JP2020150819A - Storage container - Google Patents
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Abstract
Description
本発明は、細胞培養のための培養容器を収納する収納容器に関する。 The present invention relates to a storage container for storing a culture container for cell culture.
再生医療や遺伝子治療、創薬等の分野で、細胞の大量培養が求められている。ｉＰＳ細胞やＥＳ細胞を使用した再生医療において、細胞移植による治療を行うためには、一人あたり１０の９乗個以上の分化細胞が必要になると考えられている。こういった大量の細胞を培養するために、従来ではフラスコ等の容器に貯留された培地の中で大量の細胞を培養する浮遊培養法が用いられてきた。しかしながら、浮遊培養法では、継代時の細胞生存率の低さや接着性細胞の自発的細胞塊の融合等により、治療に利用出来る細胞の割合が低くなってしまうという問題があった。 Mass culture of cells is required in the fields of regenerative medicine, gene therapy, drug discovery, and the like. In regenerative medicine using iPS cells and ES cells, it is considered that 10 to the 9th power or more differentiated cells are required per person in order to perform treatment by cell transplantation. In order to culture such a large amount of cells, a suspension culture method in which a large amount of cells are cultured in a medium stored in a container such as a flask has been conventionally used. However, the suspension culture method has a problem that the proportion of cells that can be used for treatment decreases due to the low cell viability at the time of passage and the fusion of spontaneous cell clusters of adhesive cells.
そこで、近年、従来の浮遊培養法に代わって、マルチウェルプレート等の培養容器を使用して、細胞を生体内に存在する状態に近い凝集塊の態様にして培養する三次元培養と呼ばれる培養方法が行われるようになってきた。この三次元培養法によって細胞を培養することで、培養細胞の歩留まりは向上したが、治療に必要な数の細胞を準備するには、大量の培養容器と、この培養容器を収容して所定の培養環境に維持する多数のＣＯ２インキュベータが必要である。さらに、ｉＰＳ細胞の場合、高清浄なＣＯ２インキュベータが必要となり、結果として細胞培養コストの増大につながる。 Therefore, in recent years, instead of the conventional suspension culture method, a culture method called three-dimensional culture is used in which cells are cultured in the form of agglomerates that are close to the state existing in the living body by using a culture container such as a multi-well plate. Has come to be done. By culturing cells by this three-dimensional culture method, the yield of cultured cells was improved, but in order to prepare the required number of cells for treatment, a large number of culture containers and a predetermined number of culture containers were accommodated. A large number of CO2 incubators are required to maintain the culture environment. Furthermore, in the case of iPS cells, a highly clean CO2 incubator is required, resulting in an increase in cell culture cost.
本発明は上記問題点に鑑みてなされたものであり、マルチウェルプレート等の一般的な培養容器であっても、内部を清浄な状態に維持することが可能であり、且つ、培養容器を高密度に収納することが出来る収納容器を提供することにある。 The present invention has been made in view of the above problems, and even a general culture container such as a multi-well plate can maintain the inside in a clean state, and the culture container is high. The purpose is to provide a storage container that can be stored at a high density.
上記課題を解決するために、本発明の収納容器は、培養容器が通過可能な開口が形成された本体と、前記開口を気密に閉鎖可能な蓋体とで構成される収納容器であって、前記本体は、前記本体に形成された通気口をガス流通可能に閉鎖するフィルタ部材と、前記本体の内部空間において、鉛直方向に所定の間隔を空けて配置される複数の棚板と、を備え、前記複数の棚板は、前記培養容器の側面壁に突出して形成される一対の鍔部とそれぞれ係合することで前記培養容器を鉛直方向に支持することを特徴としている。 In order to solve the above problems, the storage container of the present invention is a storage container composed of a main body having an opening through which the culture container can pass and a lid through which the opening can be closed airtightly. The main body includes a filter member that closes a vent formed in the main body so that gas can flow, and a plurality of shelves arranged at predetermined intervals in the vertical direction in the internal space of the main body. The plurality of shelves are characterized in that the culture container is supported in the vertical direction by engaging with a pair of flanges formed so as to project from the side wall of the culture container.
上記構成とすることで、限られた収納空間の中に培養容器を多数収納することが出来、さらに、フィルタ部材が外部環境に浮遊している塵埃や殺菌などの収納容器内部への侵入を防止するので、収納容器の内部空間は清浄な状態に維持される。 With the above configuration, a large number of culture containers can be stored in the limited storage space, and the filter member prevents dust and sterilization floating in the external environment from entering the storage container. Therefore, the internal space of the storage container is maintained in a clean state.
また、本発明の収納容器に形成される棚板は、培養容器が前記本体内に収納状態にあるとき、支持対象の前記培養容器の底部よりも上方であり、且つ、前記培養容器の頂部よりも下方に位置することを特徴としている。上記構成とすることで、棚板は収納された培養容器の底部と頂部との間に位置することになるので、収容された複数の培養容器の上下方向の間隔を棚板の厚み寸法よりも小さくすることが出来る。 Further, the shelf plate formed in the storage container of the present invention is above the bottom of the culture container to be supported and from the top of the culture container when the culture container is stored in the main body. Is also characterized by being located below. With the above configuration, the shelf board is located between the bottom and the top of the stored culture container, so that the vertical distance between the stored culture containers is larger than the thickness dimension of the shelf board. It can be made smaller.
