JP2022103067A - Deodorant method of odor derived from microbe - Google Patents

Abstract

To provide a deodorant method of odor derived from microbes which deodorizes by decomposing or inactivating an odorous compound that microbes produce or an enzyme relating to the production of the odorous compound.SOLUTION: A deodorant method of odor derived from microbes has the following step 1 and step 2: <Step 1> by contacting a treating solution containing the following (a) component with microbes to take the (a) component into a microbe body, under the presence of iron (III) ion present in a protein structure constituting the microbes in a microbe body, a reaction of generating hydrogen peroxide by the (a) component and dissolved oxygen in the microbe to generate hydrogen peroxide, and a reaction generating iron (II) ion by the (a) component and the iron (III) ion, where (a) component is one kind or more compounds selected from ascorbic acid, and ascorbic acid derivatives; <Step 2> in the microbe body, the hydrogen peroxide obtained in the step 1 and iron (II) ion derived from the microbe are reacted to generate a hydroxy radical to decompose or inactivate an odorous compound that the microbe produce or an enzyme relating to the production of the odorous compound.SELECTED DRAWING: None

Description

The present invention relates to a method for deodorizing odors derived from microorganisms.

Examples of unpleasant odors generated when textile products are used in daily life include body odor, urine odor, and fecal odor. For example, in a nursing care site, textile products after washing do not smell, but urine that adheres after wearing causes urine odor, which causes discomfort to the wearer and people around him in daily life, which is a problem. .. This is because the odorless urine odor precursor (glucuronide conjugate) contained in the urine attached to the textile product is metabolized by the microorganism (β-glucuronidase active bacterium) attached to the textile product by the enzyme (β-glucuronidase). This is because urine odor substances (phenolic compounds such as p-cresol) are produced by decomposition. In this way, the odorous compound produced by the microorganisms attached to the textile product, which is considered to be the cause of the malodor generated when the textile product is worn and used, or the enzyme involved in the production of the odorous compound is decomposed or inactivated to cause an unpleasant odor. Technology to reduce the problem is required.

Non-Patent Document 1 discloses the correlation between the structure of ascorbic acid and the bactericidal activity.
Non-Patent Document 2 discloses the antibacterial action of ascorbic acid fatty acid ester.

Akira Murata et al., Vitamin Vol. 83, No. 2 (February issue) 2009, p.49-52 Written by Shoko Kato et al., Sanitation Magazine, Vol.29, No.5, p.331-335

In daily life, unpleasant odors may be generated due to urine or sweat from people, or pet odors may be generated due to pets who are becoming more involved in life with people. The cause of this is that the precursors of odorous compounds from humans and pets are converted into odorous compounds by the metabolism of microorganisms. Many means have been considered for sterilization and eradication of microorganisms, and although the method of bringing peroxides and hydroxyl radicals into direct contact with microorganisms is expected to be effective, the effects on the human body and the environment cannot be ignored.

The present invention is an enzyme that produces hydrogen peroxide and hydroxyl radical in a microorganism by applying a compound that has little effect on the human body and the environment, and is involved in the production of an odorous compound or an odorous compound produced by the microorganism. Provided is a method for deodorizing odors derived from microorganisms, which deodorizes by decomposing or inactivating.

The present invention relates to a method for deodorizing odors derived from microorganisms, which comprises the following steps 1 and 2.
<Process 1>
The treatment liquid containing the following component (a) is brought into contact with the microorganism, and the component (a) is taken into the body of the microorganism in the presence of iron (III) ions present in the protein structure constituting the microorganism in the body of the microorganism. , (A) A step of producing hydrogen peroxide by a component and dissolved oxygen in a microorganism, and a reaction of (a) producing iron (II) ion by a component and iron (III) ion.
(A) Ingredient: One or more compounds selected from ascorbic acid and ascorbic acid derivatives
<Process 2>
In the body of a microorganism, the hydrogen peroxide obtained in step 1 is reacted with iron (II) ions derived from the microorganism to generate hydroxyl radicals, which are involved in the production of odorous compounds or odorous compounds produced by the microorganisms. The step of degrading or inactivating an enzyme.

According to the present invention, by applying a compound having little influence on the human body or the environment into the body of a microorganism, the odorous compound produced by the microorganism or the enzyme involved in the production of the odorous compound is deodorized by decomposing or inactivating it. , Deodorizing methods of odors derived from microorganisms are provided. By using the odor deodorizing method derived from the microorganism of the present invention, for example, the enzyme (β-glucuronidase) metabolized by the microorganism (β-glucuronidase active bacterium) adhering to the textile product is decomposed, and the urine odor substance (p-) is decomposed. It can suppress the generation of phenolic compounds such as cresol) and suppress urine odor.

The deodorizing method of the present invention produces hydrogen peroxide and hydroxyl radicals in the microorganism, and decomposes or inactivates the odorous compound produced by the microorganism or the enzyme involved in the production of the odorous compound to deodorize the action mechanism. Is not necessarily clarified in detail, but it can be considered as follows. The present invention is not limited to this.
The deodorizing method of the present invention is to bring the component (a) into the microorganism by contacting the treatment liquid containing the component (a) of the present invention, which has little influence on the human body, the deodorized object and the environment, with the microorganism. Start. It is considered that there are relatively hydrophobic parts such as protein structures having a higher structure and hydrophilic parts such as body fluids having a large amount of water in the microbial body. The component (a) taken into the microbial body has a hydrophilic / hydrophobic property peculiar to its structure, and is distributed in a specific ratio between the hydrophobic part and the hydrophilic part in the microbial body. exist. Some protein structures in microorganisms are complexed with iron (III) ions or stabilized in the state of iron (III) hydroxide or the like. In step 1 of the present invention, at the hydrophobic site formed by the protein structure, the component (a) distributed to the hydrophobic site in the presence of the iron (III) ion and the dissolved oxygen in the microbial body are iron (III). ) Ions act as catalysts and cause a reduction reaction of oxygen to generate hydrogen peroxide. Further, in this hydrophobic site, iron (III) ions present in the protein structure constituting the microorganism in parallel react with the component (a) to generate iron (II) ions. Some of the reduced iron (II) ions and hydrogen peroxide move to hydrophilic sites because they do not interact with protein structures in particular. Next, in step 2, the hydrogen peroxide generated in step 1 and the iron (II) ion cause a Fenton reaction at a hydrophilic site to generate hydroxyl radical. In this reaction, iron (II) ions are oxidized to iron (III) ions, and these iron (III) ions cause a reduction reaction with the component (a) distributed in hydrophilic sites to cause iron (II). ) Becomes an ion. Then, since the reduced iron (II) ion can react with hydrogen peroxide further generated in step 1, the hydroxyl radical generation reaction in step 2 can occur continuously. The generated hydroxyl radicals exert a deodorizing effect by decomposing or inactivating the odorous compound produced by the microorganism or the enzyme involved in the production of the odorous compound. As described above, in the deodorizing method of the present invention, the two steps occur in different environments in the microorganism body, and even if the starting point is from the production of hydrogen peroxide in step 1, they occur almost simultaneously, and the hydrophobic site is affected. It is important that the iron (III) ion is present, and that the component (a) is incorporated and present in both the hydrophobic and hydrophilic sites in order for this reaction to occur continuously. Is. Therefore, especially when the component (a) has a specific S (Oc) / S ( _H2O ) ratio, it is taken up into the microbial body and then distributed to the ratio in the hydrophobic site and the hydrophilic site. It is considered preferable because it creates an environment in which steps 1 and 2 occur quickly.

