JP2022066911A - Production method of rice - Google Patents
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- JP2022066911A JP2022066911A JP2020175518A JP2020175518A JP2022066911A JP 2022066911 A JP2022066911 A JP 2022066911A JP 2020175518 A JP2020175518 A JP 2020175518A JP 2020175518 A JP2020175518 A JP 2020175518A JP 2022066911 A JP2022066911 A JP 2022066911A
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- fumaric acid
- bacillus subtilis
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- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 125
- 235000009566 rice Nutrition 0.000 title claims abstract description 124
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 17
- 240000007594 Oryza sativa Species 0.000 title description 117
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 claims abstract description 62
- 239000001530 fumaric acid Substances 0.000 claims abstract description 31
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- 238000010411 cooking Methods 0.000 claims abstract description 20
- -1 glycerin fatty acid ester Chemical class 0.000 claims abstract description 17
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- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- RGHNJXZEOKUKBD-UHFFFAOYSA-N D-gluconic acid Natural products OCC(O)C(O)C(O)C(O)C(O)=O RGHNJXZEOKUKBD-UHFFFAOYSA-N 0.000 description 1
- 239000004278 EU approved seasoning Substances 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 1
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- ZVQOOHYFBIDMTQ-UHFFFAOYSA-N [methyl(oxido){1-[6-(trifluoromethyl)pyridin-3-yl]ethyl}-lambda(6)-sulfanylidene]cyanamide Chemical compound N#CN=S(C)(=O)C(C)C1=CC=C(C(F)(F)F)N=C1 ZVQOOHYFBIDMTQ-UHFFFAOYSA-N 0.000 description 1
- 235000019631 acid taste sensations Nutrition 0.000 description 1
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- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 description 1
- 230000002421 anti-septic effect Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 235000021329 brown rice Nutrition 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
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- 239000005417 food ingredient Substances 0.000 description 1
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- 239000000174 gluconic acid Substances 0.000 description 1
- 235000012208 gluconic acid Nutrition 0.000 description 1
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- 239000003779 heat-resistant material Substances 0.000 description 1
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- 239000001632 sodium acetate Substances 0.000 description 1
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- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
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- Cereal-Derived Products (AREA)
Abstract
Description
本発明は、米飯の製造法に関し、さらに詳しくは、酸性臭のような刺激臭の発生や酸味もなく、米由来の枯草菌に対しても優れた静菌効果を有する米飯の製造法に関する。 The present invention relates to a method for producing cooked rice, and more particularly to a method for producing cooked rice having an excellent bacteriostatic effect against Bacillus subtilis derived from rice without generating a pungent odor such as an acidic odor or having an acid taste.
従来より、白米、おにぎり、おこわ等の米飯食品においては、衛生管理をより徹底する観点から、保存・販売時における菌の増殖を抑えるために、酢酸やグルコン酸等の酸味料やpH調整剤を添加する技術などが数多く知られている。 Conventionally, in cooked rice foods such as white rice, rice balls, and rice balls, acidulants such as acetic acid and gluconic acid and pH adjusters have been used to suppress the growth of bacteria during storage and sales from the viewpoint of thorough hygiene management. Many techniques for adding are known.
例えば、1)水洗白米を0.2~1.0%の酢酸溶液に浸漬後、加熱炊飯して酢酸を米粒の中心部にまで浸透せしめることを特徴とする炊飯米の保護法(例えば、特許文献1参照)、2)米に水を加えて加熱し米飯を製造する場合、酢酸、酪酸その他脂肪酸を除く脂肪族の有機酸(この有機酸を含む)を加えてpHを6.5以下として加熱することを特徴とする米飯の製造法(例えば、特許文献2参照)、3)炊飯用の米を酢酸溶液に浸漬して内部まで酢酸を浸透させて処理し、この酢酸処理後の米をクエン酸ナトリウムまたは酢酸ナトリウムのような食品添加物である有機酸塩を含む溶液で中和し、酢酸の香り、味を除去することにより、炊飯用の米を品質改良することを特徴とする炊飯用米の品質管理方法(例えば、特許文献3参照)などが知られている。 For example, 1) A method for protecting cooked rice (for example, a patent), which comprises immersing washed white rice in a 0.2 to 1.0% acetic acid solution and then heating the rice to allow the acetic acid to permeate into the center of the rice grain. (Refer to Reference 1), 2) When cooked rice is produced by adding water to rice, add an aliphatic organic acid (including this organic acid) excluding acetic acid, butyric acid and other fatty acids to set the pH to 6.5 or less. A method for producing cooked rice characterized by heating (see, for example, Patent Document 2), 3) Rice for cooking rice is dipped in an acetic acid solution to allow acetic acid to permeate the inside, and the rice after the acetic acid treatment is treated. Rice cooking is characterized by improving the quality of rice for cooking by neutralizing it with a solution containing an organic acid salt which is a food additive such as sodium citrate or sodium acetate to remove the aroma and taste of acetic acid. A quality control method for cooked rice (see, for example, Patent Document 3) is known.
