JP2022080275A - Composition - Google Patents

Abstract

To provide a composition that can achieve a high anti-aging effect.SOLUTION: The inventive composition contains a processed Trapaceae plant and an antioxidant capable of removing active oxygen (particularly, a processed Trapaceae plant and tomato extract). Thus, combining the anti-glycation and anti-carbonylation actions of Trapaceae plants with the removal of active oxygen causing the reaction can suppress saccharification, carbonylation and oxidation, the three main factors of aging, and can exhibit high anti-aging effect.SELECTED DRAWING: Figure 1

Description

The present invention provides a processed product of Trapa japonica and a composition containing an antioxidant.

The saccharification reaction is a reaction in which the carbonyl group of advanced glycation end and the amino group of a protein or amino acid are non-enzymatically condensed. It is further divided into a late reaction that changes to advanced glycation end products (AGEs) through processes such as dehydration, oxidation, condensation, and rearrangement. It is a phenomenon that has long been well known in the field of food science as a browning reaction that occurs during the processing and storage of food.

This reaction also occurs in vivo, and AGEs accumulate in various biological tissues depending on aging, and the accumulation of AGEs is significant in age-related diseases such as diabetic complications, Alzheimer's disease and arteriosclerosis. It has been reported that the number has increased. Furthermore, the glycation reaction causes aging of the skin such as cataracts, decreased cartilage elasticity, wrinkles, sagging and yellowing that occur with aging, and the progress is particularly rapid in diabetic patients who are constantly hyperglycemic. Has been reported.

Therefore, it is considered that a substance that inhibits the glycation reaction can suppress various age-related diseases and diabetic complications in the living body, and various studies have been conducted in search of such a substance.

For example, an extract of a trapa japonica plant obtained by hot-water-extracting the peel of a trapa japonica plant, particularly a trapa japonica extract, is called trapa japonica extract and is known to have a strong inhibitory effect on saccharification (see, for example, Patent Document 1).

Japanese Unexamined Patent Publication No. 2019-23215

However, the conventional compositions having a sugar-suppressing effect cannot be said to have a sufficient anti-aging effect, and a composition having a higher anti-aging effect has been desired.

An object of the present invention is to provide a composition exhibiting a high anti-aging effect.

In order to solve such a problem, the composition of the present invention contains a processed product of Trapa japonica and a tomato extract.

The present invention can realize a composition exhibiting a high anti-aging effect.

It is a schematic diagram explaining the concept of this invention.

As described above, the glycation reaction of proteins causes the accumulation of advanced glycation end products (AGEs) and causes the aging of the living body.

Also, protein carbonylation is one of the major protein damages. It has been confirmed that carbonyl proteins produce lipid peroxidation final products (ALEs: Advanced lipoxidation products) by the addition of aldehyde compounds generated by the oxidative decomposition of amino acid side chains and the peroxidation reaction of lipids.

The stratum corneum, which is located in the outermost layer of the skin, is not only biologically important because it retains water and barriers to transpiration, but it is also a cosmetically important part in the sense that it is actually seen by the eyes. .. The stratum corneum is composed of stratum corneum cells and intercellular lipids, and a protein called keratin is the main component of stratum corneum cells.

In recent years, it has become known that aldehydes such as acrolein, which is a decomposition product of lipid peroxide, are added to proteins to carbonylate the proteins. In pathological conditions such as Alzheimer's disease and arteriosclerosis, which are thought to be involved in oxidation, it is thought that proteins lose their original functions and accumulate due to carbonylation. In addition, in actinic elastosis, an increase in carbonylated protein in the dermis component has been observed. On the other hand, it has been clarified that the carbonylated protein is also present in keratin, the main protein of the stratum corneum, which is easily influenced by the outside world.

Active oxygen is generated in the living body due to various causes such as external factors such as ultraviolet rays and internal factors such as stress. Reactive oxygen species and free radicals are physiologically active substances that are indispensable for maintaining homeostasis of the living body, and at the same time, it is known that when they are present in excess, they have harmful effects on the living body by oxidizing nucleic acids, proteins, and lipids. ing.

Oxidative stress is also closely associated with various skin symptoms, cancer, aging, etc. in the skin.