また、本発明の収納容器を構成本体の奥行寸法は、前記培養容器の奥行寸法よりも小さいことを特徴としている。上記構成とすることで、作業者は、培養容器の本体から突出した部分を保持することで、培養容器を収納容器の本体に対して容易に出し入れすることが出来る。 Further, the depth dimension of the main body of the storage container of the present invention is smaller than the depth dimension of the culture container. With the above configuration, the operator can easily put the culture container in and out of the main body of the storage container by holding the portion protruding from the main body of the culture container.
また、本発明の収納容器は、所定の高さ寸法を有する第１の培養容器と、前記第１の培養容器の前記高さ寸法の約１／２の高さ寸法を有する第２の培養容器のいずれも収納可能であることを特徴としている。なお、第１の培養容器の高さ寸法を約１２．８ｍｍとした場合、高さ寸法が約６．４ｍｍに設定された第２の培養容器を、第１の培養容器の２倍の数収納することが出来る。この時、第１の収納容器と第２の収納容器のいずれも、各棚板によって支持される。 In addition, the storage container of the present invention includes a first culture container having a predetermined height dimension and a second culture container having a height dimension of about 1/2 of the height dimension of the first culture container. The feature is that all of them can be stored. When the height dimension of the first culture vessel is about 12.8 mm, the number of the second culture vessel whose height dimension is set to about 6.4 mm is twice as many as that of the first culture vessel. Can be done. At this time, both the first storage container and the second storage container are supported by the respective shelf boards.
また、本発明の収納容器の本体は、ポリエチレン製であることを特徴としている。さらに、本発明の収納容器の蓋体は、ポリエチレン製であることを特徴としている。上記構成とすることで、蓋体の本体への着脱が容易になり、また、収納された培養容器の収納容器外部からの観察が容易になる。さらに、過酸化水素ガス等の滅菌ガスでの滅菌処理が可能になる。 Further, the main body of the storage container of the present invention is characterized in that it is made of polyethylene. Further, the lid of the storage container of the present invention is characterized in that it is made of polyethylene. With the above configuration, the lid can be easily attached to and detached from the main body, and the stored culture container can be easily observed from the outside of the storage container. Further, sterilization treatment with a sterilizing gas such as hydrogen peroxide gas becomes possible.
本発明によれば、収納容器は、培養容器を内部に高密度に収納することが出来るので、ＣＯ２インキュベータ等の培養装置の限られた培養空間に多数の培養容器を投入することが出来る。また、収納容器に備えられるガス流通可能なフィルタ部材により、培養空間内の水蒸気やＣＯ２ガスは収納容器内部まで到達し、さらに、塵埃や雑菌等は収納容器内部への侵入を阻止される。これにより、比較的清浄度の低いＣＯ２インキュベータであっても、細胞等の安全かつ大量の培養が可能になった。 According to the present invention, since the storage container can store the culture container at a high density inside, a large number of culture containers can be put into the limited culture space of the culture device such as a CO2 incubator. Further, the gas flowable filter member provided in the storage container allows water vapor and CO2 gas in the culture space to reach the inside of the storage container, and further, dust and germs are prevented from entering the inside of the storage container. This has made it possible to safely and mass-culture cells and the like even in a CO2 incubator with relatively low cleanliness.
以下に、本発明の実施の形態を、図を参照しながら詳しく説明する。図１は本発明の一実施形態である収納容器１を示す斜視図であり、図２は収納容器１に収納される第１の培養容器２を示す図である。第１の培養容器２は、上面視略矩形に成型されたマイクロプレート型シャーレであり、四方の壁面に分画された領域に細胞や培地等の試料を収容するものである。本実施形態の第１の培養容器２は、生体分子スクリーニング協会（ＳＢＳ：Ｓｏｃｉｅｔｙ ｆｏｒ Ｂｉｏｍｏｌｅｃｕｌａｒ Ｓｃｒｅｅｎｉｎｇ）によって開発され、米国国家規格協会（ＡＮＳＩ：Ａｍｅｒｉｃａｎ Ｎａｔｉｏｎａｌ Ｓｔａｎｄａｒｄｓ Ｉｎｓｔｉｔｕｔｅ）によって規定された基準に則って製作されたものであり、横方向の寸法Ｗと奥行方向の寸法Ｄがそれぞれ８５．５ｍｍ、１２７．８ｍｍであり、高さ方向の寸法Ｈが１２．８ｍｍで製作されている。また、培養領域の四方を囲う壁のうち長辺となる２つの壁１８の上端部と下端部には、壁１８から突出するように上鍔部１６と下鍔部１７が形成されている。培養容器２の上端部に形成される上鍔部１６は、厚み寸法が１ｍｍであり、側面壁１８から４ｍｍ突出するように形成されている。また、培養容器２の下端部に形成される下鍔部１７は、厚み寸法が３ｍｍであり、側面壁１８から４ｍｍ突出するように形成されている。なお、第１の培養容器２は、マイクロプレート型シャーレの他に、容器表面にウェルと呼ばれる複数の窪みが形成されたものであってもよい。ウェルの数は９６、３８４、１５３６等多数の種類があるが、本発明は、外形が培養容器２と同一であればあらゆるタイプの容器に対応可能である。なお培養容器２は、自動搬送装置や検査装置等の利便性を考慮すると、ＡＮＳＩ／ＳＢＳ規格に準拠したものであることが望ましい。さらに、本実施形態の培養容器２は、ポリスチレンに代表される透明度に優れた樹脂で製作されていて、培養途中の細胞の顕微鏡での観察やプレートリーダーでの測定に適している。 