上記特許文献1~2において、用いる酢酸は、防腐力の点から優れているものであるが、揮発酸であるため、炊飯米に独特の刺激臭を付与してしまう傾向や酸味を付与してしまう場合があり、特に、この種の炊飯米を電子レンジ等で再加熱などすると、酢酸臭がより強く発生して風味を低下させるという欠点があった。上記特許文献3の中和処理するものでは、酢酸臭の発生を抑えるものとなるが、処理法が複雑化するなどの課題があり、改善などが切望されているのが現状である。 The acetic acid used in the above Patent Documents 1 and 2 is excellent in terms of antiseptic power, but since it is a volatile acid, it imparts a tendency and acidity to give a peculiar pungent odor to cooked rice. In particular, when this type of cooked rice is reheated in a microwave oven or the like, there is a drawback that an acetic acid odor is generated more strongly and the flavor is deteriorated. The neutralization treatment of Patent Document 3 suppresses the generation of acetic acid odor, but there is a problem that the treatment method becomes complicated, and improvement is eagerly desired at present.
一方、枯草菌は、土中に存在する菌で、何処にでもいるものである。特に、イネ藁には普通に存在し、バチルス属に含まれる腐敗菌の1種である。この枯草菌は芽胞(がほう)と呼ばれる外皮を形成し、これが熱に強いため、炊飯時の熱(100~120℃)では生き残ってしまい、炊飯後には活動を開始し、変色や匂いを出したりする。しかも、厄介なことに精白米の段階では、これらの菌に汚染されているかどうかを確認することが目視ではできないものである。上記枯草菌は腐敗菌であるため、人体にはそれほど害は少ないものであるが、フードサービスではあれだけ変色や匂いが出てしまうと食べてくれる人は少なく大変な損害を生じかねないものとなる。 Bacillus subtilis, on the other hand, is a bacterium that exists in the soil and is ubiquitous. In particular, it is a type of putrefactive bacterium that is normally present in rice straw and is contained in the genus Bacillus. This Bacillus subtilis forms an outer skin called spores, which are resistant to heat, so they survive the heat of cooking rice (100-120 ° C), and after cooking, they start their activity and give off discoloration and smell. Or something. Moreover, troublesomely, at the stage of polished rice, it is not possible to visually confirm whether or not the rice is contaminated with these bacteria. Since the above-mentioned Bacillus subtilis is a putrefactive bacterium, it is not so harmful to the human body, but in the food service, if such discoloration or odor comes out, few people will eat it and it may cause great damage. Become.
本発明は、上記従来技術の課題及び現状などに鑑み、これを解消しようとするものであり、取り扱い性に優れ、酸味を感じることなく、かつ、酸性臭のような刺激臭の発生もなく無臭であり、安全かつ効率よく、米由来の枯草菌に対しても優れた静菌効果を有する米飯の製造法を提供することを目的とする。 The present invention has been made in view of the above-mentioned problems of the prior art and the current state of affairs, and is intended to solve the problems. It is an object of the present invention to provide a method for producing cooked rice, which is safe and efficient and has an excellent bacteriostatic effect on Bacillus subtilis derived from rice.
本発明者らは、上記従来の課題等について、鋭意検討した結果、食品の酸味料として使用されている有機酸の中で、フマル酸を米由来の枯草菌に対して利用できないかを検討・実証試験などを施したところ、上記目的の米飯の製造法が得られることを見出し、本発明を完成するに至ったのである。
すなわち、本発明は、米飯の炊飯の際に、炊飯物全量に対して、フマル酸を0.03~0.2質量%添加して炊飯することを特徴とする米飯の製造方法である。
好ましくは、上記フマル酸と共に、グリセリン脂肪酸エステル、ポリグリセリン脂肪酸エステル、キサンタンガムから選ばれる少なくとも1種を添加することが望ましい。
As a result of diligent studies on the above-mentioned conventional problems, the present inventors examined whether fumaric acid can be used against rice-derived bacillus among the organic acids used as acidulants in foods. As a result of conducting verification tests and the like, it was found that the above-mentioned method for producing rice and rice could be obtained, and the present invention was completed.
