JP2023539169A - 二本鎖切断を単離するための方法 - Google Patents

二本鎖切断を単離するための方法 Download PDF

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JP2023539169A
JP2023539169A JP2023512403A JP2023512403A JP2023539169A JP 2023539169 A JP2023539169 A JP 2023539169A JP 2023512403 A JP2023512403 A JP 2023512403A JP 2023512403 A JP2023512403 A JP 2023512403A JP 2023539169 A JP2023539169 A JP 2023539169A
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oligonucleotide
sequence
pair
oligonucleotides
strand
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JPWO2022038291A5 (https=
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サイモン・リード
フェリックス・ダブス
パトリック・ファン・エイク
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ユニバーシティ カレッジ カーディフ コンサルタンツ リミテッド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/301—Endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/307—Single strand endonuclease
    • C—CHEMISTRY; METALLURGY
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/501—Ligase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122—Massive parallel sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
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  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
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  • Microbiology (AREA)
  • Biophysics (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
JP2023512403A 2020-08-21 2021-08-20 二本鎖切断を単離するための方法 Pending JP2023539169A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB2013141.3 2020-08-21
GBGB2013141.3A GB202013141D0 (en) 2020-08-21 2020-08-21 A screening method for the detection of DNA damage
PCT/EP2021/073203 WO2022038291A1 (en) 2020-08-21 2021-08-20 A method for the isolation of double-strand breaks

Publications (3)

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JP2023539169A true JP2023539169A (ja) 2023-09-13
JP2023539169A5 JP2023539169A5 (https=) 2024-08-27
JPWO2022038291A5 JPWO2022038291A5 (https=) 2024-08-27

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US (3) US20240011095A1 (https=)
EP (2) EP4368725B1 (https=)
JP (1) JP2023539169A (https=)
KR (1) KR20230057395A (https=)
CN (1) CN116472356A (https=)
AU (1) AU2021328724A1 (https=)
CA (1) CA3188635A1 (https=)
DK (1) DK4200443T3 (https=)
ES (1) ES2984253T3 (https=)
FI (1) FI4200443T3 (https=)
GB (1) GB202013141D0 (https=)
IL (1) IL300674A (https=)
WO (1) WO2022038291A1 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB202013141D0 (en) 2020-08-21 2020-10-07 Univ College Cardiff Consultants Ltd A screening method for the detection of DNA damage
GB202209189D0 (en) 2022-06-22 2022-08-10 Broken String Biosciences Ltd Methods and compositions for nucleic acid sequencing
EP4569135A1 (en) 2022-08-09 2025-06-18 Institute of Molecular Biology gGmbH (IMB) Method of parallel, rapid and sensitive detection of dna double strand breaks
EP4321630A1 (en) * 2022-08-09 2024-02-14 Institute of Molecular Biology gGmbH (IMB) Method of parallel, rapid and sensitive detection of dna double strand breaks
GB202319988D0 (en) 2023-12-22 2024-02-07 Broken String Biosciences Ltd Methods of detecting dsbs and mutations

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015134552A1 (en) * 2014-03-03 2015-09-11 Swift Biosciences, Inc. Enhanced adaptor ligation
JP2017519508A (ja) * 2014-06-23 2017-07-20 ザ ジェネラル ホスピタル コーポレイション シークエンシングによって評価されるゲノムワイドでバイアスのないＤＳＢの同定（ＧＵＩＤＥ−Ｓｅｑ）
WO2017218979A1 (en) * 2016-06-17 2017-12-21 The Broad Institute, Inc. Unbiased detection of nucleic acid modifications
JP2018530536A (ja) * 2015-09-11 2018-10-18 ザ ジェネラル ホスピタル コーポレイション ヌクレアーゼＤＳＢの完全照合およびシーケンシング（ＦＩＮＤ−ｓｅｑ）
JP2019504618A (ja) * 2015-11-11 2019-02-21 レゾリューション バイオサイエンス， インコーポレイテッド Ｄｎａライブラリーの高効率構築
JP2020505924A (ja) * 2017-01-27 2020-02-27 インテグレイテッド ディエヌエイ テクノロジイズ，インコーポレイテッドＩｎｔｅｇｒａｔｅｄ Ｄｎａ Ｔｅｃｈｎｏｌｏｇｉｅｓ， Ｉｎｃｏｒｐｏｒａｔｅｄ 競合的鎖置換を利用する次世代シーケンシング（ｎｇｓ）ライブラリーの構築

