JP2021141837A - Beer taste beverage and method for producing the same - Google Patents
Beer taste beverage and method for producing the same Download PDFInfo
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- JP2021141837A JP2021141837A JP2020042201A JP2020042201A JP2021141837A JP 2021141837 A JP2021141837 A JP 2021141837A JP 2020042201 A JP2020042201 A JP 2020042201A JP 2020042201 A JP2020042201 A JP 2020042201A JP 2021141837 A JP2021141837 A JP 2021141837A
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- purine
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- white clay
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- 235000013361 beverage Nutrition 0.000 title claims abstract description 67
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 51
- 235000013405 beer Nutrition 0.000 title abstract description 7
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 claims abstract description 102
- 239000004927 clay Substances 0.000 claims abstract description 47
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- 239000002994 raw material Substances 0.000 description 22
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- LRFVTYWOQMYALW-UHFFFAOYSA-N 9H-xanthine Chemical compound O=C1NC(=O)NC2=C1NC=N2 LRFVTYWOQMYALW-UHFFFAOYSA-N 0.000 description 14
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- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 description 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 2
- 239000004382 Amylase Substances 0.000 description 2
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- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 description 2
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- SHZIWNPUGXLXDT-UHFFFAOYSA-N ethyl hexanoate Chemical compound CCCCCC(=O)OCC SHZIWNPUGXLXDT-UHFFFAOYSA-N 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 235000003599 food sweetener Nutrition 0.000 description 2
- FDGQSTZJBFJUBT-UHFFFAOYSA-N hypoxanthine Chemical compound O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 description 2
- PHTQWCKDNZKARW-UHFFFAOYSA-N isoamylol Chemical compound CC(C)CCO PHTQWCKDNZKARW-UHFFFAOYSA-N 0.000 description 2
- ZXEKIIBDNHEJCQ-UHFFFAOYSA-N isobutanol Chemical compound CC(C)CO ZXEKIIBDNHEJCQ-UHFFFAOYSA-N 0.000 description 2
- 239000001630 malic acid Substances 0.000 description 2
- 235000011090 malic acid Nutrition 0.000 description 2
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- 229910052757 nitrogen Inorganic materials 0.000 description 2
- JYVLIDXNZAXMDK-UHFFFAOYSA-N pentan-2-ol Chemical compound CCCC(C)O JYVLIDXNZAXMDK-UHFFFAOYSA-N 0.000 description 2
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- 239000002126 C01EB10 - Adenosine Substances 0.000 description 1
- UDMBCSSLTHHNCD-UHFFFAOYSA-N Coenzym Q(11) Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(O)=O)C(O)C1O UDMBCSSLTHHNCD-UHFFFAOYSA-N 0.000 description 1
- MIKUYHXYGGJMLM-GIMIYPNGSA-N Crotonoside Natural products C1=NC2=C(N)NC(=O)N=C2N1[C@H]1O[C@@H](CO)[C@H](O)[C@@H]1O MIKUYHXYGGJMLM-GIMIYPNGSA-N 0.000 description 1
- NYHBQMYGNKIUIF-UHFFFAOYSA-N D-guanosine Natural products C1=2NC(N)=NC(=O)C=2N=CN1C1OC(CO)C(O)C1O NYHBQMYGNKIUIF-UHFFFAOYSA-N 0.000 description 1
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 description 1
- 239000004278 EU approved seasoning Substances 0.000 description 1
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- 201000005569 Gout Diseases 0.000 description 1
- UGQMRVRMYYASKQ-UHFFFAOYSA-N Hypoxanthine nucleoside Natural products OC1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 UGQMRVRMYYASKQ-UHFFFAOYSA-N 0.000 description 1
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- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 description 1
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- UDMBCSSLTHHNCD-KQYNXXCUSA-N adenosine 5'-monophosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O UDMBCSSLTHHNCD-KQYNXXCUSA-N 0.000 description 1
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- KDYFGRWQOYBRFD-NUQCWPJISA-N butanedioic acid Chemical compound O[14C](=O)CC[14C](O)=O KDYFGRWQOYBRFD-NUQCWPJISA-N 0.000 description 1
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Landscapes
- Distillation Of Fermentation Liquor, Processing Of Alcohols, Vinegar And Beer (AREA)
Abstract
Description
本発明は、ビールテイスト飲料およびその製造方法に関する。 The present invention relates to a beer-taste beverage and a method for producing the same.
ビールテイスト飲料には原料の麦芽に由来するプリン体が含まれている。近年痛風予防を目的とした健康志向の高まりから、プリン体含有量の低いビールテイスト飲料が望まれており、活性炭や白土を用いたプリン体吸着剤によりプリン体含有量の低いビールテイスト飲料を製造する試みがなされている(特許文献1、2)。 Beer-taste beverages contain purines derived from the raw material malt. In recent years, beer-taste beverages with a low purine content have been desired due to increasing health consciousness for the purpose of preventing gout, and beer-taste beverages with a low purine content are produced by purine adsorbents using activated carbon or white clay. Attempts have been made (Patent Documents 1 and 2).
しかしながら、特許文献1、2の方法ではプリン体を低減するために大量の吸着剤を用いる必要があるため、より効率的な方法が求められる。 However, in the methods of Patent Documents 1 and 2, it is necessary to use a large amount of adsorbent in order to reduce purines, so that a more efficient method is required.
本発明は、プリン体を効率的に低減できるビールテイスト飲料の製造方法、及び当該製造方法によりプリン体が低減されたビールテイスト飲料を提供することに関する。 The present invention relates to a method for producing a beer-taste beverage in which purines can be efficiently reduced, and to provide a beer-taste beverage in which purines are reduced by the production method.
本発明は、下記[1]〜[2]に関する。
[1]ヌクレオシダーゼ処理を行う酵素処理工程を有するビールテイスト飲料の製造方法であって、前記酵素処理工程と同時か又はそれ以降に白土を用いたプリン体除去工程を行う、ビールテイスト飲料の製造方法。
[2][1]に記載の製造方法により得られるビールテイスト飲料。
The present invention relates to the following [1] to [2].
[1] A method for producing a beer-taste beverage having an enzyme-treated step of performing nucleosidase treatment, wherein a purine-removing step using white clay is performed at the same time as or after the enzyme-treated step. Method.
[2] A beer-taste beverage obtained by the production method according to [1].
本発明によれば、プリン体を効率的に低減できるビールテイスト飲料の製造方法、及び当該製造方法によりプリン体が低減されたビールテイスト飲料を提供することができる。 According to the present invention, it is possible to provide a method for producing a beer-taste beverage in which purines can be efficiently reduced, and a beer-taste beverage in which purines are reduced by the production method.
