JP2023538925A - アデノ随伴ウイルスの収量を増加させるための核酸構築物及びその構築方法 - Google Patents
アデノ随伴ウイルスの収量を増加させるための核酸構築物及びその構築方法 Download PDFInfo
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Abstract
Description
1)AAVエレメントは、Capタンパク質をコードするポリヌクレオチド、Repタンパク質をコードするポリヌクレオチド、及びAAVシスエレメントを含み、例えば、AAVシスエレメントは、好ましくはITR配列である。
2)IEタンパク質をコードするポリヌクレオチドは、Acie0、Acie01又はAcie02のうちの1つ以上から選択される。
3)バキュロウイルスベクター骨格は、pFastBacdual、pFastBac1、pFastBacHTA、pFastBacHTB又はpFastBacHTCのうちの1つから選択される。
4)バキュロウイルス組換え相同領域は、hr1、hr2、hr3、hr4又はhr5のうちの1つ以上から選択される。
1)本発明は、バキュロウイルスベクターに挿入された標的遺伝子の昆虫細胞での発現を明らかに減少させ、昆虫細胞に対する標的遺伝子発現の影響を低減し、産生細胞の増殖指標を改善し、rAAVのパッケージングにより有利である。
2)従来の接着293及び293T細胞の産生、並びに従来のバキュロウイルス産生方法と比較して、この最適化されたベクター構築方法は、単一細胞及び培養物の単位体積あたりのrAAV収量を明らかに増加させ、生産コストを削減し、またスケールアップすることができる。
3)様々な規模、種類のアデノ随伴ウイルス遺伝子治療ベクターの製造など、幅広い応用が可能である。
TTCTCTGTCACAGAATGAAAATTTTTCTGTCATCTCTTCGTTATTAATGTTTGTAATTGACTGAATATCAACGCTTATTTGCAGCCTGAATGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTC
GGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCAGACCAGCCGCGTAACCTGGCAAAATCGGTTACGGTTGAGTAATAAATGGATGCCCTGCGTAAGCGGGTGTGGGCGGACAATAAAGTCTTAAACTGAACAAAATAGATCTAAACTATGACAATAAAGTCTTAAACTAGACAGAATAGTTGTAAACTGAAATCAGTCCAGTTATGCTGTGAAAAAGCATACTGGACTTTTGTTATGGCTAAAGCAAACTCTTCATTTTCTGAAGTGCAAATTGCCCGTCGTATTAAAGAGGGGCGTGGCCAAGGGCATGGTAAAGACTATATTCGCGGCGTTGTGACAATTTACCGAACAACTCCGCGGCCGGGAAGCCGATCTCGGCTTGAACGAATTGTTAGGTGGCGGTACTTGGGTCGATATCAAAGTGCATCACTTCTTCCCGTATGCCCAACTTTGTATAGAGAGCCACTGCGGGATCGTCACCGTAATCTGCTTGCACGTAGATCACATAAGCACCAAGCGCGTTGGCCTCATGCTTGAGGAGATTGATGAGCGCGGTGGCAATGCCCTGCCTCCGGTGCTCGCCGGAGACTGCGAGATCATAGATATAGATCTCACTACGCGGCTGCTCAAACCTGGGCAGAACGTAAGCCGCGAGAGCGCCAACAACCGCTTCTTGGTCGAAGGCAGCAAGCGCGATGAATGTCTTACTACGGAGCAAGTTCCCGAGGTAATCGGAGTCCGGCTGATGTTGGGAGTAGGTGGCTACGTCTCCGAACTCACGACCGAAAAGATCAAGAGCAGCCCGCATGGATTTGACTTGGTCAGGGCCGAGCCTACATGTGCGAATGATGCCCATACTTGAGCCACCTAACTTTGTTTTAGGGCGACTGCCCTGCTGCGTAACATCGTTGCTGCTGCGTAACATCGTTGCTGCTCCATAACATCAAACATCGACCCACGGCGTAACGCGCTTGCTGCTTGGATGCCCGAGGCATAGACTGTACAAAAAAACAGTCATAACAAGCCATGAAAACCGCCACTGCGCCGTTACCACCGCTGCGTTCGGTCAAGGTTCTGGACCAGTTGCGTGAGCGCATACGCTACTTGCATTACAGTTTACGAACCGAACAGGCTTATGTCAACTGGGTTCGTGCCTTCATCCGTTTCCACGGTGTGCGTCACCCGGCAACCTTGGGCAGCAGCGAAGTCGAGGCATTTCTGTCCTGGCTGGCGAACGAGCGCAAGGTTTCGGTCTCCACGCATCGTCAGGCATTGGCGGCCTTGCTGTTCTTCTACGGCAAGGTGCTGTGCACGGATCTGCCCTGGCTTCAGGAGATCGGTAGACCTCGGCCGTCGCGGCGCTTGCCGGTGGTGCTGACCCCGGATGAAGTGGTTCGCATCCTCGGTTTTCTGGAAGGCGAGCATCGTTTGTTCGCCCAGGACTCTAGCTATAGTTCTAGTGGTTGGCCTACGTACCCGTAGTGGCTATGGCAGGGCTTGCCGCCCCGACGTTGGCTGCGAGCCCTGGGCCTTCACCCGAACTTGGGGGTTGGGGTGGGGAAAAGGAAGAAACGCGGGCGTATTGGTCCCAATGGGGTCTCGGTGGGGTATCGACAGAGTGCCAGCCCTGGGACCGAACCCCGCGTTTATGAACAAACGACCCAACACCCGTGCGTTTTATTCTGTCTTTTTATTGCCGTCATAGCGCGGGTTCCTTCCGGTATTGTCTCCTTCCGTGTTTCAGTTAGCCTCCCCCATCTCCCGGTACCGCATGCCTTAGTTGAACTCGAACTTCTTGTACTTGCAGTTGAGCTTTTGCTCGGCGAATGTGATGGCGTCGGAGAGTGGCACCAGTCCCTGCAGGATCAAGGCCAAGAGCTTCAGCAAGTTGTTGTGCAGTGTAGTTGACTCGCGACGGTTGACCTTGCCGATCACGAACATGTGGTGCTTGAATCTGTTGTACTTTTGGA
TGACCTGGCTCACATCAACGTCCTTGATTTCGCCAGAGATCCAGTAGAACTCCTTGTTCTTCTTAGCGATGGTCAGCCTTTCCTCGTTCTTGAAGGACAACACGATGAAGTTGTGACCCTTAACGTTATCGACGTTTTGAGTCAAGTACTGCTCAACGATGTGCATGCTTCCGTCTTCCTTCTTGACCTTCTTGAGGTTCTCGGCGTTGTTGTTAGAAGCGATGTTGTCGTGGTACTTGTAGTTGTTGAAGAGCAGGTTAGCGACGGACGAGTACTTGTATGTCAGAGTGCTCTTCTTGTTCACGATGAGGTTCAGGTTATCAACGACGTACTTGTTGGGAGGGTTGTCCGGGAATTGGACGGACTCCGAGTACTTGAGGATCTGGGAAACGTATGGCGAGACGAAGAAGTTGTTAGAGGCGGTTTCGATCTCGTTTGATTCCTTGCGGCTCAGCATGATGGGCAAAGTGAAGAGGTTCTTGTCCTGGTACATCTCGTACAACTTCGACAAGAGGAATCCACACTTACGCTCGCCGAGTGATTGCAGCAAGGTCACGAAGGTTGTCTGAGCGTAGTACATATCGAGGTTGAAGTAGGAGGTCAGAGCGGCCTTGAATGTGTGGTGGACGTCCACGAAGTGACACTTCTTGCAGTTTTGAGCGGCGGTCTCATCGTTGCAGACGTCCTGTGAGTGTGGGATTTCGATGCCAGTCTCCTTAACCAGGTTGTAGCTGATCATGAAGCGGATCTTATCGAAGGTCACAACGAACACACGGTTGTCAACCATGTAGTAGTTGTTTGTGTACTCGTAGACCACGTTAGAGACGTACTTGGCGAAGATGATTTCGAAAGGCTTCACCTCGGACTTCTTAACGACGAACATGTAGTAACCGGTTTCAGACATGTGATCGGAGAATCTGTTCGAGTTGTAGTCGTTATCGTCGAACCTCATCAGGTAGGGGGCGAAGTCGTTTGTGAAGTAGTGAGTGATCTCCTGGGTGGAAGCCACTGTACAGATGTTAGTGTTGTGGTTGATGGTTTGTTCCAGTGTAGCGCATGACTGGATGGTGCTCTTCTTGTACTTAGGTCTCAACTTGATCTTGTTGAATTGACCCACAACTCCCTGGGAGTTATCCAGGTACTCGTCCAACTTCCTCTTGGTGCCTGTGGCCGACGGCTGGTTGACACCAGCAGAGTGTTCGAAGGACTCGGCGTGGTAAGCTGAGCTAGGAGAGGGTTGTTCGACCACTGGCTGTTCGAGTGACTCGCTGTAGTAGGCAGAGGACACAGCTTCCTCCAGGTTGTCAGTGGTCTTGAGCAGACACTCCACCAAATCGTTGTCAGTGAGCGAGTTAACTGAGGCGAGGAAGTTGCTAGCGGCAGCGGTTTCAGAGTCGGAGATGACAGTATCAGCTCCGTCCGGGGTTGGGTGGTTGTAGTAAGACAAGTAATCGTTAGGTTGCTTGTCGCAGAACTCCGAGTATGAGTTGTCGAAGCTAGCACGAGAGGGAGTGCTGGCAGAGGTGTAGGAAGCGTTGAAGTTGATTTGAGTCATGGTGACCTGGTTGTTCACGATCTTATCGCCACCTGTGTCCACCTGCAGTTGCTGGGCCTCAGCGCAGGCTGAAGTGGCCTCACAGGAGTAGGGGCTGGAAGCACAGTTGGAAGTCATGATGTTTTCTTGGACGTTCAGGACGTGGCTGGATGTACGGATCATGCGCCCGATGGTGGGACGGTATGAATAATCCGGAATATTTATAGGTTTTTTTATTACAAAACTGTTACGAAAACAGTAAAATACTTATTTATTTGCGAGATGGTTATCATTTTAATTATCTCCATGATCGGATCTCCTAGGCTCAAGCAGTGATCAGATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTTGTCTAGTACTTCTCGACGGTACCGCATGCTATGCATCAGCTGCTAGCACCATGGCTCGAGTTACAGATTACGAGTCAGGTATCTGGTGCCAATGGGGCGAGGCTCTGAATACACGCCATTAGTGTCCACAGTAAAGTCCACATTAACAGACTTGTTGTAGTTGGAAGTGTACTGAATTTCGGGATTCCAGCGTTTGCTGTTTTCCTTCTGCAGCTCCCACTCGATCTCCACGCTGACCTGTCCCGTGGAGTACTGTGTGATGAAGGAAGCAAACTTTGCCGCACTGAAGGTGGTCGAAGGATTCGCAGGTACCGGGGTGTTCTTGATGAGAATCTGTGGAGGAGGGTGTTTAAGTCCGAATCCACCCATGAGGGGAGAGGGGTGAAAATGTCCGTCCGTGTGTGGAATCTTTGCCCAGATGGGCCCCTGAAGGTACACATCTCTGTCCTGCCAGACCATGCCTGGAAGAACGCCTTGTGTGTTGACATCTGCGGTAGCTGCTTGTCTGTTGCCTCTCTGGAGGTTGGTAGATACAGAACCATACTGCTCCGTAGCCACGGGATTGGTTGTCCTGATTTCCTCTTCGTCTGTAATCATGACCTTTTCAATGTCCACATTTGTTTTCTCTGAGCCTTGCTTCCCAAAGATGAGAACCCCGCTCTGAGGAAAAAACTTTTCTTCATCGTCCTTGTGGCTTGCCATCGCCGGGCCCGGATTCACCAGAGAGTCTCTGCCATTGAGGTGGTACTTGGTAGCTCCAGTCCACGAGTATTCACTGTTGTTGTTATCCGCAGATGTCTTTGATACTCGCTGCTGGCGGTAACAGGGTCCAGGAAGCCAGTTCCTAGACTGGTCCCGAATGTCACTCGCTCCGGCCTGAGAAAACTGAAGCCTTGACTGCGTGGTGGTTCCACTTGGAGTGTTTGTTCTGCTCAAGTAATACAGGTACTGGTCGATGAGAGGATTCATGAGACGGTCCAGACTCTGGCTGTGAGCGTAGCTGCTGTGGAAAGGAACGTCCTCAAAAGTGTAGCTGAAGGTAAAGTTGTTTCCGGTACGCAGCATCTGAGAAGGAAAGTACTCCAGGCAGTAAAATGAAGAGCGTCCTACTGCCTGACTCCCGTTGTTCAGGGTGAGGTATCCATACTGTGGCACCATGAAGACGTCTGCTGGGAACGGCGGGAGGCATCCTTGATGCGCCGAGCCGAGGACGTACGGGAGCTGGTACTCCGAGTCAGTAAACACCTGAACCGTGCTGGTAAGGTTATTGGCAATCGTCGTCGTACCGTCATTCTGCGTGACCTCTTTGACTTGAATGTTAAAGAGCTTGAAGTTGAGTCTCTTGGGTCGGAATCCCCAGTTGTTGTTGATGAGTCTTTGCCAGTCACGTGGTGAAAAGTGGCAGTGGAATCTGTTGAAGTCAAAATACCCCCAAGGGGTGCTGTAGCCAAAGTAGTGATTGTCGTTCGAGGCTCCTGATTGGCTGGAAATTTGTTTGTAGAGGTGGTTGTTGTAGGTGGGCAGGGCCCAGGTTCGGGTGCTGGTGGTGATGACTCTGTCGCCCATCCATGTGGAATCGCAATGCCAATTTCCCGAGGAATTACCCACTCCGTCGGCGCCCTCGTTATTGTCTGCCATTGGTGCGCCACTGCCTGTAGCCATCGTATTAGTTCCCAGACCAGAGGGGGCTGCTGGTGGCTGTCCGAGAGGCTGGGGGTCAGGTACTGAGTCTGCGTCTCCAGTCTGACCAAAATTCAATCTTTTTCTTGCAGGCTGCTGGCCCGCCTTTCCGGTTCCCGAGGAGGAGTCTGGCTCCACAGGAGAGTGCTCTACCGGCCTCTTTTTTCCCGGAGCCGTCTTAACAGGTTCCTCAACCAGGCCCAGAGGTTCAAGAACCCTCTTTTTCGCCTGGAAGACTGCTCGTCCGAGGTTGCCCCCAAAAGACGTATCTTCTTTAAGGCGCTCCTGGAACT
