JP2020066602A - Body cooling agent and method of producing body cooling liquid - Google Patents

Abstract

To provide, e.g., a body cooling agent capable of cooling a body while inhibiting reduction in a blood flow, and a method of producing a body cooling liquid including a step of bringing the body cooling agent into contact with a liquid.SOLUTION: Ice (preferably COhydrate) containing 3 wt.% or more of COis used. A body cooling liquid prepared at the time of use by bringing the ice (preferably COhydrate) containing 3 wt.% or more of COinto contact with a liquid is applied to the skin so that a blood flow in the skin can be promoted.SELECTED DRAWING: None

Description

  The present invention relates to a body cooling agent and a method for producing a body cooling solution, and more specifically, a body cooling agent for preparing a body cooling solution for application to the skin at the time of use, and the body cooling solution. Manufacturing method, etc.

Icing is a first aid for injury or illness; prevention of injury during exercise; reduction of muscle pain and fatigue accumulation after exercise; hemostasis; Is to cool to. These uses of icing are based on the following (a)-(d) physiological effects of icing.
(A) Rapidly contracts blood vessels to suppress internal bleeding and inflammation.
(B) By suppressing blood flow and metabolism in the fatigued and damaged areas, the expansion of fatigue and damage from the aforementioned areas to surrounding tissues is suppressed.
(C) Paralyze the pain nerve.
(D) The activity of intramuscular cells is reduced.

  The effectiveness of icing has become more widely recognized than before, and sprays for icing are widely marketed. However, when the icing is performed by the conventional method, the blood flow remains low for a relatively long time even after the icing is completed, so that a fatigue substance (lactic acid) is accumulated and exercise performance is deteriorated. Therefore, when icing is performed by the conventional method, a method of warming the iced portion after the icing is performed is used. However, this method has a drawback that it is difficult to determine how much warming should be performed, and the effect of icing is significantly reduced if the warming is excessive. Under such circumstances, there has been a demand for an icing method in which these drawbacks are improved.

By the way, a substance called CO ₂ hydrate (carbon dioxide hydrate) is known as a type of ice having a high CO ₂ content. The CO ₂ hydrate refers to an inclusion compound in which carbon dioxide molecules are confined in the voids of a crystal of water molecules. A water molecule forming a crystal is called a "host molecule", and a molecule of water molecule confined in an empty space of a crystal is called a "guest molecule" or "guest substance". When the CO ₂ hydrate melts, it decomposes into CO ₂ (carbon dioxide) and water, so that CO ₂ is generated during melting. CO ₂ hydrate can be produced by subjecting CO ₂ and water to a condition of low temperature and high CO ₂ partial pressure, for example, at a certain temperature, and CO ₂ hydrate at that temperature. It can be produced under conditions including a higher CO ₂ partial pressure than the equilibrium pressure of the rate (hereinafter, also referred to as “CO ₂ hydrate production condition”). CO ₂ content of the CO ₂ hydrate, depending on the preparation of CO ₂ hydrate, can be on the order of about 3 to 28 wt%, CO ₂ content of the carbonated water (about 0.5 wt% ) Is significantly higher than

As application of CO ₂ hydrate, the addition of CO ₂ hydrate beverages, it is known to mix. For example, in Patent Document 1, a carbonated beverage is produced by adding carbonic acid to the beverage by mixing CO ₂ hydrate with the beverage, and in Patent Document 2, the CO ₂ hydrate is covered with ice. It is disclosed that the carbonated replenishing medium thus formed is added to the beverage to cool the slimy beverage and to supplement the degassed beverage with carbon dioxide gas. Further, Patent Document 3, fresh food, dairy products, any one of the cold object of confectionery and fresh flowers, a method of cold using a CO ₂ hydrate, CO ₂ hydrate and the cold insulating target There is disclosed a method of keeping an object to be kept cold by accommodating the object in a container that can be sealed without contacting it. Further, Patent Document 4 uses an oxygen hydrate (O ₂ hydrate) to cool a body part of a bather, a beverage or the like for the bather, to prevent hot flashes, and to realize a comfortable bathing environment. Is disclosed.

However, it has not been known that cooling the body with ice having a CO ₂ content of 3% by weight or more, such as CO ₂ hydrate, can cool the body while suppressing a decrease in blood flow. .

JP, 2005-224146, A Japanese Patent No. 4969683 Japanese Patent No. 4500566 JP, 2007-319280, A

  An object of the present invention is to provide a body cooling agent capable of cooling the body while suppressing a decrease in blood flow, a method for producing a body cooling solution including a step of bringing the body cooling agent into contact with a liquid, and the like. Especially.

The inventors of the present invention have made diligent studies to solve the above-mentioned problems, and bring ice (preferably CO ₂ hydrate) having a CO ₂ content of 3% by weight or more into contact with the liquid (preferably contained in the liquid). It was found that when the prepared low temperature liquid is applied to the skin of the body of an animal, the body can be cooled while suppressing the decrease in blood flow in the skin, and the present invention has been completed.

That is, the present invention is
(1) A body cooling agent containing ice having a CO ₂ content of 3% by weight or more;
(2) The body cooling agent according to (1) above, wherein the ice having a CO ₂ content of 3% by weight or more is CO ₂ hydrate;
(3) The ice having a CO ₂ content of 3% by weight or more is an ice having a maximum length of 3 mm or more and a CO ₂ content of 3% by weight or more. (1) or (2) Body coolant;
(4) The ice having a CO ₂ content of 3% by weight or more is treated with water so that the concentration of the ultrafine bubbles measured by the following measuring method R1 is 5 million / mL or more. Body cooling agent according to any one of (1) to (3) above, characterized in that it is ice that can be generated therein.
(Measurement method R1)
To water at 25 ° C, 300 mg / mL of ice having a CO ₂ content of 3% by weight or more and -80 to 0 ° C is added to contain ice having a CO ₂ content of 3% by weight or more. After making ice water at 0 to 2 ° C., the concentration (pieces / mL) of ultrafine bubbles in the ice water is measured by Malvern Nanosite NS300;
(5) The body cooling agent according to any one of (1) to (4) above, wherein the ice having a CO ₂ content of 3% by weight or more is a consolidated CO ₂ hydrate; or
(6) The body cooling agent according to any one of (1) to (5) above, which is for preparing a body cooling liquid for application to the skin at the time of use;
Regarding

Further, the present invention is
(7) A method for producing a body cooling liquid for application to the skin, which comprises a step of bringing the body cooling agent according to any one of (1) to (6) into contact with a liquid; or
(8) For the body to be applied to the skin, which is a liquid containing 200 ppm or more of carbonic acid, and has an ultrafine bubble concentration of 5 million cells / mL or more when measured by the following measuring method R1 Cooling liquid;
(Measurement method R1)
To water at 25 ° C, 300 mg / mL of ice having a CO ₂ content of 3% by weight or more and -80 to 0 ° C is added to contain ice having a CO ₂ content of 3% by weight or more. After making ice water at 0 to 2 ° C., the concentration (pieces / mL) of ultrafine bubbles in the ice water is measured by Malvern Nanosite NS300;
Regarding

  According to the present invention, there is provided a body cooling agent capable of cooling the body while suppressing a decrease in blood flow, a method for producing a body cooling solution including a step of bringing the body cooling agent into contact with a liquid, and the like. be able to.

It is a figure which shows the particle size distribution of bubbles and number density | concentration (pieces / mL) in the ice water containing CO ₂ hydrate. The horizontal axis of the graph represents the particle size (nm) of bubbles, and the vertical axis represents the number concentration of bubbles (cells / mL). It is a figure which shows the result of having measured the blood flow volume of the tail of a rat. The blood flow rate of the rat tail was measured for 30 minutes while the rat tail was immersed in each sample ice water, and for 30 minutes after the completion of the immersion. The horizontal axis of the graph represents the elapsed time (minutes) from the start of immersion of the tail in each sample ice water, and the vertical axis of the graph represents the average value of blood flow for 5 minutes before the start of immersion as 100%. , Represents the relative value (%) of blood flow. The relative value (%) of blood flow shown in the graph was calculated using the average value of blood flow every 5 minutes. For example, the relative value of the blood flow 5 minutes after the start of the immersion was calculated using the average value of the blood flow 5 minutes after the start of the immersion.