With the increase in life expectancy and the declining birthrate and aging population, there is growing interest in anti-aging, so-called anti-aging, in order to maintain good health and live longer. The development of highly functional foods and cosmetic materials is eagerly desired.

The present invention focuses on the three actions of saccharification reaction, carbonylation reaction, and active oxygen as anti-aging, and by using a processed product of Trapa plants and a strong antioxidant in combination, three major agings occur. We have found a composition that simultaneously and effectively suppresses the three phenomena called glycation, carbonylation, and oxidation in the living body.

That is, as shown in FIG. 1, the present invention has the effect of suppressing the oxidative reaction itself by suppressing and decomposing the oxidative reaction, and suppressing the blood glucose level to reduce the blood concentration of sugar. It is a composition in which a plant and an antioxidant having an effect of removing active oxygen are combined. As a result, it is possible to suppress the oxidation reaction itself by reducing the amount of active oxygen while suppressing the oxidation reaction, and as a result, a composition that simultaneously and effectively suppresses glycation, carbonylation, and oxidation in the living body is realized. It becomes possible to do. In the figure, the trapa japonica plant is referred to as "trapa japonica" and the antioxidant is referred to as "antioxidant".

The plant of the genus Trapa is not particularly limited, and for example, Trapa japonica, TrapabispinosaRoxb, TrapanatansL.ver.Japonica, Trapaincisa and the like can be preferably used.

The Trapa japonica plant contains components of one or both of the pericarp and the fruit. The extraction method is not limited, but an extract of the active ingredient separated by solvent extraction with an organic solvent or hot water is preferably used.

The fruits and pericarps of Trapaceae plants may be collected from raw fruits or raw fruits, dried after collection, dried fruits or dried fruits, and improve extraction efficiency. Therefore, pretreatment such as crushing or crushing may be performed by any method before solvent extraction.

An extract of a trapa japonica plant obtained by hot water extraction of the peel of a trapa japonica plant, particularly a trapa japonica extract, is called a trapa japonica extract and is known to have a saccharification inhibitory effect. Advanced glycation end products produced by saccharification are also known to be involved in the development of various aging phenomena and diseases by causing denaturation and functional deterioration of biological proteins such as collagen and elastin. By suppressing this saccharification action, it is expected to prevent the aging of the living body, particularly the aging of the skin. Therefore, the Tobishi extract is blended in cosmetics and the like.

Antioxidants include prophylactic antioxidants that scavenge active oxygen and free radicals, and radical-capturing antioxidants that suppress the chain initiation reaction of peroxidation reactions by radicals or inhibit the chain growth reaction of peroxidation reactions. One or both of the oxidizing agents are appropriately selected and used.

The antioxidant is not particularly limited, and known compounds can be used. Preferably, superoxide anion (O2), hydroxy radical (.OH), nitrogen monoxide radical (NO.), Hypochlorite ion (ClO), hydrogen peroxide (H2O2), singlet oxygen (1O2), etc. Low-molecular-weight (molecular weight 100 or less) oxygen compounds that have free radicals or are in a state where free radicals can be easily generated, so-called polyphenols and carotenoids that can remove (revoke) active oxygen, are used.

As the carotenoid, carotenes such as α-carotene, β-carotene, β-cryptoxanthin, lutein, zeaxanthin, and lycopene and xanthophylls are particularly preferably used. In particular, lycopene has the highest activity among all carotenoids including β-carotene for the removal of singlet oxygen, and the antioxidant activity of lycopene is about 3.2 times that of β-carotene. , About 100 times that of vitamin E. When administered orally and absorbed through the intestinal tract, lycopene removes free radicals and protects tissues, cells and DNA from oxidation by free radicals.

The main target organs and tissues of lycopene in the human body are the testis, prostate, liver and intestine. Lycopene is known to effectively reduce the incidence of prostate, uterine and pancreatic cancers over β-carotene.

Previous studies have shown that many functions of lycopene: antiaging, improving immune health, reducing the risk of cardiovascular disease, and malignant tumors (especially oral, pharyngeal, gastric, colon, and colon cancers). It has been shown that the incidence of uterine cancer) is reduced. A comprehensive analysis conducted over 6 years at Harvard University, which includes 47,000 participants, confirms that the incidence of prostate cancer was reduced by more than 30% in the lycopene group. In addition, clinical trials have revealed the activity of lycopene in suppressing tumor growth and metastasis, which is particularly effective for pancreatic, lung and gastric cancers.