Hereinafter, embodiments of the present invention will be described in detail with reference to the drawings. FIG. 1 is a perspective view showing a storage container 1 according to an embodiment of the present invention, and FIG. 2 is a view showing a first culture container 2 stored in the storage container 1. The first culture vessel 2 is a microplate-type petri dish molded into a substantially rectangular shape when viewed from above, and stores samples such as cells and a medium in regions partitioned on four wall surfaces. The first culture vessel 2 of the present embodiment was developed by the Biomolecule Screening Association (SBS: Society for Biomolecular Screening) and manufactured according to the standards specified by the American National Standards Institute (ANSI). The dimensions W in the lateral direction and the dimension D in the depth direction are 85.5 mm and 127.8 mm, respectively, and the dimension H in the height direction is 12.8 mm. Further, an upper collar portion 16 and a lower collar portion 17 are formed on the upper end portion and the lower end portion of the two walls 18 which are the long sides of the walls surrounding the four sides of the culture region so as to protrude from the wall 18. The upper collar portion 16 formed at the upper end portion of the culture vessel 2 has a thickness dimension of 1 mm and is formed so as to project 4 mm from the side wall 18. Further, the lower collar portion 17 formed at the lower end portion of the culture vessel 2 has a thickness dimension of 3 mm and is formed so as to project 4 mm from the side wall 18. In addition to the microplate type petri dish, the first culture vessel 2 may have a plurality of depressions called wells formed on the surface of the vessel. There are many types of wells such as 96, 384, and 1536, but the present invention can be applied to any type of container as long as the outer shape is the same as that of the culture container 2. It is desirable that the culture vessel 2 conforms to the ANSI / SBS standard in consideration of the convenience of the automatic transfer device, the inspection device, and the like. Further, the culture vessel 2 of the present embodiment is made of a resin having excellent transparency typified by polystyrene, and is suitable for observation of cells during culture with a microscope and measurement with a plate reader.
図３は収納容器１に収納される第２の培養容器３を示す図である。第２の培養容器３は、第１の培養容器３と同じ横方向の寸法Ｗと奥行方向の寸法Ｄを有する板状の培養器具である。また、第１の培養容器１と同様に、上面視略矩形に成型されたマイクロプレート型シャーレであり、四方の壁面に分画された領域に細胞や培地等の試料を収容するものである。また、第２の培養容器３の高さ方向の寸法Ｈ´は６．１ｍｍであり、第１の培養容器２の約１／２の高さ寸法を有している。また、第２の培養容器３の培養領域の四方を囲う壁のうち長辺となる２つの壁１９の上端部と下端部には、壁面から突出するように上鍔部２０と下鍔部２１が形成されている。培養容器３の上端に形成される上鍔部２０は、厚み寸法が１ｍｍであり、側面壁１９から４ｍｍ突出するように形成されている。培養容器３の下端に形成される下鍔部２１は、厚み寸法が２ｍｍであり、側面壁１９から４ｍｍ突出するように形成されている。なお、第２の培養容器３には、マイクロプレート型シャーレの他に、容器表面にウェルと呼ばれる複数の窪みが形成されたものであってもよい。ウェルの数は９６、３８４、１５３６等多数の種類があるが、本発明は、外形が培養容器３と同一であればあらゆるタイプの容器に対応可能である。なお、培養容器３はＡＮＳＩ／ＳＢＳ規格に準拠したものであることが望ましい。また、本実施形態の培養容器３は、ポリスチレンに代表される透明度に優れた樹脂で製作されていて、培養途中の細胞の顕微鏡での観察やプレートリーダーでの測定に適している。 FIG. 3 is a diagram showing a second culture container 3 stored in the storage container 1. The second culture vessel 3 is a plate-shaped culture instrument having the same lateral dimension W and depth dimension D as the first culture vessel 3. Further, like the first culture vessel 1, it is a microplate type petri dish molded into a substantially rectangular shape in the top view, and stores samples such as cells and a medium in the regions partitioned on the four wall surfaces. Further, the height dimension H'of the second culture vessel 3 in the height direction is 6.1 mm, which is about 1/2 the height dimension of the first culture vessel 2. Further, the upper end portion and the lower end portion 21 of the two walls 19 which are the long sides of the wall surrounding the four sides of the culture area of the second culture container 3 are provided with an upper collar portion 20 and a lower collar portion 21 so as to project from the wall surface. Is formed. The upper collar portion 20 formed at the upper end of the culture vessel 3 has a thickness dimension of 1 mm and is formed so as to project 4 mm from the side wall 19. The lower collar portion 21 formed at the lower end of the culture vessel 3 has a thickness dimension of 2 mm and is formed so as to project 4 mm from the side wall 19. In addition to the microplate type petri dish, the second culture vessel 3 may have a plurality of depressions called wells formed on the surface of the vessel. There are many types of wells such as 96, 384, and 1536, but the present invention can be applied to any type of container as long as the outer shape is the same as that of the culture container 3. It is desirable that the culture vessel 3 conforms to the ANSI / SBS standard. Further, the culture vessel 3 of the present embodiment is made of a resin having excellent transparency typified by polystyrene, and is suitable for observation of cells during culture with a microscope and measurement with a plate reader.
