JP2020171270A - Liquid housing container, liquid housing container set, and aseptic connecting kit - Google Patents

Abstract

To provide a liquid housing container in which the length of a liquid feeding tube formed by an aseptic connecting device can be made constant.SOLUTION: A liquid housing container 1 comprises: a container body 20 which defines a housing space 22 of liquid; a first tubular member 40 whose one end 40a is connected to the container body 20; and a first closing part 48 which closes the first tubular member 40, in which the first tubular member 40 has an inner surface 44 which defines a flow channel 42 communicating to the housing space 22 together with the first closing part 48, and the first tubular member 40 comprises at least a first marking part 46a and a second marking part 46b which are arranged at different positions from each other between the one end 40a of the first tubular member 40 and the first closing part 48 in an extending direction of the first tubular member 40.SELECTED DRAWING: Figure 1

Description

The present invention relates to a liquid containing container, a liquid containing container set, and a sterile joining kit.

Liquids such as cell suspensions, pharmaceutical-related liquids, and food-related liquids need to prevent contamination by foreign substances and bacteria. Therefore, when such a liquid is transferred from one storage container to another, the storage containers are connected to each other while maintaining the inside of these storage containers in a closed state with respect to the external environment. There is a need to do. In such a case, an aseptic joining device as described in Patent Document 1 can be used. In the aseptic joining device described in Patent Document 1, the first tube and the second tube are overlapped and fixed, and these tubes are fusing using a heating member, and then the fusing end of the first tube and the second tube are used. It is a device that replaces and joins the blown ends of tubes. By joining the tubes extending from the two storage containers using such an aseptic joining device, it becomes possible to transfer liquid between the storage containers via the liquid feeding pipe formed by the joined tubes.

Japanese Patent No. 697025

When moving a liquid from one storage container to the other storage container via a liquid delivery pipe, the liquid may remain inside the liquid supply pipe. The amount of liquid flowing into the other storage container also changes depending on the amount of liquid remaining in the liquid supply pipe. The amount of liquid remaining in the liquid feed pipe usually increases as the liquid feed pipe becomes longer. Therefore, in order to keep the amount of liquid flowing into the storage container constant, it is desirable to keep the length of the liquid supply pipe constant. In addition, the length of the liquid feeding pipe also affects the ease of performing work such as liquid feeding. Therefore, it is desired to keep the length of the liquid feed pipe constant from the viewpoint of workability.

However, when the liquid feed pipe is formed between the storage containers using the aseptic joining device as described in Patent Document 1, the aseptic joining device is installed at which position of the first tube and the second tube to be joined by the operator. The position where the first tube and the second tube are blown and joined varies depending on the method, and the length of the liquid feeding tube formed thereby also varies.

The present invention has been made in view of these points, and an object of the present invention is to provide a liquid storage container capable of keeping the length of a liquid feed pipe formed by an aseptic joining device constant.

The liquid storage container according to one aspect of the present invention has a container body that defines a liquid storage space, a first tubular member whose one end is connected to the container body, and a first tubular member that closes the first tubular member. The first tubular member has an inner surface that defines a flow path communicating with the accommodation space together with the first closed portion, and the first tubular member is said to have the same closed portion. At least a first mark portion and a second mark portion arranged at different positions from each other between the one end of the first tubular member and the first closed portion in the stretching direction of the first tubular member are provided. ..

In the liquid storage container of the present invention, the length of the liquid feed pipe formed by the aseptic joining device can be made constant.

FIG. 1 is a schematic cross-sectional view including a longitudinal axis of a first tubular member showing an example of a liquid container according to an embodiment. FIG. 2 is a schematic cross-sectional view including a longitudinal axis of the first tubular member showing an example of the liquid storage container according to the embodiment. FIG. 3 is a schematic view showing an example of a cross section of the first tubular member in line AA of FIG. FIG. 4 is a schematic view showing an example of a cross section of the first tubular member in line BB of FIG. FIG. 5 is a schematic view showing another example of the cross section of the first tubular member in line BB of FIG. FIG. 6 is a schematic cross-sectional view including a longitudinal axis of the first tubular member showing an example of the liquid storage container according to the embodiment. FIG. 7 is a schematic cross-sectional view including a longitudinal axis of the first tubular member showing an example of the liquid storage container according to the embodiment. FIG. 8 is a schematic cross-sectional view including a longitudinal axis of the first tubular member and the second tubular member showing an example of the liquid storage container set according to the embodiment. FIG. 9 is a schematic view showing an example of a cross section of a mark portion of a first tubular member and a mark portion of a second tubular member that are in contact with each other on the CC line of FIG. FIG. 10 is a schematic view showing an example of a cross section of a mark portion of a first tubular member and a mark portion of a second tubular member that are in contact with each other on the CC line of FIG. FIG. 11 is a schematic view showing an example of a cross section of a mark portion of a first tubular member and a mark portion of a second tubular member that are fitted to each other in the CC line of FIG. FIG. 12 is a schematic cross-sectional view including a longitudinal axis of the first tubular member and the second tubular member showing an example of the mark portion of the first tubular member and the mark portion of the second tubular member that are fitted to each other. is there. FIG. 13 is a schematic view showing an example of a cross section of a mark portion of the first tubular member and a mark portion of the second tubular member in the line EE of FIG. FIG. 14 is a schematic cross-sectional view including a longitudinal axis of the first tubular member and the second tubular member showing an example of the first tubular member and the second tubular member installed in the aseptic joining device. FIG. 15 is a schematic cross-sectional view including a longitudinal axis of the liquid feeding pipe showing an example of a liquid feeding pipe formed by joining a first tubular member and a second tubular member with an aseptic joining device.