CCGCGTCGGCGTGGTTGTACTTGAGGTAAGGGTTGTCTCCGCTGTCGAGCTGCCGGTCGTAGGCTTTGTCGTGCTCGAGGGCAGCGGCGTCTGCCTCGTTCACCGGCTCTCCCTTGTCCAGTCCGTTGAAGGGTCCGAGGTACTTGTACCCGGGAAGCACAAGGCCCCTGCTGTCGTCCTTATGCCGCTCTGCGGGCTTTGGTGGTGGTGGGCCGGGTTTGAGCTTCCACCACTGTCTTATTCCTTCAGAGAGAGTGTCCTCGAGCCAATCGGGTAGATAACCGTCGGCAGCCGTGGCGGCTCAAGTCTTCGTCGAGTGATTGTAAATAAAATGTAATTTACAGTATAGTATTTTAATTAATATACAAATGATTTGATAATAATTCTTATTTAACTATAATATATTGTGTTGGGTTGAATTAAAGGTCCGTATCTCCAGACTGCAGTTTAAATTGTGTAATTTATGTAGCTGTAATTTTTACCTTATTAATATTTTTTACGCTTTGCATTCGACGACTGAACTCCCAAATATATGTTTAACTCGTCTTGGTCGTTTGAATTTTTGTTGCTGTGTTTCCTAATATTTTCCATCACCTTAAATATGTTATTGTAATCCTCAATGTTGAACTTGCAATTGGACACGGCATAGTTTTCCATAGTCGTGTAAAACATGGTATTGGCTGCATTGTAATACATCCGACTGAGCGGGTACGGATCTATGTGTTTGAGCAGCCTGTTCAAAAACTCTGCATCGTCGCAAAACGGAATTTGGTCACGATCCCGGTCCGAAGCGCGCGGAATTCAAAGCGCTACGTCAGCGACGTCATCGATTATTGCTCCAATACTAGGGTTTTGTTTTACTATCTGTTCTGCGGTCCACACATCTTCTTCGCTAGTCACGTACGCAAACAACTCGGGCCGCAAAGGTGTTTTAAAACATATCAGATTAGAATCTTTTATAACTTGGCCGCATTGTAAATAGTTGTGCCAACGCGCGGGAAACATTTTACACGTGCTTTCAATCTTATCTCATAAACGCAACTAAATATGATCCTTCAACTCGCTTTACGAGTAGAATTCTACTTGTAACGCACGATTAATTATGACTCATAAGCTGATGTCATGTTTTGCACACGGCTCATAACCGAACTCGCTTTACGAGTAGAATTCTACGCGTAAAACACAATCAAGTATGAGTCATAAGCTGATGTCATGTTTTGCACACGGCTCATAACCGAACTGGCTTTACGAGTAGAATTCTACTTGTAACGCACGATCAGTGGATGATGTCATAAACTTATCTCATGTTTTGCACACGGCTCATAACTTGAGTCATAAGCTGATATCAGAAGATTATTAACGTGTTTAGGTATGACTCATTTGTTTTCAAAACTGAACTCGCTTTACGGATAGAATTCTACTTGTAAAACACAATCAGGGGATGATGTCATTATACAAATGATGTCATTTGTTTTTCAAAACTAAACTCGCTTTACGGGTAGAATTCTACTTGTAAAACACAATCGAGGGATAATGTCATTTGTTATTCTCAAATATGATGTCATTTAAATTTTTATTATGCAAGAATTGTATGTTGCTGTGTCATTTATGAATGTTTTATCGACAACTTGTTTATTTGTTTCATTGACTTGTTGTTGTTCGTGATTATGATTAATTATAAATCTTTTGTTGGGGTCAACATCGATGTATACGCCGCTACAGGGCGCGTACTATGGTTGCTTTGACGTATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCCCCTGCAGGCAGCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCGGGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCCATCACTAGGGGTTCCTACGCGTTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTCAGATCCGCTAGCGCTACCGGTCGCCACCGTCGACGTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATCTAGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTAAAGTTTAAACAGGAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCGGCCTCAGTGAGCGAGCGAGCGCGCAGCTGCCTGCAGGACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCGCGGCCGCTCTAGAGATCATGGAGATAATTAAAATGATAACCATCTCGCAAATAAATAAGTATTTTACTGTTTTCGTAACAGTTTTGTAATAAAAAAACCTATAAATATTCCGGATTATTCATACCGTCCCACCATCGGGCGCGGATCCGCCGCCCTGGCGGGGTTTTACGAGATTGTGATTAAGGTCCCCAGCGACCTTGACGAGCATCTGCCCGGCATTTCTGACAGCTTTGTGAACTGGGTGGCCGAGAAGGAGTGGGAGTTGCCGCCAGATTCTGACTTGGATCTGAATCTGATTGAGCAGGCACCCCTGACCGTGGCCGAGAAGCTGCAGCGCGACTTTCTGACGGAGTGGCGCCGTGTGAGTAAGGCCCCGGAGGCCCTTTTCTTTGTGCAATTTGAGAAGGGAGAGAGCTACTTCCACTTACACGTGCTCGTGGAAACCACCGGGGTGAAATCCTTAGTTTTGGGACGTTTCCTGAGTCAGATTCGCGAAAAACTGATTCAGAGAATTTACCGCGGGATCGAGCCGACTTTGCCAAACTGGTTCGCGGTCACAAAGACCAGAAACGGCGCCGGAGGCGGGAACAAGGTGGTGGACGAGTGCTACATCCCCAATTACTTGCTCCCCAAAACCCAGCCTGAGCTCCAGTGGGCGTGGACTAATTTAGAACAGTATTTAAGCGCCTGTTTGAATCTCACGGAGCGTAAACGGTTGGTGGCGCAGCATCTGACGCACGTGTCGCAGACGCAGGAGCAGAACAAAGAGAATCAGAATCCCAATTCTGACGCGCCGGTGATCAGATCAAAAACTTCAGCCAGGTACATGGAGCTGGTCGGGTGGCTCGTGGACAAGGGGATTACCTCGGAGAAGCAGTGGATACAGGAGGACCAGGCCTCATACATCTCCTTCAATGCGGCCTCCAACTCGCGGTCCCAAATCAAGGCTGCCTTGGACAATGCGGGAAAGATTATGAGCCTGACTAAAACCGCCCCCGACTACC
TGGTGGGCCAGCAGCCCGTGGAGGACATTTCCAGCAATCGGATTTATAAAATTTTGGAACTAAACGGGTACGATCCCCAATATGCGGCTTCCGTCTTTCTGGGATGGGCCACGAAAAAGTTCGGCAAGAGGAACACCATCTGGCTGTTTGGGCCTGCAACTACCGGGAAGACCAACATCGCGGAGGCCATAGCCCACACTGTGCCCTTCTACGGGTGCGTAAACTGGACCAATGAGAACTTTCCCTTCAACGACTGTGTGGACAAGATGGTGATCTGGTGGGAGGAGGGGAAGATGACCGCCAAGGTCGTGGAGTCGGCCAAAGCCATTCTCGGAGGAAGCAAGGTGCGCGTGGACCAGAAATGCAAGTCCTCGGCCCAGATAGACCCGACTCCCGTGATCGTCACCTCCAACACCAACATGTGCGCCGTGATTGACGGGAACTCAACGACCTTCGAACACCAGCAGCCGTTGCAAGACCGGATGTTCAAATTTGAACTCACCCGCCGTCTGGATCATGACTTTGGGAAGGTCACCAAGCAGGAAGTCAAAGACTTTTTCCGGTGGGCAAAGGATCACGTGGTTGAGGTGGAGCATGAATTCTACGTCAAAAAGGGTGGAGCCAAGAAAAGACCCGCCCCCAGTGACGCAGATATAAGTGAGCCCAAACGGGTGCGCGAGTCAGTTGCGCAGCCATCGACGTCAGACGCGGAAGCATCGATCAACTACGCAGACAGGTACCAAAACAAATGTTCTCGTCACGTGGGCATGAATCTGATGCTGTTTCCCTGCAGACAATGCGAGAGAATGAATCAGAATTCAAATATCTGCTTCACTCACGGACAGAAAGACTGTTTAGAGTGCTTTCCCGTGTCAGAATCTCAACCCGTTTCTGTCGTCAAAAAGGCGTATCAGAAACTGTGCTACATTCATCATATCATGGGAAAGGTGCCAGACGCTTGCACTGCCTGCGATCTGGTCAATGTGGATTTGGATGACTGCATCTTTGAACAATAATCTAGAGCCTGCAGTCTCGACAAGCTTGTCGAGAAGTACTAGAGGATCATAATCAGCCATACCACATTTGTAGAGGTTTTACTTGCTTTAAAAAACCTCCCACACCTCCCCCTGAACCTGAAACATAAAATGAATGCAATTGTTGTTGTTAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGGATCTGATCACTGCTTGAGCCTAGGAGATCCGAACCAGATAAGTGAAATCTAGTTCCAAACTATTTTGTCATTTTTAATTTTCGTATTAGCTTACGACGCTACACCCAGTTCCCATCTATTTTGTCACTCTTCCCTAAATAATCCTTAAAAACTCCATTTCCACCCCTCCCAGTTCCCAACTATTTTGTCCGCCCACAGCGGGGCATTTTTCTTCCTGTTATGTTTTTAATCAAACATCCTGCCAACTCCATGTGACAAACCGTCATCTTCGGCTACTTT
DNA複製開始及び組換えAAVビリオンのパッケージングに必要な唯一の既知のシスエレメントであると考えられる。
ATGATAAGAACCAGCAGTCACGTGCTGAACGTCCAGGAAAATATAATGACGTCAAACTGTGCGTCATCGCCATATTCGTGCGAGGCAACGTCCGCTTGCGCAGAAGCTCAGCAGGTAATGATCGATAACTTTGTTTTCTTTCACATGTACAACGCCGACATACAAATTGACGCAAAGCTGCAATGCGGCGTGCGCTCGGCCGCGTTTGCAATGATCGACGATAAACATTTGGAAATGTACAAGCATAGAATAGAGAATAAATTTTTTTATTACTATGATCAATGTGCCGACATTGCCAAACCCGACCGTCTGCCCGATGACGACGGCGCGTGCTGTCACCATTTTATTTTTGATGCCCAACGTATTATTCAATGTATTAAAGAGATTGAAAGCGCGTACGGCGTGCGTGATCGCGGCAATGTAATAGTGTTTTATCCGTACTTGAAACAGTTGCGAGACGCGTTGAAGCTAATTAAAAACTCTTTTGCGTGTTGTTTTAAAATTATAAATTCTATGCAAATGTACGTGAACGAGTTAATATCAAATTGCCTGTTGTTTATTGAAAAGCTGGAAACTATTAATAAAACTGTTAAAGTTATGAATTTGTTTGTAGACAATTTGGTTTTGTACGAATGCAATGTTTGTAAAGAAATATCTACGGATGAAAGATTTTTAAAGCCAAAAGAATGTTGCGAATACGCTATATGCAACGCGTGCTGCGTTAACATGTGGAAGACGGCCACCACGCACGCAAAATGTCCAGCGTGCAGGACATCGTATAAATAA
MIRTSSHVLNVQENIMTSNCASSPYSCEATSACAEAQQVMIDNFVFFHMYNADIQIDAKLQCGVRSAAFAMIDDKHLEMYKHRIENKFFYYYDQCADIAKPDRLPDDDGACCHHFIFDAQRIIQCIKEIESAYGVRDRGNVIVFYPYLKQLRDALKLIKNSFACCFKIINSMQMYVNELISNCLLFIEKLETINKTVKVMNLFVDNLVLYECNVCKEISTDERFLKPKECCEYAICNACCVNMWKTATTHAKCPACRTSYK
