JP2023052080A - 運動系の疾患の治療のために有用な組成物 - Google Patents
運動系の疾患の治療のために有用な組成物 Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/33—Fibroblasts
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/37—Digestive system
- A61K35/38—Stomach; Intestine; Goblet cells; Oral mucosa; Saliva
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P19/00—Drugs for skeletal disorders
- A61P19/02—Drugs for skeletal disorders for joint disorders, e.g. arthritis, arthrosis
Abstract
Description
本明細書で意図されるように、運動系の疾患、特にウマの疾患は、特に骨関節疾患又は筋骨格系疾患、特に肢の疾患を指す。
好ましくは、本発明に従った個体は動物、特に家畜であり、より好ましくは、ウマ科、特にウマ、ラクダ科、特にラクダ、ヒトコブラクダ又はラマ、イヌ科、特にイヌ、ネコ科、特にネコ、及びダチョウからなる群から選択される。
本明細書において意図されるように、「頬側細胞」という表現は、「口腔の細胞」又は「口腔粘膜の細胞」と同等である。
- 歯肉線維芽細胞を個体から採取する工程;
- 歯肉線維芽細胞を培養する工程;
- 歯肉線維芽細胞又は歯肉線維芽細胞の培養物を個体又は別の個体に投与する工程
を含む。
投与のための歯肉線維芽細胞の調製
約50mgの歯肉の生検をウマ(ウマ0)のメスで行い、次いで酵素的に消化して歯肉線維芽細胞の懸濁液を得る。懸濁液を計数した後、75cm2のフラスコに、完全培地(DMEM 4.5g/lのグルコース+Glutamax(Gibco(登録商標))、ウシ胎児血清(FCS)(Gibco(登録商標))20%、抗生物質/抗真菌剤の存在下)に3x105個の歯肉線維芽細胞を播種し、培地を定期的に交換することにより、5%CO2雰囲気中で90%コンフルエンスまで37℃でインキュベートする。
おそらくは跛行に関連した浅い屈筋腱の腱炎を有する4頭のウマ(ウマ1、2、3、及び4)が、上記に示したように調製された異種歯肉線維芽細胞の注射を受けた。腱の損傷部位は、超音波検査によって確認されている(図を参照)。それは、その周囲と比較してより濃い(暗い)領域として見える。
注射の3ヶ月後、治療部位の超音波観察が行われる(図を参照)。明らかな腱病変は残っていない:治療部位は、超音波検査により均一に見える。動物の運動は正常である。
実施例1に記載したように調製した歯肉線維芽細胞を、跛行と関連した球関節の骨軟骨症を伴うウマ(ウマ5)の球関節に直接注射する。
Claims (12)
- ウマにおける運動系の疾患の予防又は治療における使用のための、頬側細胞を含む組成物。
- ウマにおける腱炎の予防又は治療における、請求項1に記載の使用のための組成物。
- 屈筋腱、特に指骨の浅い若しくは深い屈筋腱の腱炎、又は伸筋腱、特に指骨の外側若しくは指骨の前部の伸筋腱の腱炎の予防又は治療における、請求項2に記載の使用のための組成物。
- ウマにおける関節症の予防又は治療における使用のための、請求項1に記載の使用のための組成物。
- 骨軟骨症又は関節症の予防又は治療における、請求項4に記載の使用のための組成物。
- 馬が跛行を有する、請求項1から5のいずれか1項に記載の使用のための組成物。
- 頬、舌、口蓋、唇粘膜、舌下粘膜、又は歯肉の細胞を含む、請求項1から6のいずれか1項に記載の使用のための組成物。
- 歯肉試料の培養から誘導される、請求項1から7のいずれか1項に記載の使用のための組成物。
- 歯肉線維芽細胞を含む、請求項1から8のいずれか1項に記載の使用のための組成物。
- 500万から4000万個の頬側細胞を含む、請求項1から9のいずれか1項に記載の使用のための組成物。
- 細胞が異種性であり、これはそれらが、組成物が使用されるものとは別のウマから採取されることを意味する、請求項1から10のいずれか1項に記載の使用のための組成物。
- 細胞が自家性であり、これはそれらが、組成物が使用されるものと同じウマから採取されることを意味する、請求項1から10のいずれか1項に記載の使用のための組成物。
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR1660464A FR3058065B1 (fr) | 2016-10-27 | 2016-10-27 | Composition pour le traitement d’une affection de l’appareil locomoteur |
FR1660464 | 2016-10-27 | ||
JP2019544782A JP7203033B2 (ja) | 2016-10-27 | 2017-10-25 | 運動系の疾患の治療のために有用な組成物 |
PCT/EP2017/077336 WO2018077964A1 (fr) | 2016-10-27 | 2017-10-25 | Composition pour le traitement d'une affection de l'appareil locomoteur |
Related Parent Applications (1)
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JP2019544782A Division JP7203033B2 (ja) | 2016-10-27 | 2017-10-25 | 運動系の疾患の治療のために有用な組成物 |
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Publication Number | Publication Date |
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JP2023052080A true JP2023052080A (ja) | 2023-04-11 |
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Application Number | Title | Priority Date | Filing Date |