(1) Liquid storage container First, the liquid storage container according to the embodiment will be described. As shown in FIG. 1, the liquid storage container 1 according to the embodiment includes a container body 20 that defines a liquid storage space 22, a first tubular member 40 having one end 40a connected to the container body 20, and a first tubular member 40. It includes a first closing portion 48 that closes the tubular member 40 of 1. The first tubular member 40 has an inner surface 44 that defines a flow path 42 communicating with the accommodation space 22 together with the first closure 48. The first tubular member 40 is arranged at different positions from one end 40a of the first tubular member 40 and the first closing portion 48 in the extending direction (that is, the longitudinal axis direction) of the first tubular member 40. It also includes two marking portions 46a and 46b (first marking portion 46a and second marking portion 46b).

The container body 20 may have any structure and may be formed of any material as long as it defines a storage space 22 for aseptically storing the liquid. For example, as the container body 20, a soft bag with a processed film, a molded container by blow molding, injection molding, or the like, a glass container, or the like can be used. The container body 20 is preferably made of a transparent material that allows the presence or absence of liquid in the storage space 22 to be confirmed. For example, it may be formed from a polyolefin resin such as polyethylene or polypropylene, polyvinyl chloride, an ethylene vinyl acetate copolymer resin, C-FLEX (registered trademark), polystyrene, glass or the like. The material of the container body 20 may be the same as that of the first tubular member 40, or may be a material that can be welded to the first tubular member 40. As a result, the container body 20 and the first tubular member 40 can be connected without using an adhesive, so that contamination due to the adhesive can be avoided.

The container body 20 may be, for example, a cell storage container, a culture solution storage container, a culture container, or a waste liquid container. The storage space 22 defined in the container body 20 communicates with the flow path 42 in the first tubular member 40 through the opening 24 formed in the container body 20. Further, the container body 20 may have an injection port (not shown) for injecting a liquid into the storage space 22. The inlet may be any opening that can be closed after injecting liquid into the containment space 22.

The first tubular member 40 has a tubular shape having an inner surface 44 and an outer surface 45. The first tubular member 40 has an opening 47 at one end 40a. The first tubular member 40 is connected to the container body 20 so that the opening 47 of the first tubular member 40 and the opening 24 of the container body 20 communicate with each other.

The first closing portion (closing portion) 48 is not particularly limited as long as it closes (closes) the first tubular member 40. As an example of the first closed portion 48, a welded portion formed by crushing and welding the first tubular member 40 in the radial direction, a stopper packed in the first tubular member 40 (for example, a rubber stopper), and the like. Examples thereof include a film adhered to the other end 40b of the first tubular member 40 by a top seal, a cap attached to the other end 40b of the first tubular member 40, and a wall surface integrally molded with the first tubular member 40.

As shown in FIG. 1, the first closing portion 48 may be provided at the other end 40b of the first tubular member 40. Alternatively, as shown in FIG. 2, it may be provided between one end 40a and the other end 40b of the first tubular member 40. When the first closing portion 48 is provided on the other end 40b of the first tubular member 40, dust and the like are less likely to collect on the other end 40b. When the first closing portion 48 is provided between one end 40a and the other end 40b of the first tubular member 40, the first closing portion 48 is located inside the first tubular member 40, so that the first closing portion 48 is first closed. The risk that the portion 48 is damaged and the first tubular member 40 is opened can be reduced.

Both the inner surface 44 of the first tubular member 40 and the first closing portion 48 define a flow path 42 communicating with the accommodation space 22. The accommodation space 22 and the flow path 42 form one closed space. In the present application, the "closed space" is a space closed to the external environment so as to prevent contamination by foreign substances and bacteria in the external environment, for example, a closed space closed to the external environment. Means.

The first tubular member 40 in FIG. 1 has a substantially columnar shape extending in one direction, but the first tubular member 40 is not limited to this, and defines a liquid flow path 42 and is an aseptic joining device. It may have any shape that can be joined. For example, the first tubular member 40 may be bent or may have a branch. Further, the first tubular member 40 may have flexibility. The first tubular member 40 may have a length of 10 cm or more in the stretching direction from the viewpoint of facilitating the liquid feeding operation. The first tubular member 40 can be manufactured by extrusion molding, injection molding, blow molding or the like.

