JP2022016360A - Antibacterial/antiviral composition and antibacterial/antiviral fiber product - Google Patents

Abstract

To provide an antibacterial/antiviral composition that has high safety for the human body and exhibits antiviral as well antibacterial properties, and an antibacterial/antiviral fiber product.SOLUTION: An antibacterial/antiviral composition contains a polycation compound and water, and the content of the polycation compound is 0.06-6 mass% of the whole composition.SELECTED DRAWING: None

Description

The present invention relates to a highly safe antibacterial / antiviral agent composition, and an antibacterial / antiviral textile product obtained by adhering or impregnating the textile product with the antibacterial / antiviral agent composition.

The life and economic loss of humans and livestock caused by viruses such as human influenza virus, bird influenza virus (similar to human influenza virus), corona virus, norovirus, Ebola hemorrhagic fever virus, AIDS virus, and bacteria such as salmonella and pathogenic Escherichia coli. It is enormous. In recent years, norovirus causes mass food poisoning in winter, and deaths due to complications of the elderly and immunocompromised persons have been reported. In addition, the danger of pandemics caused by coronavirus and avian influenza virus has been pointed out by WHO. In recent years, food poisoning caused by Salmonella and pathogenic Escherichia coli has come to occur regardless of the season.

In response to such threats, there are increasing demands for removing bacteria and viruses adhering to doorknobs, handrails, tables, etc. to prevent the spread of infection. For example, for coronavirus, it is recommended to wipe off with a disinfectant solution diluted with water and a high concentration alcohol and a surfactant such as sodium hypochlorite and sodium linear alkylbenzene sulfonate. There is.
Further, for bacteria, as an alcohol-free antibacterial agent composition, for example, an antibacterial agent composition containing ε-polylysine and / or a salt thereof, an electrolyte having a pH buffering ability, and an amino acid is disclosed. It is said that antibacterial properties can be imparted by wiping with a cage (Patent Document 1) using this antibacterial agent composition.

Japanese Unexamined Patent Publication No. 2004-67586

However, when a high concentration of alcohol is used as an antibacterial / antiviral agent composition, it may cause rough skin and may cause discomfort due to its high volatility. In addition, there is a problem that alcohol volatilizes instantly and its effect as an antibacterial / antiviral agent composition cannot be maintained.
In addition, sodium hypochlorite has a problem that it is difficult to handle for daily use because it may have an adverse effect on the human body depending on its concentration. On the other hand, when a surfactant such as sodium linear alkylbenzene sulfonate is used, the safety is high, but the efficacy is generally low, and there is a problem that the efficacy cannot be exhibited in a short time unless the concentration is set high. ..
Further, the antibacterial agent composition of Patent Document 1 is highly safe for the human body and can be expected to have an antibacterial effect, but has a problem that the effect on an antiviral agent is unknown.

The present invention has been made in view of such circumstances, and is an antibacterial / antiviral agent composition, antibacterial / antiviral property, which is highly safe for the human body and is excellent not only in antibacterial property but also in antiviral property. The purpose is to provide a textile product having a virus.

［３］ 上記一般式（１）で示されるポリカチオン化合物が、ポリ－オキシエチレン（ジメチルイミノ）エチレン（ジメチルイミノ）エチレンジクロライドである［２］記載の抗菌・抗ウイルス剤組成物。
［４］ さらに炭素数１～６のアルコールを含有し、上記アルコールの含有量が、組成物全体の４０質量％以下である［１］～［３］のいずれかに記載の抗菌・抗ウイルス剤組成物。
［５］ 上記アルコールが、エタノールである［４］記載の抗菌・抗ウイルス剤組成物。
［６］ 上記ポリカチオン化合物の含有量が、組成物全体の０．１８～６質量％である、［４］または［５］記載の抗菌・抗ウイルス剤組成物。
［７］ アニオン界面活性剤を含有しない［１］～［６］のいずれかに記載の抗菌・抗ウイルス剤組成物。
［８］ さらに塩化ベンザルコニウムを含有する［１］～［７］のいずれかに記載の抗菌・抗ウイルス剤組成物。
［９］ エンベロープ型ウイルスに対して用いられる［１］～［８］のいずれかに記載の抗菌・抗ウイルス剤組成物。
［１０］ 上記エンベロープ型ウイルスが、インフルエンザウイルスおよびコロナウイルスの少なくとも一方である［９］記載の抗菌・抗ウイルス剤組成物。
［１１］ 噴霧剤として用いられる、［１］～［１０］のいずれかに記載の抗菌・抗ウイルス剤組成物。
［１２］ ［１］～［１１］のいずれかに記載の抗菌・抗ウイルス剤組成物が、繊維製品に付着または含浸されてなる抗菌・抗ウイルス性を有する繊維製品。 In order to achieve the above object, the present invention provides the following [1] to [12].
[1] An antibacterial / antiviral agent composition containing a polycation compound and water, wherein the content of the polycation compound is 0.06 to 6% by mass of the entire composition.
[2] The antibacterial / antiviral agent composition according to [1], wherein the polycation compound is represented by the following general formula (1).