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JP2019544782A Active JP7203033B2 (ja) | 2016-10-27 | 2017-10-25 | 運動系の疾患の治療のために有用な組成物 |
JP2022208183A Pending JP2023052080A (ja) | 2016-10-27 | 2022-12-26 | 運動系の疾患の治療のために有用な組成物 |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
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JP2019544782A Active JP7203033B2 (ja) | 2016-10-27 | 2017-10-25 | 運動系の疾患の治療のために有用な組成物 |
Country Status (8)
Country | Link |
---|---|
US (1) | US20200268805A1 (ja) |
EP (2) | EP3532080B1 (ja) |
JP (2) | JP7203033B2 (ja) |
CN (1) | CN110234330A (ja) |
AU (2) | AU2017350159B2 (ja) |
CA (1) | CA3041653A1 (ja) |
FR (1) | FR3058065B1 (ja) |
WO (1) | WO2018077964A1 (ja) |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AR047712A1 (es) * | 2002-09-07 | 2006-02-15 | Royal Veterinary College | Metodo de tratamiento de una lesion de tejido esqueletico blando natural administrando una composicion de celulas madre mesenquimatosas |
FR2953723B1 (fr) * | 2009-12-11 | 2012-02-24 | Scarcell Therapeutics | Composition pharmaceutique destinee au traitement des pathologies orthopediques |
US9321993B2 (en) * | 2010-06-17 | 2016-04-26 | The Trustees Of Columbia University In The City Of New York | Tissue culture method for producing cartilage using trimethylamine N-oxide and chondroitinase |
FR2980710B1 (fr) * | 2011-09-30 | 2019-06-21 | Vetbiobank | Composition a base de cellules souches mesenchymateuses viables et apoptotiques et leurs utilisations |
-
2016
- 2016-10-27 FR FR1660464A patent/FR3058065B1/fr active Active
-
2017
- 2017-10-25 EP EP17801349.6A patent/EP3532080B1/fr active Active
- 2017-10-25 WO PCT/EP2017/077336 patent/WO2018077964A1/fr unknown
- 2017-10-25 JP JP2019544782A patent/JP7203033B2/ja active Active
- 2017-10-25 CN CN201780070867.6A patent/CN110234330A/zh active Pending
- 2017-10-25 US US16/345,847 patent/US20200268805A1/en active Pending
- 2017-10-25 EP EP22168015.0A patent/EP4046641A1/fr active Pending
- 2017-10-25 CA CA3041653A patent/CA3041653A1/fr active Pending
- 2017-10-25 AU AU2017350159A patent/AU2017350159B2/en active Active
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2022
- 2022-12-26 JP JP2022208183A patent/JP2023052080A/ja active Pending
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2023
- 2023-10-11 AU AU2023248121A patent/AU2023248121A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
FR3058065A1 (fr) | 2018-05-04 |
AU2023248121A1 (en) | 2023-11-02 |
JP7203033B2 (ja) | 2023-01-12 |
WO2018077964A1 (fr) | 2018-05-03 |
AU2017350159A1 (en) | 2019-06-13 |
US20200268805A1 (en) | 2020-08-27 |
AU2017350159B2 (en) | 2023-11-02 |
EP3532080A1 (fr) | 2019-09-04 |
EP4046641A1 (fr) | 2022-08-24 |
FR3058065B1 (fr) | 2019-02-01 |
EP3532080B1 (fr) | 2022-04-13 |
CN110234330A (zh) | 2019-09-13 |
CA3041653A1 (fr) | 2018-05-03 |
JP2019537625A (ja) | 2019-12-26 |
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