<Process 1>
In step 1, the treatment liquid containing the following component (a) is brought into contact with the microorganism, the component (a) is taken up into the microorganism, and iron (III) present in the protein structure constituting the microorganism in the microorganism. ) In the presence of ions, it is a step of performing a reaction of generating hydrogen peroxide by the component (a) and dissolved oxygen in a microorganism, and a reaction of producing iron (II) ion by the component (a) and iron (III) ion. ..
(A) Ingredient: One or more compounds selected from ascorbic acid and ascorbic acid derivatives

In the present invention, a microorganism refers to a minute organism whose individual existence cannot be discerned by the human eye, for example, a prokaryote such as a bacterium or a nematode, a eukaryote such as yeast or mold, or a lower algae. Includes viruses and the like.
Further, as the microorganism to be brought into contact with the treatment liquid of the present invention, iron (III) such as a protein having a higher-order structure such as complex formation of iron (III) ions or stabilization as iron hydroxide (III) or the like. Any microorganism may be used as long as it contains a protein in which ions are present as a constituent protein. , Corinebacterium spp., Regionella spp., Lactobacillus spp., Clostridium spp., Lenza coccus, Hemophilus spp. One or more species selected from the fungi can be mentioned, and from the viewpoint of the odor-producing ability of the fungus, it is preferable to use staphylococci such as Escherichia coli and yellow cocci, Micrococcus spp., Moraxella spp., Asinetobacta spp. One or more species selected from fungi and Corinebacterium spp., More preferably one or more species selected from staphylococci such as Escherichia coli and yellow cocci.
Further, in the deodorizing method of the present invention, it is preferable to perform step 1 and step 2 on the microorganisms present in the textile product.

By contacting the treatment liquid of the present invention with a microorganism, the component (a) is taken up into the microorganism, and the component (a) and the iron (III) ion present in the protein structure constituting the microorganism are present in the microorganism. Below, the component (a) is reacted with the dissolved oxygen in the microorganism to generate hydrogen peroxide. Further, in parallel with the reaction, iron (III) ions are reduced by the component (a), and iron (II) ions derived from the microorganism are generated in the microorganism.
Specific examples of the protein forming the structure in which iron (III) ions are present include one or more selected from ferritin, methemoglobin, transferrin, and lactoferrin, and iron (iron for hydrogen peroxide production reaction). III) From the viewpoint of ion supply, one or more selected from ferritin and methemoglobin is preferable, and ferritin is more preferable.

In the present invention, "contacting the treatment liquid of the present invention with microorganisms" means the act of directly contacting the microorganisms adhering to the deodorant object (for example, immersing the treatment liquid with the microorganisms adhering to the treatment liquid). (Acts such as spraying and coating), cleaning, spraying, coating, etc. using a treatment liquid on the deodorant object in advance, and then drying, etc. to attach the component (a) to the surface of the object. After this, the concept includes the act of newly adhering microorganisms to the surface of the object, dissolving the component (a) on the surface of the object through water such as urine and sweat, and bringing it into contact with the newly adhering microorganisms. (A) It suffices if the component and the microorganism can come into contact with each other.

<Step 2>
In step 2, the hydrogen peroxide obtained in step 1 is reacted with the iron (II) ion derived from the microorganism to generate hydroxyl radical in the microorganism body, and the odorous compound or the odorous compound produced by the microorganism is produced. It is a step of decomposing or inactivating enzymes involved in production.

In step 2, the hydrogen peroxide obtained in step 1 and the iron (II) ion derived from the microorganism cause a Fenton reaction in the microorganism to generate hydroxyl radical. This hydroxyl radical deodorizes the odorous compound produced by the microorganism or the enzyme involved in the production of the odorous compound by decomposing or inactivating it. Enzymes involved in the production of odorous compounds produced by microorganisms react with odorous compound precursors to generate odorous compounds.

In the present invention, the "iron (II) ion derived from a microorganism" is generated by reducing the iron (III) ion present in the protein structure in the microbial body of step 1 of the present invention by the component (a). Iron (II) ion, iron (III) ion generated by oxidation of iron (II) ion in the hydroxy radical generation reaction in step 2 of the present invention is further present in the vicinity (a) component reduced iron (II) ) Ions and iron (II) ions taken into the body by microorganisms from the environment.

The odorous compounds produced by microorganisms include phenols such as p-cresol, indols such as skatole, fatty acids such as 3-hydroxy-3-methylhexanoic acid and 3-methyl-2-hexenoic acid, and 3-mercapto-. Examples thereof include one or more selected from thioalcohols such as 3-methylskatole, amines such as ammonia, and steroids such as androstenone.
Enzymes involved in the production of odorous compounds produced by microorganisms include one or more selected from β-glucuronidase, lipase, aminoacylase, β-riase, urease, and arylsulfatase.

Steps 1 and 2 are carried out at the same time by bringing the treatment liquid of the present invention into contact with the microorganism and taking the component (a) into the microorganism. That is, in the deodorizing method of the present invention, step 1 and step 2 may be performed at the same time.
As a method of bringing the treatment liquid of the present invention into contact with microorganisms, when the treatment liquid of the present invention is brought into contact with microorganisms, the treatment liquid of the present invention and water are mixed to prepare a washing liquid, and the washing liquid is used to wash the textile product in a washing machine. A method of cleaning with or the like can be mentioned. The washed textile products are washed and then dried. In this case, steps 1 and 2 are performed during or after washing (during drying or after drying).

Examples of the method of contacting the treatment liquid of the present invention with microorganisms include a method of spraying or applying the treatment liquid of the present invention to the textile product when the treatment liquid of the present invention is applied to the microorganisms present in the textile product. Further, the method of contacting the treatment liquid of the present invention with a microorganism may be a method of spraying or applying the treatment liquid of the present invention to a textile product and bringing the treatment liquid of the present invention into contact with the textile product when the microorganism newly adheres to the textile product. The method of spraying or applying the treatment liquid to the textile product is to fill a container with a sprayer with the treatment liquid of the present invention and spray the treatment liquid on the textile product to bring the treatment liquid into contact with the textile product. The method is preferred. Further, the treatment liquid of the present invention may be brought into contact with the textile product by applying the treatment liquid of the present invention to the textile product with a coating tool such as a cloth or a brush. Step 1 and step 2 are performed by spraying or applying the treatment liquid of the present invention to the textile product.

The deodorizing method of the present invention is a textile product capable of washing clothes, towels, bedding, textile products for bedding (sheets, pillowcases, etc.) when the treatment liquid of the present invention is used as a cleaning agent composition for washing. Used for cleaning.
Further, the deodorizing method of the present invention comprises spraying or applying the treatment liquid of the present invention to textile products, such as clothing such as suits, sweaters, skirts and coats, fabrics such as curtains, interior fabrics such as carpets and sofas, and cars. It is used to deodorize textile products that are difficult to wash when washing seats and the like.

