JP2022167716A - Antibacterial and antiviral agent - Google Patents

Abstract

To provide an antibacterial and antiviral agent that has antibacterial and antiviral properties and can suppress the proliferation of bacteria and virus, the agent being safe and applicable to various articles.SOLUTION: An antibacterial and antiviral agent according to the present invention contains a plurality of platinum nanoparticles with a nano particle size as an active ingredient. Alternatively, an antibacterial and antiviral agent according to the present invention contains a platinum nanoparticle solution containing platinum nanoparticles with an average particle size of 1-10 nm.SELECTED DRAWING: None

Description

TECHNICAL FIELD The present invention relates to an antibacterial and antiviral agent containing a plurality of nanosized platinum nanoparticles as active ingredients.

Conventionally, antibacterial and antiviral finishing has been applied to various products to prevent the growth of bacteria that cause unpleasant odors, or to prevent colds and viral diseases. Various antibacterial agents and antiviral agents have also been proposed.

Currently, the novel coronavirus infectious disease (COVID-19) is spreading, and in order to prevent infection from the new coronavirus (SARS-CoV-2), or to spread the new coronavirus (SARS-CoV-2) to others In order to prevent infection, antibacterial and antiviral properties are required for products used in all aspects of daily life, and various products with antibacterial and antiviral properties are on the market.

On the other hand, in order to impart antibacterial and antiviral properties to products that have been used for a long time, floors and walls of houses, etc., it is possible to easily impart antibacterial and antiviral effects, and to use safe and inexpensive antibacterial agents. There is also a need for antiviral agents.

JP 2008-056592 A

Patent Document 1 discloses an antibacterial treatment liquid characterized by comprising a colloidal solution containing platinum particles with an average particle size of 1 to 10 nm. Patent Document 1 describes the antibacterial properties of platinum nanoparticles, but does not describe the antiviral properties, and naturally describes the antiviral properties against the new coronavirus (SARS-CoV-2). It has not been. In addition, in the current situation where the novel coronavirus infectious disease (COVID-19) is prevalent, there is a demand for an antiviral agent that can impart an antiviral effect by a simple method for the safety and security of consumers.

Accordingly, an object of the present invention is to provide a safe and inexpensive antibacterial and antiviral agent that also has antibacterial and antiviral properties, particularly antiviral properties against the novel coronavirus (SARS-CoV-2). Another object of the present invention is to provide an antibacterial and antiviral agent that can easily impart antibacterial and antiviral properties to a wide variety of articles.

The antibacterial and antiviral agent according to the present invention preferably contains a plurality of nano-sized platinum nanoparticles as active ingredients.

The antibacterial and antiviral agent according to the present invention preferably comprises a platinum nanoparticle solution containing a plurality of nanosized platinum nanoparticles.

Antibacterial and antiviral agents according to the present invention should have antiviral properties against the novel coronavirus (SARS-CoV-2).

The antibacterial and antiviral agent according to the present invention preferably has an antiviral activity value of 3.0 or more.

The antibacterial and antiviral agent according to the present invention may be a spray product obtained by filling a spray container with the antibacterial and antiviral agent.

In the antibacterial and antiviral agent of the present invention, by including a plurality of platinum nanoparticles, the "antibacterial" and "antiviral" properties of the platinum nanoparticles provide both antibacterial and antiviral properties. agent can be provided.

In the antibacterial and antiviral agent according to the present invention, a plurality of platinum nanoparticles can suppress the growth of bacteria and viruses. In particular, it can be effectively used as an antiviral agent to prevent the novel coronavirus infection (COVID-19) because it has a growth-inhibiting effect against the new coronavirus (SARS-CoV-2). can.

In addition, the antibacterial and antiviral agent according to the present invention is a spray product filled in a spray container, so that the antibacterial and antiviral effects can be easily obtained by spraying it on the article to which the antibacterial and antiviral effects are desired. can.

The antibacterial and antiviral agent according to the present invention is characterized by containing a plurality of nano-sized platinum nanoparticles as active ingredients.

The platinum nanoparticles, which are the active ingredients of the antibacterial and antiviral agents according to the present invention, may be nanosized platinum particles, preferably platinum nanoparticles with an average particle diameter of 1 to 10 nm. good.

Also, the antibacterial and antiviral agent according to the present invention is preferably a platinum nanoparticle solution containing a plurality of nanosized platinum nanoparticles. The solvent of the solution is preferably water such as purified water. This is because it can be safely used for a wide variety of articles.

