CA3196714A1 - Engineered galactose oxidase variant enzymes - Google Patents

Engineered galactose oxidase variant enzymes

Info

Publication number
CA3196714A1
CA3196714A1 CA3196714A CA3196714A CA3196714A1 CA 3196714 A1 CA3196714 A1 CA 3196714A1 CA 3196714 A CA3196714 A CA 3196714A CA 3196714 A CA3196714 A CA 3196714A CA 3196714 A1 CA3196714 A1 CA 3196714A1
Authority
CA
Canada
Prior art keywords
galactose oxidase
sequence
seq
engineered
polypeptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3196714A
Other languages
English (en)
French (fr)
Inventor
Margie Tabuga Borra-Garske
Jovana Nazor
Nandhitha Subramanian
Oscar Alvizo
Anna FRYSZKOWSKA
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Codexis Inc
Original Assignee
Codexis Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Codexis Inc filed Critical Codexis Inc
Publication of CA3196714A1 publication Critical patent/CA3196714A1/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/03—Oxidoreductases acting on the CH-OH group of donors (1.1) with a oxygen as acceptor (1.1.3)
    • C12Y101/03009—Galactose oxidase (1.1.3.9)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Ecology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
CA3196714A 2020-10-06 2021-10-01 Engineered galactose oxidase variant enzymes Pending CA3196714A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063087971P 2020-10-06 2020-10-06
US63/087,971 2020-10-06
PCT/US2021/053183 WO2022076263A1 (en) 2020-10-06 2021-10-01 Engineered galactose oxidase variant enzymes

Publications (1)

Publication Number Publication Date
CA3196714A1 true CA3196714A1 (en) 2022-04-14

Family

ID=81126807

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3196714A Pending CA3196714A1 (en) 2020-10-06 2021-10-01 Engineered galactose oxidase variant enzymes

Country Status (8)

Country Link
US (1) US20230374470A1 (https=)
EP (1) EP4225896A4 (https=)
JP (1) JP2023544408A (https=)
CN (1) CN116685678A (https=)
AU (1) AU2021357678A1 (https=)
CA (1) CA3196714A1 (https=)
IL (1) IL301856A (https=)
WO (1) WO2022076263A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116355872B (zh) * 2023-02-23 2023-09-01 中国水产科学研究院黄海水产研究所 半乳糖氧化酶突变体gao-5f/ar、质粒、重组菌及其应用
CN119242604B (zh) * 2024-12-06 2025-04-25 山东理工大学 一种半乳糖氧化酶突变体及其在制备2-氧代-2-呋喃基乙酸中应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6090604A (en) * 1999-02-24 2000-07-18 Novo Nordisk Biotech, Inc. Polypeptides having galactose oxidase activity and nucleic acids encoding same
US6498026B2 (en) * 2000-02-25 2002-12-24 Hercules Incorporated Variant galactose oxidase, nucleic acid encoding same, and methods of using same
US7115403B1 (en) * 2000-05-16 2006-10-03 The California Institute Of Technology Directed evolution of galactose oxidase enzymes
SG11202012138XA (en) * 2018-07-09 2021-01-28 Codexis Inc Engineered galactose oxidase variant enzymes

Also Published As

Publication number Publication date
IL301856A (en) 2023-06-01
EP4225896A4 (en) 2024-11-06
JP2023544408A (ja) 2023-10-23
EP4225896A1 (en) 2023-08-16
AU2021357678A1 (en) 2023-04-27
US20230374470A1 (en) 2023-11-23
CN116685678A (zh) 2023-09-01
WO2022076263A1 (en) 2022-04-14

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