JP2022044031A - Mosquito attractant, poison bait for mosquitoes and mosquito attracting method - Google Patents

Abstract

To provide a mosquito attractant that can satisfactorily attract mosquitoes.SOLUTION: A mosquito attractant contains at least one selected from the group consisting of seafood-derived components, seaweed-derived components and compounds contained therein.SELECTED DRAWING: None

Description

The present invention relates to a mosquito attractant, a mosquito poison bait and a method for attracting mosquitoes, and more particularly, a mosquito attractant used for attracting, capturing or killing mosquitoes, and the mosquito attracting agent. The present invention relates to a mosquito poison bait and a method of attracting mosquitoes using an agent.

When a mosquito sucks blood, it itches and causes a rash or dermatitis at the blood sucking site. In particular, some mosquitoes are considered to be hygienic harmful insects because they transmit pathogens such as dengue fever, Zika fever, yellow fever, encephalitis, and malaria.

Moss includes a thermal sensor that senses the body temperature of an animal, a taste receptor that senses taste, an olfactory receptor that senses volatile substances such as body odor, and a carbon dioxide receptor that senses carbon dioxide, which is a highly volatile substance. It possesses an excellent chemoreceptor system and is taking various actions. For example, a female mosquito may seek blood before spawning, follow the carbon dioxide and body odor that the animal exhales to approach the animal, detect the body temperature with a thermal sensor, detect the target animal, and suck blood. Are known. In addition, male mosquitoes that do not show blood-sucking behavior and female mosquitoes other than the spawning season feed on nectar of flowers, juice of fruits, sap, etc. in order to ingest sugars and secure energy.

Conventionally, mosquitoes have been captured and killed using a trap, and various methods for attracting mosquitoes to the trap have been studied. For example, a mosquito repellent machine that generates carbon dioxide by a chemical reaction and collects mosquitoes by utilizing the habit of mosquitoes gathering in carbon dioxide has been proposed (Patent Document 1). Further, a flying pest attractant (Patent Document 2) containing an attractant containing vinegar and a fragrance as an active ingredient is used, or a mosquito attractant (Patent Document 3) containing a mixture of lactic acid and methyl lactate as an active ingredient is used. Alternatively, mosquitoes are attracted using a mosquito attractant (Patent Document 4) containing one or more of lysine, isovaleric acid, alanine, androstenol, and sebum.

Japanese Unexamined Patent Publication No. 2000-95623 Japanese Unexamined Patent Publication No. 2013-151470 Japanese Unexamined Patent Publication No. 6-65005 Japanese Unexamined Patent Publication No. 2002-241204

However, it cannot be said that the attractant as previously proposed has sufficient attractiveness, and the development of an attractant capable of attracting mosquitoes more effectively is required.
Therefore, an object of the present invention is to provide a mosquito attractant that exhibits high attractiveness to mosquitoes.

As a result of diligent research, the present inventors have found that the components derived from fish and shellfish and seaweed and the compounds contained therein have an excellent mosquito-attracting action, and have completed the present invention.

That is, the present invention is characterized by the following (1) to (5).
(1) A mosquito attractant comprising at least one selected from the group consisting of a component derived from fish and shellfish, a component derived from seaweed, and a compound contained therein.
(2) The fish and shellfish are characterized by containing organisms classified into at least one class selected from the group consisting of ray-finned fish, malacostracans, bivalvia and head and foot nets (1). The mosquito attractant described in.
(3) The mosquito attractant according to (1) or (2) above, wherein the seaweed contains a seaweed classified into the class Brown algae.
(4) A mosquito poison bait agent containing the mosquito attractant according to any one of (1) to (3) above.
(5) A method for attracting mosquitoes, which comprises attracting mosquitoes using the mosquito attractant according to any one of (1) to (3) above.

Since the mosquito attractant of the present invention can strongly attract mosquitoes, it is possible to efficiently capture or kill mosquitoes.

It is a graph which showed the attracting effect of mosquitoes to each food tested in Test Example 1, (a) is a graph which shows the cumulative total number of flying heads, and (b) is a graph which shows the cumulative total number of needle-seeking behaviors. It is a perspective view which shows the structure of the olfact meter used in each test. It is a figure which shows the result of the test example 2, (a) is a graph which shows the number of invading heads into an attract part of an orfactometer, and (b) is a graph which shows the ratio of the number of invading heads with a control sample of a test sample. be. It is a figure which shows the result of the test example 3, (a) is a graph which shows the result of comparison of a sample 6 and a sample 7, and (b) is a graph which shows the result of having compared a sample 6 and a sample 8. .. It is a figure which shows the result of the test example 4, (a) is a graph which shows the result of comparison of a sample 9 and a control sample, (b) is a graph which shows the result of comparison of a sample 10 and a control sample. .. It is a figure which shows the result of the test example 5, (a) is a graph which shows the result of comparison of a sample 11 and a control sample, (b) is a graph which shows the result of comparison of a sample 12 and a control sample. .. It is a figure which shows the result of the test example 6, (a) is a graph which shows the result of comparison of a sample 13 and a control sample, (b) is a graph which shows the result of comparison of a sample 14 and a control sample. .. It is a figure which shows the result of Test Example 7, (a) is the graph which shows the transition of the invading head of Aedes albopictus, (b) is the graph which shows the transition of the number of invading head of Culex quinquefaque, and (c) is the graph which shows the transition of the number of invading head of Culex quinquefaque. (D) is a graph showing the transition of the number of invading Aedes aegypti. It is a figure which shows the result of the test example 8, and is the graph which shows the invasion number and the invasion rate of Aedes albopictus with respect to samples 15 to 33. It is a figure which shows the result of the test example 9, and is the graph which shows the invasion number and the invasion rate of Aedes albopictus for specimens 12, 16, 21, 29 and 34.

