JP2025517657A - シンシチン-1融合タンパク質及び標的細胞へのカーゴ送達のためのその使用 - Google Patents

シンシチン-1融合タンパク質及び標的細胞へのカーゴ送達のためのその使用 Download PDF

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JP2025517657A
JP2025517657A JP2024566207A JP2024566207A JP2025517657A JP 2025517657 A JP2025517657 A JP 2025517657A JP 2024566207 A JP2024566207 A JP 2024566207A JP 2024566207 A JP2024566207 A JP 2024566207A JP 2025517657 A JP2025517657 A JP 2025517657A
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amino acid
seq
fusion protein
protein
cells
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ミッチョ,アンナリタ
ヴォン・ジョエスト,マチュー
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Assistance Publique Hopitaux de Paris APHP
Fondation Imagine
Universite Paris Cite
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Universite Paris Cite
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JP2024566207A 2022-05-10 2023-05-10 シンシチン-1融合タンパク質及び標的細胞へのカーゴ送達のためのその使用 Pending JP2025517657A (ja)

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Application Number Priority Date Filing Date Title
EP22305688 2022-05-10
EP22305688.8 2022-05-10
PCT/EP2023/062497 WO2023217904A1 (en) 2022-05-10 2023-05-10 Syncitin-1 fusion proteins and uses thereof for cargo delivery into target cells

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JP2025517657A true JP2025517657A (ja) 2025-06-10
JP2025517657A5 JP2025517657A5 (https=) 2026-03-06

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US (1) US20250223326A1 (https=)
EP (1) EP4522630A1 (https=)
JP (1) JP2025517657A (https=)
WO (1) WO2023217904A1 (https=)

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WO2025257403A1 (en) * 2024-06-14 2025-12-18 Dotbio Pte. Ltd. Virus-like particles
CN118557718A (zh) * 2024-08-01 2024-08-30 中国人民解放军军事科学院军事医学研究院 一种以peg10蛋白为载体的纳米颗粒及其制备方法和应用

