JP2533059B2 - Plasma collection method - Google Patents

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a plasma collection method for collecting plasma components from a blood pool without using a special machine or device.

[0002]

2. Description of the Related Art In recent years, the demand for component transfusion has increased with the advance of medical science. Particularly in Japan, there is a great demand for plasma components, and therefore a large amount of plasma and plasma fractionated preparations are imported, and domestic self-sufficiency is desired. The first method considered for collecting plasma from the blood pool was the so-called centrifugation method in which whole blood was collected from the blood pool, centrifuged to separate it into plasma and blood cell components, and each was collected. In addition, so-called manual plasma component collection methods that return blood cell components to the blood pool to collect only plasma are widely used in other countries, and in addition to the extracorporeal system using an automatic centrifuge that combines a blood pump and a centrifuge. It is also practiced to collect plasma by circulation. The common features of these centrifugation methods are that it cannot be performed without a centrifuge, it is difficult to do it without a power source, and there is a space to place the centrifuge in a narrow space such as in a blood sampling vehicle. It is difficult, the price of the centrifuge is expensive, and white blood cells and platelets are found to be mixed in the collected plasma. Lymphocytes in white blood cells may contain various viruses, and this contamination can lead to serious accidents. Further, in the manual plasma component sampling method, the blood sampling line is once separated from the blood pool, so that it may be mistaken for other blood when returning blood. Moreover, the automatic centrifuge is expensive.

A membrane plasma separator was considered as a method for collecting plasma after the centrifugal separation method. The plasma separated by the membrane plasma separator has a great advantage that white blood cells and platelets are not mixed, and is expected to be put to practical use for plasma collection. Known plasma collection methods using a membrane-type plasma separator include an extracorporeal circulation method using an automatic device including a blood pump, and a drop method represented by the Garland method. However, the method of using an automatic device can not be performed without an automatic device, as in the case of the centrifuge, it is difficult to do it without a power source, and secure a space to put the automatic device in a narrow place. It's tough.

On the other hand, the most representative method of collecting plasma by the drop method is the invention of EPC Publication 014698. This is because the extracorporeal circulation method using a conventional membrane plasma separator requires the operation of a blood pump and various monitors without fail during operation as described above, improving the lack of convenience and improving the blood pump. It is intended to perform extracorporeal circulation without using it and to separate plasma with a device as simple as possible. However, in the device disclosed in EPC Publication 014698,
No attention has been paid to the ease of operation, removal of air in the circuit, closure of the circuit, etc. For example, according to an embodiment, a membrane plasma separator is rinsed with 2000 ml of heparinized saline, a device for injecting a separate anticoagulant is required, and a membrane that has already been rinsed on site is used. The plasma plasma separator is connected to the circuit, and the operation is complicated, and the circuit is not closed. Also, rinsing with a large amount of 2000 ml of heparinized physiological saline isotonic solution for preventing hemolysis, washing eluate, etc. means that a large amount of saline solution is contained in the membrane plasma separator and is collected. The resulting plasma is diluted with this saline, and although the apparent plasma amount obtained is large, only a low protein concentration containing physiological saline can be obtained. Also, with the device of EPC Publication 014698, since blood is collected in a form of passing through a membrane plasma separator,
Blood is subject to the passage resistance of the membrane plasma separator, which takes longer than when blood is directly collected in a blood collection bag, and when blood is returned, the blood cell concentrate passes through the membrane plasma separator again. Therefore, the passing resistance at this time is even greater and it takes time. If the separation ability of the membrane plasma separator is the same, the obtained plasma separation rate is proportional to the blood collection rate, but it is preferable that the blood collection rate be higher in such a device. It is difficult to collect blood through the blood collection method at the blood speed most suitable for the membrane plasma separator to be used, because the blood collection speed becomes low due to the passage resistance and it becomes difficult to obtain a high plasma collection rate. .