That is, the present invention is a method for producing cooked rice, which comprises adding 0.03 to 0.2% by mass of fumaric acid to the total amount of cooked rice when cooking cooked rice.
It is preferable to add at least one selected from glycerin fatty acid ester, polyglycerin fatty acid ester, and xanthan gum together with the fumaric acid.
本発明によれば、取り扱い性に優れ、酸味を感じることなく、かつ、酸性臭のような刺激臭の発生もなく無臭であり、安全かつ効率よく、米由来の枯草菌に対しても優れた静菌効果を有する米飯の製造法が提供される。
本発明の目的及び効果は、特に請求項において指摘される構成要素及び組み合わせを用いることによって認識され且つ得られるものである。本明細書における前述の一般的な説明及び後述の詳細な説明の両方は、例示的及び説明的なものであり、特許請求の範囲に記載されている本発明を制限するものではない。
According to the present invention, it is easy to handle, does not feel acidity, is odorless without generating a pungent odor such as an acidic odor, is safe and efficient, and is excellent against Bacillus subtilis derived from rice. A method for producing cooked rice having a bacteriostatic effect is provided.
The object and effect of the present invention are recognized and obtained particularly by using the components and combinations pointed out in the claims. Both the general description described above and the detailed description below are illustrative and descriptive herein and are not intended to limit the invention described in the claims.
以下に、本発明の実施形態を詳しく説明する。但し、本発明の技術的範囲は、下記で詳述するそれぞれの実施の形態に限定されず、特許請求の範囲に記載された発明とその均等物に及ぶ点に留意されたい。
本発明の米飯の製造方法は、米飯の炊飯の際に、炊飯物全量に対して、フマル酸を0.03~0.2質量%添加して炊飯することを特徴とするものである。
Hereinafter, embodiments of the present invention will be described in detail. However, it should be noted that the technical scope of the present invention is not limited to the respective embodiments detailed below, but extends to the inventions described in the claims and their equivalents.
The method for producing cooked rice of the present invention is characterized in that when cooked rice is cooked, 0.03 to 0.2% by mass of fumaric acid is added to the total amount of cooked rice to cook the cooked rice.
用いるフマル酸は、米飯の製造の際に、米由来の枯草菌に対する静菌成分として使用するものであり、構造式〔HOOC-CH=CH-COOH(トランス体)〕で表され、水難溶性の結晶性の粉末である。
用いるフマル酸は、特に限定されないが、好ましくは、体積平均粒子径30μm以下、より好ましくは15μm以下、特に好ましくは10μm以下であり、原料として市販されているフマル酸を乾式又は湿式の方法で粉砕して上記好ましい粒径のものを使用する。また、原料製造時に好ましい粒径になるよう結晶化させて取り出したものを使用しても良い。
The fumaric acid used is used as a bacteriostatic component against Bacillus subtilis derived from rice in the production of cooked rice, is represented by the structural formula [HOOC-CH = CH-COOH (trans form)], and is sparingly soluble in water. It is a crystalline powder.
The fumaric acid used is not particularly limited, but is preferably a volume average particle diameter of 30 μm or less, more preferably 15 μm or less, and particularly preferably 10 μm or less, and the fumaric acid commercially available as a raw material is pulverized by a dry or wet method. Then, the one having the above-mentioned preferable particle size is used. Further, it may be used that has been crystallized and taken out so as to have a preferable particle size at the time of raw material production.
用いるフマル酸の含有量は、炊飯物全量に対して、0.03~0.2質量%(以下において、「質量%」を単に「%」として表記する)、更に好ましくは、0.03~0.1%とすることが望ましい。
このフマル酸の含有量が0.03%未満であると、本発明の効果が発揮できないことがあり、一方、0.2%超過であると、酸味を呈し、好ましくない。
なお、本発明において、「炊飯物全量」とは、炊飯する際に用いる場合のお米、水、お米以外の食品原料、添加するフマル酸、その他の添加成分を含めた全体量(100%)を意味する。
The content of fumaric acid used is 0.03 to 0.2% by mass (hereinafter, "mass%" is simply referred to as "%"), more preferably 0.03 to 0.2% by mass, based on the total amount of cooked rice. It is desirable to set it to 0.1%.
