JP2021023207A - Production method of fermented milk, and method of suppressing water separation from fermented milk - Google Patents
Production method of fermented milk, and method of suppressing water separation from fermented milk Download PDFInfo
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Abstract
Description
本発明は発酵乳の製造方法及び発酵乳の離水の抑制方法に関する。 The present invention relates to a method for producing fermented milk and a method for suppressing water separation of fermented milk.
通常、発酵乳を製造する場合の原料液の加熱処理条件としては、(i)温度85℃で30分間、(ii)温度90〜95℃で10分〜5分間、(iii)温度120℃で5〜3秒間、が一般的に知られている(非特許文献1)。 Usually, the conditions for heat treatment of the raw material liquid for producing fermented milk are (i) temperature 85 ° C. for 30 minutes, (ii) temperature 90 to 95 ° C. for 10 minutes to 5 minutes, and (iii) temperature 120 ° C. 5 to 3 seconds is generally known (Non-Patent Document 1).
健康志向が高まるなかで、各種の効能成分を含有する発酵乳が開発されている。
本発明者等は、水溶性食物繊維である難消化性デキストリンを添加した原料液を発酵させる方法で発酵乳を製造すると、離水が生じやすいことを知見した。
本発明は、難消化性デキストリンを含みながら、離水が生じ難い発酵乳の製造方法、及び発酵乳の離水の抑制方法を提供する。
Amid growing health consciousness, fermented milk containing various efficacy ingredients has been developed.
The present inventors have found that when fermented milk is produced by a method of fermenting a raw material liquid to which indigestible dextrin, which is a water-soluble dietary fiber, is added, water separation is likely to occur.
The present invention provides a method for producing fermented milk, which contains indigestible dextrin but is unlikely to cause water separation, and a method for suppressing water separation from fermented milk.
本発明者等は、鋭意研究した結果、乳原料と難消化性デキストリンを含む原料液を熱処理すると、熱処理後に褐変(着色)が生じやすいこと、褐変した場合に発酵後の離水が生じやすいこと、熱処理条件を調整して褐変の程度を制御することによって発酵後の離水を抑制できることを見出して本発明に至った。 As a result of diligent research, the present inventors have found that when a raw material solution containing a dairy raw material and indigestible dextrin is heat-treated, browning (coloring) is likely to occur after the heat treatment, and when browning occurs, water separation after fermentation is likely to occur. The present invention has been made by finding that water separation after fermentation can be suppressed by adjusting the heat treatment conditions to control the degree of browning.
本発明は以下の態様を有する。
[1] 乳原料と難消化性デキストリンを含む原料液に、加熱温度80℃以上かつ保持時間1分間以上の熱処理を施した後、乳酸菌を添加し、発酵させて発酵乳を得る方法であって、前記熱処理を施す前の前記原料液の、CIE L*a*b*表色系におけるb*値をb1、前記乳酸菌添加時の前記原料液の前記b*値をb2とすると、b2/b1の比が1.95以下となるように前記熱処理を行う、発酵乳の製造方法。
[2] 前記原料液の総質量に対して、前記難消化性デキストリンの含有量が2.5〜7質量%である、[1]の製造方法。
[3] 前記原料液の総質量に対して、無脂乳固形分が8質量%以上である、[1]又は[2]の製造方法。
[4] 前記熱処理の加熱温度が80〜96℃であり、かつ保持時間が1〜10分間である、[1]〜[3]のいずれかの製造方法。
[5] 乳原料と難消化性デキストリンを含む原料液に、加熱温度80℃以上かつ保持時間1分間以上の熱処理を施した後、乳酸菌を添加して発酵させた発酵乳の離水を抑制する方法であって、前記熱処理を施す前の前記原料液の、CIE L*a*b*表色系におけるb*値をb1、前記乳酸菌添加時の前記原料液の前記b*値をb2とすると、b2/b1の比が1.95以下となるように前記熱処理を行う、発酵乳の離水の抑制方法。
The present invention has the following aspects.
[1] A method in which a raw material solution containing a milk raw material and indigestible dextrin is heat-treated at a heating temperature of 80 ° C. or higher and a holding time of 1 minute or longer, and then lactic acid bacteria are added and fermented to obtain fermented milk. Assuming that the b * value of the raw material liquid before the heat treatment is b1 in the CIE L * a * b * color system and the b * value of the raw material liquid at the time of adding the lactic acid bacteria is b2, b2 / b1 A method for producing fermented milk, wherein the heat treatment is performed so that the ratio of lactic acid is 1.95 or less.
[2] The production method according to [1], wherein the content of the indigestible dextrin is 2.5 to 7% by mass with respect to the total mass of the raw material liquid.
[3] The production method of [1] or [2], wherein the non-fat milk solid content is 8% by mass or more with respect to the total mass of the raw material liquid.
[4] The production method according to any one of [1] to [3], wherein the heating temperature of the heat treatment is 80 to 96 ° C. and the holding time is 1 to 10 minutes.
[5] A method for suppressing water separation of fermented milk fermented by adding lactic acid bacteria after heat treatment of a raw material solution containing a milk raw material and indigestible dextrin at a heating temperature of 80 ° C. or higher and a holding time of 1 minute or longer. It is assumed that the b * value of the raw material liquid before the heat treatment is b1 in the CIE L * a * b * color system and the b * value of the raw material liquid at the time of adding the lactic acid bacteria is b2. A method for suppressing water separation of fermented milk, wherein the heat treatment is performed so that the ratio of b2 / b1 is 1.95 or less.
本発明によれば、難消化性デキストリンを含みながら、離水が生じ難い発酵乳を製造できる。 According to the present invention, fermented milk containing indigestible dextrin but less likely to cause water separation can be produced.
本明細書において「〜」で表される数値範囲は、〜の前後の数値を下限値及び上限値とする数値範囲を意味する。
本明細書において、CIE L*a*b*表色系におけるb*値は、国際照明委員会(CIE)で規定される色差式(CIE L*a*b*表色系)による色度(a*値、b*値、L*値)の一つである。CIE L*a*b*表色系において、L*軸は明るさを表す明度軸であり、0に近いと黒、100に近いと白を表す。a*軸は緑〜赤を表し、マイナスは緑、プラスは赤を表す。b*軸は青〜黄を表し、マイナスは青、プラスは黄を表す。
In the present specification, the numerical range represented by "~" means a numerical range in which the numerical values before and after ~ are the lower limit value and the upper limit value.