Furthermore, as a device for easily collecting plasma,
The invention of EPC Publication 0208061 is known. The device disclosed therein requires a device for measuring the blood sampling rate and a device for controlling the injection of an anticoagulant in conjunction with the device (the former), and a device for measuring the blood sampling rate from a blood pool without using a special device. There are two types: a bag system for directly collecting blood in a blood collection bag containing an anticoagulant, and a membrane plasma collection system (the latter) consisting of a blood transfusion set including a needle, connecting means and a plasma separator at both ends. is there. However, the former requires a special device, EPC release 01146
As in the case of 98, it is necessary to fill the inside of the circuit including the membrane plasma separator with physiological saline or the like in order to remove air in the circuit, rinse the membrane plasma separator, and make the physiological isotonic. Saline is not a preferred method because it lowers the protein concentration of the obtained plasma. The latter requires dilution of the physiological saline solution or the like and connection of a blood collection bag and a blood transfusion set at the site, which prevents the circuit from being closed and may cause contamination from the outside world.

[0006]

SUMMARY OF THE INVENTION The present invention has been made to solve the above-mentioned problems of the prior art. The present invention does not use a special machine or device and continuously injects an anticoagulant. The present invention provides a plasma collection method capable of collecting plasma from a blood pool using only disposable components that keep the system closed as much as possible without performing priming and cleaning of the membrane plasma separator.

[0007]

The gist of the present invention is to collect blood directly from a blood pool (excluding the human body) in a blood collecting bag containing an anticoagulant in advance, and then to partially physiology the blood beforehand. A plasma collection method characterized by introducing into a membrane plasma separator moistened with an isotonic solution, collecting plasma in a blood collection bag, and at the same time returning blood cell concentrated solution from which plasma has been removed to a blood pool. is there. The present invention, in the above plasma collection method, at least a blood collection needle, a blood collection tube connecting the blood collection needle and the blood collection bag, a blood collection bag containing an anticoagulant in advance,
A tube provided with a seal part that connects the blood collection bag and the membrane plasma separator and allows the liquid to flow by breaking it inside, a membrane plasma separation that has been partially moistened with physiological isotonic solution in advance. Device, plasma bag for storing the plasma separated by the membrane plasma separator, tube connecting the plasma separator and the plasma bag, means for removing bubbles in the blood cell concentrated liquid leaving the membrane plasma separator, membrane type A tube connecting the plasma separator and the means for removing air bubbles, a blood return tube for guiding the blood cell concentrated liquid that has exited the means for removing air bubbles to the blood collecting tube for connecting the blood collecting needle and the blood collecting bag, and each of these members is provided. A plasma collection method using a plasma collection unit, characterized in that it forms an integrated closed-system extracorporeal circulation circuit and is sterilized and held in one bag. The present invention is also a plasma collection method using a plasma collection unit in which, in addition to the anticoagulant in the blood collection bag, a sterile gas such as sterile air or sterile nitrogen is sealed. The present invention also provides that the membrane plasma separator has a membrane area of Acm ² of A / 100 (g)
A plasma collection method using a plasma collection unit to which the following physiological isotonic solution is added. The present invention further provides that the physiological isotonic solution is an anticoagulant, and the sum of the amount of this anticoagulant and the amount of the anticoagulant contained in the blood collection bag is the anticoagulant used for normal whole blood sampling. A plasma collection method using a plasma collection unit equal to the amount of coagulant.

As the anticoagulant, a commonly used citric acid-based ACD-A solution or CPD solution is used. The blood collection bag used in the present invention contains an anticoagulant in an amount calculated in advance with respect to a predetermined blood collection amount, and the connecting means (tube) for connecting the blood collection bag and the membrane plasma separator is Before use, it may be closed at a place near the blood collection bag by a seal that allows liquid to flow by breaking. Therefore, the unit used in the present invention does not require a device for continuously injecting the anticoagulant according to blood sampling, and does not require an opening for injecting the anticoagulant.
Further, it is possible to pre-fill a small amount of sterile gas in the blood collection bag, and this gas is not used for sending whole blood collected in the blood collection bag to the membrane plasma separator directly connected to the downstream. Be useful.