本発明の発明者らは、上記課題について鋭意検討した結果、酵素処理工程と白土を用いたプリン体除去工程を行うことにより効率的にプリン体を低減できることを新たに見出した。かかるメカニズムについては定かではないが、ヌクレオシダーゼで処理した後の酵母のグアニンのプリン体代謝先としてキサンチンが知られており、キサンチンが残ってしまうためヌクレオシダーゼ処理だけでは十分にプリン体が低減しない。白土はキサンチンを効果的に吸着除去できるため、酵素処理工程と白土を用いたプリン体除去工程を行うことによりキサンチンを含めたプリン体を大きく除去できるものと推定される。 As a result of diligent studies on the above problems, the inventors of the present invention have newly found that purines can be efficiently reduced by performing an enzyme treatment step and a purine removal step using white clay. Although the mechanism is not clear, xanthine is known as a purine metabolism destination of yeast guanine after treatment with nucleosidase, and since xanthine remains, purine nucleosidase treatment alone does not sufficiently reduce purines. .. Since white clay can effectively adsorb and remove xanthine, it is presumed that purines containing xanthine can be largely removed by performing an enzyme treatment step and a purine body removing step using white clay.
本発明の製造方法は、酵素処理工程及びプリン体除去工程を有するビールテイスト飲料の製造方法に関する。 The production method of the present invention relates to a method for producing a beer-taste beverage having an enzyme treatment step and a purine body removal step.
本明細書においてプリン体とは、プリン骨格を持つ化合物の総称を指す。アデニン、グアニン、キサンチン、ヒポキサンチンのプリン体塩基に加えて、アデノシン、グアノシン等のようなプリンヌクレオシド、アデニル酸、グアニル酸などのようなプリンヌクレオチドもプリン体に含まれる。本明細書において、プリン体の含有量は、過塩素酸による加水分解後に液体クロマトグラフィー質量分析装置(LC-MS/MS)で測定する(「酒類中のプリン体の微量分析のご案内」日本食品分析センターhttp://www.jfrl.or.jp/item/nutrition/post-31.html)。 As used herein, the term "purine compound" refers to a general term for compounds having a purine skeleton. In addition to the purine bases of adenin, guanine, xanthine, and hypoxanthine, purine nucleotides such as purine nucleosides such as adenosine and guanosine, and purine nucleotides such as adenylic acid and guanylic acid are also included in the purine body. In the present specification, the content of purines is measured by a liquid chromatography-mass spectrometer (LC-MS / MS) after hydrolysis with perchloric acid ("Guide to trace analysis of purines in alcoholic beverages" Japan. Food Analysis Center http://www.jfrl.or.jp/item/nutrition/post-31.html).
本明細書において「ビールテイスト飲料」とは、ビール様の風味をもつ炭酸飲料をいう。つまり、本明細書のビールテイスト飲料は、特に断わりがない場合、ビール風味の炭酸飲料を全て包含し、アルコールを含有するビールテイスト飲料及びノンアルコールビールテイスト飲料が挙げられる。このときのアルコールとはエタノールを指し、脂肪族アルコールは含まれない。アルコールを含有するビールテイスト飲料におけるエタノール含量としては、容量比で1%〜10%が好ましいが、特に限定されるものではない。さらに、ビールテイスト飲料に含まれるアルコール分の由来としては、醗酵、非醗酵に限定されるものではない。また、ノンアルコールビールテイスト飲料とは、アルコール度数が1%未満のビールテイスト飲料であり、好ましくは、アルコールを実質的に含まない。ここで、アルコールを実質的に含まない態様の飲料は、検出できない程度の極微量のアルコールを含有する飲料を除くものではない。アルコール度数が四捨五入により0.0%となる飲料、中でも、アルコール度数が四捨五入により0.00%となる飲料は、ノンアルコールビールテイスト飲料に包含される。 As used herein, the term "beer-taste beverage" refers to a carbonated beverage having a beer-like flavor. That is, unless otherwise specified, the beer-taste beverages described in the present specification include all beer-flavored carbonated beverages, and include beer-taste beverages containing alcohol and non-alcoholic beer-taste beverages. Alcohol at this time refers to ethanol and does not include aliphatic alcohol. The ethanol content in the beer-taste beverage containing alcohol is preferably 1% to 10% by volume, but is not particularly limited. Furthermore, the origin of the alcohol content contained in the beer-taste beverage is not limited to fermented and non-fermented. The non-alcoholic beer-taste beverage is a beer-taste beverage having an alcohol content of less than 1%, and preferably contains substantially no alcohol. Here, the beverages in a mode that does not substantially contain alcohol do not exclude beverages that contain an undetectable trace amount of alcohol. Beverages whose alcohol content is rounded to 0.0%, especially those whose alcohol content is rounded to 0.00%, are included in non-alcoholic beer-taste beverages.
本明細書においてアルコール度数は、飲料中のアルコール分の含有量(v/v%)を意味し、公知のいずれの方法によっても測定することができるが、例えば、振動式密度計によって測定することができる。具体的には、飲料から濾過又は超音波によって炭酸ガスを抜いた試料を調製し、そして、その試料を直火蒸留し、得られた留液の15℃における密度を測定し、国税庁所定分析法(平19国税庁訓令第6号、平成19年6月22日改訂)の付表である「第2表 アルコール分と密度(15℃)及び比重(15/15℃)換算表」を用いて換算して求めることができる。アルコール度が1.0%未満の低濃度の場合は、市販のアルコール測定装置や、ガスクロマトグラフィーを用いても良い。 In the present specification, the alcohol content means the content of alcohol in a beverage (v / v%) and can be measured by any known method, but for example, it is measured by a vibration densitometer. Can be done. Specifically, a sample from which carbon dioxide gas has been removed by filtration or ultrasonic waves from the beverage is prepared, and the sample is distilled by direct fire to measure the density of the obtained distillate at 15 ° C. Converted using "Table 2 Alcohol content and density (15 ° C) and specific gravity (15/15 ° C) conversion table" which is an attached table of (Heisei 19 National Tax Agency Instruction No. 6, revised on June 22, 2007). Can be obtained. When the alcohol content is low, less than 1.0%, a commercially available alcohol measuring device or gas chromatography may be used.
本発明の製造方法に係る酵素処理工程は、ヌクレオシダーゼ処理を行う工程である。本発明の製造方法に係る酵素処理工程は、ビールテイスト飲料の製造工程における任意の工程において行うことができるが、酵素処理で得られたプリン塩基を酵母に資化させる観点から、発酵終了前に行うことが好ましい。酵素処理条件は例えば、糖化工程にて酵素を添加し糖化温度条件下で処理しても良いし、発酵工程にて酵素を添加し発酵温度条件下で処理しても良い。また、これらの条件を組み合わせて処理しても良い。 The enzyme treatment step according to the production method of the present invention is a step of performing nucleosidase treatment. The enzyme treatment step according to the production method of the present invention can be carried out in any step in the production step of the beer-taste beverage, but from the viewpoint of assimilating the purine base obtained by the enzyme treatment into yeast, before the completion of fermentation. It is preferable to do so. As the enzyme treatment conditions, for example, the enzyme may be added in the saccharification step and treated under the saccharification temperature condition, or the enzyme may be added in the fermentation step and treated under the fermentation temperature condition. Moreover, you may process by combining these conditions.