In the present specification, the b * value in the CIE L * a * b * color system is the chromaticity (CIE L * a * b * color system) defined by the International Commission on Illumination (CIE). It is one of a * value, b * value, L * value). In the CIE L * a * b * color system, the L * axis is the brightness axis indicating brightness, and when it is close to 0, it represents black, and when it is close to 100, it represents white. The a * axis represents green to red, minus represents green, and plus represents red. The b * axis represents blue to yellow, minus represents blue, and plus represents yellow.
発酵乳には、製法の違いにより撹拌型発酵乳と静置型発酵乳がある。撹拌型発酵乳(前発酵型発酵乳とも呼ばれる。)は、発酵乳原料をタンク内等で発酵させ、形成されたカードを撹拌、粉砕して流動性を持たせ、製品容器(飲食用の容器)に充填したものである。
静置型発酵乳(後発酵型発酵乳とも呼ばれる。)は、発酵乳原料を製品容器(飲食用の容器)に充填した後に発酵させ、容器内で形成されたカードを撹拌することなく市販に供するもので、流動性を有しない固形状態の発酵乳(カード)が最終製品となる。
本実施形態の発酵乳の製造方法は、静置型発酵乳であっても、撹拌型発酵乳であっても適用できる。
Fermented milk includes agitated fermented milk and static fermented milk depending on the manufacturing method. Stirring fermented milk (also called pre-fermented fermented milk) is made by fermenting fermented milk raw materials in a tank or the like, stirring and crushing the formed curd to make it fluid, and making it a product container (container for eating and drinking). ) Is filled.
In static fermented milk (also called post-fermented fermented milk), the fermented milk raw material is filled in a product container (container for eating and drinking) and then fermented, and the curd formed in the container is put on the market without stirring. The final product is fermented milk (curd) in a solid state that does not have fluidity.
The method for producing fermented milk of the present embodiment can be applied to either static fermented milk or agitated fermented milk.
<難消化性デキストリン>
本実施形態において用いられる難消化性デキストリンは、澱粉から得られる水溶性の食物繊維の一種である。「難消化性」とは、ヒトの消化酵素で消化されにくいことをいう。
例えば、とうもろこし、小麦、大麦、米、豆類、イモ類(馬鈴薯、甘藷)、タピオカなどの植物由来の澱粉を加酸(鉱酸を添加)及び/又は加熱して得た焙焼デキストリンを、必要に応じてαアミラーゼ及び/又はグルコアミラーゼで酵素処理した後、必要に応じて脱塩、脱色した水溶性食物繊維であり、難消化性の特徴を有する。
<Indigestible dextrin>
The indigestible dextrin used in this embodiment is a kind of water-soluble dietary fiber obtained from starch. "Indigestible" means that it is difficult to be digested by human digestive enzymes.
For example, roasted dextrin obtained by acidifying (adding mineral acid) and / or heating plant-derived starch such as corn, wheat, barley, rice, beans, potatoes (potato, sweet potato), tapioca is required. It is a water-soluble dietary fiber that has been enzymatically treated with α-amylase and / or glucoamylase, and then desalted and decolorized as necessary, and has a characteristic of being indigestible.
難消化性デキストリンは、低カロリーで低脂肪の食品素材であり、整腸作用、血糖上昇抑制作用、血清コレステロール低下作用、腸内環境改善、中性脂肪低下作用等の生理活性効果を有しているとされている(例えば、参考文献1(特開2001−252064号公報)、参考文献2(特開平04−1591765号公報等)。
難消化性デキストリンの製造方法は、例えば、参考文献1及び参考文献2を参照できる。難消化性デキストリンの原料である植物由来の澱粉は特に限定されないが、特にトウモロコシ澱粉が好適である。加酸するときの鉱酸として、例えば、塩酸、硝酸、硫酸等が挙げられ、このうち塩酸が好適である。
Indigestible dextrin is a low-calorie, low-fat food material that has physiologically active effects such as intestinal regulation, blood sugar elevation suppression, serum cholesterol lowering, intestinal environment improvement, and triglyceride lowering. (For example, Reference 1 (Japanese Patent Laid-Open No. 2001-52064), Reference 2 (Japanese Patent Laid-Open No. 04-1591765, etc.).
For the method for producing indigestible dextrin, for example, Reference 1 and Reference 2 can be referred to. The plant-derived starch that is the raw material of the indigestible dextrin is not particularly limited, but corn starch is particularly preferable. Examples of the mineral acid at the time of acidification include hydrochloric acid, nitric acid, sulfuric acid and the like, of which hydrochloric acid is preferable.
難消化性デキストリンには、平成11年4月26日付衛新第13号(「栄養表示基準における栄養成分等の分析方法等について」)に記載の食物繊維の分析方法である高速液体クロマトグラフ法(酵素−HPLC法)で測定される難消化性成分を含むデキストリン、好ましくは85〜95重量%の難消化性成分を含むデキストリンなどが含まれる。
本実施形態で用いる難消化性デキストリンには、便宜上、水素添加により製造される、難消化性デキストリンの還元物も含まれるものとする。
難消化性デキストリンやその還元物(還元難消化性デキストリン)は、粉末、細粒、顆粒などの形態で市販されており、いずれの形態のものも使用できる。
For indigestible dextrin, high performance liquid chromatograph method, which is an analysis method of dietary fiber described in Eshin No. 13 (“Analysis method of nutritional components, etc. in nutrition labeling standards”) dated April 26, 1999. A dextrin containing an indigestible component measured by (enzyme-HPLC method), preferably a dextrin containing 85 to 95% by weight of the indigestible component, and the like are included.
For convenience, the indigestible dextrin used in the present embodiment also includes a reduced product of the indigestible dextrin produced by hydrogenation.
Indigestible dextrin and its reduced product (reduced indigestible dextrin) are commercially available in the form of powder, fine granules, granules and the like, and any form can be used.
<発酵乳の製造方法>
[原料液の調製]
本実施形態の発酵乳の製造方法は、原料液に乳酸菌を添加し、発酵させて発酵乳を製造する。
まず、乳原料と難消化性デキストリンを含む原料液を調製する。具体的には、水に、乳原料、難消化性デキストリン、および必要に応じたその他の成分を混合して原料液を得る。
本明細書において、原料液とは、少なくとも乳原料と乳原料以外の原料の1種以上とを混合してから、乳酸菌を添加するまでの液を意味する。
<Manufacturing method of fermented milk>
[Preparation of raw material solution]
In the method for producing fermented milk of the present embodiment, lactic acid bacteria are added to the raw material liquid and fermented to produce fermented milk.
