JP2021168703A - Oral composition - Google Patents

Abstract

To provide a composition that can effectively prevent and/or improve high blood pressure.SOLUTION: An oral composition contains pepper plants such as Piper longum, Capsicum annuum, Persicaria hydropiper, or Piper nigrum, and vitamins or polyphenols.SELECTED DRAWING: Figure 1

Description

The present invention relates to an oral composition capable of preventing and / or ameliorating hypertension.

Hypertension is a common disease in Japan, and the number of patients is increasing due to the aging of the population and the westernization of eating habits. It is known that as blood pressure rises, diseases related to the brain and heart are more likely to occur.

As a composition for improving such blood pressure, for example, a hypertension inhibitor (see Patent Document 1) containing a lactic acid bacterium fermented product obtained by a specific method as an active ingredient, or a long-chain hydrocarbon having 16 or more carbon atoms is used. A hypertension improving agent (see Patent Document 2) as an active ingredient has been proposed.

Japanese Unexamined Patent Publication No. 2013-32347 JP-A-2010-248878

An object of the present invention is to provide a composition having an excellent preventive and / or ameliorating effect on hypertension.

As a result of diligent research and study on the functions of pepper plants such as long pepper and green pepper, the present inventors have found that pepper plants have a dose-dependent ability to inhibit ACE (angiotensin converting enzyme) activity. As a result of further research, the present inventors can enhance the ability to inhibit ACE activity by adding specific components such as vitamins and polyphenols to this pepper plant, which is extremely excellent in preventing hypertension and / or. Alternatively, they have found that an improvement effect can be obtained, and have completed the present invention.

That is, the present invention relates to an oral composition comprising a pepper plant and vitamins or polyphenols.

The pepper plant used in the oral composition of the present invention is preferably at least one selected from water pepper, long pepper, green pepper, chili pepper, and black pepper.

The vitamins used in the oral composition of the present invention are vitamin B1 hydrochloride, vitamin B2, vitamin B6, vitamin B12, nicotinic acid, sodium D-pantothenate, biotin, folic acid, vitamin C, vitamin A, vitamin D3, vitamins. It is preferably at least one selected from E and Vitamin K.

The polyphenol used in the oral composition of the present invention is preferably at least one selected from gallic acid, chlorogenic acid, and catechin.

The oral composition of the present invention is preferably in the form of tablets, capsules, powders, granules, or liquids.

The oral composition of the present invention is preferably a composition for preventing and improving hypertension for preventing and / or ameliorating hypertension, and is, for example, a functional food.

The oral composition of the present invention relates to a composition for preventing and improving hypertension containing a pepper plant as an active ingredient, and further comprising vitamins or polyphenols for preventing and improving hypertension. Vitamins or polyphenols exert excellent effects even when the amount is smaller than that of pepper plants.

The oral composition of the present invention relates to a composition for promoting prevention and improvement of hypertension containing vitamins or polyphenols, which is characterized by containing a pepper plant as an active ingredient.

The oral composition of the present invention relates to a composition for preventing and improving hypertension containing vitamins or polyphenols that promote the binding of angiotensin converting enzyme of pepper plants to the active site.

The present invention relates to a method for producing a composition for preventing and improving hypertension, which comprises blending vitamins or polyphenols in an oral composition containing a pepper plant.

According to the oral composition of the present invention, ACE activity can be inhibited and hypertension can be prevented and / or ameliorated.

It is a figure which shows the result of the ACE inhibition rate at the time of applying the composition (long pepper + vitamins or polyphenol) of this invention. It is a figure which shows the result of the ACE inhibition rate when the composition of this invention (green pepper + vitamins or polyphenol) is applied. It is a figure which shows the result of the ACE inhibition rate at the time of applying the composition (water pepper + vitamins or polyphenol) of this invention. It is a figure which shows the result of the ACE inhibition rate when the composition of this invention (chili pepper + vitamins or polyphenol) is applied. It is a figure which shows the result of the ACE inhibition rate at the time of applying the composition (black pepper + vitamins or polyphenol) of this invention.

The oral composition of the present invention is characterized by containing a pepper plant and vitamins or polyphenols. In the composition of the present invention, both vitamins and polyphenols may be contained, and each component of vitamins and polyphenols may be used in combination of two or more. When two or more kinds are used in combination, it is preferable to combine vitamins or polyphenols having a high synergistic effect with pepper plants.

