JP2020019018A - 画像操作電気力を用いてバイオ粒子を選別する装置及びその作動方法 - Google Patents
画像操作電気力を用いてバイオ粒子を選別する装置及びその作動方法 Download PDFInfo
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Abstract
【解決手段】画像操作電気力によってバイオ粒子を選別するための装置は、第1基板、第2基板、流体チャネル、1つ以上の感光層及び入口孔を含む。第1基板は、第1導電性電極を有し、第2基板は、第2導電性電極を有する。第2導電性電極は、第1導電性電極と対向して配置される。流体チャネルは、第1導電性電極と第2導電性電極との間に配置される。感光層は、第1導電性電極の表面と第2導電性電極の表面のうちの少なくとも一方にコンフォーマルに配置される。入口孔は、第1導電性電極及び第1基板内に配置され、この場合、入口孔は、流体チャネルに近い第1開口部及び流体チャネルから離れた第2開口部を含み、第1開口部の表面積は、第2開口部の表面積よりも大きい。
【選択図】図4
Description
Claims (16)
- 画像操作電気力によってバイオ粒子を選別するための装置であって:
第1導電性電極を備える第1基板と;
第2導電性電極を備える第2基板であって、前記第2導電性電極が前記第1導電性電極と対向して配置される前記第2基板と;
前記第1導電性電極と前記第2導電性電極との間に配置される流体チャネルと;
前記第1導電性電極の表面と第2導電性電極の表面のうちの少なくとも一方にコンフォーマルに配置される1つ以上の感光層と;
前記第1導電性電極及び前記第1基板内に配置される入口孔であって、該入口孔は、前記流体チャネルに近い第1開口部及び前記流体チャネルから離れた第2開口部を含み、前記第1開口部の表面積が、前記第2開口部の表面積よりも大きい、前記入口孔と;
を備える、装置。 - 前記第1導電性電極及び前記第1基板内に配置される出口孔を更に備え、該出口孔は、前記流体チャネルに近い第3開口部及び前記流体チャネルから離れた第4開口部を備え、前記第3開口部の表面積は、前記第4開口部の表面積よりも大きい、
請求項1に記載の装置。 - 前記第1基板内に配置され、前記入口孔の前記第2開口部及び前記出口孔の前記第4開口部にそれぞれ接続される、少なくとも2つの円筒形の貫通孔を更に備える、
請求項2に記載の装置。 - 前記第2導電性電極及び前記第2基板内に配置される出口孔を更に備え、該出口孔は、前記流体チャネルに近い第3開口部及び前記流体チャネルから離れた第4開口部を備え、前記第3開口部の表面積は、前記第4開口部の表面積よりも大きい、
請求項1に記載の装置。 - 前記入口孔は、前記第1開口部から前記第2開口部まで徐々に減少する可変の断面積を含み、前記出口孔は、前記第3開口部から前記第4開口部まで徐々に減少する可変の断面積を含む、
請求項2乃至4のいずれか一項に記載の装置。 - 前記第1基板及び前記第2基板は、それぞれ内面を含み、前記第1導電性電極及び前記第2導電性電極は、それぞれ前記内面によって支持される、
請求項1乃至5のいずれか一項に記載の装置。 - 前記感光層のうちの1つは、前記第1導電性電極の前記表面にコンフォーマルに配置され、前記感光層のうちの別の1つは、前記第2導電性電極の前記表面にコンフォーマルに配置される、
請求項1乃至6のいずれか一項に記載の装置。 - 前記流体チャネルは、メインチャネル及びサイドチャネルを含み、該サイドチャネルの一端は、前記メインチャネルに接続される、
請求項1乃至7のいずれか一項に記載の装置。 - 前記流体チャネルは、湾曲したメインチャネル及びサイドチャネルを含み、該サイドチャネルの一端は、前記湾曲したメインチャネルの頂点に接続される、
請求項1乃至7のいずれか一項に記載の装置。 - 前記流体チャネルは、前記サイドチャネルの別の端部に接続される収集チャネルを更に含み、前記サイドチャネルのチャネル幅に対する前記メインチャネルのチャネル幅の比は1:1〜10:1の間であり、前記収集チャネルのチャネル幅に対する前記サイドチャネルのチャネル幅の比は1:1〜1:10の間である、
請求項8又は9に記載の装置。 - 前記流体チャネルは、前記流体チャネルの内部のイオンを前記流体チャネルの外に拡散させるように構成される半透性チャネルである、
請求項1乃至10のいずれか一項に記載の装置。 - 前記第1基板と前記第2基板との間に配置される中間層を更に含み、前記流体チャネルは、前記中間層内に配置され、前記中間層は生体適合性材料で作られる、
請求項1乃至11のいずれか一項に記載の装置。 - 請求項1乃至7のいずれか一項に記載の装置を作動させる方法であって、前記流体チャネルは、メインチャネルと、該メインチャネルに接続されるサイドチャネルとを含み、当該方法は:
(a)複数のバイオ粒子を含む液体を、入口孔を通して前記メインチャネルに提供するステップと;
(b)前記バイオ粒子が前記メインチャネルの選別領域へ流れるときに前記バイオ粒子を識別するステップと;
(c)前記バイオ粒子が識別された後に、前記液体の流量を減少させるか前記液体の流れを停止するステップと;
(d)ステップ(c)の後に前記バイオ粒子を配置するステップと;
(e)前記選別領域上に光ゾーンを照射するステップと;
(f)前記選別領域上に、暗いゾーンを有する光パターンを照射するステップであって、該光パターンが前記バイオ粒子の少なくとも1つと重なり、前記暗いゾーンが、該暗いゾーンに隣接する領域の輝度よりも暗い輝度を有するステップと;
(g)前記光パターンと重なっているバイオ粒子を移動させるように、前記光パターンを移動させるステップと;
を含む方法。 - ステップ(e)において、前記光ゾーンの領域は、前記メインチャネルの領域の半分よりも大きく、当該方法は、
(h)前記光ゾーンが前記選別領域上に照射されるとき、前記暗いゾーンの領域を拡大するステップ;
を更に含む、請求項13に記載の方法。 - (i)前記暗いゾーンの外側エッジが前記光ゾーンのエッジに到達するまで、前記暗いゾーンの領域を拡大するステップ、
を更に含む、請求項13又は14に記載の方法。 - 前記流体チャネルは、前記サイドチャネルに接続される収集チャネルを更に含み、当該方法は、
(j)前記光パターンと重なっている前記バイオ粒子を前記収集チャネルに移動させるステップ、
を更に含む、請求項13乃至15のいずれか一項に記載の方法。
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