本発明の製造方法に係るプリン体除去工程は、吸着剤として白土を用いてプリン体を除去する工程である。本発明の製造方法に係るプリン体除去工程は、ビールテイスト飲料の製造工程における任意の工程において行うことができるが、酵素処理工程と同時か又はそれ以降に行うことが好ましく、複数回行ってもよい。本発明の製造方法に係るプリン体除去工程は、中味液に白土を添加して行う。本発明の製造方法に係るプリン体除去工程は、プリン体除去効率を良くする観点から、分散手段により白土を中味液中に分散させて行うことが好ましく、さらに香気成分の減少を抑制する観点から、大気圧より高い圧力下において行うことが好ましい。分散の態様として、添加した白土の一部が沈降していても構わないが、添加した白土のすべてが分散していることが好ましい。本明細書において、中味液とは、プリン体除去の対象となる液体を指す。中味液は、どの工程においてプリン体除去工程を行うかにより左右され、例えば、糖化液、麦汁、もろみ、ビールテイスト飲料、その他ビールテイスト飲料を構成する原料の懸濁液などの中味液が挙げられる。 The purine body removing step according to the production method of the present invention is a step of removing the purine body using white clay as an adsorbent. The purine body removing step according to the manufacturing method of the present invention can be carried out in any step in the manufacturing step of the beer-taste beverage, but it is preferably carried out at the same time as or after the enzyme treatment step, and even if it is carried out a plurality of times. good. The purine body removing step according to the production method of the present invention is carried out by adding white clay to the content liquid. The purine body removing step according to the production method of the present invention is preferably carried out by dispersing white clay in the content liquid by a dispersing means from the viewpoint of improving the purine body removing efficiency, and further from the viewpoint of suppressing the decrease of the aroma component. , It is preferable to carry out under a pressure higher than the atmospheric pressure. As a mode of dispersion, a part of the added white clay may be settled, but it is preferable that all of the added white clay is dispersed. In the present specification, the content liquid refers to a liquid to be removed from purines. The content liquid depends on the process in which the purine removal step is performed. For example, the content liquid includes a saccharified liquid, wort, mash, beer-taste beverage, and a suspension of raw materials constituting the beer-taste beverage. Beer.
本発明の製造方法に係る白土としては、モンモリロナイトを主要成分とする粘土である酸性白土や、酸性白土を酸処理した活性白土が挙げられ、好ましくは活性白土である。 Examples of the clay according to the production method of the present invention include acid clay, which is a clay containing montmorillonite as a main component, and activated clay obtained by acid-treating acid clay, and are preferably activated clay.
本発明の製造方法に係る中味液への白土の添加量は、中味液のプリン体量に応じて調整すればよく特に限定されるものではないが、プリン体除去効率の観点から、好ましくは1ppm以上であり、より好ましくは5ppm以上であり、更に好ましくは10ppm以上であり、また、コストや白土の分離など生産性の観点から、好ましくは20000ppm以下であり、より好ましくは15000ppm以下であり、更に好ましくは10000ppm以下であり、これらいずれの組み合わせによる範囲としてもよい。 The amount of white clay added to the content liquid according to the production method of the present invention may be adjusted according to the amount of purine in the content liquid and is not particularly limited, but is preferably 1 ppm from the viewpoint of purine removal efficiency. It is more preferably 5 ppm or more, further preferably 10 ppm or more, and from the viewpoint of productivity such as cost and separation of white clay, it is preferably 20000 ppm or less, more preferably 15000 ppm or less, and further. It is preferably 10000 ppm or less, and may be in the range of any combination of these.
本発明の製造方法に係るプリン体除去工程における圧力は、大気圧より高い圧力下であれば特に限定されないが、大気圧に加えてさらに以下の加圧条件下で行うことが好ましい。当該加圧条件は、香気成分保持の観点から、好ましくは0.5kPa以上であり、より好ましくは1.0kPa以上であり、さらに好ましくは1.5kPa以上であり、また、発酵中の酵母への影響や次工程への液送のしやすさの観点から、好ましくは200kPa以下であり、より好ましくは150kPa以下であり、さらに好ましくは100kPa以下であり、これらいずれの組み合わせによる範囲としてもよい。 The pressure in the purine body removing step according to the production method of the present invention is not particularly limited as long as it is under a pressure higher than the atmospheric pressure, but it is preferably performed under the following pressure conditions in addition to the atmospheric pressure. From the viewpoint of retaining the aroma component, the pressurizing condition is preferably 0.5 kPa or more, more preferably 1.0 kPa or more, still more preferably 1.5 kPa or more, and to yeast during fermentation. From the viewpoint of influence and ease of liquid feeding to the next step, it is preferably 200 kPa or less, more preferably 150 kPa or less, still more preferably 100 kPa or less, and the range may be any combination of these.
加圧手段としては、例えば、タンクの圧力弁をあらかじめ設定しておき発酵で生成する炭酸ガスを利用する方法、外部から炭酸ガスなどのガスを注入して加圧する方法などが挙げられる。 Examples of the pressurizing means include a method in which a pressure valve of the tank is set in advance and carbon dioxide gas generated by fermentation is used, and a method in which a gas such as carbon dioxide gas is injected from the outside to pressurize.
本発明の製造方法に係る分散手段としては、攪拌、バブリング、工程内での循環接触などが挙げられる。例えば、撹拌機を備え付けた容器内で撹拌しながら対流を起こす方法や、炭酸ガスなどのガスを容器に注入して対流を起こす方法、また、工程内での循環接触とは、ループする配管を備えた容器中に中味液と白土を加え、配管内を循環させることで分散状態を維持する方法を指し、例えば、貯酒タンクにループする配管を備え付け、白土と一緒に貯酒を循環させる方法などが挙げられる。また、分散手段として、発酵により発生する対流を利用して分散させてもよい。好ましくはバブリングもしくは発酵による対流利用の態様である。 Dispersing means according to the production method of the present invention include stirring, bubbling, circulation contact in the process, and the like. For example, a method of causing convection while stirring in a container equipped with a stirrer, a method of injecting a gas such as carbon dioxide into the container to cause convection, and a circulation contact in a process include a looping pipe. It refers to a method of maintaining a dispersed state by adding the content liquid and white clay to the prepared container and circulating it in the pipe. Can be mentioned. Further, as the dispersion means, the convection generated by fermentation may be used for dispersion. It is preferably a mode of convection utilization by bubbling or fermentation.
本発明の製造方法に係る中味液における白土の分散率は、プリン体除去効率の観点から、好ましくは10%以上であり、より好ましくは15%以上であり、更に好ましくは20%以上であり、更に好ましくは30%以上であり、更に好ましくは40%以上であり、更に好ましくは50%以上であり、更に好ましくは60%以上であり、更に好ましくは70%以上であり、更に好ましくは80%以上であり、更に好ましくは90%以上であり、上限は特に限定されないが、例えば100%以下とすることができ、これらいずれの組み合わせによる範囲としてもよい。本明細書において中味液における白土の分散率は、中味液の液面の高さの半分の位置における吸着剤の分散の程度を示すものであり、後述の実施例に記載の方法により測定して算出する。 From the viewpoint of purine removal efficiency, the dispersion ratio of white clay in the content liquid according to the production method of the present invention is preferably 10% or more, more preferably 15% or more, still more preferably 20% or more. It is more preferably 30% or more, still more preferably 40% or more, still more preferably 50% or more, still more preferably 60% or more, still more preferably 70% or more, still more preferably 80%. The above is more preferably 90% or more, and the upper limit is not particularly limited, but can be, for example, 100% or less, and may be in the range of any combination of these. In the present specification, the dispersion rate of white clay in the content liquid indicates the degree of dispersion of the adsorbent at a position half the height of the liquid surface of the content liquid, and is measured by the method described in Examples described later. calculate.
