JP2020030230A - Microscope observation heating device - Google Patents

Abstract

To provide a microscope observation heating device that can surely fix slide glass, and makes attaching/detaching work of the slide glass easy.SOLUTION: When pulling one long-side rim 73A of slide glass 73 in a pressing direction with respect to a guide surface 16, the one long-side rim 73A abuts on an abutting surface 26 of an abutting part 25, and the abutting surface 26 is evacuated at an evacuation space 21 side by elastic revolution with a base end 23A of an arm 23 as a center, and a protrusion position retreats to the guide surface 16. In this state, when a hand is released from the slide glass 73 with the other long-side rim 73B of the slide glass 73 lowered, and fitted into a guide surface 18 side, the slide glass 73 is loaded on a heating load surface 61 in a state with the slide glass elastically pressed between the guide surfaces 16 and 18, and a position of the slide glass is fixed. Left and right sides of the heating load surface 61 are kept open, and thus, only by causing the slide glass 73 to oscillate, the slide glass 73 is caused to get through from the loading part 3, and can be detached.SELECTED DRAWING: Figure 7

Description

  The present invention relates to a heating apparatus for microscopic observation, and more particularly to a heating apparatus for microscopic observation that can observe a specimen such as sperm placed on a slide glass while heating.

  A slide glass is widely used for mounting a sample when performing microscope observation. However, when a microscope requiring heating such as sperm is to be observed with a microscope, for example, a slide glass is disclosed in Patent Document 1. 2. The number of spermatozoa is counted and the motility is observed while the sample is placed on the heating mounting surface of the heating device for microscopic observation shown in 2 and heated. Then, the heating mounting surface is moved in the horizontal direction by the driving device as needed.

JP-A-7-92063 JP-A-11-38329

In the above-mentioned conventional heating apparatus for microscopic observation, the slide glass is merely mounted on the heating mounting surface and is not fixed. Therefore, if the heated mounting surface is moved or touches the slide glass carelessly, the slide glass may move relatively from a reference position positioned with respect to the heated mounting surface. .
If the slide moves from the reference position, the observation must be repeated.
On the other hand, such observation is often performed by using one microscope for many specimens, and it is necessary to sequentially attach and detach a slide glass to and from the heated mounting surface. For this reason, if the slide glass is easily fixed, the attaching / detaching operation is troublesome.

  SUMMARY OF THE INVENTION The present invention has been made in view of the above-mentioned conventional problems, and provides a heating apparatus for microscope observation that can securely fix a slide glass and that can easily perform a work of attaching and detaching the slide glass. Is its purpose.

  SUMMARY OF THE INVENTION The present invention has been made to solve the above-mentioned problem, and the invention of claim 1 has a mounting portion having an upper surface as a heated mounting surface of a slide glass, and an upper portion sandwiching the heated mounting surface. A pair of guide surfaces provided to protrude, a retreat space provided by retreating one guide surface, and projecting from the one guide surface toward the other guide surface to elastically contact the contact partner. While being pressed, the slide glass is provided with a pressing piece that resiliently rotates and retracts in the retreat space by the amount of the rotation, and the slide glass becomes a contact partner when the slide glass is placed on the heating mounting surface. The presser piece is pressed toward the other guide surface to fix the position, but at the same time, it is also allowed to pass through by sliding between the pair of guide surfaces. is there.

  According to a second aspect of the present invention, in the heating apparatus for microscopic observation according to the first aspect, the holding piece includes an arm that rotates with one end side of the guide surface side of the evacuation space as a base end, and a play of the arm. A heating device for microscopic observation, comprising a contact portion bulging toward the other guide surface on the end side.

  According to a third aspect of the present invention, in the heating apparatus for microscopic observation according to the second aspect, the presser piece and the retreat space are formed by using an outline of an edge of the upper protruding portion of the mounting portion on the guide surface side. A heating device for microscopic observation.

  According to a fourth aspect of the present invention, in the heating apparatus for microscopic observation according to any one of the first to third aspects, the heating mounting surface has a rectangular shape, and a pair of guide surfaces is provided on two opposing sides. The heating apparatus for microscopic observation is characterized in that the two opposite sides of the stand are opened, and the slide glass can pass through the slide glass in the mounted posture.

