JP2023501932A - 単一ペプチドの配列決定及び再構築の方法 - Google Patents

単一ペプチドの配列決定及び再構築の方法 Download PDF

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JP2023501932A
JP2023501932A JP2022525108A JP2022525108A JP2023501932A JP 2023501932 A JP2023501932 A JP 2023501932A JP 2022525108 A JP2022525108 A JP 2022525108A JP 2022525108 A JP2022525108 A JP 2022525108A JP 2023501932 A JP2023501932 A JP 2023501932A
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polypeptide
amino acid
molecules
polypeptides
sample
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JP2023501932A5 (enExample
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ダイアー、マシュー
リード、ブライアン
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Quantum Si Inc
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Quantum Si Inc
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818—Sequencing of polypeptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/205—Aptamer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2570/00—Omics, e.g. proteomics, glycomics or lipidomics; Methods of analysis focusing on the entire complement of classes of biological molecules or subsets thereof, i.e. focusing on proteomes, glycomes or lipidomes

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
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  • Urology & Nephrology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2022525108A 2019-10-28 2020-10-28 単一ペプチドの配列決定及び再構築の方法 Pending JP2023501932A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201962927005P 2019-10-28 2019-10-28
US62/927,005 2019-10-28
US201962940968P 2019-11-27 2019-11-27
US62/940,968 2019-11-27
PCT/US2020/057669 WO2021086918A1 (en) 2019-10-28 2020-10-28 Methods of single-polypeptide sequencing and reconstruction

Publications (2)

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JP2023501932A true JP2023501932A (ja) 2023-01-20
JP2023501932A5 JP2023501932A5 (enExample) 2023-11-08

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US (1) US20210148922A1 (enExample)
EP (1) EP4051806A1 (enExample)
JP (1) JP2023501932A (enExample)
KR (1) KR20220108056A (enExample)
CN (1) CN114929887A (enExample)
AU (1) AU2020376814A1 (enExample)
BR (1) BR112022007937A2 (enExample)
CA (1) CA3159362A1 (enExample)
MX (1) MX2022005094A (enExample)
WO (1) WO2021086918A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025169569A1 (ja) * 2024-02-08 2025-08-14 コニカミノルタ株式会社 核酸アプタマーの配列の決定方法

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201715684D0 (en) * 2017-09-28 2017-11-15 Univ Gent Means and methods for single molecule peptide sequencing
CA3086915A1 (en) 2017-12-29 2019-07-04 Sujal M. Patel Decoding approaches for protein identification
TWI863941B (zh) * 2018-11-15 2024-12-01 美商寬騰矽公司 用於蛋白質定序之方法及組合物
EP4051903A1 (en) 2019-10-29 2022-09-07 Quantum-Si Incorporated Peristaltic pumping of fluids and associated methods, systems, and devices
AU2021276522A1 (en) 2020-05-20 2023-01-05 Quantum-Si Incorporated Methods and compositions for protein sequencing
EP4399528A1 (en) * 2021-09-09 2024-07-17 Nautilus Subsidiary, Inc. Characterization and localization of protein modifications
EP4453579A4 (en) 2021-12-22 2026-04-08 Univ Boston BODIPY-BASED DYES FOR DIGITAL SPACE PROTEOMICS
WO2024086832A1 (en) * 2022-10-21 2024-04-25 Quantum-Si Incorporated Polypeptide cleaving reagents and uses thereof
US20260185996A1 (en) * 2024-08-16 2026-07-02 Quantum-Si Incorporated Methods of controlled cleavage

Citations (1)

* Cited by examiner, † Cited by third party
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WO2020037205A1 (en) * 2018-08-17 2020-02-20 Regeneron Pharmaceuticals, Inc. Methods for de novo protein sequencing

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CA2496294A1 (en) 2005-02-07 2006-08-07 The University Of British Columbia Apparatus and methods for concentrating and separating particles such as molecules
ATE460666T1 (de) * 2008-01-15 2010-03-15 Univ Utrecht Holding Bv Verfahren zur bestimmung der aminosäurensequenz von peptiden
CA2745197A1 (en) * 2008-12-01 2010-06-10 Research Triangle Institute Concurrent identification of multitudes of polypeptides
WO2010065531A1 (en) * 2008-12-01 2010-06-10 Robi David Mitra Single molecule protein screening
WO2016069124A1 (en) * 2014-09-15 2016-05-06 Board Of Regents, The University Of Texas System Improved single molecule peptide sequencing
US9435810B2 (en) * 2013-03-15 2016-09-06 Washington University Molecules and methods for iterative polypeptide analysis and processing
MX2020004559A (es) * 2017-10-31 2020-10-05 Encodia Inc Kits para análisis utilizando codificación y/o etiqueta de ácido nucleico.
WO2019089846A1 (en) * 2017-10-31 2019-05-09 Encodia, Inc. Methods and compositions for polypeptide analysis
AU2019301750A1 (en) * 2018-07-12 2021-01-28 Board Of Regents, The University Of Texas System Molecular neighborhood detection by oligonucleotides

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020037205A1 (en) * 2018-08-17 2020-02-20 Regeneron Pharmaceuticals, Inc. Methods for de novo protein sequencing

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
RAPID COMMUN. MASS SPECTROM., vol. 29, JPN6023013337, 2015, pages 659 - 666, ISSN: 0005587112 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025169569A1 (ja) * 2024-02-08 2025-08-14 コニカミノルタ株式会社 核酸アプタマーの配列の決定方法

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AU2020376814A1 (en) 2022-06-02
MX2022005094A (es) 2022-08-15
WO2021086918A1 (en) 2021-05-06
EP4051806A1 (en) 2022-09-07
CA3159362A1 (en) 2021-05-06
CN114929887A (zh) 2022-08-19
US20210148922A1 (en) 2021-05-20
KR20220108056A (ko) 2022-08-02
BR112022007937A2 (pt) 2022-08-30

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