Due to its beneficial effects, lycopene is now recognized as an excellent health food supplement in the 21st century and is of interest worldwide. Since 1990, vast assets and efforts have been devoted to relevant research and the development of drugs, food supplements, foods and cosmetics containing lycopene, especially in developed countries including the United States, Western Europe, Japan and Israel. ing.

As the antioxidant, it is preferable to use tomato extract. The tomato extract preferably contains (but is not essential) natural compounds such as β-carotene, tocopherol, phytosterol, phospholipid, phytoene, and phytofluene in addition to lycopene. It is particularly preferable to contain polyphenols, because the interaction as an antioxidant can be expected. The content of lycopene (HPLC method) in the tomato extract is preferably 1% to 50%, more preferably 10% to 30%. This is because a high effect can be expected with a small amount of ingestion. In addition, the content of natural tomatoferol (as dry weight) in the tomato extract is 0.5% or more, the content of β-carotene is 0.1% or more, and the content of phytoene / phytofluene is 0.5% or more. Is more preferable.

The method for producing the tomato extract is not particularly limited, and the tomato extract can be produced using a known method. For example, it can be obtained by separating crushed tomatoes into a liquid component and a fiber component by a method such as centrifugation or standing, extracting the fiber component with various organic solvents such as ethyl acetate and ethanol, and drying the tomato. .. Since the fiber component is rich in components such as lycopene and polyphenols, the active ingredient can be effectively extracted. The tomatoes may be appropriately heat-treated or filtered before and after crushing.

As the polyphenol, known substances can be used regardless of whether they are synthetic or natural products, and for example, flavonoids, catalase, peroxidase, SOD (superoxide dismutase), ascorbic acid, tocopherol and the like are preferably used. It is also possible to use an extract extracted from various natural substances such as roots, stems, leaves, fruits, fruit skins and bark of plants as polyphenols.

The organic solvent used for the extraction of trapa plants, carotenoids, and polyphenols is not particularly limited, and one or more organic solvents may be used alone or in combination. Extraction may be performed only once, or may be performed twice or more using one kind or two or more kinds of organic solvents. As the organic solvent, for example, an aromatic organic solvent such as benzene, an aliphatic organic solvent such as hexane, and a polar solvent such as ketone, ether, alcohols, ethyl acetate, and dichloromethane are appropriately selected and used. It is preferred that the organic solvent is finally separated and only the dried extract is separated.

As the solvent used for hot water extraction, a mixed solvent (aqueous solvent) in which water, an aqueous solution, or any one or more solvents that are mixed with water and water are mixed at an arbitrary ratio can be used, but a preferable extraction solvent is used. Is an aqueous solvent in which water, methanol, ethanol and any two or more of these are mixed at an arbitrary ratio, and particularly preferable extraction solvents are water, ethanol and water which are organic solvents recognized as food additives. Is an aqueous solvent in which the above is mixed at an arbitrary ratio. The temperature of the extraction solvent may be any temperature above room temperature and below the boiling point of the extraction solvent, but it is preferably determined in consideration of extraction efficiency, heat resistance of the object to be extracted, volatility and the like.

When water or an aqueous solvent is used as the extraction solvent, an acid, a base, a salt or the like may be appropriately contained in order to improve the extraction efficiency. The temperature and pH of the water used for extraction are not particularly limited, but the pH is preferably near neutral, more specifically pH 4 to 9, and pH 6 to 8 in consideration of use in a living body. It is more preferable to have. If necessary, a heated extraction solvent may be used to improve the extraction efficiency.

Hot water extraction can be performed by any known method. Hereinafter, the Trapaceae plant will be described, but the same procedure is used when extracting an antioxidant extract from various natural products. A method of mixing one or both of the pericarp and fruit of a trapaceae plant in a solvent for a predetermined time and then separating them from solids by filtration, centrifugation, decantation, etc., or a continuous extraction method such as a Soxhlet extraction method should be used. Can be done.

Pretreatment by dialysis, ultrafiltration, filtration, column chromatography, etc. to remove high molecular weight components, insoluble matter, etc. before separating one or more compounds from the solvent extract of the skin of a trapaceae plant. May be done.