The present invention is
[1] The body coolant according to claim 1, wherein the ice having a CO ₂ content of 3% by weight or more (hereinafter, also referred to as “CO ₂ high-content ice”) is CO ₂ hydrate. Also referred to as "body cooling agent of the present invention.";
[2] A method for producing a body cooling liquid for application to the skin, which comprises a step of bringing the body cooling agent of the present invention into contact with a liquid (hereinafter, also referred to as the “production method of the present invention”);
[3] A liquid containing 200 ppm or more of carbonic acid, and the concentration of ultrafine bubbles when measured by the measurement method R1 (or R2) described later is 5 million / mL or more, and applied to the skin. Body cooling liquid (hereinafter, also referred to as "body cooling liquid of the present invention");
[4] Cooling the body of an animal, which comprises the step of applying a high CO ₂ content ice (preferably CO ₂ hydrate), the body cooling agent or the body cooling solution of the present invention to the whole or local skin of the animal. Method (hereinafter, also referred to as "body cooling method of the present invention");
Etc. are included. In the present specification, the “agent” can be restated as “substance” or “composition”.

1. <Body cooling agent of the present invention>
(Ice with a CO ₂ content of 3% by weight or more)
The body cooling agent of the present invention is not particularly limited as long as it contains ice with a CO ₂ content of 3% by weight or more (“ice with high CO ₂ content”). Such CO ₂ high content ice, CO ₂ may be a CO ₂ high content of ice is not a hydrate (preferably, blood flow maintenance effect) higher blood flow decrease suppression effect from the viewpoint of obtaining, CO ₂ Hyde A rate is preferable, and a consolidated CO ₂ hydrate is more preferable. Further, as the CO ₂ high content of ice in the present invention, CO ₂ without using a hydrate, CO ₂ may be used CO ₂ high content of ice is not a hydrate, not the CO ₂ hydrate CO ₂ high content of ice the without, may be used CO ₂ hydrate, and CO ₂ high content of ice is not a CO ₂ hydrate, it may be used in combination of CO ₂ hydrate. Moreover, as CO ₂ hydrate, without the compaction CO ₂ hydrate, it may be used CO ₂ hydrate which is not compacted, without using the CO ₂ hydrate which is not consolidated, compacted CO may be used ₂ hydrate, it may be used in combination of CO ₂ hydrate and compaction CO ₂ hydrate which is not compacted.

CO ₂ hydrate is a solid clathrate compound in which carbon dioxide molecules are confined in the voids of water molecule crystals. CO ₂ hydrate is usually an ice-like crystalline substance, and when it is placed under, for example, standard atmospheric pressure conditions and temperature conditions such that ice melts, it releases CO ₂ while melting. As described above, CO ₂ high content of ice for use in the present invention, CO ₂ than CO ₂ high content of ice is not a hydrate, preferably a CO ₂ hydrate, to be compacted CO ₂ hydrate More preferable. The reason is that when the body cooling agent of the present invention is brought into contact with a liquid, CO ₂ bubbles (preferably ultra fine bubbles) can be obtained at a higher concentration, and as a result, a higher blood flow lowering suppression effect is obtained. This is because it is considered that (preferably, blood flow maintaining effect) can be obtained. "Ultra fine bubbles" are fine bubbles having a diameter of 1000 nm or less in a solvent such as water under normal pressure. Such ultrafine bubbles have (1) a significantly large interfacial surface area of the bubbles, (2) a large internal pressure of the bubbles, and (3) a high gas dissolution efficiency, as compared with ordinary bubbles having a diameter of 1 mm or more. , (4) Slow bubble rising speed, and other excellent characteristics. An ultrafine bubble generator is usually essential for the production of such ultrafine bubbles. However, when ice with a high CO ₂ content (preferably CO ₂ hydrate, more preferably consolidated CO ₂ hydrate) is used, Fine bubbles of CO ₂ (preferably ultra fine bubbles) can be easily generated without using a fine bubble generator.

The CO ₂ -rich ice in the present invention is the concentration (pieces / mL) of ultrafine bubbles (preferably CO ₂ ultrafine bubbles) when measured by the following measuring method R1, and preferably 5 million pieces. / ML or more, more preferably 10 million pieces / mL or more, further preferably 20 million pieces / mL or more, more preferably 25 million pieces / mL or more, further preferably 30 million pieces / mL or more, More preferably 35 million pieces / mL or more, further preferably 50 million pieces / mL or more, more preferably 75 million pieces / mL or more, further preferably 100 million pieces / mL or more, more preferably 150 million cells / mL or more, more preferably 200 million / mL or more, more 250 million / mL or more ultra-fine bubbles (preferably, the CO ₂ Ultrafine bubbles) preferably in water CO ₂ high content of ice that can be generated on can be preferably mentioned.
(Measurement method R1)
To water at 25 ° C, 300 mg / mL of ice having a CO ₂ content of 3% by weight or more and -80 to 0 ° C is added to contain ice having a CO ₂ content of 3% by weight or more. After making ice water at 0 to 2 ° C., the concentration of ultrafine bubbles (in pieces / mL) in the ice water is measured by Malvern Nanosite NS300.

The upper limit of the concentration of ultrafine bubbles (preferably, ultrafine bubbles of CO ₂ ) that can be generated in water by the CO ₂ -rich ice in the present invention is not particularly limited, but the measurement method R1 described above is used. The concentration of ultrafine bubbles when measured in 1 is, for example, 10 billion / mL or less and 1 billion / mL or less.

The more specific concentration of the ultra fine bubbles (preferably, CO ₂ ultra fine bubbles) that can be generated in water by the CO ₂ -rich ice in the present invention is 5 million to 10 billion / mL, 5 million to 1 billion pieces / mL, 10 million to 10 billion pieces / mL, 10 million to 1 billion pieces / mL, 20 million to 10 billion pieces / mL, 20 million to 1 billion pieces / mL mL, 25 million to 10 billion / mL, 25 million to 1 billion / mL, 30 million to 10 billion / mL, 30 million to 1 billion / mL, 35 million Million to 10 billion / mL, 35 to 1 billion / mL, 50 to 10 billion / mL, 50 to 1 billion / mL, 75 to 10 billion / mL mL, 75 million to 1 billion pieces / mL, 100 million to 10 billion pieces / mL, 100 million to 1 billion pieces / mL, 150 million to 10 billion pieces / mL, 105 million 10,000 to 1 billion / mL, 200 to 10 billion / mL, 200 to 1 billion / mL, 250 to 10 billion / mL, 250 to 1 billion / mL, etc. Is mentioned.

The CO ₂ content of CO ₂ high content of ice in the present invention (preferably CO ₂ hydrate), is not particularly limited as long as it is 3 wt% or more, bubbles CO ₂ (preferably ultra fine bubble) higher the From the viewpoint of obtaining a higher blood flow reduction suppressing effect (preferably a blood flow maintaining effect) when obtained at a concentration, it is preferably 5% by weight or more, more preferably 7% by weight or more, still more preferably 10% by weight or more, It is preferably 13% by weight or more, more preferably 16% by weight or more, and further preferably 18% by weight or more. The upper limit value is not particularly limited, but may be 30% by weight, 28% by weight, 26% by weight, or 24% by weight. More specific CO ₂ content of CO ₂ high content of ice (preferably CO ₂ hydrate), 5 to 30 wt%, 7-30 wt%, 10 to 30 wt%, 13 to 30 wt%, 16 -30 wt%, 18-30 wt%, 5-28 wt%, 7-28 wt%, 10-28 wt%, 13-28 wt%, 16-28 wt%, 18-28 wt%, 5-26 %, 7 to 26% by weight, 10 to 26% by weight, 13 to 26% by weight, 16 to 26% by weight, 18 to 26% by weight, and the like.

CO ₂ content of the CO ₂ high content of ice in the present invention can be adjusted by such as "level of CO ₂ partial pressure" in the production of the CO ₂ high content of ice in the present invention, for example, CO ₂ partial pressure When it is increased, the CO ₂ content of the CO ₂ -rich ice can be increased. Further, when the CO ₂ -rich ice is CO ₂ hydrate, “high or low CO ₂ partial pressure”, “degree of dehydration treatment”, and “whether or not compression treatment is performed” when the CO ₂ hydrate is produced. The “CO ₂ content rate of the CO ₂ hydrate” can be adjusted by, for example, “high or low compression pressure when performing compression processing”. For example, when producing a CO ₂ hydrate, "increase the CO ₂ partial pressure", "increase the degree of dehydration treatment", "perform a compression treatment", "increase the consolidation pressure in the compression treatment". And, the CO ₂ content of the CO ₂ hydrate can be increased. Incidentally, when the CO ₂ high content of ice CO ₂ hydrate and the like are melted, CO ₂ contained in the CO ₂ high content of ice, such as the CO ₂ hydrate is released, so that amount of the weight is decreased, CO CO ₂ content of the CO ₂ high content of ice, such as ₂ hydrate, for example, the CO ₂ high content of ice CO ₂ hydrate and the like from the weight change when melted at room temperature, using the following equation (1) It can be calculated.
(CO ₂ content) = (sample weight before melting−sample weight after melting) / sample weight before melting) ... Formula (1)

Moreover, CO ₂ high content of ice containing the body cooling agent of the present invention (preferably CO ₂ hydrate) are all of, but is preferably a CO ₂ content of more than 3 wt%, the effect of the present invention In the range where (the effect of cooling the body while suppressing the decrease in blood flow is obtained), ice or CO ₂ hydrate having a CO ₂ content of less than 3% by weight may be contained. The relative CO ₂ high content of ice containing the body cooling agent of the present invention (preferably CO ₂ hydrate), the proportion of ice or CO ₂ hydrate of CO ₂ content is less than 3 wt% (wt%), 10 The amount is preferably not more than 5% by weight, preferably not more than 5% by weight, more preferably not more than 3% by weight, still more preferably not more than 1% by weight.