ATGATCCGTACATCCAGCCACGTCCTGAACGTCCAAGAAAACATCATGACTTCCAACTGTGCTTCCAGCCCCTACTCCTGTGAGGCCACTTCAGCCTGCGCTGAGGCCCAGCAACTGCAGGTGGACACAGGTGGCGATAAGATCGTGAACAACCAGGTCACCATGACTCAAATCAACTTCAACGCTTCCTACACCTCTGCCAGCACTCCCTCTCGTGCTAGCTTCGACAACTCATACTCGGAGTTCTGCGACAAGCAACCTAACGATTACTTGTCTTACTACAACCACCCAACCCCGGACGGAGCTGATACTGTCATCTCCGACTCTGAAACCGCTGCCGCTAGCAACTTCCTCGCCTCAGTTAACTCGCTCACTGACAACGATTTGGTGGAGTGTCTGCTCAAGACCACTGACAACCTGGAGGAAGCTGTGTCCTCTGCCTACTACAGCGAGTCACTCGAACAGCCAGTGGTCGAACAACCCTCTCCTAGCTCAGCTTACCACGCCGAGTCCTTCGAACACTCTGCTGGTGTCAACCAGCCGTCGGCCACAGGCACCAAGAGGAAGTTGGACGAGTACCTGGATAACTCCCAGGGAGTTGTGGGTCAATTCAACAAGATCAAGTTGAGACCTAAGTACAAGAAGAGCACCATCCAGTCATGCGCTACACTGGAACAAACCATCAACCACAACACTAACATCTGTACAGTGGCTTCCACCCAGGAGATCACTCACTACTTCACAAACGACTTCGCCCCCTACCTGATGAGGTTCGACGATAACGACTACAACTCGAACAGATTCTCCGATCACATGTCTGAAACCGGTTACTACATGTTCGTCGTTAAGAAGTCCGAGGTGAAGCCTTTCGAAATCATCTTCGCCAAGTACGTCTCTAACGTGGTCTACGAGTACACAAACAACTACTACATGGTTGACAACCGTGTGTTCGTTGTGACCTTCGATAAGATCCGCTTCATGATCAGCTACAACCTGGTTAAGGAGACTGGCATCGAAATCCCACACTCACAGGACGTCTGCAACGATGAGACCGCCGCTCAAAACTGCAAGAAGTGTCACTTCGTGGACGTCCACCACACATTCAAGGCCGCTCTGACCTCCTACTTCAACCTCGATATGTACTACGCTCAGACAACCTTCGTGACCTTGCTGCAATCACTCGGCGAGCGTAAGTGTGGATTCCTCTTGTCGAAGTTGTACGAGATGTACCAGGACAAGAACCTCTTCACTTTGCCCATCATGCTGAGCCGCAAGGAATCAAACGAGATCGAAACCGCCTCTAACAACTTCTTCGTCTCGCCATACGTTTCCCAGATCCTCAAGTACTCGGAGTCCGTCCAATTCCCGGACAACCCTCCCAACAAGTACGTCGTTGATAACCTGAACCTCATCGTGAACAAGAAGAGCACTCTGACATACAAGTACTCGTCCGTCGCTAACCTGCTCTTCAACAACTACAAGTACCACGACAACATCGCTTCTAACAACAACGCCGAGAACCTCAAGAAGGTCAAGAAGGAAGACGGAAGCATGCACATCGTTGAGCAGTACTTGACTCAAAACGTCGATAACGTTAAGGGTCACAACTTCATCGTGTTGTCCTTCAAGAACGAGGAAAGGCTGACCATCGCTAAGAAGAACAAGGAGTTCTACTGGATCTCTGGCGAAATCAAGGACGTTGATGTGAGCCAGGTCATCCAAAAGTACAACAGATTCAAGCACCACATGTTCGTGATCGGCAAGGTCAACCGTCGCGAGTCAACTACACTGCACAACAACTTGCTGAAGCTCTTGGCCTTGATCCTGCAGGGACTGGTGCCACTCTCCGACGCCATCACATTCGCCGAGCAAAAGCTCAACTGCAAGTACAAGAAGTTCGAGTTCAACTAA
MIRTSSHVLNVQENIMTSNCASSPYSCEATSACAEAQQLQVDTGGDKIVNNQVTMTQINFNASYTSASTPSRASFDNSYSEFCDKQPNDYLSYYNHPTPDGADTVISDSETAAASNFLASVNSLTDNDLVECLLKTTDNLEEAVSSAYYSESLEQPVVEQPSPSSAYHAESFEHSAGVNQPSATGTKRKLDEYLDNSQGVVGQFNKIKLRPKYKKSTIQSCATLEQTINHNTNICTVASTQEITHYFTNDFAPYLMRFDDNDYNSNRFSDHMSETGYYMFVVKKSEVKPFEIIFAKYVSNVVYEYTNNYYMVDNRVFVVTFDKIRFMISYNLVKETGIEIPHSQDVCNDETAAQNCKKCHFVDVHHTFKAALTSYFNLDMYYAQTTFVTLLQSLGERKCG
FLLSKLYEMYQDKNLFTLPIMLSRKESNEIETASNNFFVSPYVSQILKYSESVQFPDNPPNKYVVDNLNLIVNKKSTLTYKYSSVANLLFNNYKYHDNIASNNNAENLKKVKKEDGSMHIVEQYLTQNVDNVKGHNFIVLSFKNEERLTIAKKNKEFYWISGEIKDVDVSQVIQKYNRFKHHMFVIGKVNRRESTTLHNNLLKLLALILQGLVPLSDAITFAEQKLNCKYKKFEFN
ATGAGTCGCCAAATCAACGCCGCCACTCCCAGCAGCAGCCGCCGCCACAGGCTGTCTCTCAGCCGTCGCCGCATCAACTTTACAACATCTCCCGAAGCCCAGCCGTCTTCAAGCAGTCGCAGCCAGCCGTCTTCAAGCAGTCGCAGCCATCGCCGTCAGGAGCGGCGTCAGGAGCAGCGTGTCAGCGAAGAAAACGTGCAGATTATCGGGAACGTCAACGAGCCGTTGACGCGCACCTACCATCGTCAGGGTGTCACGTATTACGTGCACGGTCAGGTTAACATTAGCAATGACGATCCGCTATTAAGTCAAGAGGATGACGTCATACTAATTAATAGTGAAAATGTGGATCGTGAACGGTTTCCCGACATCACTGCCCAGCAGTACCAGGATAACATTGCGTCGGAGACAGCTGCGCAGAGGGCTCTGCAACGAGGTTTAGATCTTGAGGCTCAGCTGATGAATGAGATTGCCCCAAGGTCTCCCACTTATAGTCCATCTTATTCGCCGAATTACGTAATACCACAGTCGCCAGATTTGTTTGCCTCGCCGCAGTCTCCGCAGCCGCAGCAGCAGCAGCAGCAGCAATCAGAACCCGAAGAAGAAGTAGAGGTTTCGTGTAATATTTGTTTTACTACTTTTAAAGACACTAAAAACGTAAATTCCTCGTTTGTGACTTCGATTCATTGTAACCATGCTGTGTGTTTCAAGTGTTATGTCAAGATAATTATGGACAATTCTGTGTACAAATGTTTTTGCAGCGCTACTTCATCAGATTGTCGCGTGTACAATAAGCACGGGTATGTAGAATTTATGCCCATTAACGTCACTCGTAACCAGGATTCCATCAAACAGCATTGGCGCGAGCTTTTAGAAAATAACACGGTCAACAATCACACCACGGACTTGAACTATGTGGAGCAATTGCAAAAAGAACTGTCCGAGCTGCGAGCCAAGACCAGCCAAGTTGAACATAAAATGACCATGTTAAACAGCGACTACATTATGCTTAAACACAAGCATGCTGTCGCCGAATTAGATTTACAAAAGGCAAACTATGACTTGCAAGAATCTACCAAGAAATCAGAAGAGTTGCAATCGACTGTGAATAATCTGCAAGAACAATTGCGTAAGCAGGTGGCCGAGTCTCAAGCCAAATTTTCAGAGTTTGAGCGCAGTAACTCTGATTTAGTTTCTAAGTTACAAACTGTTATGTCTAGACGTTAA
MSRQINAATPSSSRRHRLSLSRRRINFTTSPEAQPSSSSRSQPSSSSRSHRRQERRQEQRVSEENVQIIGNVNEPLTRTYHRQGVTYYVHGQVNISNDDPLLSQEDDVILINSENVDRERFPDITAQQYQDNIASETAAQRALQRGLDLEAQLMNEIAPRSPTYSPSYSPNYVIPQSPDLFASPQSPQPQQQQQQQSEPEEEVEVSCNICFTTFKDTKNVNSSFVTSIHCNHAVCFKCYVKIIMDNSVYKCFCSATSSDCRVYNKHGYVEFMPINVTRNQDSIKQHWRELLENNTVNNHTTDLNYVEQLQKELSELRAKTSQVEHKMTMLNSDYIMLKHKHAVAELDLQKANYDLQESTKKSEELQSTVNNLQEQLRKQVAESQAKFSEFERSNSDLVSKLQTVMSRR
ATCGATGTTGACCCCAACAAAAGATTTATAATTAATCATAATCACGAACAACAACAAGTCAATGAAACAAATAAACAAGTTGTCGATAAAACATTCATAAATGACACAGCAACATACAATTCTTGCATAATAAAAATTTAAATGACATCATATTTGAGAATAACAAATGACATTATCCCTCGATTGTGTTTTACAAGTAGAATTCTACCCGTAAAGCGAGTTTAGTTTTGAAAAACAAATGACATCATTTGTATAATGACATCATCCCCTGATTGTGTTTTACAAGTAGAATTCTATCCGTAAAGCGAGTTCAGTTTTGA
AAACAAATGAGTCATACCTAAACACGTTAATAATCTTCTGATATCAGCTTATGACTCAAGTTATGAGCCGTGTGCAAAACATGAGATAAGTTTATGACATCATCCACTGATCGTGCGTTACAAGTAGAATTCTACTCGTAAAGCCAGTTCGGTTATGAGCCGTGTGCAAAACATGACATCAGCTTATGACTCATACTTGATTGTGTTTTACGCGTAGAATTCTACTCGTAAAGCGAGTTCGGTTATGAGCCGTGTGCAAAACATGACATCAGCTTATGAGTCATAATTAATCGTGCGTTACAAGTAGAATTCTACTCGTAAAGCGAGTTGAAGGATCATATTTAGTTGCGTTTATGAGATAAGATTGAAAGCACGTGTAAAATGTTTCCCGCGCGTTGGCACAACTATTTACAATGCGGCCAAGTTATAAAAGATTCTAATCTGATATGTTTTAAAACACCTTTGCGGCCCGAGTTGTTTGCGTACGTGACTAGCGAAGAAGATGTGTGGACCGCAGAACAGATAGTAAAACAAAACCCTAGTATTGGAGCAATAATCGAT
TGAGCAAAACACAACCGGCAAATTCTCGGCGGCCGTTTGGGAATGCGGAATAATTGCCATATGTAAATGATGTCATCGGTTCTAACTCGCTTTACGAGTAGAATTCTACGTGTAAAACATAATCAAGAGATGATGTCATTTGTTTTTCAAAACTGAACTCAAGAAATGATGTCATTTGTTTTTCAAAACTGAACTGGCTTTACGAGTAGAATTCTACTTGTAAAACACAATCGAGAGATGATGTCATATTTTGCACACGGCTCTAATTAAACTCGCTTTACGAGTAAAATTCTACTTGTAACGCATGATCAAGGGATGATGTCATTGGATGAGTCATTTGTTTTTCAAAACTAAACTCGCTTTACGAGTAGAATTCTACTTGTAAAACACAATCAAGGGATGATGTCATTATACAAATGATGTCATTTGTTTTTCAAAACTAAACTCGCTTTACGGGTAGAATTCTACTTGTAAAACAGCAACTCGAGGGATGATGTCATCCTTTACTCGATGATTATAAACGTGTTTATGTATGACTCATTTGTTTTTCAAAACTAAACTCGCTTTACGAGTAGAATTCTACTTGTAACGCACGATCAAGGGATGATGTCATTTATTTGTGCAAAGCTCGATGTCATCTTTTGCACACGATTATAAACACAATCCAAATAATGACTCATTTGTTTTCAAAACTGAACTCGCTTTACGAGTAGAATTCTACTTGTAAAACACAATCAAGGGATGATGTCATTTTCAAAATGATGTCATTTGTTTTTCAAAACTAAACTCGCTTTACGAGTAGAATTCTACTTGTAAAACACAATCAAGGGATGATGTCATTTTAAAAATGATCATTTGTTTTTCAAAACTAAACTCGCTTTACGAGTAGAATTCTACGTGTAAAACACAATCAAGGGATGATGTCATTTACTAAATAAAATAATTATTTAAATAAAACTGTTTTTTATTGTCAAATACACATTGATTCAC
ACGCGTAGAATTCTACTTGTAAAGCAAGTTAAAATAAGCCGTGTGCAAAAATGACATCAGACAAATGACATCATCTACCTATCATGATCATGTTAATAATCATGTTTTAAAATGACATCAGCTTATGACTAATAATTGATCGTGCGTTACAAGTAGAATTCTACTCGTAAAGCGAGTTTAGTTTTGAAAACAAATGAGTCATCATTAAACATGTTAATAATCGTGTATAAAGGATGACATCATCCACTAATCGTGCGTTACAAGTAGAATTCTACTCGTAAAGCGAGTTCGGTTTTGAAAAACAAATGACATCATTTCTTGATTGTGTTTTACACGTAGAATTCTACTCGTAAAGTATGTTCAGTTTAAAAAACAAATGACATCATTTTACAGATGACATCATTTCTTGATTATGTTTTACAAGTAGAATTCTACTCGTAAAGCGAGTTTAGTTTTAAAAAACAAATGACATCATCTCTTGATTATGTTTTACAAGTAGAATTCTACTCGTAAAGCGAGTTTAGTTTTGAAAAACAAATGACATCATCTCTTGATTATGTTTTACAAGTAGAATTCTACTCGTAAAGCGAGTTTAGTTTTGAAAAACAAATGACATCATCCCTTGATCATGCGTTACAAGTAGAATTCTACTCGTAAAGCGAGTTGAATTTTGATTACAATATT
