JP2020180102A - Anti-norovirus agent, disinfectant and cleaning agent - Google Patents

Abstract

To provide an anti-norovirus agent that has an excellent anti-norovirus effect and is light in color and does not easily spoil the appearance of the object.SOLUTION: Provided is an anti-norovirus agent containing an extract of a linden plant by 0.005 mass% or more and 0.17 mass% or less. Said anti-norovirus agent contains a predetermined amount of an extract of a linden plant, and, as a result, exhibits an excellent anti-norovirus effect and achieves an excellent effect that it is light in color and does not easily impair the appearance of the object.SELECTED DRAWING: None

Description

The present invention relates to anti-norovirus agents, disinfectants and cleaning agents.

In recent years, outbreaks of infectious gastroenteritis and food poisoning due to norovirus have occurred frequently throughout the year, and the peak of the outbreak is from November to March. Norovirus is an RNA virus that does not have an envelope and is classified into the Caliciviridae and Norovirus genus, and has strong resistance to alcohol (ethanol, isopropanol, etc.), inverted soap, heat, acidity (stomach acid, etc.), and dryness. Has. The incubation period of norovirus is thought to be 1 to 2 days, and the main symptoms of vomiting, vomiting, and diarrhea appear, but it may also be accompanied by abdominal pain, headache, fever, chills, myalgia, sore throat, and malaise. is there.

Oral infection is known as one of the infection routes of norovirus, and infection is established by ingesting food or water contaminated with norovirus. Therefore, in places such as restaurants, school lunch facilities, and factories where food is cooked and processed, it is required to prevent food, water, equipment, etc. from being contaminated with norovirus.

Norovirus has a high infectivity so that it can be developed even by ingesting a small amount of about 10 to 100. Therefore, an anti-norovirus agent that acts directly on norovirus and reduces or eliminates the infectious ability of norovirus is desired.

According to the Ministry of Health, Labor and Welfare, in order to inactivate norovirus, it is recommended to heat cooking utensils at 85 ° C for 1 minute and disinfect with 200 ppm sodium hypochlorite (see Non-Patent Document 1). .. However, the inactivation of norovirus by heating has a problem of heat resistance of the object, and sodium hypochlorite has strong metal corrosiveness, which may limit its use. Furthermore, it is assumed that sodium hypochlorite will be decomposed in places with a lot of organic matter such as kitchens, and sufficient effects will not be obtained.

Furthermore, sodium hypochlorite has a risk of invading the skin and mucous membranes, so it is difficult to apply it to the human body. Furthermore, it reacts with acid to generate toxic gas, so care must be taken when handling and using it. is there. Under such circumstances, there is a strong demand for an anti-norovirus agent that is easy to handle and can exert a sufficient inactivating effect even when sodium hypochlorite cannot be used.

As a method for safely inactivating norovirus and the like to the human body, a method using a food-derived virus inactivating substance is being studied.

For example, Patent Document 1 shows that an extract of a plant of the genus Persimmon containing tannin is used as a composition mixed with ethanol, and norovirus and the like can be inactivated by using this composition.

Further, for example, Patent Document 2 describes a norovirus agent containing a predetermined amount of proanthocyanidin, which is a grape seed extract.

International Publication No. 2008/153077 Japanese Unexamined Patent Publication No. 2013-47196

Ministry of Health, Labor and Welfare website "Q & A for norovirus"; https://www.mhlw.go.jp/stf/seisakunitsuite/bunya/kenkou_iryou/shokuhin/syokuchu/kanren/yobou/040204-1.html#04

As described above, Patent Document 1 shows that norovirus and the like can be inactivated by a composition containing an extract of a plant of the genus Persimmon containing tannin and ethanol. However, this composition has a brown color and has a problem of impairing the appearance of the object to be disinfected.

The norovirus agent of Patent Document 2 has an excellent norovirus effect. However, even this norovirus killing agent has a brown color depending on the mode of use, and when applied to a white or transparent object to be disinfected (for example, tableware, cooking utensils, processing equipment, conveyor, cloth, etc.), it is subject to disinfection. There is a problem that the appearance of the object may be spoiled, and further improvement is required.