次に、本発明の一実施形態である収納容器１について説明する。図４は本実施形態の収納容器１を側面から見た断面図であり、図５は収容容器１を開口４が形成された正面から見た断面図である。本実施形態の収納容器１は、前面に開口４を有する略直方体の箱状に形成される本体５と、この開口４を気密に閉鎖可能な蓋体６とで構成される。本実施形態の本体５と蓋体６は、本体５に形成されたプラグ部１０の先端を蓋体６に形成されたソケット部２８に嵌合させる所謂インロー構造となっている。本体５の左右両側面、上部と底部、及び後面は壁で覆われていて、さらに、本体５の左右側壁には、所定の寸法の通気口７が設けられている。また、この通気口７を覆うようにフィルタ部材８が配置されている。本体５に形成される開口４は、培養容器２、３が水平状態で挿通可能な面積を有していて、さらに、開口４を規定する壁の周囲は、本体５の外形に対して断面積が小さくなるように段差９が設けられていて、この開口４の周囲の断面積が小さくなった部分１０を蓋体６に挿入することで、本体５の開口４は閉じられる。さらに、開口４を規定する壁１０の端部には、弾性部材からなるシール部材１１が開口４の周縁全体にわたって本体５に密着固定されていて、本体５と蓋体６は、このシール部材１１によって気密に閉鎖される。 Next, the storage container 1 which is an embodiment of the present invention will be described. FIG. 4 is a cross-sectional view of the storage container 1 of the present embodiment as viewed from the side surface, and FIG. 5 is a cross-sectional view of the storage container 1 as viewed from the front with the opening 4 formed. The storage container 1 of the present embodiment is composed of a main body 5 formed in a substantially rectangular parallelepiped box shape having an opening 4 on the front surface, and a lid 6 capable of airtightly closing the opening 4. The main body 5 and the lid 6 of the present embodiment have a so-called inlay structure in which the tip of the plug portion 10 formed in the main body 5 is fitted into the socket portion 28 formed in the lid 6. The left and right side surfaces, the top and bottom, and the rear surface of the main body 5 are covered with walls, and the left and right side walls of the main body 5 are provided with vents 7 having predetermined dimensions. Further, the filter member 8 is arranged so as to cover the vent 7. The opening 4 formed in the main body 5 has an area through which the culture vessels 2 and 3 can be inserted in a horizontal state, and the circumference of the wall defining the opening 4 has a cross-sectional area with respect to the outer shape of the main body 5. The opening 4 of the main body 5 is closed by inserting the portion 10 having a small cross-sectional area around the opening 4 into the lid 6 so that the step 9 is provided so as to reduce the size. Further, at the end of the wall 10 that defines the opening 4, a sealing member 11 made of an elastic member is closely fixed to the main body 5 over the entire peripheral edge of the opening 4, and the main body 5 and the lid 6 are the sealing member 11. Closed airtightly.
本実施形態の蓋体６は、一面に開口１２を有する略直方体の箱状に形成されていて、収容容器１の開口４を気密に閉鎖する。蓋体６の内側壁面には、蓋体６の奥側の断面積に対して開口１２側の断面積が大きくなるように段差１３が設けられていて、この開口１２周縁の、断面積が比較的大きくなった部分（ソケット部２８）に本体５のプラグ部１０が挿入されることで本体の開口４は閉じられる。また、この段差１３は本体５の開口４周縁のシール部材１１と対向する位置にあり、この段差１３と本体５が備えるシール部材１１とが当接することで、本体の開口４は気密に閉鎖される。さらに、本体５への蓋体６の装着を容易にするために、蓋体６の内周部１４は開口１２側に移動するにしたがって断面積が大きくなるテーパー状に形成されている。 The lid 6 of the present embodiment is formed in the shape of a substantially rectangular parallelepiped box having an opening 12 on one surface, and airtightly closes the opening 4 of the storage container 1. The inner wall surface of the lid 6 is provided with a step 13 so that the cross section on the opening 12 side is larger than the cross section on the back side of the lid 6, and the cross sections on the periphery of the opening 12 are compared. The opening 4 of the main body is closed by inserting the plug portion 10 of the main body 5 into the enlarged portion (socket portion 28). Further, the step 13 is located at a position facing the seal member 11 on the periphery of the opening 4 of the main body 5, and when the step 13 and the seal member 11 included in the main body 5 come into contact with each other, the opening 4 of the main body is airtightly closed. To. Further, in order to facilitate the attachment of the lid body 6 to the main body 5, the inner peripheral portion 14 of the lid body 6 is formed in a tapered shape in which the cross-sectional area increases as it moves toward the opening 12.
