JP2019536985A - キャピラリーゾーン電気泳動/エレクトロスプレーデバイスにおける電流逆転の緩和のための方法及び装置 - Google Patents
キャピラリーゾーン電気泳動/エレクトロスプレーデバイスにおける電流逆転の緩和のための方法及び装置 Download PDFInfo
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- 238000005515 capillary zone electrophoresis Methods 0.000 title claims description 9
- 238000000034 method Methods 0.000 title description 7
- 230000000116 mitigating effect Effects 0.000 title description 3
- 238000000926 separation method Methods 0.000 claims abstract description 72
- 238000002347 injection Methods 0.000 claims abstract description 27
- 239000007924 injection Substances 0.000 claims abstract description 27
- 238000001962 electrophoresis Methods 0.000 claims abstract description 21
- 239000003792 electrolyte Substances 0.000 claims abstract description 17
- 239000007921 spray Substances 0.000 claims description 21
- 238000004949 mass spectrometry Methods 0.000 claims description 4
- 238000001818 capillary gel electrophoresis Methods 0.000 claims description 3
- 238000000533 capillary isoelectric focusing Methods 0.000 claims description 3
- 238000001012 micellar electrokinetic chromatography Methods 0.000 claims description 3
- 238000001649 capillary isotachophoresis Methods 0.000 claims description 2
- 238000002330 electrospray ionisation mass spectrometry Methods 0.000 abstract description 3
- 238000000738 capillary electrophoresis-mass spectrometry Methods 0.000 abstract 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 23
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- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 12
- 239000000872 buffer Substances 0.000 description 11
- CZGUSIXMZVURDU-JZXHSEFVSA-N Ile(5)-angiotensin II Chemical compound C([C@@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC=1NC=NC=1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC=1C=CC=CC=1)C([O-])=O)NC(=O)[C@@H](NC(=O)[C@H](CCCNC(N)=[NH2+])NC(=O)[C@@H]([NH3+])CC([O-])=O)C(C)C)C1=CC=C(O)C=C1 CZGUSIXMZVURDU-JZXHSEFVSA-N 0.000 description 10
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44743—Introducing samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D59/00—Separation of different isotopes of the same chemical element
- B01D59/38—Separation by electrochemical methods
- B01D59/42—Separation by electrochemical methods by electromigration; by electrophoresis
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
- B01L9/06—Test-tube stands; Test-tube holders
- B01L9/065—Test-tube stands; Test-tube holders specially adapted for capillary tubes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/24—Extraction; Separation; Purification by electrochemical means
- C07K1/26—Electrophoresis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44782—Apparatus specially adapted therefor of a plurality of samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82B—NANOSTRUCTURES FORMED BY MANIPULATION OF INDIVIDUAL ATOMS, MOLECULES, OR LIMITED COLLECTIONS OF ATOMS OR MOLECULES AS DISCRETE UNITS; MANUFACTURE OR TREATMENT THEREOF
- B82B1/00—Nanostructures formed by manipulation of individual atoms or molecules, or limited collections of atoms or molecules as discrete units
-
- C—CHEMISTRY; METALLURGY
- C25—ELECTROLYTIC OR ELECTROPHORETIC PROCESSES; APPARATUS THEREFOR
- C25B—ELECTROLYTIC OR ELECTROPHORETIC PROCESSES FOR THE PRODUCTION OF COMPOUNDS OR NON-METALS; APPARATUS THEREFOR
- C25B7/00—Electrophoretic production of compounds or non-metals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00178—Special arrangements of analysers
- G01N2035/00237—Handling microquantities of analyte, e.g. microvalves, capillary networks
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/4473—Arrangements for investigating the separated zones, e.g. localising zones by electric means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/72—Mass spectrometers
- G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
- G01N30/724—Nebulising, aerosol formation or ionisation
- G01N30/7266—Nebulising, aerosol formation or ionisation by electric field, e.g. electrospray