ATGCATATAATTGTGTACAAAATATGACTCATTAATCGATCGTGCGTTACAAGTAGAATTCTACTGGTAAAGCAAGTTCGGTTGTGAGCCGTGTGCAAAACATGACATCATAACTAATCATGTTTATAATCATGTGCAAAATATGACATCATCCGACGATTGTGTTTTACAAGTAGAATTCTACTCGTAAAGCGAGTTTAAAAATTTTGTGACGTCAATGAAACAACGTGTAATATTTTTTACAATATTTAAGTGAAACATTATGACTTCCAATAATTTTGTGGATGTGGATACGTTTGCAAGACAATTGATTACAGATAAATGTAGTGCTCTAATCGAAAGATGCGGATCTGTTGCCGGCAAACATTTTAGAGATTAGTAGAGAAAGGCCAGAGACAAGTATTTTGAGGTGCCAACTCAAAAAAACTATGAATACATTAAAAAATTATTTTTACGAACAAAATATATGGACGATTCGATAGATTATAAAGATTTTAACAGACGCATCCTATTGATAGTTTTTAAATTCGCTTTAAACAAGAGCACCAACTACTTTCCATCGTACTAAAGAGATCATCGAGGTGGCCATTAAACGTTTAAACAAAATTAACCCCGATTTAAAGAGTTCTCCGCGCAATGCTTCAGCATTACAAATGAATGTTTGGAAAATCTAGA
AACTGGCTTTACGAGTAGAATTCTACTTGTAAAACACAATCAAGAAATGATGTCATTTTTGTACGTGATTATAAACATGTTTAAACATGGTACATTGAACTTAATTTTTGCAAGTTGATAAACTAGATTAATGTATGACTCATTTGTTTGTGCAAGTTGATAAACGTGATTAATATATGACTCATATGTTTGTGCAAAAATGGTGTCATCGTACAAACTCGCTTTACGAGTAGAATTCTA
CTTGTAAAACACAATCGAGGGATGATGTCATTTGTAGAATGATGTCATTTGTTTTTTCAAAACCGAACTCGCTTTACGAGTAGAATTCTACTTGTAAAACACAATCGAGGGATGATGTCATTTGTAGAATGATGTCATCGTACAAACTCGCTTTACGAGTAGAATTCTAGTAAAACAC
TTGAAAATTTATTGCCTAATATTATTTTTGTCAGTTCGTTGTCATTTATTAATTTGGATGATGTCCATTTGTTTTTAAAATTGAACTGGCTTTACGAGTAGAATTCTACGCGTAAAACACAATCAAGTATGAGTCATAAGCTGATGTCATGTTTTGCACACGGCTCATAACCGAACTGGCTTTACGAGTAGAATTCTACTTGTAACGCACGATCGAGTGGATGATGGTCATTTGTTTTTCAAATCGAGATGATGTCATGTTTTGCACACGGGCTCATAAACTGCTTTACGAGTAGAATTCTACGTGTAACGCACGATCGATTGATGAGTCATTTGTTTTGCAATATGATATCATACAATATGACTCATTTGTTTTTCAAAACCGAACTTGATTTACGGGTAGAATTCTACTCGTAAAGCACAATCAAAAAGATGATGTCATTTGTTTTTCAAAACTGAACTCTCGGCTTTACGAGTAGAATTCTACGTGTAAAACACAATCAAGAAATGATGTCATTTGTTATAAAAATAAAAGCTGATGTCATGTTTTGCACATGGCTCATAACTAAACTCGCTTTACGGGTAGAATTCTACGCGTAAAACATGATTGATAATTAAATAATTCATTTGCAAAGCTATACGTTAAATCAAACGGACGTTATGGAATTGTATAATATTAAATATGCAATTGATCCAACAAATAAAATTATAATAGAGCAAGTCGAC
ュロウイルスベクターである。いくつかの実施形態では、組換えバキュロウイルスベクターは、組換えバキュロウイルスシャトルベクターである。
ITR Forward primer 5’-GGAACCCCTAGTGATGGAGTT-3’(配列番号2)
ITR Reverse primer 5’-CGGCCTCAGTGAGCGA-3’(配列番号3)
レメントを含む核酸構築物、IEタンパク質をコードするポリヌクレオチドを含む核酸構築物、バキュロウイルス組換え相同領域をコードするポリヌクレオチドを含む核酸構築物を指す。AAVエレメントを含む核酸構築物はまた、Capタンパク質をコードするポリヌクレオチドを含む核酸構築物、Repタンパク質をコードするポリヌクレオチドを含む核酸構築物、AAVシスエレメントをコードするポリヌクレオチドを含む核酸構築物から選択することができる。組換えバキュロウイルスは、上記の核酸構築物をバキュロウイルス系によって共同構築することによって得ることができる。
選択される。
1)AAVエレメントは、Capタンパク質をコードするポリヌクレオチド、Repタンパク質をコードするポリヌクレオチド、及びAAVシスエレメントを含み、AAVシスエレメントは、好ましくはITR配列である。
2)IEタンパク質は、Acie0、Acie01又はAcie02遺伝子のうちの1つ以上によってコードされる。
3)バキュロウイルスベクター骨格は、pFastBacdual、pFastBac1、pFastBacHTA、pFastBacHTB又はpFastBacHTCのうちの1つから選択される。
4)バキュロウイルス組換え相同領域は、hr1、hr2、hr3、hr4又はhr5のうちの1つ以上から選択される。
5)バキュロウイルス組換え相同領域をコードするポリヌクレオチドをバキュロウイルスベクター骨格に組み込む。
関連遺伝子の決定:Capタンパク質及びRepタンパク質の遺伝子はいずれもAAV2ゲノム(GenBank:AF043303.1)に由来し、リボソームリーキースキャニングメカニズムに基づいて、文献(非特許文献2)を参照して塩基の点突然変異を行った。Acie01(GenBank:NC_001623.1)、バキュロウイルス組換え相同領域hr1(GenBank:M14313.1)。
号9のヌクレオチド配列)をそれぞれ得た。
ACGGCTGCCGACGGTTATCTACCCGATTGGCTCGAGGACACTCTCTCTGAAGGAATAAGACAGTGGTGGAAGCTCAAACCCGGCCCACCACCACCAAAGCCCGCAGAGCGGCATAAGGACGACAGCAGGGGCCTTGTGCTTCCCGGGTACAAGTACCTCGGACCCTTCAACGGACTGGACAAGGGAGAGCCGGTGAACGAGGCAGACGCCGCTGCCCTCGAGCACGACAAAGCCTACGACCGGCAGCTCGACAGCGGAGACAACCCTTACCTCAAGTACAACCACGCCGACGCGGAGTTCCAGGAGCGCCTTAAAGAAGATACGTCTTTTGGGGGCAACCTCGGACGAGCAGTCTTCCAGGCGAAAAAGAGGGTTCTTGAACCTCTGGGCCTGGTTGAGGAACCTGTTAAGACGGCTCCGGGAAAAAAGAGGCCGGTAGAGCACTCTCCTGTGGAGCCAGACTCCTCCTCGGGAACCGGAAAGGCGGGCCAGCAGCCTGCAAGAAAAAGATTGAATTTTGGTCAGACTGGAGACGCAGACTCAGTACCTGACCCCCAGCCTCTCGGACAGCCACCAGCAGCCCCCTCTGGTCTGGGAACTAATACGATGGCTACAGGCAGTGGCGCACCAATGGCAGACAATAACGAGGGCGCCGACGGAGTGGGTAATTCCTCGGGAAATTGGCATTGCGATTCCACATGGATGGGCGACAGAGTCATCACCACCAGCACCCGAACCTGGGCCCTGCCCACCTACAACAACCACCTCTACAAACAAATTTCCAGCCAATCAGGAGCCTCGAACGACAATCACTACTTTGGCTACAGCACCCCTTGGGGGTATTTTGACTTCAACAGATTCCACTGCCACTTTTCACCACGTGACTGGCAAAGACTCATCAACAACAACTGGGGATTCCGACCCAAGAGACTCAACTTCAAGCTCTTTAACATTCAAGTCAAAGAGGTCACGCAGAATGACGGTACGACGACGATTGCCAATAACCTTACCAGCACGGTTCAGGTGTTTACTGACTCGGAGTACCAGCTCCCGTACGTCCTCGGCTCGGCGCATCAAGGATGCCTCCCGCCGTTCCCAGCAGACGTCTTCATGGTGCCACAGTATGGATACCTCACCCTGAACAACGGGAGTCAGGCAGTAGGACGCTCTTCATTTTACTGCCTGGAGTACTTTCCTTCTCAGATGCTGCGTACCGGAAACAACTTTACCTTCAGCTACACTTTTGAGGACGTTCCTTTCCACAGCAGCTACGCTCACAGCCAGAGTCTGGACCGTCTCATGAATCCTCTCATCGACCAGTACCTGTATTACTTGAGCAGAACAAACACTCCAAGTGGAACCACCACGCAGTCAAGGCTTCAGTTTTCTCAGGCCGGAGCGAGTGACATTCGGGACCAGTCTAGGAACTGGCTTCCTGGACCCTGTTACCGCCAGCAGCGAGTATCAAAGACATCTGCGGATAACAACAACAGTGAATACTCGTGGACTGGAGCTACCAAGTACCACCTCAATGGCAGAGACTCTCTGGTGAATCCGGGCCCGGCGATGGCAAGCCACAAGGACGATGAAGAAAAGTTTTTTCCTCAGAGCGGGGTTCTCATCTTTGGGAAGCAAGGCTCAGAGAAAACAAATGTGGACATTGAAAAGGTCATGATTACAGACGAAGAGGAAATCAGGACAACCAATCCCGTGGCTACGGAGCAGTATGGTTCTGTATCTACCAACCTCCAGAGAGGCAACAGACAAGCAGCTACCGCAGATGTCAACACACAAGGCGTTCTTCCAGGCATGGTCTGGCAGGACAGAGATGTGTACCTTCAGGGGCCCATCTGGGCAAAGATTCCACACACGGACGGACATTTTCACCCCTCTCCCCTCATGGGTGGATTCGGACTTAAACACCCTCCTCCACAGATTCTCATCAAGAACACCCCGGTACCTGCGAATCCTTCGACCACCTTCAGTGCGGCAAAGTTTGCTTCCTTCATCACACAGTACTCCACGGGACAGGTCAGCGTGGAGATCGAGTGGGAGCTGCAGAAGGAAAACAGCAAACGCTGGAATCCCGAAATTCAGTACACTTCCAACTACAACAAGTCTGTTAATGTGGACTTTACTGTGGACACTAATGGCGTGTATTCAGAGCCTCGCCCCATTGGCACCAGATACCTGACTCGTAATCTGTAA
CTGGCGGGGTTTTACGAGATTGTGATTAAGGTCCCCAGCGACCTTGACGAGCATCTGCCCGGCATTTCTGACAGCTTTGTGAACTGGGTGGCCGAGAAGGAGTGGGAGTTGCCGCCAGATTCTGACTTGGATCTGAATCTGATTGAGCAGGCACCCCTGACCGTGGCCGAGAAGCTGCAGCGCGACTTTCTGACGGAGTGGCGCCGTGTGAGTAAGGCCCCGGAGGCCCTTTTCTTTGTGCAATTTGAGAAGGGAGAGAGCTACTTCCACTTACACGTGCTCGTGGAAACCACCGGGGTGAAATCCTTAGTTTTGGGACGTTTCCTGAGTCAGATTCGCGAAAAACTGATTCAGAGAATTTACCGCGGGATCGAGCCGACTTTGCCAAACTGGTTCGCGGTCACAAAGACCAGAAACGGCGCCGGAGGCGGGAACAAGGTGGTGGACGAGTGCTACATCCCCAATTACTTGCTCCCCAAAACCCAGCCTGAGCTCCAGTGGGCGTGGACTAATTTAGAACAGTATTTAAGCGCCTGTTTGAATCTCACGGAGCGTAAACGGTTGGTGGCGCAGCATCTGACGCACGTGTCGCAGACGCAGGAGCAGAACAAAGAGAATCAGAATCCCAATTCTGACGCGCCGGTGATCAGATCAAAAACTTCAGCCAGGTACATGGAGCTGGTCGGGTGGCTCGTGGACAAGGGGATTACCTCGGAGAAGCAGTGGATACAGGAGGACCAGGCCTCATACATCTCCTTCAATGCGGCCTCCAACTCGCGGTCCCAAATCAAGGCTGCCTTGGACAATGCGGGAAAGATTATGAGCCTGACTAAAACCGCCCCCGACTACCTGGTGGGCCAGCAGCCCGTGGAGGACATTTCCAGCAATCGGATTTATAAAATTTTGGAACTAAACGGGTACGATCCCCAATATGCGGCTTCCGTCTTTCTGGGATGGGCCACGAAAAAGTTCGGCAAGAGGAACACCATCTGGCTGTTTGGGCCTGCAACTACCGGGAAGACCAACATCGCGGAGGCCATAGCCCACACTGTGCCCTTCTACGGGTGCGTAAACTGGACCAATGAGAACTTTCCCTTCAACGACTGTGTGGACAAGATGGTGATCTGGTGGGAGGAGGGGAAGATGACCGCCAAGGTCGTGGAGTCGGCCAAAGCCATTCTCGGAGGAAGCAAGGTGCGC