If the content of fumaric acid is less than 0.03%, the effect of the present invention may not be exhibited, while if it exceeds 0.2%, a sour taste is exhibited, which is not preferable.
In the present invention, the "total amount of cooked rice" is the total amount (100%) including rice, water, food ingredients other than rice, fumaric acid to be added, and other additive components when used for cooking rice. ) Means.
また、本発明方法において、好ましくは、フマル酸の溶解性向上の点、フマル酸の分散性向上の点から、上記所定量のフマル酸と共に、グリセリン脂肪酸エステル、ポリグリセリン脂肪酸エステル、キサンタンガムから選ばれる少なくとも1種を添加することが好ましい。
これらの成分は食品添加物として使用されているものであり、その安全性は確認されている。
Further, in the method of the present invention, it is preferably selected from glycerin fatty acid ester, polyglycerin fatty acid ester, and xanthan gum together with the above-mentioned predetermined amount of fumaric acid from the viewpoint of improving the solubility of fumaric acid and improving the dispersibility of fumaric acid. It is preferable to add at least one kind.
These ingredients are used as food additives, and their safety has been confirmed.
上記グリセリン脂肪酸エステル、ポリグリセリン脂肪酸エステル、キサンタンガムは、フマル酸の難溶性(水への溶解度:25℃、0.63g/100mL)を勘案し、好ましくは、炊飯物全量に対して、合計含有量を好ましくは、0.0001~0.05%、更に好ましくは、0.0005~0.01%とすることが望ましい。 The above-mentioned glycerin fatty acid ester, polyglycerin fatty acid ester, and xanthan gum are preferably the total content of fumaric acid in consideration of the poor solubility in water (solubility in water: 25 ° C., 0.63 g / 100 mL) with respect to the total amount of cooked rice. Is preferably 0.0001 to 0.05%, and more preferably 0.0005 to 0.01%.
より好ましくは、フマル酸は、予め所定の体積平均粒子径となるフマル酸がグリセリン脂肪酸エステル、ポリグリセリン脂肪酸エステル、キサンタンガムで分散された水分散液を用いることが好ましく、特に好ましくは、炊飯物全量に対して、体積平均粒子径が10μm以下の上記所定量のフマル酸と、キサンタンガム0.0002~0.002%、グリセリン脂肪酸エステル0.001~0.005%、炊飯の際に用いる水とを合計したフマル酸水分散液を用いることが望ましい。 More preferably, as fumaric acid, it is preferable to use an aqueous dispersion in which fumaric acid having a predetermined volume average particle size is dispersed in glycerin fatty acid ester, polyglycerin fatty acid ester, and xanthan gum in advance, and particularly preferably, the total amount of cooked rice is used. On the other hand, the above-mentioned predetermined amount of fumaric acid having a volume average particle diameter of 10 μm or less, xanthan gum 0.0002 to 0.002%, glycerin fatty acid ester 0.001 to 0.005%, and water used for cooking rice were used. It is desirable to use the totaled aqueous dispersion of fumaric acid.
本発明方法において、炊飯に用いる原料となるお米は、通常の米飯食品に用いられるものであれば特に制限はなく、粳米、糯米、精白米、玄米、ジャポニカ米、インディカ米、餅米等その精白度合いや品種等に関係なく用いることができ、複数種の米を混合して用いてもよい。
また、本発明方法では、米飯の炊飯の際に、炊飯物全量に対して、フマル酸を0.02~0.2%添加して炊飯するものであれば、炊飯方法(炊飯温度、時間、炊飯機械、炊飯手段、炊飯工程等)は、特に制限はなく、例えば、家庭用や業務用の電気・ガスなどの加熱手段による所定の炊飯器、業務用炊飯機械などを用いて炊飯することができる。
また、白米以外の炊き込みご飯、釜飯、おこわ、赤飯等を製造する場合には米飯食品原料に米以外の原料を配合してもよく、また、おにぎり等の樹脂製の包装フィルムに包装する場合には、離型性の点から炊飯の際に食用油脂を添加してもよいものである。すなわち、本発明において、炊飯の際に用いる炊飯物の原料には、米の他に、必要に応じて、上記米以外の、調味料、糖質(トレハロース、異性化糖、ブドウ糖、乳糖、果糖等)、食用油脂、香辛料、出汁等を用いてもよいものである。
更に、本発明方法では、製造された炊飯米を容器や包装材に収容または包装する工程を含んでいてもよい。このような容器や包装材はマイクロ波加熱が可能な耐熱性材料から構成されていてもよい。すなわち、容器または包装材により収容または包装された米飯食品も、本発明の製造方法となる炊飯方法(炊飯物の製造法)の生産物の範囲内である。
In the method of the present invention, the rice used as a raw material for cooking rice is not particularly limited as long as it is used for ordinary rice foods, such as glutinous rice, glutinous rice, polished rice, brown rice, japonica rice, indica rice, and glutinous rice. It can be used regardless of the degree of whitening, the variety, etc., and a plurality of types of rice may be mixed and used.