Under such circumstances, it is a main object of the present invention to provide an anti-norovirus agent which has an excellent anti-norovirus effect and is light in color and does not easily impair the appearance of the object. Another object of the present invention is to provide a disinfectant and a cleaning agent using the anti-norovirus agent.

The present inventors have made diligent studies to solve the above problems. As a result, it was found that by using an extract of a linden plant as an anti-norovirus agent, an excellent anti-norovirus effect is exhibited, and the color is light and the appearance of the object is not easily spoiled. Furthermore, it has been found that the anti-norovirus agent is also excellent in storage stability in a high temperature environment. The present invention has been completed by further studies based on these findings.

That is, the present invention provides the inventions of the following aspects.
Item 1. An anti-norovirus agent containing 0.005% by mass or more and 0.17% by mass or less of an extract of a linden plant.
Item 2. Item 2. The anti-norovirus agent according to Item 1, wherein the extract of the linden plant is contained in an aqueous ethanol solution having a concentration of 30% by mass or more.
Item 3. Item 3. The anti-norovirus agent according to Item 1 or 2, wherein the color difference ΔE _{1 from the} 95% by volume ethanol aqueous solution in the CIE1976 L ^* a ^* b ^* color space is less than 10.00.
Item 4. Item 1 of Items 1 to 3, wherein the color difference ΔE _{2 from the} 95% by volume ethanol aqueous solution in the CIE1976 L ^* a ^* b ^* color space is less than 15.00 after storage in an environment of 40 ° C. for 30 days. The anti-norovirus agent described in.
Item 5. In CIE1976 L ^* a ^* b ^* color space, a color change Delta] E ₁₂ in before and after storing for 30 days at 40 ° C. environment is less than 5.00, anti-norovirus agent according to any one of claim 1-4 ..
Item 6. Any one of Items 1 to 5, wherein the linden plant is at least one selected from the group consisting of Bodaiju, Tilia cordata, Tilia platyphylla, Tilia platyphylla, American bodaiju, Tilia maximowicz, Manshubodaiju, and Tsukushibodaiju. The anti-norovirus agent described in the section.
Item 7. A disinfectant containing the anti-norovirus agent according to any one of Items 1 to 6.
Item 8. A cleaning agent containing the anti-norovirus agent according to any one of Items 1 to 6.

According to the present invention, it is possible to provide an anti-norovirus agent that exhibits an excellent anti-norovirus effect and is light in color and does not easily impair the appearance of the object. Furthermore, according to the present invention, it is also possible to provide a disinfectant and a cleaning agent using the anti-norovirus agent.

It is a graph which shows the relationship between the elapsed days (30 days) that the anti-norovirus agent was stored in the environment of 40 degreeC, and the color difference ΔE.

Hereinafter, the anti-norovirus agent of the present invention, and the disinfectant and cleaning agent using the same will be described in detail. In this specification, the numerical values connected by "-" mean a numerical range including the numerical values before and after "-" as the lower limit value and the upper limit value. When a plurality of lower limit values and a plurality of upper limit values are described separately, any lower limit value and upper limit value can be selected and connected by "~".

The anti-norovirus agent of the present invention is characterized by containing an extract of a linden plant in an amount of 0.005% by mass or more and 0.17% by mass or less. The anti-norovirus agent of the present invention exhibits an excellent anti-norovirus effect by containing a predetermined amount of an extract of a plant of the genus Lindens, and also exhibits an excellent effect of being light in color and not spoiling the appearance of the object. ..

In the anti-norovirus agent of the present invention, an extract of a linden plant is contained as an active ingredient. In the anti-norovirus agent of the present invention, the extract of the linden plant is derived from the linden plant, has a high inactivating effect on the norovirus, and can safely inactivate the norovirus to the human body.