The composition of the present invention can enhance the ability to inhibit ACE activity and obtain an excellent blood pressure improving effect by combining a pepper plant and vitamins or polyphenols, and can more effectively prevent or improve hypertension. Therefore, the composition of the present invention can be used as an ACE activity inhibitor.

[Pepper plant]
The pepper plant used in the composition of the present invention may be any part of the plant such as above-ground part, root part, fruit, etc., and in addition to the plant itself, its dried product, dried crushed product, juice, extract, fermentation. Plant-treated products such as products can be used. Examples of the pulverized product include powder, granules and the like. The juice or extract may be in liquid form, but may also be used as a paste or dry powder. The extract can be obtained by extracting with an appropriate solvent, and as the solvent, for example, water (hot water, hot water), ethanol, or hydrous ethanol can be used. As the pepper plant, a commercially available one may be used.

(Long pepper: scientific name Piper longum)
Long pepper is a plant of the Piperaceae family and is a vine evergreen tree. The fruit has a pungent flavor and spiciness, and has been used as a spice and seasoning by drying the fruit for a long time. Fruits are preferably used as the site used in the composition of the present invention.

(Green pepper: scientific name Capsicum annuum)
Green pepper is a plant of the Solanaceae family, which is a perennial (annual in the temperate zone) or a shrub. A sweet variety of chili pepper, the fruits have a unique flavor, and the immature fruits of green pepper are mainly used as spices and vegetables. As the site used in the composition of the present invention, it is preferable to use fruits, and it is particularly preferable to use immature fruits.

(Water pepper: Scientific name Persicaria hydropiper)
Water pepper is a plant of the Polygonaceae family, which is riparian and annual, and has the characteristic of not pulling rhizomes. Water pepper has a peculiar aroma and spiciness, and is used as a spice for condiments and sashimi. As the site used in the composition of the present invention, it is preferable to use a sprout.

(Chili pepper: scientific name Capsicum annuum)
Chili peppers are plants of the Solanaceae family and are perennials (annuals in the temperate zone) or shrubs. The fruits have a pungent pungent taste and are mainly used as spices by drying the fruits. Fruits are preferably used as the site used in the composition of the present invention.

(Black pepper: scientific name Piper nigrum)
Black pepper is a plant of the Piperaceae family and is a vine evergreen tree. The fruit has a unique flavor and is mainly used as a spice by drying the fruit. As the site used in the composition of the present invention, it is preferable to use fruits, and it is particularly preferable to use immature fruits.

[Vitamins or polyphenols]
(Vitamins)
In the oral composition of the present invention, it is preferable to use vitamins together with the pepper plant. Vitamins include vitamin B1 hydrochloride, vitamin B2, vitamin B6, vitamin B12, nicotinic acid, sodium D-pantothenate, biotin, folic acid, vitamin C (above, water-soluble vitamins), vitamin A, vitamin D3, vitamin E. , And at least one selected from vitamin K1 (above, fat-soluble vitamin) is preferably used.

(Polyphenol)
In the oral composition of the present invention, it is preferable to use polyphenols together with pepper plants. As the polyphenol, it is preferable to use at least one selected from gallic acid, chlorogenic acid, and catechin.

These vitamins or polyphenols function as promoters of the preventive and / or ameliorating function of hypertension in pepper plants.

The oral composition of the present invention includes, for example, functional foods whose efficacy has been approved by a predetermined organization such as pharmaceuticals (including non-pharmaceutical products), foods for specified health uses, foods with nutritional function, foods with functional claims, etc. So-called health foods such as, general foods, food additives, feeds and the like can be mentioned. The oral composition of the present invention can be ingested not only by people with hypertension but also by people who are concerned about hypertension, regardless of gender or age.