Further, in the method of the present invention, when cooking cooked rice, if the cooked rice is cooked by adding 0.02 to 0.2% of fumaric acid to the total amount of cooked rice, the cooked rice method (rice cooking temperature, time, The rice cooking machine, rice cooking means, rice cooking process, etc.) are not particularly limited. can.
In addition, when producing cooked rice other than white rice, kamameshi, rice balls, red rice, etc., a raw material other than rice may be mixed with the cooked rice food raw material, or when packaging in a resin packaging film such as rice balls. In terms of releasability, edible oils and fats may be added when cooking rice. That is, in the present invention, in addition to rice, seasonings and sugars (trehalose, high fructose corn syrup, glucose, lactose, fructose) other than the above rice are used as raw materials for cooked rice. Etc.), edible oils and fats, spices, soup stock, etc. may be used.
Further, the method of the present invention may include a step of accommodating or packaging the produced cooked rice in a container or a packaging material. Such a container or packaging material may be made of a heat-resistant material capable of microwave heating. That is, the cooked rice food contained or packaged in the container or the packaging material is also within the range of the product of the rice cooking method (the method for producing the cooked rice) which is the production method of the present invention.
本発明方法では、米飯の炊飯の際に、炊飯物全量に対して、フマル酸を0.03~0.2%添加して炊飯するものであり、更に好ましくは、上記炊飯方法により炊飯された、炊飯米のpHは5.0以上、特に好ましくは、5~6.5となるように炊飯することが望ましい。
ここで炊飯米のpHが5.0以上とすることにより、酸味や酸臭を感じない米飯となり、一方、炊飯米のpHが6.5以下とすることにより、枯草菌等の雑菌の増殖を抑制することができる。
上記炊飯米のpH範囲(5~6.5)の調整は、用いるフマル酸、酢酸の各含有量、更に、フマル酸塩・クエン酸塩・リンゴ酸塩・乳酸塩等の有機酸塩を添加することで、調整することができる。
In the method of the present invention, when cooking cooked rice, 0.03 to 0.2% of fumaric acid is added to the total amount of cooked rice to cook the cooked rice, and more preferably, the cooked rice is cooked by the above-mentioned cooked rice method. It is desirable to cook rice so that the pH of the cooked rice is 5.0 or more, particularly preferably 5 to 6.5.
Here, when the pH of the cooked rice is 5.0 or more, the cooked rice does not feel acidity or acid odor, while when the pH of the cooked rice is 6.5 or less, the growth of germs such as Bacillus subtilis grows. It can be suppressed.
To adjust the pH range (5 to 6.5) of the cooked rice, the contents of fumaric acid and acetic acid to be used, and organic acid salts such as fumaric acid, citrate, malic acid, and lactate are added. By doing so, it can be adjusted.
以上のよう、本発明方法で得られる炊飯米は、取り扱い性に優れ、酸味を感じることなく、かつ、酸性臭のような刺激臭の発生もなく無臭であり、安全かつ効率よく、米由来の枯草菌に対しても優れた静菌効果を有するものである。
このようにして炊飯された炊飯米は、適宜、必要に応じて、容器詰めされ、低温流通などを経て使用に供され、必要に応じて電子レンジ等での再加熱等を行って食されることになる。
As described above, the cooked rice obtained by the method of the present invention is excellent in handleability, does not feel acidity, is odorless without generating a pungent odor such as an acidic odor, is safe and efficient, and is derived from rice. It also has an excellent bacteriostatic effect on Bacillus subtilis.