本体５と蓋体６とがシール部材１１を介して気密に閉鎖される位置に達すると、本体５のプラグ部１０に形成された半球状の突起２９と蓋体６のソケット部２８に形成された半円状の凹部３０とが、蓋体６の弾性力によって係合して、蓋体６と本体５とは固定される。図６を参照。本体５から蓋体６を取り外すには、蓋体６を本体５から離れる方向に引っ張ることで、蓋体６の凹部３０が突起２９を乗り越え、固定が解除される。なお、本発明はこの固定方法に限定されることはなく、多様な固定方法が適用可能である。 When the main body 5 and the lid 6 reach a position where they are airtightly closed via the seal member 11, hemispherical protrusions 29 formed on the plug portion 10 of the main body 5 and the socket portion 28 of the lid 6 are formed. The semi-circular recess 30 is engaged by the elastic force of the lid 6, and the lid 6 and the main body 5 are fixed. See FIG. To remove the lid 6 from the main body 5, the lid 6 is pulled away from the main body 5, so that the recess 30 of the lid 6 gets over the protrusion 29 and is released from fixing. The present invention is not limited to this fixing method, and various fixing methods can be applied.
本実施形態の収納容器１の本体５は、容器内部の細胞観察を可能にするために、高い透明度を有し、比較的強度が高く、さらに、過酸化水素ガス等の滅菌ガスにも耐性のあるポリスチレンで形成されている。また、本実施形態の蓋体６は、比較的高い柔軟性を有し、過酸化水素ガス等の滅菌ガスにも耐性のあるポリエチレンで形成されている。また、開口４を気密に閉鎖するシール部材１１は、柔軟性が高く過酸化水素ガス等の滅菌ガスにも耐性のあるシリコンゴムで形成されている。なお、本発明はこれらの材質に限定されることはなく、使用される状況に応じて適宜変更することが可能であることは言うまでもない。 The main body 5 of the storage container 1 of the present embodiment has high transparency, relatively high strength, and is resistant to sterilizing gas such as hydrogen peroxide gas in order to enable observation of cells inside the container. It is made of certain polystyrene. Further, the lid 6 of the present embodiment is made of polyethylene which has relatively high flexibility and is resistant to sterilizing gas such as hydrogen peroxide gas. Further, the sealing member 11 that airtightly closes the opening 4 is made of silicon rubber that is highly flexible and resistant to sterilizing gas such as hydrogen peroxide gas. Needless to say, the present invention is not limited to these materials, and can be appropriately changed depending on the situation in which the material is used.
本実施形態の収納容器１が備えるフィルタ部材８は、空気中を浮遊する雑菌や塵埃を透過させることなく、かつ、水蒸気やＣＯ２ガスを透過させる濾過精度を有することが必要である。さらに、過酸化水素ガス等の滅菌ガスに対する耐性を備えることが望ましい。なお、本実施形態の収納容器１が備えるフィルタ部材８は、ポリエチレン製の連続繊維で形成された不織布が使用されている。上記濾過精度を有するフィルタ部材８を備えることで、収納容器１の内部は、酸素濃度、ＣＯ２濃度、湿度等、所定の環境に維持され、且つ、塵埃や雑菌の侵入しない清浄な環境に維持される。 The filter member 8 included in the storage container 1 of the present embodiment is required to have filtration accuracy that allows water vapor and CO2 gas to permeate without allowing germs and dust floating in the air to permeate. Further, it is desirable to have resistance to sterilizing gas such as hydrogen peroxide gas. The filter member 8 included in the storage container 1 of the present embodiment is made of a non-woven fabric made of continuous polyethylene fibers. By providing the filter member 8 having the above filtration accuracy, the inside of the storage container 1 is maintained in a predetermined environment such as oxygen concentration, CO2 concentration, humidity, etc., and is maintained in a clean environment in which dust and germs do not invade. To.
本実施形態の収納容器１の内部空間は、培養容器２、３を、培養空間を上にした水平状態の姿勢で収納することが可能な幅寸法を有している。また、収納容器１の内部空間は、培養容器２を４個、第２の培養容器３を８個上下方向に収納することが可能な高さ寸法を有している。なお、収納容器１の本体５の奥行寸法は培養容器２、３の奥行寸法Ｄよりも短くなるように形成されていて、作業者は、培養容器２、３の本体５に収納されていない部分を保持することで本体５から培養容器２、３を容易に取り出すことが出来る。 The internal space of the storage container 1 of the present embodiment has a width dimension capable of storing the culture containers 2 and 3 in a horizontal posture with the culture space facing up. Further, the internal space of the storage container 1 has a height dimension capable of storing four culture containers 2 and eight second culture containers 3 in the vertical direction. The depth dimension of the main body 5 of the storage container 1 is formed to be shorter than the depth dimension D of the culture containers 2 and 3, and the worker is not stored in the main body 5 of the culture containers 2 and 3. The culture vessels 2 and 3 can be easily taken out from the main body 5 by holding the above.