First, a raw material solution containing a dairy raw material and indigestible dextrin is prepared. Specifically, a raw material liquid is obtained by mixing a milk raw material, indigestible dextrin, and other components as required with water.
In the present specification, the raw material liquid means a liquid from at least one or more of a dairy raw material and a raw material other than the dairy raw material mixed until lactic acid bacteria are added.
乳原料は乳由来の原料であり、発酵乳の製造において用いられる公知の乳原料を用いることができる。例えば、牛乳、水牛乳、羊乳、山羊乳、馬乳、脱脂乳、脱脂濃縮乳、脱脂粉乳、濃縮乳、全脂粉乳、クリーム、バター、バターミルク、練乳、乳タンパク質が挙げられる。これらは1種類のみ用いてもよいし、2種類以上を混合して用いてもよい。
乳原料の含有量は、原料液の総質量に対して、無脂乳固形分が8質量%以上であることが好ましく、12質量%以下が好ましく、8〜10質量%がより好ましい。上記範囲の下限値以上であると適度な乳味を呈し、良好な風味が得られやすい。上限値以下であると加熱殺菌による乳タンパク質の凝集が抑えられやすい。
The dairy raw material is a milk-derived raw material, and a known dairy raw material used in the production of fermented milk can be used. For example, milk, buffalo milk, sheep milk, goat milk, horse milk, defatted milk, defatted concentrated milk, defatted powdered milk, concentrated milk, whole fat powdered milk, cream, butter, butter milk, condensed milk, milk protein can be mentioned. Only one type of these may be used, or two or more types may be mixed and used.
The content of the dairy raw material is preferably 8% by mass or more, preferably 12% by mass or less, and more preferably 8 to 10% by mass, based on the total mass of the raw material liquid. When it is at least the lower limit of the above range, an appropriate milky taste is exhibited, and a good flavor can be easily obtained. When it is less than the upper limit, aggregation of milk protein due to heat sterilization is likely to be suppressed.
難消化性デキストリンの含有量は、原料液の総質量に対して2.5〜7質量%が好ましく、3〜6質量%がより好ましい。上記範囲の下限値以上であると生理的効果が期待でき、上限値以下であると好ましい風味が得られやすい。 The content of the indigestible dextrin is preferably 2.5 to 7% by mass, more preferably 3 to 6% by mass, based on the total mass of the raw material liquid. If it is at least the lower limit of the above range, a physiological effect can be expected, and if it is at least the upper limit, a preferable flavor can be easily obtained.
原料液は上記乳原料及び難消化性デキストリン以外に水を含む。さらに本発明の効果を損なわない範囲でその他の成分を含むことができる。
その他の成分として、例えば、希少糖、フルクトース、プシコース、アロース、ラクチュロース、ショ糖等の糖類、低カロリー甘味料、カゼイン加水分解物、植物性脂肪、安定剤(寒天、ゼラチン、ペクチン等)、香料、pH調整剤等が挙げられる。
希少糖とは、一般に、自然界での存在量が少ない単糖や糖アルコールのことを指す。
低カロリー甘味料としては、アスパルテーム、アセスルファムK、スクラロース(別名:4,1’,6’−トリクロロガラクトスクロース)、ステビア甘味料、サッカリン、サッカリン酸ナトリウム、カンゾウ抽出物等が挙げられる。
The raw material liquid contains water in addition to the above dairy raw material and indigestible dextrin. Further, other components can be contained as long as the effects of the present invention are not impaired.
Other ingredients include, for example, rare sugars, fructose, psicose, allose, lactulose, sucrose and other sugars, low-calorie sweeteners, casein hydrolysates, vegetable fats, stabilizers (agar, gelatin, pectin, etc.), fragrances. , PH adjuster and the like.
Rare sugars generally refer to monosaccharides and sugar alcohols that are abundant in nature.
Examples of low-calorie sweeteners include aspartame, acesulfame K, sucralose (also known as 4,1', 6'-trichlorogalactosucrose), stevia sweeteners, saccharin, sodium saccharinate, and citrus extract.
原料液の25℃におけるpHは6.4〜6.9が好ましく、6.5〜6.7がより好ましい。原料液のpHが上記範囲であると、原料液が加熱されたときに凝集が生じにくい。 The pH of the raw material solution at 25 ° C. is preferably 6.4 to 6.9, more preferably 6.5 to 6.7. When the pH of the raw material liquid is in the above range, aggregation is unlikely to occur when the raw material liquid is heated.
[熱処理]
原料液は、乳酸菌を添加する前に、1回以上の熱処理が施される。
本明細書における熱処理は、加熱温度が80℃以上であり、かつ保持時間が1分間以上である処理を意味する。保持時間は、所定の加熱温度に保持される時間を意味する。所定の加熱温度までの昇温時間や降温時間は保持時間に含まれない。
原料液の熱処理条件が、加熱温度80℃以上かつ保持時間1分間以上であると、熱処理後に褐変(着色)が生じやすく、発酵後の離水が生じやすいため、本発明を適用することの効果が大きい。
[Heat treatment]
The raw material liquid is heat-treated at least once before adding lactic acid bacteria.
The heat treatment in the present specification means a treatment in which the heating temperature is 80 ° C. or higher and the holding time is 1 minute or longer. The holding time means the time for holding at a predetermined heating temperature. The holding time does not include the temperature raising time and the temperature lowering time up to the predetermined heating temperature.
When the heat treatment conditions of the raw material liquid are a heating temperature of 80 ° C. or higher and a holding time of 1 minute or longer, browning (coloring) is likely to occur after the heat treatment and water separation after fermentation is likely to occur. Therefore, the effect of applying the present invention is effective. large.