本発明の製造方法に係るプリン体除去工程における分散処理時間は、プリン体除去効率の観点から、好ましくは15分以上であり、より好ましくは1時間以上であり、更に好ましくは3時間以上であり、また、生産性の観点から、好ましくは10時間以下であり、より好ましくは7時間以下であり、更に好ましくは5時間以下であり、これらいずれの組み合わせによる範囲としてもよい。 The dispersion treatment time in the purine body removing step according to the production method of the present invention is preferably 15 minutes or more, more preferably 1 hour or more, still more preferably 3 hours or more, from the viewpoint of purine body removal efficiency. Further, from the viewpoint of productivity, it is preferably 10 hours or less, more preferably 7 hours or less, still more preferably 5 hours or less, and the range may be any combination of these.
本発明の製造方法の態様としては、アルコールを含有するビールテイスト飲料及びノンアルコールビールテイスト飲料の製造方法が挙げられるが、プリン体除去工程を有する以外は一般的なビールテイスト飲料の製造方法と同様である。以下に、ビールテイスト飲料の製造態様を例示する。ビールテイスト飲料の製造態様としては麦芽を原料として使用するものとしないものとがあり、以下のように製造することができる。 Examples of the production method of the present invention include a method for producing a beer-taste beverage containing alcohol and a non-alcoholic beer-taste beverage, which is the same as a general method for producing a beer-taste beverage except that it has a purine body removing step. Is. The production mode of the beer-taste beverage will be illustrated below. There are two types of beer-taste beverages, one in which malt is used as a raw material and the other in which malt is not used as a raw material, and the beer-taste beverage can be produced as follows.
麦芽を原料として使用して製造されるアルコールを含有するビールテイスト飲料は、まず、麦芽等の麦の他、必要に応じて他の穀物、でんぷん、糖類、苦味料、又は着色料などの原料及び水を含む混合物に、必要に応じてアミラーゼなどの酵素を添加し、糊化、糖化を行なわせ、ろ過し、糖化液とする。必要に応じてホップや苦味料などを糖化液に加えて煮沸し、清澄タンクにて凝固タンパク質などの固形分を取り除く。この糖化液の代替として、麦芽エキスに温水を加えたものにホップを加えて煮沸してもよい。ホップは煮沸開始から煮沸終了前のどの段階で混合してもよい。糖化工程、煮沸工程、固形分除去工程などにおける条件は、知られている条件を用いればよい。醗酵・貯酒工程などにおける条件は、知られている条件を用いればよい。得られた醗酵液を濾過し、得られた濾過液に炭酸ガスを加える。その後、容器に充填し殺菌工程を経て目的のビールテイスト飲料を得る。なお、アルコール成分として、さらに、穀物に由来するスピリッツを添加してもよい。スピリッツとは、麦、米、そば、とうもろこし等の穀物を原料として、酵母を用いて発酵させた後、更に蒸留して得られる酒類を意味する。スピリッツの原材料である穀物としては麦が好ましい。前記各工程において酵素処理工程は充填までのどの工程で行ってもよいが、プリン体除去効率の観点から、発酵工程において行うことが好ましい。また、前記各工程においてプリン体除去工程は充填までのどの工程で行ってもよいが、プリン体除去効率の観点から、発酵・貯酒もしくは濾過の工程において行うことが好ましい。 Alcohol-containing beer-taste beverages produced using malt as a raw material include, first of all, in addition to wheat such as malt, raw materials such as other grains, starch, saccharides, bitterness, or coloring agents as needed. If necessary, an enzyme such as amylase is added to the mixture containing water to gelatinize and saccharify, and the mixture is filtered to obtain a saccharified solution. If necessary, add hops and bitterness to the saccharified solution and boil, and remove solids such as coagulated protein in a clarification tank. As an alternative to this saccharified solution, hops may be added to a malt extract mixed with warm water and boiled. Hops may be mixed at any stage from the start of boiling to the end of boiling. As the conditions in the saccharification step, the boiling step, the solid content removing step and the like, known conditions may be used. As the conditions in the fermentation / liquor storage process and the like, known conditions may be used. The obtained fermentation broth is filtered, and carbon dioxide gas is added to the obtained filtrate. After that, it is filled in a container and sterilized to obtain a desired beer-taste beverage. In addition, as an alcohol component, spirits derived from grains may be further added. Spirits means alcoholic beverages obtained by fermenting grains such as wheat, rice, buckwheat, and corn with yeast and then further distilling them. Wheat is preferred as the grain that is the raw material for spirits. In each of the above steps, the enzyme treatment step may be carried out in any step up to filling, but from the viewpoint of purine removal efficiency, it is preferably carried out in the fermentation step. Further, in each of the above steps, the purine body removing step may be carried out in any step up to filling, but from the viewpoint of purine body removing efficiency, it is preferable to carry out in the fermentation / liquor storage or filtration step.
麦芽を原料として使用せずに製造されるアルコールを含有するビールテイスト飲料は、炭素源を含有する液糖、麦又は麦芽以外のアミノ酸含有材料としての窒素源、ホップ、色素等を、温水と共に混合し、液糖溶液とする。該液糖溶液は、煮沸する。原料としてホップを用いる場合、ホップは煮沸開始前ではなく、煮沸中に、該液糖溶液に混合してもよい。この糖化液の代替として、麦芽以外の原料を用いたエキスに温水を加えたものにホップを加えて煮沸してもよい。ホップは煮沸開始から煮沸終了前のどの段階で混合してもよい。醗酵・貯酒工程などにおける条件は、知られている条件を用いればよい。得られた醗酵液を濾過し、得られた濾過液に炭酸ガスを加える。その後、容器に充填し殺菌工程を経て目的のビールテイスト飲料を得る。なお、アルコール成分として、さらに、穀物に由来するスピリッツを添加してもよい。前記各工程において酵素処理工程は充填までのどの工程で行ってもよいが、プリン体除去効率の観点から、発酵工程において行うことが好ましい。また、前記各工程においてプリン体除去工程は充填までのどの工程で行ってもよいが、プリン体除去効率の観点から、発酵・貯酒もしくは濾過の工程において行うことが好ましい。 Alcohol-containing beer-taste beverages produced without using malt as a raw material are a mixture of liquid sugar containing a carbon source, nitrogen source as an amino acid-containing material other than wheat or malt, hops, pigments, etc. together with warm water. Then, use a liquid sugar solution. The liquid sugar solution is boiled. When hops are used as a raw material, the hops may be mixed with the liquid sugar solution during boiling, not before the start of boiling. As an alternative to this saccharified solution, hops may be added to an extract using a raw material other than malt with warm water and boiled. Hops may be mixed at any stage from the start of boiling to the end of boiling. As the conditions in the fermentation / liquor storage process and the like, known conditions may be used. The obtained fermentation broth is filtered, and carbon dioxide gas is added to the obtained filtrate. After that, it is filled in a container and sterilized to obtain a desired beer-taste beverage. In addition, as an alcohol component, spirits derived from grains may be further added. In each of the above steps, the enzyme treatment step may be carried out in any step up to filling, but from the viewpoint of purine removal efficiency, it is preferably carried out in the fermentation step. Further, in each of the above steps, the purine body removing step may be carried out in any step up to filling, but from the viewpoint of purine body removing efficiency, it is preferable to carry out in the fermentation / liquor storage or filtration step.