[3] The antibacterial / antiviral agent composition according to [2], wherein the polycation compound represented by the above general formula (1) is poly-oxyethylene (dimethylimino) ethylene (dimethylimino) ethylene dichloride.
[4] The antibacterial / antiviral agent according to any one of [1] to [3], which further contains an alcohol having 1 to 6 carbon atoms and the content of the alcohol is 40% by mass or less of the whole composition. Composition.
[5] The antibacterial / antiviral agent composition according to [4], wherein the alcohol is ethanol.
[6] The antibacterial / antiviral agent composition according to [4] or [5], wherein the content of the polycation compound is 0.18 to 6% by mass based on the total composition.
[7] The antibacterial / antiviral agent composition according to any one of [1] to [6], which does not contain an anionic surfactant.
[8] The antibacterial / antiviral agent composition according to any one of [1] to [7], which further contains benzalkonium chloride.
[9] The antibacterial / antiviral agent composition according to any one of [1] to [8] used for enveloped viruses.
[10] The antibacterial / antiviral agent composition according to [9], wherein the enveloped virus is at least one of influenza virus and coronavirus.
[11] The antibacterial / antiviral agent composition according to any one of [1] to [10], which is used as a spraying agent.
[12] A textile product having antibacterial / antiviral properties, wherein the antibacterial / antiviral agent composition according to any one of [1] to [11] is adhered to or impregnated into a textile product.

In the present invention, the "textile product" is mainly composed of cotton, linen, wool, silk and other natural fibers, chemical fibers such as rayon and sufu, synthetic fibers such as nylon, polyester, vinylon and acrylic, and these. The purpose is to include any of so-called textile products such as woven fabrics, non-woven fabrics, threads, and aggregates.

That is, the present inventors have made extensive studies to develop an antibacterial / antiviral agent composition that is highly safe for the human body and is excellent not only in antibacterial properties but also in antiviral properties. As a result, they have found that a compound containing the above polycation compound in water at a specific ratio exhibits not only antibacterial properties but also antiviral properties and is highly safe for the human body, and has reached the present invention.

As described above, according to the antibacterial / antiviral agent composition of the present invention, it is not necessary to contain a substantially high concentration of alcohol and a surfactant such as sodium hypochlorite and sodium linear alkylbenzene sulfonate. Therefore, it can be handled safely on a daily basis. Moreover, the active ingredient does not volatilize instantly, and high antibacterial and antiviral effects can be maintained even at a low concentration.

Among them, when the polycation compound is represented by the above general formula (1), higher antibacterial and antiviral effects can be exhibited.

Further, when the polycation compound represented by the above general formula (1) is poly-oxyethylene (dimethylimino) ethylene (dimethylimino) ethylene dichloride, further higher antibacterial and antiviral effects can be exhibited.

Further, when an alcohol having 1 to 6 carbon atoms is further contained and the content of the alcohol is 40% by mass or less of the whole composition, a particularly excellent antibacterial and antiviral effect can be exhibited by the combined use of these alcohols. can.

Furthermore, when the alcohol is ethanol, it not only exhibits higher antibacterial and antiviral effects, but also has an excellent usability.

Further, when the content of the polycation compound is 0.18 to 6% by mass of the whole composition and the content of the alcohol having 1 to 6 carbon atoms is 40% by mass or less of the whole composition. It has a higher antibacterial and antiviral effect, and has an excellent usability.