FIG. 3 shows a cross section of the first tubular member 40 in line AA of FIG. 1, that is, other than the mark portions 46a and 46b of the first tubular member 40 perpendicular to the stretching direction of the first tubular member 40. An example of the cross section of the part is shown. In this example, both the inner surface 44 and the outer surface 45 of the first tubular member 40 have a circular cross-sectional shape. However, the cross-sectional shape of the inner surface 44 and the outer surface 45 of the first tubular member 40 is not limited to a circle, and may be any shape such as an ellipse or a polygon.

The first tubular member 40 is made of any material that can be joined by a sterile joining device described later. For example, the first tubular member 40 may be formed of a heat-weldable synthetic resin. Further, the first tubular member 40 may have such transparency that the interface between the liquid and the air in the flow path 42 can be visually recognized from the outside. Thereby, the presence or absence of the liquid in the flow path 42 can be determined. Specifically, as the material of the first tubular member 40, polyolefins such as polyvinyl chloride, C-FLEX (registered trademark), ethylene vinyl acetate copolymer resin, polyethylene and polypropylene are exemplified.

The first tubular member 40 has two marking portions 46a and 46b. The two marking portions 46a and 46b are separated from each other by a predetermined distance d between one end 40a of the first tubular member 40 and the first closing portion 48 in the extending direction of the first tubular member 40. Be placed. The predetermined distance d between the two marking portions 46a and 46b corresponds to the width D (see FIG. 14) of the holder 80 of the aseptic joining device described later.

The marks formed on the marks 46a and 46b have any visible form. In the example shown in FIG. 1, the mark is formed by the outer surface 45 of the first tubular member 40 being convex at the mark portions 46a and 46b. In the mark portions 46a and 46b, a groove (recess) serving as a mark may be formed on the outer surface 45 of the first tubular member 40. Printing such as direct printing or laser printing may be performed on the mark portions 46a and 46b. The mark portions 46a and 46b may be colored. In the mark portions 46a and 46b, a binding band or the like may be wound around the first tubular member 40 as a mark. The marks formed on the two mark portions may be separate or may be connected and integrated.

4 and 5 show the cross sections of the first tubular member 40 in the line BB of FIG. 1, that is, the mark portions 46a and 46b of the first tubular member 40 perpendicular to the stretching direction of the first tubular member 40. An example of the cross section of is shown.

In the example shown in FIG. 4, both the inner surface 44 and the outer surface 45 of the first tubular member 40 have a circular cross-sectional shape. The diameter of the outer surface 45 of the first tubular member 40 at the mark portions 46a and 46b is larger than the diameter of the outer surface 45 of the first tubular member 40 at the portions other than the mark portions 46a and 46b. As a result, the outer surface 45 of the mark portions 46a and 46b becomes convex to form a mark. The diameter of the inner surface 44 of the first tubular member 40 at the mark portions 46a and 46b is equal to the diameter of the inner surface 44 of the first tubular member 40 at the portions other than the mark portions 46a and 46b.

In the example shown in FIG. 5, the inner surface 44 of the first tubular member 40 has a circular cross-sectional shape, and the outer surface 45 has a triangular cross-sectional shape. The shape of the outer surface 45 of the first tubular member 40 at the mark portions 46a and 46b is different from the shape of the outer surface 45 of the first tubular member 40 at the portions other than the mark portions 46a and 46b. As a result, the outer surface 45 of the mark portions 46a and 46b becomes convex to form a mark. The diameter of the inner surface 44 of the first tubular member 40 at the mark portions 46a and 46b is equal to the diameter of the inner surface 44 of the first tubular member 40 at the portions other than the mark portions 46a and 46b.

In the examples shown in FIGS. 4 and 5, the outer shape of the cross section of the mark portions 46a and 46b of the first tubular member 40 perpendicular to the stretching direction of the first tubular member 40 is in the stretching direction of the first tubular member 40. It is different from the outer shape of the cross section of the portion other than the mark portions 46a and 46b of the vertical first tubular member 40. As a result, the outer surface 45 is convex at the mark portions 46a and 46b, and the mark is formed. Here, "the outer shape is different" means that the outer shape and / or the dimension is different. The outer shape of the cross section of the mark portions 46a and 46b of the first tubular member 40 perpendicular to the stretching direction of the first tubular member 40 is not limited to the circular shape and the triangular shape as shown in FIGS. , Square shape, polygonal shape and the like. Further, as will be described later, the mark portions 46a and 46b may have a holder fitting portion for fitting with the holder of the aseptic joining device, or the second tubular member to be joined to the first tubular member 40. It may have a shape that abuts or fits with the mark portion on the surface.