Hereinafter, the mosquito attractant of the present invention and the method for producing the same will be described in detail.

The mosquito attractant of the present invention contains at least one selected from the group consisting of a component derived from fish and shellfish, a component derived from seaweed, and a compound contained therein (hereinafter, also referred to as a specific component of the present invention). do.

(Fish and shellfish-derived components and compounds contained in fish and shellfish-derived components)
Fish and shellfish refer to aquatic animals such as fish and shellfish, and fish and shellfish include, for example, mollusks (Chordate), molluscs (arthropods), molluscs (mollusks), and heads. Includes arthropods (Mollusk phylum).
Ray-finned fish include, for example, flatfish (eg, flatfish, flounder, etc.), perciformes (eg, Thai, Scombridae, hydrangea, kiss, hata, mebaru, etc.), salangidae (eg, smelt). , Scombridae, etc.), Perciformes (eg, Osmeriformes, Salangidae, etc.) and the like. Malacostracans include, for example, Decapods (eg, Sergestidae, Penaeid shrimps, Pandalidae, Pandalidae, King crab, King crab, Majidae, Watari crab, Majidae, Heikegani, Ranina ranina, Mokuzu crab. Family, Sergestidae, etc.), Krill order, etc. are included. Bivalvia includes, for example, Veneridae (eg, Veneridae), Pectinida (Scallops, etc.), Ostreoida (eg, Scallops, etc.) and the like. The cephalopod includes, for example, Squid (eg, Ommastrephidae), Myopsina (eg, Loliginidae), Octopus (eg, Ommastrephidae) and the like.
The fish and shellfish preferably include organisms classified into at least one class selected from the group consisting of these ray-finned fish, malacostracans, bivalvia and cephalopods.

The component derived from fish and shellfish is a component contained in all parts of the body, internal organs, fins, shells, etc. of fish and shellfish, contains a volatile compound, and usually exists as a mixture. The component derived from fish and shellfish and the compound contained in the component can be obtained from the fish and shellfish itself or a processed product of fish and shellfish, and the fish and shellfish and its processed product can also be obtained by a method such as solvent extraction or steam distillation. It can be done, and there is no particular limitation.

Examples of the solvent used for extraction include organic solvents, for example, alcohols having 1 to 4 carbon atoms such as ethanol, methanol, 1-propanol, 2-propanol, 1-butanol and 2-butanol; diethyl ether, tetrahydrofuran and the like. Ethers; ketones such as acetone; hexane; benzene; toluene; tetrachloromethane; dimethyl sulfoxide; phenol; mixtures thereof and the like can be mentioned. Among these, alcohols, ethers, ketones and hexane having 1 to 4 carbon atoms are preferable, and extraction with at least one organic solvent selected from the group consisting of methanol, diethyl ether, acetone and hexane is more preferable. ..

For example, when the seafood is crab (Decapoda), the crab-derived component is a component contained in crab meat (crab meat), crab internal organs (crab miso), crab shell (shell), etc. It contains volatile compounds and usually exists as a mixture.

The type of crab for obtaining the crab-derived component and the compound contained in the crab-derived component is not particularly limited. Examples include red king crab, horsehair crab, snow crab, snow crab, and charybdis japonicus.

And, as a compound contained in a crab-derived component, for example,
Sulfur compounds such as dimethyl disulfide and benzyl methyl sulfide;
Amines such as trimethylamine, 2,5-dimethyl-pyrazine, trimethylpyrazine;
2-butanone, 1-pentene-3-one, 2,3-pentanedione, 3,5-octadien-2-one, 2,3-octanedione, 2-nonanone, 3-decene-2-one, 2- Ketones such as undecanone;
Propanal, 3-methyl-butanal, 2-ethyl-3-methyl-butanal, pentanal, (E) -2-pentenal, hexanal, benzaldehyde, (E) -2-hexenal, heptanal, (E) -4-heptenal , (E, E) -2,4-heptadienal, octanal, (E) -2-octenal, nonanal, (E) -2-nonenal, (E, Z) -2,6-nonadienal, (E, E) Aldehydes such as -2,4-decadienal and (E) -2-dodecenal;
Ethanol, 3-methyl-1-butanol, 1-pentanol, 1-penten-3-ol, (Z) -2-penten-1-ol, 2-methyl-2-penten-1-ol, 1-hexanol , 2-Ethyl-1-hexanol, 1-heptene-4-ol, 1-octanol, 1-octene-3-ol, 1,7-octanediene-3-ol, 2,7-octadien-1-ol, Alcohols such as phenethyl alcohol, (E) -2-nonen-1-ol, 10-undecine-1-ol, 3-tetradecine-1-ol;
Esters such as ethyl acetate and propionic acid octyl ester;
(E) -2-heptene, 3-methylene-heptene, 3-methyl-1,4-heptadiene, 3,3-dimethyl-1,6-heptadiene, (Z) -3-octene-1-ene, (Z) , Z) -3,5-octadiene, 2,7-dimethyl-1,7-octadien, 4-ethyl-octane, 4-ethyl-3-octene, 5-methyl-nonan, decane, 4-ethyl-decane, 2-butyl-1-decane, undecane, 2,5-dimethyl-undecane, 3,8-dimethyl-undecane, dodecane, (E) -5-dodecane, 1,11-dodecane, (E) -2-dodecane- Hydrocarbons of 4-ene, dodecane, 2-methyl-dodecane, 2,4-dimethyldodecane, 3,5-dimethyldodecane, tridecane, (E) -5-tridecane, 7-methyl-tridecane, tetradecane, pentadecane, hexadecane, etc. Examples include hydrogens.
One of these compounds may be contained alone in the mosquito attractant, or two or more of these compounds may be contained.