Further, the deodorizing method of the present invention is a non-woven fabric for disposable use, such as disposable diapers, sanitary products, non-woven fabric masks, non-woven fabric sheets, pet sheets, etc., when the treatment liquid of the present invention is sprayed or applied to the non-woven fabric product in advance. It is also used to deodorize products.

<Treatment liquid>
The treatment liquid of the present invention contains the component (a).
The component (a) is one or more compounds selected from ascorbic acid and an ascorbic acid derivative, and at 20 ° C. when all the components (a) are dissolved in an equal amount of a mixture of n-octanol and water. Distribution ratio S (Oc) / S (H ₂ O) of the molar concentration S (Oc) in the n-octanol phase and the molar concentration S (H ₂ O) in the aqueous phase based on the ascorbic acid skeleton, or ( a) Distribution ratio S (Oc) of the molar concentration S (Oc) in the n-octanol phase and the molar concentration S (H ₂ O) in the aqueous phase based on the ascorbic acid skeleton calculated from the logP value of the component. A compound having S (H ₂ O) of 0.0004 or more and 7,000 or less is preferable.

(A) The distribution ratio S (Oc) / S ( _H2O ) of the components improves deodorization by suppressing the generation of odorous compounds derived from the metabolism of microorganisms (hereinafter referred to as deodorant properties) and inhibits the activity of odorous compound-producing enzymes. From the viewpoint of (hereinafter referred to as inhibition of enzyme activity), 0.0004 or more, preferably 0.01 or more, more preferably 0.1 or more, still more preferably 0.3 or more, still more preferably 1 or more, still more preferable. Is 10 or more, and 7000 or less, preferably 1000 or less, more preferably 100 or less, still more preferably 50 or less, still more preferably 25 or less.

The distribution ratio S (Oc) / S (H ₂ O) of the molar concentration S (Oc) in the n-octanol phase and the molar concentration S (H ₂ O) in the aqueous phase of the component (a) is ascorbic acid and ascorbin. A method for quantifying ascorbic acid groups present in the n-octanol phase and the aqueous phase after dissolving one or more compounds selected from acid derivatives in a mixed solution of n-octanol and water at 20 ° C. It can be determined by any of the methods calculated from the logP value of one or more compounds selected from ascorbic acid and ascorbic acid derivatives.

[Method of calculating from the quantification of ascorbic acid groups present in the n-octanol phase and the aqueous phase]
(A) The distribution ratio S (Oc) / S (H ₂ O) of the components can be measured by a method based on JIS Z7260-107. Specifically, at 20 ° C., all the components (a) are dissolved in an equal amount of a mixture of n-octanol and water so as to be 100 mg / L, and the test container is shaken by a shaker or a hand (5). Approximately 100 times per minute). After soaking, the phase is separated by centrifugation, and the n-octanol phase and the aqueous phase are sampled and the concentration is measured by high performance liquid chromatography (HPLC) or the like. For example, the concentration of the component (a) can be measured by HPLC under the following conditions using UV detection at 255 nm.
Eluent A: 1% v / v phosphoric acid aqueous solution (pH 3.0)
Eluent B: Methanol / acetonitrile = 1: 1 (v / v) mixture / gradient condition: 35% eluent B (0-5 minutes) → 35% -45% eluent B (5-12 minutes) → 40- 90% eluent B (12 to 15 minutes), flow rate: 1.5 mL / min, sample injection volume: 20 μL, column: C18-ODS column

[Method of calculating from logP values of ascorbic acid and ascorbic acid derivatives]
(A) The distribution ratio S (Oc) / S (H ₂ O) of the component is calculated by using the following formula for one or more compounds selected from ascorbic acid and ascorbic acid derivatives.

In the formula, C _i is the molar concentration in all the components (a) of the compound i, and LogP _i is the Crippen's fragmentation method (J.Chem.Inf.Comput.Sci., 27,21 (1987)). LogP of compound i calculated from ChemDraw Professional 17.1.

Examples of the ascorbic acid derivative of the component (a) include one or more selected from ascorbic acid fatty acid ester, ascorbic acid phosphate ester, ascorbic acid sulfate ester, and ascorbic acid glycoside, and specific examples thereof include ethyl ascorbin. Acid, Ascorbyl caprylate, Ascorbyl laurate, Ascorbyl palmitate, Ascorbyl isopalmitate, Ascorbyl dipalmitate, Ascorbyl diisopalmitate, Ascorbyl stearate, Ascorbyl isostearate, Ascorbyl disstearate, Ascorbyl diisostearate, Ascorbyl myristate, Ascorbyl isomilystinate, ascorbyl dimyristinate, ascorbyl diisomyristinate, ascorbyl 2-ethylhexanate, ascorbyl di2-ethylhexanate, ascorbyl oleate, ascorbyl dioleate, ascorbyl tetrahexyldecanoate, glyceryl ascorbic acid ester, capri Ascorbic acid alkyl fatty acid ester such as lyl ascorbic acid-2-glyceryl ester, ascorbic acid-2-phosphate ester, ascorbic acid-3-phosphate ester, DL-α-tocopherol-2-ascorbic acid phosphate diester, palmityl Ascorbic acid phosphate such as ascorbic acid-2 phosphate, ascorbic acid sulfate such as ascorbic acid-2-sulfate, ascorbic acid-3-sulfate, ascorbic acid glycoside such as ascorbic acid-2-glucoside Etc., and one or more of these can be used. As the ascorbic acid derivative of the component (a), these salts can also be used, and alkali metal salts such as sodium salt and potassium salt, and alkaline earth metal salts such as calcium salt and magnesium salt are preferably used. However, when these ascorbic acid derivatives are used, it is preferable that the distribution ratio S (Oc) / S (H ₂ O) of the component (a) satisfies the above range. Specifically, the distribution ratio S (Oc) / S ( _H2O ) of the component (a) may satisfy the above range by using ascorbic acid and two or more specific ascorbic acid derivatives in combination. can.

The component (a) is preferably one or more selected from ascorbic acid fatty acid esters from the viewpoint of deodorization and inhibition of enzyme activity. From the viewpoint of deodorization and inhibition of enzyme activity, the carbon number of the raw material fatty acid of the ascorbic acid fatty acid ester is preferably 6 or more, more preferably 8 or more, still more preferably 10 or more, still more preferably 12 or more, and preferably. It is 26 or less, more preferably 20 or less, still more preferably 18 or less, still more preferably 16 or less, still more preferably 14 or less. Examples of the ascorbic acid fatty acid ester include monoesters, diesters, and triesters, and monoesters are preferable.

When the component (a) is one kind of ascorbic acid fatty acid ester, the carbon number of the raw material fatty acid is preferably 6 or more, more preferably 8 or more, still more preferably 10 from the viewpoint of deodorization and inhibition of enzyme activity. The above is even more preferably 12 or more, preferably 26 or less, more preferably 20 or less, still more preferably 18 or less, still more preferably 16 or less, and even more preferably 14 or less.