Among the plants of the genus Tilia miqueliana, Bodaiju (Tilia miqueliana Maxim.), Fuyubodaiju (Tiliacordata Mill., Tiliaulmifolia Scop., Tiliaparvifolia Ehrh.), Natsubodaiju (also known as Tilia platyphyllos Scop.), Seiyo Shinanoki ((also known as Linden) Tilia x europaea L.), American bodaiju (Tilia americana L.), Oobabodaiju (Tilia maximowicziana Shiras.), Manshubodaiju (Tilia mandshurica Rupr. Et Maxim. Var. Mandshurica), Tsukushibodaiju ( Tilia mandshurica Rupr. Et Maxim. Var. Rufovillosa (Hatus.) Kitam.) Is preferred, Bodaiju, Fuyubodaiju, Natsubodaiju, and Tilia cordata are more preferred, and Fuyubodaiju is particularly preferred. As the extract of the linden plant, only one kind may be used, or two or more kinds may be mixed and used.

The extraction site of the linden plant is not particularly limited as long as the effect of the present invention is exhibited, and for example, the above-ground part such as the leaf part, the branch part, the stem part, the flower part, the bud part, the fruit part, and the pericarp part. A part or a mixture thereof is mentioned, and among these, a flower part is preferable. The extract of the linden plant preferably contains the extract of the flower part of Tilia cordata.

As a method for preparing the extraction site of the linden plant, it can be obtained as it is or by pulverizing it with a coarse crusher and subjecting it to solvent extraction.

Extracts of linden plants can be easily obtained by utilizing commonly used methods for extracting plants. Moreover, you may use the commercial product as the extract of the linden plant. The extract of the linden plant includes an extract of the linden plant, a diluted solution or a concentrated solution of the extract, a dried product of the extract, or a crude or purified product thereof. Is done.

The extraction solvent of the extract of the linden plant is not particularly limited and may be appropriately selected depending on the intended purpose. Examples thereof include water, a hydrophilic solvent, and a mixed solvent thereof. The water is not particularly limited, and for example, pure water, tap water, well water, mineral spring water, mineral water, hot spring water, spring water, fresh water, purified water, hot water, ion exchange water, physiological saline, phosphate buffer solution. , Phosphate buffered saline and the like. The hydrophilic solvent is not particularly limited, and for example, lower alcohols having 1 to 5 carbon atoms such as methanol, ethanol, propyl alcohol and isopropyl alcohol; lower aliphatic ketones such as acetone and methyl ethyl ketone; 1,3-butylene. Examples thereof include polyhydric alcohols having 2 to 5 carbon atoms such as glycol, propylene glycol and glycerin. The mixed solvent of water and the hydrophilic solvent is not particularly limited and may be appropriately selected depending on the intended purpose. However, when a lower alcohol is used, 1 part by mass to 90 parts by mass with respect to 10 parts by mass of water. 1 part by mass to 40 parts by mass with respect to 10 parts by mass of water when using a lower aliphatic ketone, and 1 part by mass to 90 parts by mass with respect to 10 parts by mass of water when using a polyhydric alcohol. , Preferably added. The extraction solvent is preferably used at room temperature or a temperature equal to or lower than the boiling point of the solvent. Among these, water and hydrous ethanol are preferable as the extraction solvent.

The method for extracting the extract of the linden plant is not particularly limited as long as the fat-soluble component contained in the extraction raw material of the linden plant can be eluted into the solvent, and the method may be carried out according to a conventional method. it can. Further, in the extraction process, it is not necessary to adopt a special extraction method, and any device can be used at room temperature or under reflux heating. Specifically, as an extraction method, for example, an extraction raw material of a plant of the genus Shinanoki is put into a treatment tank filled with a solvent such as an aqueous ethanol solution, and the temperature is raised to 80 ° C. with a reflux extractor while appropriately stirring as necessary. Then, the mixture is heat-extracted for 2 hours, filtered at hot time to elute the fat-soluble component, concentrated under reduced pressure using an evaporator, and further subjected to the same filtration treatment. At this time, the extraction conditions can be appropriately adjusted according to the extraction raw material and the like, but the amount of the extraction solvent is preferably 5 to 20 times the amount (mass ratio) of the extraction raw material, and the extraction time is 1. The time is preferably 3 hours, and the extraction temperature is preferably 20 ° C. to 95 ° C.