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3722409A1 (en) 2006-03-31 2020-10-14 Illumina, Inc. Systems and devices for sequence by synthesis analysis
CN104195227B (zh) 2008-11-07 2017-04-12 适应生物技术公司 通过序列分析监测状况的方法
WO2011086118A1 (en) 2010-01-14 2011-07-21 Deutsches Krebsforschungszentrum In vivo determination of the lokalization of dna double-stand breaks and application thereof
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
WO2013006745A2 (en) 2011-07-07 2013-01-10 Immune Disease Institute, Inc. High throughput genome-wide translocation sequencing
RU2694686C2 (ru) 2013-03-12 2019-07-16 Е.И.Дюпон Де Немур Энд Компани Способы идентификации вариантных сайтов распознавания для редкощепящих сконструированных средств для индукции двунитевого разрыва, и композиции с ними, и их применения
DK2969479T3 (da) 2013-03-13 2021-08-02 Illumina Inc Væskeanordninger i flere lag og fremgangsmåder til deres fremstilling
WO2016081798A1 (en) 2014-11-20 2016-05-26 Children's Medical Center Corporation Methods relating to the detection of recurrent and non-specific double strand breaks in the genome
EP3234192B1 (en) 2014-12-19 2021-07-14 The Broad Institute, Inc. Unbiased identification of double-strand breaks and genomic rearrangement by genome-wide insert capture sequencing
WO2016138080A1 (en) 2015-02-24 2016-09-01 Trustees Of Boston University Protection of barcodes during dna amplification using molecular hairpins
CA3010199A1 (en) * 2016-02-11 2017-08-17 Htg Molecular Diagnostics, Inc. Method of direct target sequencing using nuclease protection
JP7062681B2 (ja) 2017-03-20 2022-05-06 イルミナ インコーポレイテッド 核酸ライブラリを調製するための方法および組成物
WO2019126774A1 (en) 2017-12-22 2019-06-27 The Broad Institute, Inc. Novel crispr enzymes and systems
WO2019217816A1 (en) * 2018-05-11 2019-11-14 Pioneer Hi-Bred International, Inc. Methods for the identification and characterization of double-strand break sites and compositions and uses thereof
EP3999651B1 (en) 2019-08-26 2026-03-18 Adoc Ssf, Llc Methods of performing guide-seq on primary human t cells
AU2021311713A1 (en) 2020-07-23 2023-03-09 Integrated Dna Technologies, Inc. Methods for nomination of nuclease on-/off-target editing locations, designated "CTL-seq" (CRISPR tag linear-seq)
GB202013141D0 (en) 2020-08-21 2020-10-07 Univ College Cardiff Consultants Ltd A screening method for the detection of DNA damage

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015134552A1 (en) * 2014-03-03 2015-09-11 Swift Biosciences, Inc. Enhanced adaptor ligation
JP2017519508A (ja) * 2014-06-23 2017-07-20 ザ ジェネラル ホスピタル コーポレイション シークエンシングによって評価されるゲノムワイドでバイアスのないＤＳＢの同定（ＧＵＩＤＥ−Ｓｅｑ）
JP2018530536A (ja) * 2015-09-11 2018-10-18 ザ ジェネラル ホスピタル コーポレイション ヌクレアーゼＤＳＢの完全照合およびシーケンシング（ＦＩＮＤ−ｓｅｑ）
JP2019504618A (ja) * 2015-11-11 2019-02-21 レゾリューション バイオサイエンス， インコーポレイテッド Ｄｎａライブラリーの高効率構築
WO2017218979A1 (en) * 2016-06-17 2017-12-21 The Broad Institute, Inc. Unbiased detection of nucleic acid modifications
JP2020505924A (ja) * 2017-01-27 2020-02-27 インテグレイテッド ディエヌエイ テクノロジイズ，インコーポレイテッドＩｎｔｅｇｒａｔｅｄ Ｄｎａ Ｔｅｃｈｎｏｌｏｇｉｅｓ， Ｉｎｃｏｒｐｏｒａｔｅｄ 競合的鎖置換を利用する次世代シーケンシング（ｎｇｓ）ライブラリーの構築

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
MOL. CELL, vol. 78(5), JPN6025040074, 2020, pages 975 - 985, ISSN: 0005697441 *

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EP4368725A2 (en) 2024-05-15
EP4200443B1 (en) 2024-04-10
FI4200443T3 (fi) 2024-07-11
CN116472356A (zh) 2023-07-21
EP4368725A3 (en) 2024-05-29
IL300674A (en) 2023-04-01
AU2021328724A9 (en) 2023-04-27
EP4200443A1 (en) 2023-06-28
US20240309454A1 (en) 2024-09-19
US20240309455A1 (en) 2024-09-19
US20240011095A1 (en) 2024-01-11
ES2984253T3 (es) 2024-10-29
WO2022038291A1 (en) 2022-02-24
CA3188635A1 (en) 2022-02-24
DK4200443T3 (da) 2024-07-22
EP4368725B1 (en) 2026-03-04
AU2021328724A1 (en) 2023-03-30
KR20230057395A (ko) 2023-04-28
US12428683B2 (en) 2025-09-30
US12421554B2 (en) 2025-09-23
GB202013141D0 (en) 2020-10-07

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