The oral composition of the present invention can be used as a composition for preventing and improving hypertension used for preventing and / or improving hypertension, and such a composition for preventing and improving hypertension contains pepper plants and predetermined vitamins or polyphenols. It is not particularly limited as long as it is contained and can be distinguished from other products as a product in that it is used for prevention and / or amelioration of hypertension. For example, the scope of the present invention includes a product that indicates that the main body, packaging, instruction manual, or promotional material of the product according to the present invention has a function of preventing and / or ameliorating hypertension. In the composition for preventing and improving hypertension of the present invention, the combination component (pepper plant and vitamins or polyphenol) of the present invention is displayed as an active ingredient of the preventive and / or improving effect of hypertension on the packaging of the product. It is not limited to what you are doing. For example, the active ingredient may not be specified, pepper plants and vitamins or polyphenols may be labeled as active ingredients, or only pepper plants may be labeled as active ingredients. good.

Specific examples of the composition for preventing and improving high blood pressure include pharmaceuticals and so-called health foods. In so-called health foods, "for those with high blood pressure", "for those who are concerned about blood pressure", and "normal blood pressure". "Help maintain high blood pressure", "Lower high blood pressure", "Improve blood pressure in high blood pressure", "Lower blood pressure in high blood pressure", "Lower blood pressure in high blood pressure" "Helping people with high blood pressure", "Functions suitable for people with high blood pressure", "Functions to lower blood pressure", "Supporting healthy blood pressure for people with high blood pressure", "Helping people with high blood pressure" ”Etc. can be exemplified.

The form of the oral composition of the present invention includes, for example, tablets, capsules, powders, granules, liquids, granules, rods, plates, blocks, solids, rounds, pastes, creams, and caplets. Examples include a shape, a gel shape, a chewable shape, and a stick shape. Among these, tablet-like, capsule-like, powder-like, granular, and liquid forms are particularly preferable. Specifically, supplements, packaged beverages filled in PET bottles, cans, bottles, etc., instant beverages for dissolving in water (hot water), milk, fruit juice, green juice, etc., and food additives. Can be exemplified. These are preferable in that they are easy to drink at the time of meals and can enhance the palatability.

The total content of the pepper plant and vitamins or polyphenols (ingredients of the present invention) in the oral composition of the present invention may be appropriately contained within the range in which the effect is exhibited.

Generally, when the oral composition of the present invention is a pharmaceutical product or a supplement, the component of the present invention is preferably contained in an amount of 0.0001 to 100% by weight, preferably 0.1 to 85% by weight. It is more preferable that it is contained, and it is further preferable that it is contained in an amount of 0.5 to 70% by weight.

When the oral composition of the present invention is a packaged beverage, the components of the present invention are preferably contained in an amount of 0.0001 to 15% by weight, preferably 0.001 to 12.5% by weight. It is more preferable that it is contained, and it is further preferable that it is contained in an amount of 0.01 to 10% by weight.

When the oral composition of the present invention is an instant beverage, the components of the present invention are preferably contained in an amount of 0.001 to 80% by weight, preferably 0.005 to 70% by weight. More preferably, it is further preferably contained in an amount of 0.1 to 60% by weight.

In order to exert the effect of the present invention more effectively, the component of the present invention is preferably contained in an amount of 80% or more, more preferably 90% or more of the entire oral composition of the present invention. It is more preferably 95% or more, and particularly preferably 100%.

The intake amount of the oral composition of the present invention is not particularly limited, but from the viewpoint of exerting the effect of the present invention more remarkably, when the pepper plant is a dried product or a dried crushed product, an adult (65 kg) per day. The intake of the component of the present invention is preferably 100 mg / day or more, more preferably 200 mg / day or more, and 400 mg / day or more. More preferred. The upper limit thereof is not particularly limited, but is, for example, 5000 mg / day, preferably 4000 mg / day. When the pepper plant is juice or extract, it is preferable to use an amount converted based on the weight ratio with the dried product.

The oral composition of the present invention can be contained in one container or divided into a plurality of containers, for example, 2 to 3 so that the daily intake becomes the above-mentioned intake.

The compounding weight ratio of pepper plants and vitamins or polyphenols is preferably in the range of 0.5: 1 to 70: 1, more preferably in the range of 0.75: 1 to 60: 1. The range of: 1 to 60: 1 is more preferable, and the range of 1: 1 to 50: 1 is particularly preferable. When the blending ratio of pepper plants and vitamins or polyphenols is within the above range, the effects of the present invention can be more effectively exhibited.

The oral composition of the present invention can be produced by a known method by adding components other than the components of the present invention that are acceptable for oral use, if necessary.