When the component (a) is used in combination from two or more kinds of ascorbic acid and ascorbic acid fatty acid ester, the distribution ratio S (Oc) / S ( _H2O ) in the whole component (a) satisfies the above range. Is preferable, and there is no particular limitation on the combination. (A) When the component is used in combination from two kinds of ascorbic acid and ascorbic acid fatty acid ester, the distribution ratio S (Oc) / S ( _H2O ) of one ascorbic acid and ascorbic acid fatty acid ester is deodorant and enzyme. From the viewpoint of activity inhibition, it is preferably 0.0004 or more, preferably 1 or less, more preferably 0.5 or less, still more preferably 0.1 or less, and specifically, carbon of ascorbic acid or a raw material fatty acid. It is an ascorbic acid fatty acid ester having a number of 2 or more and 10 or less, and ascorbic acid is preferable from the viewpoint of deodorizing property and inhibition of enzyme activity. The distribution ratio S (Oc) / S ( _H2O ) of the other ascorbic acid fatty acid ester is preferably 10 or more, more preferably 100 or more, still more preferably 500 or more, and from the viewpoint of deodorization and inhibition of enzyme activity. It is preferably 8000 or less, more preferably 1000 or less, and specifically, the raw material fatty acid has a carbon number of preferably 12 or more, more preferably 14 or more, and preferably 14 or more from the viewpoint of deodorizing property and inhibition of enzyme activity. Is 20 or less, more preferably 18 or less, still more preferably 16 or less.

The addition amount ratio of the two types of ascorbic acid and the ascorbic acid fatty acid ester is the molar concentration S (Oc) in the n-octanol phase calculated by averaging the respective distribution ratios S (Oc) / S ( _H2O ). The value of the distribution ratio S (Oc) / S (H ₂ O) of the molar concentration S (H ₂ O) in the aqueous phase is 0.0004 or more, preferably 0. 01 or more, more preferably 0.1 or more, still more preferably 0.3 or more, still more preferably 1 or more, still more preferably 10 or more, and 7000 or less, preferably 1000 or less, more preferably 100 or less, further. It is preferably prepared so as to be preferably 50 or less, and even more preferably 25 or less.

In the case of the aspect in which the component (a) of the present invention is used for the purpose of treating the object in water, the effect varies when the composition of the component (a) adsorbed on the surface of the object fluctuates. Therefore, it is preferable to compose the treatment liquid of the present invention with one kind of ascorbic acid fatty acid ester. Further, in the case of a usage mode in which the treatment liquid of the present invention is sprayed or applied to the surface of the object by spraying or the like, all the components (a) are fixed on the surface of the object, so that one kind of ascorbic acid fatty acid. It may be an ester or an ascorbic acid and an ascorbic acid fatty acid ester selected from two or more kinds.

The treatment liquid of the present invention can contain a surfactant as the component (b). In the deodorizing method of the present invention, an aqueous solution of the component (a) is often used as the treatment liquid, but depending on the structure of the component (a), some have low solubility in water and some have poor dispersibility, and are uniform. It causes a decrease in efficiency such as treatment and transfer to the component (a) into the body of the microorganism. Therefore, in order to improve the solubility of the component (a) in the treatment liquid and the uptake of the component (a) into the microorganism, further, the deodorizing effect of the present invention is improved, and value other than deodorization such as detergency is added. Therefore, it is preferable that the treatment liquid of the present invention contains the component (b).
The surfactant is not particularly limited as long as it does not adversely affect the progress of steps 1 and 2 of the present invention and the deodorizing effect of the component (a). , (B2) cationic surfactant (hereinafter, also referred to as (b2) component), (b3) nonionic surfactant (hereinafter, also referred to as (b3) component), and (b4) amphoteric surfactant (b4). Hereinafter, one or more selected from (b4) component) may be mentioned.

(B1) The anionic surfactant includes one or more hydrocarbon groups having 8 or more and 18 or less carbon atoms, and one or more groups selected from the group consisting of a sulfonic acid group, a sulfate ester group and a carboxylic acid group. Examples thereof include anionic surfactants having. Examples of the anionic surfactant include alkyl or alkenylbenzenesulfonic acid having 8 or more and 18 or less carbon atoms or a salt thereof, polyoxyalkylene alkyl or alkenyl ether sulfate ester having 8 or more carbon atoms or 18 or less carbon atoms or a salt thereof, and having 8 or more carbon atoms and 18 or less carbon atoms. Examples thereof include the following alkyl or alkenyl sulfate esters or salts thereof, internal olefin sulfonic acids having 8 or more carbon atoms or 18 or less carbon atoms or salts thereof, fatty acids or salts thereof, and the like.
The salt of the anionic surfactant is preferably an alkali metal salt such as a sodium salt or a potassium salt.

(B2) Examples of the cationic surfactant include a quaternary ammonium salt-type cationic surfactant. As the quaternary ammonium salt type cationic surfactant, one or two of the groups bonded to the nitrogen atom are hydrocarbons having 6 or more carbon atoms, preferably 8 or more carbon atoms, and 26 or less, preferably 18 or less carbon atoms. It is a quaternary ammonium salt type cation which is a group selected from the group consisting of an alkyl group having 1 or more and 3 or less carbon atoms, a hydroxyalkyl group having 1 or more and 3 or less carbon atoms, and an arylalkyl group (benzyl group, etc.). Examples include ionic surfactants. When it is desired to add bactericidal performance to the treatment liquid, the component (b2) is preferably a quaternary ammonium salt-type cationic surfactant having a benzyl group from the viewpoint of bactericidal performance.

(B3) Examples of the nonionic surfactant include a polyoxyalkylene alkyl ether having an alkyl group having 8 or more and 18 or less carbon atoms, a polyoxyalkylene alkenyl ether having an alkenyl group having 8 or more and 18 or less carbon atoms, and 18 or more carbon atoms. Polyoxyalkylene sorbitan fatty acid ester having the following fatty acid groups, alkyl glycoside having an alkyl group having 8 or more and 18 or less carbon atoms, alkyl polyglycoside having an alkyl group having 8 or more and 18 or less carbon atoms, fatty acid having 8 or more and 18 or less carbon atoms. Examples thereof include sucrose fatty acid esters having a group, alkyl polyglyceryl ethers having an alkyl group having 8 or more and 18 or less carbon atoms, and one or more of these can be used. The component (b3) is preferably a polyoxyethylene alkyl ether having an alkyl group having 8 or more and 18 or less carbon atoms and having an average number of moles of ethylene oxide added of 2 or more and 50 or less.

The component (b3) is preferably a nonionic surfactant represented by the following general formula (b3).
R ^31b -O- [(C ₂ H ₄ O) _s (C ₃ H ₆ O) _t ] -H (b 3)
[In the formula, R ^31b is an alkyl group or alkenyl group having 8 or more carbon atoms, preferably 10 or more, and preferably 18 or less, preferably 16 or less, and is a residue derived from a primary alcohol or a secondary alcohol. It's okay. s and t are average addition moles, where s is 2 or more, preferably 4 or more, more preferably 6 or more, still more preferably 10 or more, and 50 or less, preferably 40 or less, more preferably 20 or less. The number, t is 0 or more, preferably 1 or more, and 5 or less, preferably 3 or less, and t may be 0. (C ₂ H ₄ O) and (C ₃ H ₆ O) may be a random polymer or a block polymer. ]

(B4) Examples of the amphoteric surfactant include one or more surfactants selected from betaine-type surfactants and amine oxide-type surfactants. Specific examples of the component (b4) include one or more surfactants selected from sulfobetaine, carbobetaine and amine oxide.