本実施形態の本体５の内部には、培養容器１を鉛直方向に支持する棚板１５が左右対称の位置に配置されている。本実施形態の収納容器１は、前述した第１の培養容器２および第２の培養容器３を所定の数収納する容量を有している。本実施形態の収容容器１の内部空間に形成される左右一対の棚板１５は、厚さ寸法約２ｍｍの水平の板状に形成されていて、図面視して左右対称となるように、上下方向に６．８ｍｍのピッチで八段配置されている。図５を参照。また、最下段の棚板１５ａの下面３１と収納容器１の底部上面３２との間隔は約４ｍｍに設定されている。図７を参照。これにより、第１の培養容器２ａおよび、第２の培養容器３ａは、それぞれが備える下鍔部１７、２１が収納容器１の底部上面３２と最下段の棚板１５ａの下面３１との間に形成される空間に挿入されて、収納容器１の最も下に位置する収納位置に収納される。 Inside the main body 5 of the present embodiment, shelf boards 15 that support the culture vessel 1 in the vertical direction are arranged at symmetrical positions. The storage container 1 of the present embodiment has a capacity for storing a predetermined number of the above-mentioned first culture container 2 and the second culture container 3. The pair of left and right shelf boards 15 formed in the internal space of the storage container 1 of the present embodiment are formed in a horizontal plate shape having a thickness dimension of about 2 mm, and are vertically symmetrical so as to be symmetrical in the drawing. Eight steps are arranged at a pitch of 6.8 mm in the direction. See FIG. Further, the distance between the lower surface 31 of the lowermost shelf plate 15a and the upper surface 32 of the bottom of the storage container 1 is set to about 4 mm. See FIG. As a result, in the first culture container 2a and the second culture container 3a, the lower collar portions 17 and 21 provided therein are located between the upper surface 32 of the bottom of the storage container 1 and the lower surface 31 of the lowermost shelf plate 15a. It is inserted into the formed space and stored in the storage position located at the bottom of the storage container 1.
また、収納容器１の最下部に収納される培養容器２ａ、３ａよりも上方に収納される培養容器２、３は、各培養容器２、３にそれぞれ形成される上鍔部１６、２０の下面を各棚板１５の上面に当接させることで、各棚板１５によって鉛直方向に支持される。言い換えると、各培養容器２、３の左右一対の上鍔部１６、２０を左右一対の各棚板１５にそれぞれ引っ掛けることで、各培養容器２、３は各棚板１５によって懸架状態で支持される。この収納形態とすることで各棚板１５は、各培養容器２、３の下方ではなく、各培養容器２、３の側面壁１８、１９に対向する位置に位置することとなる。上記のような収納形態とすることで、収納容器１に収納された培養容器２、３の上下方向の間隔Ｘを棚板１５の厚み寸法よりも小さくすることが出来るので、収納容器１の上下方向の寸法を可及的に小さくすることが出来る。なお、本実施形態の収納容器１では、収納された培養容器２、３の上下方向の隙間の寸法は約０．５ｍｍとしている。上記したように、本実施形態の収納容器１は、各培養容器２、３を上下方向に隙間をあけて収納するので、棚段状に収納された各培養容器２、３のうち中間位置に収納された培養容器２、３だけを取り出すことが可能になる。 Further, the culture containers 2 and 3 stored above the culture containers 2a and 3a stored at the bottom of the storage container 1 are the lower surfaces of the upper flange portions 16 and 20 formed in the culture containers 2 and 3, respectively. Is brought into contact with the upper surface of each shelf board 15, and is supported in the vertical direction by each shelf board 15. In other words, by hooking the pair of left and right upper collars 16 and 20 of the culture vessels 2 and 3 on the pair of left and right shelf plates 15, the culture vessels 2 and 3 are supported by the shelf plates 15 in a suspended state. To. With this storage form, each shelf board 15 is located at a position facing the side walls 18 and 19 of each culture container 2 and 3 instead of below each culture container 2 and 3. By adopting the storage form as described above, the vertical distance X between the culture containers 2 and 3 stored in the storage container 1 can be made smaller than the thickness dimension of the shelf board 15, so that the upper and lower sides of the storage container 1 can be set. The dimension in the direction can be made as small as possible. In the storage container 1 of the present embodiment, the size of the vertical gap between the stored culture containers 2 and 3 is about 0.5 mm. As described above, since the storage container 1 of the present embodiment stores the culture containers 2 and 3 with a gap in the vertical direction, the storage container 1 is located at an intermediate position among the culture containers 2 and 3 stored in a shelf shape. Only the stored culture containers 2 and 3 can be taken out.