One of the mark portions 46a and 46b (first mark portion 46a) may have a different shape from the other (second mark portion 46b). For example, the first mark portion 46a has an outer shape different from that of the second mark portion 46b in a cross section perpendicular to the stretching direction of the first tubular member 40 and / or a cross section parallel to the stretching direction of the first tubular member. May have. Further, of the two marking portions 46a and 46b, the first marking portion 46a closer to one end 40a of the first tubular member 40 has a smaller outer shape than the second marking portion 46b closer to the first closing portion 48. May have. In this case, the liquid feed pipe obtained by joining the first tubular member 40 to the second tubular member as described later includes the first mark portion 46a having a small outer shape, but the second having a large outer shape. The mark portion 46b is not included. Therefore, the workability of the liquid feed pipe is improved. Further, the first mark portion 46a and the second mark portion 46b may be colored in different colors.

In the example shown in FIGS. 1 to 5, the shape and dimensions of the inner surface 44 in the cross section of the mark portions 46a and 46b of the first tubular member 40 perpendicular to the stretching direction of the first tubular member 40 are the first. It is the same as the shape and dimensions of the inner surface 44 in the cross section of the portion other than the mark portions 46a and 46b of the first tubular member 40 perpendicular to the stretching direction of the tubular member 40. As a result, the flow of liquid in the flow path 42 is stabilized, and the generation of pulsating flow can be avoided. Note that "same shape and size" means that there is no difference in shape and size that affects the flow of liquid in the flow path 42, and the shape and size are not necessarily completely the same. Good.

FIG. 6 shows another example of the liquid storage container 1 according to the embodiment. In the example shown in FIG. 6, the diameter of the flow path 42 between the first marking portion 46a and the first closing portion 48 and in the vicinity of the first marking portion 46a (that is, in the extending direction of the first tubular member 40). The diameter of the inner surface 44 in the vertical cross section) is smaller than the diameter of the flow path 42 in the other parts. When such a first tubular member 40 is joined to a second tubular member described later to form a liquid feed pipe, a flow path of a waste portion (a portion that does not constitute the liquid feed pipe) of the first tubular member 40. Since the volume of 42 is small, the amount of liquid discarded together with the discarded portion is small.

FIG. 7 shows yet another example of the liquid storage container 1 according to the embodiment. In the example shown in FIG. 7, the diameter of the flow path 42 between the first marking portion 46a and the first closing portion 48 and in the vicinity of the first marking portion 46a is larger than the diameter of the flow path 42 in the other portions. large. When such a first tubular member 40 is joined to a second tubular member described later to form a liquid feed pipe, the area of the joint surface between the first tubular member 40 and the second tubular member is large. It is possible to reliably join with high joining strength.

In addition to the two marking portions 46a and 46b, the first tubular member 40 may be provided with a further marking portion (not shown). That is, the first tubular member 40 includes at least two marking portions 46a and 46b. The additional markings may have the same shape and dimensions as the two markings 46a, 46b, or may have different shapes and dimensions.

(2) Liquid Containing Container Set Next, the liquid storage container set according to the embodiment will be described. As shown in FIG. 8, the liquid storage container set 2 according to the embodiment has a second closure that closes the liquid storage container 1 according to the above-described embodiment, the second tubular member 60, and the second tubular member 60. A member 3 including a portion 68a and a third closing portion 68b is provided. The second tubular member 60 has an inner surface 64 that defines a flow path 62 between the second closure 68a and the third closure 68b. The second tubular member 60 has two marking portions 66a and 66b arranged at different positions between the second closing portion 68a and the third closing portion 68b in the extending direction of the second tubular member 60. (Third mark portion 66a and fourth mark portion 66b) are provided. The distance between the two marking portions 66a and 66b of the second tubular member is the same as the distance d between the two marking portions 46a and 46b of the first tubular member 40 of the liquid storage container 1. It corresponds to the width D (see FIG. 14) of the holder 80 of the aseptic joining device described later.

Since the shape and material of the second tubular member 60 are the same as those of the first tubular member 40 described above, the description thereof will be omitted. The second tubular member 60 preferably has the same shape as the first tubular member 40 and is made of the same material as the first tubular member 40. Further, the diameters of the inner and outer surfaces of the second tubular member between the two marking portions 66a and 66b are the diameters of the first tubular member 40 between the two marking portions 46a and 46b of the liquid storage container 1. It may be the same as the diameter of the inner and outer surfaces of. As a result, the first tubular member 40 and the second tubular member 60 can be reliably joined.

The second closed portion 68a and the third closed portion 68b may be arbitrary members, respectively. For example, it may be a welding portion, a stopper, a film, a cap, or a wall surface that closes the second tubular member 60, similarly to the first closing portion 48 of the liquid storage container 1. Alternatively, the second closing portion 68a and / or the third closing portion 68b may be a container body similar to the container body 20 of the liquid storage container 1 connected to one end of the second tubular member 60. Alternatively, it may be a liquid feeding mechanism such as a perista pump, a syringe pump, a diaphragm pump, or a pressurizing pump connected to one end of the second tubular member 60. The inner surface 64 of the second tubular member 60, the second closing portion 68a, and the third closing portion 68b together define a liquid flow path 62. The flow path 62 forms one closed space.