Examples of the sulfobetaine include N-alkyl-N, N-dimethyl-N-sulfopropylammonium sulfobetaine having an alkyl group having 10 or more and 18 or less carbon atoms, and N- having an alkyl group having 10 or more and 18 or less carbon atoms. Alkyl-N, N-dimethyl-N- (2-hydroxysulfopropyl) ammonium sulfobetaine, N-alkanoylaminopropyl-N, N-dimethyl-N-sulfopropylammoniumsulfone having 10 or more and 18 or less carbon atoms in the alkanoyl group. Examples thereof include betaine and N-alkanoylaminopropyl-N, N-dimethyl-N- (2-hydroxysulfopropyl) ammonium sulfobetaine having 10 or more and 18 or less carbon atoms in the alkanoyl group.

Examples of the carbobetaine include N-alkyl-N, N-dimethyl-N-carboxymethylammonium betaine having an alkyl group having 10 or more and 18 or less carbon atoms and a compound represented by the following general formula (b41).

[In the formula, R ^41b represents an alkyl group or an alkenyl group having 7 or more and 21 or less carbon atoms, R ^42b represents a propylene group, and R ^43b and R ^44b each independently represent an alkyl group having 1 or more and 3 or less carbon atoms. Is shown. ]

As the amine oxide, the compound of the following general formula (b42) is suitable.

[In the formula, R ^45b represents a hydrocarbon group having 7 or more and 22 or less carbon atoms, preferably an alkyl group or an alkenyl group, more preferably an alkyl group, and R ^46b and R ^47b are the same or different and have 1 or more carbon atoms. Shows 3 or less alkyl groups. D represents an -NHC (= O) -group or a -C (= O) NH- group, and E represents an alkylene group having 1 or more and 5 or less carbon atoms. m and p indicate m = 0 and p = 0 or m = 1 and p = 1. ]

The treatment liquid of the present invention can be a composite type treatment liquid having other functions by blending other compounds as long as the deodorizing effect obtained by the deodorization method of the present invention is not impaired. Specific examples thereof include a deodorant detergent composition for textile products, a softener composition for textile products, a deodorant composition for spray-type textile products, a deodorant antistatic agent for textile products, and the like.

The treatment liquid of the present invention may contain other optional components depending on the purpose in order to prepare a composite type treatment liquid having other functions. Specifically, soft base materials, alkaline agents, chelating agents, recontamination inhibitors, polymer dispersants, bleaching agents, bleaching activators, enzymes, fluorescent dyes, antioxidants, pigments, fragrances, antibacterial preservatives, Examples thereof include a defoaming agent such as silicone, an organic solvent having a hydroxyl group, a hydrotropic agent, and a deodorizing base material. However, those corresponding to the component (a) and the component (b) are excluded from these optional components.

The treatment liquid of the present invention contains the component (a) in an amount of preferably 0.001% by mass or more, more preferably 0.01% by mass or more, still more preferably 0.1% by mass, from the viewpoint of deodorizing property and inhibition of enzyme activity. As described above, the content is more preferably 1.0% by mass or more, preferably 10% by mass or less, more preferably 8% by mass or less, still more preferably 5% by mass or less. Further, the treatment liquid of the present invention is preferably 0.0001 mol% or more, more preferably 0.0005 mol% or more, still more preferably 0.001 mol% or more, and preferably 0.001 mol% or more, from the viewpoint of deodorizing property and inhibition of enzyme activity. Is contained in an amount of 0.3 mol% or less, more preferably 0.1 mol% or less, still more preferably 0.05 mol% or less.

In the treatment liquid of the present invention, the component (b) is preferably 0.001% by mass or more, more preferably 0.01% by mass or more, from the viewpoint of ensuring the performance as a fiber treatment agent, deodorizing property and inhibiting enzyme activity. More preferably 0.1% by mass or more, still more preferably 1% by mass or more, still more preferably 5.0% by mass or more, and preferably 30% by mass or less, more preferably 20% by mass or less, still more preferably. Contains 15% by mass or less.

In the treatment liquid of the present invention, the mass ratio (a) / (b) of the content of the component (a) to the content of the component (b) is preferably 0.01 from the viewpoint of deodorization and inhibition of enzyme activity. More preferably 0.05 or more, still more preferably 0.1 or more, still more preferably 0.2 or more, and preferably 0.8 or less, more preferably 0.6 or less, still more preferably 0.5. Below, it is even more preferably 0.3 or less.

The treatment liquid of the present invention contains water. As the water, ion-exchanged water, distilled water, tap water, water containing 1 mg / kg or more and 5 mg / kg or less of sodium hypochlorite can be used. In the treatment liquid of the present invention, water may be used as the balance of the components constituting the treatment liquid, but specifically, it is preferably 50% by mass or more, more preferably 70% by mass or more, and further preferably 80% by mass. The above, preferably 98% by mass or less, more preferably 95% by mass or less, still more preferably 92% by mass or less.

The treatment liquid of the present invention can be appropriately set depending on the purpose, but the pH at 25 ° C. is preferably 3 or more, more preferably 4 or more, and preferably 12 or less from the viewpoint of deodorization and inhibition of enzyme activity. More preferably, it is 11 or less. The pH is a value measured at 25 ° C. using a glass electrode. Specifically, it was measured by the following method.
<pH measurement method>
HORIBA, Ltd. pH meter D-52 is calibrated with a pH electrode (model 6637) in advance with a phthalic acid buffer solution (pH 4.01), a phosphoric acid standard solution (pH 6.84), and a borate standard solution (pH 9.18). Rinse thoroughly with ion-exchanged water. Place the pH electrode calibrated and washed as described above in the treatment liquid whose temperature has been adjusted to 25 ° C., and measure until the measured value becomes constant using the AUTO HOLD mode of the pH meter.

The viscosity of the treatment liquid of the present invention at 25 ° C. is preferably 15 mPa · s or less, more preferably 10 mPa · s or less, still more preferably 5 mPa · s or less, from the viewpoint of sprayability in a container equipped with a sprayer. , And more preferably 1 mPa · s or more, more preferably 1.5 mPa · s or more, still more preferably 2 mPa · s or more.
The viscosity of the treatment liquid was No. 1 in a B-type viscometer (model type BM) manufactured by Tokyo Keiki Co., Ltd. Attach the rotor of 1, fill the deodorant composition in a glass tall beaker with a capacity of 200 mL, adjust to 25 ± 0.3 ° C in a water bath, set the rotation speed of the rotor to 60 r / min, and measure. It is an indicated value 60 seconds after the start.