  According to a fifth aspect of the present invention, in the heating device for microscopic observation according to any one of the first to fourth aspects, the heating device is provided on the moving device side so as to be mounted on a microscope stage and mounted on a moving device that moves in a horizontal direction. A pair of fixing concave portions fixed to the pair of fixing convex portions by concave-convex fitting are provided on the mounting portion, which is a heating device for microscope observation.

  ADVANTAGE OF THE INVENTION According to the heating device for microscope observation of this invention, a slide glass can be fixed reliably, and also it becomes possible to perform the attachment / detachment operation of a slide glass easily. Therefore, it is possible to reduce the burden on the worker and to improve the working efficiency.

FIG. 1 is a perspective view of a heating device for microscopic observation according to an embodiment of the present invention in a state where an upper surface is directed upward. FIG. 1 is a perspective view of a heating device for microscopic observation according to an embodiment of the present invention in a state where a lower surface is directed upward. FIG. 2 is an exploded perspective view of the heating device for microscopic observation in FIG. 1. (A) is a top view of the heating device for microscopic observation of FIG. 1, (b) is a right side view. 4A is a sectional view taken along line AA of FIG. 4A, FIG. 4B is a sectional view taken along line BB of FIG. 4A, and FIG. 4C is a sectional view taken along line CC of FIG. FIG. 2 is a perspective view showing a state in which the heating device for microscope observation in FIG. 1 is mounted on a moving device installed on a stage of a microscope. FIG. 2 is a perspective view for explaining an operation of placing a slide glass on the heating device for microscopic observation in FIG. 1. FIG. 2 is a perspective view showing a state where a slide glass is placed on the heating device for microscopic observation in FIG. 1. (A) is a top view in the state where the slide glass was mounted on the heating device for microscopic observation of FIG. 1, and (b) is a right side view. FIG. 2 is a perspective view for explaining an operation of removing a slide glass placed on the heating device for microscopic observation in FIG. 1. FIG. 2 is a perspective view of a state where the heating device for microscope observation of FIG. 1 is mounted on another type of moving device installed on a stage of a microscope.

A heating device for microscope observation 1 according to an embodiment of the present invention will be described with reference to the drawings.
Hereinafter, the direction indicated by the arrow in FIG. 1 will be described as a reference for the up, down, front, rear, left and right directions of the heating device 1 for microscopic observation.
As shown in FIGS. 1 and 2, reference numeral 3 denotes a mounting portion, and the mounting portion 3 is configured by combining a frame-shaped main body 5 and a back cover 33. The mounting portion 3 is made of ABS resin and has elasticity.

As shown in FIG. 3, the frame-shaped main body 5 has a rectangular cross-section in the vertical direction, with the long side extending in the left-right direction and the short side extending in the front-rear direction. In the vertical direction, it has a thickness that can accommodate a transparent heating plate of a heater described later.
At the center of the upper surface of the frame-shaped main body 5, a large rectangular opening 7 is formed in a similar manner. A step 9 is provided at an intermediate portion on the inner wall of the pair of long sides facing the opening 7, and a step below the step 9 is widened by the step difference (see FIG. 5).
The pair of short sides of the upper surface of the frame-shaped main body 5 have a pair of cooperating portions 11 and 13 with the upper surface lowered by one step except for the end portions in the front-rear direction. These upper surfaces are horizontal and at the same height. The upper surface position of the linking units 11 and 13 is lower by about 1.0 mm than the other upper surface positions.

  Due to the presence of the link portions 11 and 13, the upper surfaces of the remaining pair of long sides protrude upward one step higher. The pair of long sides extend in a flange shape toward the facing directions, and the edges of the flange portions 15 and 17 are the edges of the opening 7 in the front-rear direction. This edge is a vertical surface, which forms the guide surfaces 16,18.

  As shown in the enlarged view of FIG. 3, a notch 19 is provided in the rear flange portion 15. The notch 19 has an L-shape when viewed from above, and extends while retracting rearward with the guide surface 16 as a starting end, changes its extending direction, and passes rightward through a substantially right-angled corner. It extends to the terminal end 19A, and has a certain width dimension in a direction orthogonal to the extending direction. The width dimension of the right extending notch portion 19C is smaller than the width dimension of the rearward extending notch portion 19B. The two corners on the way and the terminal end 19A are rounded. The evacuation space 21 is constituted by the planar notch 19.