If it does not contain an anionic surfactant, it can exhibit even higher antibacterial and antiviral effects.

Further, when benzalkonium chloride is further contained, a more remarkable antiviral effect can be exhibited.

When it is used against an enveloped virus, it can exert a higher antiviral effect.

Further, when the enveloped virus is at least one of influenza virus and coronavirus, a higher antiviral effect can be exhibited.

When used as a spray agent, even if the target area is wide, a sufficient amount can be easily spread over the entire area, and sufficient antibacterial and antiviral effects are applied to a wider object. Can be demonstrated.

According to the antibacterial / antiviral textile product in which the antibacterial / antiviral agent composition of the present invention is adhered to or impregnated into the textile product, it is safe and sufficient even if it is handled on a daily basis. It has antibacterial and antiviral properties. Moreover, it does not require a high concentration of alcohol, and not only has an immediate effect, but also can exhibit excellent antibacterial and antiviral effects over a long period of time.

Next, a mode for carrying out the present invention will be specifically described, but the present invention is not limited thereto.

<Antibacterial / antiviral agent composition>
The antibacterial / antiviral agent composition of the present invention (hereinafter, may be referred to as “composition”) has a polycation compound and water, and the content of the polycation compound is set in a specific range. It is a thing. These will be described in detail below.

[Polycation compound]
A polycationic compound is used in the antibacterial / antiviral agent composition of the present invention, and is preferably a polycationic compound represented by the following general formula (1).

In the above general formula (1), the alkyl groups having 1 to 6 carbon atoms represented by R ² , R ³ , R ⁵ , and R ⁶ include a methyl group, an ethyl group, a propyl group, a butyl group, a pentyl group, and a hexyl group. Any of the linear type, the isopropyl group, the isobutyl group, and the branched chain type of the isopentyl group may be used. Of these, a linear alkyl group is preferable. Further, an alkyl group having 4 or less carbon atoms is preferably used from the viewpoint of enhancing ease of handling, antibacterial property and antiviral property. Note that n in the general formula (1) is a positive integer and is not particularly limited, but is preferably in the range of 4 to 55. Further, in consideration of antibacterial / antiviral properties, handleability, etc., it is preferable that the weight average molecular weight of the polycation compound is 1000 to 10000.

Examples of the polycation compound used in the present invention include a quaternary salt of an alkyl (C = 1 to 5 or H) amine / epichlorohydrin adduct, a polyethylene polyamine / dimethylamine / epichlorohydrin polycondensate, and a poly-2-hydroxypropyldimethyl. Examples thereof include ammonium chloride, and among them, the polycation compound represented by the above general formula (1) is preferably used because of its excellent antibacterial and antiviral properties, and in particular, the repetition represented by the following formula (2). Poly-oxyethylene (dimethylimino) ethylene (dimethylimino) ethylene dichloride having a unit (hereinafter, may be referred to as “PMIEC”) is preferable.

In the present invention, as the polycation compound, a compound produced according to a known method can be used, but a commercially available product may be used. In addition, the polycation compound can be used alone or in combination of two or more.

The polycation compound in the antibacterial / antiviral agent composition of the present invention has the above polycation compound content when the polycation compound is diluted with water or the like to prepare an antibacterial / antiviral agent composition. It is 0.06 to 6% by mass of the whole composition, preferably 0.3 to 3% by mass, and more preferably 0.48 to 1.8% by mass.

[water]
The water used in the present invention is not particularly limited, but for example, tap water, soft water, pure water (ion-exchanged water, distilled water, RO water (water passed through a back-penetration membrane), purified water) and the like can be used. Of these, soft water, ion-exchanged water, RO water, and distilled water are preferably used. These may be used alone or in combination of two or more.

The antibacterial / antiviral agent composition of the present invention usually consists of a uniform aqueous solution, and the balance other than each component is water. For example, the content of the water is 50 to 50 in the whole composition. It is preferably 99.94% by mass, more preferably 60 to 98% by mass, and even more preferably 65 to 90% by mass.

In the antibacterial / antiviral agent composition of the present invention, the content of the above polycation compound and optionally used alcohol having 1 to 6 carbon atoms is set high, and water is kept within the above range at the time of use. It may be diluted with and used.