When the deodorizing method of the present invention is, for example, a method in which the treatment liquid of the present invention and water are mixed to prepare a cleaning liquid, and the textile product is washed with the cleaning liquid in a washing machine or the like, the component (a) in the cleaning liquid is used. The content of is preferably 0.00001% by mass or more, more preferably 0.0001% by mass or more, further preferably 0.001% by mass or more, and preferably 5% by mass from the viewpoint of deodorizing property and inhibition of enzyme activity. % Or less, more preferably 3% by mass or less.
When the component (b) is treated as a cleaning component, the content of the component (b) in the cleaning liquid is preferably 0.00001% by mass or more, more preferably 0, from the viewpoint of detergency, deodorant property and inhibition of enzyme activity. It is .0001% by mass or more, more preferably 0.001% by mass or more, and preferably 15% by mass or less, more preferably 12% by mass or less.
The mass ratio (a) / (b) of the content of the component (a) to the content of the component (b) in the cleaning liquid is preferably 0.01 or more from the viewpoint of deodorizing property and inhibition of enzyme activity. It is preferably 0.05 or more, more preferably 0.1 or more, still more preferably 0.2 or more, and preferably 0.8 or less, more preferably 0.6 or less, still more preferably 0.5 or less. More preferably, it is 0.3 or less.

The cleaning liquid is preferably prepared by diluting the treatment liquid of the present invention with water so that the content of each component is within this range. The specific dilution ratio is preferably 5 times or more, more preferably 50 times or more, still more preferably 500 times or more, and more, from the viewpoint of the form such as the viscosity of the treatment liquid of the present invention and workability at the time of washing clothes. It may be more preferably 800 times or more, preferably 5000 times or less, and more preferably 3000 times or less.

When the deodorizing method of the present invention is a method of preparing a cleaning liquid by mixing the treatment liquid of the present invention and water and washing the textile product with the cleaning liquid in a washing machine or the like, the mass (kg) of the textile product and the cleaning liquid The value of the bath ratio expressed by the ratio of the amount (liter), that is, the value of the amount of the washing liquid (liter) / the mass of the clothes (kg) (hereinafter, this ratio may be referred to as the bath ratio) determines the detergency. From the viewpoint of securing, it is preferably 2 or more, more preferably 3 or more, still more preferably 4 or more, still more preferably 5 or more, and preferably 400 or less, more preferably 300 or less.

When the deodorizing method of the present invention is a method of preparing a washing liquid by mixing the treatment liquid of the present invention and water and washing the textile product with the washing liquid in a washing machine or the like, the time for washing the textile product is detergency. From the viewpoint of ensuring, preferably 1 minute or more, more preferably 2 minutes or more, further preferably 3 minutes or more, and preferably 12 hours or less, more preferably 8 hours or less, still more preferably 6 hours or less, still more. It is preferably 3 hours or less, and even more preferably 1 hour or less.
After washing the textiles, rinse the garment with water. In rinsing, rinse water can be used for textile products at a ratio similar to the bath ratio of the cleaning liquid. Further, the rinsing time can be in the same range as the washing time.

When the deodorizing method of the present invention is a method of spraying or applying the treatment liquid of the present invention to a textile product to deodorize, the method of spraying or applying the treatment liquid of the present invention to a textile product is described in a container with a sprayer. A method in which the treatment liquid of the present invention is filled and the treatment liquid is sprayed onto the textile product to bring it into contact with the textile product is preferable. Further, the treatment liquid of the present invention may be applied to the textile product with a coating tool such as a cloth or a brush to bring it into contact with the textile product.

The following evaluation was performed using the following components.

<Ingredients>
(A) Ingredients-Ascorbyl caprylate: Synthetic product, LogP-0.39
-Ascorbyl laurate: Synthetic product, LogP 1.28
・ Ascorbyl palmitate: Tokyo Kasei, LogP 2.95
・ Ascorbyl stearate: Tokyo Kasei, LogP 3.79
・ Ascorbic acid: Tokyo Kasei, LogP-3.36

Ascorbyl caprylate and ascorbyl laurate were synthesized according to J Am Oil ChemSoc54: 308- 312 (1977).

<Synthesis of ascorbyl laurate>
L-ascorbic acid (8.0 mmol) and lauric acid (10 mmol) were dissolved in concentrated sulfuric acid (25 mL), and the mixture was stirred at 25 ° C. for 24 hours to carry out an esterification reaction. The reaction solution was poured into ice water (150 mL), extracted with diethyl ether, and then the solvent was distilled off by an evaporator to obtain a crude product. The obtained crude product was purified by washing with hexane. The reaction process and compound identification were performed by measuring TLC and NMR spectra, and it was confirmed that the purity was 98% or higher.

<Synthesis of ascorbyl caprylate>
Caprylic acid ascorbyl was synthesized in the same manner as the method for synthesizing ascorbyl laurate except that lauric acid was changed to caprylic acid. The reaction process and compound identification were performed by measuring TLC and NMR spectra, and it was confirmed that the purity was 98% or higher.

Molar concentration S in the n-octanol phase based on the ascorbic acid skeleton at 20 ° C. when the component (a) of each test solution in Tables 1 to 4 is dissolved in an equal amount of a mixture of n-octanol and water ( S (Oc) / S (H ₂ O), which is the distribution ratio of the molar concentration S (H ₂ O) between Oc) and the aqueous phase, was calculated using the following equation.

In the formula, C _i is the molar concentration in all the components (a) of each compound i, and LogP _i uses the Crippen's fragmentation method (J.Chem.Inf.Comput.Sci., 27,21 (1987)). It is the LogP of each compound i calculated from ChemDraw Professional 17.1.

(B) Ingredients-Nonionic surfactant 1: Polyoxyalkylene alkyl ether with an average of 9 ethylene oxides, an average of 2 propylene oxides, and an average of 9 ethylene oxides added to lauryl alcohol.-Nonionic surfactant 2: 12 to 12 carbon atoms. Polyoxyalkylene alkyl ether (Sophanol 70H, manufactured by Nippon Catalyst Co., Ltd.) in which an average of 7 mol of ethylene oxide is added to 14 secondary alcohols.
-Cationic Surfactant 1: N, N-didecil-N-ethyl-N-methylammonium sulfate ethyl salt (Cotamin D10-ES, manufactured by Kao Corporation)
-Cationic surfactant 2: N-ethyl-N, N dimethyltetradecylammonium sulfate ethyl salt (Cotamin 40ES, manufactured by Kao Corporation)
Polyooxyethylene (10) lauryl ether: In the general formula (b3), R ^31b is an alkyl group having 12 carbon atoms, s is 10 and t is 0.

Other ingredients ・ Ferritin: Ferritin (derived from horse spleen) (100 mg / 1 mL) (manufactured by Tokyo Kasei Kogyo Co., Ltd.), model protein containing iron (III) ion ・ BSA: albumin, bovine serum (BSA), protease-free Includes (manufactured by Fujifilm Wako Junyaku Co., Ltd.), model protein that does not possess iron (III) ions ・ DPBS: Dulbecco`s Phosphate-BufferedSaline (DPBS, calcium, magnesium, Thermo Fisher Scientific)
0.1M Phosphate Buffer (pH 6.0): 0.1 mol / L Phosphate Buffer, pH 6.0 (manufactured by Wako Pure Chemical Industries, Ltd.)