The second closing portion 68a and the third closing portion 68b may be provided at both ends of the second tubular member 60, or may be provided between both ends of the second tubular member 60. One of the second closing portion 68a and the third closing portion 68b may be provided at the end of the second tubular member 60, and the other may be provided between both ends of the second tubular member 60. Dust and the like are less likely to collect at the ends where the second closing portion 68a and / or the third closing portion 68b are provided. The second closing portion 68a and / or the third closing portion 68b provided between the ends of the second tubular member 60 is located inside the second tubular member 60 and is therefore less likely to be damaged.

One of the two markings of the second tubular member 60 (third marking 66a) hits one of the two markings of the first tubular member 40 (second marking 46b) in a plane. The other of the two markings (fourth marking 66b) of the second tubular member 60 has a contactable shape, and the other of the two markings of the first tubular member 40 (first). It may have a shape that allows surface contact with the mark portion 46a). An example of the shape of such a mark portion is shown in FIG. FIG. 9 shows a cross section of the first tubular member 40 and the second tubular member 60 in the CC line of FIG. 8, that is, perpendicular to the stretching direction of the first tubular member 40 and the second tubular member 60. An example of the cross section of the first mark portion 46a of the first tubular member 40 and the fourth mark portion 66b of the second tubular member 60 is shown. In the example shown in FIG. 9, the first mark portion 46a and the fourth mark portion 66b have contact surfaces 41 and 61 that can come into contact with each other, respectively. Similarly, the second marking portion 46b of the first tubular member 40 and the third marking portion 66a of the second tubular member 60 also have contact surfaces capable of contacting each other.

In the example shown in FIG. 9, the first mark portion 46a and the fourth mark portion 66b that are in contact with each other have a circular cross section, but are not limited to this, and may have a cross section of any shape. .. For example, as shown in FIG. 10, the first mark portion 46a and the fourth mark portion 66b that are in contact with each other are a plane including the longitudinal axis of the first tubular member 40 and the longitudinal axis of the second tubular member 60. A plane parallel to may be formed. In this case, by bringing this surface into contact with a plate-shaped member such as a holder, the first mark portion 46a and the fourth mark portion 66b can be easily held while being in contact with each other. Similarly, the second mark portion 46b and the third mark portion 66a that are in contact with each other may have a cross section having an arbitrary shape.

Further, one of the two marking portions of the second tubular member 60 (third marking portion 66a) fits into one of the two marking portions of the first tubular member 40 (second marking portion 46b). The other of the two markings (fourth marking 66b) of the second tubular member 60 has a conformable shape, and the other of the two markings of the first tubular member 40 (first). It may have a shape that can be fitted to the mark portion 46a). Examples of the shape of such a mark portion are shown in FIGS. 11 to 13. FIG. 11 shows a cross section of the first tubular member 40 and the second tubular member 60 in the CC line of FIG. 8, that is, perpendicular to the stretching direction of the first tubular member 40 and the second tubular member 60. An example of the cross section of the first mark portion 46a of the first tubular member 40 and the fourth mark portion 66b of the second tubular member 60 is shown. FIG. 12 shows an example of the two marking portions 46a and 46b of the first tubular member 40 and the two marking portions 66a and 66b of the second tubular member 60, and the longitudinal axis of the first tubular member 40 and It is the schematic sectional drawing including the longitudinal axis of the 2nd tubular member 60. In FIG. 12, the first mark portion 46a and the fourth mark portion 66b are fitted to each other, and the second mark portion 46b and the third mark portion 66a are fitted to each other. FIG. 13 shows an example of the cross section of the first mark portion 46a and the fourth mark portion 66b on the line EE of FIG. In the example shown in FIGS. 11 to 13, the first mark portion 46a and the fourth mark portion 66b have uneven surfaces 43 and 63 that are fitted to each other, respectively. Similarly, the second mark portion 46b and the third mark portion 66a also have uneven surfaces that fit each other.

The first mark portion 46a and the fourth mark portion 66b, and the second mark portion 46b and the third mark portion 66a fitted to each other may have a cross section having an arbitrary shape. For example, as shown in FIG. 13, the first mark portion 46a and the fourth mark portion 66b fitted to each other are flat surfaces including the longitudinal axis of the first tubular member 40 and the longitudinal axis of the second tubular member 60. A plane parallel to may be formed. In this case, by bringing this surface into contact with a plate-shaped member such as a holder, the first mark portion 46a and the fourth mark portion 66b can be easily held while being fitted.

The first mark portion 46a and the third mark portion 66a may have the same shape, and the second mark portion 46b and the fourth mark portion 66b may have the same shape. In this case, the shape formed by the first mark portion 46a and the fourth mark portion 66b that are in contact with each other or fitted to each other, and the second mark portion 46b and the third mark portion 66a that are in contact with or fit to each other. Will have the same shape as the shape formed by. As a result, between the first mark portion 46a and the second mark portion 46b, that is, between the third mark portion 66a and the fourth mark portion 66b, the first tubular member 40 and the second tubular member Since the interval δ of 60 (see FIG. 12) is constant, the first tubular member 40 and the second tubular member 60 can be reliably joined. Further, the first mark portion 46a and the third mark portion 66a may have a smaller outer shape than the second mark portion 46b and the fourth mark portion 66b. In this case, the liquid feed pipe obtained by joining the first tubular member 40 and the second tubular member 60 as described later has a first mark portion 46a and a third mark portion 66a having a small outer shape. However, it does not have a second mark portion 46b and a fourth mark portion 66b having a large outer shape. Since such a liquid feed pipe has small irregularities, workability is good.

