5 m / min using an electron beam accelerator (“Dynamitron” mark). Then, the electron beam was irradiated once from the outside of the cardboard box and from the upper part thereof, and then once again by changing the top and bottom of the box. The absorbed dose of the electron beam was 65.8 kGy in the petri dish in the side of the cardboard box and 13.9 kGy in the petri dish in the center of the cardboard box.
EOG滅菌シャーレ 電子線滅菌用シャーレと同様に、ポリスチレン製シャ
ーレを収容しているダンボール箱をガラス処理室内に載
置し、常法によりEOG20％及び炭酸ガス80％の混合ガス
を0.35kg/cm2の圧力条件下でガス処理室内に送り、温度
35−50℃で4.5−5.0時間処理し、次いでエアレーション
を15−20分間に亘り３回行った。EOG sterilized petri dish As with the electron beam sterilized petri dish, a cardboard box containing a polystyrene petri dish is placed in the glass processing chamber, and 0.35 kg / cm 2 of a mixed gas of 20% EOG and 80% carbon dioxide by a conventional method. Under the pressure condition of
It was treated at 35-50 ° C. for 4.5-5.0 hours, then aerated 3 times for 15-20 minutes.
（２）試験方法 上記の滅菌方法により処理した電子線滅菌シャーレと
EOG滅菌シャーレとを使用して下記に記載の性能試験及
び外観試験を行った。(2) Test method Electron beam sterilized petri dish treated by the above sterilization method
Using the EOG sterilized petri dish, the performance test and appearance test described below were performed.
性能試験 電子線滅菌シャーレ及びEOG滅菌シャーレをダンボー
ル箱からそれぞれ12個取出し、その内で、それぞれ５個
の滅菌シャーレに羊血液寒天培地を、又それぞれ残りの
７個の滅菌シャーレには馬血液寒天培地を分注した。次
いで、それぞれのシャーレに収納された各血液寒天培地
を10℃に保持しながら２ケ月＋３週間保管し、血液寒天
培地の経時的変化を調べた。Performance test Take out 12 electron beam sterilized petri dishes and 12 EOG sterilized petri dishes from the cardboard box, of which 5 sterilized petri dishes each contain sheep blood agar medium, and the remaining 7 sterilized petri dishes each contain horse blood agar. The medium was dispensed. Next, each blood agar medium stored in each petri dish was stored at 10 ° C. for 2 months + 3 weeks, and the change with time of the blood agar medium was examined.
尚、上記それぞれ５個の羊血液寒天培地入りシャーレ
の内４個には、ストレプトコッカスＡ群の菌株を植菌
し、残り１個のシャーレにはストレプトコッカス・ニュ
ーモニアの菌株を植菌した。また、前記それぞれ７個の
馬血液寒天培地入りシャーレの内５個には、上記と同様
にストレプトコッカス属の菌株を植菌し、残り２個のシ
ャーレにヘモフィルス・インフルエンザの菌株を植菌し
た（上記夫々の菌株は、37℃で20−22時間培養したもの
である）。結果は下記の第１表及び第２表に示されてい
る通りであった。Each of the 5 petri dishes containing sheep blood agar medium was inoculated with a strain of Streptococcus group A, and the remaining 1 petri dish was inoculated with a strain of Streptococcus pneumoniae. Further, 5 strains of each of the 7 petri dishes containing horse blood agar were inoculated with a strain of the genus Streptococcus in the same manner as above, and the remaining 2 petri dishes were inoculated with the strain of Haemophilus influenzae (above). Each strain was cultured for 20-22 hours at 37 ° C). The results were as shown in Tables 1 and 2 below.
６−7;ヘモフィルス・インフルエンザ 性能試験結果に関する考察 上記第１表及び第２表に示されている結果から明らか
なように、本発明方法により電子線滅菌されたシャーレ
と従来方法であるEOG滅菌されたシャーレとでは、血液
寒天培地の最も大きな特徴であり且つ重要である「スト
レプトコッカスＡ群のβ溶血性能」及び「ストレプトコ
ッカス・ニューモニアのα溶血性能」の維持に及ぼす影
響において有意差が認められた。 6-7; Consideration on results of Haemophilus influenzae performance test As is clear from the results shown in Tables 1 and 2 above, the petri dish sterilized by the method of the present invention and the EOG sterilization which is the conventional method were sterilized. A significant difference was observed between the petri dish and the petri dish in maintaining the "β-hemolytic performance of Streptococcus group A" and "α-hemolytic performance of Streptococcus pneumoniae", which are the most important and important features of blood agar medium.
