JP2022531436A - 二次アセテート発酵 - Google Patents
二次アセテート発酵 Download PDFInfo
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- JP2022531436A JP2022531436A JP2021565708A JP2021565708A JP2022531436A JP 2022531436 A JP2022531436 A JP 2022531436A JP 2021565708 A JP2021565708 A JP 2021565708A JP 2021565708 A JP2021565708 A JP 2021565708A JP 2022531436 A JP2022531436 A JP 2022531436A
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- bioreactor
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Abstract
Description
本出願は、2019年7月25日に出願された米国仮特許出願第62/878,528号および2020年3月9日に出願された第62/986,940号の利益を主張する。これらの仮出願の内容は、それらの全体が参照により本明細書に明示的に組み込まれる。
本開示は、ガスをアセテートに変換する一次発酵(第1段階発酵)と、アセテートを標的生成物に変換する二次発酵(第2段階の発酵)との組み合わせに関する。
一次発酵は、ガスをアセテートに変換することができる微生物(「一次微生物」)を使用する。一次発酵は、類似または補完的な成長要件を有する2つのそのような生物の共培養であり得る。したがって、一次発酵は、純粋培養または混合培養を含み得る。
二次発酵は、アセテートを生成物に変換することができる微生物(「二次微生物」)を使用する。二次発酵は、類似または補完的な成長要件を有する2つのそのような生物の共培養であり得る。したがって、一次発酵は、純粋培養または混合培養を含み得る。二次発酵は、嫌気性または好気性プロセスとして実施することができる。
一次発酵および二次発酵は、互いに、および/または上流もしくは下流のプロセスおよびまたは技術と一緒に、何らかの方式で統合され得る。
「発酵」という用語は、基質に化学変化をもたらす代謝プロセスとして解釈されるべきである。例えば、発酵プロセスは、1つ以上の基質を受け取り、1つ以上の微生物を利用することによって1つ以上の生成物を生成する。「発酵」という用語は、1つ以上の基質を受け取り、1つ以上の微生物を利用して1つ以上の生成物を生成するプロセスと解釈されるべきである。好ましくは、発酵プロセスは、1つ以上のバイオリアクターの使用を含む。発酵プロセスは、「バッチ」または「連続」のいずれかとして説明され得る。「バッチ発酵」とは、バイオリアクターが微生物と一緒に原料、すなわち炭素源で満たされ、発酵が完了するまで生成物がバイオリアクター内に留まる発酵プロセスを説明するために使用される。「バッチ」プロセスでは、発酵が完了した後に、生成物を抽出し、次の「バッチ」が始まる前にバイオリアクターを洗浄する。「連続発酵」は、発酵プロセスがより長期間にわたって延長され、発酵中に生成物および/または代謝物が抽出される発酵プロセスを説明するために使用される。好ましくは、発酵プロセスは連続的である。
Claims (28)
- COおよび任意選択でH2から、またはCO2およびH2から少なくとも1つの標的生成物を生成するための方法であって、
i.少なくともCOおよび任意選択でH2、または少なくともCO2およびH2を含むガス状基質を、第1の液体栄養培地中に少なくとも1つの第1の微生物の培養物を含有する第1のバイオリアクターの中に導入し、前記ガス状基質を嫌気的に発酵させて、第1の発酵ブロスにおいて第1の生成物としてアセテートを生成することであって、前記第1の微生物は、Wood-Ljungdahl経路の酵素を発現する、ことと、
ii.前記第1の発酵ブロスの少なくとも一部を、第2の液体栄養培地中の少なくとも1つの第2の微生物の培養物を含有する第2のバイオリアクターに移すことであって、前記第2の微生物は、前記第1の微生物とは異なる種である、こと、および前記第1の生成物を発酵させて、第2の発酵ブロスにおいて少なくとも第1の標的生成物を生成することを行うことと、を含む、方法。 - 前記第1の液体栄養培地が、前記第2の微生物によって利用されるが、前記第1の微生物によって利用されないある量の栄養素を含む、請求項1に記載の方法。
- 前記第1の液体栄養培地が、前記第2の微生物によって利用されるが、前記第1の微生物によって利用されない栄養素を含む、請求項1に記載の方法。
- 前記第2の液体栄養培地が、前記第1の発酵ブロスである、請求項1に記載の方法。
- 前記第2の発酵ブロスが、前記第1のバイオリアクターに再び移される、請求項1に記載の方法。
- 前記第1の標的生成物が、単一細胞タンパク質、3-ヒドロキシプロピオネート、ポリヒドロキシアルカノエート、C5~C20アルコール、有機酸、ケトン、および脂質から選択される、請求項1に記載の方法。