The shape of the CO ₂ high content of ice (preferably CO ₂ hydrate) in the present invention can be suitably set, for example, substantially spherical; substantially ellipsoidal; substantially polyhedral shape such as a substantially rectangular parallelepiped shape; or, they And the like; Moreover, CO ₂ high content of ice in the present invention (preferably CO ₂ hydrate) is, CO ₂ high content of ice (preferably CO ₂ hydrate) fragments of various shapes obtained by appropriately crushed mass (mass ) May be sufficient.

The size of the CO ₂ high content of ice in the present invention (preferably CO ₂ hydrate) is not particularly limited, it can be appropriately set. As the maximum length limit of the CO ₂ high content of ice in the present invention (preferably CO ₂ hydrate), preferably 3mm or more, more preferably 5mm or more, more preferably 7mm or more, more preferably it includes more than 10 mm, maximum The upper limit of the length is 150 mm or less, 100 mm or less, 80 mm or less, 60 mm or less, and more specifically, 3 mm or more and 150 mm or less, 3 mm or more 100 mm or less, 3 mm or more 80 mm or less, 3 mm or more 60 mm or less, or 5 mm or more 150 mm or less, 5 mm or more and 100 mm or less, 5 mm or more and 80 mm or less, 5 mm or more and 60 mm or less, 10 mm or more and 150 mm or less, 10 mm or more and 100 mm or less, 10 mm or more and 80 mm or less, 10 mm or more and 60 mm or less, and the like.

In the present specification, the “maximum length of ice with high CO ₂ content” is the longest line segment that connects two points on the surface of the ice cube with high CO ₂ content and that passes through the center of gravity of the ice cube. Means the length of. In addition, when the CO ₂ -rich ice has, for example, a substantially ellipsoidal shape, the maximum length represents a major axis (longest diameter), and when the ice has a substantially spherical shape, the maximum length represents a diameter and has a substantially rectangular parallelepiped shape. In some cases, it represents the length of the longest diagonal line. Further, in the present specification, the "CO ₂ minimum length of the high-containing ice" is, CO ₂ high content of ice (preferably CO ₂ hydrate) signed two points of the surface of the mass, and the center of gravity of the mass It means the length of the shortest line segment among the line segments that pass. Such maximum length and minimum length, can either be measured by using a commercially available image analysis type particle size distribution measuring apparatus, CO ₂ high content of ice (preferably CO ₂ hydrate) is measured by applying a ruler mass You can also

As a preferred embodiment of the CO ₂ high content of ice in the present invention (preferably CO ₂ hydrate), aspect ratio (maximum length / minimum length) is preferably in the range of 1 to 5, more preferably in the range of 1-4 , And more preferably, a CO ₂ -rich ice (preferably CO ₂ hydrate) in the range of 1 to 3 is mentioned.

CO ₂ high content size of ice (preferably CO ₂ hydrate) can be adjusted by the following method. For example, the maximum length of the CO ₂ high content of ice is not a CO ₂ hydrate, or adjust the maximum length of the mold in the production of such a CO ₂ high content of ice, when crushing the CO ₂ high content of ice after production It can be adjusted by adjusting the degree of crushing. The maximum length of the CO ₂ hydrate, adjusting the degree of crushing at the time of crushing and adjusting the maximum length of the mold to be used for compression molding of CO ₂ hydrate, the CO ₂ hydrate after compression molding It can be adjusted by The minimum length can be adjusted by adjusting the minimum length of the mold or adjusting the degree of crushing the produced CO ₂ -rich ice.

The method for producing the CO ₂ -rich ice in the present invention is not particularly limited as long as the CO ₂ -rich ice can be produced. As a method for producing CO ₂ hydrate in CO ₂ high content of ice is not a method of freezing the raw water while blowing CO ₂ into the raw material water under conditions that do not meet the CO ₂ hydrate formation conditions and the like. Further, as a method for producing a CO ₂ hydrate, and the gas-liquid agitation method for agitating the raw water while blowing CO ₂ into the raw material water under conditions satisfying the CO ₂ hydrate formation conditions satisfying the CO ₂ hydrate formation conditions An ordinary method such as a water spray method in which raw material water is sprayed into CO ₂ under the conditions can be used. The CO ₂ hydrate produced by these methods is usually in the form of a slurry in which fine particles of CO ₂ hydrate are mixed with unreacted water. Therefore, in order to increase the concentration of CO ₂ hydrate, dehydration is performed. Treatment is preferred. The CO ₂ hydrate having a relatively low water content due to the dehydration treatment (that is, a relatively high concentration of CO ₂ hydrate) can be compression-molded into a certain shape (for example, a spherical shape or a rectangular parallelepiped shape) by a pellet molding machine. preferable. The compression-molded CO ₂ hydrate can be preferably used as one kind of the consolidated CO ₂ hydrate in the present invention. The compression-molded CO ₂ hydrate may be used as it is in the present invention, or may be further crushed if necessary. Incidentally, CO ₂ as a method for producing a hydrate, as described above, a method using a raw water are used relatively extensively, water CO ₂ instead of fine ice (material ice) of (raw water) It is also possible to use a method of producing CO ₂ hydrate by reacting with CO ₂ hydrate under a condition of low temperature and low CO ₂ partial pressure.

"CO ₂ hydrate formation conditions" above, as described above, the equilibrium pressure than CO ₂ partial pressure of CO ₂ hydrate at a temperature (CO ₂ pressure) is high condition. The above-mentioned “condition where the CO ₂ partial pressure is higher than the equilibrium pressure of CO ₂ hydrate” is described in J. Chem. Eng. Data (1991) 36, 68-71, Figure 2. and J. Chem. Eng. Data. (2008), 53, 2182-2188, CO ₂ hydrate equilibrium pressure curves (eg CO ₂ pressure on the vertical axis and temperature on the horizontal axis) disclosed in Figure 7. As a condition of the combination of the CO ₂ pressure and the temperature in the region on the high pressure side (in the equilibrium pressure curve of the CO ₂ hydrate, for example, when the vertical axis represents the CO ₂ pressure and the horizontal axis represents the temperature, above the curve) expressed. As a specific example of the CO ₂ hydrate generation condition, a condition of a combination of “within a range of −20 to 4 ° C.” and “a carbon dioxide pressure within a range of 1.8 to 4 MPa” and a range of “−20 to −4 ° C. The conditions of the combination of "inside" and "within the range of carbon dioxide pressure of 1.3 to 1.8 MPa" are mentioned.

The content of the CO ₂ -rich ice (preferably CO ₂ hydrate) in the body cooling agent of the present invention is not particularly limited, but is, for example, in the range of 5 to 100% by weight, preferably in the range of 30 to 100% by weight. Of these, the range of 50 to 100% by weight is more preferable, and the range of 70 to 100% by weight is more preferable.

In the present invention, “consolidated CO ₂ hydrate” means that the CO ₂ hydrate rate is 40 to 90% (preferably 50 to 90%, more preferably 60 to 90%, further preferably 70 to 90%, more preferably Means a CO ₂ hydrate of 80 to 90%). The CO ₂ hydrate rate means the ratio of the weight of CO ₂ hydrate relative to the weight of CO ₂ hydrate mass (%). The CO ₂ hydrate rate can be calculated by the following equation (2).
CO ₂ hydrate rate (%) = {(sample weight before melting−sample weight after melting) + (sample weight before melting−sample weight after melting) ÷ 44 × 5.75 × 18} × 100 ÷ melting Previous sample weight ... Formula (2)
Formula (2) will be described below. (Sample weight before melting−Sample weight after melting) is the weight of CO ₂ gas contained. The amount of water required for inclusion of CO ₂ gas as a hydrate is calculated by using a theoretical hydration number of 5.75, a molecular weight of CO ₂ of 44, and a molecular weight of 18 of water, and other water constitutes a hydrate. Not considered as adhered water.