非醗酵かつアルコールを含有するビールテイスト飲料は、麦芽を使用する、しないに限らず、原料用アルコールなどを加えることにより最終製品のアルコール分を調整したものでもよい。原料用アルコールの添加は、糖化工程から充填工程までのどの工程で行ってもよい。なお、アルコール成分として、さらに、穀物に由来するスピリッツを添加してもよい。前記各工程において酵素処理工程は充填までのどの工程で行ってもよいが、プリン体除去効率の観点から、原料調合後の工程において行うことが好ましい。前記各工程においてプリン体除去工程は充填までのどの工程で行ってもよいが、プリン体除去効率の観点から、煮沸終了以降の工程において行うことが好ましい。 The non-fermented and alcohol-containing beer-taste beverage may or may not use malt, and the alcohol content of the final product may be adjusted by adding alcohol for raw materials. The alcohol for raw materials may be added in any step from the saccharification step to the filling step. In addition, as an alcohol component, spirits derived from grains may be further added. In each of the above steps, the enzyme treatment step may be carried out in any step up to filling, but from the viewpoint of purine removal efficiency, it is preferably carried out in the step after the preparation of the raw material. In each of the above steps, the purine body removing step may be carried out in any step up to filling, but from the viewpoint of purine body removing efficiency, it is preferable to carry out in the steps after the completion of boiling.
麦芽を原料として使用して製造されるノンアルコールビールテイスト飲料は、まず、麦芽等の麦の他、必要に応じて他の穀物、でんぷん、糖類、苦味料、又は着色料などの原料及び水を含む混合物に、必要に応じてアミラーゼなどの酵素を添加し、糊化、糖化を行なわせ、ろ過し、糖化液とする。必要に応じてホップや苦味料などを糖化液に加えて煮沸し、清澄タンクにて凝固タンパク質などの固形分を取り除く。この糖化液の代替として、麦芽エキスに温水を加えたものにホップを加えて煮沸してもよい。ホップは煮沸開始から煮沸終了前のどの段階で混合してもよい。糖化工程、煮沸工程、固形分除去工程などにおける条件は、知られている条件を用いればよい。煮沸後、得られた麦汁を濾過し、得られた濾過液に炭酸ガスを加える。その後、容器に充填し殺菌工程を経て目的のノンアルコールビールテイスト飲料を得る。前記各工程において酵素処理工程は充填までのどの工程で行ってもよいが、プリン体除去効率の観点から、原料調合後の工程において行うことが好ましい。前記各工程においてプリン体除去工程は充填までのどの工程で行ってもよいが、プリン体除去効率の観点から、煮沸終了以降の工程において行うことが好ましい。 For non-alcoholic beer-taste beverages produced using malt as a raw material, first, in addition to wheat such as malt, other grains, starch, saccharides, bitterness, or coloring agents and water are added as needed. If necessary, an enzyme such as amylase is added to the containing mixture to gelatinize and saccharify, and the mixture is filtered to obtain a saccharified solution. If necessary, add hops and bitterness to the saccharified solution and boil, and remove solids such as coagulated protein in a clarification tank. As an alternative to this saccharified solution, hops may be added to a malt extract mixed with warm water and boiled. Hops may be mixed at any stage from the start of boiling to the end of boiling. As the conditions in the saccharification step, the boiling step, the solid content removing step and the like, known conditions may be used. After boiling, the obtained wort is filtered, and carbon dioxide gas is added to the obtained filtrate. Then, it is filled in a container and sterilized to obtain a desired non-alcoholic beer-taste beverage. In each of the above steps, the enzyme treatment step may be carried out in any step up to filling, but from the viewpoint of purine removal efficiency, it is preferably carried out in the step after the preparation of the raw material. In each of the above steps, the purine body removing step may be carried out in any step up to filling, but from the viewpoint of purine body removing efficiency, it is preferable to carry out in the steps after the completion of boiling.
麦芽を原料として使用しないノンアルコールビールテイスト飲料を製造する場合には、まず、炭素源を含有する液糖、麦又は麦芽以外のアミノ酸含有材料としての窒素源、ホップ、色素等を、温水と共に混合し、液糖溶液とする。該液糖溶液は、煮沸する。原料としてホップを用いる場合、ホップは煮沸開始前ではなく、煮沸中に、該液糖溶液に混合してもよい。煮沸後の液糖溶液に対して、炭酸ガスを加える。その後、容器に充填し殺菌工程を経て目的のノンアルコールビールテイスト飲料を得る。前記各工程において酵素処理工程は充填までのどの工程で行ってもよいが、プリン体除去効率の観点から、原料調合後の工程において行うことが好ましい。前記各工程においてプリン体除去工程は充填までのどの工程で行ってもよいが、プリン体除去効率の観点から、煮沸終了以降の工程において行うことが好ましい。 When producing a non-alcoholic beer-taste beverage that does not use malt as a raw material, first, liquid sugar containing a carbon source, nitrogen source as an amino acid-containing material other than wheat or malt, hops, pigments, etc. are mixed with warm water. Then, use a liquid sugar solution. The liquid sugar solution is boiled. When hops are used as a raw material, the hops may be mixed with the liquid sugar solution during boiling, not before the start of boiling. Carbon dioxide gas is added to the liquid sugar solution after boiling. Then, it is filled in a container and sterilized to obtain a desired non-alcoholic beer-taste beverage. In each of the above steps, the enzyme treatment step may be carried out in any step up to filling, but from the viewpoint of purine removal efficiency, it is preferably carried out in the step after the preparation of the raw material. In each of the above steps, the purine body removing step may be carried out in any step up to filling, but from the viewpoint of purine body removing efficiency, it is preferable to carry out in the steps after the completion of boiling.
本発明の製造方法においては、酒感を付与する観点から、脂肪族アルコールを添加してもよい。脂肪族アルコールとしては、公知のものであれば特に制限されないが、炭素数4〜5の脂肪族アルコールが好ましい。本発明の製造方法において、好ましい脂肪族アルコールとしては、炭素数4のものとして、2−メチル−1−プロパノール、1−ブタノール等が、炭素数5のものとして、3−メチル−1−ブタノール、1−ペンタノール、2−ペンタノール等が挙げられる。これらは1種又は2種以上の組み合せで用いることができる。炭素数4〜5の脂肪族アルコールの含有量は好ましくは0.0002〜0.0007質量%であり、より好ましくは0.0003〜0.0006質量%である。本明細書において、脂肪族アルコールの含有量は、ヘッドスペースガスクロマトグラフ法を用いて測定することができる。 In the production method of the present invention, an aliphatic alcohol may be added from the viewpoint of imparting a feeling of alcohol. The aliphatic alcohol is not particularly limited as long as it is known, but an aliphatic alcohol having 4 to 5 carbon atoms is preferable. In the production method of the present invention, preferred aliphatic alcohols include 2-methyl-1-propanol and 1-butanol as those having 4 carbon atoms, and 3-methyl-1-butanol as those having 5 carbon atoms. Examples thereof include 1-pentanol and 2-pentanol. These can be used in one type or a combination of two or more types. The content of the aliphatic alcohol having 4 to 5 carbon atoms is preferably 0.0002 to 0.0007% by mass, and more preferably 0.0003 to 0.0006% by mass. In the present specification, the content of the aliphatic alcohol can be measured by using a headspace gas chromatograph method.