- 前記第1の発酵ブロスが、2,3-ブタンジオールをさらに含む、請求項1に記載の方法。
- 前記第1の発酵ブロスの少なくとも一部を、前記第2のバイオリアクターに移す前に、アセテートまたは第2の生成物が、前記第1の発酵ブロスから取り出される、請求項1に記載の方法。
- 前記第2のバイオリアクターにおける発酵後、前記第1のバイオリアクターで生成された第2の生成物が、前記第2の発酵ブロスから分離される、請求項1に記載の方法。
- 前記第1のバイオリアクター、前記第2のバイオリアクター、または前記第1のバイオリアクターおよび前記第2のバイオリアクターにおける前記培養物が、共培養物を含む、請求項1に記載の方法。
- 前記第1の標的生成物が、脂質ではない、請求項1に記載の方法。
- 前記第1の標的生成物が、エタノール、アセトン、プロパノール、ブタノール、2,3-ブタンジオール、アセテート、ブチレート、プロピオネート、カプロエート、プロピレン、ブタジエン、イソブチレン、イソブチルアセテート、ポリヒドロキシアルカノエート、スクシネート、メバロネート、イタコン酸、モネリン、およびエチレンから選択される、請求項1に記載の方法。
- i.前記第2のバイオリアクターから少なくともCO2およびO2を含む排ガスを得る工程と、
ii.前記排ガスから前記O2の少なくとも一部を取り出して、O2枯渇排ガスを形成する工程と、
iii.前記O2枯渇排ガスの少なくとも一部を、前記第1のバイオリアクターに再循環させる工程と、をさらに含む、請求項1に記載の方法。 - 前記排ガスからの前記O2の少なくとも一部の前記取り出しが、圧力スイング吸着、膜分離、塩基性溶液によるスクラビング、溶媒を使用する吸収、またはこれらの任意の組み合わせのうちの1つ以上の段階を使用して達成される、請求項13に記載の方法。
- 前記排ガスからの前記O2の少なくとも一部の前記取り出しに由来する前記O2を、前記第2のバイオリアクターに再循環させることをさらに含む、請求項13に記載の方法。
- 前記第1のバイオリアクターにおける前記少なくとも1つの第1の微生物が、Acetobacterium、Moorella、Clostridium、Pyrococcus、Eubacterium、Desulfobacterium、Carboxydothermus Acetogenium、Acetoanaerobium、Butyribacterium、Peptostreptococcus、Ruminococcus、Oxobacter、およびMethanosarcinaから選択される、請求項1に記載の方法。
- 前記第1のバイオリアクターにおける少なくとも1つの第1の微生物が、Acetobacterium woodiiである、請求項1に記載の方法。
- 前記第1のバイオリアクターにおける少なくとも1つの第1の微生物が、Clostridium autoethanogenum、Clostridium ljungdahlii、およびまたはClostridium ragsdaleiから選択される、請求項1に記載の方法。
- 栄養素が、前記第1の液体栄養培地に添加されて、前記第1の発酵ブロスにおける第2の生成物の生成を増加させ、前記第2の生成物が、前記第2のバイオリアクターへと、前記第1の発酵ブロスの前記一部として移され、前記第2のバイオリアクターにおいてバイオマス生成を刺激するか、または前記第2のバイオリアクターにおいて発酵されて、前記第1の標的生成物または第2の標的生成物を形成する、請求項1に記載の方法。
- COおよび任意選択でH2、またはCO2およびH2を含むガス流を、前記第1のバイオリアクターから回収し、前記ガス流を前記第1のバイオリアクターに再循環させることをさらに含む、請求項1に記載の方法。
- 前記第2の発酵ブロスの少なくとも一部を、前記第1のバイオリアクターに再循環させることをさらに含む、請求項1に記載の方法。
- 前記第1の発酵ブロスの少なくとも一部を、前記第2のバイオリアクターに通す前に、前記第1の発酵ブロスから前記第1の微生物を取り出し、前記第1の微生物を、前記第1のバイオリアクターに再循環させることをさらに含む、請求項1に記載の方法。
- 前記第2の発酵ブロスから前記第2の微生物を取り除き、前記第2の微生物を前記第2のバイオリアクターに再循環させることをさらに含む、請求項1に記載の方法。
- 電解槽を使用して、前記ガス状基質においてH2の少なくとも一部および/または前記第2のバイオリアクターの中に導入されるO2の少なくとも一部を発生させることをさらに含む、請求項1に記載の方法。
- 前記第2の微生物が、酵母である、請求項1に記載の方法。
- 前記第2の微生物が、Escherichia coliである、請求項1に記載の方法。
- 前記第2のバイオリアクターにおける培養物が、共培養物を含む、請求項1に記載の方法。
- 前記第2の発酵が、好気性である、請求項1に記載の方法。
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PCT/US2020/043417 WO2021016523A1 (en) | 2019-07-25 | 2020-07-24 | Secondary acetate fermentation |