The platinum nanoparticle solution contains nanosized platinum particles in the solution. The particle size of the platinum particles contained in the solution may be nano-sized, preferably platinum nanoparticles having an average particle size of 1 to 10 nm.

The platinum nanoparticle solution that can be used for the antibacterial and antiviral agent according to the present invention is characterized by being prepared by reducing platinum ions, and contains, for example, platinum nanoparticles with an average particle size of 1 to 10 nm. One consisting of a colloidal solution can be mentioned. Such a colloidal solution can be prepared, for example, by reacting chloroplatinate ions (PtCl4 ²⁻ ) with a reducing agent capable of reducing platinum ions such as ascorbic acid in water to reduce the platinum ions. can be done. The concentration of platinum ions (chloroplatinate ions) in water for preparing the colloidal solution is preferably 0.001 to 0.1 mol/L, and the concentration of the reducing agent is 5 to 20 times the concentration of platinum ions. It is desirable to In order to stably exist platinum ions in water, it is desirable to add a hydroxycarboxylic acid such as citric acid (which may be in the form of a salt such as sodium salt) as a stabilizer. The concentration of the stabilizer in water is desirably 0.5 to 2 times the concentration of platinum ions.

The antibacterial and antiviral agent according to the present invention can be used as a safe and secure antibacterial and antiviral agent because all raw materials (purified water, ascorbic acid, citric acid, platinum) are food additives.

The antibacterial and antiviral agent according to the present invention may be a spray product filled with the antibacterial and antiviral agent in a spray container. By filling a spray container with the antibacterial and antiviral agent according to the present invention to prepare a spray product, it is possible to easily impart antibacterial and antiviral properties to a wide variety of products. The spray container consists of a spray body containing antibacterial and antiviral agents and a spray head having an atomizing mechanism attached to the spray body. The spray mechanism is not particularly limited, and may be, for example, a push-type spray type or a trigger-type spray type.

The antibacterial and antiviral agents according to the present invention can be used in textile products such as masks, clothes, towels, curtains, etc., kitchen utensils, bathing utensils, bathtubs, washstands, toilets, hand disinfection, and the like. For example, a spray product filled with the antibacterial and antiviral agent containing the above platinum nanoparticle solution in a spray container may be sprayed on the floor of a house and allowed to dry naturally. By spraying a spray product filled with an antibacterial and antiviral agent consisting of a platinum nanoparticle solution in a spray container, the platinum nanoparticles can be attached to the floor material, giving antibacterial and antiviral effects to the floor of the house. can do.

In addition, since the antibacterial and antiviral agent according to the present invention suppresses the growth of bacteria due to its antibacterial effect, it is possible to obtain a deodorant effect by suppressing the growth of bacteria that cause unpleasant odors. It can be suitably used as an antibacterial and deodorant for sanitary products and floors such as toilets.

In addition, the present invention may be textile products, resin moldings, ceramic moldings, etc. coated with the antibacterial and antiviral agents described above. Textile products include, for example, sanitary products such as masks, clothes, towels, and curtains. Examples of resin molded products include toothbrush handles and bristles, trays for transporting food, and bathtubs. Examples of ceramic moldings include toilets and washbasins.

Furthermore, the present invention may be textile products, resin moldings, ceramic moldings, etc. containing the antibacterial and antiviral agents described above. Textile products include, for example, sanitary products such as masks, clothes, towels, and curtains. Examples of resin molded products include toothbrush handles and bristles, trays for transporting food, and bathtubs. Examples of ceramic moldings include toilets and washbasins.

[Preparation of platinum nanoparticle solution]
0.208 g of potassium chloroplatinate (K ₂ PtCl ₄ ) was dissolved in 50 mL of pure water to prepare a 0.01 mol/L (0.050 g/L as Pt(0)) potassium chloroplatinate aqueous solution. Separately, 0.129 g of sodium citrate was dissolved in 50 mL of pure water to prepare a 0.01 mol/L (0.066 g/mL) sodium citrate aqueous solution, and 0.881 g of ascorbic acid was dissolved in 50 mL of pure water. to prepare a 0.1 mol/L (0.452 g/L) ascorbic acid aqueous solution. After adding 50 mL of the above 0.01 mol/L potassium chloroplatinate aqueous solution to 1800 mL of pure water, 50 mL of 0.01 mol/L sodium citrate aqueous solution and 50 mL of 0.1 mol/L ascorbic acid aqueous solution were added, respectively, and about 5 By vigorously stirring for 1 minute, a solution containing platinum colloidal particles having an average particle diameter of 4 nm (hereinafter referred to as “platinum nanoparticles”), ie, a platinum nanoparticle solution, was obtained. The average particle size was measured by a field emission scanning electron microscope in which the solution was dispersed and dried. It should be noted that such "platinum nanoparticle solutions" are sold and available on the trading market.