Further, a color (for example, the same color) and / or a corresponding character (for example, the same character) corresponding to the first mark portion 46a and the fourth mark portion 66b are attached, and the second mark portion 46b and the third mark portion 46b and the third mark portion 46b are attached. A color and / or character corresponding to the mark portion 66a may be added.

The first mark portion 46a and the fourth mark portion 66b, the second mark portion 46b and the third mark portion 66a may be formed into corresponding shapes (for example, a shape in which they are in contact with each other or a shape in which they are fitted to each other). By adding the corresponding color and / or the corresponding character, the worker who joins the first tubular member 40 and the second tubular member 60 can surely make sure that the first mark portion 46a and the fourth are designated. The mark portion 66b can be overlapped and the second mark portion 46b and the third mark portion 66a can be overlapped and joined (that is, the first mark portion 46a and the third mark portion 66a are mistakenly overlapped). In addition, it is possible to prevent the second mark portion 46b and the fourth mark portion 66b from being overlapped and joined), so that the first tubular member 40 and the second tubular member 60 are joined in the wrong direction. Can be prevented. In addition, it is possible to prevent the first tubular member 40 and / or the second tubular member 60 from being accidentally joined to another member.

The second tubular member 60 may be provided with a further marking portion in addition to the two marking portions. That is, the second tubular member 60 includes at least two marking portions. The additional markings may have the same shape, dimensions, color, etc. as the two markings, or may have different shapes, dimensions, colors, and the like.

(3) Aseptic Bonding Kit Next, the sterile joining kit according to the embodiment will be described. The aseptic joining kit according to the embodiment includes a liquid storage container set according to the above-described embodiment and a sterile joining device including a holder for holding a first tubular member and a second tubular member of the liquid storage container set. Have. In the aseptic joining device, the first tubular member and the second tubular member are provided so that the two markings of the first tubular member and the two markings of the second tubular member are both located at both ends of the holder. It can hold a tubular member. That is, the width of the holder of the aseptic joining device is the same as the distance between the two markings of the first tubular member and the distance between the two markings of the second tubular member.

As the aseptic joining device, any aseptic joining device can be used. For example, TSCD-II (manufactured by Terumo), SCDIIB (manufactured by Terumo), HOTNAVI (manufactured by JMS), BioWelder (manufactured by Sartorius) and the like can be used.

At least one of the mark portions of the first tubular member and at least one of the mark portions of the second tubular member may be provided with a holder fitting portion that can be fitted with the holder of the sterile joining device.

(4) Aseptic Joining Method An aseptic joining method using the liquid storage container, the liquid storage container set, and the sterile joining kit according to the embodiment will be described with reference to FIGS. 14 and 15.

As shown in FIG. 14, the first tubular member 40 and the second tubular member 60 are held by the holder 80 of the aseptic joining device. At this time, the first tubular member 40 is positioned with respect to the holder 80 so that the two marking portions 46a and 46b of the first tubular member 40 are located at both ends of the holder 80. Similarly, the second tubular member 60 is positioned with respect to the holder 80 so that the two marking portions 66a and 66b of the second tubular member 60 are located at both ends of the holder 80.

The aseptic joining device is then operated according to the method of use of the sterile joining device. The aseptic joining device cuts the first tubular member 40 and the second tubular member 60 while maintaining the flow path 42 of the first tubular member 40 and the flow path 62 of the second tubular member 60 in a closed space. Then, the cut portions of the first tubular member 40 and the second tubular member 60 are joined to each other. As a result, as shown in FIG. 15, the first tubular member 40 and the second tubular member 60 are joined via the joint portion 58, and the liquid feeding solution including the first tubular member 40 and the second tubular member 60 is formed. The tube 50 is formed. The liquid supply pipe 50 has a liquid supply passage 52 formed in which the flow path 42 of the first tubular member 40 and the flow path 62 of the second tubular member 60 communicate with each other. As a result, liquid can be fed via the liquid feeding path 52. The liquid may be fed by using a liquid feeding mechanism such as a perista pump, a syringe pump, a diaphragm pump, or a pressurizing pump, or by using gravity.

The first and second tubular members 40 and 60 are provided with mark portions 46a, 46b, 66a, 66b indicating the installation position of the holder 80 of the aseptic joining device. Therefore, the operator performing aseptic joining can visually and / or tactilely recognize which position of the first and second tubular members 40 and 60 should be held by the holder 80. Therefore, the length of the liquid feed pipe 50 formed is constant regardless of the operator.