When removing insoluble matter or the like by filtration, an adsorbent such as activated carbon, bentonite, or celite or a filtration aid may be added, if necessary, in order to remove impurities. In particular, when it is used in the state of an extract, it is preferable to perform sterilization filtration with a membrane filter or the like.

The reactions targeted for inhibition of the anti-glycation / anti-oxidation / anti-carbonylation composition are the formation of a shift base by the reaction between the amino group of the protein and the reducing sugar, and the 1,2-enaminol or 2,3-endiol by the Amadori rearrangement. Any one or more reactions such as the production of Amadori rearrangement products and the production of polymerization products of the degradation products with amino acids, peptides or proteins.

By mixing the anti-glycation / antioxidant / anti-carbonylation composition with a carrier or the like, it can be used as a pharmaceutical composition having one or both of a therapeutic effect and a preventive effect on diabetes and related diseases and symptoms. Examples of the administration form of the pharmaceutical composition to humans or animals include oral, transrectal, parenteral (for example, intravenous administration, intramuscular administration, subcutaneous administration, etc.), and the dose is the pharmaceutical composition form. It is difficult to make an appropriate setting according to the administration method, purpose of use, age, body weight, and symptom of the subject to be administered, but in the case of humans, the active ingredient generally contained in the pharmaceutical product. The amount is preferably 0.1 to 2000 mg / day for an adult per day. Of course, since the dose varies depending on various conditions, a dose smaller than the above dose may be sufficient, or a dose exceeding the above range may be required.

When prepared as an oral preparation, it can be prepared in the form of tablets, granules, powders, capsules, coatings, liquids, suspensions, chewables, troches, etc., and when it is prepared as a parenteral preparation, it is an injection. , Drips, suppositories, etc. can be prepared. Any known method can be used for the formulation. For example, an advanced glycation end product production inhibitor can be blended with a pharmaceutically acceptable carrier or diluent, stabilizer, and other desired additives to obtain the above-mentioned desired dosage form.

Foods containing anti-glycation / anti-oxidation / anti-carbonylation compositions include foods containing anti-glycation / anti-oxidation / anti-carbonylation compositions, or are usually used for foods or health foods such as capsules and tablets. It can take any form. There are no particular restrictions on the types of foods that can be mixed, for example, liquid (fluid) foods such as coffee, fruit juice, refreshing drinking water, beer, milk, miso juice, soup, black tea, tea, nutritional supplements, syrup, margarine, and jam. It can also be added to main foods such as rice, bread, potato products, rice cakes, candy, chocolate, sprinkles, ham, sausages, candies, gums and other solid foods, side foods, confectionery and seasonings. Depending on the application, it may be formed into powder, granules, tablets or the like. Further, if necessary, it may be appropriately mixed with excipients, bulking agents, binders, thickeners, emulsifiers, coloring agents, flavorings, food additives, seasonings and the like.

In addition to foods for human consumption, when an anti-glycation / antioxidant / anti-carbonylation composition is mixed in a feed and administered to animals such as livestock and pets, it is previously added to the feed material. It can be mixed and prepared as a feed with added functionality. Further, the anti-glycation / antioxidant / anti-carbonylation composition can be added to the feed and administered. That is, foods containing an anti-glycation / anti-oxidation / anti-carbonylation composition as an active ingredient are added to livestock such as pigs, chickens, cows, horses and sheep, and feeds such as fish and pets (dogs, cats and birds). As a result, it is safe and can be used as a functional feed having one or both of a therapeutic effect and a preventive effect on diabetes and related diseases and symptoms.

Next, an embodiment will be described.

Twenty-six Japanese women aged 20 to 64 years were divided into two groups (Example 1 group and Comparative Example 1 group), and 1 sample of Example and 1 sample of Comparative Example were given once a day, respectively. It was orally ingested for 12 weeks. I explained the function to the two groups, but did not tell the group to which they belong. The composition of Example 1 sample and Comparative Example 1 sample is shown below.

Subjects are those who are concerned about skin sagging, wrinkles, dryness, etc., right and left cheeks, right chin, upper back, upper shin, right foot back every October-March (winter) We recruited people who had rough skin due to dryness, itching, or dusting.