The membrane type plasma separator directly connected to the downstream of the blood collecting bag via a tube is not particularly limited in terms of the material and shape of the membrane as long as it can efficiently separate the plasma component and the blood cell component. However, flat membranes or hollow fiber membranes made of cellulose acetate, polyvinyl alcohol, polymethylmethacrylate resin, polyethylene, polypropylene, polysulfone, etc. can be used, but priming and washing operations with physiological solution before use Is not required. However, if the membrane material of the membrane-type plasma separator is hydrophobic, plasma filtration will not occur easily even if blood is introduced if there is no water in the membrane pores, and with a hydrophilic membrane, there will be no water in the pores. Plasma filtration is possible, but hemolysis occurs when blood is introduced. This hemolysis has a membrane area of Acm ² and is A / 1.
It can be prevented by adding a physiological isotonic solution of 00 (g) or less to the membrane pores.

In this way, by pre-wetting the membrane pores that serve as the flow path of the plasma component, both hydrophobic and hydrophilic plasma separation membranes cause problems in performance and hemolysis without washing and priming. Without this, plasma can be smoothly separated, enabling plasma collection to be incorporated into an integrated plasma collection unit. In the plasma collection method according to the present invention, priming need only be performed on the tube used when returning blood to the blood pool, and it is not necessary to wash the membrane plasma separator. Therefore, the collected plasma is hardly diluted with the physiological solution used for priming,
It is possible to obtain high-quality plasma. However,
Pre-wetting the membrane pores of the membrane plasma separator with a physiological isotonic solution is preferable in terms of hemolysis and performance development, but dilutes the obtained plasma component with this physiological isotonic solution. Has drawbacks. Therefore, the physiological isotonic solution added to this membrane hole is used as an anticoagulant, and the amount of the anticoagulant added to the blood collection bag is reduced by the amount added to this membrane hole. Minimization is one feature of the invention. It is preferable that the minimum amount of the liquid added to the membrane pores should be the volume of the membrane pores calculated from theoretical calculation in the case of a hydrophobic membrane, but in a hydrophilic membrane, it is The membrane swells and requires slightly more than a hydrophobic membrane for the same porosity.

When a large amount of the liquid is added to the membrane pores, when the liquid is used as an anticoagulant, the amount of the anticoagulant to be passed to the blood collection bag decreases, and the coagulation system component may be activated during blood collection. However, this is not preferable. In this respect, a plasma separation membrane in which a hydrophobic porous membrane is coated with a water-insoluble hydrophilic polymer is preferable because it hardly swells due to water. In addition, the hydrophobic membrane has a weaker ability to retain water added to the membrane pores than the hydrophilic membrane. From this point as well, a membrane obtained by coating the hydrophobic membrane with a water-insoluble hydrophilic polymer is preferable. In particular, the hydrophobic membrane is preferably a polyolefin such as polyethylene or polypropylene at low cost and various pore diameters can be controlled. The water-insoluble hydrophilic polymer is a copolymer obtained by polymerizing a hydrophobic monomer and a hydrophilic monomer, and the content of the hydrophilic monomer is 40 to
90% by weight is preferable in terms of performance expression. Among them, the plasma separation membrane in which this copolymer is polyethylene vinyl alcohol and the polyolefin is polyethylene is the most preferable because of its high performance. When the physiological isotonic solution is put in advance in the membrane plasma separator used in the present invention, the outlet of the membrane plasma separator is closed by the seal as described above until just before use and does not flow out downstream. You can put it like this.

The blood pool referred to in the present invention means a considerable amount (about 100 ml or more) of blood contained in a container. Specific examples of the container include a blood bag made of a soft resin, a blood tank made of a hard metal (however, having a soft portion for puncturing) such as a non-living body and a human body, a dog,
It also includes animals such as cows, horses and pigs.

