JP2022537477A - 機能的エレメントの同定方法 - Google Patents

機能的エレメントの同定方法 Download PDF

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JP2022537477A
JP2022537477A JP2021557446A JP2021557446A JP2022537477A JP 2022537477 A JP2022537477 A JP 2022537477A JP 2021557446 A JP2021557446 A JP 2021557446A JP 2021557446 A JP2021557446 A JP 2021557446A JP 2022537477 A JP2022537477 A JP 2022537477A
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amino acid
score
deletion
deletions
protein
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Japanese (ja)
Inventor
文▲勝▼ 魏
▲軼▼楠 王
悦欣 周
心怡 ▲張▼
▲ディ▼ 岳
▲瑩▼ ▲劉▼
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Peking University
Edigene Inc
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Peking University
Edigene Inc
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1079Screening libraries by altering the phenotype or phenotypic trait of the host
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00Libraries per se, e.g. arrays, mixtures
    • C40B40/04Libraries containing only organic compounds
    • C40B40/06Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B35/00ICT specially adapted for in silico combinatorial libraries of nucleic acids, proteins or peptides
    • G16B35/10Design of libraries
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B35/00ICT specially adapted for in silico combinatorial libraries of nucleic acids, proteins or peptides
    • G16B35/20Screening of libraries
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111General methods applicable to biologically active non-coding nucleic acids
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    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • C12N2310/20Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
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    • C12N2320/00Applications; Uses
    • C12N2320/10Applications; Uses in screening processes
    • C12N2320/11Applications; Uses in screening processes for the determination of target sites, i.e. of active nucleic acids
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    • C12N2330/00Production
    • C12N2330/30Production chemically synthesised
    • C12N2330/31Libraries, arrays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

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  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
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  • Spectroscopy & Molecular Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2021557446A 2019-03-26 2020-03-26 機能的エレメントの同定方法 Pending JP2022537477A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN2019079729 2019-03-26
CNPCT/CN2019/079729 2019-03-26
PCT/CN2020/081283 WO2020192712A1 (en) 2019-03-26 2020-03-26 Method for identifying functional elements

Publications (1)

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JP2022537477A true JP2022537477A (ja) 2022-08-26

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JP2021557446A Pending JP2022537477A (ja) 2019-03-26 2020-03-26 機能的エレメントの同定方法

Country Status (8)

Country Link
US (1) US20220186210A1 (zh)
EP (1) EP3947788A4 (zh)
JP (1) JP2022537477A (zh)
KR (1) KR20220004980A (zh)
CN (1) CN113939617A (zh)
AU (1) AU2020248911B2 (zh)
CA (1) CA3134400A1 (zh)
WO (1) WO2020192712A1 (zh)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3663310A4 (en) 2017-08-04 2021-08-11 Peking University TALE-RVD WITH SPECIFIC DETECTION OF A DNA BASE MODIFIED BY METHYLATION AND APPLICATION OF IT
US11624077B2 (en) 2017-08-08 2023-04-11 Peking University Gene knockout method
CR20220063A (es) 2019-07-12 2022-07-22 Univ Beijing Edición de ácido ribonucleico (arn) dirigido aprovechando la adenosina desaminasa que actúa sobre ácido ribonucleico endógeno (adar) utilizando ácidos ribonucleicos (arn) modificados genéticamente
KR20240003760A (ko) * 2022-06-29 2024-01-09 서울대학교산학협력단 RNA 안정성 또는 mRNA 번역 증가용 신규 조절 엘리먼트,이와 상호작용하는 ZCCHC2, 및 이들의 용도

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016182893A1 (en) * 2015-05-08 2016-11-17 Teh Broad Institute Inc. Functional genomics using crispr-cas systems for saturating mutagenesis of non-coding elements, compositions, methods, libraries and applications thereof
WO2017219027A1 (en) * 2016-06-17 2017-12-21 The Broad Institute Inc. Type vi crispr orthologs and systems
JP2018524018A (ja) * 2015-05-08 2018-08-30 ザ チルドレンズ メディカル センター コーポレイション 胎児型ヘモグロビン再誘導のための、bcl11aエンハンサー機能性領域を標的とする方法
WO2018191388A1 (en) * 2017-04-12 2018-10-18 The Broad Institute, Inc. Novel type vi crispr orthologs and systems

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016182893A1 (en) * 2015-05-08 2016-11-17 Teh Broad Institute Inc. Functional genomics using crispr-cas systems for saturating mutagenesis of non-coding elements, compositions, methods, libraries and applications thereof
JP2018524018A (ja) * 2015-05-08 2018-08-30 ザ チルドレンズ メディカル センター コーポレイション 胎児型ヘモグロビン再誘導のための、bcl11aエンハンサー機能性領域を標的とする方法
WO2017219027A1 (en) * 2016-06-17 2017-12-21 The Broad Institute Inc. Type vi crispr orthologs and systems
WO2018191388A1 (en) * 2017-04-12 2018-10-18 The Broad Institute, Inc. Novel type vi crispr orthologs and systems

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
細胞, vol. 51, no. 3, JPN6023022007, 20 March 2019 (2019-03-20), pages 21 - 24, ISSN: 0005077616 *

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Publication number Publication date
AU2020248911B2 (en) 2022-12-15
US20220186210A1 (en) 2022-06-16
EP3947788A1 (en) 2022-02-09
AU2020248911A1 (en) 2021-11-04
CN113939617A (zh) 2022-01-14
EP3947788A4 (en) 2022-06-08
WO2020192712A1 (en) 2020-10-01
KR20220004980A (ko) 2022-01-12
CA3134400A1 (en) 2020-10-01

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