Family Cites Families (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
US4960716A (en) 1984-05-01 1990-10-02 Ciba Corning Diagnostics Corp. Monoclonal antibodies specific for 330 KD breast tumor antigen and assay using said monoclonal antibodies
US4861719A (en) 1986-04-25 1989-08-29 Fred Hutchinson Cancer Research Center DNA constructs for retrovirus packaging cell lines
US5278056A (en) 1988-02-05 1994-01-11 The Trustees Of Columbia University In The City Of New York Retroviral packaging cell lines and process of using same
EP0368684B2 (en) 1988-11-11 2004-09-29 Medical Research Council Cloning immunoglobulin variable domain sequences.
US5670488A (en) 1992-12-03 1997-09-23 Genzyme Corporation Adenovirus vector for gene therapy
CA2044421C (en) 1990-11-27 2005-06-21 Thomas Hyatt Duffy Squamous cell carcinoma-like immunoreactive antigen from human female urine
AU657102B2 (en) 1991-04-12 1995-03-02 Bio-Rad Laboratories, Inc. CA 195-like immunoreactive antigen from human amniotic fluid
DK1621554T4 (da) 1992-08-21 2012-12-17 Univ Bruxelles Immunoglobuliner blottet for lette kæder
DE69434860T2 (de) 1993-02-22 2007-03-15 The Rockefeller University Herstellung von helfer-freien retroviren mit hohem titer mittels transienter transfektion
FR2712812B1 (fr) 1993-11-23 1996-02-09 Centre Nat Rech Scient Composition pour la production de produits thérapeutiques in vivo.
US6057287A (en) 1994-01-11 2000-05-02 Dyax Corp. Kallikrein-binding "Kunitz domain" proteins and analogues thereof
US6740734B1 (en) 1994-01-14 2004-05-25 Biovitrum Ab Bacterial receptor structures
FR2716459B1 (fr) 1994-02-22 1996-05-10 Univ Paris Curie Système hôte-vecteur utilisable en thérapie génique.
IL116816A (en) 1995-01-20 2003-05-29 Rhone Poulenc Rorer Sa Cell for the production of a defective recombinant adenovirus or an adeno-associated virus and the various uses thereof
EP0739981A1 (en) 1995-04-25 1996-10-30 Vrije Universiteit Brussel Variable fragments of immunoglobulins - use for therapeutic or veterinary purposes
US6013516A (en) 1995-10-06 2000-01-11 The Salk Institute For Biological Studies Vector and method of use for nucleic acid delivery to non-dividing cells
DE19742706B4 (de) 1997-09-26 2013-07-25 Pieris Proteolab Ag Lipocalinmuteine
SE9901379D0 (sv) 1999-04-19 1999-04-19 Pharmacia & Upjohn Ab Receptor structures
US6602977B1 (en) 1999-04-19 2003-08-05 Biovitrum Ab Receptor structures
US20020052040A1 (en) 1999-06-30 2002-05-02 Nicholas Hunt Virus like particles, their preparation and their use preferably in pharmaceutical screening and functional genomics
DE19932688B4 (de) 1999-07-13 2009-10-08 Scil Proteins Gmbh Design von Beta-Faltblatt-Proteinen des gamma-II-kristallins antikörperähnlichen
EP1332209B1 (en) 2000-09-08 2009-11-11 Universität Zürich Collections of repeat proteins comprising repeat modules
US20050053973A1 (en) 2001-04-26 2005-03-10 Avidia Research Institute Novel proteins with targeted binding
US20050089932A1 (en) 2001-04-26 2005-04-28 Avidia Research Institute Novel proteins with targeted binding
US20050048512A1 (en) 2001-04-26 2005-03-03 Avidia Research Institute Combinatorial libraries of monomer domains
US20060223114A1 (en) 2001-04-26 2006-10-05 Avidia Research Institute Protein scaffolds and uses thereof
US20030157561A1 (en) 2001-11-19 2003-08-21 Kolkman Joost A. Combinatorial libraries of monomer domains
US20040175756A1 (en) 2001-04-26 2004-09-09 Avidia Research Institute Methods for using combinatorial libraries of monomer domains
US20030082630A1 (en) 2001-04-26 2003-05-01 Maxygen, Inc. Combinatorial libraries of monomer domains
US20060073141A1 (en) 2001-06-28 2006-04-06 Domantis Limited Compositions and methods for treating inflammatory disorders
DE10324447A1 (de) 2003-05-28 2004-12-30 Scil Proteins Gmbh Generierung künstlicher Bindungsproteine auf der Grundlage von Ubiquitin
US20050164301A1 (en) 2003-10-24 2005-07-28 Avidia Research Institute LDL receptor class A and EGF domain monomers and multimers
DK1773872T3 (en) 2004-05-21 2017-05-08 Uab Res Found VARIABLE Lymphocyte Receptors, Associated Polypeptides and Nucleic Acids, and Uses thereof
US20060008844A1 (en) 2004-06-17 2006-01-12 Avidia Research Institute c-Met kinase binding proteins
US7563443B2 (en) 2004-09-17 2009-07-21 Domantis Limited Monovalent anti-CD40L antibody polypeptides and compositions thereof
US20060234299A1 (en) 2004-11-16 2006-10-19 Avidia Research Institute Protein scaffolds and uses thereof
IL310721B2 (en) 2015-10-23 2025-11-01 Harvard College Nucleobase editors and uses thereof
EP3235828A1 (en) 2016-04-21 2017-10-25 Genethon Stable pseudotyped lentiviral particles and uses thereof
CN110214183A (zh) 2016-08-03 2019-09-06 哈佛大学的校长及成员们 腺苷核碱基编辑器及其用途
EP3284819A1 (en) 2016-08-16 2018-02-21 Max-Delbrück-Centrum für Molekulare Medizin (MDC) Method for the generation of t cell hybridoma for cloning and analysis of receptors using engineered measles virus fusogenic glycoproteins
CA3078096A1 (en) * 2017-10-20 2018-10-19 Genethon Use of syncytin for targeting drug and gene delivery to regenerating muscle tissue
IL273923B2 (en) * 2017-10-20 2024-08-01 Genethon Using syncytin for drug targeting and gene delivery to lung tissue
JP7693552B2 (ja) 2019-02-13 2025-06-17 ビーム セラピューティクス インク. アデノシンデアミナーゼ塩基エディターおよびそれを用いて標的配列中の核酸塩基を改変する方法
US20220184225A1 (en) * 2019-04-23 2022-06-16 Case Western Reserve University Fusogenic particles and related methods for delivering therapeutic agents to cells
AU2020344547A1 (en) 2019-09-09 2022-03-24 Beam Therapeutics Inc. Novel nucleobase editors and methods of using same

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