In addition, in order to obtain a diluted product, a concentrated product, a dried product, a crude product, a purified product, etc. of the obtained extract of the genus Lindens, even if the treatment such as dilution, concentration, drying, and purification is performed according to a conventional method. Good. Further, the obtained extract of the linden plant can be used as it is as an anti-norovirus agent, but the concentrated solution and the dried product are preferable in terms of ease of use. In obtaining the dried product, carriers such as dextrin and cyclodextrin may be added in order to improve hygroscopicity.

The anti-norovirus agent of the present invention may contain a predetermined amount of an extract of a linden plant, and a commercially available product of the extract of a linden plant is easily available. Commercially available products include Maruzen Pharmaceutical Co., Ltd.'s brand name "Bodaiju Extract Powder MF", trade name "linden extract", "linden extract BG-J", and Ichimaru Falcos Co., Ltd.'s brand name "Falcolex Bodaiju". Can be mentioned.

As the components contained in the extract of plants of the genus Lindens, for example, in the case of flower parts, essential oils, mucous polysaccharides, tannins, flavonoids, kaempferol glycosides, phenols and the like are known.

The anti-norovirus agent of the present invention may contain 0.005 to 0.17% by mass of an extract of a linden plant, but contains an extract of a linden plant from the viewpoint of making the anti-norovirus effect and light color suitable. The ratio is preferably 0.008 to 0.17% by mass, and more preferably 0.01 to 0.17% by mass. In the anti-norovirus agent of the present invention, the content of the extract of the linden plant means the concentration of the component derived from the linden plant.

The anti-norovirus agent of the present invention has a color difference ΔE ₁ of less than 10.00 from the 95% by volume ethanol aqueous solution in the CIE1976 L ^* a ^* b ^* color space from the viewpoint of making the anti-norovirus effect and light color suitable. It is preferably present, and particularly preferably less than 5.00. When the color difference ΔE _{1 from the} 95% by volume ethanol aqueous solution is 5.00 ≦ ΔE ₁ <10.00, the color is lightly colored but pale, and the appearance is not easily spoiled even if the object is white or colorless. , ΔE ₁ <5.00, the anti-norovirus agent is extremely pale in color and is recognized as almost colorless and transparent to the naked eye. Therefore, even if the object is white or colorless, there is almost no risk of spoiling the appearance.

Further, the anti-norovirus agent of the present invention has a color difference ΔE ₂ of less than 15.00 from the 95% by volume ethanol aqueous solution in the CIE1976 L ^* a ^* b ^* color space after being stored in an environment of 40 ° C. for 30 days. It is preferably less than 10.00, more preferably less than 5.00, and particularly preferably less than 5.00. If the color difference ΔE _{2 from the} 95% by volume ethanol aqueous solution is less than 15.00 after storage in a high temperature environment of 40 ° C. for 30 days, the storage stability is good, and the color difference ΔE ₂ is 10.00. If it is less than 5.00, the storage stability is excellent, and if the color difference ΔE ₂ is less than 5.00, the storage stability is particularly excellent.

Further, the anti-norovirus agent of the present invention preferably has a color change ΔE ₁₂ of less than 5.00 before and after storage in a CIE1976 L ^* a ^* b ^* color space for 30 days in a 40 ° C. environment.

The norovirus targeted for inactivation by the anti-norovirus agent of the present invention is at least one of human norovirus (HNV), feline calicivirus (FCV), and murine norovirus (MNV), and human norovirus is particularly unsuitable. It can be the target of activation.

The anti-norovirus agent of the present invention preferably further contains at least one of water and a polar organic solvent in addition to the extract of the linden plant.

The polar organic solvent is not particularly limited as long as it does not inhibit the effect of the present invention, but lower alcohols such as ethanol and isopropanol are preferable, and ethanol is more preferable. In addition, the anti-norovirus agent of the present invention preferably contains an aqueous ethanol solution. That is, it is particularly preferable that the anti-norovirus agent of the present invention contains an extract of a linden plant in an aqueous ethanol solution.

