JP2023528476A - 組み換え微生物においてゲノムに安定に組み込むための方法 - Google Patents
組み換え微生物においてゲノムに安定に組み込むための方法 Download PDFInfo
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- JP2023528476A JP2023528476A JP2022574571A JP2022574571A JP2023528476A JP 2023528476 A JP2023528476 A JP 2023528476A JP 2022574571 A JP2022574571 A JP 2022574571A JP 2022574571 A JP2022574571 A JP 2022574571A JP 2023528476 A JP2023528476 A JP 2023528476A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/111—General methods applicable to biologically active non-coding nucleic acids
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
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- C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6402—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals
- C12N9/6405—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals not being snakes
- C12N9/6408—Serine endopeptidases (3.4.21)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/44—Staphylococcus
- C12R2001/445—Staphylococcus aureus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21026—Prolyl oligopeptidase (3.4.21.26), i.e. proline-specific endopeptidase
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- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Mycology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicinal Preparation (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202063033681P | 2020-06-02 | 2020-06-02 | |
| US63/033,681 | 2020-06-02 | ||
| US202063049544P | 2020-07-08 | 2020-07-08 | |
| US63/049,544 | 2020-07-08 | ||
| PCT/US2021/035484 WO2021247729A1 (en) | 2020-06-02 | 2021-06-02 | Methods for stable genomic integration in recombinant microorganisms |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2023528476A true JP2023528476A (ja) | 2023-07-04 |
| JP2023528476A5 JP2023528476A5 (https=) | 2024-06-11 |
Family
ID=78829905
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022574571A Pending JP2023528476A (ja) | 2020-06-02 | 2021-06-02 | 組み換え微生物においてゲノムに安定に組み込むための方法 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20220025364A1 (https=) |
| EP (1) | EP4158010A4 (https=) |
| JP (1) | JP2023528476A (https=) |
| CA (1) | CA3181274A1 (https=) |
| MX (1) | MX2022015299A (https=) |
| WO (1) | WO2021247729A1 (https=) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114015622A (zh) * | 2021-12-14 | 2022-02-08 | 塔里木大学 | 无乳链球菌培养基及其制备方法 |
| WO2024072985A1 (en) * | 2022-09-28 | 2024-04-04 | University Of Tennessee Research Foundation | Polyvalent vaccines for staphylococcal & streptococcal infection |
| CN118858626B (zh) * | 2024-09-20 | 2025-01-03 | 吉林大学 | 一种用于检测血浆金黄色葡萄球菌抗体的检测试剂、试剂盒及方法 |
| CN119162219B (zh) * | 2024-09-24 | 2025-08-12 | 湖北大学 | 过表达perR基因在提高苏云金芽胞杆菌生物锰氧化作用中的应用 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100190160A1 (en) * | 2007-01-24 | 2010-07-29 | Shriners Hospitals For Children | Indicator cell lines and methods for making same |
| WO2019113096A1 (en) * | 2017-12-05 | 2019-06-13 | BioPlx, Inc. | Methods and compositions to prevent microbial infection |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2003269379A1 (en) * | 2002-10-31 | 2004-05-25 | Pfizer Products Inc. | Suspension method for producing embryoid bodies, and compositions and methods related thereto |
| EP1524320A1 (en) * | 2003-10-15 | 2005-04-20 | Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts | Rapid determination and quantification of mycoplasma contamination using DNA chip technology |
| US20060260004A1 (en) * | 2004-04-01 | 2006-11-16 | Yiwen Fang | Par-related protein promoters |
| US7674621B2 (en) * | 2004-05-21 | 2010-03-09 | The United States Of America As Represented By The Department Of Health And Human Services | Plasmids and phages for homologous recombination and methods of use |
| MX2007010008A (es) * | 2005-02-16 | 2008-01-18 | Lentigen Corp | Vectores lentivirales y su uso. |
| CN101300356A (zh) * | 2005-09-09 | 2008-11-05 | 基因组股份公司 | 用于琥珀酸盐之生长关联性生产的方法和生物 |
| KR20090023464A (ko) * | 2006-06-08 | 2009-03-04 | 가부시키가이샤 아미노 압 가가쿠 | 유전자 산물의 생산량을 제어하는 방법 및 생산량 제어제 |
| WO2013016427A1 (en) * | 2011-07-25 | 2013-01-31 | Alvine Pharmaceuticals, Inc. | Methods and pharmaceutical compositions for treating celiac disease and gluten intolerance |
| EP3355939A4 (en) * | 2015-09-30 | 2019-04-17 | Trustees of Boston University | MICROBIAL DEADMAN AND PASS CODE EMERGENCY STOP SWITCHES |
| WO2017123676A1 (en) * | 2016-01-11 | 2017-07-20 | Synlogic, Inc. | Recombinant bacteria engineered to treat diseases and disorders associated with amino acid metabolism and methods of use thereof |
| CA3146363A1 (en) * | 2019-07-08 | 2021-01-14 | BioPlx, Inc. | Live biotherapeutic compositions and methods |
-
2021
- 2021-06-02 US US17/337,033 patent/US20220025364A1/en active Pending
- 2021-06-02 WO PCT/US2021/035484 patent/WO2021247729A1/en not_active Ceased
- 2021-06-02 CA CA3181274A patent/CA3181274A1/en active Pending
- 2021-06-02 JP JP2022574571A patent/JP2023528476A/ja active Pending
- 2021-06-02 EP EP21818391.1A patent/EP4158010A4/en not_active Withdrawn
- 2021-06-02 MX MX2022015299A patent/MX2022015299A/es unknown
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100190160A1 (en) * | 2007-01-24 | 2010-07-29 | Shriners Hospitals For Children | Indicator cell lines and methods for making same |
| WO2019113096A1 (en) * | 2017-12-05 | 2019-06-13 | BioPlx, Inc. | Methods and compositions to prevent microbial infection |
Also Published As
| Publication number | Publication date |
|---|---|
| MX2022015299A (es) | 2023-03-13 |
| EP4158010A1 (en) | 2023-04-05 |
| CA3181274A1 (en) | 2021-12-09 |
| EP4158010A4 (en) | 2025-02-19 |
| WO2021247729A1 (en) | 2021-12-09 |
| US20220025364A1 (en) | 2022-01-27 |
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