As shown in FIG. 14, when the outer surfaces 45 and 65 of the first and second tubular members 40 and 60 are convex at the mark portions 46a, 46b, 66a and 66b, the holder 80 of the aseptic joining device is The holder 80 is sandwiched between the two marking portions 46a and 46b of the first tubular member 40 and the two marking portions 66a and 66b of the second tubular member 60, so that the holder 80 is sandwiched between the first tubular member 40 and the second tubular member 40. The holder 80 and the first and second tubular members 40 and 60 are prevented from being relatively displaced by sliding in the extending direction of the tubular member 60.

When the cross-sectional shape of the mark portions 46a, 46b, 66a, 66b perpendicular to the stretching direction of the first and second tubular members 40, 60 is anisotropic (that is, when the cross-sectional shape is other than a circle). For example, when the cross-sectional shape is triangular as in the example shown in FIG. 5, the orientations of the mark portions 46a, 46b, 66a, 66b (rotation angles of the mark portions 46a, 46b about the longitudinal axis of the first tubular member 40). , And the rotation angles of the mark portions 66a and 66b about the longitudinal axis of the second tubular member 60), so that the twists of the first and second tubular members 40 and 60 can be visually and tactilely recognized, respectively. .. Therefore, it is possible to prevent the first and second tubular members 40 and 60 from being joined in a twisted state.

In FIG. 14, the mark portions 46a, 46b, 66a, 66b have holder fitting portions 49a, 49b, 69a, 69b that can be fitted with the holder 80, respectively. Thereby, the position of the holder 80 of the aseptic joining device is more accurately determined at which position of the first tubular member 40 and the second tubular member 60. Further, the orientations of the mark portions 46a, 46b, 66a, 66b with respect to the holder 80 (the rotation angle of the mark portions 46a, 46b about the longitudinal axis of the first tubular member 40, and the longitudinal axis of the second tubular member 60. Since the rotation angles of the mark portions 66a and 66b at the center) are constant, it is possible to prevent the first tubular member 40 and the second tubular member 60 from being joined in a twisted state. The holder fitting portions 49a, 49b, 69a, 69b are not essential components. That is, a holder fitting portion may be provided on one of the two marking portions 46a and 46b of the first tubular member 40 and one of the two marking portions 66a and 66b of the second tubular member 60. However, it is not necessary to provide the holder fitting portion on any of the first and second tubular members 40 and 60.

In order to operate the aseptic joining device to form the liquid feed pipe 50, the first tubular member 40 and the second tubular member 60 are placed in the holder 80 in a predetermined orientation and position determined by the aseptic joining device. Need to be installed. For example, in the example shown in FIG. 14, on the paper of FIG. 14, the right side is the first tubular member 40 closed by the first closing portion 48, and the left side is the second closed by the second closing portion 68a. It needs to be positioned above the tubular member 60. If the aseptic joining device is operated with the first and second tubular members 40 and 60 turned upside down, the first tubular member 40 and the second tubular member 60 are cut, and then the first tubular member 40 and 60 are cut. The portion of the member 40 including the first closure 48 and the portion of the second tubular member 60 including the second closure 68a are joined to include the first closure 48 of the first tubular member 40. The missing portion and the portion of the second tubular member that does not include the second closing portion 68a are not joined to each other. As a result, the liquid feed pipe 50 cannot be obtained. Therefore, the first tubular member 40 and the second tubular member 60 need to be installed in the correct orientation and position.

Here, as in the example shown in FIGS. 9 to 13, the first mark portion 46a and the second mark portion 46b of the first tubular member 40 are the fourth marks of the second tubular member 60, respectively. When having a shape corresponding to the portion 66b and the third marking portion 66a (for example, a shape that can be contacted by a surface or a shape that can be fitted), the first marking portion 46a and the second marking portion 46a of the first tubular member 40 The mark portion 46b of the first tubular member 40 and the second tubular member 60 are brought into contact with or fitted to the fourth mark portion 66b and the third mark portion 66a of the second tubular member 60, respectively. The tubular member 60 is installed in the holder 80. Therefore, it is possible to prevent the first tubular member 40 and the second tubular member 60 from being installed in the holder 80 in a state different from the predetermined orientation and position. Further, since the rotation angle of the first tubular member 40 and the second tubular member 60 about the longitudinal axis can be made constant, the first and second tubular members 40 and 60 are joined in a twisted state. It can also be prevented from being done.

(5) Use of liquid storage container The liquid storage container according to the embodiment is usually used for storing a liquid that requires aseptic handling. For example, a culture container for culturing cells, a medium supply container for supplying a medium, a cell recovery container for collecting a cell suspension after culturing, a waste liquid container for collecting a medium during or after culturing, or a temporary cell. The liquid storage container according to the embodiment can be used as a whole container related to cells such as a storage container or in a cell culture system. In particular, it can be suitably used for a cell culture system. Other liquids that require aseptic handling include, for example, food-related liquids, pharmaceutical-related liquids, and the like. Examples of food-related liquids include beverages, seasonings and the like. Pharmaceutical-related liquids include electrolyte infusions such as physiological saline, sugar injections such as glucose, blood preparations, antibiotics, proteinaceous drugs such as antibodies, low molecular weight proteins, peptide drugs such as hormones, and nucleic acid drugs. Cellular drugs, vaccines to prevent various infectious diseases, steroids, insulin, anticancer agents, proteolytic enzyme inhibitors, analgesics, antipyretic analgesics and anti-inflammatory agents, anesthetics, fat emulsions, antihypertensive agents, vasodilators, heparin Examples thereof include injection solutions for correcting electrolytes such as sodium chloride and potassium lactate, vitamins, and contrasting agents. However, the use of the liquid storage container is not limited to these.