(酸味料)
本発明の製造方法において使用される酸味料としては、クエン酸、乳酸、リン酸、及びリンゴ酸からなる群より選ばれる1種以上の酸を用いることが好ましい。また、本発明の製造方法においては、前記酸以外の酸として、コハク酸、酒石酸、フマル酸および氷酢酸等も用いることができる。これらは食品に添加することが認められているものであれば制限なく用いることができる。本発明の製造方法においては、まろやかな酸味を適切に付与する観点から乳酸と、やや刺激感のある酸味を適切に付与する観点からリン酸との組み合わせを用いることが好ましい。
(Acidulant)
As the acidulant used in the production method of the present invention, it is preferable to use one or more acids selected from the group consisting of citric acid, lactic acid, phosphoric acid, and malic acid. Further, in the production method of the present invention, succinic acid, tartaric acid, fumaric acid, glacial acetic acid and the like can also be used as the acid other than the acid. These can be used without limitation as long as they are approved to be added to foods. In the production method of the present invention, it is preferable to use a combination of lactic acid from the viewpoint of appropriately imparting a mild acidity and phosphoric acid from the viewpoint of appropriately imparting a slightly irritating acidity.
酸味料の含有量は、本発明の製造方法で得られるビールテイスト飲料中、クエン酸換算で、ビールテイスト感の付与の観点から、200ppm以上が好ましく、550ppm以上がより好ましく、700ppm以上がさらに好ましく、また、酸味の観点から、15000ppm以下が好ましく、5500ppm以下がより好ましく、2000ppm以下がさらに好ましい。従って、本発明において、酸味料の含有量は、クエン酸換算で、200ppm〜15000ppm、好ましくは550ppm〜5500ppm、より好ましくは700ppm〜1500ppmなどの好適範囲が挙げられる。なお、本明細書において、クエン酸換算量とは、クエン酸の酸味度を基準として各酸味料の酸味度から換算される量のことであり、例えば、乳酸100ppmに相当するクエン酸換算量は120ppm、リン酸100ppmに相当するクエン酸換算量は200ppm、リンゴ酸100ppmに相当するクエン酸換算量は125ppmとして換算する。 The content of the acidulant in the beer-taste beverage obtained by the production method of the present invention is preferably 200 ppm or more, more preferably 550 ppm or more, still more preferably 700 ppm or more, in terms of citric acid, from the viewpoint of imparting a beer-taste feeling. Further, from the viewpoint of acidity, 15000 ppm or less is preferable, 5500 ppm or less is more preferable, and 2000 ppm or less is further preferable. Therefore, in the present invention, the content of the acidulant may be in a preferable range of 200 ppm to 15000 ppm, preferably 550 ppm to 5500 ppm, more preferably 700 ppm to 1500 ppm in terms of citric acid. In the present specification, the citric acid conversion amount is an amount converted from the acidity of each acidulant based on the acidity of citric acid. For example, the citric acid conversion amount corresponding to 100 ppm of lactic acid is used. The citric acid conversion amount corresponding to 120 ppm and 100 ppm of phosphoric acid is converted as 200 ppm, and the citric acid conversion amount corresponding to 100 ppm of malic acid is converted as 125 ppm.
ビールテイスト飲料中の酸味料の含有量については、高速液体クロマトグラフィー(HPLC)等により分析して算出されたものを指す。 The content of the acidulant in the beer-taste beverage refers to the one calculated by analysis by high performance liquid chromatography (HPLC) or the like.
(ホップ)
本発明の製造方法においては、原料の一部にホップを用いることができる。香味がビールに類似する傾向にあることから、原料の一部にホップを用いることが望ましい。ホップを使用する際には、ビール等の製造に使用される通常のペレットホップ、粉末ホップ、ホップエキスを、所望の香味に応じて適宜選択して使用することができる。また、イソ化ホップ、還元ホップなどのホップ加工品を用いてもよい。本発明の製造方法に使用されるホップには、これらのものが包含される。また、ホップの添加量は特に限定されないが、典型的には、飲料全量に対して0.0001〜1質量%程度である。
(hop)
In the production method of the present invention, hops can be used as a part of the raw material. Hops are desirable as part of the raw material, as the flavor tends to resemble beer. When hops are used, ordinary pellet hops, powdered hops, and hop extracts used in the production of beer and the like can be appropriately selected and used according to a desired flavor. Further, processed hop products such as isometric hops and reduced hops may be used. These are included in the hops used in the production method of the present invention. The amount of hops added is not particularly limited, but is typically about 0.0001 to 1% by mass with respect to the total amount of the beverage.
(その他の原料)
本発明の製造方法においては、任意に、その他の原料を用いてもよい。例えば、甘味料(高甘味度甘味料を含む)、苦味料、香料、酵母エキス、カラメル色素などの着色料、大豆サポニンやキラヤサポニン等の植物抽出サポニン系物質、コーンや大豆などの植物タンパク質およびペプチド含有物、乳清などの動物タンパク質、食物繊維やアミノ酸などの調味料、アスコルビン酸等の酸化防止剤を、本発明の効果を妨げない範囲で必要に応じて用いることができる。
(Other raw materials)
In the production method of the present invention, other raw materials may be optionally used. For example, sweeteners (including high-sweetness sweeteners), bitterness agents, flavors, yeast extracts, colorants such as caramel color, plant-extracted saponin-based substances such as soybean saponin and Kiraya saponin, plant proteins such as corn and soybean, and Peptide-containing substances, animal proteins such as milk syrup, seasonings such as dietary fiber and amino acids, and antioxidants such as ascorbic acid can be used as needed as long as the effects of the present invention are not impaired.
本発明の製造方法で得られるビールテイスト飲料のpHは、飲料の風味を良好にする観点から、好ましくは3.0〜5.0であり、より好ましくは3.0〜4.5であり、更に好ましくは3.0〜4.0である。 The pH of the beer-taste beverage obtained by the production method of the present invention is preferably 3.0 to 5.0, more preferably 3.0 to 4.5, from the viewpoint of improving the flavor of the beverage. More preferably, it is 3.0 to 4.0.
(容器詰飲料)
本発明の製造方法で得られるビールテイスト飲料は、容器詰めとすることができる。容器の形態は何ら制限されず、ビン、缶、樽、またはペットボトル等の密封容器に充填して、容器入り飲料とすることができる。
(Beverage in a container)
The beer-taste beverage obtained by the production method of the present invention can be packaged. The form of the container is not limited in any way, and it can be filled in a sealed container such as a bottle, a can, a barrel, or a PET bottle to obtain a beverage in a container.