In the plasma collection method of the present invention, first, the blood return tube portion connected to the blood pool is primed with a physiological solution such as physiological saline and air is expelled, and then the blood collection needle is inserted into the blood pool (however, (Except) and collect whole blood. At this time, the anticoagulant in the blood collection bag and the blood are mixed, and the blood is anticoagulated. Next, blood is introduced into the membrane plasma separator by breaking the seal provided downstream of the blood collection bag that allows the liquid to flow, and plasma is separated and collected in the plasma bag. Also, the blood cell-rich blood from which plasma has been removed may be collected in a bag and then returned to the blood pool, but the blood cell-rich blood that has left the membrane plasma separator while separating plasma by providing an appropriate head is provided. It is also possible to directly return blood to the blood pool, which is preferable because the time for puncturing the blood pool can be shortened.

When the blood cell-rich blood leaves the membrane plasma separator, it first enters the means for removing air bubbles. This is generally air
What is called a chamber can be used to remove air bubbles during blood return, and the air present in the tube and part of the membrane plasma separator before the means of removing air bubbles from the seal. If the dissolved amount is larger than the amount, it can be used without any problem. The blood cell-rich blood from which bubbles have been removed is sent by a blood return tube into a blood collection tube that connects a blood collection needle and a blood collection bag, and is returned to the blood pool.

The tube for flowing the physiological solution used for priming may be attached to an appropriate portion of this unit. When returning the blood cell-rich blood to be diluted while diluting it with this physiological solution, This tube may be placed just before the means for removing air bubbles, or directly in this means,
Alternatively, it is preferable to connect immediately after this means because it is possible to dilute the hemocyte-rich blood, reduce the resistance at the time of returning blood, and enable smooth plasma collection, and shorten the blood return time, which is preferable. Also, installing the priming tube at the above position
It is easy to expel air by priming only the blood return tube part without priming the membrane type plasma separator,
preferable. In addition, by priming only the blood return tube part, plasma can be collected without wetting the membrane type plasma separator with physiological saline, etc., dilution of the obtained plasma can be prevented, and plasma with high protein concentration can be collected. . A physiological solution such as physiological saline used for priming and blood return may be introduced into this unit by using a bag separate from this unit and puncturing it with a needle. It is also possible to connect to this unit in advance via a seal that allows circulation, and the closing property of the unit is further improved. When collecting plasma from the blood pool by the method of the present invention as described above, first attach the unit to an appropriate position, prime only the tube portion connected to the blood pool with physiological saline, and perform puncture / blood collection. To do. At this time, when collecting blood only by the drop, place the blood collecting bag at an appropriate position below the blood pool and collect by gravity. Further, a so-called suction blood collecting device, which collects blood by decompressing the outside of the blood collecting bag, is often used in blood collecting cars, but it is of course possible to use this. It is also possible to use a tourniquet.

Compared with the conventional method of collecting whole blood in a blood collecting bag through a membrane plasma separator, the method of collecting blood directly from the blood pool of the present invention, which has no passage resistance, provides a high blood collecting speed, The result is a high plasma collection rate. That is, a large amount of plasma can be obtained in a short time. As described above, the present invention can be carried out without using any special machine / device, and a blood collecting device which is often used at the time of collecting blood can also be used. However, all subsequent plasma collection and blood return operations are due to the drop, and can be performed without a power source. However, if it is necessary to suppress the flow velocity, it can be done by pressing the tube with a roller clamp or the like. When it is necessary to accelerate the flow velocity, a blood pump usually used for artificial dialysis or the like can be placed upstream of the membrane plasma separator.

The plasma collection unit for carrying out the present invention is a unit having an extremely closed property that is shielded from the outside air and has only the blood collection needle or only the blood collection needle and the physiological saline introducing needle as the open ends. The needle, the blood collection bag, the membrane plasma separator, the means for removing air bubbles, etc. are integrally connected to each other through a tube as necessary, and similarly, the membrane plasma separator is used. A plasma bag for storing the plasma separated by the container is also connected to this unit.