Hereinafter, the present invention will be specifically described with reference to Examples, but the present invention is not limited to these Examples.

As the first and second tubular members, two tubes having an inner diameter of 3 mm and an outer diameter of 4 mm were prepared. Two markings were formed by wrapping and fixing two binding bands around each tube. In each tube, the distance between the markings was made equal to the width of the holder of the sterile joining device. Each tube was placed in the holder of the sterile joining device so that the two cable ties were located at both ends of the holder of the sterile joining device. Since the position of each tube with respect to the holder was fixed by the binding band, the tube did not move with respect to the holder even if the tube was pulled in the extending direction of the tube. When the aseptic joining device was operated, the two tubes were aseptically joined.

Although the embodiments of the present invention have been described in detail above, the present invention is not limited to the above embodiments, and various design changes are made without departing from the spirit of the present invention described in the claims. It can be performed.

1 Liquid storage container 20 Container body 22 Storage space 24 Opening 40 First tubular member 41, 61 Contact surface 42, 62 Flow path 43, 63 Concavo-convex surface 44 Inner surface 45, 65 Outer surface 46a, 46b, 66a, 66b Marks Part 47 Opening 48 First closing part 49a, 49b, 69a, 69b Holder fitting part 50 Liquid feeding pipe 52 Liquid feeding path 58 Joint part 60 Second tubular member 68a Second closing part 68b Third closing part 80 holder

Claims (9)

It is a liquid storage container
The container body that defines the liquid storage space,
A first tubular member whose one end is connected to the container body,
A first closing portion that closes the first tubular member,
With
The first tubular member has an inner surface that defines a flow path communicating with the accommodation space together with the first closure.
A first marker portion in which the first tubular member is arranged at different positions from each other between the one end of the first tubular member and the first closed portion in the extending direction of the first tubular member. A liquid container having at least a second marker and a second marker. The outer shape of the cross section of the first mark portion and the second mark portion of the first tubular member, which is perpendicular to the stretching direction of the first tubular member, is perpendicular to the stretching direction of the first tubular member. The liquid container according to claim 1, which is different from the outer shape of the cross section of the portion of the first tubular member other than the first mark portion and the second mark portion. The shape and dimensions of the inner surface of the first tubular member in the cross section of the first mark and the second mark of the first tubular member, which is perpendicular to the stretching direction of the first tubular member. Is the inside of the first tubular member in a cross section of a portion of the first tubular member other than the first mark portion and the second mark portion, which is perpendicular to the stretching direction of the first tubular member. The liquid containing container according to claim 1, which has the same surface shape and dimensions. The liquid storage container according to any one of claims 1 to 3.
A second tubular member and a member having a second closing portion and a third closing portion for closing the second tubular member.
It is a liquid storage container set equipped with
The second tubular member has an inner surface that defines a flow path between the second closure and the third closure.
A third marker and a fourth in which the second tubular member is arranged at different positions between the second closure and the third closure in the extending direction of the second tubular member. With at least the mark of
The distance between the third mark portion and the fourth mark portion is the same as the distance between the first mark portion and the second mark portion of the first tubular member of the liquid storage container. Storage container set. The first mark portion has the same shape as the third mark portion, and has the same shape as the third mark portion.
The liquid storage container set according to claim 4, wherein the second mark portion has the same shape as the fourth mark portion. The first mark portion can come into contact with the fourth mark portion on a surface.
The liquid storage container set according to claim 4 or 5, wherein the second mark portion can come into contact with the third mark portion on a surface. The first mark portion can be fitted with the fourth mark portion, and the first mark portion can be fitted with the fourth mark portion.
The liquid storage container set according to claim 4 or 5, wherein the second mark portion can be fitted with the third mark portion. The liquid storage container set according to any one of claims 4 to 7.
An aseptic joining kit comprising a aseptic joining device including a first tubular member and a holder for holding a second tubular member of the liquid storage container set.
In the aseptic joining device, the first mark portion and the second mark portion of the first tubular member and the third mark portion and the fourth mark portion of the second tubular member are both both ends of the holder. A sterile joining kit capable of holding the first tubular member and the second tubular member so as to be located in. At least one of the first mark portion and the second mark portion of the first tubular member and at least one of the third mark portion and the fourth mark portion of the second tubular member are with the holder. The aseptic bonding kit according to claim 8, further comprising a matable holder fitting portion.

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