以下、実施例を示して本発明を具体的に説明するが、本発明は下記実施例に制限されるものではない。なお、プリン体除去工程において特に加圧した旨の記載がないものについては、常圧(101.3kPa)にて行った。 Hereinafter, the present invention will be specifically described with reference to Examples, but the present invention is not limited to the following Examples. In the purine body removing step, those without a description of pressurization were carried out at normal pressure (101.3 kPa).
調製例:麦汁の調製
麦芽とホップ、水、糖類、食物繊維を混合して麦汁を得た。各試験区において、以下の通り発酵と吸着剤の接触を行った後、吸着剤濃度の測定を行った。その後濾過によってビールテイスト飲料を得た後、ビールテイスト飲料を過塩素酸により加水分解したサンプル中のキサンチン、アデニン、グアニンの分析を行った。さらに香気成分である酪酸エチル及びカプロン酸エチルの含有量を、BCOJビール分析法(財団法人日本醸造協会発行、ビール酒造組合国際技術委員会(分析委員会)編集1996年4月1日発行)の「8.22 低沸点香気成分」の方法にしたがい、FID検出器付きガスクロマトグラフを用いて測定した。
Preparation example: Preparation of wort Wort was obtained by mixing malt with hops, water, sugars and dietary fiber. In each test group, the concentration of the adsorbent was measured after the fermentation and the contact of the adsorbent were carried out as follows. Then, after obtaining a beer-taste beverage by filtration, xanthine, adenine, and guanine in a sample obtained by hydrolyzing the beer-taste beverage with perchloric acid were analyzed. Furthermore, the content of ethyl butyrate and ethyl caproate, which are aroma components, is determined by the BCOJ Beer Analysis Method (published by the Brewing Society of Japan, edited by the International Technical Committee of the Brewing Society of Japan (Analysis Committee), published on April 1, 1996). According to the method of "8.22 low boiling point aroma component", the measurement was performed using a gas chromatograph with a FID detector.
比較例1
調製例で得られた麦汁に酵母を加えて7日間発酵させた。その後遠心によって酵母を取り除いた後、3時間静置した。その後濾過を行い、ビールテイスト飲料を得た。
Comparative Example 1
Yeast was added to the wort obtained in the preparation example and fermented for 7 days. Then, after removing the yeast by centrifugation, it was allowed to stand for 3 hours. After that, filtration was performed to obtain a beer-taste beverage.
比較例2
調製例で得られた麦汁に酵母とヌクレオシダーゼを加えて7日間発酵と酵素剤処理を行った。その後遠心によって酵母を取り除いた後、3時間静置した。その後濾過を行い、ビールテイスト飲料を得た。
Comparative Example 2
Yeast and nucleosidase were added to the wort obtained in the preparation example, and fermentation and enzyme treatment were carried out for 7 days. Then, after removing the yeast by centrifugation, it was allowed to stand for 3 hours. After that, filtration was performed to obtain a beer-taste beverage.
比較例3
調製例で得られた麦汁に酵母とヌクレオシダーゼを加えて7日間発酵と酵素剤処理を行った。その後遠心によって酵母を取り除いた後、活性炭を3000ppmとなるように加え、活性炭が均一となるように軽く撹拌を行った。その後、3時間静置して活性炭と接触させた。3時間の接触後、一部サンプルを採取し分散率測定用のサンプルとした。その後濾過を行い、活性炭を除いてビールテイスト飲料を得た。
Comparative Example 3
Yeast and nucleosidase were added to the wort obtained in the preparation example, and fermentation and enzyme treatment were carried out for 7 days. Then, after removing the yeast by centrifugation, activated carbon was added so as to be 3000 ppm, and the mixture was lightly stirred so that the activated carbon became uniform. Then, it was allowed to stand for 3 hours and brought into contact with activated carbon. After contact for 3 hours, a part of the sample was taken and used as a sample for measuring the dispersion rate. After that, filtration was performed to remove activated carbon to obtain a beer-taste beverage.
実施例1
調製例で得られた麦汁に酵母とヌクレオシダーゼを加えて7日間発酵と酵素剤処理を行った。その後遠心によって酵母を取り除いた後、活性白土を3000ppmとなるように加え、活性白土が均一となるように軽く撹拌を行った。その後、3時間静置して活性白土と接触させた。3時間の接触後、一部サンプルを採取し分散率測定用のサンプルとした。その後濾過を行い、活性白土を除いてビールテイスト飲料を得た。
Example 1
Yeast and nucleosidase were added to the wort obtained in the preparation example, and fermentation and enzyme treatment were carried out for 7 days. Then, after removing the yeast by centrifugation, active clay was added so as to be 3000 ppm, and the mixture was lightly stirred so that the active clay became uniform. Then, it was allowed to stand for 3 hours and brought into contact with active clay. After contact for 3 hours, a part of the sample was taken and used as a sample for measuring the dispersion rate. After that, filtration was performed to remove active clay to obtain a beer-taste beverage.
実施例2
調製例で得られた麦汁に酵母とヌクレオシダーゼを加えて7日間発酵と酵素剤処理を行った。その後遠心によって酵母を取り除いた後、活性白土を3000ppmとなるように加え、活性白土が均一となるように軽く撹拌を行った。その後、炭酸ガスを容器下部から吹き込み3時間分散させながら接触させた。3時間の接触後、一部サンプルを採取し分散率測定用のサンプルとした。その後濾過を行い、活性白土を除いてビールテイスト飲料を得た。
Example 2
Yeast and nucleosidase were added to the wort obtained in the preparation example, and fermentation and enzyme treatment were carried out for 7 days. Then, after removing the yeast by centrifugation, active clay was added so as to be 3000 ppm, and the mixture was lightly stirred so that the active clay became uniform. Then, carbon dioxide gas was blown from the lower part of the container and brought into contact with each other while being dispersed for 3 hours. After contact for 3 hours, a part of the sample was taken and used as a sample for measuring the dispersion rate. After that, filtration was performed to remove active clay to obtain a beer-taste beverage.
実施例3
調製例で得られた麦汁に酵母とヌクレオシダーゼを加えて7日間発酵と酵素剤処理を行った。その後遠心によって酵母を取り除いた後、活性白土を3000ppmとなるように加え、活性白土が均一となるように軽く撹拌を行った。その後、大気圧に加えてさらに1.5kPa加圧し、加圧条件のもと、炭酸ガスを容器下部から吹き込み3時間分散させながら接触させた。3時間の接触後、一部サンプルを採取し分散率測定用のサンプルとした。その後濾過を行い、活性白土を除いてビールテイスト飲料を得た。
Example 3
Yeast and nucleosidase were added to the wort obtained in the preparation example, and fermentation and enzyme treatment were carried out for 7 days. Then, after removing the yeast by centrifugation, active clay was added so as to be 3000 ppm, and the mixture was lightly stirred so that the active clay became uniform. Then, the pressure was further increased by 1.5 kPa in addition to the atmospheric pressure, and under the pressure condition, carbon dioxide gas was blown from the lower part of the container and brought into contact with each other while being dispersed for 3 hours. After contact for 3 hours, a part of the sample was taken and used as a sample for measuring the dispersion rate. After that, filtration was performed to remove active clay to obtain a beer-taste beverage.
