CN112469832A - 蛋白质上的翻译后修饰的单分子测序鉴别 - Google Patents

蛋白质上的翻译后修饰的单分子测序鉴别 Download PDF

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Publication number
CN112469832A
CN112469832A CN201980048949.XA CN201980048949A CN112469832A CN 112469832 A CN112469832 A CN 112469832A CN 201980048949 A CN201980048949 A CN 201980048949A CN 112469832 A CN112469832 A CN 112469832A
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peptide
protein
post
amino acid
translational modification
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Pending
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CN201980048949.XA
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English (en)
Chinese (zh)
Inventor
E·马科特
E·安斯林
J·斯瓦米纳坦
A·M·巴尔多
C·M·辛森
C·霍华德
B·弗洛伊德
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University of Texas System
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University of Texas System
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Publication of CN112469832A publication Critical patent/CN112469832A/zh
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818—Sequencing of polypeptides
    • G01N33/6824—Sequencing of polypeptides involving N-terminal degradation, e.g. Edman degradation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818—Sequencing of polypeptides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2440/00—Post-translational modifications [PTMs] in chemical analysis of biological material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2440/00—Post-translational modifications [PTMs] in chemical analysis of biological material
    • G01N2440/12—Post-translational modifications [PTMs] in chemical analysis of biological material alkylation, e.g. methylation, (iso-)prenylation, farnesylation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2440/00—Post-translational modifications [PTMs] in chemical analysis of biological material
    • G01N2440/14—Post-translational modifications [PTMs] in chemical analysis of biological material phosphorylation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2440/00—Post-translational modifications [PTMs] in chemical analysis of biological material
    • G01N2440/18—Post-translational modifications [PTMs] in chemical analysis of biological material citrullination
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2440/00—Post-translational modifications [PTMs] in chemical analysis of biological material
    • G01N2440/26—Post-translational modifications [PTMs] in chemical analysis of biological material nitrosylation

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201980048949.XA 2018-07-23 2019-07-23 蛋白质上的翻译后修饰的单分子测序鉴别 Pending CN112469832A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862702318P 2018-07-23 2018-07-23
US62/702318 2018-07-23
PCT/US2019/042998 WO2020023488A1 (en) 2018-07-23 2019-07-23 Single molecule sequencing identification of post-translational modifications on proteins

Publications (1)

Publication Number Publication Date
CN112469832A true CN112469832A (zh) 2021-03-09

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CN201980048949.XA Pending CN112469832A (zh) 2018-07-23 2019-07-23 蛋白质上的翻译后修饰的单分子测序鉴别

Country Status (5)

Country Link
US (1) US20210215706A1 (https=)
EP (1) EP3827093A4 (https=)
JP (1) JP2021530549A (https=)
CN (1) CN112469832A (https=)
WO (1) WO2020023488A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116270639A (zh) * 2023-03-31 2023-06-23 中山大学 N-(2-(5-(1，2-二硫戊环-3-基)戊酰胺基)乙基)烟酰胺的新应用

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2839702C (en) 2011-06-23 2021-04-20 Board Of Regents, The University Of Texas System Identifying peptides at the single molecule level
US11435358B2 (en) 2011-06-23 2022-09-06 Board Of Regents, The University Of Texas System Single molecule peptide sequencing
CA3208970A1 (en) 2014-09-15 2016-05-06 Board Of Regents, The University Of Texas System Improved single molecule peptide sequencing
CA3110800A1 (en) 2018-07-12 2020-01-16 Board Of Regents, The University Of Texas System Molecular neighborhood detection by oligonucleotides
CA3117476A1 (en) 2018-10-05 2020-04-09 Board Of Regents, The University Of Texas System Solid-phase n-terminal peptide capture and release
AU2019380606B2 (en) 2018-11-15 2026-04-23 Quantum-Si Incorporated Methods and compositions for protein sequencing
EP4045684A1 (en) * 2019-10-28 2022-08-24 Quantum-Si Incorporated Methods of preparing an enriched sample for polypeptide sequencing
WO2021236716A2 (en) * 2020-05-19 2021-11-25 Board Of Regents, The University Of Texas System Methods, systems and kits for polypeptide processing and analysis
EP4143579A2 (en) 2020-05-20 2023-03-08 Quantum-si Incorporated Methods and compositions for protein sequencing

Citations (4)

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US20040053356A1 (en) * 2001-12-28 2004-03-18 Mds Proteomics Inc. Enzyme/chemical reactor based protein processing method for proteomics analysis by mass spectrometry
US20060099604A1 (en) * 2004-11-09 2006-05-11 The University Of Florida Research Foundation, Inc. Fluorescent affinity tag to enhance phosphoprotein detection and characterization
CN101287830A (zh) * 2005-10-12 2008-10-15 斯克利普斯研究院 噬菌体展示多肽的选择性翻译后修饰
US20150087526A1 (en) * 2012-01-24 2015-03-26 The Regents Of The University Of Colorado, A Body Corporate Peptide identification and sequencing by single-molecule detection of peptides undergoing degradation

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CA2545685A1 (en) * 2003-11-14 2005-06-02 Irm Llc Fluorous labeling for selective processing of biologically-derived samples
WO2010044892A1 (en) * 2008-10-17 2010-04-22 President And Fellows Of Harvard College Diagnostic method based on large scale identification of post-translational modification of proteins
HRP20161787T1 (hr) * 2011-02-16 2017-02-24 Cellseed Inc. Sredstvo za označavanje za post-translacijsku modifikacijsku analizu serina i treonina
CN109642231A (zh) * 2016-06-17 2019-04-16 博德研究所 Vi型crispr直向同源物和系统

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040053356A1 (en) * 2001-12-28 2004-03-18 Mds Proteomics Inc. Enzyme/chemical reactor based protein processing method for proteomics analysis by mass spectrometry
US20060099604A1 (en) * 2004-11-09 2006-05-11 The University Of Florida Research Foundation, Inc. Fluorescent affinity tag to enhance phosphoprotein detection and characterization
CN101287830A (zh) * 2005-10-12 2008-10-15 斯克利普斯研究院 噬菌体展示多肽的选择性翻译后修饰
US20150087526A1 (en) * 2012-01-24 2015-03-26 The Regents Of The University Of Colorado, A Body Corporate Peptide identification and sequencing by single-molecule detection of peptides undergoing degradation

Non-Patent Citations (2)

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Title
FRANZISKA DOLL 等: "Visualization of Protein-Specific Glycosylation inside Living Cells", ANGEW. CHEM. INT. ED, vol. 55, pages 2262, XP055779670, DOI: 10.1002/anie.201503183 *
苏坚 等: "蛋白质组学研究进展及其在肿瘤中的应用", 医学分子生物学杂志, vol. 1, no. 5, pages 295 - 297 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116270639A (zh) * 2023-03-31 2023-06-23 中山大学 N-(2-(5-(1，2-二硫戊环-3-基)戊酰胺基)乙基)烟酰胺的新应用
CN116270639B (zh) * 2023-03-31 2025-08-22 中山大学 N-(2-(5-(1，2-二硫戊环-3-基)戊酰胺基)乙基)烟酰胺的新应用

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EP3827093A4 (en) 2022-10-05
JP2021530549A (ja) 2021-11-11
WO2020023488A1 (en) 2020-01-30
EP3827093A1 (en) 2021-06-02
US20210215706A1 (en) 2021-07-15

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