The unit in which the respective members are integrally connected as described above is sterilized and enclosed in a bag to complete the plasma collection unit of the present invention. In addition, the length of the tube connecting the respective members should be such that a preferable drop can be realized in use and the operation is easy. As a tube for connecting the respective parts, a well-known one made of polyvinyl chloride resin for a blood circuit is used. The tube connecting each part may be flexible, but a hard tube having a length that gives a suitable drop is used, and bending parts etc. are appropriately provided at various places of the tube, and are folded compactly before use, It is also possible to use a device in which each member is arranged at a preferable position during use. The membrane type plasma separator used in the present invention is not particularly limited as described above, but as a particularly preferable one, Japanese Patent Application No. 62-329.
And a small size, high resolution membrane plasma separator as described in 467.

[0019]

Embodiments of the present invention will now be described with reference to the drawings. This device is for collecting plasma components from a blood pool. FIG. 1 shows an embodiment of the present invention which is sterilized and held in a bag (20), and FIG. 2 shows its use state. When in use, the unit is removed from the bag, the head is properly removed and the unit is attached to a suitable support, and then the blood return line is primed. First, remove the cap (16) attached to the saline solution introducing needle (13),
A saline solution introducing needle is inserted into the saline solution bag (21).
Then loosen the roller clamps (18) and (17),
The tube (12), the means (11) for removing air bubbles, the blood return line (14), etc. are filled with physiological saline and air is expelled. Once priming is complete, the roller clamps should be temporarily tightened to prevent saline leakage. Next, by puncturing the blood pool (excluding the human body) with the blood collection needle (1), loosening the roller clamp (19) and destroying it, thereby destroying the seal (3) that allows liquid to flow. Collect an appropriate amount of whole blood. At the time of collecting blood, the blood collecting bag is placed at a position lower than the blood pool and an appropriate head is provided, or blood is collected by a suction blood collecting device or the like. Blood collected through the tube (2) enters the blood collection bag (4). The anticoagulant in the blood collection bag is about 46 ml when CPD is used when 400 ml of whole blood is collected. Normal 400 ml
For whole blood collection, 56 ml of CPD is used, but in this unit, the remaining approximately 10 ml is contained in the membrane plasma separator (7).

When blood collection is completed, a roller clamp (1
Membrane-type plasma separator (7) which passes anticoagulated blood through tube (6) by breaking seal (5) that allows liquid to flow by tightening 9) and then breaking
To be introduced. The plasma separated here is collected in the plasma bag (9) through the tube (8). Further, the blood cell-rich blood from which plasma has been removed enters the means (11) for removing air bubbles through the tube (10), and the blood return tube (14) is loosened by loosening the roller clamp (17).
The blood is collected from the blood collection tube (2) through the blood collection needle and returned to the blood pool. At this time, it is preferable that the roller clamp (18) is loosened a little and physiological saline is dropped to dilute the hemocyte concentrated blood and reduce the resistance passing through the tube or the blood collecting needle, so that blood returning can be smoothly performed.

In the membrane-type plasma separator (7), for example, the surface of the membrane pores of a porous membrane obtained by melting and stretching open pores of high-density polyethylene is coated with polyethylene / vinyl alcohol copolymer to make it hydrophilic. Inner diameter 30 obtained by processing
A membrane containing a hydrophilic composite porous membrane having a membrane area of about 1500 cm ² , which is obtained by bundling 840 hollow fiber membranes having a membrane diameter of 0 μm, a membrane thickness of 40 μm, and an average pore diameter measured by a bubble point method of 0.2 μm. It is preferable to use the one in which 10 ml of CPD solution is placed in the hole portion. In addition, it is possible to enclose sterile air in the blood collection bag, and in this case it is possible to push out the blood remaining in the membrane plasma separator with this air, so collection with physiological saline is omitted. It is convenient because it can be done. From the above, about 5-6 minutes for 400 ml whole blood collection, about 10 minutes for the subsequent plasma separation,
Approximately 15 to 16 minutes with a puncture time of approximately 150 to 200 m
The blood plasma of 1 can be obtained by a very simple operation. Moreover, the protein concentration of the collected plasma is much higher than that of other methods that employ an operation of washing the membrane plasma separator, and the protein recovery rate can be 97% or more. In addition, physiological saline can be dripped into the blood sampling needle punctured in the blood pool (excluding the human body) at any time, and thrombus formation at the needle tip can be prevented. Also, a unit including a plurality of blood collection bags,
Alternatively, if a unit in which an anticoagulant sub-bag is connected to a blood collection bag is produced, it is possible to collect blood plasma multiple times.