As the Hishi extract, the peel of Tobishi was extracted with hot water, concentrated, dextrin was added as an excipient, sterilized by heating at 90 ° C. for 15 minutes, filtered, and then spray-dried. Trapa japonica extract component in the dry component is 67%, dextrin is 33%, polyphenol content in the whole is 25-40% or more (colorimetric method), gallic acid 4.13% or more, ellagic acid 1.15%. That was all.

As the antioxidant, tomato extract was used. The content of lycopene is 15% to 30% (HPLC method), the content of natural tocopherol is 1.5% or more, the content of phytoene / phytofluene is 1.0% or more, and the content of β-carotene is 0. It was more than 2%.

As the tomato extract, oleoresin produced by the following method was used. The tomatoes used as raw materials were crushed and heat-treated. The ground tomatoes were separated into supernatant and pulp. The pulp was subjected to solvent extraction (ethyl acetate) and the lycopene extract was separated from the solvent, thereby giving oleoresin containing lycopene. The solvent used was recovered.

Before ingestion, after 4 weeks of ingestion, 8 weeks after ingestion, and 12 weeks after ingestion, the subject's skin, blood, urine, height, weight, blood pressure, pulse, antioxidant power (BAP), oxidative stress level (d-ROM) ) And other items that indicate physical condition and health condition were inspected, visually observed and interviewed, and subjective symptoms were investigated (questionnaire).

The test meal intake period was 12 weeks. During the test period, he was instructed to keep a regular life, avoid radical exercise and overdrinking, and maintain the same quantity and quality as in daily life regarding diet and exercise.

As a method of ingestion, the test meal was ingested once a day with 1 capsule, sufficient water, or hot water before breakfast. If it could not be taken before breakfast, it should be taken during the day and not carried over to the next day.

1) Efficacy test Skin measurements were performed 4 times in total for the following items: at the start of ingestion, 4 weeks after ingestion, 8 weeks after ingestion, and 12 weeks after ingestion. In the measurement environment, the temperature and humidity were controlled at a temperature of 22 ± 2 ° C and a humidity of 50 ± RH 10%, and after washing the face, the patient was allowed to stand by for 20 minutes to acclimatize.

Three sites were set as the measurement sites for "skin water evaporation amount" at the following positions.
1. The intersection of the vertical line from the right corner of the right cheek and the horizontal line from the right nose was used as the measurement site, and a sticker was attached to the upper part.
2. The measurement site was 10 cm below the bottom of the plate on the right shin and right knee, and a sticker was attached to the top.
3. The back of the right foot A sticker was attached to the upper part of the measurement site, 4 cm from the base of the index finger of the right foot to the ankle side.

For the "skin water evaporation amount", TEWAMETER TM 300 (Courage + KhazakaElectronic Gmbh) was used, and 6 sites were continuously measured for 1 minute, and a stable 5 seconds was adopted. For a stable 5 seconds, the mean value and standard deviation were calculated for 5 consecutive seconds, and the mean value in the section with the smallest standard deviation was recorded as the amount of skin water evaporation.

"Skin color measurement" uses SPECTROPHOTOMETER (NF333 / Nippon Denshoku Industries Co., Ltd.), and color measurement by the Lab method: L * value (brightness), a * value (redness) and b * value (saturation) are on the right. The medial part of the forearm was measured 3 times and the average value was recorded as skin color.

"Skin condition photography" uses VISIA-Generation7 (Canfield Scientific), the right face is placed on the fixed base of the device, the subject is lightly closed his eyes, and the subject is automatically photographed with the device's imaging program in a resting state.・ The red part was analyzed.

"Percentile" is the ranking counted from the bottom compared to 100 people of the same age. It indicates how many people have worse numerical values for the measurement item than the subject, and the larger the numerical value, the better. The maximum is "99". A large value indicates that there are few stains. The "red part (Percentile)" is the ranking counted from the bottom compared to 100 people of the same age. It indicates how many people have worse numerical values for the measurement item than the subject, and the larger the numerical value, the better. The maximum is "99". A large value indicates that there are few stains. Larger values indicate more red areas. The "texture (score)" is a numerical value of the area of the analysis range and the color depth information. The higher the score, the more uneven the color on the skin surface, and the lower the score, the less the color unevenness on the skin surface. It is shown that.

