JP2023528476A - 組み換え微生物においてゲノムに安定に組み込むための方法 - Google Patents

組み換え微生物においてゲノムに安定に組み込むための方法 Download PDF

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JP2023528476A
JP2023528476A JP2022574571A JP2022574571A JP2023528476A JP 2023528476 A JP2023528476 A JP 2023528476A JP 2022574571 A JP2022574571 A JP 2022574571A JP 2022574571 A JP2022574571 A JP 2022574571A JP 2023528476 A JP2023528476 A JP 2023528476A
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microorganism
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ラウス ダン
ダブリュ.スターズル ティモシー
エス.ブイ.スターズル ラビ
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バイオピーエルエックス,インコーポレイティド
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    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6402Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals
    • C12N9/6405Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals not being snakes
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    • C12Y304/21026Prolyl oligopeptidase (3.4.21.26), i.e. proline-specific endopeptidase

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JP2022574571A 2020-06-02 2021-06-02 組み換え微生物においてゲノムに安定に組み込むための方法 Pending JP2023528476A (ja)

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US202063033681P 2020-06-02 2020-06-02
US63/033,681 2020-06-02
US202063049544P 2020-07-08 2020-07-08
US63/049,544 2020-07-08
PCT/US2021/035484 WO2021247729A1 (en) 2020-06-02 2021-06-02 Methods for stable genomic integration in recombinant microorganisms

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JP2023528476A true JP2023528476A (ja) 2023-07-04
JP2023528476A5 JP2023528476A5 (https=) 2024-06-11

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EP (1) EP4158010A4 (https=)
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CA (1) CA3181274A1 (https=)
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CN114015622A (zh) * 2021-12-14 2022-02-08 塔里木大学 无乳链球菌培养基及其制备方法
WO2024072985A1 (en) * 2022-09-28 2024-04-04 University Of Tennessee Research Foundation Polyvalent vaccines for staphylococcal & streptococcal infection
CN118858626B (zh) * 2024-09-20 2025-01-03 吉林大学 一种用于检测血浆金黄色葡萄球菌抗体的检测试剂、试剂盒及方法
CN119162219B (zh) * 2024-09-24 2025-08-12 湖北大学 过表达perR基因在提高苏云金芽胞杆菌生物锰氧化作用中的应用

Citations (2)

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US20100190160A1 (en) * 2007-01-24 2010-07-29 Shriners Hospitals For Children Indicator cell lines and methods for making same
WO2019113096A1 (en) * 2017-12-05 2019-06-13 BioPlx, Inc. Methods and compositions to prevent microbial infection

Family Cites Families (11)

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Publication number Priority date Publication date Assignee Title
AU2003269379A1 (en) * 2002-10-31 2004-05-25 Pfizer Products Inc. Suspension method for producing embryoid bodies, and compositions and methods related thereto
EP1524320A1 (en) * 2003-10-15 2005-04-20 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Rapid determination and quantification of mycoplasma contamination using DNA chip technology
US20060260004A1 (en) * 2004-04-01 2006-11-16 Yiwen Fang Par-related protein promoters
US7674621B2 (en) * 2004-05-21 2010-03-09 The United States Of America As Represented By The Department Of Health And Human Services Plasmids and phages for homologous recombination and methods of use
MX2007010008A (es) * 2005-02-16 2008-01-18 Lentigen Corp Vectores lentivirales y su uso.
CN101300356A (zh) * 2005-09-09 2008-11-05 基因组股份公司 用于琥珀酸盐之生长关联性生产的方法和生物
KR20090023464A (ko) * 2006-06-08 2009-03-04 가부시키가이샤 아미노 압 가가쿠 유전자 산물의 생산량을 제어하는 방법 및 생산량 제어제
WO2013016427A1 (en) * 2011-07-25 2013-01-31 Alvine Pharmaceuticals, Inc. Methods and pharmaceutical compositions for treating celiac disease and gluten intolerance
EP3355939A4 (en) * 2015-09-30 2019-04-17 Trustees of Boston University MICROBIAL DEADMAN AND PASS CODE EMERGENCY STOP SWITCHES
WO2017123676A1 (en) * 2016-01-11 2017-07-20 Synlogic, Inc. Recombinant bacteria engineered to treat diseases and disorders associated with amino acid metabolism and methods of use thereof
CA3146363A1 (en) * 2019-07-08 2021-01-14 BioPlx, Inc. Live biotherapeutic compositions and methods

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100190160A1 (en) * 2007-01-24 2010-07-29 Shriners Hospitals For Children Indicator cell lines and methods for making same
WO2019113096A1 (en) * 2017-12-05 2019-06-13 BioPlx, Inc. Methods and compositions to prevent microbial infection

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MX2022015299A (es) 2023-03-13
EP4158010A1 (en) 2023-04-05
CA3181274A1 (en) 2021-12-09
EP4158010A4 (en) 2025-02-19
WO2021247729A1 (en) 2021-12-09
US20220025364A1 (en) 2022-01-27

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