The cooked rice cooked in this way is appropriately packed in a container, distributed at a low temperature, etc., and then reheated in a microwave oven or the like to be eaten. It will be.
次に、下記試験例1~3により本発明を更に詳細に説明する。
〔試験例1〕
米(令和元年度米、品種:夢つくし米)150gに、水170ml及び下記組成の試験液サンプル1を0.75g添加し、炊飯器(NL-B805型(0.54Lサイズ)、象印マホービン社製)を用いて炊飯した。
炊飯した米10gを分取し、枯草菌芽胞(芽胞形成枯草菌:B.subtilis NBRC3134)が300CFU/0.1mlとなるように調整したものを添加し、25℃にて72時間保存したのち、枯草菌数を混釈法により測定した。コントロールは、水(精製水)100%である。
これらの結果を下記表1に示す。
Next, the present invention will be described in more detail with reference to Test Examples 1 to 3 below.
[Test Example 1]
Add 170 ml of water and 0.75 g of test solution sample 1 having the following composition to 150 g of rice (rice of the first year of Reiwa, variety: Yume Tsukushi rice), rice cooker (NL-B805 type (0.54 L size), Zojirushi Mahobin) Made) was used to cook rice.
10 g of cooked rice is taken, and Bacillus subtilis spores (spore-forming Bacillus subtilis: B.subtilis NBRC3134) adjusted to 300 CFU / 0.1 ml are added and stored at 25 ° C for 72 hours, and then Bacillus subtilis. The number of bacteria was measured by the pour method. The control is 100% water (purified water).
These results are shown in Table 1 below.
試験液サンプル1:
フマル酸(体積平均粒子径が10μm、以下同様) 30.0%
キサンタンガム 0.2%
グリセリン脂肪酸エステル 0.5%
水(精製水) 69.3%
Test solution sample 1:
Fumaric acid (volume average particle size is 10 μm, the same applies below) 30.0%
Xanthan gum 0.2%
Glycerin fatty acid ester 0.5%
Water (purified water) 69.3%
上記表1の結果から明らかなように、フマル酸0.07%を含有する試験液サンプル1では、芽胞を形成する枯草菌について経時的にも菌数の増加が見られず、優れた静菌効果を備えることを確認した。以下の試験例2~3において、該フマル酸の各濃度による枯草菌の静菌効果、並びに官能評価(酸味、臭い)などについて試験評価した。なお、上記試験例1で用いた枯草菌芽胞と、後述する試験例2以下で用いる枯草菌液とは、耐熱性を有する芽胞状態で添加しているか否かの点で相違するものである。 As is clear from the results in Table 1 above, in the test solution sample 1 containing 0.07% fumaric acid, no increase in the number of Bacillus subtilis forming spores was observed over time, and the bacillus was excellent. It was confirmed that it had an effect. In the following Test Examples 2 to 3, the bacteriostatic effect of Bacillus subtilis by each concentration of fumaric acid and the sensory evaluation (sour taste, odor) were tested and evaluated. The Bacillus subtilis spores used in Test Example 1 and the Bacillus subtilis solution used in Test Example 2 and below, which will be described later, differ in whether or not they are added in a heat-resistant spore state.
〔試験例2:実施例1及び比較例1~4〕
米(令和元年度米、品種:夢つくし米)150gに、水170ml及び下記表1に示す各濃度の有機酸(フマル酸、酢酸、クエン酸、リンゴ酸、乳酸)を添加し、炊飯器(NL-B805型(0.54Lサイズ)、象印マホービン社製)を用いて炊飯した。
炊飯した米10gを分取し、10~90CFU/0.1mlとなるように調整した下記菌種の枯草菌液を添加し、25℃にて72時間保存したのち、菌数を測定し、日持ち向上効果を評価した。
25℃、72時間保存後の菌数が、10の5乗台(105)以下を合格(評価:○)、105超過を(評価:×)とした。
また、味(酸味)については、パネラー5名により、飲食してもらい、官能評価(酸味又は刺激臭を感じる場合:×、酸味及び刺激臭を感じない場合:○)した。
更に、得られた炊飯米について、pHメーター:LAQUAact(HORIBA社製)を用いて、品温25℃で測定した。
なお、米に由来する枯草菌は、B.subtilis NBRC3134を評価菌種とした。
これらの結果を下記表2に示す。
[Test Example 2: Example 1 and Comparative Examples 1 to 4]
Rice cooker by adding 170 ml of water and organic acids (fumaric acid, acetic acid, citric acid, apple acid, lactic acid) of each concentration shown in Table 1 below to 150 g of rice (rice of the first year of Reiwa, variety: Yume Tsukushi rice). (NL-B805 type (0.54L size), manufactured by Zojirushi Mahobin Co., Ltd.) was used to cook rice.
