JP2023544408A - 操作されたガラクトースオキシダーゼ改変体酵素 - Google Patents

操作されたガラクトースオキシダーゼ改変体酵素 Download PDF

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Publication number
JP2023544408A
JP2023544408A JP2023520447A JP2023520447A JP2023544408A JP 2023544408 A JP2023544408 A JP 2023544408A JP 2023520447 A JP2023520447 A JP 2023520447A JP 2023520447 A JP2023520447 A JP 2023520447A JP 2023544408 A JP2023544408 A JP 2023544408A
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Japan
Prior art keywords
galactose oxidase
sequence
polypeptide
seq
engineered
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JP2023520447A
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Japanese (ja)
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JP2023544408A5 (https=
Inventor
マージー タブガ ボッラ－ガースク，
ジョヴァナ ネイザー，
ナンディタ スブラマニアン，
オスカー アルビゾ，
アンナ フリシュコフスカ，
Original Assignee
コデクシス， インコーポレイテッド
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Publication of JP2023544408A publication Critical patent/JP2023544408A/ja
Publication of JP2023544408A5 publication Critical patent/JP2023544408A5/ja
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/03—Oxidoreductases acting on the CH-OH group of donors (1.1) with a oxygen as acceptor (1.1.3)
    • C12Y101/03009—Galactose oxidase (1.1.3.9)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Ecology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
JP2023520447A 2020-10-06 2021-10-01 操作されたガラクトースオキシダーゼ改変体酵素 Pending JP2023544408A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063087971P 2020-10-06 2020-10-06
US63/087,971 2020-10-06
PCT/US2021/053183 WO2022076263A1 (en) 2020-10-06 2021-10-01 Engineered galactose oxidase variant enzymes

Publications (2)

Publication Number Publication Date
JP2023544408A true JP2023544408A (ja) 2023-10-23
JP2023544408A5 JP2023544408A5 (https=) 2024-10-04

Family

ID=81126807

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2023520447A Pending JP2023544408A (ja) 2020-10-06 2021-10-01 操作されたガラクトースオキシダーゼ改変体酵素

Country Status (8)

Country Link
US (1) US20230374470A1 (https=)
EP (1) EP4225896A4 (https=)
JP (1) JP2023544408A (https=)
CN (1) CN116685678A (https=)
AU (1) AU2021357678A1 (https=)
CA (1) CA3196714A1 (https=)
IL (1) IL301856A (https=)
WO (1) WO2022076263A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116355872B (zh) * 2023-02-23 2023-09-01 中国水产科学研究院黄海水产研究所 半乳糖氧化酶突变体gao-5f/ar、质粒、重组菌及其应用
CN119242604B (zh) * 2024-12-06 2025-04-25 山东理工大学 一种半乳糖氧化酶突变体及其在制备2-氧代-2-呋喃基乙酸中应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020014049A1 (en) * 2018-07-09 2020-01-16 Codexis, Inc. Engineered galactose oxidase variant enzymes

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6090604A (en) * 1999-02-24 2000-07-18 Novo Nordisk Biotech, Inc. Polypeptides having galactose oxidase activity and nucleic acids encoding same
US6498026B2 (en) * 2000-02-25 2002-12-24 Hercules Incorporated Variant galactose oxidase, nucleic acid encoding same, and methods of using same
US7115403B1 (en) * 2000-05-16 2006-10-03 The California Institute Of Technology Directed evolution of galactose oxidase enzymes

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020014049A1 (en) * 2018-07-09 2020-01-16 Codexis, Inc. Engineered galactose oxidase variant enzymes

Also Published As

Publication number Publication date
IL301856A (en) 2023-06-01
EP4225896A4 (en) 2024-11-06
EP4225896A1 (en) 2023-08-16
AU2021357678A1 (en) 2023-04-27
CA3196714A1 (en) 2022-04-14
US20230374470A1 (en) 2023-11-23
CN116685678A (zh) 2023-09-01
WO2022076263A1 (en) 2022-04-14

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