As a suitable consolidated CO ₂ hydrate in the present invention, the concentration (units / mL) of ultrafine bubbles when measured by the above-mentioned measurement method R1 is 50 million units / mL or more, more preferably 75,500 units. 10,000 pieces / mL or more, more preferably 100 million pieces / mL or more, more preferably 150 million pieces / mL or more, further preferably 200 million pieces / mL or more, more preferably 250 million pieces / mL CO ₂ hydrate that can generate the above ultra fine bubbles in water can be mentioned. Moreover, as a suitable CO ₂ content rate of the consolidated CO ₂ hydrate in the present invention, ultrafine bubbles can be obtained at a higher concentration, and a higher blood flow reduction suppressing effect (preferably, blood flow maintaining effect) can be obtained. From the viewpoint, it is preferably 7% by weight or more, more preferably 10% by weight or more, further preferably 13% by weight or more, more preferably 16% by weight or more, and further preferably 18% by weight or more. The upper limit value is not particularly limited, but examples thereof include 30% by weight, 28% by weight, 26% by weight, and 24% by weight. More specific CO ₂ content of the preferred consolidated CO ₂ hydrate in the present invention is 7 to 30% by weight, 10 to 30% by weight, 13 to 30% by weight, 16 to 30% by weight, 18 to 30. % By weight, 7 to 28% by weight, 10 to 28% by weight, 13 to 28% by weight, 16 to 28% by weight, 18 to 28% by weight, 7 to 26% by weight, 10 to 26% by weight, 13 to 26% by weight , 16 to 26% by weight, 18 to 26% by weight, and the like.

The method for producing the consolidated CO ₂ hydrate in the present invention is not particularly limited, but the following production methods can be preferably mentioned, for example.
And gas-liquid agitation method for agitating the raw water while blowing CO ₂ under conditions satisfying the CO ₂ hydrate formation conditions feed water, spraying the raw material water in CO ₂ under conditions satisfying the CO ₂ hydrate formation conditions A conventional method such as a water spray method can be used. The CO ₂ hydrate produced by these methods is usually in the form of slurry in which fine particles of CO ₂ hydrate are mixed with unreacted water. Condensed CO ₂ hydrate can be produced by performing dehydration treatment and compression treatment on such a slurry. The dehydration treatment and compression treatment of the slurry containing the CO ₂ hydrate particles and water include, for example, a dehydration treatment and a compression treatment of the CO ₂ hydrate particles after the slurry is dehydrated. It is possible to perform the dehydration treatment and the compression treatment at the same time, for example, by performing the compression treatment on the slurry under the condition that the water in the slurry can be discharged. Therefore, from the viewpoint of obtaining a higher blood flow reduction suppressing effect (preferably a blood flow maintaining effect), it is preferable to perform the dehydration treatment and the compression treatment at the same time, and above all, the dehydration treatment and the compression treatment under the CO ₂ hydrate formation condition. It is more preferable to carry out simultaneously. The compression treatment of the CO ₂ hydrate particles and the compression treatment of the slurry can be performed using a commercially available compaction molding machine or the like. Examples of the pressure during the compression treatment include 1 to 100 Mpa, 1 to 50 Mpa, 1 to 30 Mpa, 1 to 15 Mpa, 1 to 10 Mpa, 2.5 to 10 Mpa, and 2.5 to 9 Mpa. Note that the above-described slurry, when a sufficient dehydration treatment, CO ₂ hydrate rate is usually about becomes 40%, sufficient compression performed when CO ₂ hydrate the dehydrated treatment with 2.5 MPa CO ₂ hydrate particles The rate is usually about 60%, and if the CO ₂ hydrate particles are compressed at 9 Mpa after the dehydration treatment, the CO ₂ hydrate rate is usually about 90%.

The CO ₂ -rich ice (preferably CO ₂ hydrate) in the body cooling agent of the present invention is a CO ₂ -rich ice consisting of CO _{2 and} ice (preferably CO ₂ hydrate) (hereinafter, referred to as “containing optional components”). CO ₂ high content ice (preferably CO ₂ hydrate) ”), but a CO ₂ high content ice (preferably CO) further containing an optional component depending on the use of the body cooling agent. ₂ hydrate). Further, the body cooling agent of the present invention, "CO ₂ high content of ice containing no optional components (preferably CO ₂ hydrate)", or, CO ₂ high content of ice containing "Optional component (preferably CO ₂ Hyde Rate) ”alone, or in addition to these CO ₂ -rich ice (preferably CO ₂ hydrate), it may further contain optional components.

When the body cooling agent of the present invention contains CO ₂ -rich ice other than CO ₂ hydrate, the body cooling agent of the present invention should be kept at a temperature and a pressure at which the ice does not melt during distribution and storage. Is preferred. Examples of such temperature and pressure include conditions of 0 ° C. or lower at normal pressure (for example, 1 atm). On the other hand, depending on the production method of CO ₂ hydrate and the like, there are some that are excellent in storage stability and stability. Therefore, if the body coolant of the present invention contains a CO ₂ hydrate as a CO ₂ high content of ice, the body coolant according the present invention, when the distribution or storage, room temperature (5 to 35 ° C.), atmospheric pressure The body cooling agent of the present invention may be held at (for example, 1 atm), but from the viewpoint of keeping the body cooling agent of the present invention more stable for a longer period of time, the body cooling agent of the present invention may be stored at “low temperature” during distribution or storage. It is preferable to hold under "conditions", "high pressure conditions", or "low temperature conditions and high pressure conditions". From the viewpoint of the ease of holding, it is preferable to hold them under "low temperature conditions", and it is more preferable to hold them under "low temperature conditions" under normal pressure (for example, 1 atm).

  The upper limit temperature under the "low temperature condition" is 10 ° C or lower, preferably 5 ° C or lower, more preferably 0 ° C or lower, further preferably -5 ° C or lower, more preferably -10 ° C or lower, and further preferably- 15 degreeC or less, More preferably, it is -20 degreeC, More preferably, it is -25 degreeC, As a minimum temperature under said "low temperature conditions", -273 degreeC or more, -80 degreeC or more, -50 degreeC or more,-. 40 degreeC or more, -30 degreeC or more, etc. are mentioned.

  The lower limit pressure under the “high pressure condition” is 1.036 atm or more, preferably 1.135 atm or more, more preferably 1.283 atm or more, still more preferably 1.480 atm or more. The upper limit pressure under "high pressure conditions" includes 14.80 atm or less, 11.84 atm or less, 9.869 atm or less, 7.895 atm or less, 4.935 atm or less.

  The body coolant of the present invention may be contained in a container. The shape and material of the container are not particularly limited, and examples thereof include a plastic bottle container.

The body cooling agent of the present invention has an ultrafine bubble concentration (pieces / mL) of preferably 5 million pieces / mL or more, more preferably 10 million pieces / mL, when measured by the following measuring method R2. Or more, more preferably 20 million pieces / mL or more, more preferably 25 million pieces / mL or more, further preferably 30 million pieces / mL or more, more preferably 35 million pieces / mL or more, More preferably 50 million pieces / mL or more, more preferably 75 million pieces / mL or more, further preferably 100 million pieces / mL or more, more preferably 150 million pieces / mL or more, and further preferably Suitable examples are body coolants capable of generating 200 million / mL or more, and more preferably 250 million / mL or more ultrafine bubbles in water. Further, the body cooling agent of the present invention, ultra-fine bubbles can be generated in the water (preferably, ultra-fine bubble CO ₂₎ as the upper limit of the concentration of, but not particularly limited, the following measurement methods R2 The concentration of ultrafine bubbles when measured in 1 is, for example, 10 billion / mL or less and 1 billion / mL or less. The body cooling agent of the present invention has a more specific concentration of ultra fine bubbles (preferably, CO ₂ ultra fine bubbles) that can be generated in water, from 5 million to 10 billion / mL, 5 million to 1 billion pieces / mL, 10 million to 10 billion pieces / mL, 10 million to 1 billion pieces / mL, 20 million to 10 billion pieces / mL, 20 million to 1 billion pieces / mL, 25 to 10 billion / mL, 25 to 1 billion / mL, 30 to 10 billion / mL, 30 to 1 billion / mL, 35 million to 10 billion pieces / mL, 35 million to 1 billion pieces / mL, 50 million to 10 billion pieces / mL, 50 million to 1 billion pieces / mL, 75 million to 10 billion pieces / mL, 75 million to 1 billion / mL, 100 to 10 billion / mL, 100 to 1 billion / mL, 150 to 10 billion / mL, 150 to 1 billion The number of particles / mL, 200 to 10 billion / mL, 200 to 1 billion / mL, 250 to 10 billion / mL, 250 to 1 billion / mL, and the like.
(Measurement method R2)
To 25 ° C. water, the body coolant -80～0 ° C., CO ₂ content in terms of 3% or more by weight of ice was added 300 mg / mL, ice CO ₂ content is 3 wt% or more After making ice water containing 0 to 2 ° C., the concentration (pieces / mL) of ultrafine bubbles in the ice water is measured with a Malvern Nanosite NS300.