Divide 10 g of cooked rice, add Bacillus subtilis solution of the following bacterial species adjusted to 10-90 CFU / 0.1 ml, store at 25 ° C for 72 hours, measure the number of bacteria, and improve shelf life. The effect was evaluated.
When the number of bacteria after storage at 25 ° C. for 72 hours was 10 to the 5th power (105) or less, it was accepted (evaluation: ◯), and when it exceeded 105, it was evaluated as (evaluation: ×).
Regarding the taste (sour taste), five panelists were asked to eat and drink, and the sensory evaluation (when the sour taste or the pungent odor was felt: ×, when the sour taste and the pungent odor were not felt: ○) was performed.
Further, the obtained cooked rice was measured at a product temperature of 25 ° C. using a pH meter: LAQUAact (manufactured by HORIBA).
As the Bacillus subtilis derived from rice, B. subtilis NBRC3134 was used as the evaluation strain.
These results are shown in Table 2 below.
無添加:pH6.86
Additive-free: pH 6.86
〔試験例3:実施例2~4及び比較例1~4〕
上記試験例1と同じ米150gに、水170ml及び下記表2に示す各濃度のフマル酸を添加し、上記試験例1と同じ炊飯器を用いて炊飯した。
炊飯した米10gを分取し、10~90CFU/0.1mlとなるように調整した上記試験例1と同様の枯草菌液を添加し、25℃にて96時間保存したのち、菌数を測定し、日持ち向上効果を上記試験例1と同様にして評価した。
また、味(酸味)、pHについては、上記試験例1と同様にして評価した。
これらの結果を下記表3に示す。
[Test Example 3: Examples 2 to 4 and Comparative Examples 1 to 4]
170 ml of water and fumaric acid having each concentration shown in Table 2 below were added to 150 g of the same rice as in Test Example 1 and cooked using the same rice cooker as in Test Example 1.
10 g of cooked rice was separated, the same Bacillus subtilis solution as in Test Example 1 above adjusted to 10 to 90 CFU / 0.1 ml was added, and the mixture was stored at 25 ° C. for 96 hours, and then the number of bacteria was measured. The shelf life improvement effect was evaluated in the same manner as in Test Example 1 above.
The taste (sour taste) and pH were evaluated in the same manner as in Test Example 1 above.
These results are shown in Table 3 below.
上記表1~3の結果から明らかなように、最適な、フマル酸の配合量は、0.03~0.20%であることが確認等でき、また、0.03%未満の濃度であると、日持ち効果を発揮できず、0.23%以上になると酸味を感じた。
これらにより、本発明範囲となる所定濃度のフマル酸を用いることにより、取り扱い性に優れ、酸味もなく、酸性臭のような刺激臭の発生もなく無臭であり、安全かつ効率よく、米由来の枯草菌に対しても優れた静菌効果を有する炊飯米が得られることがわかった。
As is clear from the results of Tables 1 to 3 above, it can be confirmed that the optimum blending amount of fumaric acid is 0.03 to 0.20%, and the concentration is less than 0.03%. The shelf life effect could not be exhibited, and when it became 0.23% or more, the acidity was felt.
As a result, by using fumaric acid at a predetermined concentration within the scope of the present invention, it is easy to handle, has no acidity, is odorless without the generation of pungent odors such as acidic odors, is safe and efficient, and is derived from rice. It was found that cooked rice having an excellent bacteriostatic effect on Bacillus subtilis can be obtained.
取り扱い性に優れ、酸味を感じることなく、かつ、酸性臭のような刺激臭の発生もなく無臭であり、安全かつ効率よく、米由来の枯草菌に対しても優れた静菌効果を有する米飯の製造法が提供される。 Cooked rice that is easy to handle, does not feel acidity, is odorless without the generation of pungent odors such as acidic odors, is safe and efficient, and has an excellent bacteriostatic effect against Bacillus subtilis derived from rice. Manufacturing method is provided.
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