(Components derived from seaweeds and compounds contained in components derived from seaweeds)
Seaweeds refer to algae that grow in the sea, and seaweeds include, for example, the class Brown algae (Ochrophyta).
The brown algae are seaweeds showing brown color, and include, for example, Fucales (for example, Sargassum family, etc.), Kelps (for example, Kelp family, Alaria crassifolia family, etc.), Ectocarpus silata (for example, Mozuku family, etc.) and the like.
The seaweed preferably contains seaweeds classified as brown algae.

The component derived from seaweed is a component contained in seaweed, contains a volatile compound, and usually exists as a mixture. A component derived from seaweed and a compound contained in the component can be obtained from the seaweed itself or a processed product of seaweed, and the seaweed and its processed product can also be obtained by a method such as solvent extraction or steam distillation. It can be done, and there is no particular limitation. As the solvent used for extraction, the same organic solvent as those described above can be used.

For example, when the seaweed is a brown algae, the brown algae include, for example, hijiki, kelp, wakame seaweed, mozuku, etc., and the components derived from the brown algae are contained therein, and usually contains a volatile compound. It exists as a mixture.

Examples of the compound contained in the brown algae-derived component include hexanal, (E) -2-hexenal, 2-pentylfuran, acetic acid, 5-methyl-2-francarbaldehyde, 2,6-nonazienal, and pyrrole. -2-carboxyaldehyde, hexyl dodecanoate, cubenol, (2E) -nonenal (2), 2-nonenole and tetradecanoic acid, hexanal (3), (2E) -hexenol, (3E) -hexenol, β-cyclocitral (3E) -hexenol, β-cyclocitral (3E) 8), β-homocyclocitral, dihydroactinidiolide (10), dictopterene A (11), B (12), C'(13), D'(14), dictioprolene ( 15), β-ionone (16), (7Z, 10Z) -hexadecadienal, (8Z, 11Z) -heptadecadienal (17), (8Z, 11Z, 14Z) -heptadecatrienal (18). , (8Z) -Heptadecenal (19) and the like.
One of these compounds may be contained alone in the mosquito attractant, or two or more of these compounds may be contained.

Among these, the compound contained in the component derived from brown algae is preferably a compound contained in the component contained in Hijiki, for example, hexanal, (E) -2-hexenal, 2-pentylfuran, acetic acid, 5 -Methyl-2-francarbaldehyde, 2,6-nonazienal, pyrrole-2-carboxyaldehyde, hexyl dodecanoate, cubenol, (2E) -nonenal (2), 2-nonenor and tetradecanoic acid, hexanal (3), ( 2E) -hexenol, (3E) -hexenol, β-cyclocitral (8), β-homocyclocitral, dihydroactinidiolide (10), dictopterene A (11), B (12), C '(13), D'(14), dictioprolene (15), β-ionone (16), (7Z, 10Z) -hexadecadienal, (8Z, 11Z) -heptadecadienal (17) , (8Z, 11Z, 14Z) -Heptadecatorienal (18), (8Z) -Heptadecenal (19) and the like.

The specific component of the present invention, that is, a component derived from fish and shellfish, a component derived from seaweed, and a compound contained therein may be contained alone in the mosquito attractant, or may contain two or more kinds. It may be.

The specific component of the present invention is a volatile component collected by leaving the sample in a closed container for a predetermined time or a component extracted from the sample using a solvent by gas chromatograph mass spectrometer (GC / MS) or liquid chromatograph. It can be identified by separation and quantification by a graph mass spectrometer (LC / MS), analysis by a nuclear magnetic resonance apparatus (NMR), or the like.

Since the mosquito attractant contains the specific component of the present invention, the details are not clear, but the mosquitoes are more likely to be attracted by these, and the attracting effect is enhanced.

In the mosquito attractant, the specific component of the present invention may contain an effective amount capable of attracting mosquitoes, and the content thereof is not particularly limited. The mosquito attractant may be composed of only the specific component of the present invention (100% by mass), or may be contained in a solvent.