The "questionnaire survey" asked the subjects to answer the following questions on the web regarding the recent skin condition.
1. 1. Moisturizing elbows, knees and heels 2. Moisturizing the neck The left end is "no moisturizing (the worst condition you can imagine)": 1 and the right end is "ideal moisturizing (the best condition you can imagine)": 100 I gave an answer based on the score.

In the questionnaire survey, the amount of change (difference) from the start of ingestion, 4 weeks after ingestion, 8 weeks after ingestion, and 12 weeks after ingestion was compared between the groups by the Mann-Whitney U test. The statistical analysis software is Dr. SPSS II for Windows (SPSS Co., Ltd.) was used, and the significance level was set to less than 5% in both tests in both tests.

The results are shown in Table 2.

The intake rate of the test meal was 100%, and there were no subjects who did not comply with the management items during the test period. On the other hand, this test has been conducted under the epidemic of new coronavirus infection (COVID-19) from the end of 2019 (hereinafter referred to as corona-ka), and most of the 17 people who dropped out due to personal reasons are self-restraint due to corona-ka. Because of this, we conducted a questionnaire to see if the subjects who participated until the final observation after the test felt some kind of anxiety or mental stress due to being forced to refrain from living in Korona-ka. The case study group discussed that the subjects who felt the stress of Korona-ka should be treated as the subjects excluded from the analysis, and the final number of subjects for the analysis was 26.

The ages of 13 subjects (male: 2 and female: 11) in the test diet group were 51.2 ± 12.3 years (male: 54.5 ± 3.5 years, female: 50.5 ± 13.4 years). The ages of 13 subjects (male: 2 and female: 11) in the placebo diet group were 51.0 ± 10.1 years (male: 59.0 ± 4.2 years, female: 49.5 ± 10.2 years).

In "Skin water evaporation: right cheek", the difference value, which is the difference between the value of each observation and the value before ingestion, was -2.54 ± 5.17 after 4 weeks of ingestion and -5.40 after 8 weeks of ingestion in the test meal group. It was ± 10.12 and -6.41 ± 6.79 12 weeks after ingestion. On the other hand, in the placebo diet group, it was 0.46 ± 4.83 after 4 weeks of ingestion, 0.24 ± 10.29 after 8 weeks of ingestion, and 3.00 ± 8.08 after 12 weeks of ingestion.
A significant difference was observed 12 weeks after ingestion (P = 0.004) in the comparison between the test diet group and the placebo diet group.

In "Skin water evaporation: right shin", the difference value, which is the difference between the value of each observation and the value before ingestion, is -1.36 ± 1.62 after 4 weeks of ingestion and -2.50 after 8 weeks of ingestion in the test meal group. It was ± 1.77 and -3.10 ± 1.93 12 weeks after ingestion. On the other hand, in the placebo diet group, it was -1.05 ± 2.60 after 4 weeks of ingestion, -1.19 ± 3.59 after 8 weeks of ingestion, and -0.90 ± 2.89 after 12 weeks of ingestion. A significant difference was observed 12 weeks after ingestion (P = 0.032) in the comparison between the test diet group and the placebo diet group.

In "Skin water evaporation: back of right foot", the difference value, which is the difference between the value of each observation and the value before ingestion, was -0.02 ± 3.20 after 4 weeks of ingestion and -2.54 after 8 weeks of ingestion in the test meal group. It was ± 3.64 and -1.99 ± 3.67 12 weeks after ingestion. On the other hand, in the placebo diet group, it was 0.20 ± 3.33 after 4 weeks of ingestion, 0.73 ± 2.64 after 8 weeks of ingestion, and -0.57 ± 3.03 after 12 weeks of ingestion. A significant difference was observed 8 weeks after ingestion (P = 0.015) in the comparison between the test diet group and the placebo diet group.

"Skin color measurement: L * value brightness"
The difference value, which is the difference between the value of each observation and the value before ingestion, was 0.214 ± 1.114 after 4 weeks of ingestion, 0.437 ± 1.002 after 8 weeks of ingestion, and 0.638 ± 0.883 after 12 weeks of ingestion in the test meal group. rice field. On the other hand, in the placebo diet group, it was -0.086 ± 0.958 after 4 weeks of ingestion, -0.515 ± 0.834 after 8 weeks of ingestion, and 0.086 ± 0.760 after 12 weeks of ingestion. A significant difference was observed 8 weeks after ingestion (P = 0.015) in the comparison between the test diet group and the placebo diet group. No significant difference was confirmed in the a * value (redness) and b * value (saturation).