<(A) Confirmation of the location of the component>
The test solutions shown in Table 1 were prepared and allowed to stand at 25 ° C. for 2 hours. After standing, ferritin was removed by ultrafiltration using Amicon Ultra 100K Devices. The filtrate was diluted 10-fold with methanol to prepare a measurement solution. Next, the component (a) used was diluted with methanol for each component to prepare 0.1 ppm, 0.5 ppm, 1.0 ppm, 5.0 ppm, and 10.0 ppm calibration curve solutions, respectively. The prepared calibration curve solution was quantified with a liquid chromatograph mass spectrometer (hereinafter abbreviated as LCMS device) under the following conditions to prepare a calibration curve. Next, the measurement solution was quantified by an LCMS apparatus, and the concentration of the component (a) of the measurement solution (aqueous phase) was determined from the calibration curve. Then, the concentration of the component (a) in ferritin was calculated from the following formula. The results are shown in Table 1.
(A) component concentration in ferritin = (total concentration of (a) component in the test solution (25 ppm))-(concentration of (a) component in the measurement solution)

-LCMS device: LCMS2020 manufactured by Shimadzu Corporation (ESI detection)
・ Measurement mode: SIM
-Measured ion: ascorbic acid: m / z (-) = 175.0, caprylic acid ascobyl: m / z (-) = 301.0, laurate ascobyl: m / z (-) = 357.0, myristic acid Ascovir: m / z (-) = 413.2, ascorbyl stearate: m / z (-) = 441.2
-Column: Imtakt Unison UK-C18 HT (50 * 2 mm) 3 μm
Eluent A: 0.1% formic acid aqueous solution Eluent B: Methanol: Acetonitrile = 1: 1
-Gradient conditions: 50% eluent B (0-2 minutes) → 50% -90% eluent B (2-3.3 minutes) → 90% eluent B (3.3-5.3 minutes) → 90 % -50% eluent B (5.3-6.0 minutes) → 90% eluent B (6.0-8.0 minutes), flow rate: 0.6 mL / min, sample injection volume 5 μl, column temperature 40 ℃

As shown in Table 1, it can be seen that the component (a) is distributed in a specific ratio between the structure formed by ferritin, which is a hydrophobic site in the test solution, and the aqueous phase. The distribution ratio does not necessarily match the distribution ratio S (Oc) / S ( _H2O ) value used as an index of the present invention because the hydrophobicity differs between ferritin and n-octanol, but the distribution ratio S (Oc) Since the tendency matches between the / S (H ₂ O) value and the measured distribution rate with ferritin, the distribution ratio S (Oc) / S (H ₂ O) value is the component (a) in the cell. It is a measure of the distribution behavior to hydrophobic and hydrophilic sites.
Ferritin is a model of a protein structure containing iron (III) ions that form a hydrophobic site in a microorganism, and the prepared test solution has a phosphate buffer atmosphere and is hydrophilic in the microorganism. It becomes a model of the site and a model of the inside of the microbial cell as a whole.

<Analysis of hydrogen peroxide production (step 1)>
The test liquids shown in Table 2 were prepared using the above components. A fluorescent probe BES-H2O2-Ac (manufactured by Wako Pure Chemical Industries, Ltd.) was added to the prepared test solution to 20 μM for detection of hydrogen peroxide, and the mixture was incubated at 25 ° C. for 30 minutes. The fluorescence intensity (Ex / Em: 485/515) of the reaction solution was measured using a microplate reader Infinite (R) 200PRO (Tecan). The fluorescence intensity ratio when Comparative Example 1 was set to 1 was calculated as the amount of hydrogen peroxide generated. The results are shown in Table 2.
The fluorescent probe immediately reacts with hydrogen peroxide to emit fluorescence. Since decomposition of hydrogen peroxide and other reactions do not occur, the hydrogen peroxide production process of step 1 is shown.

<Analysis of iron (II) ion production (step 1)>
The test liquids shown in Table 2 were prepared using the above components. The test solution was incubated at 37 ° C. for 24 hours. For the detection of iron (II) ions, 1 μL of a fluorescent probe Ferrorange (Dojin Kagaku) 1 mM DMSO solution was added to 500 μL of the test solution and reacted at 25 ° C. for 30 minutes, and then using a microplate reader Infinite ^TM 200PRO (Tecan). The fluorescence intensity of the reaction solution (Ex / Em: 543/580) was measured. The fluorescence intensity ratio when Comparative Example 1 was set to 1 was calculated as the amount of iron (II) ion generated. The fluorescent probe specifically reacts with iron (II) ions to emit fluorescence.

In Table 2, in Examples 6 to 10 in which the component (a) was reacted with ferritin, which is a model protein containing iron (III) ions, Comparative Example 1 in which the component (a) was not contained was reacted. It can be seen that hydrogen peroxide is produced as compared with 2. On the other hand, in Comparative Example 3 in which the component (a) was reacted with BSA, which is a model protein that does not possess the iron (III) ion constituting the microorganism, no hydrogen peroxide was observed. Further, in Examples 6 to 10, it can be seen that the reaction of producing iron (II) ion by the component (a) and the iron (III) ion is carried out at the same time as the production of hydrogen peroxide.
Ferritin is a model of a protein structure containing iron (III) ions that form a hydrophobic site in a microorganism, and the prepared test solution has a phosphate buffer atmosphere and is hydrophilic in the microorganism. It becomes a model of the site and a model of the inside of the microbial cell as a whole.

<Analysis of hydroxyl radical production (steps 1 and 2)>
The test solution shown in Table 3 was prepared using the above components. A fluorescent probe HPF reagent (Hydroxyphenyl Fluorescein (manufactured by Goryo Chemical Co., Ltd.)) for detecting hydroxyl radicals was added to the prepared test solution to a concentration of 10 μM, and the mixture was incubated at 37 ° C. for 24 hours. Fluorescence intensity (Ex / Em: 492/525) was measured using a microplate reader Infinite (R) 200PRO (Tecan). Using the following formula, the amount of hydroxyl radicals generated was calculated from the fluorescence intensity ratio when Comparative Example 4 was set to 1. The results are shown in Table 3.
Since the fluorescent probe selectively reacts only with hydroxyl radicals and does not react with hydrogen peroxide, the hydroxyl radical production process of step 2 using the hydrogen peroxide generated in step 1 is shown.

In Table 3, in Examples 11 to 16 in which the component (a) was reacted with ferritin, which is a model protein containing iron (III) ions, the reaction was carried out with Comparative Example 4 in which the component (a) was not contained. In comparison, it can be seen that hydroxyl radicals, which are active oxygen, are generated. From the above results and the results in Table 2, it can be said that in Examples 11 to 16, Steps 1 and 2 were performed to generate hydroxyl radicals.
Ferritin is a model of a protein structure containing iron (III) ions that form a hydrophobic site in a microorganism, and the prepared test solution has a phosphate buffer atmosphere and is hydrophilic in the microorganism. It becomes a model of the site and a model of the inside of the microbial cell as a whole.