The mosquito attractant of the present invention can be obtained, for example, by using a food containing the specific component of the present invention or by mixing the food with another attracting compound or the like.

The mosquito attractant of the present invention includes other known attractant components, insecticidal components, antibacterial components, colorants, ultraviolet absorbers, preservatives, lubricants, and solvents as long as the effect of attracting mosquitoes is not impaired. Etc. can be contained.

Other attracting ingredients include, for example, alcoholic beverages such as brandy, whiskey, lamb liquor, vodka, shochu, and sake; brewed vinegar such as black vinegar, red vinegar, vinegar, apple vinegar, and rice vinegar; honey, liquid sugar, and maple syrup. Sugars such as; vinegar drinks, yogurt, lactic acid products such as cheese; fruit juices; fruit-like fragrances and the like.

Examples of the insecticidal component include dichlorvos, fenitrothione, IBTA, IBTE, transfluthrin, metfurthrin, profluthrin, empentrin, propoxul, phenocarbus, amidoflumeth, dinotefuran, fipronil, hydramethylnon, carbyl, boric acid and the like.

Examples of the antibacterial component include chlorine dioxide, iodine, timol, isopropylmethylphenol, formaldehyde, glutaraldehyde, ethanol, propyl alcohol, phenol, cresol, phenoxyethanol, cetylpyridinium chloride, paraben and the like.

Examples of the colorant include Blue No. 1 and Yellow No. 4.

Examples of the ultraviolet absorber include trisresorcinol triazine compounds and the like.

Examples of the solvent include lower alcohols such as methanol, ethanol and propanol, hydrocarbon solvents such as liquid paraffin, distilled water, tap water, deionized water and the like.

The dosage form of the mosquito attractant of the present invention can be selected according to the usage pattern, and is impregnated with liquid, paste, solid (jelly, powder, granular, etc.), volatilized paper, porous body, etc. The state of being impregnated with the paste can be mentioned.
Specifically, the liquid mosquito attractant can be used as it is, the liquid mosquito attractant and the gelling agent can be mixed to form a paste or solid, or the water-absorbent polymer can be impregnated with the liquid mosquito attractant. Alternatively, a solid mosquito attractant can be obtained by supporting a liquid mosquito attractant on an inorganic carrier.
Further, fish and shellfish or seaweed may be used as they are, boiled in water, dried, baked or the like.

(How to use (attract) mosquito attractants)
The mosquito attractant of the present invention can be filled in a container or impregnated with a fibrous carrier and installed together with a trap. The mosquitoes attracted by the mosquito attractant of the present invention are captured by the trap.

As the catcher, a conventionally known one can be used, and examples thereof include a catcher provided with an adhesive sheet and a catcher using a suction fan that sucks and catches mosquitoes by the attractive force of the wind.

(Mosquitoes poison bait)
The mosquito attractant of the present invention can be used as a mosquito poison bait when used together with a mosquito insecticidal component. That is, the mosquito poison bait of the present invention includes the above-mentioned mosquito attractant, a mosquito insecticidal component, and a mosquito eating component.

The mosquito attractant of the present invention may contain an effective amount capable of attracting mosquitoes in the mosquito poison bait, and the content thereof is not particularly limited and can be appropriately adjusted.

Examples of the insecticidal component of mosquitoes include the above-mentioned insecticidal component. Above all, it is preferable to use dinotefuran, fipronil, boric acid or the like from the viewpoint of not inhibiting the attractiveness of the mosquito attractant.

The shape of the poison bait for mosquitoes is not particularly limited, and examples thereof include liquid, paste, and solid (gel, granular, and powder).

Examples of the mosquitoes to be attracted in the present invention include mosquitoes such as Aedes albopictus and Aedes aegypti, Culex pipiens, Culex quinquefatus, Culex pipiens, Culex mosquito, and Anopheles mosquitoes, but are limited to these exemplary mosquitoes. It's not a thing.

Hereinafter, the present invention will be further described based on specific test examples, but the present invention is not limited to the following examples. Samples 1 to 3 and 6 to 34 are examples, and samples 4 to 5 are comparative examples.

<Test Example 1>
The mosquito attracting effect was confirmed for the following samples 1 to 4.
・ Specimen 1: Crab loosened meat ("Red snow crab loosened meat" (trade name) manufactured by Nippon Suisan Kaisha Co., Ltd.)
-Sample 2: The rehydrated hijiki ("Easy and convenient hijiki" (trade name) manufactured by Yamanaka Foods Co., Ltd.) was soaked in hot water at about 70 ° C for 10 minutes, then soaked in water at room temperature for 10 minutes, and drained. )
・ Specimen 3: Tuna (tuna fillet for sashimi)
・ Specimen 4: Beef tendon (domestic beef tendon)