[Alcohol with 1 to 6 carbon atoms]
The antibacterial / antiviral agent composition of the present invention preferably contains an aliphatic alcohol having 1 to 6 carbon atoms. In particular, it is preferable to use ethanol because it enhances antibacterial and antiviral properties and has a good feeling on the skin. Usually, the alcohol can exert an excellent effect in correlation with the polycation compound when it is 5% by mass or more, 10% by mass or more, particularly 15% by mass or more of the whole composition. On the other hand, if the amount of alcohol used is large, it causes rough skin, but in the present invention, an excellent effect can be exhibited even with a relatively low content due to the interaction with the polycation compound. For example, 40% by mass or less, preferably 30% by mass or less, and more preferably 25% by mass or less of the whole composition is sufficient. These may be used alone or in combination of two or more.

When the alcohol having 1 to 6 carbon atoms is contained, the compounding ratio of the polycation compound (A) to the alcohol (B) having 1 to 6 carbon atoms is usually (A): (B) in terms of mass ratio. = It is preferably in the range of 1: 5 to 1: 500, and more preferably in the range of 1:25 to 1:85. That is, when the polycation compound and the alcohol are used in combination, the synergistic effect of the polycation compound and the alcohol is obtained even if the alcohol concentration is as low as less than 50% by mass, which is said to exert antibacterial and antiviral effects. As a result, denaturation of the membranes / proteins constituting the fungus / virus can be caused, and the fungus / virus can be effectively killed.
When the alcohol having 1 to 6 carbon atoms is contained, the content of the polycation compound is preferably 0.18 to 3% by mass, preferably 0.30 to 1.5% by mass. More preferred. That is, when the polycation compound and the alcohol are used in combination, a synergistic effect is observed with each other, and both the polycation compound and the alcohol can sufficiently exhibit antibacterial and antiviral properties with a smaller content. .. For example, in order to exhibit antibacterial and antiviral properties with alcohol alone, as described above, an alcohol content of 50% by mass or more is usually required.

In particular, PMIEC is used as the polycation compound, and the content thereof is 0.6 to 1.8% by mass of the whole composition, and ethanol is contained as the alcohol, and the content thereof is 10 to 30% by mass. When it is%, high antibacterial / antiviral properties tend to increase.
The higher the content of PMIEC, the higher the antibacterial / antiviral effect. However, from the viewpoint of safety, the content is preferably 6% by mass or less, more preferably 4.8. It is less than mass%.
Further, from the viewpoint that the wiping residue of the composition is less likely to be left, it is preferable that the content of PGIEC is small, and in particular, PGIEC 0.3 to 0.6% by mass and ethanol 10 to 30% by mass are used in combination. It is preferable to do so.

In addition to these, the antibacterial / antiviral agent composition of the present invention may contain, for example, a deodorant, a preservative, or a fragrance in order to improve the antibacterial / antiviral property and maintain the effect, if necessary. , Oily ingredients, thickeners, moisturizers, pigments, emulsifiers, pH adjusters, ceramides, sterols, antioxidants, single oxygen scavengers, UV absorbers, whitening agents, anti-inflammatory agents, surfactants, Known arbitrary components such as other antibacterial agents and other antiviral agents can be blended. These can be used alone or in combination of two or more.

Specific examples of the optional component include benzalkonium chloride, benzethonium chloride, dialkyldimethylammonium chloride and the like, and in particular, a cationic surfactant can be used because the performance of the polycation compound is not impaired. It is preferable to use it. Among them, it is preferable to use benzalkonium chloride because the performance of each other can be enhanced by combining with the above polycation compound. When benzalkonium chloride is used as an optional component, the preferable content is preferably 0.005 to 0.5% by mass, more preferably 0.01 to 0.2% by mass, based on the entire composition. preferable. If the content of benzalkonium chloride is too high, the irritation when it adheres to the skin tends to increase. When benzalkonium chloride is used as an optional component, the compounding ratio of the polycation compound (A) and benzalkonium chloride is usually a mass ratio of the polycation compound (A): benzalkonium chloride = 600: 1. It is preferably in the range of ~ 2: 1 and more preferably in the range of 300: 1 to 5: 1. When the compounding ratio of the polycation compound (A) and benzalkonium chloride is within the above range, the synergistic effect is more excellent, and the immediate effect and the durability tend to be excellent.