即ち、製造直後では両方法により滅菌されたシャーレ
使用血液寒天培地に性能上の差はなかったが、培地収容
シャーレを２ケ月＋３週間保管した後に上記の菌株を植
菌・培養すると、EOG滅菌シャーレ使用血液寒天培地で
は対照培地である製造直後の血液寒天培地とこのストレ
プトコッカスＡ群のβ溶血がＹ溶血化（非溶血）した
り、溶血環が小さくなったり、又集落が小さくなったり
していた。同様に、ストレプトコッカス・ニューモニア
の場合にもα溶血環が小さくなったり、集落が小さくな
ったりしていた。更に、ヘモフィルス・インフルエンザ
の集落も小さくなっていた。これに対して、電子線滅菌
シャーレを用いた場合には、上記の期間に亘り保管した
場合にも、製造直後の血液寒天培地と性能が余り変わら
なかった。従って、本発明による電子線滅菌方法は従来
技術によるEOG滅菌方法よりも有意に優れていることが
判明した。That is, immediately after production, there was no difference in performance between the blood agar medium used in a petri dish sterilized by both methods. The blood agar medium used was a control medium, which was a blood agar medium immediately after production, and β hemolysis of the Streptococcus A group was Y hemolyzed (non-hemolytic), the hemolytic ring was small, and the colony was small. . Similarly, in the case of Streptococcus pneumoniae, the α-hemolytic ring was small and the colony was small. Furthermore, the settlement of Haemophilus influenzae was getting smaller. On the other hand, when the electron beam sterilized petri dish was used, the performance was not so different from that of the blood agar medium immediately after production even when stored for the above period. Therefore, it was found that the electron beam sterilization method according to the present invention is significantly superior to the EOG sterilization method according to the prior art.
外観試験 上記滅菌方法により得た電子線滅菌シャーレ及びEOG
滅菌シャーレをそれぞれ４個用意し、その内、それぞれ
２個のシャーレには羊血液寒天培地を分注して10℃と15
℃に保持し、又残り２個のシャーレには馬血液寒天培地
を分注し10℃と15℃に保持して保管し、これらのシャー
レ内の血液寒天培地の経時的な色調変化を色差計（ミノ
ルタ株式会社製のCM1000）により測定した。この測定
は、各血液寒天入りシャーレを保存温度から室温に戻
し、それを37℃で24時間放置した後に行った。Appearance test Electron beam sterilized petri dish and EOG obtained by the above sterilization method
Prepare four sterile petri dishes each and dispense two sheep petri dishes with sheep blood agar medium at 10 ℃ and 15
℃, the horse blood agar medium was dispensed to the other two petri dishes and kept at 10 ℃ and 15 ℃, and stored. (CM1000 manufactured by Minolta Co., Ltd.). This measurement was performed after each petri dish containing blood agar was returned from the storage temperature to room temperature and left at 37 ° C. for 24 hours.
上記の該色差計は、反射光の分析を行い色調を数値化
するものであり、血液寒天培地の測定に関しては「Ｌ＊
値（明度）及びａ＊値（赤色度）」が重要であり、共に
溶血の度合と黒色化の指標となるものである（溶血或い
は黒色化により上記のＬ＊値及びａ＊値が低下する）。The color difference meter described above is for analyzing reflected light to quantify the color tone. Regarding the measurement of blood agar medium, "L *
“Value (brightness) and a * value (redness)” are important, and both are indicators of the degree of hemolysis and blackening (the above L * and a * values decrease due to hemolysis or blackening). ).
上記色差計により測定した各シャーレ入り血液寒天培
地の経時的な色調変化が横軸に経過月数、縦軸にａ＊値
として、図面第１図−第４図に示されている。The change in color tone with time of each blood agar medium containing petri dishes measured by the color difference meter is shown in FIG. 1 to FIG. 4 as the elapsed months on the horizontal axis and the a * value on the vertical axis.
外観試験結果に関する考察 第１図及び第２図に示されているように電子線滅菌し
たシャーレに収納された羊血液寒天培地は10℃又は15℃
において３ケ月間に亘り保管しても溶血・変色を殆ど生
じないことが判明し、一方EOG滅菌を施したシャーレに
収納された羊血液寒天培地の場合には10℃保管において
約２ケ月後から、又15℃保管においては約１ケ月後から
比較的急速に溶血・変色が生じ、殊に保管温度が高い程
急激に溶血の生じることが判明した。Consideration on appearance test results As shown in Fig. 1 and Fig. 2, sheep blood agar medium stored in a petri dish sterilized by electron beam was 10 ℃ or 15 ℃.
It was found that there was almost no hemolysis or discoloration even after storage for 3 months, while in the case of sheep blood agar medium stored in an EOG sterilized petri dish, it was stored after 10 months storage at about 2 months. Also, it was found that hemolysis and discoloration occurred relatively rapidly after about 1 month when stored at 15 ° C, and that hemolysis occurred rapidly as the storage temperature increased.
馬血液寒天培地に関しては、第３図及び第４図に示さ
れるように、第１図及び第２図に示されている羊血液寒
天培地の場合と比較して溶血・変色は早期に生じるが、
電子線滅菌したシャーレに収容された培地はEOG滅菌し
たシャーレに収容された培地と比較して経時的溶血・変
色の度合が有意に低く、且つ保管温度に殆んど存在しな
いが、EOG滅菌シャーレに収容された培地においては保
管温度が高いと経時的に溶血・変色の度合が著しく高く
なることが判明した。As for horse blood agar medium, as shown in FIGS. 3 and 4, hemolysis and discoloration occur earlier than in the case of sheep blood agar medium shown in FIGS. 1 and 2. ,
The medium contained in an electron beam sterilized petri dish has a significantly lower degree of hemolysis and discoloration over time than the medium contained in an EOG sterilized petri dish, and there is almost no storage temperature. It was found that the medium stored in the medium had a significantly higher degree of hemolysis and discoloration with time when the storage temperature was higher.