本実施形態において、前記熱処理を2回以上行ってもよい。例えば、80℃以上である第1の加熱温度に1分間以上保持した後、降温し、次いで80℃以上である第2の加熱温度に1分間以上保持した後、降温してもよい。第1の加熱温度と第2の加熱温度とは同じであってもよく、異なってもよい。前記熱処理の少なくとも1回は、加熱殺菌処理であることが好ましい。
前記熱処理の加熱温度が高すぎると、又は保持時間が長すぎると発酵遅延、離水が生じやすい点で好ましくない。例えば加熱温度の上限は96℃以下が好ましく、90℃以下がより好ましい。保持時間の上限は10分間以下が好ましい。
前記熱処理の加熱温度の下限は80℃以上であるが、発酵乳の組織形成の点では85℃以上が好ましい。
In this embodiment, the heat treatment may be performed twice or more. For example, the temperature may be lowered after being held at a first heating temperature of 80 ° C. or higher for 1 minute or longer, and then kept at a second heating temperature of 80 ° C. or higher for 1 minute or longer. The first heating temperature and the second heating temperature may be the same or different. It is preferable that at least one of the heat treatments is a heat sterilization treatment.
If the heating temperature of the heat treatment is too high or the holding time is too long, fermentation delay and water separation are likely to occur, which is not preferable. For example, the upper limit of the heating temperature is preferably 96 ° C. or lower, more preferably 90 ° C. or lower. The upper limit of the holding time is preferably 10 minutes or less.
The lower limit of the heating temperature of the heat treatment is 80 ° C. or higher, but 85 ° C. or higher is preferable from the viewpoint of tissue formation of fermented milk.
本実施形態において、熱処理の加熱温度と保持時間は、熱処理を施す前の原料液のb*値をb1、乳酸菌添加時の原料液のb*値をb2とすると、b2/b1の比が1.95以下となるように設定する。
本明細書において、乳酸菌添加時とは、乳酸菌を添加する直前、添加と同時、及び添加した直後を含む概念である。
原料液の熱処理によって褐変(着色)が生じるとb1よりもb2が大きくなる。加熱温度が同じであれば保持時間が長いほど、b2/b1の比が大きくなる傾向がある。保持時間が同じであれば、加熱温度が高いほど、b2/b1の比が大きくなる傾向がある。
b2/b1の比が1.95以下であると離水抑制効果に優れる。b2/b1の比は1.8以下が好ましく、1.6以下がより好ましい。b2/b1の比の下限は1超であり、特に限定されない。現実的には1.1以上が好ましい。
In the present embodiment, the heating temperature and holding time of the heat treatment are such that the ratio of b2 / b1 is 1 when the b * value of the raw material liquid before the heat treatment is b1 and the b * value of the raw material liquid when the lactic acid bacteria are added is b2. Set so that it is .95 or less.
In the present specification, the term "when lactic acid bacteria are added" is a concept including immediately before, at the same time as, and immediately after the addition of lactic acid bacteria.
When browning (coloring) occurs due to the heat treatment of the raw material liquid, b2 becomes larger than b1. If the heating temperature is the same, the longer the holding time, the larger the ratio of b2 / b1 tends to be. If the holding time is the same, the higher the heating temperature, the larger the ratio of b2 / b1 tends to be.
When the ratio of b2 / b1 is 1.95 or less, the effect of suppressing water separation is excellent. The ratio of b2 / b1 is preferably 1.8 or less, more preferably 1.6 or less. The lower limit of the ratio of b2 / b1 is more than 1, and is not particularly limited. In reality, 1.1 or more is preferable.
b2の値は、1〜8が好ましく、3〜7がより好ましい。上記範囲の下限値以上であると好ましい乳の風味が得られやすく、上限値以下であると発酵遅延、離水を抑制しやすい。
b1の値は、原料液の組成によって変化する。原料のロットによっても変化する。
The value of b2 is preferably 1 to 8, and more preferably 3 to 7. If it is at least the lower limit of the above range, a preferable milk flavor can be easily obtained, and if it is at least the upper limit, fermentation delay and water separation can be easily suppressed.
The value of b1 changes depending on the composition of the raw material liquid. It also changes depending on the lot of raw materials.
本実施形態において、乳酸菌を添加する前の原料液に、前記熱処理を1回以上行うほかに、必要に応じて、前記熱処理以外の他の熱処理を1回以上行ってもよい。他の熱処理は前記熱処理の前でもよく後でもよい。
例えば、加熱温度が80℃以上であっても、保持時間が1分間未満である加熱工程は、他の熱処理である。また加熱温度が80℃未満であれば、保持時間が1分以上であっても他の熱処理である。他の熱処理は、処理後に褐変(着色)が生じ難い。
例えば、他の熱処理として、加熱温度90℃以上かつ保持時間1分間未満の加熱殺菌処理を行ってもよい。
In the present embodiment, in addition to performing the heat treatment once or more on the raw material liquid before adding the lactic acid bacteria, heat treatment other than the heat treatment may be performed once or more, if necessary. The other heat treatment may be before or after the heat treatment.
For example, the heating step in which the holding time is less than 1 minute even when the heating temperature is 80 ° C. or higher is another heat treatment. If the heating temperature is less than 80 ° C., another heat treatment is performed even if the holding time is 1 minute or more. Other heat treatments are less likely to cause browning (coloring) after treatment.
For example, as another heat treatment, a heat sterilization treatment having a heating temperature of 90 ° C. or higher and a holding time of less than 1 minute may be performed.
前記熱処理、及び必要に応じた他の熱処理を終えた原料液は、次の発酵工程における発酵温度まで冷却することが好ましい。または熱処理後又は他の熱処理後に、タンク等に保存する場合は10℃以下に冷却することが好ましい。 It is preferable that the raw material liquid after the heat treatment and other heat treatments if necessary are cooled to the fermentation temperature in the next fermentation step. Alternatively, when it is stored in a tank or the like after heat treatment or other heat treatment, it is preferably cooled to 10 ° C. or lower.
[発酵]
熱処理した原料液に乳酸菌を添加し、所定の発酵温度に保持して発酵させ、発酵乳を得る。発酵によりカードが形成される。
乳酸菌は、発酵乳の製造において公知の乳酸菌を使用できる。例えば、ラクトバチルス・ブルガリクス(L.bulgaricus)、ラクトコッカス・ラクチス(L.lactis)、ストレプトコッカス・サーモフィラス(S.thermophilus)等の、発酵乳の製造に通常用いられている乳酸菌スターターの1種または2種以上を用いることが好ましい。
乳酸菌の添加量は、通常の範囲で適宜調節できる。
[fermentation]
Lactic acid bacteria are added to the heat-treated raw material solution and fermented at a predetermined fermentation temperature to obtain fermented milk. Fermentation forms curds.