<Evaluation of deodorant property>
(1) Evaluation of inhibition of enzymes involved in urine odor production 833 ppm of the test solution shown in Table 4 and 100 ppm of ferritin, which is a model protein having iron (III) ions as iron (III) hydroxide in the protein structure, P-nitrophenyl-β-glucuronide 1 mM, which is a precursor of an odor model substance, and β-glucuronidase 0.5 units / mL, which is an enzyme involved in the production of odor compounds produced by microorganisms, are added to a phosphate buffer (pH 6.0). ) And reacted under 37 ° C. conditions for 24 hours.
160 μL of the reaction solution was added to the well of a 96-well plate to which 40 μL of 1M glycine buffer (pH 10.4) was dispensed in advance, and a microplate reader Infinite200PRO (registered trademark, Tecan) was used to obtain p-nitrophenol as an odor model substance. The absorbance at a wavelength of 405 nm, which is a characteristic beak value, was measured. In addition, the case where ion-exchanged water was used instead of the test solution (Comparative Example 2) was used as a control, and the absorbance was measured in the same manner. From the obtained measured values, the relative enzyme activity inhibition rate of β-glucuronidase was calculated using the following formula. Β-Glucronidase, an enzyme involved in the production of odorous compounds produced by β-glucuronidase active bacteria of microorganisms, decomposes p-nitrophenyl-β-glucuronide, which is a precursor of odor model substances, and is an odor model substance. Generates a certain p-nitrophenol. It can be said that the higher the relative enzyme activity inhibition rate of this β-glucuronidase, the more deodorant it is because it suppresses the generation of p-nitrophenol, which is an odor model substance. The results are shown in Table 4.
Ferritin is a model of a protein structure containing iron (III) ions that form hydrophobic sites in the microorganism, and the prepared test solution has a phosphate buffer atmosphere and is hydrophilic in the microorganism. It becomes a model of the site and a model of the inside of the microbial cell as a whole. Further, in this reaction system, it is considered that step 1 is proceeding at a hydrophobic site and step 2 is proceeding at a hydrophilic site at the same time.

Further, p-nitrophenol, which is an odor model substance, is diluted with water to prepare a solution for a calibration curve of 0.05, 0.1, 0.5, and 1 mM, which is also a characteristic beak value of p-nitrophenol. The absorbance at a wavelength of 405 nm was measured and a calibration curve was prepared. From the prepared calibration curve, the amount of p-nitrophenol (nmol / mL) of each reaction solution was calculated. It can be said that the smaller the amount of p-nitrophenol, the better the deodorizing property. The results are shown in Table 4.

(2) Deodorization evaluation by washing treatment The sheets collected from the nursing facility were cut into 6 cm x 6 cm (0.4 g) and used as a test cloth. The cleaning operation was performed using a targotometer (manufactured by Ueshima Seisakusho). The water used for cleaning was tap water from Wakayama City. The test solution shown in Table 4 was mixed with tap water so that the concentration in the cleaning solution was 833 ppm to obtain a cleaning solution. A cleaning solution of 0.6 L and three test cloths were placed in a 1-liter stainless steel beaker for a cleaning test. The temperature of the cleaning liquid was 20 ° C. The test cloth was washed with a turgotometer at 85 rpm for 10 minutes. After washing, dehydration was performed for 1 minute in a two-layer washing machine PS-H35L (Hitachi). The dehydrated test cloth was put into 0.6 L of tap water and rinsed with a turgotometer at 85 rpm for 3 minutes. After rinsing the test cloth and dehydrating it again, 200 μL of a 50 ppm aqueous solution of the urinary odor substance precursor p-cresol-glucuronide was applied as model urine, and the mixture was allowed to stand in a constant temperature bath at 30 ° C. for 6 hours in a closed state. The malodor intensity of the sample cloth after standing was sensory evaluated by 6 panelists according to the 6-step odor intensity display in the following evaluation criteria, and the results of the average values of the 6 subjects are shown in Table 4.
5: Offensive odor is strongly felt 4: Offensive odor is strongly felt 3: Offensive odor is slightly strong 2: Offensive odor is weakly felt 1: Offensive odor is barely perceptible 0: No offensive odor at all

(3) Deodorization evaluation by spray treatment The sheets collected from the nursing facility were cut into 6 cm x 6 cm (0.4 g) and used as a test cloth. After spraying 0.4 g of the test solution shown in Table 5 on the test cloth using spray vial No. 5 (manufactured by Marum), apply 200 μL of a 50 ppm aqueous solution of the urine odor substance precursor p-cresol-glucuronide as model urine and seal it. In this state, it was allowed to stand in a constant temperature bath at 30 ° C. for 6 hours. The malodor intensity of the test cloth after standing was sensory evaluated by 6 panelists according to the 6-step odor intensity display in the following evaluation criteria, and the results of the average values of the 6 subjects are shown in Table 5.
5: Offensive odor is strongly felt 4: Offensive odor is strongly felt 3: Offensive odor is slightly strong 2: Offensive odor is weakly felt 1: Offensive odor is barely perceptible 0: No offensive odor at all

Claims (7)

A method for deodorizing odors derived from microorganisms, which comprises the following steps 1 and 2.
<Process 1>
The treatment liquid containing the following component (a) is brought into contact with the microorganism, and the component (a) is taken into the body of the microorganism in the presence of iron (III) ions present in the protein structure constituting the microorganism in the body of the microorganism. , (A) A step of producing hydrogen peroxide by a component and dissolved oxygen in a microorganism, and a reaction of (a) producing iron (II) ion by a component and iron (III) ion.
(A) Ingredient: One or more compounds selected from ascorbic acid and ascorbic acid derivatives
<Process 2>
In the body of a microorganism, the hydrogen peroxide obtained in step 1 is reacted with iron (II) ions derived from the microorganism to generate hydroxyl radicals, which are involved in the production of odorous compounds or odorous compounds produced by the microorganisms. The step of degrading or inactivating an enzyme. The method for deodorizing a odor derived from a microorganism according to claim 1, wherein step 1 and step 2 are performed at the same time. At 20 ° C., when the component (a) is one or more compounds selected from ascorbic acid and an ascorbic acid derivative and all the components (a) are dissolved in an equal amount of a mixture of n-octanol and water. Distribution ratio S (Oc) / S (H ₂ O) of molar concentration S (Oc) in the n-octanol phase and molar concentration S (H ₂ O) in the aqueous phase based on the ascorbic acid skeleton, or ( a) Distribution ratio S (Oc) of the molar concentration S (Oc) in the n-octanol phase and the molar concentration S (H ₂ O) in the aqueous phase based on the ascorbic acid skeleton calculated from the logP value of the component. The method for deodorizing a odor derived from a microorganism according to claim 1 or 2, wherein S (H ₂ O) is 0.0004 or more and 7000 or less. (A) The method for deodorizing a microorganism-derived odor according to any one of claims 1 to 3, wherein the component is one or more selected from ascorbic acid fatty acid esters. The method for deodorizing a microorganism-derived odor according to any one of claims 1 to 4, wherein the protein in which iron (III) ions are present in the structure is ferritin. The method for deodorizing a microorganism-derived odor according to any one of claims 1 to 5, wherein the steps 1 and 2 are performed on the microorganisms present in the textile product. The method for deodorizing a microorganism-derived odor according to claim 6, wherein steps 1 and 2 are performed during or after washing the textile product.