(Test method)
1. 1. Adult Aedes albopictus (7-10 days old, male-female mixture 1: 1), about 1,200 animals were used as test insects.
2. 2. The test insects were placed in a mesh cage of 25 cm in length × 25 cm in width × 25 cm in height and left in a test room at a temperature of 27 ° C. ± 2 ° C. and a humidity of 60% ± 20% for 1 hour to acclimatize the test insects.
3. 3. About 10 g of the sample was placed on a filter paper of φ7 cm placed on a glass petri dish of φ9 cm, and it was allowed to stand in a mesh cage. A KP cup containing 100 mL of tap water was previously installed in the mesh cage so that the test insects would not react only to the water content of the food.
4. The number of test insects landed on the sample was counted every 30 seconds for 3 minutes after the glass petri dish containing the sample was placed in the mesh cage, and the total number of flying insects was calculated 3 minutes later. Similarly, the number of needle-seeking behaviors, which is one of the blood-sucking behaviors of mosquitoes, was counted every 30 seconds, and the cumulative total number of needle-seeking behaviors was observed after 3 minutes.
The cumulative number is the sum of the total number every 30 seconds.
5. The test was conducted three times, and the data was expressed as the average value of the total number of flying heads and the total number of heads that searched for needles 3 minutes after the start of the test.

The results are shown in FIGS. 1 (a) and 1 (b).

As shown in FIGS. 1 (a) and 1 (b), the cumulative total number of flying heads of Specimen 1 (crab loosened meat), Specimen 2 (Hijiki) and Specimen 3 (Tuna) was larger than that of Specimen 4. It was found that Specimen 1 and Specimen 2 strongly exhibited needle-seeking behavior (behavior of determining the blood-sucking place and piercing the needle), which is one of the blood-sucking behaviors of mosquitoes, and it is presumed that the mosquitoes misidentified them as blood-sucking sources. Was done.

<Test Example 2>
Specimen 1 (crab loosened meat), sample 2 (hijiki) used in Test Example 1, and the following sample 5 were subjected to a comparison test of the attractive effect using an olfactometer.
・ Specimen 5: Honey ("Pure Honey" (trade name) manufactured by Kato Mibeeen Honpo Co., Ltd.)

(Test method)
1. 1. Adult Aedes albopictus (7-10 days old, about 50 females, about 50 males) were used as test insects, and tests were conducted on each of males and females.
2. 2. The test used an olfactometer 10 as shown in FIG. The olfactometer 10 is a set of transparent sample boxes (first sample box 1A, second sample box 1B), and a transparent tubular attraction connected to each of the first sample box 1A and the second sample box 1B. It is provided with portions 2A and 2B and a transparent mosquito containment box 3 connected to the attracting portions 2A and 2B. A check valve 4 is provided at the connecting portion between the mosquito containing box 3 and the attracting portions 2A and 2B to prevent mosquitoes that have invaded the attracting portions 2A and 2B from the mosquito containing box 3 from re-invading the mosquito containing box 3. ing. A net is provided at the connecting portion between the first sample box 1A and the attracting portion 2A and the connecting portion between the second sample box 1B and the attracting portion 2B, respectively, and the mosquitoes invading the attracting portions 2A and 2B are the first specimens. It is designed so that it cannot enter the box 1A and the second sample box 1B. A partition door is provided at the connecting portion to close the odor, and when the test is started, the partition door is opened, clean air is flowed into the olfactometer 10, and the sample boxes 1A and 1B are used. An air flow is generated at 0.3 m / s toward the mosquito containment box 3.
3. 3. The olfactometer 10 was installed in a test room having a temperature of 27 ° C. ± 2 ° C. and a humidity of 60% ± 20%, and the test insects were released into the mosquito containment box 3 in the olfactometer 10 and acclimatized for 1 hour.
4. A test sample containing about 50 g of the sample in a φ9 cm glass petri dish was placed in the first sample box 1A of the orfactometer 10 to form a sample section. In the other second sample box 1B, a φ9 cm glass petri dish containing 50 g of 2% sugar water was placed as a control sample and used as a control group.
5. The partition door of the olfactometer 10 is opened to allow the test insects to invade the attracting parts 2A and 2B, and 10 to 37 hours after the partition door is opened, the test insects invading each attracting part are individual. I counted the number.
6. The test was carried out three times, and the average of the number of animals invading the attracting part and the ratio of the number of animals invading the control sample to the number of animals invading the test sample (number of animals invading the sample group / number of animals invading the control group) was calculated.

The results are shown in FIGS. 3 (a) and 3 (b).

From the results of FIGS. 3 (a) and 3 (b), it can be confirmed that the sample 1 (crab loosening body) shows the strongest attraction to mosquitoes, and the invasion rate to the attracting part is 10 times or more that of the control group. rice field. Further, in Specimen 1 and Specimen 2, it was found that the test insects that invaded the attracting part actively searched for needles, and there is a possibility that the crab loosened meat was misidentified as a blood-sucking source and attracted. .. In Specimen 5 (honey), the mosquitoes did not perform needle-seeking behavior.

