JP2023520213A - 隣接した腸単層システムに於いて腸内分泌細胞及び其れ等のサブタイプを富化する方法 - Google Patents

隣接した腸単層システムに於いて腸内分泌細胞及び其れ等のサブタイプを富化する方法 Download PDF

Info

Publication number
JP2023520213A
JP2023520213A JP2022559825A JP2022559825A JP2023520213A JP 2023520213 A JP2023520213 A JP 2023520213A JP 2022559825 A JP2022559825 A JP 2022559825A JP 2022559825 A JP2022559825 A JP 2022559825A JP 2023520213 A JP2023520213 A JP 2023520213A
Authority
JP
Japan
Prior art keywords
cells
eec
cell
compounds
culture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2022559825A
Other languages
English (en)
Japanese (ja)
Other versions
JP2023520213A5 (https=
Inventor
アルブリトン，ナンシー，エル．
ワン，ユリ
シムズ，クリストファー，イー．
ノボア，セシリア ヴィレガス
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Washington
University of North Carolina at Chapel Hill
Original Assignee
University of Washington
University of North Carolina at Chapel Hill
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Washington, University of North Carolina at Chapel Hill filed Critical University of Washington
Publication of JP2023520213A publication Critical patent/JP2023520213A/ja
Publication of JP2023520213A5 publication Critical patent/JP2023520213A5/ja
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/08—Chemical, biochemical or biological means, e.g. plasma jet, co-culture
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/02—Membranes; Filters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/02—Membranes; Filters
    • C12M25/04—Membranes; Filters in combination with well or multiwell plates, i.e. culture inserts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0679—Cells of the gastro-intestinal tract
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C12N2503/02—Drug screening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Physiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2022559825A 2020-04-03 2021-04-05 隣接した腸単層システムに於いて腸内分泌細胞及び其れ等のサブタイプを富化する方法 Pending JP2023520213A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063004537P 2020-04-03 2020-04-03
US63/004,537 2020-04-03
PCT/US2021/025741 WO2021203087A1 (en) 2020-04-03 2021-04-05 Methods to enrich enteroendocrine cells and their subtypes in the contiguous, intestinal monolayer systems

Publications (2)

Publication Number Publication Date
JP2023520213A true JP2023520213A (ja) 2023-05-16
JP2023520213A5 JP2023520213A5 (https=) 2025-11-12

Family

ID=77927600

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2022559825A Pending JP2023520213A (ja) 2020-04-03 2021-04-05 隣接した腸単層システムに於いて腸内分泌細胞及び其れ等のサブタイプを富化する方法

Country Status (5)

Country Link
US (1) US20230147744A1 (https=)
EP (1) EP4126218A4 (https=)
JP (1) JP2023520213A (https=)
CA (1) CA3170294A1 (https=)
WO (1) WO2021203087A1 (https=)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009250977A (ja) * 2008-04-07 2009-10-29 Arena Pharmaceuticals Inc ペプチドｙｙ（ｐｙｙ）分泌促進因子およびｐｙｙによってモジュレートされる状態の処置に有用な化合物を同定するためにｇタンパク質共役レセプターを使用する方法
WO2019141824A1 (en) * 2018-01-18 2019-07-25 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Generation, proliferation and expansion of epithelial cells from primary tissue into mucosoid cultures

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9464275B2 (en) * 2008-08-21 2016-10-11 The Board Of Trustees Of The Leland Stanford Junior University Ex vivo culture, proliferation and expansion of intestinal epithelium
US11193110B2 (en) * 2015-01-30 2021-12-07 The University Of North Carolina At Chapel Hill Methods to generate gastrointestinal epithelial tissue constructs
CA3093585A1 (en) * 2018-05-25 2019-11-28 The University Of North Carolina At Chapel Hill Formation of arrays of planar intestinal crypts possessing a stem/proliferattve cell compartment and differentiated cell zone
EP3880785A4 (en) * 2018-11-16 2022-08-24 The University of North Carolina at Chapel Hill In vitro cell culture mucus systems

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009250977A (ja) * 2008-04-07 2009-10-29 Arena Pharmaceuticals Inc ペプチドｙｙ（ｐｙｙ）分泌促進因子およびｐｙｙによってモジュレートされる状態の処置に有用な化合物を同定するためにｇタンパク質共役レセプターを使用する方法
WO2019141824A1 (en) * 2018-01-18 2019-07-25 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Generation, proliferation and expansion of epithelial cells from primary tissue into mucosoid cultures

Also Published As

Publication number Publication date
EP4126218A4 (en) 2024-07-10
US20230147744A1 (en) 2023-05-11
EP4126218A1 (en) 2023-02-08
WO2021203087A1 (en) 2021-10-07
CA3170294A1 (en) 2021-10-07

Similar Documents

Publication Publication Date Title
Yao et al. Organoids: development and applications in disease models, drug discovery, precision medicine, and regenerative medicine
AU2020200515B2 (en) Culture media for stem cells
Chandra et al. Derivation of adult canine intestinal organoids for translational research in gastroenterology
JP7323932B2 (ja) 共通の培地を活用する、多臓器「生体機能チップ」装置
JP5875517B2 (ja) 膵ホルモン産生細胞の製造法
US20260049268A1 (en) In vitro cell culture mucus systems
McCoy et al. In vitro models for investigating intestinal host–pathogen interactions
JP7300986B2 (ja) 人工腸組織およびその使用
KR20180108789A (ko) 간 섬유화의 모델 시스템 및 그의 제조 및 사용 방법
EP3223831A1 (en) Methods for generation of podocytes from pluripotent stem cells and cells produced by the same
CN105358677A (zh) 用于上皮干细胞扩增和培养的组合物和方法
CN110352239A (zh) 可筛选的肝脏疾病模型和方法
Zhao et al. Cartilage-on-chip for osteoarthritis drug screening
Cao et al. Self‐assembled human placental model from trophoblast stem cells in a dynamic organ‐on‐a‐chip system
Orbach et al. In vitro intestinal and liver models for toxicity testing
US20200362312A1 (en) Generation, proliferation and expansion of epithelial cells from primary tissue into mucosoid cultures
CN109563478A (zh) 多能干细胞分化成肠中肠内胚层细胞
Jones et al. Paediatric gastric organoids as a tool for disease modelling and clinical translation
Park et al. Three-dimensional organoids as a model to study nonalcoholic fatty liver disease
JP2023520213A (ja) 隣接した腸単層システムに於いて腸内分泌細胞及び其れ等のサブタイプを富化する方法
Kim et al. A convective transport-enhanced multi-organoid device for therapeutic modeling of the liver-pancreas axis in obesity
US20210087533A1 (en) Canine epithelial organoids and methods of making, recovering, and use
EP4495227A1 (en) Co-cultures of organoids and stromal cells
Qi et al. Centimeter-scale, physiologically relevant intestinal organoids generated entirely from pluripotent stem cells
Chen et al. Populational analysis of suspended microtissues for high-throughput, multiplexed 3D tissue engineering

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20240308

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20240308

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20250331

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20250401

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20250627

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20250805

A524 Written submission of copy of amendment under article 19 pct

Free format text: JAPANESE INTERMEDIATE CODE: A524

Effective date: 20251031

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20251209

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20260408

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20260408