For example, when the anti-norovirus agent of the present invention is used as a disinfectant or a cleaning agent, the concentration of ethanol in the aqueous ethanol solution is about 30% by mass or more at the lower limit and about 30% by mass to about 60% by mass in the range. Is the best.

From the viewpoint of enhancing the inactivating action on norovirus, the pH of the anti-norovirus agent of the present invention is preferably in the range of 2.0 to 10.0, and more preferably in the range of 2.5 to 7.0. preferable.

The pH of the anti-norovirus agent of the present invention can be adjusted with a known pH adjusting agent or buffer solution. Inorganic acids such as hydrochloric acid, nitrate, sulfuric acid, and phosphoric acid; acetic acid, malic acid, citric acid, lactic acid, fumaric acid, succinic acid, tartaric acid, gluconic acid, phosphoric acid, benzoic acid, ascorbic acid, erythorbin Organic acids such as acids, sorbic acid, adipic acid, cinnamic acid, itaconic acid, ferulic acid, phytic acid and salts thereof; hydroxides of alkali metals such as sodium hydroxide and potassium hydroxide; potassium carbonate, hydrogen carbonate Examples include carbonates such as sodium. In addition, examples of the buffer solution include a phosphate buffer solution, a citrate phosphate buffer solution, and a lactic acid buffer solution.

In addition, the anti-norovirus agent of the present invention may contain an additive. The additive is not particularly limited, and an additive that can be used in a known antiviral agent can also be used in the present invention. Examples of the additive include antioxidants, amino acids and the like. Only one type of additive may be used, or two or more types may be mixed and used. When the anti-norovirus agent of the present invention contains an additive, its concentration is not particularly limited as long as it does not inhibit the effect of the present invention, and is, for example, about 0.001 to 4.0% by mass. Can be mentioned.

Antioxidants include L-ascorbic acid, L-ascorbic acid palmitate, L-ascorbic acid phosphate magnesium salt, L-ascorbic acid 2-glucoside, sodium L-ascorbic acid, D-ascorbic acid, D- Sodium ascorbate, L-cysteine, N-acetyl L-cysteine, cystine, glutathione (oxidized form), glutathione (reduced form), L-ergothioneine, thioctic acid, ferulic acid, edetate, propyl gallate, tocopherol, dibutyl Hydroxytoluene, butylhydroxyanisole, sodium sulfite, potassium sulfite, potassium pyrosulfite, sulfur dioxide, coffee bean extract (chlorogenic acid), green tea extract (catechin), rosemary extract, yamamomo extract, amla extract, yeast Examples include extracts. By including the antioxidant, it is possible to suppress the coloring of the anti-norovirus agent of the present invention due to oxidation.

Amino acids include L-phenylalanine, L-methionine, L-isoleucine, L-alanine, DL-alanine, L-serine, L-cysteine, L-arginine, L-lysine, L-valine, L-glycine, L- Theanine, L-threonine, L-proline, L-histidine, L-glutamine, L-glutamic acid, L-leucine, L-tryptophane, L-ornithine, L-citrulin, L-tyrosine, L-carnitine and salts thereof, etc. Can be mentioned. By containing amino acids, the pH of the anti-norovirus agent of the present invention can be adjusted, and coloring due to oxidation can be suppressed.

The anti-norovirus agent of the present invention includes general antiviral components such as acyclovir, phenoxyethanol, chlorhexidine gluconate, benzalkonium chloride, benzethonium chloride, cetylpyridinium chloride, alkyldiamino hydrochloride, as long as the characteristics of the present invention are not impaired. It can also contain general antibacterial components such as ethylglycine and isopropylmethylphenol.