(How to use body coolant)
The method of using the body cooling agent of the present invention will be described in detail in the section "Method for producing body cooling liquid of the present invention" described below, but the body cooling agent of the present invention is brought into contact with a liquid (preferably A preferable method is to prepare a body cooling liquid (containing it in a liquid) and apply the body cooling liquid to the skin (that is, contact with the skin). That is, the body cooling agent of the present invention is preferably for preparing a body cooling liquid for application to the skin at the time of use. The body cooling agent of the present invention can remarkably promote the blood flow in the skin when applied to the skin of an animal as the body cooling liquid. Those skilled in the art by reference to the present specification, the content and the CO ₂ high content of ice in the body coolant present invention (preferably CO ₂ hydrate), the CO ₂ high content of ice (preferably CO _The amount of the body coolant of the present invention can be adjusted according to the CO ₂ content of ( ₂ hydrate), the concentration of ultrafine bubbles required, and the like.

(liquid)
As the “liquid” in the present invention, when the high CO ₂ content ice (preferably CO ₂ hydrate) is contained in the liquid, the CO ₂ high content ice (preferably CO ₂ hydrate) is CO ₂ There is no particular limitation as long as it is a liquid that can generate bubbles (preferably ultrafine bubbles) and may be brought into contact with animal skin, and examples thereof include (i) "hydrophilic solvent" and (ii) " Examples thereof include a hydrophobic solvent, (iii) "a mixed solvent of a hydrophilic solvent and a hydrophobic solvent", "a liquid containing any solute in any of the solvents (i) to (iii)", and the like. The temperature condition and the pressure condition under which the "liquid" in the present invention is in a liquid state cannot be unconditionally specified because it depends on the kind of the solvent, the use of the liquid, the use condition of the liquid, etc. Preferable is a liquid which is in a liquid state under atmospheric pressure.

  The "hydrophilic solvent" used in the present invention preferably has a solubility parameter (SP value) of 20 or more, more preferably 29.9 or more. Specifically, it is preferable to use one or more selected from the group consisting of water (47.9), polyhydric alcohol and lower alcohol. Polyhydric alcohols such as ethylene glycol (29.9), diethylene glycol (24.8), triethylene glycol (21.9), tetraethylene glycol (20.3) and propylene glycol (25.8). , Triglycerides such as glycerin (33.8), diglycerin, triglycerin, polyglycerin, and trimethylolpropane; tetraglyceride alcohols such as diglycerin, triglycerin, polyglycerin, pentaerythritol, and sorbitol; and sorbitol. Examples thereof include aldoses such as hexitol and glucose, compounds having a sugar skeleton such as sucrose, and pentaerythritol. Examples of the lower alcohol include isopropanol (23.5), butyl alcohol (23.3) and ethyl alcohol (26.9). Two or more kinds of these hydrophilic solvents may be used in combination. In the parentheses, the δ value of the solubility parameter is shown. The preferred hydrophilic solvent in the present invention preferably contains at least water, and more preferably water.

  The "hydrophobic solvent" used in the present invention is preferably an organic solvent having a solubility parameter (SP value) of less than 20.0, specifically, a hydrocarbon solvent or a silicone solvent or those. Is a mixture of. Examples of hydrocarbon solvents include hexane (14.9), heptane (14.3), dodecane (16.2), cyclohexane (16.8), methylcyclohexane (16.1), octane (16.0). , Hydrogenated triisobutylene and other aliphatic hydrocarbons, benzene (18.8), toluene (18.2), ethylbenzene (18.0), xylene (18.0) and other aromatic hydrocarbons, chloroform (19. 3), 1,2-dichloroethane (19.9), trichloroethylene (19.1), and other halogen-based hydrocarbons, and the like. Examples of the silicone-based solvent include octamethylcyclotetrasiloxane and decamethylcyclopenta. Examples include siloxane, hexamethyldisiloxane, octamethyltrisiloxane and the like. Of these, hexane (14.9) and cyclohexane (16.8) are particularly preferable. Two or more kinds of these hydrophobic solvents may be used in combination.

As the “solute” in the above “liquid containing any solute in any of the solvents (i) to (iii)”, such liquid contains a high CO ₂ content ice (preferably CO ₂ hydrate). when brought into, CO ₂ high content of ice (preferably CO ₂ hydrate) of bubbles CO ₂ (preferably ultra fine bubbles) is not particularly limited as long as it can generate a. Specific examples of the “liquid containing any solute in the solvent of (i) to (iii)” include physiological saline.

(Arbitrary component)
The body cooling agent of the present invention contains CO ₂ -rich ice as an essential component, but as long as it does not interfere with the effect of the present invention (the effect of cooling the body while suppressing a decrease in blood flow), it is an optional component. May be further contained. Examples of such optional components include components having medicinal effects and additives. Examples of the component having such a medicinal effect include other body cooling agents, antibiotics, analgesics, antiphlogistics, etc., and the above-mentioned additives include fragrances, colorants, thickeners, pH adjusters and the like. .

(Applies to)
The animal to which the body cooling agent or body cooling liquid of the present invention is applied is not particularly limited, but an animal belonging to any group selected from the group consisting of mammals, birds, reptiles, amphibians and fish is preferable. Among them, among others, animals belonging to mammals or birds are more preferably listed, among them, animals belonging to mammals are more preferably listed, among others, humans, dogs, cats, horses, ponies, donkeys, cows, pigs, sheep, goats. , Rabbits, monkeys, mice, rats, hamsters, guinea pigs, ferrets and the like are more preferable, humans and horses are further preferable, and humans are particularly preferable. The horse is preferably a thoroughbred racehorse. This is because it is widely practiced to cool the limbs of the racehorse with ice water after the race. Cooling of racehorses is widely practiced because the racehorses have been improved so that they can run faster.As a result, they have slender limbs for their physique, and their legs break during races and training. There are many reasons why the racehorses tend to hurt and tendons are damaged, and races of modern racehorses are extremely fast, so that the racehorses after the race are very tired.

  The application of the body cooling agent or the body cooling liquid of the present invention is not particularly limited as long as the animal to which the application is applied requires a body cooling action. More specific uses of the body cooling agent of the present invention include, for example, a cooling agent for body icing before exercise, during exercise, and after exercise; for icing of body parts accompanied by acute inflammation such as bruise and acne. Cooling agents; cooling agents for cooling the body when generating heat; and the like.

(Applicable location)
Examples of the application site of the body cooling agent or the body cooling solution of the present invention include the whole or local skin of an animal. As used herein, the term "whole body" means the whole body within a range in which the animal can ensure breathing, and for example, in the case of mammals, the skin other than the head, that is, the skin below the neck is generally cooled by the body of the present invention. It means soaking in liquid. Further, in the present specification, the local part is not particularly limited as long as it is a part of the body, and examples thereof include a head, a face, a neck, shoulders, arms, hands, chest, abdomen, buttocks, legs and feet. The body coolant or body cooling fluid of the present invention may be applied to multiple parts of the body simultaneously. The “skin” in the present specification is not particularly limited as long as it is the skin of an animal, and also includes the mucous membrane of the animal for convenience. Examples of such mucous membranes include lips and mucous membranes in the oral cavity.

(Method of applying)
As a method of applying the body cooling agent of the present invention, the body cooling agent of the present invention may be directly applied to the skin of the application site, but a method of applying the body cooling liquid of the present invention to the skin of the application site is preferable. More specifically, a method of immersing the skin of the application site in the body cooling solution of the present invention, a method of applying the body cooling solution of the present invention to the skin of the application site, and the like are preferably mentioned. Among them, the method of immersing the skin at the application site in the body cooling liquid of the present invention is more preferable. The amount of use of CO ₂ -rich ice (preferably the amount added) (mg / mL) used when preparing the body cooling fluid of the present invention is as described in the item of the production method of the present invention.