As the lactic acid bacterium, a known lactic acid bacterium can be used in the production of fermented milk. For example, one of the lactic acid bacteria starters commonly used in the production of fermented milk, such as Lactobacillus bulgaricus, Lactococcus lactis, S. thermophilus, or It is preferable to use two or more kinds.
The amount of lactic acid bacteria added can be appropriately adjusted within a normal range.
乳酸菌による発酵においては酸が生成されるため、発酵が開始された後の原料液のpHは経時的に低下する。発酵工程における到達pHは4.2〜4.8が好ましい。
発酵温度は、例えば35〜43℃が好ましい。発酵時間は7時間以下が好ましく、6時間以下がより好ましい。発酵時間が7時間を超えると製造効率の点で好ましくない。
pHが目標の値(到達pH)に達したら、10℃以下に冷却して発酵乳を得る。10℃以下に冷却された時点を発酵工程の終了時とする。
Since acid is produced in fermentation with lactic acid bacteria, the pH of the raw material liquid after fermentation is started decreases with time. The ultimate pH in the fermentation step is preferably 4.2 to 4.8.
The fermentation temperature is preferably, for example, 35 to 43 ° C. The fermentation time is preferably 7 hours or less, more preferably 6 hours or less. If the fermentation time exceeds 7 hours, it is not preferable in terms of production efficiency.
When the pH reaches the target value (reached pH), the mixture is cooled to 10 ° C. or lower to obtain fermented milk. The time when the temperature is cooled to 10 ° C. or lower is defined as the end of the fermentation process.
静置型発酵乳を製造する場合は、原料液に乳酸菌を添加した後、製品容器(飲食用の容器)に充填して発酵させる。
撹拌型発酵乳を製造する場合は、原料液に乳酸菌を添加した後、例えばタンク内で発酵させ、生じたカードを撹拌して粉砕し、必要に応じて、安定剤の溶液やカード以外の食品材料と混合し、製品容器(飲食用の容器)に充填する。発酵乳以外の食品材料としては、果肉、ソース、ゼリー等が例示できる。撹拌型発酵乳はスプーン等ですくって食べる固形タイプでもよく、ドリンクタイプでもよい。
When producing static fermented milk, lactic acid bacteria are added to the raw material liquid and then filled in a product container (food and drink container) for fermentation.
When producing agitated fermented milk, after adding lactic acid bacteria to the raw material liquid, for example, ferment it in a tank, stir and crush the resulting curd, and if necessary, a stabilizer solution or food other than curd. Mix with ingredients and fill product containers (food and drink containers). Examples of food materials other than fermented milk include pulp, sauce, and jelly. The agitated fermented milk may be a solid type that is scooped up with a spoon or the like and eaten, or a drink type.
本実施形態の発酵乳の製造方法は、静置型発酵乳であっても、撹拌型発酵乳であっても、難消化性デキストリンを含みながら、離水が少ない発酵乳を製造できる。離水が少ないと、保存中の外観や風味の変化が抑えられる。 The method for producing fermented milk of the present embodiment can produce fermented milk containing indigestible dextrin and having little water separation, regardless of whether it is static fermented milk or stirring fermented milk. With less water separation, changes in appearance and flavor during storage are suppressed.
以下に実施例を用いて本発明をさらに詳しく説明するが、本発明はこれら実施例に限定されるものではない。
<測定方法>
原料液のb*値は、色差計(製品名:カラーリーダーCR−13、コニカミノルタジャパン株式会社製)を用いて測定した。具体的には、原料液を10℃に調温後、遮光カップに充填し、カラーリーダーの測定部を垂直にあてて色度を測定した。
Hereinafter, the present invention will be described in more detail with reference to Examples, but the present invention is not limited to these Examples.
<Measurement method>
The b * value of the raw material solution was measured using a color difference meter (product name: Color Reader CR-13, manufactured by Konica Minolta Japan Co., Ltd.). Specifically, after adjusting the temperature of the raw material liquid to 10 ° C., it was filled in a light-shielding cup, and the measuring part of the color reader was applied vertically to measure the chromaticity.
<原料>
脱脂濃縮乳:森永乳業社製。
クリーム:森永乳業社製。
カゼイン加水分解物:下記調製例1で得たカゼイン加水分解物。
難消化性デキストリン:松谷化学工業社製品名 ファイバーソル2。
スクラロース:三栄源エフエフアイ社製。
希少糖含有シロップ:松谷化学工業社製品名 レアシュガースウィート、D−フラクトース31質量%、D−グルコース44質量%、D−プシコース6質量%、及びD−アロース質4質量%を含む。
ラクチュロース含有シロップ:森永乳業社製品名 ミルクオリゴ糖MLS(R)−50、ラクチュロース50質量%を含む。
寒天:三栄源エフエフアイ社製品名 寒天UP−6。
香料:ヨーグルトフレーバー、三栄源エフエフアイ社製。
乳酸菌スターター:DANISCO社製品名 YO−MIX(R)、ストレプトコッカス・サーモフィラス(S.thermophilus)とラクトバチルス・デルブルッキー・サブスピーシーズ・ブルガリカス(L. delbrueckii subsp. bulgaricus)の混合培養物。
ペクチン:三栄源エフエフアイ社製品名 SM−MN−2779。
<Raw materials>
Skim milk concentrate: Made by Morinaga Milk Industry Co., Ltd.
Cream: Made by Morinaga Milk Industry Co., Ltd.
Casein hydrolyzate: The casein hydrolyzate obtained in Preparation Example 1 below.
Indigestible dextrin: Matsutani Chemical Industry Co., Ltd. Product name Fiber Sol 2.
Sucralose: Made by Saneigen FFI.
Rare sugar-containing syrup: Matsutani Chemical Industry Co., Ltd. Product name Contains rare sugar sweet, 31% by mass of D-fructose, 44% by mass of D-glucose, 6% by mass of D-psicose, and 4% by mass of D-allose.
Syrup containing lactulose: Morinaga Milk Industry Co., Ltd. Product name Contains milk oligosaccharide MLS (R) -50 and 50% by mass of lactulose.
Agar: Saneigen FFI Co., Ltd. Product name Agar UP-6.
Fragrance: Yogurt flavor, manufactured by Saneigen FFI.
Lactic Acid Bacteria Starter: YO-MIX (R), a product name of Danisco, a mixed culture of S. thermophilus and L. delbrueckii subspecies bulgaricus.
Pectin: Saneigen FFI Co., Ltd. Product name SM-MN-2779.