[0022]

According to the plasma collection method of the present invention, since no special machine or device is used, the plasma can be collected in the blood collection vehicle or in the field. The unit used in the present invention is a closed unit in which the constituent members are integrated and only the blood sampling needle or only the blood sampling needle and the physiological saline introducing needle can communicate with the outside, and the entire unit is sterilized. Therefore, unlike a conventional device in which each part is connected and assembled at the time of use, hygienic operation with extremely low possibility of contamination of bacteria is possible. In addition, since the operation can be performed with a drop, there is no possibility that air will enter the blood pool (excluding the human body) like a normal drip, and it can be safely performed without a special safety mechanism.
Furthermore, the plasma collection method of the present invention is much easier to prepare for priming and the like than conventional ones, is easy to handle, and obtains high-concentration plasma. Next, the present invention will be specifically described with reference to examples.

[0023]

Example 1 High density polyethylene was used in Japanese Patent Laid-Open No. 61-271.
According to 003, after melting / stretching and opening, the surface of the pores of the porous membrane was coated with polyethylene / vinyl alcohol copolymer and hydrophilized to obtain an inner diameter of 310 μm.
m, membrane thickness 40 μm, and 840 hollow fiber membranes having an average pore diameter of 0.2 μm measured by the bubble point method were bundled to produce a small membrane plasma separator with an effective length of 20 cm and an effective membrane area of about 1600 cm ² . . Membrane type plasma separator is once filled with CPD solution, and then excess CPD solution is removed with pressurized air,
When used, about 10 g of CPD solution remained. Also, 46 ml of CPD solution and 30 ml of air were placed in the blood collection bag, and 16 G of blood collection needle was used. Using these, assemble the plasma collection unit according to Fig. 1 and put it in a sterile bag.
It was sterilized by autoclaving at 20 ° C. for 20 minutes. Prior to use, remove the plasma collection unit from the sterile bag and add 1 saline solution.
1 chamber and 14 blood return lines were filled. Plasma components were collected for adult dog A (Ht = 46, total protein 6.4 g / d
l) was used to puncture the jugular vein, and 400 ml of blood was collected by lowering the blood collection bag below the dog. After that, raise the blood collection bag and the membrane plasma separator up to 80 cm from the puncture site to the lower end of the blood collection bag, and 60 cm from the puncture site to the center of the membrane plasma separator, lower the plasma bag from the membrane plasma separator. It was 55 cm. After that, the seal that allows the liquid to flow through is destroyed, the CPD-added whole blood is introduced into the membrane plasma separator, and the plasma that comes out after filtration is stored in the plasma bag and the plasma is removed. The blood was directly returned to the dog from the puncture site. In this experiment, 180 ml of plasma was collected, the puncture time was 19 minutes, the internal blood collection time was 4 minutes, the plasma separation time was 13 minutes, and the other 2 minutes. The protein concentration of the obtained plasma was 4.95 g / dl. It was As described above, when the plasma collection unit of the present invention is used, it is not necessary to continuously inject the anticoagulant according to the amount of collected blood as in the case of collecting plasma from the blood pool. The labor of washing and priming with water is saved, and since physiological saline is not put into the membrane type plasma separator, physiological saline is not mixed into the collected plasma and the protein concentration is not lowered.

[0024]

Comparative Example 1 Plasma was collected using the plasma collection unit of Example 1. What is different from Example 1 is that 56 ml of a normal amount of CPD solution was put in a blood collection bag and that a membrane-type plasma separator and a return tube were primed before the implementation. One month after carrying out Example 1, the same dog (Ht = 4
7, total protein 6.5 g / dl). As a result, the puncture time was 20 minutes, the internal blood collection time was 4 minutes, and the plasma separation time was 1 minute.
4 minutes, other 2 minutes, the obtained plasma is 205 ml
Then, the total protein became 3.8 g / dl, and the apparent plasma amount increased due to the transfer of the priming solution into the plasma, but the total protein was very low.