In "Skin condition photography: Percentile", the difference value, which is the difference between the value of each observation and the value before ingestion, was -3.92 ± 18.92 after 4 weeks of ingestion and 2.23 after 8 weeks of ingestion in the test meal group. It was ± 14.28 and 3.69 ± 12.52 12 weeks after ingestion. On the other hand, in the placebo diet group, it was -5.54 ± 12.54 after 4 weeks of ingestion, -7.46 ± 15.03 after 8 weeks of ingestion, and -6.54 ± 12.49 after 12 weeks of ingestion. A significant difference was observed 12 weeks after ingestion (P = 0.048) in the comparison between the test diet group and the placebo diet group.

In "Skin condition photography: texture (score)), the difference value, which is the difference between the value of each observation and the value before ingestion, is 0.008 ± 1.332 at 4 weeks after ingestion and 0.008 ± 1.332 after 8 weeks of ingestion in the test meal group- It was 0.530 ± 1.709 and -0.177 ± 2.760 12 weeks after ingestion. On the other hand, in the placebo diet group, it was 1.305 ± 1.926 after 4 weeks of ingestion, 1.187 ± 2.143 after 8 weeks of ingestion, and 1.038 ± 2.178 after 12 weeks of ingestion. A significant difference was observed 8 weeks after ingestion (P = 0.033) in the comparison between the test diet group and the placebo diet group.

In "Skin condition photography: red part (Percentile)", the difference value, which is the difference between the value of each observation and the value before ingestion, is 7.85 ± 16.01 after 4 weeks of ingestion and 10.54 after 8 weeks of ingestion in the test meal group. It was ± 19.95 and 2.46 ± 21.46 12 weeks after ingestion. On the other hand, in the placebo diet group, it was -4.54 ± 7.86 after 4 weeks of ingestion, -1.69 ± 6.07 after 8 weeks of ingestion, and -3.92 ± 7.54 after 12 weeks of ingestion. A significant difference was observed 4 weeks after ingestion (P = 0.022) in the comparison between the test diet group and the placebo diet group.

To confirm efficacy, subjects' blood was taken and plasma pentosidine, an indicator marker for saccharified and carbonylated proteins, was measured. Blood was collected at the start of ingestion, 4 weeks after ingestion, 8 weeks after ingestion, and 12 weeks after ingestion. It was -0.0037 ± 0.00186 after 4 weeks of ingestion, -0.0073 ± 0.0169 after 8 weeks of ingestion, and -0.0163 ± 0.0112 after 12 weeks of ingestion, and the decrease in the values gradually increased. On the other hand, in the placebo diet group, it was 0.0198 ± 0.0229 after 4 weeks of ingestion, 0.0115 ± 0.0255 after 8 weeks of ingestion, and 0.0019 ± 0.0169 after 12 weeks of ingestion. A significant difference was observed between the test diet group and the placebo diet group at 4 weeks after ingestion (P = 0.008), 8 weeks after ingestion (P = 0.036), and 12 weeks after ingestion (P = 0.004).

To confirm safety, "weight, BMI, blood pressure, pulse", "blood test (leukocyte count, red blood cell count, blood pigment level, hematocrit, hematocrit," at the start of ingestion, 4 weeks after ingestion, 8 weeks after ingestion, and 12 weeks after ingestion. MCV, MCH, MCHC, red blood cell count) and blood biochemical tests (total cholesterol, LDL cholesterol, HDL cholesterol, neutral fat, total protein, albumin, urea nitrogen, creatinine, uric acid, AST, ALT, γ-GT, lactic acid Dehydrogenase, creatinine kinase, blood glucose, HbA1c, oxidized LDL) and urine test (protein, sugar, urobilinogen, bilirubin, specific gravity, pH, ketone body, occult blood) ”were performed, but there was no significant difference in any of them, and the standard values. The safety was confirmed without large numerical fluctuations that would exceed the range of.