GTGGACCAGAAATGCAAGTCCTCGGCCCAGATAGACCCGACTCCCGTGATCGTCACCTCCAACACCAACATGTGCGCCGTGATTGACGGGAACTCAACGACCTTCGAACACCAGCAGCCGTTGCAAGACCGGATGTTCAAATTTGAACTCACCCGCCGTCTGGATCATGACTTTGGGAAGGTCACCAAGCAGGAAGTCAAAGACTTTTTCCGGTGGGCAAAGGATCACGTGGTTGAGGTGGAGCATGAATTCTACGTCAAAAAGGGTGGAGCCAAGAAAAGACCCGCCCCCAGTGACGCAGATATAAGTGAGCCCAAACGGGTGCGCGAGTCAGTTGCGCAGCCATCGACGTCAGACGCGGAAGCATCGATCAACTACGCAGACAGGTACCAAAACAAATGTTCTCGTCACGTGGGCATGAATCTGATGCTGTTTCCCTGCAGACAATGCGAGAGAATGAATCAGAATTCAAATATCTGCTTCACTCACGGACAGAAAGACTGTTTAGAGTGCTTTCCCGTGTCAGAATCTCAACCCGTTTCTGTCGTCAAAAAGGCGTATCAGAAACTGTGCTACATTCATCATATCATGGGAAAGGTGCCAGACGCTTGCACTGCCTGCGATCTGGTCAATGTGGATTTGGATGACTGCATCTTTGAACAATAA
ATCGATGTTGACCCCAACAAAAGATTTATAATTAATCATAATCACGAACAACAACAAGTCAATGAAACAAATAAACAAGTTGTCGATAAAACATTCATAAATGACACAGCAACATACAATTCTTGCATAATAAAAATTTAAATGACATCATATTTGAGAATAACAAATGACATTATCCCTCGATTGTGTTTTACAAGTAGAATTCTACCCGTAAAGCGAGTTTAGTTTTGAAAAACAAATGACATCATTTGTATAATGACATCATCCCCTGATTGTGTTTTACAAGTAGAATTCTATCCGTAAAGCGAGTTCAGTTTTGAAAACAAATGAGTCATACCTAAACACGTTAATAATCTTCTGATATCAGCTTATGACTCAAGTTATGAGCCGTGTGCAAAACATGAGATAAGTTTATGACATCATCCACTGATCGTGCGTTACAAGTAGAATTCTACTCGTAAAGCCAGTTCGGTTATGAGCCGTGTGCAAAACATGACATCAGCTTATGACTCATACTTGATTGTGTTTTACGCGTAGAATTCTACTCGTAAAGCGAGTTCGGTTATGAGCCGTGTGCAAAACATGACATCAGCTTATGAGTCATAATTAATCGTGCGTTACAAGTAGAATTCTACTCGTAAAGCGAGTTGAAGGATCATATTTAGTTGCGTTTATGAGATAAGATTGAAAGCACGTGTAAAATGTTTCCCGCGCGTTGGCACAACTATTTACAATGCGGCCAAGTTATAAAAGATTCTAATCTGATATGTTTTAAAACACCTTTGCGGCCCGAGTTGTTTGCGTACGTGACTAGCGAAGAAGATGTGTGGACCGCAGAACAGATAGTAAAACAAAACCCTAGTATTGGAGCAATAATCGATGACGTCGCTGACGTAGCGCTTTGAATTCCGCGCGCTTCGGACCGGGATCGTGACCAAATTCCGTTTTGCGACGATGCAGAGTTTTTGAACAGGCTGCTCAAACACATAGATCCGTACCCGCTCAGTCGGATGTATTACAATGCAGCCAATACCATGTTTTACACGACTATGGAAAACTATGCCGTGTCCAATTGCAAGTTCAACATTGAGGATTACAATAACATATTTAAGGTGATGGAAAATATTAGGAAACACAGCAACAAAAATTCAAACGACCAAGACGAGTTAAACATATATTTGGGAGTTCAGTCGTCGAATGCAAAGCGTAAAAAATATTAATAAGGTAAAAATTACAGCTACATAAATTACACAATTTAAACTGCAGTCTGGAGATACGGACCTTTAATTCAACCCAACACAATATATTATAGTTAAATAAGAATTATTATCAAATCATTTGTATATTAATTAAAATACTATACTGTAAATTACATTTTATTTACAATC
AACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGGATCTGATCACTGCTTGAGCCTAGGAGATCCGATCATGGAGATAATTAAAATGATAACCATCTCGCAAATAAATAAGTATTTTACTGTTTTCGTAACAGTTTTGTAATAAAAAAACCTATAAATATTCCGGATTATTCATACCGTCCCACCATCGGGCGCATGATCCGTACATCCAGCCACGTCCTGAACGTCCAAGAAAACATCATGACTTCCAACTGTGCTTCCAGCCCCTACTCCTGTGAGGCCACTTCAGCCTGCGCTGAGGCCCAGCAACTGCAGGTGGACACAGGTGGCGATAAGATCGTGAACAACCAGGTCACCATGACTCAAATCAACTTCAACGCTTCCTACACCTCTGCCAGCACTCCCTCTCGTGCTAGCTTCGACAACTCATACTCGGAGTTCTGCGACAAGCAACCTAACGATTACTTGTCTTACTACAACCACCCAACCCCGGACGGAGCTGATACTGTCATCTCCGACTCTGAAACCGCTGCCGCTAGCAACTTCCTCGCCTCAGTTAACTCGCTCACTGACAACGATTTGGTGGAGTGTCTGCTCAAGACCACTGACAACCTGGAGGAAGCTGTGTCCTCTGCCTACTACAGCGAGTCACTCGAACAGCCAGTGGTCGAACAACCCTCTCCTAGCTCAGCTTACCACGCCGAGTCCTTCGAACACTCTGCTGGTGTCAACCAGCCGTCGGCCACAGGCACCAAGAGGAAGTTGGACGAGTACCTGGATAACTCCCAGGGAGTTGTGGGTCAATTCAACAAGATCAAGTTGAGACCTAAGTACAAGAAGAGCACCATCCAGTCATGCGCTACACTGGAACAAACCATCAACCACAACACTAACATCTGTACAGTGGCTTCCACCCAGGAGATCACTCACTACTTCACAAACGACTTCGCCCCCTACCTGATGAGGTTCGACGATAACGACTACAACTCGAACAGATTCTCCGATCACATGTCTGAAACCGGTTACTACATGTTCGTCGTTAAGAAGTCCGAGGTGAAGCCTTTCGAAATCATCTTCGCCAAGTACGTCTCTAACGTGGTCTACGAGTACACAAACAACTACTACATGGTTGACAACCGTGTGTTCGTTGTGACCTTCGATAAGATCCGCTTCATGATCAGCTACAACCTGGTTAAGGAGACTGGCATCGAAATCCCACACTCACAGGACGTCTGCAACGATGAGACCGCCGCTCAAAACTGCAAGAAGTGTCACTTCGTGGACGTCCACCACACATTCAAGGCCGCTCTGACCTCCTACTTCAACCTCGATATGTACTACGCTCAGACAACCTTCGTGACCTTGCTGCAATCACTCGGCGAGCGTAAGTGTGGATTCCTCTTGTCGAAGTTGTACGAGATGTA
CCAGGACAAGAACCTCTTCACTTTGCCCATCATGCTGAGCCGCAAGGAATCAAACGAGATCGAAACCGCCTCTAACAACTTCTTCGTCTCGCCATACGTTTCCCAGATCCTCAAGTACTCGGAGTCCGTCCAATTCCCGGACAACCCTCCCAACAAGTACGTCGTTGATAACCTGAACCTCATCGTGAACAAGAAGAGCACTCTGACATACAAGTACTCGTCCGTCGCTAACCTGCTCTTCAACAACTACAAGTACCACGACAACATCGCTTCTAACAACAACGCCGAGAACCTCAAGAAGGTCAAGAAGGAAGACGGAAGCATGCACATCGTTGAGCAGTACTTGACTCAAAACGTCGATAACGTTAAGGGTCACAACTTCATCGTGTTGTCCTTCAAGAACGAGGAAAGGCTGACCATCGCTAAGAAGAACAAGGAGTTCTACTGGATCTCTGGCGAAATCAAGGACGTTGATGTGAGCCAGGTCATCCAAAAGTACAACAGATTCAAGCACCACATGTTCGTGATCGGCAAGGTCAACCGTCGCGAGTCAACTACACTGCACAACAACTTGCTGAAGCTCTTGGCCTTGATCCTGCAGGGACTGGTGCCACTCTCCGACGCCATCACATTCGCCGAGCAAAAGCTCAACTGCAAGTACAAGAAGTTCGAGTTCAACTAA
TTCTCTGTCACAGAATGAAAATTTTTCTGTCATCTCTTCGTTATTAATGTTTGTAATTGACTGAATATCAACGCTTATTTGCAGCCTGAATGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCAGACCAGCCGCGTAACCTGGCAAAATCGGTTACGGTTGAGTAATAAATGGATGCCCTGCGTAAGCGGGTGTGGGCGGACAATAAAGTCTTAAACTGAACAAAATAGATCTAAACTATGACAATAAAGTCTTAAACTAGACAGAATAGTTGTAAACTGAAATCAGTCCAGTTATGCTGTGAAAAAGCATACTGGACTTTTGTTATGGCTAAAGCAAACTCTTCATTTTCTGAAGTGCAAATTGCCCGTCGTATTAAAGAGGGGCGTGGCCAAGGGCATGGTAAAGACTATATTCGCGGCGTTGTGACAATTTACCGAACAACTCCGCGGCCGGGAAGCCGATCTCGGCTTGAACGAATTGTTAGGTGGCGGTACTTGGGTCGATATCAAAGTGCATCACTTCTTCCCGTATGCCCAACTTTGTATAGAGAGCCACTGCGGGATCGTCACCGTAATCTGCTTGCACGTAGATCACATAAGCACCAAGCGCGTTGGCCTCATGCTTGAGGAGATTGATGAGCGCGGTGGCAATGCCCTGCCTCCGGTGCTCGCCGGAGACTGCGAGATCATAGATATAGAT
CTCACTACGCGGCTGCTCAAACCTGGGCAGAACGTAAGCCGCGAGAGCGCCAACAACCGCTTCTTGGTCGAAGGCAGCAAGCGCGATGAATGTCTTACTACGGAGCAAGTTCCCGAGGTAATCGGAGTCCGGCTGATGTTGGGAGTAGGTGGCTACGTCTCCGAACTCACGACCGAAAAGATCAAGAGCAGCCCGCATGGATTTGACTTGGTCAGGGCCGAGCCTACATGTGCGAATGATGCCCATACTTGAGCCACCTAACTTTGTTTTAGGGCGACTGCCCTGCTGCGTAACATCGTTGCTGCTGCGTAACATCGTTGCTGCTCCATAACATCAAACATCGACCCACGGCGTAACGCGCTTGCTGCTTGGATGCCCGAGGCATAGACTGTACAAAAAAACAGTCATAACAAGCCATGAAAACCGCCACTGCGCCGTTACCACCGCTGCGTTCGGTCAAGGTTCTGGACCAGTTGCGTGAGCGCATACGCTACTTGCATTACAGTTTACGAACCGAACAGGCTTATGTCAACTGGGTTCGTGCCTTCATCCGTTTCCACGGTGTGCGTCACCCGGCAACCTTGGGCAGCAGCGAAGTCGAGGCATTTCTGTCCTGGCTGGCGAACGAGCGCAAGGTTTCGGTCTCCACGCATCGTCAGGCATTGGCGGCCTTGCTGTTCTTCTACGGCAAGGTGCTGTGCACGGATCTGCCCTGGCTTCAGGAGATCGGTAGACCTCGGCCGTCGCGGCGCTTGCCGGTGGTGCTGACCCCGGATGAAGTGGTTCGCATCCTCGGTTTTCTGGAAGGCGAGCATCGTTTGTTCGCCCAGGACTCTAGCTATAGTTCTAGTGGTTGGCCTACGTACCCGTAGTGGCTATGGCAGGGCTTGCCGCCCCGACGTTGGCTGCGAGCCCTGGGCCTTCACCCGAACTTGGGGGTTGGGGTGGGGAAAAGGAAGAAACGCGGGCGTATTGGTCCCAATGGGGTCTCGGTGGGGTATCGACAGAGTGCCAGCCCTGGGACCGAACCCCGCGTTTATGAACAAACGACCCAACACCCGTGCGTTTTATTCTGTCTTTTTATTGCCGTCATAGCGCGGGTTCCTTCCGGTATTGTCTCCTTCCGTGTTTCAGTTAGCCTCCCCCATCTCCCGGTACCGCATGCTTACAGATTACGAGTCAGGTATCTGGTGCCAATGGGGCGAGGCTCTGAATACACGCCATTAGTGTCCACAGTAAAGTCCACATTAACAGACTTGTTGTAGTTGGAAGTGTACTGAATTTCGGGATTCCAGCGTTTGCTGTTTTCCTTCTGCAGCTCCCACTCGATCTCCACGCTGACCTGTCCCGTGGAGTACTGTGTGATGAAGGAAGCAAACTTTGCCGCACTGAAGGTGGTCGAAGGATTCGCAGGTACCGGGGTGTTCTTGATGAGAATCTGTGGAGGAGGGTGTTTAAGTCCGAATCCACCCATGAGGGGAGAGGGGTGAAAATGTCCGTCCGTGTGTGGAATCTTTGCCCAGATGGGCCCCTGAAGGTACACATCTCTGTCCTGCCAGACCATGCCTGGAAGAACGCCTTGTGTGTTGACATCTGCGGTAGCTGCTTGTCTGTTGCCTCTCTGGAGGTTGGTAGATACAGAACCATACTGCTCCGTAGCCACGGGATTGGTTGTCCTGATTTCCTCTTCGTCTGTAATCATGACCTTTTCAATGTCCACATTTGTTTTCTCTGAGCCTTGCTTCCCAAAGATGAGAACCCCGCTCTGAGGAAAAAACTTTTCTTCATCGTCCTTGTGGCTTGCCATCGCCGGGCCCGGATTCACCAGAGAGTCTCTGCCATTGAGGTGGTACTTGGTAGCTCCAGTCCACGAGTATTCACTGTTGTTGTTATCCGCAGATGTCTTTGATACTCGCTGCTGGCGGTAACAGGGTCCAGGAAGCCAGTTCCTAGACTGGTCCCGAATGTCACTCGCTCCGGCCTGAGAAAACTGAAGCCTTGACTGCGTGGTGGTTCCACTTGGAGTGTTTGTTCTGCTCAAGTAATACAGGTACTGGTCGATGAGAGGATTCATGAGACGGTCCAGACTCTGGCTGTGAGCGTAGCTGCTGTGGAAAGGAACGTCCTCAAAAGTGTAGCTGAAGGTAAAGTTGTTTCCGGTACGCAGCATCTGAGAAGGAAAGTACTCCAGGCAGTAAAATGAAGAGCGTCCTACTGCCTGACTCCCGTTGTTCAGGGTGAGGTATCCATACTGTGGCACCATGAAGACGTCTGCTGGGAACGGCGGGAGGCATCCTTGATGCGCCGAGCCGAGGACGTACGGGAGCTGGTACTCCGAGTCAGTAAACACCTGAACCGTGCTGGTAAGGTTATTGGCAATCGTCGTCGTACCGTCATTCTGCGTGACCTCTTTGACTTGAATGTTAAAGAGCTTGAAGTTGAGTCTCTTGGGTCGGAATCCCCAGTTGTTGTTGATGAGTCTTTGCCAGTCACGTGGTGAAAAGTGGCAGTGGAATCTGTTGAAGTCAAAATACCCCCAAGGGGTGCTGTAGCCAAAGTAGTGATTGTCGTTCGAGGCTCCTGATTGGCTGGAAATTTGTTTGTAGAGGTGGTTGTTGTAGGTGGGCAGGGCCCAGGTTCGGGTGCTGGTGGTGATGACTCTGTCGCCCATCCATGTGGAATCGCAATGCCAATTTCCCGAGGAATTACCCACTCCGTCGGCGCCCTCGTTATTGTCTGCCATTGGTGCGCCACTGCCTGTAGCCATCGTATTAGTTCCCAGACCAGAGGGGGCTGCTGGTGGCTGTCCGAGAGGCTGGGGGTCAGGTACTGAGTCTGCGTCTCCAGTCTGACCAAAATTCAATCTTTTTCTTGCAGGCTGCTGGCCCGCCTTTCCGGTTCCCGAGGAGGAGTCTGGCTCCACAGGAGAGTGCTCTACCGGCCTCTTTTTTCCCGGAGCCGTCTTAACAGGTTCCTCAACCAGGCCCAGAGGTTCAAGAACCCTCTTTTTCGCCTGGAAGACTGCTCGTCCGAGGTTGCCCCCAAAAGACGTATCTTCTTTAAGGCGCTCCTGGAACTCCGCGTCGGCGTGGTTGTACTTGAGGTAAGGGTTGTCTCCGCTGTCGAGCTGCCGGTCGTAGGCTTTGTCGTGCTCGAGGGCAGCGGCGTCTGCCTCGTTCACCGGCTCTCCCTTGTCCAGTCCGTTGAAGGGTCCGAGGTACTTGTACCCGGGAAGCACAAGGCCCCTGCTGTCGTCCTTATGCCGCTCTGCGGGCTTTGGTGGTGGTGGGCCGGGTTTGAGCTTCCACCACTGTCTTATTCCTTCAGAGAGAGTGTCCTCGAGCCAATCGGGTAGATAACCGTCGGCAGCCGTGGCGGCGCTAGCACCATGGCTCGAGATCCCGGGTGATCAAGTCTTCGTCGAGTGATTGTAAATAAAATGTAATTTACAGTATAGTATTTTAATTAATATACAAATGATTTGATAATAATTCTTATTTAACTATAATATATTGTGTTGGGTTGAATTAAAGGTCCGTATACGCCGCTACAGGGCGCGTACTATGGTTGCTTTGACGTATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCCCCTGCAGGCAGCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCGGGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCCATCACTAGGGGTTCCTACGCGTTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGT
ACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTCAGATCCGCTAGCGCTACCGGTCGCCACCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAGGTCGACGTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATCTAGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTAAAGTTTAAACAGGAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCGGCCTCAGTGAGCGAGCGAGCGCGCAGCTGCCTGCAGGACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCGCGGCCGCTCTAGAGATCATGGAGATAATTAAAATGATAACCATCTCGCAAATAAATAAGTATTTTACTGTTTTCGTAACAGTTTTGTAATAAAAAAACCTATAAATATTCCGGATTATTCATACCGTCCCACCATCGGGCGCGGATCCGCCGCCCTGGCGGGGTTTTACGAGATTGTGATTAAGGTCCCCAGCGACCTTGACGAGCATCTGCCCGGCATTTCTGACAGCTTTGTGAACTGGGTGGCCGAGAAGGAGTGGGAGTTGCCGCCAGATTCTGACTTGGATCTGAATCTGATTGAGCAGGCACCCCTGACCGTGGCCGAGAAGCTGCAGCGCGACTTTCTGACGGAGTGGCGCCGTGTGAGTAAGGCCCCGGAGGCCCTTTTCTTTGTGCAATTTGAGAAGGGAGAGAGCTACTTCCACTTACACGTGCTCGTGGAAACCACCGGGGTGAAATCCTTAGTTTTGGGACGTTTCCTGAGTCAGATTCGCGAAAAACTGATTCAGAGAATTTACCGCGGGATCGAGCCGACTTTGCCAAACTGGTTCGCGGTCACAAAGACCAGAAACGGCGCCGGAGGCGGGAACAAGGTGGTGGACGAGTGCTACATCCCCAATTACTTGCTCCCCAAAACCCAGCCTGAGCTCCAGTGGGCGTGGACTAATTTAGAACAGTATTTAAGCGCCTGTTTGAATCTCACGGAGCGTAAACGGTTGGTGGCGCAGCATCTGACGCACGTGTCGCAGACGCAGGAGCAGAACAAAGAGAATCAGAATCCCAATTCTGACGCGCCGGTGATCAGATCAAAAACTTCAGCCAGGTACATGGAGCTGGTCGGGTGGCTCGTGGACAAGGGGATTACCTCGGAGAAGCAGTGGATACAGGAGGACCAGGCCTCATACATCTCCTTCAATGCGGCCTCCAACTCGCGGTCCCAAATCAAGGCTGCCTTGGACAATGCGGGAAAGATTATGAGCCTGACTAAAACCGCCCCCGACTACCTGGTGGGCCAGCAGCCCGTGGAGGACATTTCCAGCAATCGGATTTATAAAATTTTGGAACTAAACGGGTACGATCCCCAATATGCGGCTTCCGTCTTTCTGGGATGGGCCACGAAAAAGTTCGGCAAGAGGAACACCATCTGGCTGTTTGGGCCTGCAACTACCGGGAAGACCAACATCGCGGAGGCCATAGCCCACACTGTGCCCTTCTACGGGTGCGTAAACTGGACCAATGAGAACTTTCCCTTCAACGACTGTGTGGACAAGATGGTGATCTGGTGGGAGGAGGGGAAGATGACCGCCAAGGTCGTGGAGTCGGCCAAAGCCATTCTCGGAGGAAGCAAGGTGCGCGTGGACCAGAAATGCAAGTCCTCGGCCCAGATAGACCCGACTCCCGTGATCGTCACCTCCAACACCAACATGTGCGCCGTGATTGACGGGAACTCAACGACCTTCGAACACCAGCAGCCGTTGCAAGACCGGATGTTCAAATTTGAACTCACCCGCCGTCTGGATCATGACTTTGGGAAGGTCACCAAGCAGGAAGTCAAAGACTTTTTCCGGTGGGCAAAGGATCACGTGGTTGAGGTGGAGCATGAATTCTACGTCAAAAAGGGTGGAGCCAAGAAAAGACCCGCCCCCAGTGACGCAGATATAAGTGAGCCCAAACGGGTGCGCGAGTCAGTTGCGCAGCCATCGACGTCAGACGCGGAAGCATCGATCAACTACGCAGACAGGTACCAAAACAAATGTTCTCGTCACGTGGGCATGAATCTGATGCTGTTTCCCTGCAGACAATGCGAGAGAATGAATCAGAATTCAAATATCTGCTTCACTCACGGACAGAAAGACTGTTTAGAGTGCTTTCCCGTGTCAGAATCTCAACCCGTTTCTGTCGTCAAAAAGGCGTATCAGAAACTGTGCTACATTCATCATATCATGGGAAAGGTGCCAGACGCTTGCACTGCCTGCGATCTGGTCAATGTGGATTTGGATGACTGCATCTTTGAACAATAATCTAGAGCCTGCAGTCTCGACAAGCTTGTCGAGAAGTACTAGAGGATCATAATCAGCCATACCACATTTGTAGAGGTTTTACTTGCTTTAAAAAACCTCCCACACCTCCCCCTGAACCTGAAACATAAAATGAATGCAATTGTTGTTGTTAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGGATCTGATCACTGCTTGAGCCTAGGAGATCCGAACCAGATAAGTGAAATCTAGTTCCAAACTATTTTGTCATTTTTAATTTTCGTATTAGCTTACGACGCTACACCCAGTTCCCATCTATTTTGTCACTCTTCCCTAAATAATCCTTAAAAA
CTCCATTTCCACCCCTCCCAGTTCCCAACTATTTTGTCCGCCCACAGCGGGGCATTTTTCTTCCTGTTATGTTTTTAATCAAACATCCTGCCAACTCCATGTGACAAACCGTCATCTTCGGCTACTTT
TTCTCTGTCACAGAATGAAAATTTTTCTGTCATCTCTTCGTTATTAATGTTTGTAATTGACTGAATATCAACGCTTATTTGCAGCCTGAATGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCAGACCAGCCGCGTAACCTGGCAAAATCGGTTACGGTTGAGTAATAAATGGATGCCCTGCGTAAGCGGGTGTGGGCGGACAATAAAGTCTTAAACTGAACAAAATAGATCTAAACTATGACAATAAAGTCTTAAACTAGACAGAATAGTTGTAAACTGAAATCAGTCCAGTTATGCTGTGAAAAAGCATACTGGACTTTTGTTATGGCTAAAGCAAACTCTTCATTTTCTGAAGTGCAAATTGCCCGTCGTATTAAAGAGGGGCGTGGCCAAGGGCATGGTAAAGACTATATTCGCGGCGTTGTGACAATTTACCGAACAACTCCGCGGCCGGGAAGCCGATCTCGGCTTGAACGAATTGTTAGGTGGCGGTACTTGGGTCGATATCAAAGTGCATCACTTCTTCCCGTATGCCCAACTTTGTATAGAGAGCCACTGCGGGATCGTCACCGTAATCTGCTTGCACGTAGATCACATAAGCACCAAGCGCGTTGGCCTCATGCTTGAGGAGATTGATGAGCGCGGTGGCAATGCCCTGCCTCCGGTGCTCGCCGGAGACTGCGAGATCATAGATATAGATCTCACTACGCGGCTGCTCAAACCTGGGCAGAACGTAAGCCGCGAGAGCGCCAACAACCGCTTCTTGGTCGAAGGCAGCAAGCGCGATGAATGTCTTACTACGGAGCAAGTTCCCGAGGTAATCGGAGTCCGGCTGATGTTGGGAGTAGGTGGCTACGTCTCCGAACTCACGACCGAAAAGATCAAGAGCAGCCCGCATGGATTTGACTTGGTCAGGGCCGAGCCTACATGTGCGAATGATGCCCATACTTGAGCCACCTAACTTTGTTTTAGGGCGACTGCCCTGCTGCGTAACATCGTTGCTGCTGCGTAACATCGTTGCTGCTCCATAACATCAAACATCGACCCACGGCGTAACGCGCTTGCTGCTTGGATGCCCGAGGCATAGACTGTACAAAAAAACAGTCATAACAAGCCATGAAAACCGCCACTGCGCCGTTACCACCGCTGCGTTCGGTCAAGGTTCTGGACCAGTTGCGTGAGCGCATACGCTACTTGCATTACAGTTTACGAACCGAACAGGCTTATGTCAACTGGGTTCGTGCCTTCATCCGTTTCCAC