図８の収納容器２２は、培養容器２、３の底面を支持するように収納容器１の棚板１５の配置間隔を変更したものである。なお、各棚板１５の厚み寸法は第１の実施形態の収納容器１と同じ寸法としている。収納容器２２では、各培養容器２の上面と各棚板１５の下面との間のＸ１と各棚板１５の厚みＸ２とを加えた寸法が、上下方向に収納される培養容器２、３の上下方向の間隔Ｘ３となるため、収納容器１と同じ数の培養容器２、３を収納する形態とした場合、収納容器２２の上下方向の寸法は収納容器１と比べて増大する。本発明の発明者は、収納容器２２が、収納容器１が備える棚板１５と同じ棚板１５を備え、同じ数の第１の培養容器２を収納すると仮定して、収納容器２２の上下寸法を比較したところ、本実施形態の収納容器１の上下方向の寸法は、従来の収納容器２２の上下方向の寸法に比べて、約２０パーセント削減することが出来ることを導き出した。 In the storage container 22 of FIG. 8, the arrangement interval of the shelf board 15 of the storage container 1 is changed so as to support the bottom surfaces of the culture containers 2 and 3. The thickness of each shelf board 15 is the same as that of the storage container 1 of the first embodiment. In the storage container 22, the dimensions obtained by adding X1 between the upper surface of each culture container 2 and the lower surface of each shelf board 15 and the thickness X2 of each shelf board 15 are the dimensions of the culture containers 2 and 3 stored in the vertical direction. Since the vertical interval X3 is provided, when the same number of culture containers 2 and 3 as the storage container 1 are stored, the vertical dimension of the storage container 22 is larger than that of the storage container 1. The inventor of the present invention assumes that the storage container 22 has the same shelf board 15 as the shelf board 15 included in the storage container 1 and stores the same number of first culture containers 2, and the vertical dimensions of the storage container 22. As a result, it was derived that the vertical dimension of the storage container 1 of the present embodiment can be reduced by about 20% as compared with the vertical dimension of the conventional storage container 22.
上記第１の実施形態では上下の端部に鍔部１６、１７、２０、２１を備える培養容器２、３と、これらを収納する収納容器１について説明したが、次に、鍔部２６を一対のみ備える培養容器２４、２５とこれらを収納する収納容器２３について説明する。図１０は、本発明の第２の実施形態である収納容器２３と第１の培養容器２４、第２の培養容器２５の収納状態を示す断面図である。 In the first embodiment, the culture containers 2 and 3 having collars 16, 17, 20, and 21 at the upper and lower ends and the storage container 1 for storing these have been described. Next, a pair of collars 26 are provided. The culture containers 24 and 25 provided only with the above and the storage container 23 for storing them will be described. FIG. 10 is a cross-sectional view showing a storage state of the storage container 23, the first culture container 24, and the second culture container 25 according to the second embodiment of the present invention.
本実施形態の第１の培養容器２４は、第１の実施形態の第１の培養容器２と外形寸法は同一であり、本実施形態の第２の培養容器２５は、第１の実施形態の第２の培養容器３と外形寸法は同一である。本実施形態の第１の培養容器２４に形成される鍔部２６は、厚み寸法が２ｍｍであり、第１の培養容器２４の長手方向の側面壁から張り出すように形成されている。また、鍔部２６の下面は、第１の培養容器２４の底面から３．５ｍｍの位置になるように形成されている。本実施形態の第２の培養容器２５に形成される鍔部２６は、厚み寸法が２ｍｍであり、第１の培養容器２５の長手方向の側面壁から張り出すように形成されている。また、鍔部２６の下面は、第２の培養容器２５の底面から３．５ｍｍの位置になるように形成されている。 The first culture vessel 24 of the present embodiment has the same external dimensions as the first culture vessel 2 of the first embodiment, and the second culture vessel 25 of the present embodiment has the same external dimensions as that of the first embodiment. The external dimensions are the same as those of the second culture vessel 3. The collar portion 26 formed in the first culture vessel 24 of the present embodiment has a thickness dimension of 2 mm and is formed so as to project from the side wall in the longitudinal direction of the first culture vessel 24. The lower surface of the collar portion 26 is formed so as to be at a position 3.5 mm from the bottom surface of the first culture vessel 24. The collar portion 26 formed in the second culture vessel 25 of the present embodiment has a thickness dimension of 2 mm and is formed so as to project from the side wall in the longitudinal direction of the first culture vessel 25. The lower surface of the collar portion 26 is formed so as to be at a position 3.5 mm from the bottom surface of the second culture container 25.
