JP2020022440A5 - - Google Patents

Info

Publication number

JP2020022440A5

JP2020022440A5 JP2019135853A JP2019135853A JP2020022440A5 JP 2020022440 A5 JP2020022440 A5 JP 2020022440A5 JP 2019135853 A JP2019135853 A JP 2019135853A JP 2019135853 A JP2019135853 A JP 2019135853A JP 2020022440 A5 JP2020022440 A5 JP 2020022440A5

Authority

JP

Japan

Prior art keywords

endo

sugar chain

complex

type sugar

amino acid

Prior art date

2019-07-24

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
• Global Dossier
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• 235000000346 sugar Nutrition 0.000 claims description 54
• 102000004169 proteins and genes Human genes 0.000 claims description 21
• 108090000623 proteins and genes Proteins 0.000 claims description 21
• 238000000034 method Methods 0.000 claims description 13
• 102000004190 Enzymes Human genes 0.000 claims description 10
• 108090000790 Enzymes Proteins 0.000 claims description 10
• 108090000288 Glycoproteins Proteins 0.000 claims description 8
• 102000003886 Glycoproteins Human genes 0.000 claims description 8
• 102000002068 Glycopeptides Human genes 0.000 claims description 7
• 108010015899 Glycopeptides Proteins 0.000 claims description 7
• OVRNDRQMDRJTHS-RTRLPJTCSA-N N-acetyl-D-glucosamine Chemical group CC(=O)N[C@H]1C(O)O[C@H](CO)[C@@H](O)[C@@H]1O OVRNDRQMDRJTHS-RTRLPJTCSA-N 0.000 claims description 6
• 230000000694 effects Effects 0.000 claims description 4
• 125000003275 alpha amino acid group Chemical group 0.000 claims 12
• 102000005744 Glycoside Hydrolases Human genes 0.000 claims 8
• 108010031186 Glycoside Hydrolases Proteins 0.000 claims 8
• 102100035149 Cytosolic endo-beta-N-acetylglucosaminidase Human genes 0.000 claims 7
• 101710144190 Endo-beta-N-acetylglucosaminidase Proteins 0.000 claims 7
• 230000002255 enzymatic effect Effects 0.000 claims 6
• 241000894006 Bacteria Species 0.000 claims 5
• DQJCDTNMLBYVAY-ZXXIYAEKSA-N (2S,5R,10R,13R)-16-{[(2R,3S,4R,5R)-3-{[(2S,3R,4R,5S,6R)-3-acetamido-4,5-dihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy}-5-(ethylamino)-6-hydroxy-2-(hydroxymethyl)oxan-4-yl]oxy}-5-(4-aminobutyl)-10-carbamoyl-2,13-dimethyl-4,7,12,15-tetraoxo-3,6,11,14-tetraazaheptadecan-1-oic acid Chemical compound NCCCC[C@H](C(=O)N[C@@H](C)C(O)=O)NC(=O)CC[C@H](C(N)=O)NC(=O)[C@@H](C)NC(=O)C(C)O[C@@H]1[C@@H](NCC)C(O)O[C@H](CO)[C@H]1O[C@H]1[C@H](NC(C)=O)[C@@H](O)[C@H](O)[C@@H](CO)O1 DQJCDTNMLBYVAY-ZXXIYAEKSA-N 0.000 claims 3
• 241001470488 Tannerella Species 0.000 claims 3
• 241000606125 Bacteroides Species 0.000 claims 2
• 241001015906 Muribaculum Species 0.000 claims 2
• 238000004519 manufacturing process Methods 0.000 claims 2
• 244000005700 microbiome Species 0.000 claims 2
• 150000008163 sugars Chemical class 0.000 claims 2
• MSWZFWKMSRAUBD-IVMDWMLBSA-N 2-amino-2-deoxy-D-glucopyranose Chemical compound N[C@H]1C(O)O[C@H](CO)[C@@H](O)[C@@H]1O MSWZFWKMSRAUBD-IVMDWMLBSA-N 0.000 claims 1
• 125000003047 N-acetyl group Chemical group 0.000 claims 1
• WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 claims 1
• MSWZFWKMSRAUBD-UHFFFAOYSA-N beta-D-galactosamine Natural products NC1C(O)OC(CO)C(O)C1O MSWZFWKMSRAUBD-UHFFFAOYSA-N 0.000 claims 1
• SQVRNKJHWKZAKO-UHFFFAOYSA-N beta-N-Acetyl-D-neuraminic acid Natural products CC(=O)NC1C(O)CC(O)(C(O)=O)OC1C(O)C(O)CO SQVRNKJHWKZAKO-UHFFFAOYSA-N 0.000 claims 1
• 235000021310 complex sugar Nutrition 0.000 claims 1
• 229930182830 galactose Natural products 0.000 claims 1
• 229960002442 glucosamine Drugs 0.000 claims 1
• SQVRNKJHWKZAKO-OQPLDHBCSA-N sialic acid Chemical compound CC(=O)N[C@@H]1[C@@H](O)C[C@@](O)(C(O)=O)OC1[C@H](O)[C@H](O)CO SQVRNKJHWKZAKO-OQPLDHBCSA-N 0.000 claims 1
• 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 7
• OVRNDRQMDRJTHS-UHFFFAOYSA-N N-acelyl-D-glucosamine Natural products CC(=O)NC1C(O)OC(CO)C(O)C1O OVRNDRQMDRJTHS-UHFFFAOYSA-N 0.000 description 6
• MBLBDJOUHNCFQT-LXGUWJNJSA-N N-acetylglucosamine Natural products CC(=O)N[C@@H](C=O)[C@@H](O)[C@H](O)[C@H](O)CO MBLBDJOUHNCFQT-LXGUWJNJSA-N 0.000 description 6
• 108010064983 Ovomucin Proteins 0.000 description 6
• 239000007795 chemical reaction product Substances 0.000 description 6
• 108010066476 ribonuclease B Proteins 0.000 description 6
• 238000003776 cleavage reaction Methods 0.000 description 5
• 239000000758 substrate Substances 0.000 description 5
• 238000006206 glycosylation reaction Methods 0.000 description 4
• 238000004949 mass spectrometry Methods 0.000 description 4
• OVRNDRQMDRJTHS-FMDGEEDCSA-N N-acetyl-beta-D-glucosamine Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O OVRNDRQMDRJTHS-FMDGEEDCSA-N 0.000 description 3
• 238000010586 diagram Methods 0.000 description 3
• 229950006780 n-acetylglucosamine Drugs 0.000 description 3
• 108010072866 Prostate-Specific Antigen Proteins 0.000 description 2
• 102000007066 Prostate-Specific Antigen Human genes 0.000 description 2
• 101100315399 Caenorhabditis elegans tsp-14 gene Proteins 0.000 description 1
• 102000051366 Glycosyltransferases Human genes 0.000 description 1
• 108700023372 Glycosyltransferases Proteins 0.000 description 1
• 102000002493 N-Acetylglucosaminyltransferases Human genes 0.000 description 1
• 108010093077 N-Acetylglucosaminyltransferases Proteins 0.000 description 1
• 108010061952 Orosomucoid Proteins 0.000 description 1
• 102000012404 Orosomucoid Human genes 0.000 description 1
• 230000006696 biosynthetic metabolic pathway Effects 0.000 description 1
• 238000001962 electrophoresis Methods 0.000 description 1
• 239000011259 mixed solution Substances 0.000 description 1
• 230000007017 scission Effects 0.000 description 1
• 239000000243 solution Substances 0.000 description 1