[0025]

[Comparative Example 2] The same membrane type plasma separator as in Example 1 was prepared,
The plasma collection unit shown in FIG. 1 was produced. The difference from Example 1 is that 56 ml of CPD solution is put in the blood collection bag,
In addition, the membrane plasma separator was washed with physiological saline prior to the implementation, and the physiological saline discharged to the filtrate side of the membrane plasma separator was discarded. When the membrane plasma separator is washed with physiological saline in this manner, the physiological saline remains inside and outside the hollow fiber membrane.
For this reason, the plasma obtained is diluted as it is, so the filtrate side is opened to the atmosphere (a sterile filter is required for aseptic operation), and a physiological saline disposal line is provided to allow the outside of the membrane to be removed. The physiological saline solution was disposable. Even with this, physiological saline still remains inside the membrane, and there is a possibility of contamination of various bacteria from the physiological saline disposal line. Two months after carrying out Example 1, this comparative example was carried out on the same dog (Ht = 47, total protein 6.5 g / dl). The preparation operation took a lot of time for the above reasons,
Aseptic operation was possible. The puncture time was 20 minutes, the internal blood collection time was 4 minutes, the plasma separation time was 14 minutes, and the other 2 minutes. After puncturing, there was no great difference from Example 1. The obtained plasma is 1
At 92 ml, the protein concentration was 4.50 g / dl, which was considerably lower than that in Example 1.

[0026]

Example 2 The same membrane type plasma separator as in Example 1 was prepared,
The plasma collection unit shown in FIG. 1 was produced. The difference from Example 1 is only the amount of CPD solution in the blood collection bag, which is 40 m.
l. The dog used was a mixed breed dog B (Ht = 41, total protein 6.2 g / dl). In this example, the total CPD amount is 50
Although it is ml, there are no troubles such as the occurrence of blood clots and it is about 21
180 ml of plasma was collected in minutes. The protein concentration of the obtained plasma was 5.00 g / dl.

[0027]

Example 3 The same membrane type plasma separator as in Example 1 was prepared,
The plasma collection unit shown in FIG. 1 was produced. The only difference from Example 1 is that the blood collection needle is 18G. The dog used is a mixed breed dog C. Plasma was collected by the same operation as in Example 1, but physiological saline was added dropwise at a rate of 8 ml / min to the concentrated red blood cells discharged from the membrane plasma separator, unlike in Example 1. In this example, the blood collection needle was thin, and therefore physiological saline was added to the red blood cells to return blood, so that blood plasma could be collected smoothly, and 175 ml of plasma was obtained in about 25 minutes. As a comparison, the dog used in Example 3 one month later was subjected to an experiment without dropping this physiological saline solution. In this experiment, 165 ml of plasma could be collected, but the puncture time was extended to 33 minutes.

[Brief description of drawings]

FIG. 1 is an explanatory view showing an example of a plasma collection unit used in the present invention.

FIG. 2 is an explanatory diagram showing its usage state.

[Explanation of symbols]

 1 Blood collection needle 2 Blood collection tube 3 Seal that allows liquid to flow by breaking 4 Blood collection bag 5 Seal that allows liquid to flow by breaking 6 tube 7 Membrane-type plasma separator 8 tube 9 Plasma bag 10 tube 11 means for removing air bubbles 12 physiological solution introduction tube 13 physiological solution introduction needle 14 blood return tube 15 blood collection needle cap 16 physiological solution introduction needle cap 17 roller clamp 18 roller clamp 19 roller clamp 20 bag 21 Saline bag

Claims (1)

(57) [Claims] 1. A membrane type in which blood is directly collected from a blood pool (excluding the human body) into a blood collection bag containing an anticoagulant in advance, and then the blood is partially preliminarily moistened with a physiological isotonic solution. A method for collecting plasma, which comprises introducing the blood into a plasma separator and collecting the plasma into a plasma bag, and at the same time, returning the blood cell concentrate from which the plasma has been removed to the blood pool.