In addition, we conducted daily questionnaire responses regarding changes in the physical condition of the subjects and confirmed the changes in the physical condition of the subjects. No significant event was confirmed, and safety was confirmed.

<Operation and effect>
According to the above configuration, the composition of the present invention is:
It was made to contain a processed product of Trapa japonica and an antioxidant capable of removing active oxygen.

As a result, in addition to the anti-glycation and anti-carbonylation effects of Trapa plants, active oxygen that causes the reaction can be removed, thus suppressing saccharification, carbonylation, and oxidation, which are the three major factors of aging. However, it can exhibit a high anti-aging effect.

In the composition, the processed product of the genus Trapa is a processed product of the genus Trapa selected from the group consisting of trapa natans, trapa natans, trapa incisa, trapa incisa, trapa japonica, and trapa natans.

As a result, saccharification, carbonylation, and oxidation can be effectively suppressed by the Maillard inhibitory effect of Trapa japonica, which is characteristic of Trapa japonica plants.

In the composition
The processed product of the genus Trapa is a crushed product and / or an extract of the peel of the genus Trapa.

As a result, the trapa japonica can be efficiently ingested from the pericarp rich in trapa japonica, which is characteristic of trapa japonica plants.

In the composition, the active oxygen is
Free radicals such as superoxide anion (O2), hydroxy radical (・ OH), nitrogen monoxide radical (NO ・), hypochlorite ion (ClO), hydrogen peroxide (H2O2), singlet oxygen (1O2) It is characterized by being a low molecular weight oxygen compound in a state of possession or a state in which free radicals can be easily generated.

As a result, active oxygen present throughout the whole body can be reduced, and saccharification, carbonylation, and oxidation reactions can be suppressed.

In the composition, the antioxidant is characterized by being a carotenoid.

As a result, since carotenoids having high antioxidant capacity can be used as the antioxidant, the causative substance can be reduced, and the reactions of saccharification, carbonylation, and oxidation reaction can be effectively suppressed.

In the composition
The antioxidant is characterized by being a tomato extract.

This makes it possible to obtain a naturally-derived composition having a high anti-aging effect, which is composed of a trapa japonica plant and a tomato extract.

In the composition, the antioxidant is
It is characterized by containing carotenoids and polyphenols.

As a result, it is possible to provide a composition that is effective for more people by reducing individual differences due to the effects of various types of antioxidants.

In the composition, the antioxidant is
It is characterized by being a polyphenol.

As a result, a composition having a high anti-aging effect can be obtained by the combined action of many kinds of polyphenols.

In the composition
The composition is characterized by being a food or drink, a medicinal product, or a supplement.

Thereby, the composition having an anti-aging effect can be ingested in an appropriate form according to an individual's condition and symptom.

The composition is characterized by being a soft capsule, a hard capsule, a liquid preparation, a jelly, a gummies, a tablet, a granule, a powdered beverage, or a powder.

Thereby, the composition having an anti-aging effect can be ingested in an appropriate form according to an individual's taste, physical condition and the like.

The present invention can be applied to supplements and the like that are effective in preventing aging and improving skin quality.

Claims (9)

A composition characterized by containing a processed product of the genus Trapa and an extract of tomato extract. The tomato extract is
The composition according to claim 1, wherein the composition contains 1 to 50% of lycopene. The tomato extract is
The composition according to claim 2, wherein the composition contains polyphenol as a component other than lycopene. The tomato extract is
The composition according to claim 1, wherein the composition is extracted with ethyl acetate. The first aspect of claim 1, wherein the processed product of the genus Trapa is a processed product of the genus Trapa selected from the group consisting of trapa natans, trapa natans, trapa incisa, trapa incisa, trapa japonica, and trapa natans. Composition. The composition according to claim 1 or 5, wherein the processed product of the genus Trapa is a pulverized product and / or an extract of the peel of the genus Trapa. The composition according to any one of claims 1 to 6, which is for food and drink or for pharmaceutical purposes. The composition according to any one of claims 1 to 6, wherein the composition is for supplements. The composition according to any one of claims 1 to 8, wherein the composition is a soft capsule, a hard capsule, a liquid preparation, a jelly, a gummy candy, a tablet, a granule, a powdered beverage, or a powder.

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