<Test Example 3>
The following samples 6 to 8 were subjected to a confirmation test of the attracting effect. The sample 6 is the same as the sample 1.
・ Specimen 6: Crab loosened meat (immediately after opening) (Nippon Suisan Kaisha Co., Ltd. "Red snow crab loosened meat" (trade name), 25 g)
-Sample 7: Crab loosened meat (1 day volatilized product) (Sample 6 left at 27 ° C for 24 hours, 16 g)
-Sample 8: Crab loosened meat (3 days volatilized product) (Sample 6 left at 27 ° C for 3 days, 4 g)

(Test method)
1. 1. Adult Aedes albopictus (7-10 days old, female, about 100) were used as test insects.
2. 2. Approximately 25 g of crab loosened meat of sample 6 was placed in a φ9 cm glass petri dish and left to stand in a test room set at 27 ° C. for a predetermined time without a lid to obtain samples 7 and 8. Specimen 7 is one day (24 hours) after the start of standing (1 day volatilization product), and sample 8 is 3 days (3-day volatilization product).
3. 3. The test was performed using the olfactometer described in Test Example 2. Specimen 7 or Specimen 8 was placed in the first sample box 1A of the olfactometer 10, and Specimen 6 was placed in the other second sample box 1B.
4. The test was carried out in the same manner as in Test Example 2, and the number of test insects invading each attracting part was counted over time after the partition door was opened, and the cumulative total number of invading insects was determined.
5. The test was conducted three times, and the average number of animals invading the attracting part was calculated.

The results are shown in FIGS. 4 (a) and 4 (b).

From the results of FIGS. 4A and 4B, Specimen 6 (immediately after opening) and Specimen 7 (daily volatilized product) showed almost no difference in attractiveness to mosquitoes, but Specimen 8 (3-day volatilized product). ) Was confirmed to attract mosquitoes better than Specimen 6.

<Test Example 4>
The following samples 9 to 10 were subjected to a confirmation test of the attracting effect.
・ Specimen 9: Raw crab meat (collected crab meat from crab leg meat of "Raw Red Snow Crab Stick Potion" (trade name) manufactured by Ocean Foods Co., Ltd., about 20 g)
-Sample 10: Boiled crab meat (crab leg meat used for sample 9 was boiled for 3 minutes and returned to room temperature, and then the crab meat was collected, about 20 g).

(Test method)
1. 1. Adult Aedes albopictus (7-10 days old, female, about 100) were used as test insects.
2. 2. The test was performed using the olfactometer described in Test Example 2. Specimen 9 or Specimen 10 was placed in the first sample box 1A of the olfactometer 10 (sample group), and a φ9 cm glass petri dish containing 20 g of 2% sugar water was placed in the other second sample box 1B (control). Ward).
3. 3. The test was carried out in the same manner as in Test Example 2, and the number of test insects invading each attracting part was counted over time after the partition door was opened, and the cumulative total number of invading insects was determined.
4. The test was conducted three times, and the average number of animals invading the attracting part was calculated.

The results are shown in FIGS. 5 (a) and 5 (b).

From the results of FIGS. 5A and 5B, it was found that mosquitoes were attracted to both the raw crab meat (specimen 9) and the boiled crab meat (specimen 10), and the sample was better than the sample 9. It was found that 10 was more attractive to mosquitoes. In addition, the behavior of mosquitoes was confirmed.

<Test Example 5>
The following samples 11 to 12 were subjected to a confirmation test of the attracting effect. The sample 11 is the same as the sample 1.
・ Specimen 11: Crab loosened meat (“Red snow crab loosened meat” (trade name) manufactured by Nippon Suisan Kaisha Co., Ltd., 20 g)
-Sample 12: Crab miso ("Crab miso with meat" (trade name) manufactured by Maruha Nichiro Co., Ltd., 20 g)

The test was carried out in the same manner as in Test Example 4. The results are shown in FIGS. 6 (a) and 6 (b).

From the results of FIG. 6 (b), the mosquitoes invaded the attracting part of the sample 12 (crab miso) immediately after the start of the test, and the high attraction to the mosquitoes was the same as that of the sample 11 (crab loosening body) shown in FIG. 6 (a). It turned out to have sex. In addition, as with the sample 11, it was confirmed that the mosquitoes actively searched for the sample 12 as well.

<Test Example 6>
The following samples 13 to 14 were subjected to a confirmation test of the attracting effect.
-Sample 13: Crab miso ("Crab miso" (trade name) manufactured by Maruyo Foods Co., Ltd., 20 g)
Specimen 14: Returned hijiki ("Hijiki" (trade name) manufactured by Marumo Co., Ltd., soaked in hot water at about 70 ° C for 10 minutes, then soaked in water at room temperature for 10 minutes, drained, 20 g)

The test was carried out in the same manner as in Test Example 4. The results are shown in FIGS. 7 (a) and 7 (b).

From the results of FIGS. 7 (a) and 7 (b), it was confirmed that both the sample 13 (crab miso) and the sample 14 (hijiki) have high attraction to mosquitoes.