GGTGTGCGTCACCCGGCAACCTTGGGCAGCAGCGAAGTCGAGGCATTTCTGTCCTGGCTGGCGAACGAGCGCAAGGTTTCGGTCTCCACGCATCGTCAGGCATTGGCGGCCTTGCTGTTCTTCTACGGCAAGGTGCTGTGCACGGATCTGCCCTGGCTTCAGGAGATCGGTAGACCTCGGCCGTCGCGGCGCTTGCCGGTGGTGCTGACCCCGGATGAAGTGGTTCGCATCCTCGGTTTTCTGGAAGGCGAGCATCGTTTGTTCGCCCAGGACTCTAGCTATAGTTCTAGTGGTTGGCCTACGTACCCGTAGTGGCTATGGCAGGGCTTGCCGCCCCGACGTTGGCTGCGAGCCCTGGGCCTTCACCCGAACTTGGGGGTTGGGGTGGGGAAAAGGAAGAAACGCGGGCGTATTGGTCCCAATGGGGTCTCGGTGGGGTATCGACAGAGTGCCAGCCCTGGGACCGAACCCCGCGTTTATGAACAAACGACCCAACACCCGTGCGTTTTATTCTGTCTTTTTATTGCCGTCATAGCGCGGGTTCCTTCCGGTATTGTCTCCTTCCGTGTTTCAGTTAGCCTCCCCCATCTCCCGGTACCGCATGCCTTAGTTGAACTCGAACTTCTTGTACTTGCAGTTGAGCTTTTGCTCGGCGAATGTGATGGCGTCGGAGAGTGGCACCAGTCCCTGCAGGATCAAGGCCAAGAGCTTCAGCAAGTTGTTGTGCAGTGTAGTTGACTCGCGACGGTTGACCTTGCCGATCACGAACATGTGGTGCTTGAATCTGTTGTACTTTTGGATGACCTGGCTCACATCAACGTCCTTGATTTCGCCAGAGATCCAGTAGAACTCCTTGTTCTTCTTAGCGATGGTCAGCCTTTCCTCGTTCTTGAAGGACAACACGATGAAGTTGTGACCCTTAACGTTATCGACGTTTTGAGTCAAGTACTGCTCAACGATGTGCATGCTTCCGTCTTCCTTCTTGACCTTCTTGAGGTTCTCGGCGTTGTTGTTAGAAGCGATGTTGTCGTGGTACTTGTAGTTGTTGAAGAGCAGGTTAGCGACGGACGAGTACTTGTATGTCAGAGTGCTCTTCTTGTTCACGATGAGGTTCAGGTTATCAACGACGTACTTGTTGGGAGGGTTGTCCGGGAATTGGACGGACTCCGAGTACTTGAGGATCTGGGAAACGTATGGCGAGACGAAGAAGTTGTTAGAGGCGGTTTCGATCTCGTTTGATTCCTTGCGGCTCAGCATGATGGGCAAAGTGAAGAGGTTCTTGTCCTGGTACATCTCGTACAACTTCGACAAGAGGAATCCACACTTACGCTCGCCGAGTGATTGCAGCAAGGTCACGAAGGTTGTCTGAGCGTAGTACATATCGAGGTTGAAGTAGGAGGTCAGAGCGGCCTTGAATGTGTGGTGGACGTCCACGAAGTGACACTTCTTGCAGTTTTGAGCGGCGGTCTCATCGTTGCAGACGTCCTGTGAGTGTGGGATTTCGATGCCAGTCTCCTTAACCAGGTTGTAGCTGATCATGAAGCGGATCTTATCGAAGGTCACAACGAACACACGGTTGTCAACCATGTAGTAGTTGTTTGTGTACTCGTAGACCACGTTAGAGACGTACTTGGCGAAGATGATTTCGAAAGGCTTCACCTCGGACTTCTTAACGACGAACATGTAGTAACCGGTTTCAGACATGTGATCGGAGAATCTGTTCGAGTTGTAGTCGTTATCGTCGAACCTCATCAGGTAGGGGGCGAAGTCGTTTGTGAAGTAGTGAGTGATCTCCTGGGTGGAAGCCACTGTACAGATGTTAGTGTTGTGGTTGATGGTTTGTTCCAGTGTAGCGCATGACTGGATGGTGCTCTTCTTGTACTTAGGTCTCAACTTGATCTTGTTGAATTGACCCACAACTCCCTGGGAGTTATCCAGGTACTCGTCCAACTTCCTCTTGGTGCCTGTGGCCGACGGCTGGTTGACACCAGCAGAGTGTTCGAAGGACTCGGCGTGGTAAGCTGAGCTAGGAGAGGGTTGTTCGACCACTGGCTGTTCGAGTGACTCGCTGTAGTAGGCAGAGGACACAGCTTCCTCCAGGTTGTCAGTGGTCTTGAGCAGACACTCCACCAAATCGTTGTCAGTGAGCGAGTTAACTGAGGCGAGGAAGTTGCTAGCGGCAGCGGTTTCAGAGTCGGAGATGACAGTATCAGCTCCGTCCGGGGTTGGGTGGTTGTAGTAAGACAAGTAATCGTTAGGTTGCTTGTCGCAGAACTCCGAGTATGAGTTGTCGAAGCTAGCACGAGAGGGAGTGCTGGCAGAGGTGTAGGAAGCGTTGAAGTTGATTTGAGTCATGGTGACCTGGTTGTTCACGATCTTATCGCCACCTGTGTCCACCTGCAGTTGCTGGGCCTCAGCGCAGGCTGAAGTGGCCTCACAGGAGTAGGGGCTGGAAGCACAGTTGGAAGTCATGATGTTTTCTTGGACGTTCAGGACGTGGCTGGATGTACGGATCATGCGCCCGATGGTGGGACGGTATGAATAATCCGGAATATTTATAGGTTTTTTTATTACAAAACTGTTACGAAAACAGTAAAATACTTATTTATTTGCGAGATGGTTATCATTTTAATTATCTCCATGATCGGATCTCCTAGGCTCAAGCAGTGATCAGATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTTGTCTAGTACTTCTCGACGGTACCGCATGCTATGCATCAGCTGCTAGCACCATGGCTCGAGTTACAGATTACGAGTCAGGTATCTGGTGCCAATGGGGCGAGGCTCTGAATACACGCCATTAGTGTCCACAGTAAAGTCCACATTAACAGACTTGTTGTAGTTGGAAGTGTACTGAATTTCGGGATTCCAGCGTTTGCTGTTTTCCTTCTGCAGCTCCCACTCGATCTCCACGCTGACCTGTCCCGTGGAGTACTGTGTGATGAAGGAAGCAAACTTTGCCGCACTGAAGGTGGTCGAAGGATTCGCAGGTACCGGGGTGTTCTTGATGAGAATCTGTGGAGGAGGGTGTTTAAGTCCGAATCCACCCATGAGGGGAGAGGGGTGAAAATGTCCGTCCGTGTGTGGAATCTTTGCCCAGATGGGCCCCTGAAGGTACACATCTCTGTCCTGCCAGACCATGCCTGGAAGAACGCCTTGTGTGTTGACATCTGCGGTAGCTGCTTGTCTGTTGCCTCTCTGGAGGTTGGTAGATACAGAACCATACTGCTCCGTAGCCACGGGATTGGTTGTCCTGATTTCCTCTTCGTCTGTAATCATGACCTTTTCAATGTCCACATTTGTTTTCTCTGAGCCTTGCTTCCCAAAGATGAGAACCCCGCTCTGAGGAAAAAACTTTTCTTCATCGTCCTTGTGGCTTGCCATCGCCGGGCCCGGATTCACCAGAGAGTCTCTGCCATTGAGGTGGTACTTGGTAGCTCCAGTCCACGAGTATTCACTGTTGTTGTTATCCGCAGATGTCTTTGATACTCGCTGCTGGCGGTAACAGGGTCCAGGAAGCCAGTTCCTAGACTGGTCCCGAATGTCACTCGCTCCGGCCTGAGAAAACTGAAGCCTTGACTGCGTGGTGGTTCCACTTGGAGTGTTTGTTCTGCTCAAGTAATACAGGTACTGGTCGATGAGAGGATTCATGAGACGGTCCAGACTCTGGCTGTGAGCGTAGCTGCTGTGGAAAGGAACGTCCTCAAAAGTGTAGCTGAAGGTAAAGTTGTTTCCGGTACGCAGCATCTGAGAAGGAAAGTACTCCAGGCAGTAAAATGAAGAGCGTCCTACTGCCTGACTCCCGTTGTTCAGGGTGAGGTATCCATACTGTGGCACCATGAAGACGTCTGCTGG
GAACGGCGGGAGGCATCCTTGATGCGCCGAGCCGAGGACGTACGGGAGCTGGTACTCCGAGTCAGTAAACACCTGAACCGTGCTGGTAAGGTTATTGGCAATCGTCGTCGTACCGTCATTCTGCGTGACCTCTTTGACTTGAATGTTAAAGAGCTTGAAGTTGAGTCTCTTGGGTCGGAATCCCCAGTTGTTGTTGATGAGTCTTTGCCAGTCACGTGGTGAAAAGTGGCAGTGGAATCTGTTGAAGTCAAAATACCCCCAAGGGGTGCTGTAGCCAAAGTAGTGATTGTCGTTCGAGGCTCCTGATTGGCTGGAAATTTGTTTGTAGAGGTGGTTGTTGTAGGTGGGCAGGGCCCAGGTTCGGGTGCTGGTGGTGATGACTCTGTCGCCCATCCATGTGGAATCGCAATGCCAATTTCCCGAGGAATTACCCACTCCGTCGGCGCCCTCGTTATTGTCTGCCATTGGTGCGCCACTGCCTGTAGCCATCGTATTAGTTCCCAGACCAGAGGGGGCTGCTGGTGGCTGTCCGAGAGGCTGGGGGTCAGGTACTGAGTCTGCGTCTCCAGTCTGACCAAAATTCAATCTTTTTCTTGCAGGCTGCTGGCCCGCCTTTCCGGTTCCCGAGGAGGAGTCTGGCTCCACAGGAGAGTGCTCTACCGGCCTCTTTTTTCCCGGAGCCGTCTTAACAGGTTCCTCAACCAGGCCCAGAGGTTCAAGAACCCTCTTTTTCGCCTGGAAGACTGCTCGTCCGAGGTTGCCCCCAAAAGACGTATCTTCTTTAAGGCGCTCCTGGAACTCCGCGTCGGCGTGGTTGTACTTGAGGTAAGGGTTGTCTCCGCTGTCGAGCTGCCGGTCGTAGGCTTTGTCGTGCTCGAGGGCAGCGGCGTCTGCCTCGTTCACCGGCTCTCCCTTGTCCAGTCCGTTGAAGGGTCCGAGGTACTTGTACCCGGGAAGCACAAGGCCCCTGCTGTCGTCCTTATGCCGCTCTGCGGGCTTTGGTGGTGGTGGGCCGGGTTTGAGCTTCCACCACTGTCTTATTCCTTCAGAGAGAGTGTCCTCGAGCCAATCGGGTAGATAACCGTCGGCAGCCGTGGCGGCTCAAGTCTTCGTCGAGTGATTGTAAATAAAATGTAATTTACAGTATAGTATTTTAATTAATATACAAATGATTTGATAATAATTCTTATTTAACTATAATATATTGTGTTGGGTTGAATTAAAGGTCCGTATCTCCAGACTGCAGTTTAAATTGTGTAATTTATGTAGCTGTAATTTTTACCTTATTAATATTTTTTACGCTTTGCATTCGACGACTGAACTCCCAAATATATGTTTAACTCGTCTTGGTCGTTTGAATTTTTGTTGCTGTGTTTCCTAATATTTTCCATCACCTTAAATATGTTATTGTAATCCTCAATGTTGAACTTGCAATTGGACACGGCATAGTTTTCCATAGTCGTGTAAAACATGGTATTGGCTGCATTGTAATACATCCGACTGAGCGGGTACGGATCTATGTGTTTGAGCAGCCTGTTCAAAAACTCTGCATCGTCGCAAAACGGAATTTGGTCACGATCCCGGTCCGAAGCGCGCGGAATTCAAAGCGCTACGTCAGCGACGTCATCGATTATTGCTCCAATACTAGGGTTTTGTTTTACTATCTGTTCTGCGGTCCACACATCTTCTTCGCTAGTCACGTACGCAAACAACTCGGGCCGCAAAGGTGTTTTAAAACATATCAGATTAGAATCTTTTATAACTTGGCCGCATTGTAAATAGTTGTGCCAACGCGCGGGAAACATTTTACACGTGCTTTCAATCTTATCTCATAAACGCAACTAAATATGATCCTTCAACTCGCTTTACGAGTAGAATTCTACTTGTAACGCACGATTAATTATGACTCATAAGCTGATGTCATGTTTTGCACACGGCTCATAACCGAACTCGCTTTACGAGTAGAATTCTACGCGTAAAACACAATCAAGTATGAGTCATAAGCTGATGTCATGTTTTGCACACGGCTCATAACCGAACTGGCTTTACGAGTAGAATTCTACTTGTAACGCACGATCAGTGGATGATGTCATAAACTTATCTCATGTTTTGCACACGGCTCATAACTTGAGTCATAAGCTGATATCAGAAGATTATTAACGTGTTTAGGTATGACTCATTTGTTTTCAAAACTGAACTCGCTTTACGGATAGAATTCTACTTGTAAAACACAATCAGGGGATGATGTCATTATACAAATGATGTCATTTGTTTTTCAAAACTAAACTCGCTTTACGGGTAGAATTCTACTTGTAAAACACAATCGAGGGATAATGTCATTTGTTATTCTCAAATATGATGTCATTTAAATTTTTATTATGCAAGAATTGTATGTTGCTGTGTCATTTATGAATGTTTTATCGACAACTTGTTTATTTGTTTCATTGACTTGTTGTTGTTCGTGATTATGATTAATTATAAATCTTTTGTTGGGGTCAACATCGATGTATACGCCGCTACAGGGCGCGTACTATGGTTGCTTTGACGTATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCCCCTGCAGGCAGCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCGGGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCCATCACTAGGGGTTCCTACGCGTTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTCAGATCCGCTAGCGCTACCGGTCGCCACCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAA
CGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAGGTCGACGTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATCTAGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTAAAGTTTAAACAGGAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCGGCCTCAGTGAGCGAGCGAGCGCGCAGCTGCCTGCAGGACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCGCGGCCGCTCTAGAGATCATGGAGATAATTAAAATGATAACCATCTCGCAAATAAATAAGTATTTTACTGTTTTCGTAACAGTTTTGTAATAAAAAAACCTATAAATATTCCGGATTATTCATACCGTCCCACCATCGGGCGCGGATCCGCCGCCCTGGCGGGGTTTTACGAGATTGTGATTAAGGTCCCCAGCGACCTTGACGAGCATCTGCCCGGCATTTCTGACAGCTTTGTGAACTGGGTGGCCGAGAAGGAGTGGGAGTTGCCGCCAGATTCTGACTTGGATCTGAATCTGATTGAGCAGGCACCCCTGACCGTGGCCGAGAAGCTGCAGCGCGACTTTCTGACGGAGTGGCGCCGTGTGAGTAAGGCCCCGGAGGCCCTTTTCTTTGTGCAATTTGAGAAGGGAGAGAGCTACTTCCACTTACACGTGCTCGTGGAAACCACCGGGGTGAAATCCTTAGTTTTGGGACGTTTCCTGAGTCAGATTCGCGAAAAACTGATTCAGAGAATTTACCGCGGGATCGAGCCGACTTTGCCAAACTGGTTCGCGGTCACAAAGACCAGAAACGGCGCCGGAGGCGGGAACAAGGTGGTGGACGAGTGCTACATCCCCAATTACTTGCTCCCCAAAACCCAGCCTGAGCTCCAGTGGGCGTGGACTAATTTAGAACAGTATTTAAGCGCCTGTTTGAATCTCACGGAGCGTAAACGGTTGGTGGCGCAGCATCTGACGCACGTGTCGCAGACGCAGGAGCAGAACAAAGAGAATCAGAATCCCAATTCTGACGCGCCGGTGATCAGATCAAAAACTTCAGCCAGGTACATGGAGCTGGTCGGGTGGCTCGTGGACAAGGGGATTACCTCGGAGAAGCAGTGGATACAGGAGGACCAGGCCTCATACATCTCCTTCAATGCGGCCTCCAACTCGCGGTCCCAAATCAAGGCTGCCTTGGACAATGCGGGAAAGATTATGAGCCTGACTAAAACCGCCCCCGACTACCTGGTGGGCCAGCAGCCCGTGGAGGACATTTCCAGCAATCGGATTTATAAAATTTTGGAACTAAACGGGTACGATCCCCAATATGCGGCTTCCGTCTTTCTGGGATGGGCCACGAAAAAGTTCGGCAAGAGGAACACCATCTGGCTGTTTGGGCCTGCAACTACCGGGAAGACCAACATCGCGGAGGCCATAGCCCACACTGTGCCCTTCTACGGGTGCGTAAACTGGACCAATGAGAACTTTCCCTTCAACGACTGTGTGGACAAGATGGTGATCTGGTGGGAGGAGGGGAAGATGACCGCCAAGGTCGTGGAGTCGGCCAAAGCCATTCTCGGAGGAAGCAAGGTGCGCGTGGACCAGAAATGCAAGTCCTCGGCCCAGATAGACCCGACTCCCGTGATCGTCACCTCCAACACCAACATGTGCGCCGTGATTGACGGGAACTCAACGACCTTCGAACACCAGCAGCCGTTGCAAGACCGGATGTTCAAATTTGAACTCACCCGCCGTCTGGATCATGACTTTGGGAAGGTCACCAAGCAGGAAGTCAAAGACTTTTTCCGGTGGGCAAAGGATCACGTGGTTGAGGTGGAGCATGAATTCTACGTCAAAAAGGGTGGAGCCAAGAAAAGACCCGCCCCCAGTGACGCAGATATAAGTGAGCCCAAACGGGTGCGCGAGTCAGTTGCGCAGCCATCGACGTCAGACGCGGAAGCATCGATCAACTACGCAGACAGGTACCAAAACAAATGTTCTCGTCACGTGGGCATGAATCTGATGCTGTTTCCCTGCAGACAATGCGAGAGAATGAATCAGAATTCAAATATCTGCTTCACTCACGGACAGAAAGACTGTTTAGAGTGCTTTCCCGTGTCAGAATCTCAACCCGTTTCTGTCGTCAAAAAGGCGTATCAGAAACTGTGCTACATTCATCATATCATGGGAAAGGTGCCAGACGCTTGCACTGCCTGCGATCTGGTCAATGTGGATTTGGATGACTGCATCTTTGAACAATAATCTAGAGCCTGCAGTCTCGACAAGCTTGTCGAGAAGTACTAGAGGATCATAATCAGCCATACCACATTTGTAGAGGTTTTACTTGCTTTAAAAAACCTCCCACACCTCCCCCTGAACCTGAAACATAAAATGAATGCAATTGTTGTTGTTAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGGATCTGATCACTGCTTGAGCCTAGGAGATCCGAACCAGATAAGTGAAATCTAGTTCCAAACTATTTTGTCATTTTTAATTTTCGTATTAGCTTACGACGCTACACCCAGTTCCCATCTATTTTGTCACTCTTCCCTAAATAATCCTTAAAAACTCCATTTCCACCCCTCCCAGTTCCCAACTATTTTGTCCGCCCACAGCGGGGCATTTTTCTTCCTGTTATGTTTTTAATCAAACATCCTGCCAACTCCATGTGACAAACCGTCATCTTCGGCTACTTT
い。
2種類のP2継代組換えバキュロウイルスをMOI=0.1で25mLの懸濁SF9細胞(密度3.0E+06細胞/mL)にそれぞれ感染させ、4日後に細胞を回収し、5mLのTNT溶解液(20mM Tris-HCl (pH7.5)、150mM NaCl、1% Triton(登録商標) X-100、10mmol/l MgCl2)を用いて室温で1時間インキュベートして細胞を溶解した。最終濃度50U/mLのヌクレアーゼを追加して37℃で2時間消化し、サンプルを取り、プロテイナーゼK(TIANGEN)を用いて56℃で外殻を1時間消化し、qPCRで力価を測定した。検出手順は以下のとおりである。
ACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATAAAATTGTAAACGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGCCCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCAAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTACTATGGTTGCTTTGACGTATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCCCCTGCAGGCAGCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCG
GGCAAAGCCCGGGCGTCGGGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCCATCACTAGGGGTTCCTACGCGTTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTCAGATCCGCTAGCGCTACCGGTCGCCACCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAGGTCGACGTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATCTAGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTAAAGTTTAAACAGGAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCGGCCTCAGTGAGCGAGCGAGCGCGCAGCTGCCTGCAGG
ITR Forward primer 5’-GGAACCCCTAGTGATGGAGTT-3’(配列番号2)
ITR Reverse primer 5’-CGGCCTCAGTGAGCGA-3’(配列番号3)
感染48時間後、72時間後、96時間後、それぞれ倒立蛍光顕微鏡下で2種類のバキュロウイルスに感染した細胞を観察したところ、最適化された組換えバキュロウイルスは標的遺伝子(本実施例の標的遺伝子はEGFPである)の発現を大幅に減少させることができた(図5)。標的遺伝子(EGFP)の発現量の減少は、細胞内のrAAVパッケージングへの干渉を弱めるようであり、rAAVパッケージング関連の構造及び機能タンパク質の発現により有利である。
POROS(商標) CaptureSelect(登録商標)アフィニティークロマトグラフィー充填剤を用いて細胞溶解液を精製し、蛍光定量PCR法により力価を測定し(測定方法は実施例2と同様)、精製したrAAV2をネガティブ染色処理し、透過型電子顕微鏡下で観察した(図7)。ゲノムをパッケージングしたrAAV2は中実粒子であり、核酸を含まない欠陥のあるrAAV粒子の中心は暗く染色された。全体として、形状は損なわれておらず、従来方法(非特許文献1)と比較して空カプシド率が大幅に低下し(約3%)、これは、最適化されたバキュロウイルスベクター構築物を用いると、パッケージング製品中の空カプシドウイルスの割合が大幅に減少することを示し、下流の空カプシドウイルス除去などの工程の負担が大幅に軽減される。
Claims (17)
- 核酸構築物であって、
AAVエレメント、及びIEタンパク質をコードするポリヌクレオチドを含み、
前記AAVエレメントがCapタンパク質をコードするポリヌクレオチド、Repタンパク質をコードするポリヌクレオチド、及びAAVシスエレメントを含むことを特徴とする、
核酸構築物。 - バキュロウイルス組換え相同領域をコードするポリヌクレオチドを更に含むことを特徴とする、請求項1に記載の核酸構築物。
- 前記IEタンパク質が、Acie0、Acie01又はAcie02遺伝子のうちの1つ以上によってコードされ、及び/又は、前記バキュロウイルス組換え相同領域が、hr1、hr2、hr3、hr4又はhr5のうちの1つ以上から選択されることを特徴とする、請求項2に記載の核酸構築物。
- 前記核酸構築物がIEタンパク質遺伝子のプロモーターを更に含み、前記IEタンパク質遺伝子のプロモーターがGp64、pH、p6.9又はp10のうちの1つ以上から選択されることを特徴とする、請求項1に記載の核酸構築物。
- 前記核酸構築物がバキュロウイルスプロモーターを更に含み、前記バキュロウイルスプロモーターがバキュロウイルス組換え相同領域に結合されており、前記バキュロウイルスプロモーターが好ましくはpH、Gp64、p6.9又はp10のうちの1つ以上であることを特徴とする、請求項1に記載の核酸構築物。
- 前記AAVシスエレメントがITR配列から選択されることを特徴とする、請求項1に記載の核酸構築物。
- 前記核酸構築物が外来標的遺伝子を更に含み、前記外来標的遺伝子がAAVエレメントに埋め込まれていることを特徴とする、請求項1に記載の核酸構築物。
- 前記核酸構築物の構造が、IE遺伝子発現カセット-Cap遺伝子発現カセット-ITR-外来標的遺伝子発現カセット-ITR-Rep遺伝子発現カセットであることを特徴とする、請求項7に記載の核酸構築物。
- 前記核酸構築物のヌクレオチド配列が配列番号1であることを特徴とする、請求項1に記載の核酸構築物。
- 前記核酸構築物がアデノ随伴ウイルスベクター又は組換えバキュロウイルスベクターであり、前記組換えバキュロウイルスベクターが好ましくは組換えバキュロウイルスシャトルベクターであることを特徴とする、請求項1に記載の核酸構築物。
- 請求項1~10のいずれか一項に記載の核酸構築物をバキュロウイルス系によって構築することによって得られる、又は請求項1~10のいずれか一項に記載の核酸構築物中の任意のエレメントを含む核酸構築物をバキュロウイルス系によって共同構築することによって得られることを特徴とする、組換えバキュロウイルス。
- 請求項11に記載の組換えバキュロウイルスを細胞に感染させた後にパッケージングすることによって得られることを特徴とする、アデノ随伴ウイルス。
- 請求項11に記載の組換えバキュロウイルスに感染した細胞株であることを特徴とする、細胞株。
- バキュロウイルス系及び請求項1~10のいずれか一項に記載の核酸構築物を含むことを特徴とする、アデノ随伴ウイルスベクター系。
- 外来標的遺伝子を運ぶAAVエレメント、IEタンパク質をコードするポリヌクレオチド、及びバキュロウイルス組換え相同領域をコードするポリヌクレオチドをバキュロウイルスベクター骨格に組み込むことを含むことを特徴とする、請求項1~10のいずれか一項に記載の核酸構築物を構築する方法。
- 前記構築方法が以下の特徴のうちの1つ以上を含むことを特徴とする、請求項15に記載の構築方法。
1)前記AAVエレメントは、Capタンパク質をコードするポリヌクレオチド、Repタンパク質をコードするポリヌクレオチド、及びAAVシスエレメントを含み、前記AAVシスエレメントは、好ましくはITR配列である。
2)IEタンパク質をコードする前記ポリヌクレオチドは、Acie0、Acie01又はAcie02のうちの1つ以上から選択される。
3)前記バキュロウイルスベクター骨格は、pFastBacdual、pFastBac1、pFastBacHTA、pFastBacHTB又はpFastBacHTCのうちの1つから選択される。
4)前記バキュロウイルス組換え相同領域は、hr1、hr2、hr3、hr4又はhr5のうちの1つ以上から選択される。 - 請求項11に記載の組換えバキュロウイルスを昆虫細胞株に感染させる工程を含むことを特徴とする、アデノ随伴ウイルスの産生方法。
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