[調製例1]
市販のカゼイン(牛乳由来、ニュージーランドデーリーボード社製)100mgに水900mgを加え、よく分散させ、水酸化ナトリウムを添加して溶液のpHを7.0に調整し、カゼインを完全に溶解し、濃度約10%のカゼイン水溶液を調製した。得られたカゼイン水溶液を85℃で10分間加熱殺菌し、50℃に温度調整し、水酸化ナトリウムを添加してpHを9.0に調整した後、パンクレアチン2mg(天野エンザイム社製)、プロテアーゼA4mg(天野エンザイム社製)を添加して、加水分解反応を開始した。8時間後に80℃で6分間加熱して酵素を失活させて酵素反応を停止し、10℃に冷却した。
得られた加水分解液を分画分子量1000の限外ろ過膜(日本ポール社製)で限外ろ過し、濃縮後凍結乾燥し、カゼイン加水分解物を85mg得た。
[Preparation Example 1]
Add 900 mg of water to 100 mg of commercially available casein (derived from milk, manufactured by New Zealand Daily Board), disperse well, add sodium hydroxide to adjust the pH of the solution to 7.0, completely dissolve the casein, and concentrate. About 10% casein aqueous solution was prepared. The obtained casein aqueous solution was sterilized by heating at 85 ° C. for 10 minutes, the temperature was adjusted to 50 ° C., sodium hydroxide was added to adjust the pH to 9.0, and then pancreatin 2 mg (manufactured by Amano Enzyme) and protease. A4 mg (manufactured by Amano Enzyme) was added to initiate a hydrolysis reaction. After 8 hours, the mixture was heated at 80 ° C. for 6 minutes to inactivate the enzyme, stop the enzyme reaction, and cool to 10 ° C.
The obtained hydrolyzate was ultrafiltered with an ultrafiltration membrane (manufactured by Nippon Paul Co., Ltd.) having a molecular weight cut off of 1000, concentrated and then freeze-dried to obtain 85 mg of casein hydrolyzate.
[試験例1〜4]
乳原料と難消化性デキストリンを含む原料液を熱処理したときの、発酵乳の褐変(着色)及び離水の原因を調べるために、表1に示す配合で、固形状の発酵乳(静置型ヨーグルト)を製造した。
試験例1、2は難消化性デキストリンを配合した例、試験例3、4は難消化性デキストリンを配合しない例である。
[Test Examples 1 to 4]
In order to investigate the causes of browning (coloring) and water separation of fermented milk when the raw material liquid containing dairy raw material and indigestible dextrin is heat-treated, solid fermented milk (stationary yogurt) with the formulation shown in Table 1 Manufactured.
Test Examples 1 and 2 are examples containing indigestible dextrin, and Test Examples 3 and 4 are examples not containing indigestible dextrin.
まず、乳酸菌スターター以外の原料をミキサーで混合し、原料液(70℃)を調製した。次いで、ホモジナイザーにより加温及び均質処理(温度85℃、2秒、圧力20MPa)した。均質化処理後の原料液(85℃)を、90℃で10分間の条件で加熱殺菌処理した後、42℃に冷却した。
冷却後の原料液に乳酸菌スターターを添加した直後に容器に充填し、40℃に保持して発酵させた。pHが4.6に達したら10℃以下に冷却して発酵を終了させた。
乳酸菌スターターを添加してから、発酵終了までの時間を発酵時間とする(以下、同様)。各例の発酵時間を表1に示す。
得られた静置型ヨーグルトを、10℃で3日間保管した後、目視で観察し、褐変の有無と離水の有無を調べた。結果を表1に示す。
First, raw materials other than the lactic acid bacterium starter were mixed with a mixer to prepare a raw material liquid (70 ° C.). Then, it was heated and homogenized with a homogenizer (temperature 85 ° C., 2 seconds, pressure 20 MPa). The homogenized raw material solution (85 ° C.) was heat sterilized at 90 ° C. for 10 minutes and then cooled to 42 ° C.
Immediately after adding the lactic acid bacterium starter to the raw material liquid after cooling, the container was filled and kept at 40 ° C. for fermentation. When the pH reached 4.6, the temperature was cooled to 10 ° C. or lower to terminate the fermentation.
The time from the addition of the lactic acid bacterium starter to the end of fermentation is defined as the fermentation time (hereinafter, the same applies). The fermentation time of each example is shown in Table 1.
The obtained static yogurt was stored at 10 ° C. for 3 days and then visually observed to check for browning and water separation. The results are shown in Table 1.
表1の結果に示されるように、試験例1、2は試験例3、4に比べて発酵時間が長く、発酵乳は明らかに褐変していた。また、試験例1、2では容器内壁と固形状の発酵乳との間に隙間があり、離水が顕著に発生した。
試験例1〜4の結果より、難消化性デキストリンが発酵遅延や離水の原因になっていることを知見した。
As shown in the results of Table 1, the fermentation time of Test Examples 1 and 2 was longer than that of Test Examples 3 and 4, and the fermented milk was clearly browned. Further, in Test Examples 1 and 2, there was a gap between the inner wall of the container and the solid fermented milk, and water separation occurred remarkably.
From the results of Test Examples 1 to 4, it was found that indigestible dextrin causes fermentation delay and water separation.
[例1〜11]
表2に示す配合で、試験例1と同じ手順で静置型ヨーグルトを製造した。ただし加熱殺菌処理の条件を表3に示す通りに変更した。
また、均質化処理後、加熱殺菌処理する前の原料液(85℃を10℃に調温したもの)のb*値(b1)、及び乳酸菌スターター添加時の原料液(40℃を10℃に調温したもの℃)のb*値(b2)を測定し、b2/b1の比を算出した。結果を表3に示す。
[Examples 1 to 11]
With the formulation shown in Table 2, a stationary yogurt was produced by the same procedure as in Test Example 1. However, the conditions of heat sterilization treatment were changed as shown in Table 3.
In addition, the b * value (b1) of the raw material liquid (85 ° C. adjusted to 10 ° C.) after the homogenization treatment and before the heat sterilization treatment, and the raw material liquid (40 ° C. to 10 ° C.) when the lactic acid bacterium starter was added. The b * value (b2) of the temperature-controlled temperature) was measured, and the ratio of b2 / b1 was calculated. The results are shown in Table 3.