The pH when the oral composition of the present invention is dissolved in water is not particularly limited, but is preferably in the range of pH 2.0 to 7.0, more preferably pH 3.0 to 6.5, and pH 4.5 to 6. Most preferably.

Hereinafter, the present invention will be described based on examples.
[Example 1]
(material)
As the pepper plant, an ethanol extract obtained by extracting dried products of water pepper buds, long pepper fruits, chili pepper fruits, green pepper fruits, and black pepper fruits with 60% ethanol was used. Each extract was suspended in 10% DMSO, vortexed for 1 hour, centrifuged, and the supernatant was collected. The obtained supernatant was serially diluted to a predetermined concentration so as to have a final concentration of 1% DMSO, and used as a sample solution (pepper solution) in the test.

Examples of vitamins or polyphenols include vitamin B1 hydrochloride, vitamin B2, vitamin B6 (pyridoxine hydrochloride), vitamin B12, nicotinic acid (niacin), sodium D-pantothenate, biotin, folic acid, and vitamin C (L (+)-). Ascorbate sodium), vitamin A, vitamin D3, vitamin E (DL-α tocopherol), vitamin K1, gallic acid, chlorogenic acid, and catechin were all commercially available reagents. After dissolving each reagent in 10% DMSO or 100% DMSO, it was diluted to a predetermined concentration to a final concentration of 1% DMSO and used as a sample solution (vitamins or polyphenol solution) in the test.

(test)
Using ACE Kit-WST (manufactured by Dojin Chemical Laboratory Co., Ltd.), 3-Hydroxybutyric acid (3HB) cut out from 3-Hydroxybutyryl-Gly-Gly-Gly (3HB-GGG) is detected by an enzymatic method. Therefore, inhibition of ACE activity was sought.

10 μL of the sample solution was added to each well. As the sample solution, the solution according to the example of the present invention (5 μL of pepper solution + 5 μL of vitamins or polyphenol solution), the solution of Comparative Example 1 (5 μL of pepper solution + 1% DMSO 5 μL), and the solution of Comparative Example 2 (vitamins or A polyphenol solution (5 μL + 1% DMSO 5 μL) was used. As a blank and a test control, 10 μL of 1% DMSO was used.

Substrate Buffer was added to all wells in an amount of 10 μL each. 10 μL of 1% DMSO was added only to the blank wells, and 10 μL of Enzyme Working Solution was added to the wells of Examples, Comparative Examples 1 and 2, and the test control. Incubated at 37 ± 1 ° C. for 60 minutes. 100 μL of Indicator Working Solution was added to all wells, and the mixture was allowed to stand at room temperature for 10 minutes. The absorbance at 450 nm was measured with an optical analyzer. The ACE inhibitory activity (inhibition rate%) was determined from the absorbance. As the sample blank, 10 μL of the sample solution was added with 120 μL of pure water.

ACE inhibitory activity (inhibition rate%)
= [1- (A _sample −A _{sample blank} ) / (A _{test control} −A _blank )] × 100

test control: Color development without inhibitors (ingredients of the present invention)
blank: Reagent color development
sample blank: Color development of the sample

These results are shown in FIGS. 1 to 5. In the figure, LP indicates long pepper, GP indicates green pepper, WP indicates water pepper, CP indicates chili pepper, and BP indicates black pepper. The numerical value described after the component name indicates the concentration (μg / mL).

As shown in FIGS. 1 to 5, it was revealed that the combination of the plant pepper of the present invention with vitamins or polyphenols enhances the inhibition of ACE activity. In particular, it was found that vitamins and polyphenols can enhance the ACE activity inhibitory action by combining with pepper plants even when the blending amount is smaller than that of pepper plants.

[Example 2] (Production of tablets)
Three tablets (3600 mg) composed of the following ingredients were produced.

Water pepper bud dry powder 20% by weight
Vitamin B1 hydrochloride 1% by weight
Folic acid 1% by weight
Gallic acid 1% by weight
Cellulose 2% by weight
Silicon dioxide 2% by weight
Calcium stearate 1% by weight
Shellac 1% by weight
Reduced maltose balance

The above tablets should be taken once a day or in 2 to 3 divided doses with water.