At the front side of the cutout portion 19C extending to the right, there is a portion left without being cut out, and this portion constitutes the arm 23. The base end 23A of the arm 23 is a portion adjacent to the front end 19A on the front side.
The width dimension of the arm 23 crossing in the front-rear direction is substantially the same, but the free end portion at the tip is bulged forward and forms a square shape. The contact portion 25 is formed by the square portion. The contact portion 25 is configured such that another rectangular portion is connected to the front side of the rectangular portion connected to the arm 23, and the contact portion 25 projects from the guide surface 16 toward the front guide surface 18 by the amount of the connected portion. I have. The edge of the contact portion 25 is a vertical surface like the guide surface 16 and is continuous with the guide surface 16. A contact surface 26 is formed of a portion of the end edge of the contact portion 25 which is parallel to the guide surface 16 in particular.
The position of the notch 19 is adjusted so that the contact portion 25 is located substantially at the center of the rear flange portion 15 in the left-right direction.
As described above, the evacuation space 21, the arm 23, and the contact portion 25 are configured by the outline of the edge of the rear flange portion 15.

Fixing recesses 27, 27 are respectively formed on the outer surfaces (left and right side surfaces of the frame-shaped main body 5) of the cooperation portions 11, 13 on both left and right sides of the frame-shaped main body 5. The fixing recesses 27 are located closer to a diagonal line of the frame-shaped main body 5. Further, the fixing recess 27 is recessed inward and is opened not only on the side but also below. The inner surfaces of the cooperating portions 11 and 13 are bulged by the recesses of the fixing concave portions 27 and 27.
A notch 31 for inserting the electric cord 29 is formed on the front end surface of the frame-shaped main body 5.
The corners that appear outside the frame-shaped main body 5 are chamfered and formed in a round shape.

A back cover 33 is provided to protect the inside of the frame-shaped main body 5 by contacting the frame-shaped main body 5 configured as described above from behind.
The back cover 33 has a flat plate shape, and an opening 35 is formed in a similar shape. A notch 37 is formed in the opening 35. The opening 35 and the notch 37 have contours corresponding to the opening 7 and the notch 19 of the frame-shaped main body 5.
Notches 39, 39 are formed on the left and right end surfaces of the back cover 33 so as to correspond to bulging portions of the fixing recesses 27, 27 of the frame-shaped main body 5 toward the inner side. Further, a projecting piece 41 is formed on the front end face of the back cover 33, and the projecting piece 41 corresponds to the notch 31 of the frame-shaped main body 5.

The heated mounting surface of the slide glass in the frame-shaped main body 5 is configured by the upper surface of the glass heater 43.
The glass heater 43 has a rectangular plate shape. The glass heater 43 includes a lower glass 45 having a transparent conductive film attached to an upper surface thereof, a pair of electrodes provided in contact with the upper glass 47 and the transparent conductive film of the lower glass 45 which are stacked and fixed on the lower glass 45 from above. 49 and 51. Lead wires (not shown) are connected to the electrodes 49 and 51.
A temperature sensor 53 is fixed to the lower surface of the glass heater 43, and the temperature sensor 53 is covered with a protective cover 55 so as not to be exposed on the back surface. A lead wire (not shown) is also connected to the temperature sensor 53. The lead wire is a strip of the electric cord 29 inserted from the notch 31 together with the lead wires connected to the electrodes 49 and 51. The electric cord 29 is connected to a controller (not shown).

  The transparent conductive film of the glass heater 43 is energized and heated through the electric cord 29 and the pair of electrodes 49 and 51. The value detected by the temperature sensor 53 is transmitted to the controller, and the energization of the transparent conductive film is controlled based on the detection result, so that the temperature is maintained at the set temperature.