Abstract
Description
L=(P×S×D2)/(3200×C) (1)
を用いて推定された。
本願は、“CE Electrospray Distal-End Power Supply”と題されて2016年9月26日付けで出願された米国特許出願第62/400036に基づく優先権を主張する非仮出願である。なお、優先権の基礎となる先の出願は、その全文を参照により本願に援用される。
本発明は、アメリカ国立衛生研究所(NIH)によって発注された契約番号R01GM096767の下で政府支援を受けてされたものである。政府は、本発明において一定の権利を有している。
Claims (12)
- 広範囲の電気泳動条件でのサンプル解析のための装置であって、
放出部及びエレクトロスプレー・インターフェイスを備えたエレクトロスプレーと、
注入端及び遠位端を有してサンプルを運ぶよう構成される分離キャピラリーであり、該分離キャピラリーの前記注入端は、バックグラウンド電解液を含む容器に挿入され、前記遠位端は、前記エレクトロスプレー・インターフェイス内に螺入され、前記インターフェイスと対になるような大きさ及び形状にされる、前記分離キャピラリーと、
前記注入端及び増幅器へ電気的に接続される第1電源と、
前記増幅器と前記遠位端との間に位置付けられ、電流が前記遠位端へ流れることを可能にするよう構成される少なくとも1つの第1ダイオードと、
前記遠位端と接地との間に位置付けられ、前記接地への電流フローを可能にするよう構成される第2ダイオードと
を有する装置。 - 前記エレクトロスプレーは、質量分析入口部に近接して位置する放出部を更に有する、
請求項1に記載の装置。 - 前記分離キャピラリーは、次のもの:キャピラリーゾーン電気泳動、キャピラリーゲル電気泳動、キャピラリー等電点電気泳動、キャピラリー等速電気泳動又はミセル動電クロマトグラフィのうちの1つによって前記サンプルを分離するよう構成される、
請求項1に記載の装置。 - 増幅器が、輸送容器から供給される輸送電解液とともに輸送キャピラリーを有するシースへ接続される、
請求項1に記載の装置。 - 増幅器が、前記分離キャピラリーの前記遠位端の周りに同心円状に位置する導電素子へ接続される、
請求項1に記載の装置。 - 前記注入端と前記遠位端との間の電位は、11kVから19kVの間である、
請求項2に記載の装置。 - 前記注入端と前記遠位端との間の電位は、19kVよりも大きい、
請求項2に記載の装置。 - 前記ダイオードは整流ダイオードである、
請求項1に記載の装置。 - LED光源を更に有する
請求項1に記載の装置。 - 少なくとも1つの更なる電源を更に有し、
前記第1電源及び前記少なくとも1つの更なる電源は、夫々互いから電気的に絶縁される、
請求項1に記載の装置。 - 前記第2ダイオードと前記接地との間に位置付けられる抵抗を更に有し、該抵抗は、電流逆転シナリオにおいて前記電流フローを制御する、
請求項1に記載の装置。 - 広範囲の電気泳動条件でのサンプル解析のための装置であって、
出力部を有し、サンプルを運ぶ分離手段と、
インターフェイスで前記分離手段の前記出力部へ接続され、放出部を更に有し、前記サンプルをイオン化するイオン化手段と、
前記イオン化手段の前記放出部に近接して位置し、前記サンプルを解析する手段と、
電流が電源に流れ込むことを防ぐ保護手段と
を有し、
前記電源は、電圧差を供給するよう前記分離手段及び前記イオン化手段へ動作上接続される、
装置。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201662400036P | 2016-09-26 | 2016-09-26 | |
US62/400,036 | 2016-09-26 | ||
PCT/US2017/053520 WO2018058131A1 (en) | 2016-09-26 | 2017-09-26 | Methods and apparatus for mitigation of current reversal in capillary zone electrophoresis-electrospray devices |
Publications (1)
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JP2019536985A true JP2019536985A (ja) | 2019-12-19 |
Family
ID=61685232
Family Applications (1)
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JP2019516140A Pending JP2019536985A (ja) | 2016-09-26 | 2017-09-26 | キャピラリーゾーン電気泳動/エレクトロスプレーデバイスにおける電流逆転の緩和のための方法及び装置 |
Country Status (5)
Country | Link |
---|---|
US (1) | US20180088080A1 (ja) |
EP (1) | EP3516386A4 (ja) |
JP (1) | JP2019536985A (ja) |
CN (1) | CN109791132A (ja) |
WO (1) | WO2018058131A1 (ja) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2017095813A1 (en) | 2015-11-30 | 2017-06-08 | Intabio, Inc. | Devices and methods for sample characterization |
JP7333326B2 (ja) | 2018-01-29 | 2023-08-24 | インタバイオ, エルエルシー | 試料の特性評価のための装置、方法およびキット |
US11285484B2 (en) | 2019-08-12 | 2022-03-29 | Intabio, Llc | Multichannel isoelectric focusing devices and high voltage power supplies |
US11340200B2 (en) * | 2020-03-08 | 2022-05-24 | The Board Of Regents Of The University Of Oklahoma | Electrospray assisted capillary device for processing ultra low-volume samples |
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JPH06249833A (ja) * | 1993-03-01 | 1994-09-09 | Hitachi Ltd | 質量分析計 |
US20140305801A1 (en) * | 2011-10-27 | 2014-10-16 | Beckman Coulter, Inc. | Improved capillary electrophoresis-electrospray ionization-mass spectrometry system |
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2017
- 2017-09-26 US US15/716,149 patent/US20180088080A1/en not_active Abandoned
- 2017-09-26 JP JP2019516140A patent/JP2019536985A/ja active Pending
- 2017-09-26 WO PCT/US2017/053520 patent/WO2018058131A1/en active Application Filing
- 2017-09-26 CN CN201780059442.5A patent/CN109791132A/zh active Pending
- 2017-09-26 EP EP17854139.7A patent/EP3516386A4/en not_active Withdrawn
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Also Published As
Publication number | Publication date |
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CN109791132A (zh) | 2019-05-21 |
WO2018058131A1 (en) | 2018-03-29 |
US20180088080A1 (en) | 2018-03-29 |
EP3516386A1 (en) | 2019-07-31 |
EP3516386A4 (en) | 2020-09-02 |
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