JP2023514717A - エムトール信号伝達抑制剤を有効成分として含む癌の予防又は治療用薬剤学的組成物 - Google Patents
エムトール信号伝達抑制剤を有効成分として含む癌の予防又は治療用薬剤学的組成物 Download PDFInfo
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Abstract
Description
本発明は、エムトール(mTOR)信号伝達抑制剤を有効成分として含む癌の予防又は治療用薬剤学的組成物を提供する。
NCM460細胞(ヒト結腸正常細胞)、HCT116細胞(ヒト結腸癌細胞、p53野生型)、HT29細胞(ヒト結腸癌細胞、p53突然変異体)、SW480細胞(ヒト結腸癌細胞、p53突然変異体)、MDA-MB-231細胞(ヒト乳癌細胞)、MDA-MB-468細胞(ヒト乳癌細胞)、HS746T細胞(ヒト胃癌細胞)、SNU1細胞(ヒト胃癌細胞)、SNU2細胞(ヒト胃癌細胞)、MC38細胞(ネズミ大腸癌細胞)、B16F10細胞(ネズミ黒色腫細胞)、をATCC(American Type Culture Collection,Virginia,USA)から購入して使用した。
<実施例2-1.ロミタピドが処理された大腸癌細胞の生存力分析>
NCM460、HCT116、HT29、SW480細胞を96ウェルプレートに104細胞/ウェルの密度で接種し、24時間、37℃で培養した後、様々な濃度(0、1、2、5、10μM)のロミタピド(Sigma Aldrich,Missouri,USA)で処理した。ロミタピド処理後にプレートを5% CO2、37℃で24時間培養した。その後、細胞を、分析試薬(CellTiter-Glo(登録商標) Reagent)100μLを各ウェルに添加した後、VICTOR X Multilabel Reader(PerkinElmer,Massachusetts,USA)を用いて発光(luminescence)を測定し、これによって細胞の生存率(%)を計算した。結果は図2~図4に示した。
大腸癌細胞以外の様々な120種の癌細胞に対して様々な濃度のロミタピドを処理し、細胞生存力を分析した。
<実施例3-1.ロミタピドが処理された大腸癌細胞及び乳癌細胞の信号伝達分析>
HCT116、SW480、HT29、MDA-MB-231、MDA-MB-468細胞株でのロミタピドの機能を確認するために、ロミタピド添加時における細胞の信号伝達効果を調べてみた。
HS746T、SNU1、SNU2細胞株でのロミタピドの機能を確認するために、ロミタピド添加時における細胞の信号伝達効果を調べてみた。
HCT116細胞株でのロミタピドの細胞自己貪食機能の連関性を確認するために、ロミタピド添加時における細胞の信号伝達効果を調べてみた。
ロミタピドの薬物機転と遺伝子との連関性を確認するために、RNA-seq実験を用いて有意に発現する遺伝子及び関連機転に対する分析を、GSEA(Gene set enrichment analysis)及びパスウエイエンリッチメント解析(Pathway enrichment analysis)を用いて行った。
HCT116細胞株においてロミタピドと癌細胞コロニー増殖率との連関性を確認するために、HCT116細胞を培養するウェルにロミタピド添加時の癌細胞コロニー増殖率を調べてみた。
マウス異種移植モデルでのロミタピド抗癌効果を確認するために、腫瘍を移植したマウスにロミタピドを処理した後、腫瘍のサイズ変化を調べてみた。
HCT116、HT29、SW480細胞を96ウェルプレートにシードし、薬物無しで又は単一薬物で処理するか、指定された濃度で組み合わせて48時間処理した。各ウェルにおいてDMSOの最終濃度は<0.01%であったし、処理された量は、細胞から観察可能な毒性がないことを確認した。分析のために次のような薬物を使用した。
同種移植腫瘍マウスモデルにおいて、雌C57B6/Nマウス(野生型、6週齢)に2種のMC38大腸癌とB16F10皮膚黒色腫細胞株を用いて実験を行った。MC38大腸癌とB16F10皮膚黒色腫細胞は、2×105を皮下注射した。2モデルにおいて使用したロミタピドは、45%食塩水、40% PEG300、5% Tween-80、10% DMSOで剤形化した。