[Antibacterial test 1]
An antibacterial activity test against MRSA (methicillin-resistant Staphylococcus aureus) was performed on an aqueous platinum nanoparticle solution obtained by diluting the "platinum nanoparticle solution" prepared in Example 1 with 400 times water.

The method of the antibacterial test is based on JIS1902:2008 quantitative test (bacterial liquid absorption method), and the method of measuring the number of viable bacteria is based on the pour plate culture method. In the antibacterial test, 0.4 g of platinum nanoparticle solution was impregnated into cotton cloth, and then 0.2 ml of MRSA bacterial solution was inoculated. Table 1 shows the results of the antibacterial test against MRSA (methicillin-resistant Staphylococcus aureus).

Here, the "growth value" is the logarithmic value of the growth of viable bacteria within 18 hours after inoculation on a standard cotton cloth containing no platinum nanoparticles.

"Bactericidal activity value" is an index showing how much the inoculated bacteria are reduced after a certain period of time, and is expressed in logarithm. That is, the evaluation of the "bactericidal activity value" is
1) If the "Bactericidal activity value" is negative ... "The number of given bacteria is increasing"
2) When the "Bactericidal activity value" is 0 ... "The number of bacteria given is zero"
3) If the "Bactericidal activity value" is positive ... "The number of given bacteria is decreasing."
4) When the "Bactericidal activity value" is 1 ... "The number of given bacteria has decreased to one tenth"
5) When the "Bactericidal activity value" is 3 ... "The number of given bacteria is reduced to 1/1000"
becomes.

In addition, the "bacteriostatic activity value" is a numerical value indicating how much the treated sample inhibited the growth of bacteria compared to the sample without antibacterial treatment, and is a numerical value for judging the antibacterial and deodorant effect. is. According to the JIS standard, the evaluation criteria for "having antibacterial and deodorizing effects" is when the "bacteriostatic activity value" is 2.0 or more.

Table 1 shows that the platinum nanoparticle solution has sufficient antibacterial properties against MRSA (methicillin-resistant Staphylococcus aureus).

[Antibacterial test 2]
An antibacterial activity test against Staphylococcus aureus was performed on an aqueous platinum nanoparticle solution obtained by diluting the "platinum nanoparticle solution" prepared in Example 1 with 400 times water.

The method of the antibacterial test similarly applies JIS 1902:2008 quantitative test (bacterial liquid absorption method), and the method of measuring the number of viable bacteria is based on the pour plate culture method. In the antibacterial test, 0.4 g of platinum nanoparticle solution was impregnated into cotton cloth, and then 0.2 ml of Staphylococcus aureus bacterial solution was inoculated. Table 2 shows the results of the antibacterial test against Staphylococcus aureus.

Table 2 shows that the platinum nanoparticle solution has sufficient antibacterial properties against Staphylococcus aureus.

[Antiviral test]
An antiviral test against the novel coronavirus (SARS-CoV-2) was performed on an aqueous solution of platinum nanoparticles obtained by diluting the "platinum nanoparticle solution" prepared in Example 1 with 50 times water.

[Test conditions]
The test conditions were as follows.
・Test sample: Platinum nanoparticle solution 50-fold dilution ・Test virus: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
giving)
- Control sample (Negative control): Phosphate buffered saline (PBS)

[Test method]
Host cells were infected with virus, EMEM was added, cultured at 37°C for a predetermined time, centrifuged at 1,000 xg at 4°C for 15 minutes, and the supernatant was diluted 10-fold with EMEM to prepare a test virus suspension. liquid.

[Host cell verification test]
Prior to the main test, host cell verification tests (cytotoxicity confirmation test and virus cell sensitivity confirmation test) were conducted. The host cell verification test shall be judged based on the following criteria.
・Cytotoxicity: None ・Confirmation of cell susceptibility to virus: lg (viral infectious titer of PBS (PFU/mL)) - lg (viral infectious titer of sample (PUF/mL)) ≤ 0.5

[Cytotoxicity confirmation test]
(1) Add 0.1 mL of EMEM to 1.9 mL of test sample and stir thoroughly. This is used as the test solution.
(2) Add 0.1 ml of the test solution to 0.9 ml of the drug inactivating agent and stir thoroughly.
(3) Prepare a 10-fold dilution series using DMEM containing 2% FBS.
(4) Confirm the presence or absence of cytotoxicity in each dilution series by plaque assay.