Further, even if glycerin fatty acid ester is added to enhance the sterilizing power against general bacteria, it does not affect the inactivating action on norovirus, so there is no problem. Examples of the glycerin fatty acid ester include glycerin monocapric acid ester, glycerin monolauric acid ester, glycerin monomyristic acid ester, glycerin monopalmitic acid ester, glycerin monostearic acid ester, glycerin monoisostearic acid ester, glycerin monooleic acid ester, and glycerin monobehen. Glycerin monofatty acid ester such as acid ester; Glycerin difatty acid ester such as glycerin distearate, glycerin diisostearic acid ester, glycerin dioleic acid ester; acetic acid monoglyceride, citric acid monoglyceride, succinic acid monoglyceride, diacetyl tartrate monoglyceride, lactic acid monoglyceride Examples include monoglyceride derivatives. In the present invention, one or more of these can be selected and used. From the viewpoint of enhancing the sterilizing power against general bacteria, it is preferable to use glycerin monofatty acid ester of fatty acid having 8 to 12 carbon atoms such as glycerin monocaprylic acid ester, glycerin monocapric acid ester, and glycerin monolauric acid ester.

Further, the anti-norovirus agent of the present invention has a surface activity other than the above-mentioned glycerin fatty acid ester for the purpose of imparting detergency and foaming property within a range that does not affect the inactivation of norovirus or the stability of the composition. The agent can be added. Examples of such surfactants include cationic surfactants such as primary to tertiary fatty amine salts, quaternary ammonium salts, and 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salts; Alkyl betaine type such as N, N-dimethyl-N-alkyl-N-carboxymethylammonium betaine, aminocarboxylic acid type such as N, N-dialkylaminoalkylene carboxylic acid, N, N, N-trialkyl-N-sulfo An alkylsulfobetaine type such as alkyleneammonium betaine and an imidazoline type amphoteric surfactant such as 2-alkyl-1-hydroxyethyl-1-carboxymethylimidazolinium betaine can be used. In addition, water-soluble polymers such as arabic rubber, carrageenan, sodium carboxymethyl cellulose, hydroxycellulose, and sodium polyacrylate; sodium ethylenediamine tetraacetate, tripolyphosphate, and hexametallin for the purpose of foam increase, improvement of formulation stability, and flavoring. Metal ion sequestering agents such as acid salts; inorganic salts such as sodium chloride, potassium chloride and sodium sulfate; fragrances and the like may be added.

The anti-norovirus agent of the present invention can be used as a disinfectant or a cleaning agent (bactericidal cleaning agent) as it is or by adding various carriers, other surfactants, additive components such as a disinfectant and the like. That is, the disinfectant and the cleaning agent of the present invention each contain the anti-norovirus agent of the present invention.

The disinfectant or cleaning agent of the present invention can be provided in various forms such as liquid, liquid-impregnated sheet, gel, and powder, but in addition to acting on a wide range of disinfectant objects in a short time. Therefore, it is preferably in the form of a liquid or a sheet impregnated with a liquid. The liquid disinfectant or cleaning agent can be provided as a lotion, a spray agent, etc., and is filled in a bottle with a measuring cap, a trigger type spray container, a squeeze type or a dispenser type pump spray container, etc., and sprayed or sprayed. And so on. The sheet-shaped disinfectant or cleaning agent impregnated with the liquid can be provided as a wet sheet by impregnating paper, cloth or the like with the disinfectant or cleaning agent liquid and filling it in a container such as a bottle or a bucket.

The disinfectant or cleaning agent of the present invention can be used for disinfecting facilities such as hospital rooms, living rooms, kitchens, bathrooms, washrooms, and toilets, sterilizing and cleaning, furniture such as tables, chairs, and bedding, tableware, cooking utensils, and medical supplies. It can be widely used for disinfection, sterilization and cleaning of utensils or equipment, devices, etc., where there is a possibility of norovirus, or for disinfection and sterilization and cleaning of objects that may be contaminated with norovirus.

The disinfectant or cleaning agent of the present invention may be used in an amount such that the surface of the object to be disinfected is evenly wetted. Further, the action time of the disinfectant or cleaning agent of the present invention on the object to be disinfected may be short, and a sufficient disinfection or sterilization cleaning effect can be obtained in 1 minute to 5 minutes.