下のような方法で2種類の大腸癌患者由来オルガノイド(オルガノイド01-46歳男性、オルガノイド02-74歳女性)を用いて、ロミタピド処理によるオルガノイドの生存力を分析した結果、ロミタピドの容量が増加するほどオルガノイドの生存率が減少することを確認した(図20)。前記結果は、ロミタピドが様々な大腸癌細胞を死滅させることに非常に効果的であることを示す。
大腸癌患者由来オルガノイドを48ウェルプレート(SPL,cat32048)で5~7日培養する。
特定濃度の薬物によるオルガノイドの死滅を観察するために、オルガノイドの死滅によるサイズ変化を算出したし、そのために、オルガノイド初期面積に対する薬物処理後72時間で観察されるオルガノイド面積の割合で算出した(公式1)。
(公式2)特定濃度で薬物の効能(%)=(薬物処理群の損傷したオルガノイド割合/非処理群の損傷したオルガノイド割合)*100
特定濃度での薬物効能=全体効能
結論的に、前記実施例に記載された実験は、ロミタピドがエムトール関連信号伝達を抑制し、細胞の自己貪食機転を活性化させて抗癌効果を起こし、ロミタピドが既存の抗癌剤と併用して投与される場合にシナジー効果を起こすことを示す。
Claims (10)
- エムトール(mTOR)信号伝達抑制剤を有効成分として含む癌の予防又は治療用薬剤学的組成物。
- 前記エムトール信号伝達抑制剤は、ロミタピド、その薬剤学的に許容される塩又はその光学異性質体である、請求項1に記載の癌の予防又は治療用薬剤学的組成物。
- 前記癌は、固形癌である、請求項1に記載の癌の予防又は治療用薬剤学的組成物。
- 前記固形癌は、黒色腫、脳腫瘍、良性星細胞腫、悪性星細胞腫、脳下垂体腺腫、脳髄膜腫、脳リンパ腫、乏枝膠腫、上衣細胞腫、脳幹腫瘍、頭頸部腫瘍、喉頭癌、口腔咽頭癌、鼻腔/副鼻腔癌、鼻咽頭癌、唾液腺癌、下咽頭癌、甲状腺癌、口腔癌、胸部腫瘍、小細胞性肺癌、非小細胞性肺癌、胸腺癌、縦隔腔腫瘍、食道癌、乳癌、男性乳癌、腹部腫瘍、胃癌、肝癌、胆嚢癌、胆道癌、膵癌、小腸癌、大腸癌、直膓癌、肛門癌、膀胱癌、腎臓癌、男性生殖器腫瘍、陰茎癌、前立腺癌、女性生殖器腫瘍、子宮頸癌、子宮内膜癌、卵巣癌、子宮肉腫、膣癌、女性外部生殖器癌、女性尿道癌、骨腫瘍、十二指腸癌、線維肉腫、及び皮膚癌からなる群から選ばれるものである、請求項4に記載の癌の予防又は治療用薬剤学的組成物。
- 前記癌は、血液癌である、請求項1に記載の癌の予防又は治療用薬剤学的組成物。
- 前記血液癌は、急性骨髄球性白血病、急性リンパ球性白血病、慢性骨髄性白血病、慢性リンパ球性白血病、急性単球性白血病、多発性骨髄腫、ホジキンリンパ腫及び非ホジキンリンパ腫からなる群から選ばれるものである、請求項6に記載の癌の予防又は治療用薬剤学的組成物。
- 前記組成物は、抗癌剤をさらに含む、請求項1に記載の癌の予防又は治療用薬剤学的組成物。
- 前記抗癌剤は、フルオロウラシル(Fluorouracil)、イリノテカン(Irinotecan)、抗PD1抗体、ベバシズマブ(Bevacizumab)、カペシタビン(Capecitabine)、セツキシマブ(Cetuximab)、ラムシルマブ(Ramucirumab)、オキサリプラチン(Oxaliplatin)、イピリムマブ(Ipilimumab)、ペンブロリズマブ(Pembrolizumab)、ロイコボリン(Leucovorin)、トリフルリジン/チピラシル(Trifluridine/Tipiracil)、ニボルマブ(Nivolumab)、パニツムマブ(Panitumumab)、レゴラフェニブ(Regorafenib)、アフリベルセプト及びそれらの組合せからなる群から選ばれるものである、請求項8に記載の癌の予防又は治療用薬剤学的組成物。
- 前記抗癌剤は、フルオロウラシル(Fluorouracil)、イリノテカン(Irinotecan)、抗PD1抗体及びそれらの組合せからなる群から選ばれるものである、請求項9に記載の癌の予防又は治療用薬剤学的組成物。
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