[Virus cell susceptibility confirmation test]
(1) Add 0.1 mL of EMEM to 1.9 mL of test sample and stir thoroughly. This is used as the test solution.
(2) Add 0.5 ml of the test solution to 4.5 ml of a drug deactivator (a solution obtained by diluting SCDLP 10-fold with DMEM containing 2% FBS) and stir thoroughly.
(3) Prepare a 10-fold dilution series using DMEM containing 2% FBS.
(4) Add 1/100 of each dilution series in (3) to a virus suspension adjusted to 4 to 6×10 ⁴ PFU/mL using EMEM.
(5) Allow to stand at room temperature for 10 minutes.
(6) Measure the virus infectivity titer per 1 mL of each dilution series by the plaque assay method to confirm the susceptibility of the cells to the virus.

宿主細胞検証試験の結果を表３に示す。試験液を薬剤不活化剤で１０倍希釈することにより、検体の影響を受けずにウイルス感染価測定ができることを確認した。なお、ウイルス細胞の感受性確認試験における試験ウイルス懸濁液濃度は、４．７×１０^４ＰＦＵ／ｍｌであった。

The results of host cell validation studies are shown in Table 3. It was confirmed that the virus infectivity titer can be measured without being affected by the sample by diluting the test solution 10-fold with the drug inactivating agent. The test virus suspension concentration in the virus cell sensitivity confirmation test was 4.7×10 ⁴ PFU/ml.

[Main test]
This test method was performed as follows.
(1) Add 0.1 mL of test virus suspension (suspension concentration: 1.2×10 ⁷ PFU/ml) to 1.9 mL of test sample and stir thoroughly.
(2) Allow to stand at 25° C. for 18 hours. This is used as the test solution.
(3) Inactivate the test solution under conditions where inactivation was confirmed in the host cell verification test. This is used as a reaction stopping solution.
(4) Assuming that the reaction stop solution in (3) above is 100, prepare a 10-fold dilution series with DMEM containing ² % FBS, and measure the virus infectivity titer per 0.1 ml of the reaction stop solution by a plaque assay method, Calculate the virus infection titer (antiviral activity value) per 1 ml of the test solution. If the antiviral activity value is 3.0 or more, it can be judged that there is a sufficient antiviral effect against the virus. Table 4 shows the results of antiviral tests against the novel coronavirus (SARS-CoV-2).

From Table 4, the negative control showed almost no change in the common logarithm of the virus infectivity even after 18 hours of action, whereas the platinum nanoparticle solution showed a decrease in the common logarithm of the virus infectivity. The common logarithmic difference (antiviral activity value) from negative control after 18 hours was 3.3, and the platinum nanoparticle solution reduced 99.9% of the new coronavirus (SARS-CoV-2). It has been shown that a 50-fold diluted aqueous solution of nanoparticles has a high antiviral effect.

[Deodorant test]
The deodorant property of an aqueous platinum nanoparticle solution prepared by diluting the "platinum nanoparticle solution" prepared in Example 1 with 800 times water was tested. The test method is based on the certification standards for deodorant processed textile products of the Textile Evaluation Technology Council. The test conditions were as follows: initial gas concentrations were 1000 ppm (10 ml) for ammonia, 50 ppm (10 ml) for acetic acid, and about 38 ppm (10 ml) for isovaleric acid, and the measurement time was 2 hours after the start of the test. A deodorizing effect reduction rate of 75% or more is acceptable.

Table 5 shows that it has high deodorizing performance against ammonia, acetic acid, and isovaleric acid, which are sources of offensive odors.

In addition, the present invention can be implemented with various improvements, modifications, and changes based on the knowledge of those skilled in the art without departing from the spirit of the present invention.

Since the antibacterial and antiviral agent according to the present invention contains a plurality of nano-sized platinum nanoparticles as active ingredients, it can be used as a safe antibacterial and antiviral agent for a wide variety of articles.

Claims (5)

An antibacterial and antiviral agent containing, as an active ingredient, a plurality of nanosized platinum nanoparticles. An antibacterial and antiviral agent comprising a platinum nanoparticle solution containing a plurality of nanosized platinum nanoparticles. 3. The antibacterial and antiviral agent according to claim 1 or 2, which has antiviral properties against novel coronavirus (SARS-CoV-2). 4. The antibacterial and antiviral agent according to claim 3, which has an antiviral activity value of 3.0 or more. A spray product comprising a spray container filled with the antibacterial and antiviral agent according to any one of claims 1 to 4.