評価
(1)発酵遅延抑制性
発酵時間を表3に示す。下記の基準で発酵遅延抑制性を評価した。
〇:発酵時間が7時間以下。
×:発酵時間が7時間超。
(2)離水抑制性
得られた静置型ヨーグルトを、10℃で3日間保管した後、容器開口部内を上方から目視で観察し、容器内壁と固形状の発酵乳との間に隙間が生じている部分の、周方向における長さRの合計を測定した。容器開口部の全周に対して、前記周方向の長さRの合計の割合(単位:%)を算出し、壁面剥がれ度とした。下記の基準で離水抑制性を評価した。結果を表3に示す。
◎:壁面剥がれ度が0%。
〇:壁面剥がれ度が0%超、20%以下。
×:壁面剥がれ度が20%超。
Evaluation (1) Fermentation delay inhibitory fermentation time is shown in Table 3. The fermentation delay inhibitory property was evaluated according to the following criteria.
〇: Fermentation time is 7 hours or less.
X: Fermentation time is over 7 hours.
(2) Inhibitory of water separation After storing the obtained static yogurt at 10 ° C. for 3 days, the inside of the container opening was visually observed from above, and a gap was formed between the inner wall of the container and the solid fermented milk. The total length R of the portion in the circumferential direction was measured. The ratio (unit:%) of the total length R in the circumferential direction to the entire circumference of the container opening was calculated and used as the degree of wall peeling. The water separation inhibitory property was evaluated according to the following criteria. The results are shown in Table 3.
⊚: The degree of wall peeling is 0%.
〇: The degree of wall peeling is more than 0% and less than 20%.
X: The degree of wall peeling is over 20%.
[例12、13]
加熱処理条件を変えて静置型ヨーグルトを製造した。
表2に示す配合で、乳酸菌スターター以外の原料をミキサーで混合し、原料液(70℃)を調製した。次いで、ホモジナイザーにより加温及び均質処理(温度80℃、2秒、圧力20MPa)した。
例12では、均質化処理後の原料液(80℃)を、97℃で17分間の条件で加熱殺菌処理(1)を行った後、10℃に冷却した。次いで、90℃で10秒間の条件で加熱殺菌処理(2)を行った後、40℃に冷却した。
例13では、均質化処理後の原料液(80℃)を、96℃で5分間の条件で加熱殺菌処理(1)を行った後、10℃に冷却した。次いで、90℃で10秒間の条件で加熱殺菌処理(2)を行った後、40℃に冷却した。
例12、13において、冷却後の原料液に乳酸菌スターターを添加した後、容器に充填し、40℃に保持して発酵させた。pHが4.6に達したら10℃以下に冷却して発酵を終了させた。
各例の発酵時間を表4に示す。上記の方法で発酵遅延抑制性及び離水抑制性を評価した。結果を表4に示す。
[Examples 12 and 13]
A stationary yogurt was produced by changing the heat treatment conditions.
Raw materials other than the lactic acid bacterium starter were mixed with a mixer according to the formulation shown in Table 2 to prepare a raw material solution (70 ° C.). Then, it was heated and homogenized by a homogenizer (temperature 80 ° C., 2 seconds, pressure 20 MPa).
In Example 12, the raw material liquid (80 ° C.) after the homogenization treatment was subjected to heat sterilization treatment (1) at 97 ° C. for 17 minutes and then cooled to 10 ° C. Then, heat sterilization treatment (2) was performed at 90 ° C. for 10 seconds, and then the mixture was cooled to 40 ° C.
In Example 13, the raw material liquid (80 ° C.) after the homogenization treatment was subjected to heat sterilization treatment (1) at 96 ° C. for 5 minutes and then cooled to 10 ° C. Then, heat sterilization treatment (2) was performed at 90 ° C. for 10 seconds, and then the mixture was cooled to 40 ° C.
In Examples 12 and 13, a lactic acid bacterium starter was added to the cooled raw material solution, filled in a container, and kept at 40 ° C. for fermentation. When the pH reached 4.6, the temperature was cooled to 10 ° C. or lower to terminate the fermentation.
The fermentation time of each example is shown in Table 4. The fermentation delay inhibitory property and water separation inhibitory property were evaluated by the above method. The results are shown in Table 4.
[例14、15]
加熱処理条件を変えて静置型ヨーグルトを製造した。
表2に示す配合で、乳酸菌スターター以外の原料をミキサーで混合し、原料液(70℃)を調製した。次いで、ホモジナイザーにより加温及び均質処理(温度80℃、2秒、圧力20MPa)した。
例14では、均質化処理後の原料液(80℃)を、85℃で6分間の条件で加熱殺菌処理(1)を行った後、10℃に冷却した。次いで、90℃で5秒間の条件で加熱殺菌処理(2)を行った後、40℃に冷却した。
例15では、均質化処理後の原料液(80℃)を、85℃で6分間の条件で加熱殺菌処理(1)を行った後、5℃に冷却した。次いで、85℃で60分間の条件で加熱殺菌処理(2)を行った後、40℃に冷却した。すなわち、85℃以上1分間以上の熱処理を2回行った。
例14、15において、冷却後の原料液に乳酸菌スターターを添加した後、容器に充填し、40℃に保持して発酵させた。pHが4.6に達したら10℃以下に冷却して発酵を終了させた。
各例の発酵時間を表4に示す。上記の方法で発酵遅延抑制性及び離水抑制性を評価した。結果を表4に示す。
[Examples 14 and 15]
A stationary yogurt was produced by changing the heat treatment conditions.
Raw materials other than the lactic acid bacterium starter were mixed with a mixer according to the formulation shown in Table 2 to prepare a raw material solution (70 ° C.). Then, it was heated and homogenized by a homogenizer (temperature 80 ° C., 2 seconds, pressure 20 MPa).
In Example 14, the homogenized raw material solution (80 ° C.) was heat-sterilized (1) at 85 ° C. for 6 minutes and then cooled to 10 ° C. Next, heat sterilization treatment (2) was performed at 90 ° C. for 5 seconds, and then the mixture was cooled to 40 ° C.
In Example 15, the raw material liquid (80 ° C.) after the homogenization treatment was subjected to heat sterilization treatment (1) at 85 ° C. for 6 minutes and then cooled to 5 ° C. Next, heat sterilization treatment (2) was performed at 85 ° C. for 60 minutes, and then the mixture was cooled to 40 ° C. That is, the heat treatment at 85 ° C. or higher for 1 minute or longer was performed twice.