[Example 3] (Production of tablets)
Three tablets (3600 mg) composed of the following ingredients were produced.

Water pepper bud extract 6% by weight
Vitamin B6 2% by weight
Catechin 1% by weight
Cellulose 2% by weight
Silicon dioxide 2% by weight
Calcium stearate 1% by weight
Shellac 1% by weight
Reduced maltose balance

The above tablets should be taken once a day or in 2 to 3 divided doses with water.

[Example 4] (Manufacturing of capsules)
The following mixture was encapsulated in soft capsules to produce capsules.

Long pepper fruit dry powder 100mg
Niacin acid 10 mg
Chlorogenic acid 5 mg
Leucine 2.5 mg
Tyrosine 2.5 mg
Valine 2.5 mg
Iron chloride 1 mg
Maltose 78.5 mg

The above capsules are taken in 4 capsules 2 to 4 times a day with water.

[Example 5] (Production of capsule)
The following mixture was encapsulated in soft capsules to produce capsules.

Long pepper fruit extract 50mg
Niacin acid 10 mg
Chlorogenic acid 5 mg
Leucine 2.5 mg
Tyrosine 2.5 mg
Valine 2.5 mg
Iron chloride 1 mg
Maltose 128.5 mg

The above capsules are taken in 2 capsules once or twice a day with water.

[Example 6] (Production of granules)
The following components were mixed to prepare granules (3000 mg) by a conventional method.

Green pepper fruit extract 500mg
Reduced isomaltulose 500 mg
Silicon dioxide 200mg
Sucrose fatty acid ester 50 mg
Inositol 25 mg
Vitamin A 10 mg
Zinc gluconate 10 mg
Magnesium chloride 5 mg
Threonine 5 mg
Tryptophan 2.5 mg
Starch residue

[Example 7] (Production of instant powder)
The following ingredients were mixed to produce an instant powder (10 g) by a conventional method.

Soy milk powder 5g
Chili pepper fruit extract 0.25g
Citrulline 1g
Vitamin E 0.02g
Vitamin K1 0.02g
Glutamine 0.01g
Zinc gluconate 0.001 g
Iron chloride 0.001g
Reduced palatinose 0.1g
Calcium stearate 2g
Indigestible dextrin balance

[Example 8] (Production of liquid preparation)
A liquid preparation (500 mL) consisting of the following components was produced.

Black pepper fruit extract 10 mg
Trehalose 500mg
Calcium chloride 50 mg
Valine 25 mg
Leucine 25 mg
Isoleucine 25 mg
Phenylalanine 10 mg
Proline 10 mg
Sodium D-pantothenate 300 mg
Vitamin K1 150mg
Vitamin C 50 mg
Remaining water

Since the oral composition of the present invention has an effect of preventing and / or ameliorating hypertension and can be used as a so-called health food or the like, the industrial usefulness of the present invention is high.

Claims (9)

An oral composition comprising a pepper plant and vitamins or polyphenols. The oral composition according to claim 1, wherein the pepper plant is at least one selected from water pepper, long pepper, green pepper, chili pepper, and black pepper. The oral composition according to claim 2, wherein the pepper plant is at least one selected from water pepper, long pepper, green pepper, and chili pepper. At least vitamins are selected from vitamin B1 hydrochloride, vitamin B2, vitamin B6, vitamin B12, nicotinic acid, sodium D-pantothenate, biotin, folic acid, vitamin C, vitamin A, vitamin D3, vitamin E, and vitamin K. The oral composition according to any one of claims 1 to 3, wherein the oral composition is one kind. The oral composition according to any one of claims 1 to 4, wherein the vitamins are at least one fat-soluble vitamin selected from vitamin A, vitamin D3, vitamin E, and vitamin K. The oral composition according to any one of claims 1 to 5, wherein the polyphenol is at least one selected from gallic acid, chlorogenic acid, and catechin. The oral composition according to any one of claims 1 to 6, wherein the oral composition is in the form of a tablet, a capsule, a powder, a granule, or a liquid. The oral composition according to any one of claims 1 to 7, which is a composition for preventing and improving hypertension used for preventing and / or ameliorating hypertension. The oral composition according to any one of claims 1 to 8, characterized in that it is a functional food.

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