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US20210277430A1 (en) * | 2020-03-09 | 2021-09-09 | Lanzatech, Inc. | Fermentation process for the production of lipids |
US20220325216A1 (en) * | 2021-04-09 | 2022-10-13 | Lanzatech, Inc. | Intermittent feedstock to gas fermentation |
WO2023137333A1 (en) * | 2022-01-11 | 2023-07-20 | Synata Bio, Inc. | Methods for efficient fermention broth recycle |
WO2023215589A1 (en) * | 2022-05-06 | 2023-11-09 | Lanzatech, Inc. | Integration of renewable chemical production into oil, gas, petroleum, and chemical processing and infrastructure |
WO2023250389A1 (en) * | 2022-06-21 | 2023-12-28 | Lanzatech, Inc. | Microorganisms and methods for the continuous co-production of high-value, specialized proteins and chemical products from c1-substrates |
CN115197002B (zh) * | 2022-06-24 | 2024-04-09 | 昊特(湖北)流体技术有限公司 | 一种具有内循环效果的微生物肥料发酵罐 |
WO2024076509A1 (en) * | 2022-10-04 | 2024-04-11 | Jupeng Bio (Hk) Limited | Extraction of nutrient supplement product using enzyme digestion of cell mass |
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US20160122787A1 (en) * | 2014-10-31 | 2016-05-05 | Lanzatech New Zealand Limited | Fermentation process for the production of lipids |
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NZ546496A (en) * | 2006-04-07 | 2008-09-26 | Lanzatech New Zealand Ltd | Gas treatment process |
US20100159539A1 (en) * | 2008-03-13 | 2010-06-24 | Ascon Miguel | Methods and systems for producing biofuels and bioenergy products from xenobiotic compounds |
US20110177564A1 (en) * | 2010-01-15 | 2011-07-21 | Massachusetts Institute Of Technology | Bioprocess and microbe engineering for total carbon utilization in biofuel production |
WO2013036147A2 (en) * | 2011-09-08 | 2013-03-14 | Lanzatech New Zealand Limited | A fermentation process |
MY187786A (en) | 2016-07-27 | 2021-10-22 | Evonik Operations Gmbh | N-acetyl homoserine |
WO2018039319A1 (en) * | 2016-08-23 | 2018-03-01 | Papoutsakis Eleftherios T | Syntrophic co-cultures and uses thereof |
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CN112955561A (zh) | 2021-06-11 |
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WO2021016523A1 (en) | 2021-01-28 |
US11788103B2 (en) | 2023-10-17 |
AU2020316096A1 (en) | 2021-12-02 |
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