However, in the present invention, it is preferable that the anionic surfactant is not contained as a substantial component among the known optional components. The inclusion of the above-mentioned anionic surfactant may cause a problem of aggregation with the polycation compound and accompanying deterioration of efficacy, which is not preferable.
Examples of the anionic surfactant include fatty acids, alkylamide sulfates, alkylarylsulfonic acids, oxyethylated alkyl ether carboxylic acids, oxyethylated alkyl ether sulfates, oxyethylated alkylaryl ether sulfates, sulfosuccinic acids, and α. -Sodium salts such as olefin sulfonic acid, calcium salts, monoethanolamine salts, diethanolamine salts, triethanolamine salts and (n- or iso-) propylamine salts, phosphate ester salts and the like can be mentioned.
In addition, the above-mentioned "substantially not contained" includes not only the case where it is not contained at all but also the case where a small amount such that the effect cannot be expected in a short time (within 5 minutes) is blended. Its content means less than 0.1% by weight with respect to the total composition.

The method for producing the antibacterial / antiviral agent composition of the present invention is not particularly limited, and for example, water constituting most of the composition is first charged, and then each component is added to the above water. It can be obtained by stirring until uniform.

According to the above configuration, since it is not necessary to contain a high concentration of alcohol, it is possible to reduce the possibility of adversely affecting the human body, and since it has low volatility, it is possible to bring it into contact with the object for a sufficient time. It is possible to continuously exert antibacterial and antiviral properties. Further, since it is not necessary to contain a surfactant such as sodium hypochlorite or sodium linear alkylbenzene sulfonate, it is easy to handle and can be used on a daily basis. Further, since it has both antibacterial and antiviral properties and is used as an aqueous uniform aqueous solution, it can be used for spraying or dipping on an object to be treated, and is excellent in convenience.

The antibacterial / antiviral agent composition of the present invention exerts its effect when it comes into contact with an object. For example, when it is used as a spraying agent, the above composition can be brought into contact with a wide range at one time. Suitable.

Further, by using the antibacterial / antiviral agent composition of the present invention in household products, industrial materials, medical products, hygiene products, cooking products made of various resin materials such as resin sheets and resin films, antibacterial agents are used. -An antibacterial effect can be imparted. Examples of the method of use include spraying or impregnating the above-mentioned composition with each of the above-mentioned products, materials and the like. Further, the surface of each of the above-mentioned products and materials may be wiped with a cloth or the like impregnated with the above-mentioned composition.
Examples of the above household products and industrial materials include, for example, nursing seats, shower curtains, car seats, seat covers, interior materials such as ceiling materials, tents, insect / bird nets, partition sheets, air conditioning filters, and cleaning. Machine filters, masks, tablecloths, underlays, apron, wallpaper, wrapping paper, etc. Examples of the medical supplies include medical beds, wheelchairs, sterilization bags and the like. Examples of the hygienic products include toilet bowls, cleaning brushes, dust boxes, disposable gloves, disposable masks, tissue paper, wet tissues and the like. Examples of the cooking utensils include a serving table and a tray.

<Antibacterial and antiviral textile products>
Further, by adhering or impregnating the textile product with the antibacterial / antiviral agent composition of the present invention, a textile product having an antibacterial / antiviral effect can be obtained. As the above-mentioned usage method, for example, the above-mentioned composition may be sprayed on the above-mentioned textile product and adhered to the surface thereof. Further, the textile product may be impregnated with the composition.

The textile product may be the fiber itself or a product using the fiber, and as described above, the textile product prepared before the processing may be in the form of a final product as it is. The textile product may be deformed or combined with other members to change the shape or composition to make a final product.

Examples of such products include various industrial materials and household products, and the types of fibers used in the textile products are, as described above, cotton, linen, wool, silk and other natural fibers, polyester resins, and the like. Examples thereof include synthetic fibers such as polyamide-based resin, acrylic resin, polyurethane-based resin and polyolefin-based resin, semi-synthetic fibers such as cellulose-based resin and acetate resin, and composites and mixtures thereof. In addition to these, synthetic fibers mixed with components other than synthetic fibers (metals, inorganic substances, etc.) and synthetic fibers and natural fibers such as cotton, acetate, rayon, wool, and silk are blended products. Can be done.