<Test Example 7>
A confirmation test of the attracting effect of each type of mosquito was conducted on the sample 12 (“Crab with crab meat” (trade name) manufactured by Maruha Nichiro Co., Ltd., 20 g) used in Test Example 5.
The test insects used are as follows.
・ Adult Aedes albopictus: 7-10 days old, female, about 100 ・ Adult Aedes aegypti: 7-10 days old, female, about 100 ・ Culex pipiens: 7-10 days old, female, about 100 ・ Adult Aedes aegypti: 7 ~ 10 days old, female, about 100

(Test method)
1. 1. The test was performed using the olfactometer described in Test Example 2. A sample 12 was placed in the first sample box 1A of the olfactometer 10 (sample group), and a φ9 cm glass petri dish containing 20 g of 2% sugar water was placed in the other second sample box 1B (control group).
2. 2. The test was conducted in the same manner as in Test Example 2, and the number of test insects invading each attracting part was counted over time for 9 to 25 hours after the cut door was opened, and the cumulative total number of invading animals was counted. Asked. Since Culex is blood-sucking at night, the test was conducted continuously for one night or longer.
3. 3. The test was conducted three times, and the average number of animals invading the attracting part was calculated.

The results are shown in FIGS. 8 (a) to 8 (d).

From the results of FIGS. 8 (a) to 8 (d), it was found that there is an attractive effect not only on Aedes albopictus but also on Aedes aegypti, Culex pipiens, and Culex quinquefatus. Among them, high attractiveness was confirmed for Aedes albopictus and Aedes aegypti, and it was found that it exerts an excellent attracting effect for mosquitoes of the genus Aedes, which is Aedes.

<Test Example 8>
The following samples 15 to 33 were subjected to a confirmation test of the attracting effect.
・ Specimen 15: Whiteleg shrimp (“Thawed whiteleg shrimp from India (cultured)” (processed product of Daikokuten Bussan Co., Ltd.))
・ Specimen 16: Pink shrimp ("Hyogo prefecture-produced sweet shrimp" (processed product of Daikokuten Bussan Co., Ltd.))
-Sample 17: Akiami ("G Ami Ebi" (trade name) manufactured by HAMAICHI)
-Sample 18: Krill (Koharu's "Tsurimonogatari Seigoku S size" (trade name))
・ Specimen 19: Abra flatfish ("Aburakarei" (processed product of Daikokuten Bussan Co., Ltd.))
・ Specimen 20: Red sea bream (“Olive aged red sea bream strip for sashimi (culture)” (processed product of Daikokuten Bussan Co., Ltd.))
・ Specimen 21: Coho salmon (“Chilean silver salmon fillet (cultured) thawed” (processed product of Daikokuten Bussan Co., Ltd.))
・ Specimen 22: Yellowfin ("For sashimi from Kagoshima prefecture (thawed)" (processed product of Daikokuten Bussan Co., Ltd.))
・ Specimen 23: Yellowtail ("Nagasaki Prefecture Natural Buri Fillet" (processed product of Daikokuten Bussan Co., Ltd.))
・ Specimen 24: Chub mackerel (“Grated mackerel from Wakayama prefecture” (processed product of Daikokuten Bussan Co., Ltd.))
・ Specimen 25: Horse mackerel (“Small horse mackerel from Kochi prefecture” (processed product of Daikokuten Bussan Co., Ltd.))
・ Specimen 26: Ayu (“Ayu from Gifu Prefecture (cultured)” (processed product of Daikokuten Bussan Co., Ltd.))
・ Specimen 27: Satsukimasu (“Cultured Amago from Tokushima Prefecture” (processed product of Daikokuten Bussan Co., Ltd.))
・ Specimen 28: Oysters (“raw oysters” (obtained from Hotaka Yamashita Shoten Co., Ltd.))
-Sample 29: Asari ("Peeled meat of Asari (for heating)" (processed product of Kamoi Food Industry Co., Ltd.))
・ Specimen 30: Scallop ("Scallop for sashimi from Hokkaido" (processed product of Hinase Shopping Center Co., Ltd.)
-Sample 31: Japanese flying squid ("Chinese flying squid (for thawing and heating)" (processed product of Yoshida Shoji Co., Ltd.))
・ Specimen 32: Squid ("Yariika from Miyagi Prefecture" (processed product of AEON Retail Co., Ltd.))
・ Specimen 33: Common octopus (“Steamed octopus (steamed octopus) using Moroccan raw materials for sashimi” (processed product of AEON Retail Co., Ltd.))

(Test method)
1. 1. Adult Aedes albopictus (7-10 days old, female, about 100) were used as test insects.
2. 2. The test was performed using the olfactometer described in Test Example 2. A sample 20 g was placed in the first sample box 1A of the olfactometer 10 (sample group), and a φ9 cm glass petri dish containing 20 g of 2% sugar water was placed in the other second sample box 1B (control group).
3. 3. The test was carried out in the same manner as in Test Example 2, and the number of test insects invading each attracting part was counted at an arbitrary time 8 to 11 hours after the partition door was opened.
4. The test was carried out three times, and the average value of the number of animals invading the attracting part and the invasion rate (%) into the attracting part on the sample group side was determined.
Invasion rate to the sample section side attraction section (%) = Number of invading animals to the sample section side attraction section / (Number of invasion to the sample section side attraction section + Number of invasion to the control section side attraction section) x 100

The results are shown in Table 1 and FIG. In the graph of FIG. 9, the first vertical axis on the left side is the number of animals invading the attracting portion, and the second vertical axis on the right side is the invasion rate to the attracting portion on the sample section side.