(Temperature during use)
The temperature of the body cooling liquid when the body cooling liquid of the present invention is applied to the skin can be appropriately set according to the state of the body part to be cooled, the purpose of cooling, and the like, for example, 0 to 10 C, 0-8C, 0-6C, 0-4C, 0-3C, 0-2C, 2-10C, 2-8C, 2-6C, 2-4C, 4-10. C, 4-8 degreeC, 4-6 degreeC, etc. are mentioned. The method of adjusting the temperature of the body cooling liquid is not particularly limited, and for example, a method of adjusting the temperature of the liquid brought into contact with the body cooling agent or the temperature of the body cooling liquid is adjusted by a cooling device or a heating device. There is a method. As such a cooling device and a heating device, commercially available ones can be used.

The CO ₂ -rich ice (preferably CO ₂ hydrate) in the body cooling agent of the present invention is usually a solid, and when the CO ₂ -rich ice is brought into contact with a liquid to prepare a body cooling liquid, When a part of the CO ₂ -rich ice or it melts, it draws much heat from the liquid, so that the temperature of the liquid usually drops relatively large. Therefore, when the body cooling agent of the present invention is brought into contact with a liquid to prepare a body cooling liquid, it is preferable to use a liquid having a temperature higher than a desired temperature of the body cooling liquid. For example, it is preferable to use a liquid having a temperature higher by 2 ° C. or higher, 4 ° C. or higher, or 6 ° C. or higher than the desired temperature of the body cooling liquid to be prepared. In addition, the body cooling fluid of the present invention is preferably prepared before use when applied to the skin, from the viewpoint of obtaining a higher blood flow reduction suppressing effect (preferably a blood flow maintaining effect). In the present specification, “prepared before use” includes, for example, 1 from the time when the application of the body cooling liquid to the skin is started (the time when the contact of the body cooling liquid with the skin is started). Within the time, preferably within 40 minutes, more preferably within 30 minutes, even more preferably within 20 minutes, more preferably within 10 minutes, further preferably within 5 minutes, the body cooling of the present invention. Preparing a liquid is included.

(Applicable time)
The application time of the body cooling agent or the body cooling liquid of the present invention is not particularly limited as long as the effect of the present invention (the effect of cooling the body while suppressing the decrease in blood flow) can be obtained. It can be appropriately set according to the state of the part, the purpose of cooling, the temperature of the body cooling liquid, etc., but the body cooling liquid of the present invention is applied to the skin of the application site, for example, for 3 to 30 minutes, 5 to 25 minutes, 5 The contact may be performed for -20 minutes, 5-15 minutes, 10-20 minutes, 10-15 minutes.

(Application frequency)
The application frequency of the body cooling agent or the body cooling solution of the present invention is not particularly limited and may be appropriately determined based on the improvement of symptoms, for example, about 1 to 3 times in 1 to 3 days. Can be mentioned.

(Blood flow suppression effect)
In the present specification, the body cooling agent (or the CO ₂ -rich ice) of the present invention "has a blood flow lowering inhibitory effect" (or, the body cooling liquid of the present invention "has a blood flow lowering inhibitory effect". In that), the body cooling liquid (or the body cooling liquid of the present invention) obtained by contacting the liquid with the body cooling agent of the present invention (or ice with high CO ₂ content) is used in a room at 24 ° C. 5 minutes after the start of applying to the skin of the animal (preferably immersing the skin of the animal in the aforementioned body cooling liquid in a room at 24 ° C.) (preferably after 10 minutes) , And more preferably after 15 minutes have passed), the above-mentioned skin blood flow (hereinafter, also referred to as “the blood flow in the present invention”) has the same temperature as the above-mentioned body cooling liquid. In a room at ℃ 5 minutes after the start of applying the same to the skin at the same site (preferably immersing the animal's skin in the above-mentioned ice water or water in a room at 24 ° C., preferably 10 minutes) It is preferable that the blood flow rate in the present invention is higher than the above-mentioned skin blood flow rate (hereinafter, also referred to as “control blood flow rate”) at a time point, more preferably at a time point after 15 minutes have passed. It is preferably 5% or more, more preferably 10% or more, further preferably 15% or more, more preferably 20% or more more than the blood flow.

2. <Method for producing body cooling liquid of the present invention>
Examples of the method for producing a body cooling liquid of the present invention (production method of the present invention) include "ice having a CO ₂ content of 3% by weight or more (preferably CO ₂ hydrate)" (or "body cooling of the present invention. There is no particular limitation as long as it includes the step of bringing the agent ") into contact with a liquid. By CO ₂ high content of ice (preferably the CO ₂ hydrate) contacting the liquid, CO ₂ bubble (preferably ultra fine bubbles) can be produced body cooling liquid containing.

In the present specification, the "body cooling liquid" is not necessarily limited to the case where everything is in a liquid state, and includes the case where it is a mixture of solid CO ₂ -rich ice (preferably CO ₂ hydrate) and a liquid state. .

In the present specification, as a method of “contacting ice (preferably CO ₂ hydrate) having a CO ₂ content of 3% by weight or more with a liquid”, there are used a high CO ₂ content ice (preferably CO ₂ hydrate) and a liquid. not particularly limited as long as to make contact, CO ₂ high content of ice (preferably CO ₂ hydrate) method of incorporating into the liquid are preferably mentioned, among others, CO ₂ high content of ice (preferably CO ₂ hydrate) a a method of adding or put into a liquid, a method of CO ₂ high content of ice (preferably CO ₂ hydrate) is added liquid or put in. Among more preferred,, CO ₂ high content of ice (preferably CO ₂ Hyde More preferred is a method of adding or adding the rate) to the liquid.

The amount of CO ₂ high content of ice in the manufacturing method of the present invention (preferably amount) (mg / mL) is whether CO ₂ high content of ice is CO ₂ hydrate, at compaction CO ₂ hydrate whether, CO ₂ content of CO ₂ high content of ice, or (preferably ultra fine bubbles) what degree of the concentration of CO ₂ bubble depending on whether such require, those skilled in the art will appropriately be able to. The lower limit of the amount of use of CO ₂ -rich ice (preferably the amount added) (mg / mL) is, for example, 10 mg / mL or more, and CO ₂ bubbles (preferably ultrafine bubbles) are obtained at a higher concentration. From the viewpoint, it is preferably 20 mg / mL or more, more preferably 50 mg / mL or more, further preferably 100 mg / mL or more, more preferably 150 mg / mL or more, and further preferably 200 mg / mL or more. Further, the upper limit of the amount of use of CO ₂ -rich ice (preferably the amount of addition) (mg / mL) is not particularly limited, but for example, 5000 mg / mL or less, 3000 mg / mL or less, 2000 mg / mL or less, 1000 mg / mL Hereinafter, 500 mg / mL or less may be mentioned. As a more specific example of the usage amount (preferably added amount) (mg / mL) of CO ₂ -rich ice, 20-5000 mg / mL, 20-3000 mg / mL, 20-2000 mg / mL, 50- 2000 mg / mL, 50-1000 mg / mL, 100-500 mg / mL, 150-500 mg / mL are mentioned. The amount of CO ₂ -rich ice used (mg / mL) means the weight (mg) of CO ₂ -rich ice used (preferably added) per 1 mL of liquid.

The temperature of the liquid when the ice with high CO ₂ content is brought into contact with the liquid is not particularly limited as long as bubbles of CO ₂ (preferably ultra fine bubbles) are generated, and for example, 0 to 50 ° C., 0 to 35 ° C., 0. -25 ° C, 0-20 ° C, 0-15 ° C, 0-10 ° C, 0-7 ° C, 0-5 ° C, 3-50 ° C, 3-35 ° C, 3-25 ° C, 3-15 ° C, 3 -10 ° C, 3-7 ° C, 3-5 ° C, 6-50 ° C, 6-35 ° C, 6-25 ° C, 6-20 ° C, 6-15 ° C, 6-10 ° C, 6-7 ° C, 10 The temperature may be, for example, -50 ° C, 10-35 ° C, 10-25 ° C, 10-15 ° C, etc., but can be appropriately set by those skilled in the art depending on what temperature of the body-cooling liquid is desired. The CO ₂ -rich ice (preferably CO ₂ hydrate) in the body cooling agent of the present invention is usually solid, and when the CO ₂ -rich ice is brought into contact with a liquid to prepare a body cooling liquid, CO ₂ The temperature of the liquid will usually drop relatively significantly, as it will draw more heat from the liquid as part of or melts the high ice content. Therefore, when the body cooling agent of the present invention is brought into contact with a liquid to prepare a body cooling liquid, it is preferable to use a liquid having a temperature higher than a desired temperature of the body cooling liquid. For example, a liquid having a temperature higher by 2 ° C. or higher, 4 ° C. or higher or 6 ° C. or higher than the desired temperature of the body cooling liquid to be prepared, preferably 2-10 ° C., 4-10 ° C., 6-10 ° C. higher temperature. It is preferable to use a liquid.

