JP2021530549A - タンパク質における翻訳後修飾の、単一分子配列決定による同定 - Google Patents

タンパク質における翻訳後修飾の、単一分子配列決定による同定 Download PDF

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JP2021530549A
JP2021530549A JP2021503788A JP2021503788A JP2021530549A JP 2021530549 A JP2021530549 A JP 2021530549A JP 2021503788 A JP2021503788 A JP 2021503788A JP 2021503788 A JP2021503788 A JP 2021503788A JP 2021530549 A JP2021530549 A JP 2021530549A
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peptide
protein
post
amino acid
translational modification
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JP2021530549A5 (https=
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エドワード マーコット
エリック アンスリン
ジャガナス スワミナサン
アンジェラ エム． バルド
キャロライン エム． ヒンソン
セシル ハワード
ブレンダン フロイド
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University of Texas System
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University of Texas System
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818—Sequencing of polypeptides
    • G01N33/6824—Sequencing of polypeptides involving N-terminal degradation, e.g. Edman degradation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818—Sequencing of polypeptides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2440/00—Post-translational modifications [PTMs] in chemical analysis of biological material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2440/00—Post-translational modifications [PTMs] in chemical analysis of biological material
    • G01N2440/12—Post-translational modifications [PTMs] in chemical analysis of biological material alkylation, e.g. methylation, (iso-)prenylation, farnesylation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2440/00—Post-translational modifications [PTMs] in chemical analysis of biological material
    • G01N2440/14—Post-translational modifications [PTMs] in chemical analysis of biological material phosphorylation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2440/00—Post-translational modifications [PTMs] in chemical analysis of biological material
    • G01N2440/18—Post-translational modifications [PTMs] in chemical analysis of biological material citrullination
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2440/00—Post-translational modifications [PTMs] in chemical analysis of biological material
    • G01N2440/26—Post-translational modifications [PTMs] in chemical analysis of biological material nitrosylation

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2021503788A 2018-07-23 2019-07-23 タンパク質における翻訳後修飾の、単一分子配列決定による同定 Pending JP2021530549A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862702318P 2018-07-23 2018-07-23
US62/702,318 2018-07-23
PCT/US2019/042998 WO2020023488A1 (en) 2018-07-23 2019-07-23 Single molecule sequencing identification of post-translational modifications on proteins

Publications (2)

Publication Number Publication Date
JP2021530549A true JP2021530549A (ja) 2021-11-11
JP2021530549A5 JP2021530549A5 (https=) 2022-08-01

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JP2021503788A Pending JP2021530549A (ja) 2018-07-23 2019-07-23 タンパク質における翻訳後修飾の、単一分子配列決定による同定

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Country Link
US (1) US20210215706A1 (https=)
EP (1) EP3827093A4 (https=)
JP (1) JP2021530549A (https=)
CN (1) CN112469832A (https=)
WO (1) WO2020023488A1 (https=)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2839702C (en) 2011-06-23 2021-04-20 Board Of Regents, The University Of Texas System Identifying peptides at the single molecule level
US11435358B2 (en) 2011-06-23 2022-09-06 Board Of Regents, The University Of Texas System Single molecule peptide sequencing
CA3208970A1 (en) 2014-09-15 2016-05-06 Board Of Regents, The University Of Texas System Improved single molecule peptide sequencing
CA3110800A1 (en) 2018-07-12 2020-01-16 Board Of Regents, The University Of Texas System Molecular neighborhood detection by oligonucleotides
CA3117476A1 (en) 2018-10-05 2020-04-09 Board Of Regents, The University Of Texas System Solid-phase n-terminal peptide capture and release
AU2019380606B2 (en) 2018-11-15 2026-04-23 Quantum-Si Incorporated Methods and compositions for protein sequencing
EP4045684A1 (en) * 2019-10-28 2022-08-24 Quantum-Si Incorporated Methods of preparing an enriched sample for polypeptide sequencing
WO2021236716A2 (en) * 2020-05-19 2021-11-25 Board Of Regents, The University Of Texas System Methods, systems and kits for polypeptide processing and analysis
EP4143579A2 (en) 2020-05-20 2023-03-08 Quantum-si Incorporated Methods and compositions for protein sequencing
CN116270639B (zh) * 2023-03-31 2025-08-22 中山大学 N-(2-(5-(1，2-二硫戊环-3-基)戊酰胺基)乙基)烟酰胺的新应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150087526A1 (en) * 2012-01-24 2015-03-26 The Regents Of The University Of Colorado, A Body Corporate Peptide identification and sequencing by single-molecule detection of peptides undergoing degradation

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US20040053356A1 (en) * 2001-12-28 2004-03-18 Mds Proteomics Inc. Enzyme/chemical reactor based protein processing method for proteomics analysis by mass spectrometry
CA2545685A1 (en) * 2003-11-14 2005-06-02 Irm Llc Fluorous labeling for selective processing of biologically-derived samples
US7476656B2 (en) * 2004-11-09 2009-01-13 University Of Florida Research Foundation, Inc. Fluorescent affinity tag to enhance phosphoprotein detection and characterization
BRPI0617191A2 (pt) * 2005-10-12 2011-07-19 Scripps Research Inst modificação pós-traducional de polipeptìdeos expressos em fagos
WO2010044892A1 (en) * 2008-10-17 2010-04-22 President And Fellows Of Harvard College Diagnostic method based on large scale identification of post-translational modification of proteins
HRP20161787T1 (hr) * 2011-02-16 2017-02-24 Cellseed Inc. Sredstvo za označavanje za post-translacijsku modifikacijsku analizu serina i treonina
CN109642231A (zh) * 2016-06-17 2019-04-16 博德研究所 Vi型crispr直向同源物和系统

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150087526A1 (en) * 2012-01-24 2015-03-26 The Regents Of The University Of Colorado, A Body Corporate Peptide identification and sequencing by single-molecule detection of peptides undergoing degradation

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ANAL.CHEM., vol. 75, no. 24, JPN6023025430, 2003, pages 6826 - 6836, ISSN: 0005384207 *
BIOPOLYMERS, vol. 101, no. 2, JPN6023025431, 2014, pages 173 - 179, ISSN: 0005384208 *
CHEM REV., vol. 116, no. 23, JPN6023025428, 2016, pages 14277 - 14306, ISSN: 0005384210 *
METHODS, vol. 62, JPN6023025432, 2013, pages 138 - 150, ISSN: 0005384209 *
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, JPN6023025429, 2005, pages 2157 - 2162, ISSN: 0005384206 *

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Publication number Publication date
CN112469832A (zh) 2021-03-09
EP3827093A4 (en) 2022-10-05
WO2020023488A1 (en) 2020-01-30
EP3827093A1 (en) 2021-06-02
US20210215706A1 (en) 2021-07-15

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