JP2023548817A - 単一分子検出システムの較正 - Google Patents

単一分子検出システムの較正 Download PDF

Info

Publication number
JP2023548817A
JP2023548817A JP2023525491A JP2023525491A JP2023548817A JP 2023548817 A JP2023548817 A JP 2023548817A JP 2023525491 A JP2023525491 A JP 2023525491A JP 2023525491 A JP2023525491 A JP 2023525491A JP 2023548817 A JP2023548817 A JP 2023548817A
Authority
JP
Japan
Prior art keywords
reference dye
sample
integrated device
amino acids
dye molecule
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
JP2023525491A
Other languages
English (en)
Japanese (ja)
Other versions
JP2023548817A5 (enExample
Inventor
クリスチャン、トーマス
プレストン、カイル
リード、ブライアン
スチューマン、シャノン
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Quantum Si Inc
Original Assignee
Quantum Si Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Quantum Si Inc filed Critical Quantum Si Inc
Publication of JP2023548817A publication Critical patent/JP2023548817A/ja
Publication of JP2023548817A5 publication Critical patent/JP2023548817A5/ja
Withdrawn legal-status Critical Current

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G01N21/6454—Individual samples arranged in a regular 2D-array, e.g. multiwell plates using an integrated detector array
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
    • G01N21/274—Calibration, base line adjustment, drift correction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks

Landscapes

  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Mathematical Physics (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Solid State Image Pick-Up Elements (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
JP2023525491A 2020-10-27 2021-10-26 単一分子検出システムの較正 Withdrawn JP2023548817A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US202063106313P 2020-10-27 2020-10-27
US63/106,313 2020-10-27
US202163152005P 2021-02-22 2021-02-22
US63/152,005 2021-02-22
PCT/US2021/056705 WO2022093859A1 (en) 2020-10-27 2021-10-26 Calibration of single-molecule detection system

Publications (2)

Publication Number Publication Date
JP2023548817A true JP2023548817A (ja) 2023-11-21
JP2023548817A5 JP2023548817A5 (enExample) 2024-11-05

Family

ID=81258250

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2023525491A Withdrawn JP2023548817A (ja) 2020-10-27 2021-10-26 単一分子検出システムの較正

Country Status (6)

Country Link
US (1) US20220128566A1 (enExample)
EP (1) EP4217716A4 (enExample)
JP (1) JP2023548817A (enExample)
AU (1) AU2021372456A1 (enExample)
CA (1) CA3199745A1 (enExample)
WO (1) WO2022093859A1 (enExample)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112020000799A2 (pt) * 2017-07-24 2020-07-14 Quantum-Si Incorporated estruturas fotônicas de rejeição óptica

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050279949A1 (en) * 1999-05-17 2005-12-22 Applera Corporation Temperature control for light-emitting diode stabilization
US20080178653A1 (en) * 2007-01-29 2008-07-31 Applera Corporation System and Method for Interpolative Calibration
US10174363B2 (en) * 2015-05-20 2019-01-08 Quantum-Si Incorporated Methods for nucleic acid sequencing
EP3471402B1 (en) * 2014-08-08 2023-05-31 Quantum-Si Incorporated Integrated device for temporal binning of received photons
CN107407631B (zh) * 2015-02-06 2020-11-03 生命技术公司 用于校准结合染料的系统和方法
WO2016187580A1 (en) * 2015-05-20 2016-11-24 Quantum-Si Incorporated Method of determining the sequence of a nuclear acid using time resolved luminescence
TWI755400B (zh) * 2016-06-01 2022-02-21 美商寬騰矽公司 脈衝命名器及基質命名器、識別核苷酸之方法、校準定序儀器之方法、識別發生核苷酸併入事件之時間的方法、非暫時性電腦可讀儲存媒體、及定序儀器
JP6886854B2 (ja) * 2017-04-13 2021-06-16 シスメックス株式会社 被検物質の情報取得方法
KR20210020864A (ko) * 2018-03-13 2021-02-24 사르말, 인크. 단일 분자 서열분석 방법
WO2020016887A1 (en) * 2018-07-16 2020-01-23 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd Optical devices and methods for measuring efficiency of the fӧrster resonance energy transfer

Also Published As

Publication number Publication date
EP4217716A4 (en) 2024-11-27
AU2021372456A1 (en) 2023-06-08
AU2021372456A9 (en) 2024-06-13
CA3199745A1 (en) 2022-05-05
EP4217716A1 (en) 2023-08-02
WO2022093859A1 (en) 2022-05-05
US20220128566A1 (en) 2022-04-28

Similar Documents

Publication Publication Date Title
US12300711B2 (en) Integrated sensor for lifetime characterization
JP2022539561A (ja) 光学的および電気的な二次経路の除去
CN116670494A (zh) 具有按序耦合电荷存储的集成电路及相关技术
US20220128456A1 (en) Systems and methods for sample process scaling
US20220128469A1 (en) Single molecule detection system using photobleaching information
US12317615B2 (en) Integrated circuit having mirrored pixel configuration
JP2023548817A (ja) 単一分子検出システムの較正
US20250267967A1 (en) Backside illuminated structures with parallel charge transfer
CN116710752A (zh) 利用光漂白信息的单个分子检测系统
US20220344395A1 (en) Photodetector circuit with indirect drain coupling
US20220392932A1 (en) Inter-pixel substrate isolation

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20241025

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20241025

A761 Written withdrawal of application

Free format text: JAPANESE INTERMEDIATE CODE: A761

Effective date: 20250421