In Examples 14 and 15, a lactic acid bacterium starter was added to the cooled raw material solution, filled in a container, and kept at 40 ° C. for fermentation. When the pH reached 4.6, the temperature was cooled to 10 ° C. or lower to terminate the fermentation.
The fermentation time of each example is shown in Table 4. The fermentation delay inhibitory property and water separation inhibitory property were evaluated by the above method. The results are shown in Table 4.
[例16]
本例では、撹拌型の発酵乳(ドリンクヨーグルト)を製造した。
表5に示す原料液の配合で、乳酸菌スターター以外の原料をミキサーで混合し、原料液(70℃)を調製した。次いで、ホモジナイザーにより加温及び均質処理(温度85℃、2秒で圧力20MPa)した。均質化処理後の原料液(85℃)を、80℃で6分間の条件で加熱殺菌処理(1)を行った後、次いで、121℃で2秒間の条件で加熱殺菌処理(2)を行った後、40℃に冷却した。
冷却後の原料液に乳酸菌スターターを添加した後、タンク内で40℃に保持してpHが4.6に達するまで発酵させ、5℃に冷却して発酵を終了させた。この後、予め調製したペクチン溶液(5℃)を添加し、撹拌して発酵乳を破砕しながら混合した。破砕した発酵乳とペクチン溶液の混合物を容器に充填してドリンクヨーグルトを得た。
本例の発酵時間、及び上記の基準で発酵遅延抑制性を評価した結果を、表6に示す。
[Example 16]
In this example, agitated fermented milk (drink yogurt) was produced.
With the composition of the raw material liquid shown in Table 5, raw materials other than the lactic acid bacterium starter were mixed with a mixer to prepare a raw material liquid (70 ° C.). Then, it was heated and homogenized by a homogenizer (temperature 85 ° C., pressure 20 MPa at 2 seconds). The homogenized raw material solution (85 ° C.) is heat sterilized (1) at 80 ° C. for 6 minutes, and then heat sterilized (2) at 121 ° C. for 2 seconds. After that, it was cooled to 40 ° C.
After adding a lactic acid bacterium starter to the cooled raw material solution, the mixture was kept at 40 ° C. in a tank and fermented until the pH reached 4.6, and cooled to 5 ° C. to complete the fermentation. Then, a pre-prepared pectin solution (5 ° C.) was added, and the mixture was stirred and mixed while crushing the fermented milk. A mixture of crushed fermented milk and a pectin solution was filled in a container to obtain a drink yogurt.
Table 6 shows the fermentation time of this example and the results of evaluating the fermentation delay inhibitory property based on the above criteria.
得られたドリンクヨーグルトを、10℃で8日間静置した後、容器内のヨーグルトに発生した離水(上澄み部分)の質量を測定した。ドリンクヨーグルトの総質量に対する、離水の割合(単位:質量%)を離水発生量とした。下記の基準で離水抑制性を評価した。結果を表6に示す。
〇:離水発生量が20質量%以下。
×:離水発生量が20質量%超。
The obtained drink yogurt was allowed to stand at 10 ° C. for 8 days, and then the mass of water separation (supernatant portion) generated in the yogurt in the container was measured. The ratio of water separation (unit: mass%) to the total mass of drink yogurt was defined as the amount of water separation generated. The water separation inhibitory property was evaluated according to the following criteria. The results are shown in Table 6.
〇: The amount of water separation generated is 20% by mass or less.
X: The amount of water separation generated exceeds 20% by mass.
表3、4、6の結果に示されるように、b2/b1の比が1.95以下であると、難消化性デキストリンを含みながら、離水が生じ難い発酵乳が得られた。 As shown in the results of Tables 3, 4 and 6, when the ratio of b2 / b1 was 1.95 or less, fermented milk containing indigestible dextrin but less likely to cause water separation was obtained.
Claims (5)
前記熱処理を施す前の前記原料液の、CIE L*a*b*表色系におけるb*値をb1、前記乳酸菌添加時の前記原料液の前記b*値をb2とすると、b2/b1の比が1.95以下となるように前記熱処理を行う、発酵乳の製造方法。 A method of obtaining fermented milk by subjecting a raw material solution containing a dairy raw material and indigestible dextrin to a heat treatment at a heating temperature of 80 ° C. or higher and a holding time of 1 minute or longer, and then adding lactic acid bacteria and fermenting the milk.
Assuming that the b * value of the raw material liquid before the heat treatment is b1 in the CIE L * a * b * color system and the b * value of the raw material liquid at the time of adding the lactic acid bacteria is b2, b2 / b1 A method for producing fermented milk, wherein the heat treatment is performed so that the ratio is 1.95 or less.
前記熱処理を施す前の前記原料液の、CIE L*a*b*表色系におけるb*値をb1、前記乳酸菌添加時の前記原料液の前記b*値をb2とすると、b2/b1の比が1.95以下となるように前記熱処理を行う、発酵乳の離水の抑制方法。 This is a method of suppressing water separation of fermented milk fermented by adding lactic acid bacteria after heat treatment of a raw material solution containing a dairy raw material and indigestible dextrin at a heating temperature of 80 ° C. or higher and a holding time of 1 minute or longer. ,
Assuming that the b * value of the raw material liquid before the heat treatment is b1 in the CIE L * a * b * color system and the b * value of the raw material liquid at the time of adding the lactic acid bacteria is b2, b2 / b1 A method for suppressing water separation of fermented milk, wherein the heat treatment is performed so that the ratio is 1.95 or less.
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Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH05344864A (en) * | 1990-09-20 | 1993-12-27 | Morinaga Milk Ind Co Ltd | Functional food effective in improving lipid metabolism |
JP2009089625A (en) * | 2007-10-05 | 2009-04-30 | Asahi Kasei Chemicals Corp | Fermented milk containing indigestible oligosaccharide and lactobacillus |
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Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH05344864A (en) * | 1990-09-20 | 1993-12-27 | Morinaga Milk Ind Co Ltd | Functional food effective in improving lipid metabolism |
JP2009089625A (en) * | 2007-10-05 | 2009-04-30 | Asahi Kasei Chemicals Corp | Fermented milk containing indigestible oligosaccharide and lactobacillus |
Non-Patent Citations (1)
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"ヨーグルトでは初! 1つの商品で3つの機能性表示 「トリプルヨーグルト ドリンクタイプ/トリプルヨーグ", NEWS RELEASE, JPN6023018365, February 2019 (2019-02-01), pages 1 - 3, ISSN: 0005051420 * |
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