In the present invention, examples of the form of the target textile product include threads, strings, ropes, fabrics (woven fabric, knitted fabric, non-woven fabric) and the like. Specific examples include disposable masks and wet tissues.

<Performance of antibacterial / antiviral agent composition and its evaluation>
The antibacterial / antiviral agent composition of the present invention has antibacterial / antiviral properties, and exhibits excellent antiviral properties particularly against enveloped viruses. In addition, since the antibacterial and antiviral properties last for a long time, the object to which the above composition comes into contact can be used cleanly for a long period of time.

In the antibacterial / antiviral agent composition of the present invention, the types of bacteria exhibiting an antibacterial effect are described in detail, and examples thereof include gram-negative bacteria, gram-positive bacteria and fungi. Examples of the gram-negative bacterium include, but are not limited to, Escherichia coli, Salmonella, Vibrio parahaemolyticus, Klebsiella pneumoniae, and Pseudomonas aeruginosa. Examples of the gram-positive bacterium include, but are not limited to, methicillin-resistant Staphylococcus aureus (MRSA), Legionella, Bacillus cereus that forms spores, and Bacillus subtilis. Further, examples of the fungus include, but are not limited to, Aspergillus niger, Penicillium niger, Aspergillus niger, and red yeast (Rhodotorula).

In the antibacterial / antiviral agent composition of the present invention, the types of viruses having an antiviral effect will be described in detail. Pox virus and orthomixovirus (typically, human influenza virus and bird influenza virus) which are viruses having an envelope. ), Coronavirus, paramixovirus, arenavirus, rabdovirus, retrovirus, bunyavirus, herpesvirus, togavirus, papovavirus, porbovirus, phyllovirus, etc., among others, human influenza virus, bird influenza virus and coronavirus. On the other hand, a high effect is recognized, and it is extremely effective against the new corona virus (SARS-CoV-2). In addition, adenovirus, poliovirus, norovirus, rotavirus and the like, which are viruses having no envelope, can also be mentioned.

Next, in the present invention, a test method for evaluating antiviral property and antibacterial property will be described.

<Test method for antiviral evaluation>
[Fiber application]
0.5 mL of the composition of the present invention was uniformly sprayed onto the entire surface of a non-woven fabric having a length of 16 cm, a width of 15 cm and a thickness of 0.05 cm. Then, the above-mentioned non-woven fabric was air-dried and subjected to the test.
Then, in Example 1 and Comparative Examples 1 to 3, influenza virus (envelope type, including human influenza virus) is used as a target virus, and the plaque measurement method shown below (General Incorporated Association Textile Evaluation Technology Council, anti-virus processing). The anti-virus activity value was calculated according to the proposal of the preparatory committee). According to JIS L 1922 Annex G, the antiviral property is evaluated as "effective" when the antiviral activity value is 2.0 or more and less than 3.0, and "sufficient" when the antiviral activity value is 3.0 or more. It is evaluated as "effect".
On the other hand, for Examples 5 and 6, the new coronavirus (envelope type) was used as the target virus, and the antiviral activity value was calculated based on the antiviral measurement method described later. The above-mentioned antiviral activity value of 5 means that the infectious titer of the virus (the number of virus particles having cell infectivity) was reduced to 1/10000 (the inactivation of the virus was realized). There is.

-Plaque measurement method Measure according to JIS L 1922 Annex G. The target virus is influenza virus (envelope type, including human influenza virus), and canine kidney-derived cells are used. Then, after contacting the target material with the virus solution at 25 ° C. for 2 hours, the virus solution and the canine kidney-derived cells are post-cultured, and the increase / decrease (infection titer) of the virus is calculated in the cultured cells, and the blank (untreated material) is used. Calculate the logarithmic difference to determine the antiviral activity value.

-Antiviral measurement method The target virus is the new coronavirus (envelope type). As the new coronavirus, SARS-CoV-2 (JPN / TY / WK521) is used. Then, after contacting the target material with the virus solution at room temperature for 10 minutes, the virus solution is post-cultured with VeroE6 / TMPRSS2 cells, the increase / decrease (infection value) of the virus is calculated in the cultured cells, and a blank (untreated) is obtained. Calculate the logarithmic difference from the material) to obtain the antiviral activity value.