尚、図面には示されていないが、Ｌ＊値もａ＊値とほ
ぼ同様に推移した。Although not shown in the drawing, the L * value also changed almost similarly to the a * value.
総合評価 培地用合成樹脂製シャーレの滅菌法を従来のEOG滅菌
から本発明による電子線滅菌に変更すれば、羊血液寒天
培地及び馬血液寒天培地の保管期間を有効に延長するこ
とができる。Comprehensive evaluation If the sterilization method of the synthetic resin petri dish for medium is changed from the conventional EOG sterilization to the electron beam sterilization according to the present invention, the storage period of sheep blood agar medium and horse blood agar medium can be effectively extended.
（発明の効果） 本発明方法によれば下記の通りの効果がもたらされ
る。(Effect of the Invention) According to the method of the present invention, the following effects are brought about.
（１）本発明方法は電子線により合成樹脂製シャーレの
滅菌を行うものであり、この電子線は電圧及び電流の値
を設定することにより加速エネルギーを管理することが
できるために滅菌管理が容易であり、滅菌時間も従来の
Ｙ線滅菌の場合と比較して大幅に短縮し、しかも低コス
トで培地用合成樹脂製シャーレを滅菌することができ
る。(1) The method of the present invention sterilizes a petri dish made of synthetic resin with an electron beam. This electron beam can control acceleration energy by setting voltage and current values, so sterilization control is easy. Therefore, the sterilization time can be significantly shortened as compared with the conventional Y-ray sterilization, and the petri dish made of the synthetic resin for culture medium can be sterilized at low cost.
（２）本発明方法によれば、当該滅菌シャーレを用いて
培地を保管する場合に従来のEOG滅菌したものと比較し
て経時的変化等（色調変化乃至劣化）は著しく少なく、
従って、経時的発育支持能低下を防止乃至抑制すること
ができる。(2) According to the method of the present invention, when the medium is stored using the sterilized petri dish, the change over time (color tone change or deterioration) is significantly less than that of the conventional EOG sterilized,
Therefore, it is possible to prevent or suppress a decrease in growth supporting ability over time.
第１図−第４図は、電子線滅菌又はEOG滅菌された合成
樹脂製シャーレに収容された羊血液寒天培地及び馬血液
寒天培地の経時的な色調変化を示すグラフである。FIGS. 1 to 4 are graphs showing changes in color tone with time of sheep blood agar medium and horse blood agar medium stored in a synthetic resin petri dish sterilized by electron beam or EOG.
───────────────────────────────────────────────────── フロントページの続き (56)参考文献 特開 平１−226528（ＪＰ，Ａ) 特開 昭58−11623（ＪＰ，Ａ) 特開 昭57−142821（ＪＰ，Ａ) 特開 昭49−117181（ＪＰ，Ａ) ─────────────────────────────────────────────────── --- Continuation of the front page (56) References JP-A-1-226528 (JP, A) JP-A-58-11623 (JP, A) JP-A-57-142821 (JP, A) JP-A-49- 117181 (JP, A)
Claims (1)
された培地用合成樹脂製シャーレに、包装容器の外部か
ら電子線を実質的吸収線量で10kGy以上照射することを
特徴とする、培地用合成樹脂製シャーレの滅菌方法。1. A culture medium characterized in that a synthetic resin petri dish for culture medium stacked in a packaging container and accommodated in a predetermined number is irradiated with an electron beam from the outside of the packaging container at a substantial absorbed dose of 10 kGy or more. Method for sterilizing petri dish made of synthetic resin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2208011A JP2553955B2 (en) | 1990-08-08 | 1990-08-08 | Method for sterilizing petri dishes made of synthetic resin for culture medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2208011A JP2553955B2 (en) | 1990-08-08 | 1990-08-08 | Method for sterilizing petri dishes made of synthetic resin for culture medium |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0492671A JPH0492671A (en) | 1992-03-25 |
| JP2553955B2 true JP2553955B2 (en) | 1996-11-13 |
Family
ID=16549195
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2208011A Expired - Lifetime JP2553955B2 (en) | 1990-08-08 | 1990-08-08 | Method for sterilizing petri dishes made of synthetic resin for culture medium |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2553955B2 (en) |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS49117181A (en) * | 1973-03-10 | 1974-11-08 | ||
| JPS57142821A (en) * | 1981-02-28 | 1982-09-03 | Dainippon Printing Co Ltd | Method and device for sterilizing packing material through electron ray |
| JPS6050648B2 (en) * | 1981-06-30 | 1985-11-09 | 大日本印刷株式会社 | Electron beam sterilizer |
| JPH01226528A (en) * | 1988-02-26 | 1989-09-11 | Toppan Printing Co Ltd | Sterilization of packaging material |
-
1990
- 1990-08-08 JP JP2208011A patent/JP2553955B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0492671A (en) | 1992-03-25 |
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