3. <Body cooling fluid of the present invention>
The body cooling liquid of the present invention is a body cooling liquid for application to the skin. The body cooling fluid of the present invention is a liquid containing 200 ppm or more of carbonic acid, and has an ultrafine bubble concentration of 5 million cells / mL or more when measured by the above-mentioned measurement method R1 (or R2). It is not particularly limited as long as it is, but it is preferably a body cooling liquid produced by the production method of the present invention.

As described above, the “body cooling fluid” in the present specification is not limited to the case where all are liquid, and is a mixture of solid CO ₂ -rich ice (preferably CO ₂ hydrate) and liquid. Some cases are also included.

  The body cooling liquid of the present invention is not particularly limited as long as it contains 200 ppm or more of carbonic acid, but preferably 500 ppm (0.05% by weight) or more, more preferably 750 ppm (0.075% by weight) or more, further preferably It is preferable to contain carbonic acid in an amount of 900 ppm (0.09% by weight) or more, and more preferably 1000 ppm (0.1% by weight). The upper limit of carbonic acid is not particularly limited, but is, for example, 4000 ppm (0.4 wt%) or less, 3000 ppm (0.3 wt%) or less, 2000 ppm (0.2 wt%) or less, 1500 ppm (0.15 wt%) or less. Can be mentioned. More specifically as the carbonic acid concentration in the body cooling liquid of the present invention, more specifically, 500 to 4000 ppm, 750 to 4000 ppm, 900 to 4000 ppm, 1000 to 4000 ppm, 500 to 3000 ppm, 750 to 3000 ppm, 900 to 3000 ppm, 1000 to 3000 ppm, 500. .About.2000 ppm, 750 to 2000 ppm, 900 to 2000 ppm, 1000 to 2000 ppm, 500 to 1500 ppm, 750 to 1500 ppm, 900 to 1500 ppm, 1500 to 2000 ppm and the like.

  The carbonic acid concentration in the body cooling liquid of the present invention means the concentration measured at a liquid temperature of 0 to 2 ° C. and normal pressure.

The concentration of ultrafine bubbles (preferably, ultrafine bubbles of CO ₂ ) in the body cooling fluid of the present invention is 5 million / mL as the concentration (units / mL) when measured by the above-mentioned measurement method R1. It is not particularly limited as long as it is above, but is preferably 10 million pieces / mL or more, more preferably 20 million pieces / mL or more, further preferably 25 million pieces / mL or more, and more preferably 30 million pieces. / ML or more, more preferably 35 million pieces / mL or more, more preferably 50 million pieces / mL or more, further preferably 75 million pieces / mL or more, more preferably 100 million pieces / mL or more More preferably, it is 150 million pieces / mL or more, more preferably 200 million pieces / mL or more, and further preferably 250 million pieces / mL or more. The upper limit of the concentration of ultrafine bubbles (preferably, ultrafine bubbles of CO ₂ ) in the body cooling liquid of the present invention is not particularly limited, but ultrafine bubbles (when measured by the above-mentioned measuring method R1 ( Preferably, the concentration of CO ₂ ultra fine bubbles) is, for example, 10 billion cells / mL or less and 1 billion cells / mL or less. As a more specific concentration of ultrafine bubbles (preferably CO ₂ ultrafine bubbles) in the body cooling fluid of the present invention, the concentration (unit / mL) when measured by the above-described measurement method R1 (or R2). ), 5 million to 10 billion pieces / mL, 5 million to 1 billion pieces / mL, 10 million to 10 billion pieces / mL, 10 million to 1 billion pieces / mL, 20 million to 10 billion pieces / ML, 20 million to 1 billion pieces / mL, 25 million to 10 billion pieces / mL, 25 million to 1 billion pieces / mL, 30 million to 10 billion pieces / mL, 30 million 1 billion pieces / mL, 35 million to 10 billion pieces / mL, 35 million to 1 billion pieces / mL, 50 million to 10 billion pieces / mL, 50 million to 1 billion pieces / mL , 75 million to 10 billion pieces / mL, 75 million to 1 billion pieces / mL, 100 million to 10 billion pieces / mL, 100 million to 1 billion pieces / mL, 150 million Million to 10 billion / mL, 150 million to 1 billion / mL, 200 to 10 billion / mL, 200 to 1 billion / mL, 250 to 10 billion / mL, Examples include 250 million to 1 billion pieces / mL.

  The temperature of the body cooling liquid of the present invention can be appropriately set according to the state of the body part to be cooled, the purpose of cooling, etc., and is, for example, 0 to 10 ° C, 0 to 8 ° C, 0 to 6 ° C, 0. -4 ° C, 0-3 ° C, 0-2 ° C, 2-10 ° C, 2-8 ° C, 2-6 ° C, 2-4 ° C, 4-10 ° C, 4-8 ° C, 4-6 ° C, etc. Can be mentioned.

  The method for producing the body cooling fluid of the present invention is as described in the above “2.”.

  The body cooling liquid of the present invention may be contained in a container. The shape and material of the container are not particularly limited, and examples thereof include a plastic bottle container.

4. <Body cooling method of the present invention>
The body cooling method of the present invention is a method for cooling the body of an animal, preferably a method for cooling the body of an animal while suppressing a decrease in blood flow in the body of the animal. As the body cooling method of the present invention, CO ₂ -rich ice (preferably CO ₂ hydrate), the body cooling agent or the body cooling liquid of the present invention can be used for the whole or local skin of an animal (eg, non-human animal). There is no particular limitation as long as it includes a step applied to.

CO ₂ rich ice (preferably CO ₂ hydrate), the body cooling agent or the body cooling liquid of the present invention can be applied to (for example, contacted with) the whole or local skin of an animal by a high CO ₂ content. A method of bringing ice (preferably CO ₂ hydrate) or the blood flow promoting agent of the present invention into contact with the skin of the application site, a method of immersing the skin of the application site in the liquid for promoting blood flow of the present invention, the skin of the application site The preferred method is to apply the blood flow promoting liquid of the present invention, and among these, the method of immersing the skin at the application site in the blood flow promoting liquid of the present invention is more preferred.

5. <Other aspects of the present invention>
The present invention also includes the following aspects.
For cooling the body of an animal (preferably for cooling the body of an animal while suppressing a decrease in blood flow), ice containing high CO ₂ (preferably CO ₂ hydrate), body cooling of the present invention Use of agents or body coolants;
For cooling the body of an animal (preferably for cooling the body of an animal while suppressing a decrease in blood flow), ice containing high CO ₂ (preferably CO ₂ hydrate), body cooling of the present invention Method of using an agent or a body cooling liquid;
CO ₂ -rich ice (preferably CO ₂ hydrate) for use in the treatment of diseases that require a cooling effect on the body, a body cooling agent of the present invention or a body cooling fluid;
Use of ice with high CO ₂ content (preferably CO ₂ hydrate) in the manufacture of a body coolant or body cooling fluid according to the invention;

  Hereinafter, the present invention will be described in detail with reference to Examples, but the present invention is not limited to these Examples.

Test 1. [Preparation of CO ₂ hydrate]
Preparation of CO ₂ hydrate CO ₂ gas was blown into 4 L of water so as to have a pressure of 3 MPa, a CO ₂ hydrate formation reaction was allowed to proceed at 1 ° C. with stirring, and CO ₂ hydrate particles were suspended in water. “CO ₂ hydrate slurry” was obtained. The slurry was poured into a cylinder type compaction molding machine and compressed at a compression pressure of 2 MPa for 3 minutes. Then cooled to -20 ° C., it was recovered a cylindrical mass of compacted CO ₂ hydrate from the compacting machine to crush such cylindrical mass. A polyhedral compacted CO ₂ hydrate having a maximum length of 3 mm or more and 60 mm or less was selected and collected, and used in the subsequent experiments. The CO ₂ content of this consolidated CO ₂ hydrate was 24%, and the CO ₂ hydrate rate was about 85%.

Test 2. [Concentration and particle size of ultrafine bubbles in water containing CO ₂ hydrate]
Water to determine the concentration and particle size of the ultra-fine-bubble in CO ₂ hydrate containing ice water obtained by adding CO ₂ hydrate were compared with the concentration and the particle size of ultra-fine bubbles in the artificial carbonated ice or mere ice water.