[Composition (liquid)]
The test virus solution was prepared by diluting a virus solution prepared to be about ¹⁰⁸ PFU / mL or more in MCM medium 10-fold with sterilized purified water.
0.5 mL of the above test virus solution was placed in a tube containing 0.5 mL of the composition of the present invention to prepare a test solution, and the test solution was stirred for 10 seconds and then left at room temperature (about 25 ° C.).
After 5 minutes and 30 minutes of standing, the above test solution was stirred for 10 seconds, 0.1 mL of each was collected, placed in 9.9 mL of SCDLP medium (inactivated solution), stirred, and diluted 10-fold in steps. went.
MDCK cells cultured on a separately prepared 6-well plate were inoculated with 0.1 mL of the diluted solution of each of the above steps and adsorbed at 34 ° C. for 1 hour. Then, the diluted solution of each step was washed away, an agar medium was layered, and the cells were cultured for 3 days.
After culturing, the number of plaques formed was counted, and the infectious titer of the liquid in which the test body and the test virus were brought into contact was calculated. The comparative control was sterilized purified water.
It has been confirmed in advance that the antiviral property of the composition of the present invention can be inactivated by diluting it 100-fold with SCDLP medium.
-Viruses used: Influenza virus (Influenza A virus (H3N2) ATCC VR-1679) was used for Examples 1 and Comparative Examples 1 to 3, and SARS-CoV-2 (JPN / TY) was used for Examples 5 and 6. / WK521) was used.
-MDCK cells (host cells): ATCC CCL-34 was used for Examples 1 and Comparative Examples 1 to 3, and VeroE6 / TMPRSS2 cells were used for Examples 5 and 6.

<Test method for antibacterial evaluation>
[Composition (liquid)]
The following bacteria were inoculated into the SCD liquid medium, and the bacterial solution diluted to a final concentration of ¹ × 105 CFU / mL was used as the test bacterial solution.
In each well of 48 microplates prepared separately, 890 μL of SCD liquid medium, 100 μL of the above test bacterial solution, and 10 μL of diluted solution stepwise diluted with sterile physiological saline so that the concentration of the composition of the present invention becomes 10 to 10000 ppm. It was put in and statically cultured at 37 ° C. for 24 hours. After culturing, the growth of bacteria in each well was visually confirmed as liquid turbidity, and the minimum inhibitory concentration (MIC) was determined.
-Bacteria used: Staphylococcus aureus

Next, examples of the present invention will be described together with comparative examples. However, the present invention is not limited to the following examples.

[Examples 1 to 6, Comparative Examples 1 to 3]
First, the antibacterial / antiviral agent composition of the present invention was prepared with the formulations shown in Table 1. The following polycation compounds, deodorants, and preservatives were used.

-Polycation compound: Poly-oxyethylene (dimethylimino) Ethylene (dimethylimino) Ethylene dichloride-Deodorant: Pancil BA-200E-1 (manufactured by Release Kagaku Kogyo Co., Ltd.)
-Preservative: Benzoisothiazolinone (BIT)

Then, with respect to the compositions of Examples 1 to 6 and Comparative Examples 1 to 3, the antiviral property was evaluated according to the following criteria by determining the virus infectivity as described above. Further, the antibacterial property was evaluated according to the following criteria by obtaining the MIC as described above. It should be noted that the antiviral properties of Examples 2 to 4 have not been evaluated, and the antiviral properties of Examples 5 and 6 have been evaluated based on stricter criteria than those of Examples 1 to 4. ..
These results are also shown in Table 1 below.

[Evaluation of antiviral property (fiber application)]
<Example 1, Comparative Examples 1 to 3>
The antiviral activity value calculated above was evaluated based on the following indexes.
+++ (Sufficient effect): Antiviral activity value is 3.0 or more.
++ (Passed effect): Antiviral activity value is 2.0 or more and less than 3.0.
+ (Slight effect): Antiviral activity value is 1.0 or more and less than 2.0.
-(No effect): Antiviral activity value is less than 1.0.
<Examples 5 and 6>
The antiviral activity value calculated above was evaluated based on the following indexes.
◎ (Sufficient effect): Antiviral activity value is 5.0 or more.
〇 (Passed effect): Antiviral activity value is 3.0 or more and less than 5.0.
△ (with some effect): Antiviral activity value is 2.0 or more and less than 3.0.
× (No effect): Antiviral activity value is less than 2.0.