上記第２の実施形態である第１の培養容器２４、及び第２の培養容器２５を収納する第２の収納容器２３は、第１の収納容器１と同様に培養容器２４、２５を支持する左右一対の棚板２７が上下方向に間隔を空けて八段形成されている。本実施形態の収納容器２３に形成される最下段の棚板２７ａは、棚板２７ａの上面と収納容器２３の底部上面との距離は約３．５ｍｍに設定されている。また、最下段の棚板２７ａの上面と下から２段目の棚板２７ｂの上面との距離は約６．２ｍｍに設定されており、八段目の各棚板２７はいずれも約６．８ｍｍの距離となるように各棚板２７は配置されている。上記構成の収納容器２３の各棚板に、第１の培養容器２４を４個、もしくは第２の培養容器２５を８個収納した場合、各培養容器２４、２５の上下方向のクリアランスは約０．５ｍｍとなり、第１の実施形態の収納容器１のクリアランスとほぼ同一のクリアランスを達成することが出来る。 The first culture container 24 and the second storage container 23 for storing the second culture container 25 according to the second embodiment support the culture containers 24 and 25 in the same manner as the first storage container 1. A pair of left and right shelf boards 27 are formed in eight stages with an interval in the vertical direction. In the lowermost shelf board 27a formed in the storage container 23 of the present embodiment, the distance between the upper surface of the shelf board 27a and the bottom upper surface of the storage container 23 is set to about 3.5 mm. Further, the distance between the upper surface of the lowermost shelf board 27a and the upper surface of the second shelf board 27b from the bottom is set to about 6.2 mm, and each of the eighth shelf boards 27 is about 6. Each shelf board 27 is arranged so as to have a distance of 8 mm. When four first culture containers 24 or eight second culture containers 25 are stored in each shelf plate of the storage container 23 having the above configuration, the vertical clearance of each of the culture containers 24 and 25 is about 0. The thickness is 5.5 mm, and a clearance substantially the same as the clearance of the storage container 1 of the first embodiment can be achieved.
本発明の収納容器１、２３に培養容器２、３、２４、２５を収納する作業は、内部を清浄な雰囲気に維持されたクリーンベンチやアイソレータ内で行われる。作業者は培養容器２、３、２４、２５に培地の供給、細胞の播種を行った後、収納容器１、２３の蓋体６を開け、本体５の内部に差し込むように収納する。そして、蓋体６を本体５に嵌合させることで収納容器１、２３の内部を密封した後、所定のインキュベータは検査装置へと移動させる。蓋体６によって収納容器１、２３の内部を密封した後は、収納容器１、２３を一般大気環境に持ち出しても浮遊している塵埃や雑菌が収納容器１、２３の内部に侵入してくることは無い。 The work of storing the culture containers 2, 3, 24, and 25 in the storage containers 1 and 23 of the present invention is performed in a clean bench or an isolator in which the inside is maintained in a clean atmosphere. After supplying the culture medium to the culture containers 2, 3, 24, and 25 and seeding the cells, the worker opens the lid 6 of the storage containers 1 and 23 and stores the cells so as to be inserted into the main body 5. Then, after the inside of the storage containers 1 and 23 is sealed by fitting the lid 6 to the main body 5, the predetermined incubator is moved to the inspection device. After the inside of the storage containers 1 and 23 is sealed by the lid 6, floating dust and germs invade the inside of the storage containers 1 and 23 even if the storage containers 1 and 23 are taken out to the general air environment. There is no such thing.
以上のように、本発明を各実施の形態により詳しく説明したが、本発明のこれら実施形態に限定されるものではなく、その要旨を逸脱しない範囲で変更可能である。
As described above, the present invention has been described in more detail with respect to each embodiment, but the present invention is not limited to these embodiments of the present invention, and can be changed without departing from the gist thereof.
Claims (7)
前記開口を気密に閉鎖可能な蓋体とで構成される収納容器であって、
前記本体は、
前記本体に形成された通気口をガス流通可能に閉鎖するフィルタ部材と、
前記本体の内部空間において、
鉛直方向に所定の間隔を空けて配置される複数の棚板と、を備え、
前記複数の棚板は、前記培養容器の側面壁に突出して形成される一対の鍔部とそれぞれ係合することで前記培養容器を鉛直方向に支持することを特徴とする収納容器。 The main body with an opening through which the culture vessel can pass,
A storage container composed of a lid that can airtightly close the opening.
The main body
A filter member that closes the vent formed in the main body so that gas can flow.
In the internal space of the main body
With a plurality of shelves arranged at predetermined intervals in the vertical direction,
The storage container is characterized in that the plurality of shelves support the culture container in the vertical direction by engaging with a pair of collars formed so as to project from the side wall of the culture container.
支持対象の前記培養容器の底部よりも上方であり、
且つ、前記培養容器の頂部よりも下方に位置する
ことを特徴とする請求項１に記載の収納容器。 The shelf board is used when the culture container is stored in the main body.
Above the bottom of the culture vessel to be supported
The storage container according to claim 1, wherein the storage container is located below the top of the culture container.
ことを特徴とする請求項１もしくは請求項２に記載の収納容器。 The storage container according to claim 1 or 2, wherein the depth dimension of the main body of the storage container is smaller than the depth dimension of the culture container.
所定の高さ寸法を有する第１の培養容器と、
前記第１の培養容器の前記高さ寸法の約１／２の高さ寸法を有する第２の培養容器の
いずれも収納可能であることを特徴とする請求項１から請求項３のいずれか一項に記載の収納容器。 The storage container is
A first culture vessel having a predetermined height dimension and
Any one of claims 1 to 3, characterized in that any of the second culture vessels having a height dimension of about 1/2 of the height dimension of the first culture vessel can be stored. The storage container described in the section.
The storage container according to any one of claims 1 to 6, wherein the lid is made of polyethylene.
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