(1) Preparation of Each Sample To 5 mL of water (about 26 ° C.), 1.5 g of CO ₂ hydrate prepared in Test 1 (about −80 ° C.) was added, and CO ₂ hydrate-containing ice water (0 to 0) was added. 2 ° C.) (carbonic acid concentration of 1000 ppm or more) was prepared.

  Carbon dioxide gas was added to 5 mL of ice water using an artificial carbonated spring manufacturing device (“Cleansui”, manufactured by Mitsubishi Chemical Corporation) and a carbon dioxide gas cylinder to prepare artificial carbonated ice water (0 to 2 ° C.) (carbon dioxide concentration of 1000 ppm or more).

  Simple ice water (0 to 2 ° C) was prepared.

(2) Measurement of Ultrafine Bubble Concentration and Particle Size For each of the CO ₂ hydrate-containing ice water, artificial carbonated ice water and mere ice water prepared in (1) above, bubbles were formed using Malvern's "Nanosite NS300". And the particle size were measured. FIG. 1 shows the particle size distribution of bubbles and the number concentration (number / mL) of ice water containing CO ₂ hydrate.

As shown in FIG. 1, it was confirmed that CO ₂ ultrafine bubbles were generated in water by adding CO ₂ hydrate to water. In the present CO ₂ hydrate-containing ice water (0 to 2 ° C.), ultrafine bubbles having a concentration of 550 million cells / mL were generated, and the median particle diameter was 125 nm. The concentration of ultra fine bubbles in mere ice water was 0.03.6 billion / mL, and the concentration of ultra fine bubbles in artificial carbonated ice water was 0.04.6 billion / mL. From these, it was shown that the concentration of ultrafine bubbles in the artificial carbonated ice water was only 1/100 or less of the concentration of the CO ₂ hydrate-containing ice water.

Test 3. [Confirmation of blood flow reduction suppressing effect by CO ₂ hydrate]
The following experiment was conducted in order to confirm what effect the CO ₂ hydrate-containing iced water has on blood flow of the skin when applied to the skin of an animal.

(1) Preparation of Each Sample Ice Water To 150 mL of water at 26 ° C., 45 g of CO ₂ hydrate (about -80 ° C.) prepared in Test 1 was added, and CO ₂ hydrate-containing ice water (0 to 2 ° C.) was added. (Carbonic acid concentration of 1000 ppm or more) was prepared.

  Carbon dioxide gas was applied to ice water obtained by adding 45 g of ice to 150 mL of water at 26 ° C. using an artificial carbonated spring producing device (“Cleansui”, manufactured by Mitsubishi Chemical Corporation) and a carbon dioxide gas cylinder, and artificial carbonated ice water (0 2 ° C.) (carbonic acid concentration of 1000 ppm or more) was prepared.

  45 g of ice was added to 150 mL of water at 26 ° C to prepare simple ice water (0 to 2 ° C).

(2) Blood flow measurement test For the blood flow measurement test, a Wistar male with a body weight of about 300 g, which was bred for 1 week in a constant temperature animal room at 24 ° C. under a 12-hour light / dark cycle (lighted from 8:00 to 20:00). Rats were used. For blood flow measurement, a probe (diameter 1 cm) of a laser blood flow meter (ALF21, manufactured by Advance Co., Ltd.) was fixed with a surgical tape to a portion near the origin of the dorsal surface of the rat tail, and the blood flow rate (mL / Min / 100 g tissue) was measured.

  When the blood flow data of the rat was stable, the blood flow of the skin of the tail was measured while immersing the rat tail in each sample ice water prepared in (1) above for 30 minutes. Then, each sample ice water was removed from the tail, and the blood flow in the skin of the tail was measured for 30 minutes. When the probe of the blood flow meter comes into contact with the sample ice water, the measured value shifts. Therefore, the tail was immersed in the sample ice water so that the probe was located slightly above the immersion part of the tail. Further, during the blood flow measurement test, the body temperature (rat rectal temperature) was kept at 37.0 ± 0.5 ° C. with a heat retaining device. Blood flow data was collected using a Power-Lab analog-to-digital converter. For blood flow data, the average value of blood flow every 5 minutes (ml / min / 100 g of tissue) was adopted, and the average value (0 min value) for 5 minutes before the start of tail immersion in sample ice water was defined as 100%. Expressed as It was confirmed by a thermometer that the temperature of each sample ice water for 30 minutes in which the tail was immersed in each sample ice water was maintained at 0 to 2 ° C. The results of this blood flow measurement test are shown in FIG.

  As shown in FIG. 2, when the tail is immersed in mere ice water (“ice water”), the blood flow in the skin of the tail gradually decreases, reaching a minimum value of 66.0% 30 minutes after the start of immersion. The blood flow rate remained at a value around 60 minutes after the start of immersion (that is, 30 minutes after the completion of immersion), and became 67.1% 30 minutes after the completion of immersion.

  On the other hand, when the tail is immersed in artificial carbonated ice water, the blood flow in the skin of the tail gradually decreases, reaching 80.0% 15 minutes after the start of immersion, and after that, it stays at a value near that point and starts immersion. Although the minimum value became 75.7% after 45 minutes (that is, 15 minutes from the end of immersion), it slightly increased and 60 minutes after the start of immersion (that is, 30 minutes after the completion of immersion). ) Was 88.5%.

On the other hand, when the tail is immersed in the CO ₂ hydrate-containing ice water, the blood flow rate of the skin of the tail is slightly increased as a whole, and even after the CO ₂ hydrate-containing ice water is removed from the tail, the blood flow rate before the start of immersion ( 100%). The maximum value of blood flow (107.4%) was measured 60 minutes after the start of immersion (that is, 30 minutes after the completion of immersion).

These results, the low temperature liquid containing CO ₂ hydrate (e.g. CO ₂ hydrate containing ice water), when applied to the skin of an animal's body, while suppressing a decrease in blood flow that skin cooling body It has been proven that it can be done. It has been proved that such an effect cannot be achieved even with mere low temperature water (for example, mere ice water) and cannot be achieved even with low temperature artificial carbonated springs (for example, artificial carbonated ice water). From these, when the body is iced with a low-temperature liquid containing ice (CO ₂ hydrate) having a CO ₂ content of 3% by weight or more, a decrease in blood flow, which has been a problem in the conventional icing method, is reduced. It is thought that as a result, it is possible to obtain the effect of suppressing inflammation by cooling, the effect of removing fatigue substances, and the effect of maintaining exercise performance.

  According to the present invention, there is provided a body cooling agent capable of cooling the body while suppressing a decrease in blood flow, a method for producing a body cooling solution including a step of bringing the body cooling agent into contact with a liquid, and the like. be able to.

Claims (8)

A body cooling agent comprising ice having a CO ₂ content of 3% by weight or more. The body cooling agent according to claim 1, wherein the ice having a CO ₂ content of 3% by weight or more is a CO ₂ hydrate. The body cooling agent according to claim 1 or 2, wherein the ice having a CO ₂ content of 3% by weight or more is an ice having a maximum length of 3 mm or more and a CO ₂ content of 3% by weight or more. Generation of ultra fine bubbles in water such that ice having a CO ₂ content of 3% by weight or more has an ultra fine bubble concentration of 5 million cells / mL or more when measured by the following measuring method R1. Body cooling agent according to any of claims 1 to 3, characterized in that it is ice that can be applied.
(Measurement method R1)
To water at 25 ° C, 300 mg / mL of ice having a CO ₂ content of 3% by weight or more and -80 to 0 ° C is added to contain ice having a CO ₂ content of 3% by weight or more. After making ice water at 0 to 2 ° C., the concentration of ultrafine bubbles (in pieces / mL) in the ice water is measured by Malvern Nanosite NS300. The body coolant according to any one of claims 1 to 4, wherein the ice having a CO ₂ content of 3% by weight or more is a consolidated CO ₂ hydrate.   The body cooling agent according to any one of claims 1 to 5, which is for preparing a body cooling solution for application to the skin at the time of use.   A method for producing a body cooling liquid for application to the skin, comprising the step of bringing the body cooling agent according to any one of claims 1 to 6 into contact with a liquid. A body cooling liquid for application to the skin, which is a liquid containing 200 ppm or more of carbonic acid, and has an ultrafine bubble concentration of 5 million cells / mL or more when measured by the following measuring method R1.
(Measurement method R1)
To water at 25 ° C, 300 mg / mL of ice having a CO ₂ content of 3% by weight or more and -80 to 0 ° C is added to contain ice having a CO ₂ content of 3% by weight or more. After making ice water at 0 to 2 ° C., the concentration of ultrafine bubbles (in pieces / mL) in the ice water is measured by Malvern Nanosite NS300.