[Evaluation of antiviral property (composition (liquid))]
<Example 1, Comparative Examples 1 to 3>
The common logarithm of the virus infectivity titer calculated above was obtained and evaluated based on the following index.
+++ (Sufficient effect): The common logarithm reduction value of the viral infectious value is 3 or more.
++ (Passed effect): The common logarithm reduction value of the viral infectious value is 2 or more and less than 3.
+ (Slightly effective): The common logarithm reduction value of the viral infectious value is 1 or more and less than 2.
-(No effect): The common logarithm reduction value of the viral load is less than 1.
<Examples 5 and 6>
The common logarithm of the virus infectivity titer calculated above was obtained and evaluated based on the following index.
◎ (Sufficient effect): The common logarithm reduction value of the viral infectious value is 5 or more.
〇 (Passed effect): The common logarithm reduction value of the viral infectious value is 3 or more and less than 5.
△ (with some effect): The common logarithm reduction value of the viral infectious value is 2 or more and less than 3.
× (No effect): The common logarithm reduction value of the viral load is less than 2.

[Evaluation of antibacterial properties]
<Examples 1 to 6>
The MIC obtained above was evaluated based on the following indexes.
+++ (with sufficient antibacterial effect): MIC is less than 100 ppm.
++ (passed antibacterial effect): MIC is 100 ppm or more and less than 2000 ppm.
+ (Slight antibacterial effect): MIC is 2000 ppm or more and less than 10000 ppm.
-(No antibacterial effect): MIC is 10,000 ppm or more.

From the above results, it was found that all of Examples 1 to 6 containing a specific amount of the polycation compound can exhibit antibacterial properties. Among them, it was found that Example 1 containing the polycation compound and ethanol in the present invention can exhibit higher antibacterial and antiviral properties. Further, regarding the antiviral property, it was found that Examples 5 and 6 cleared the stricter criteria than Example 1 and the effect was significantly improved as compared with Example 1.
On the other hand, in Comparative Examples 1 to 3 containing no polycation compound, a sufficient effect on antiviral property could not be obtained, and even if the composition (liquid) was effective at 50% by mass or more, the fiber was applied. It turned out to be ineffective.

INDUSTRIAL APPLICABILITY The present invention can be used as a highly safe antibacterial / antiviral agent composition.

Claims (12)

An antibacterial / antiviral agent composition containing a polycation compound and water, wherein the content of the polycation compound is 0.06 to 6% by mass of the entire composition. The antibacterial / antiviral agent composition according to claim 1, wherein the polycation compound is represented by the following general formula (1).

The antibacterial / antiviral agent composition according to claim 2, wherein the polycation compound represented by the general formula (1) is poly-oxyethylene (dimethylimino) ethylene (dimethylimino) ethylene dichloride. The antibacterial / antiviral agent composition according to any one of claims 1 to 3, further comprising an alcohol having 1 to 6 carbon atoms and having an alcohol content of 40% by mass or less based on the total composition. The antibacterial / antiviral agent composition according to claim 4, wherein the alcohol is ethanol. The antibacterial / antiviral agent composition according to claim 4 or 5, wherein the content of the polycation compound is 0.18 to 6% by mass based on the whole composition. The antibacterial / antiviral agent composition according to any one of claims 1 to 6, which does not contain an anionic surfactant. The antibacterial / antiviral agent composition according to any one of claims 1 to 7, further comprising benzalkonium chloride. The antibacterial / antiviral agent composition according to any one of claims 1 to 8, which is used for an enveloped virus. The antibacterial / antiviral agent composition according to claim 9, wherein the enveloped virus is at least one of influenza virus and coronavirus. The antibacterial / antiviral agent composition according to any one of claims 1 to 10, which is used as a spraying agent. A textile product having antibacterial / antiviral properties, wherein the antibacterial / antiviral agent composition according to any one of claims 1 to 11 is adhered to or impregnated into a textile product.