As shown in FIGS. 2, 3, and 5, the glass heater 43 is housed in the frame-shaped main body 5 from the lower surface side and is provided so as to close the opening 7. The lower surfaces of the flange portions 15 and 17 are fixed by double-sided adhesive tapes 57 and 59. In this state, the glass heater 43 is exposed from the opening 7 of the frame-shaped main body 5 except for the edge, and the exposed upper surface serves as a heated mounting surface 61 (see FIG. 1) of the slide glass. The heating mounting surface 61 is at the same height as the upper surfaces of the cooperation units 11 and 13. The pair of flange portions 15 and 17 project 1.0 mm above the heating mounting surface 61.
In this fixed state, as shown in FIG. 4A, the arm 23 is in a state of playing from its base end 23A, and as shown by the phantom line, the upper surface of the glass heater 43 is centered on the base end 23A. The elastic rotation while sliding is enabled. Therefore, the abutment surface 26 can be retracted into the evacuation space 21 until the protruding end thereof is substantially flush with the guide surface 16.
The back cover 33 is housed in the frame-shaped main body 5 from the lower surface side and is fixed in a state of contacting the step 9 (see FIG. 5).
Since the heating device for microscope observation 1 is configured as described above, the heating mounting surface 61 is opened flush with the left and right directions.

Next, a method of using the culture device 1 for microscopic observation will be described.
In FIG. 6, reference numeral 63 denotes a microscope stage, on which a base 67 of a moving device 65 is fixed.
The mounting portion 3 of the culture device 1 for microscopic observation is mounted on the base 67. The right side surface is in contact with the edge of the fixed contact portion 69, and the left side surface is pressed rightward by the pressing portion 71 and is fixed in a pressed state. The pressing portion 71 is urged rightward by a spring (not shown), and the mounting portion 3 is pressed against the urging member by using this urging. The pressing portion 71 is provided with an insertion portion, and the mounting portion 3 is mounted. When the pressing portion 71 moves in the urging direction, the pressing portion 71 enters the fixing concave portion 27 and is fixed by concave and convex fitting.

The operation of placing the slide glass 73 on the culture device 1 for microscopic observation will be described.
As shown in FIG. 7, when one long side edge 73 </ b> A of the slide glass 73 is moved obliquely downward in the direction of pressing against the guide surface 16, the slide glass 73 first comes into contact with the contact surface 26 of the contact portion 25. The contact surface 26 retreats toward the retreat space 21 by elastic rotation about the base end 23A of the arm 23, and the projecting position retreats to the guide surface 16.
By this retreat, the long side edge 73A of the slide glass 73 comes into contact with the (guide surface 16 to contact surface 26 to guide surface 16). In this state, the other long side edge 73B of the slide glass 73 is lowered and put into the guide surface 18 side, and when the slide glass 73 is released, as shown in FIGS. It is placed on the heating placement surface 61 in a state where it is elastically pressed between 16 and 18, and the position is fixed as shown in FIG. Thus, the slide glass 73 can be easily installed with one hand.

The thickness of the slide glass 73 is 1.0 mm, and the upper surface of the slide glass 73 and the upper surfaces of the flange portions 15 and 17 connected to the guide surfaces 16 and 18 have the same height.
The slide glass 73 is heated by receiving heat from the glass heater 43.

Then, by operating the handles 75 and 77 of the moving device 65, the slide glass 73 is moved together with the mounting portion 3 of the culture device 1 for microscopic observation, and the observation target portion, for example, sperm on the slide glass 73 is moved to the objective lens 79. The number of spermatozoa on the slide glass 73 is counted, and the motility is observed.
Since the slide glass 73 is guided in a press-contact state on the guide surfaces 16 and 18 and is fixed in a state where the slide glass 73 is mounted on the heating mounting surface 61, it is possible to prevent the slide glass 73 from inadvertently moving during observation. Can be prevented. Therefore, the slide glass 73 does not deviate from the objective lens 79, and the inconvenience of having to recount the number can be avoided.
Further, as described above, since the upper surface of the slide glass 73 and the upper surfaces of the flange portions 15 and 17 have the same height, even when the objective lens 79 is brought close to the upper surface of the slide glass 3 particularly when performing observation at a high magnification. Inconvenience that the objective lens 79 contacts the upper surfaces of the flange portions 15 and 17 does not occur.

When the observation on the slide glass 73 mounted on the culture device for microscopic observation 1 is completed, the slide glass 73 is slid in either the left or right direction of the heated mounting surface 61. Since the left and right sides of the heating placement surface 61 are open, the slide glass 73 can be passed through the placement section 3 and removed by simply sliding the slide glass 73. Since the slide glass 73 does not need to be tilted, sperm thereon does not slide down.
Then, the slide glass 73 to be newly observed is set as described above.
As described above, in the culture device 1 for microscopic observation, the slide glasses 73 can be easily attached and detached, and even when a large number of slide glasses 73 are successively attached and detached, the burden on the operator can be reduced and the operation efficiency can be improved. It is possible.