From the results of Table 1 and FIG. 9, all the samples had an invasion rate of 75% or more in the attracting part on the sample group side, and showed high attracting property to mosquitoes. Among them, sample 15 (whiteleg shrimp), sample 16 (pink shrimp), sample 18 (krill), sample 21 (coho salmon), sample 22 (yellowfin), sample 25 (horse mackerel), sample 27 (satsukimasu), sample 29 (sari) The invasion rate to the attracting part was extremely high at 90% or more.

<Test Example 9>
Extracts for sample 12 (crab miso) used in test example 5, sample 16 (pink shrimp) used in test example 8, sample 21 (coho salmon) and sample 29 (casari), and sample 34 (crab meat) below. We confirmed the attraction effect in.
-Sample 12: Crab miso ("Crab miso with meat" manufactured by Maruha Nichiro Co., Ltd. (trade name)
・ Specimen 16: Pink shrimp ("Hyogo prefecture-produced sweet shrimp" (processed product of Daikokuten Bussan Co., Ltd.))
・ Specimen 21: Coho salmon (“Chilean silver salmon fillet (cultured) thawed” (processed product of Daikokuten Bussan Co., Ltd.))
-Sample 29: Asari ("Peeled meat of Asari (for heating)" (processed product of Kamoi Food Industry Co., Ltd.))
-Sample 34: Crab meat (Fukui Canned Co., Ltd. "Red Snow Crab (Flake)" (trade name))

(Preparation of extract)
Specimen 16 (pink shrimp), sample 21 (coho salmon) and sample 29 (sari) were ground using a food processor to make surimi.
Approximately 20 g of each of the surimi of the sample 12 (crab miso), the sample 16 (pink shrimp), the surimi of the sample 21 (coho salmon) and the surimi of the sample 29 (lajonkairia lajonii) were weighed on a cylindrical filter paper and set in a Soxhlet extractor.
About 25 g of the sample 34 (crab meat) was weighed in a glass petri dish of φ9 cm, left to stand at 27 ° C. for 3 days, transferred to a cylindrical filter paper, and set in a Soxhlet extractor.
Hexane and methanol were refluxed at about 80 ° C., acetone at about 70 ° C., and diethyl ether at about 40 ° C. for about 5 hours, respectively, to extract solvent-soluble components from each sample. After completion of reflux, the pressure was reduced using an evaporator, and the mixture was concentrated under 40 ° C. conditions until no boiling of the solution was observed to obtain an extract.

(Test method)
The test was performed using the olfactometer described in Test Example 2.
The extract obtained above depends on the extraction solvent of the extract so that the amount of the compound contained in the extract to be tested in one test is the same as the amount of the compound contained in 20 g of fish and shellfish before extraction. Dilution and adjustment of the test extract treatment amount were performed.
Hexane, diethyl ether, acetone and methanol extracts of crab miso and crab meat, and hexane and diethyl ether extracts of scallop shrimp and ginkgo biloba contain compounds contained in 20 g of fish and shellfish before extraction per 3 g after dilution with an extraction solvent. It was diluted so as to be used for the test. An extraction solvent of 3 g was placed in the control group.
Acetone and methanol extracts of northern shrimp and pink shrimp were tested by adjusting the amount of the compound so as to be the same as the amount of the compound contained in 20 g of fish and shellfish before extraction without diluting with an extraction solvent. 20 g of 2% sugar water was installed in the control plot.

Adult Aedes albopictus (7-10 days old, female, about 100) were used as test insects. The test was carried out in the same manner as in Test Example 8, and the number of test insects invading each attracting part was counted at an arbitrary time 7 to 12 hours after the partition door was opened.
The test was carried out three times, and the average value of the number of animals invading the attracting part and the invasion rate (%) into the attracting part on the sample group side was determined.

The results are shown in Table 2 and FIG. In the graph of FIG. 10, the first vertical axis on the left side is the number of animals invading the attracting portion, and the second vertical axis on the right side is the invasion rate to the attracting portion on the sample section side.

From the results of Table 2 and FIG. 10, the invasion rate of mosquitoes into the sample group was equal to or higher than that of the control group for all the samples, and the extracts of hexane, diethyl ether, acetone or methanol were all mosquitoes. It turned out to be attractive to. This suggests that these extracts contain compounds that attract mosquitoes.

1A 1st sample box 1B 2nd sample box 2A, 2B Attracting part 3 Mosquito containment box 4 Check valve 10 Orfact meter

Claims (5)

A mosquito attractant comprising at least one selected from the group consisting of a component derived from fish and shellfish, a component derived from seaweed, and a compound contained therein. The mosquito according to claim 1, wherein the fish and shellfish include an organism classified into at least one class selected from the group consisting of a class of sardines, malacostracans, bivalvia and head and foot nets. Class attractant. The mosquito attractant according to claim 1 or 2, wherein the seaweed contains a seaweed classified into the class Brown algae. A mosquito poison bait agent containing the mosquito attractant according to any one of claims 1 to 3. A method for attracting mosquitoes, which comprises attracting mosquitoes using the mosquito attractant according to any one of claims 1 to 3.

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