Hereinafter, the present invention will be described in detail with reference to Examples and Comparative Examples. In addition, "%" of each component in Table 1 means mass% unless otherwise specified. Details of each component used in Examples and Comparative Examples are as follows.

-Bodaiju extract: Bodaiju extract powder MF (Maruzen Pharmaceuticals Co., Ltd.) Since 50% by mass of dextrin is contained, Table 1 shows the concentration of components derived from Bodaiju excluding this.
-Grape seed extract: Gravinol SE (Kikkoman Biochemifa Co., Ltd.)
-Magnesium sulfate: Brand name "magnesium sulfate (anhydrous)" of Fujifilm Wako Pure Chemical Industries, Ltd.

<Examples 1 to 3 and Comparative Examples 1 to 5>
Each component was mixed so as to have the composition shown in Table 1 to prepare an anti-norovirus agent.

<Evaluation of norovirus inactivation>
Using the norovirus agents obtained in Examples 1 to 3 and Comparative Examples 1 to 5, the disappearance of the human norovirus (HNV) gene was confirmed by the following procedure, and the inactivation of norovirus was evaluated. .. The results are shown in Table 1. The effect of the composition of the present invention on norovirus was examined by the RT-PCR method as follows. Since norovirus cannot be cultured in the laboratory, a norovirus sample crudely purified from the feces of a norovirus patient (virus titer is estimated to be about 8.0) is diluted 10-fold with PBS and used as a test norovirus solution. Then, 900 μL of the sample solution and 100 μL of the virus solution were mixed and allowed to act on a rotator for 1 minute. Immediately after that, 140 μL of the working solution was collected, and viral RNA was extracted using the QIAamp® Viral RNA Mini kit manufactured by QIAGEN. CDNA was obtained from the extracted viral RNA using random hexamer and reverse transcriptase. PCR was performed using the obtained cDNA as a template and G2-SKF (CNTGGGAGGGGCGATCGCAA: SEQ ID NO: 3) and G2-SKR (CCRCCNGCATRHCCRTTRACAT: SEQ ID NO: 4) as primers. For the primers, refer to FIG. 3 and Table 11 of Attachment No. 0514004, "Detection Method of Norovirus" issued on May 14, 2007 by Food Safety Inspection. If the norovirus gene can be detected, another primer may be used. The obtained PCR product was electrophoresed and separated by agarose gel electrophoresis to detect viral genes.
〇: The band derived from the human norovirus (HNV) gene has disappeared and has an inactive effect on HNV.
X: The band derived from the human norovirus (HNV) gene has not disappeared and has no inactive effect on HNV.

<Color evaluation>
The chromaticity (CIE1976 L ^* a ^* b ^* color) of each of the norovirus agents obtained in Examples 1 to 3 and Comparative Examples 1 to 5 was used using a color difference meter (Konica Minolta color difference meter CR-5). Space) was measured. Each norovirus agent was sandwiched between two transparent cells (height 45 mm, plate thickness 1.25 mm, width 10 mm), and the measurement was performed under the condition of an optical path length of 10 mm. Table 1 shows the color difference ΔE ₁ from the chromaticity (100, 0, 0) of the 95 mass% ethanol aqueous solution, respectively.

The color difference ΔE ₁ = √ ((L ₀ −L ₁ ) ² + (a ₀ −a ₁ ) ² + (b ₀ −b ₁ ) ² ).

In addition, the evaluation of light color was based on the following evaluation criteria using the color difference ΔE ₁ .
ΔE ₁ <5.00: The anti-norovirus agent is extremely pale in color and is recognized as almost colorless and transparent to the naked eye. Therefore, even if the object is white or colorless, there is almost no risk of spoiling the appearance.
5.00 ≦ ΔE ₁ <10.00: It is lightly colored but light in color, and even if the object is white or colorless, the appearance is not easily spoiled.
10.00 ≦ ΔE ₁ : Since the color is conspicuous, there is a risk of spoiling the appearance of a white or colorless object.

As described above, since the used Bodaiju extract contains 50% by mass of dextrin, Table 1 shows the concentration of the component derived from Bodaiju excluding this.