<分散率の測定>
実施例1〜3、比較例3で得られた分散率測定用のサンプルは660nmでの透過度測定に供した。
<白土の分散率の測定>
あらかじめ既知濃度の白土を懸濁したサンプルを用いて、透過度と白土濃度との検量線を作成した。実施例1では3時間の静置後に、実施例2、3では分散処理の終了直前に液面の高さの半分の位置からサンプリングを行いサンプルとした。透過度測定の際には測定の直前にサンプルを均一に撹拌した。得られた透過度の結果を検量線に当てはめてサンプル中の白土濃度とした。得られた白土濃度と添加した白土濃度から、以下の式に基づいて分散率を算出した。
白土の分散率(%)=100×サンプル中の白土濃度/添加した白土濃度
<活性炭の分散率の測定>
粒状の活性炭をハンマーで粉末状に砕き、それを用いてあらかじめ既知濃度の活性炭を懸濁したサンプルを用いて、透過度と活性炭濃度との検量線を作成した。比較例3では3時間の静置の終了直前に液面の高さの半分の位置からサンプリングを行った。サンプリングした液は遠心した後に上清を取り除き、残った活性炭をハンマーで粉末状に砕いた。それらをもとの上清に戻し入れたものをサンプルとした。透過度測定の際には測定の直前にサンプルを均一に撹拌した。得られた透過度を検量線に当てはめてサンプル中の活性炭濃度とした。得られた活性炭濃度と添加した活性炭濃度から、以下の式に基づいて分散率を算出した。
活性炭の分散率(%)=100×サンプル中の活性炭濃度/添加した活性炭濃度
<Measurement of dispersion rate>
The samples for measuring the dispersibility obtained in Examples 1 to 3 and Comparative Example 3 were subjected to the transmittance measurement at 660 nm.
<Measurement of dispersion rate of white clay>
A calibration curve of permeability and white clay concentration was prepared using a sample in which white clay having a known concentration was suspended in advance. In Example 1, after standing for 3 hours, in Examples 2 and 3, sampling was performed from a position half the height of the liquid surface immediately before the end of the dispersion treatment to prepare a sample. When measuring the permeability, the sample was uniformly stirred immediately before the measurement. The result of the obtained transmittance was applied to the calibration curve to obtain the white clay concentration in the sample. From the obtained white clay concentration and the added white clay concentration, the dispersion rate was calculated based on the following formula.
Dispersion rate of white clay (%) = 100 x concentration of white clay in sample / concentration of white clay added <measurement of dispersion rate of activated carbon>
Granular activated carbon was crushed into powder with a hammer, and a calibration curve of permeability and activated carbon concentration was prepared using a sample in which activated carbon of a known concentration was suspended in advance. In Comparative Example 3, sampling was performed from a position half the height of the liquid level immediately before the end of standing for 3 hours. The sampled liquid was centrifuged, the supernatant was removed, and the remaining activated carbon was crushed into powder with a hammer. A sample was prepared by returning them to the original supernatant. When measuring the permeability, the sample was uniformly stirred immediately before the measurement. The obtained permeability was applied to the calibration curve to obtain the activated carbon concentration in the sample. From the obtained activated carbon concentration and the added activated carbon concentration, the dispersion ratio was calculated based on the following formula.
Dispersion rate of activated carbon (%) = 100 x concentration of activated carbon in sample / concentration of added activated carbon
表1より、酵素処理後のプリン体除去工程に白土を用いた実施例1〜3は、活性炭を用いた比較例3に比べて、いずれもキサンチン、アデニン、グアニンの量が少なく、プリン体除去効率に優れるものであったことが分かる。白土を分散させた実施例2、3では特にプリン体除去効率に優れており、さらに加圧下でプリン体除去工程を行った実施例3においては香気成分の減少も抑制されており、香気に優れるものであった。また、より高い加圧条件下(常圧+100kPa)で活性白土を分散接触させた場合も同様の効果を確認できた。 From Table 1, in Examples 1 to 3 using white clay in the purine body removing step after the enzyme treatment, the amounts of xanthine, adenine, and guanine were smaller than those in Comparative Example 3 using activated carbon, and purine bodies were removed. It can be seen that it was excellent in efficiency. In Examples 2 and 3 in which the white clay was dispersed, the purine body removing efficiency was particularly excellent, and in Example 3 in which the purine body removing step was performed under pressure, the decrease in the aroma component was also suppressed, and the aroma was excellent. It was a thing. In addition, the same effect could be confirmed when the activated clay was dispersed and contacted under higher pressure conditions (normal pressure + 100 kPa).
本発明の製造方法によれば、吸着剤の使用量が少なく、コストや生産性に優れたビールテイスト飲料の製造方法を提供することができる。 According to the production method of the present invention, it is possible to provide a production method of a beer-taste beverage which uses a small amount of an adsorbent and is excellent in cost and productivity.
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JP2021141839A (en) * | 2020-03-11 | 2021-09-24 | サントリーホールディングス株式会社 | Beer taste beverage |
JP2021141838A (en) * | 2020-03-11 | 2021-09-24 | サントリーホールディングス株式会社 | Beer taste beverage |
DE112022003373T5 (en) | 2021-08-31 | 2024-04-18 | Murata Manufacturing Co., Ltd. | Electronic component |
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WO1996025483A1 (en) * | 1995-02-17 | 1996-08-22 | Suntory Limited | Process for producing beer |
JP2003169658A (en) * | 2001-09-27 | 2003-06-17 | Kirin Brewery Co Ltd | Method for producing fermented malt beverage |
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JP2012125205A (en) * | 2010-12-16 | 2012-07-05 | Kirin Brewery Co Ltd | Purine base compound-reduced, aromatic beer-flavored fermented alcoholic beverage, and method for producing the same |
JP2018064503A (en) * | 2016-10-19 | 2018-04-26 | アサヒビール株式会社 | Manufacturing method of beer-like sparkling beverage |
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WO1996025483A1 (en) * | 1995-02-17 | 1996-08-22 | Suntory Limited | Process for producing beer |
JP2003169658A (en) * | 2001-09-27 | 2003-06-17 | Kirin Brewery Co Ltd | Method for producing fermented malt beverage |
JP2004321004A (en) * | 2003-04-21 | 2004-11-18 | Asahi Breweries Ltd | Method for producing fermented malt beverage |
JP2012125205A (en) * | 2010-12-16 | 2012-07-05 | Kirin Brewery Co Ltd | Purine base compound-reduced, aromatic beer-flavored fermented alcoholic beverage, and method for producing the same |
JP2018064503A (en) * | 2016-10-19 | 2018-04-26 | アサヒビール株式会社 | Manufacturing method of beer-like sparkling beverage |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
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JP2021141839A (en) * | 2020-03-11 | 2021-09-24 | サントリーホールディングス株式会社 | Beer taste beverage |
JP2021141838A (en) * | 2020-03-11 | 2021-09-24 | サントリーホールディングス株式会社 | Beer taste beverage |
DE112022003373T5 (en) | 2021-08-31 | 2024-04-18 | Murata Manufacturing Co., Ltd. | Electronic component |
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