JP2022541273A - 最適化されたタンパク質リンカーおよび使用方法 - Google Patents

最適化されたタンパク質リンカーおよび使用方法 Download PDF

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JP2022541273A
JP2022541273A JP2022503424A JP2022503424A JP2022541273A JP 2022541273 A JP2022541273 A JP 2022541273A JP 2022503424 A JP2022503424 A JP 2022503424A JP 2022503424 A JP2022503424 A JP 2022503424A JP 2022541273 A JP2022541273 A JP 2022541273A
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nucleic acid
domain
terminus
activity
fusion protein
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JP2022541273A5 (https=
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シャーロン リー ガフィー
ジョセフ マシュー ワッツ
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ペアーワイズ プランツ サービシズ， インコーポレイテッド
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Publication of JP2022541273A publication Critical patent/JP2022541273A/ja
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/001—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/21—Endodeoxyribonucleases producing 5'-phosphomonoesters (3.1.21)
    • C12Y301/21001—Deoxyribonuclease I (3.1.21.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
    • C12Y305/04001—Cytosine deaminase (3.5.4.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3513—Protein; Peptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host

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  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2022503424A 2019-07-19 2020-07-17 最適化されたタンパク質リンカーおよび使用方法 Pending JP2022541273A (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2024189563A JP2025020231A (ja) 2019-07-19 2024-10-29 最適化されたタンパク質リンカーおよび使用方法

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US201962876275P 2019-07-19 2019-07-19
US62/876,275 2019-07-19
PCT/US2020/042553 WO2021016086A1 (en) 2019-07-19 2020-07-17 Optimized protein linkers and methods of use

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JP2022541273A true JP2022541273A (ja) 2022-09-22
JP2022541273A5 JP2022541273A5 (https=) 2023-07-18

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US (2) US11072785B2 (https=)
EP (1) EP3987021A4 (https=)
JP (2) JP2022541273A (https=)
CN (2) CN120591235A (https=)
AU (1) AU2020316331B2 (https=)
CA (1) CA3147253A1 (https=)
WO (1) WO2021016086A1 (https=)

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AU2015330699B2 (en) 2014-10-10 2021-12-02 Editas Medicine, Inc. Compositions and methods for promoting homology directed repair
CA3147253A1 (en) * 2019-07-19 2021-01-28 Pairwise Plants Services, Inc. Optimized protein linkers and methods of use
EP4185699A1 (en) 2020-07-21 2023-05-31 Pairwise Plants Services, Inc. Optimized protein linkers and methods of use
US12123006B2 (en) * 2021-05-18 2024-10-22 Shanghaitech University Base editing tool and use thereof
EP4399293A4 (en) * 2021-09-10 2025-10-15 Arbor Biotechnologies Inc COMPOSITIONS COMPRISING A CAS12I POLYPEPTIDE AND USES THEREOF
WO2023164722A1 (en) * 2022-02-28 2023-08-31 Pairwise Plants Services, Inc. Engineered crispr-cas effector proteins and methods of use thereof
CN116217741B (zh) * 2023-02-27 2025-06-27 河北科技大学 一种高效率且低脱靶率的精确基因编辑方法
US12428636B1 (en) 2024-07-08 2025-09-30 Pairwise Plants Services, Inc. Methods and compositions for modification of protospacer adjacent motif specificity of CAS12A

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WO2018176009A1 (en) * 2017-03-23 2018-09-27 President And Fellows Of Harvard College Nucleobase editors comprising nucleic acid programmable dna binding proteins
WO2018213726A1 (en) * 2017-05-18 2018-11-22 The Broad Institute, Inc. Systems, methods, and compositions for targeted nucleic acid editing

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Publication number Publication date
US11708568B2 (en) 2023-07-25
AU2020316331A1 (en) 2022-02-03
CA3147253A1 (en) 2021-01-28
CN114375335B (zh) 2025-07-08
US11072785B2 (en) 2021-07-27
JP2025020231A (ja) 2025-02-12
WO2021016086A1 (en) 2021-01-28
AU2020316331B2 (en) 2026-02-12
CN114375335A (zh) 2022-04-19
EP3987021A4 (en) 2024-05-15
CN120591235A (zh) 2025-09-05
US20210017506A1 (en) 2021-01-21
US20210355466A1 (en) 2021-11-18
EP3987021A1 (en) 2022-04-27

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