<Change in color difference in 40 ° C storage environment>
The anti-norovirus agent (80 mL) obtained in Example 2 and Comparative Example 1 was placed in a PP sampler bottle (100 mL) manufactured by Sampler Tech Co., Ltd., and placed in a high temperature incubator (TVN680TA) manufactured by ADVANTEC in an environment of 40 ° C. After storage for 30 days, the color difference ΔE ₂ from the chromaticity (100, 0, 0) of the 95% by mass ethanol aqueous solution was measured in the same manner as in the above <Color evaluation>. The results are shown in Table 2.

The color difference ΔE ₂ = √ ((L ₀ −L ₂ ) ² + (a ₀ −a ₂ ) ² + (b ₀ −b ₂ ) ² ).

In addition, the evaluation of light color was based on the following evaluation criteria using the color difference ΔE ₂ .
ΔE ₂ <5.00: The anti-norovirus agent is extremely pale in color and is recognized as almost colorless and transparent to the naked eye. Therefore, even if the object is white or colorless, there is almost no risk of spoiling the appearance.
5.00 ≦ ΔE ₂ <10.00: It is lightly colored but light in color, and even if the object is white or colorless, the appearance is not easily spoiled.
10.00 ≦ ΔE ₂ <15.00: Since the color is conspicuous, there is a risk of spoiling the appearance of a white or colorless object, but it is within an acceptable range considering that it has been stored in an environment of 40 ° C. for 30 days.
15.00 ≤ ΔE ₂ : The color is intensely noticeable.

Next, the change ΔE ₁₂ of the color difference before and after storage was calculated. The change ΔE _{12 of the} color difference is calculated by the following formula. The results are shown in Table 2. In addition, the graph of FIG. 1 shows the relationship between the number of days elapsed due to storage and the color difference ΔE.
ΔE ₁₂ = √ ((L ₁ −L ₂ ) ² + (a ₁ −a ₂ ) ² + (b ₁ −b ₂ ) ² )

As described above, since the used Bodaiju extract contains 50% by mass of dextrin, Table 2 shows the concentration of components derived from Bodaiju excluding this.

As is clear from the results shown in Tables 1 and 2, the anti-norovirus agent containing the Bodaiju extract, which is a plant of the genus Linden, has an excellent anti-norovirus effect and is pale in color and impairs the appearance of the object. It turns out to be difficult. Furthermore, it can be seen that even when stored in a high temperature environment of 40 ° C. for 30 days, coloring is effectively suppressed and storage stability is also excellent.

Claims (8)

An anti-norovirus agent containing 0.005% by mass or more and 0.17% by mass or less of an extract of a linden plant. The anti-norovirus agent according to claim 1, wherein the extract of the linden plant is contained in an aqueous ethanol solution having a concentration of 30% by mass or more. The anti-norovirus agent according to claim 1 or 2, wherein the color difference ΔE _{1 from the} 95% by volume ethanol aqueous solution in the CIE1976 L ^* a ^* b ^* color space is less than 10.00. Any 1 of claims 1 to 3, wherein the color difference ΔE _{2 from the} 95% by volume ethanol aqueous solution in the CIE1976 L ^* a ^* b ^* color space is less than 15.00 after storage in an environment of 40 ° C. for 30 days. The anti-norovirus agent described in the section. The anti-norovirus according to any one of claims 1 to 4, wherein the color change ΔE ₁₂ before and after storage in the CIE1976 L ^* a ^* b ^* color space for 30 days in a 40 ° C environment is less than 5.00. Agent. Any one of claims 1 to 5, wherein the linden plant is at least one selected from the group consisting of Bodaiju, Tilia cordata, Tilia platyphylla, Tilia platyphylla, American bodaiju, Tilia maximowicz, Manshubodaiju, and Tsukushibodaiju. The anti-norovirus agent according to item 1. A disinfectant containing the anti-norovirus agent according to any one of claims 1 to 6. A cleaning agent containing the anti-norovirus agent according to any one of claims 1 to 6.

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