FIG. 11 shows a state in which the culture device for microscope observation 1 is mounted on a moving device 85 of a type different from the moving device 65.
The pressing portion 87 of the moving device 85 is fixed to the corner portion of the frame-shaped main body 5 of the heating device 1 for microscopic observation by pressing it against the corner portion. Even in this case, since the corners of the frame-shaped main body 5 are chamfered and formed in a round shape, the heating device for microscope observation 1 is securely fixed to the moving device 85.

Although the embodiment of the present invention has been described in detail above, the specific configuration is not limited to this embodiment, and even if there is a change in the design without departing from the gist of the present invention, the invention is not limited to the embodiment. included.
For example, in the above-described embodiment, the heating mounting surface is configured to be open in the left-right direction, but may be configured to open any one side. In consideration of the thickness of commercially available slide glasses, the height of the guide surfaces 16 and 18 from the heating mounting surface 61 is preferably set in a range of 0.5 mm to 2.0 mm.
Further, the frame-shaped main body 5 of the mounting portion 3 is not particularly limited to the ABS resin, but may be other plastic or metal as long as the elastic function expected in the present invention can be exhibited.

DESCRIPTION OF SYMBOLS 1 ... Heating apparatus for microscope observation 3 ... Mounting part 5 ... Frame-shaped main body 7 ... Opening 9 ... Step 11, 13 ... Cooperation part 15, 17 ... Flange part 16, 18 ... Guide surface 19 ... Notch 19A ... Terminal 19B: rearward extending notch portion 19C ... rightward extending notch portion 21 ... evacuation space 23 ... arm 25 ... contact portion 26 ... contact surface 27 ... fixing concave portion 29 ... electric cord 31 ... notch 33 ... back cover 35 ... Opening 37 ... Notch 39 ... Notch 41 ... Projection piece 43 ... Glass heater 45 ... Lower glass 47 ... Upper glass 49, 51 ... Electrode 53 ... Temperature sensor 55 ... Protective cover 57, 59 ... Double-sided adhesive tape 61 ... Addition Warm mounting surface 63 ... Stage 65 ... Moving device 67 ... Base 69 ... Fixed contact portion 71 ... Pressing portion 73 ... Slide glass 75, 77 ... Handle 79 ... Objective lens 85 ... Moving device 87 ... Pressing part

Claims (5)

A mounting portion having an upper surface as a heated mounting surface of the slide glass, a pair of guide surfaces protruding upward with the heated mounting surface interposed therebetween, and one of the guide surfaces is provided to be retracted. A retreat space, protruding from the one guide surface toward the other guide surface, elastically presses a contact partner, and revolves elastically to retreat to the retreat space by the amount of rotation. With a holding piece,
When the slide glass is placed on the heated mounting surface, the slide glass is pressed against the other guide surface by the pressing piece as a contact partner and is fixed in position, but at the same time, between the pair of guide surfaces. A heating device for microscopic observation, characterized in that it is also allowed to pass through by sliding. The heating device for microscopic observation according to claim 1,
The holding piece is composed of an arm that rotates with one end side on the guide surface side of the evacuation space as a base end, and a contact portion that bulges toward the other guide surface on the free end side of the arm. A heating device for microscopic observation. The heating device for microscopic observation according to claim 2,
A heating device for microscope observation, wherein the presser piece and the retreat space are formed by using the contour of the edge of the mounting portion on the guide surface side of the upwardly projecting portion. The heating device for microscopic observation according to any one of claims 1 to 3,
The heating mounting surface has a rectangular shape, and a pair of guide surfaces rise on two opposing sides, and the remaining two opposing sides are open, so that the slide glass can pass through in the mounting posture. A heating device for microscopic observation. The heating device for microscopic observation according to any one of claims 1 to 4,
A pair of fixing recesses, which are fixed to the pair of fixing protrusions provided on the moving device side by uneven fitting so as to be mounted on a moving device that is mounted on the microscope stage and moves in the horizontal direction, has a mounting portion. A heating device for microscopic observation, wherein the heating device is provided for a microscope.

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