JP2019502098A5 - - Google Patents

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Publication number
JP2019502098A5
JP2019502098A5 JP2018522661A JP2018522661A JP2019502098A5 JP 2019502098 A5 JP2019502098 A5 JP 2019502098A5 JP 2018522661 A JP2018522661 A JP 2018522661A JP 2018522661 A JP2018522661 A JP 2018522661A JP 2019502098 A5 JP2019502098 A5 JP 2019502098A5
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JP
Japan
Prior art keywords
sample
methods
sample buffer
swab
surfactant
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JP2018522661A
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Japanese (ja)
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JP7126445B2 (en
JP2019502098A (en
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Priority claimed from PCT/US2016/059928 external-priority patent/WO2017079167A1/en
Publication of JP2019502098A publication Critical patent/JP2019502098A/en
Publication of JP2019502098A5 publication Critical patent/JP2019502098A5/ja
Priority to JP2021162839A priority Critical patent/JP2022008815A/en
Application granted granted Critical
Publication of JP7126445B2 publication Critical patent/JP7126445B2/en
Priority to JP2023035914A priority patent/JP2023085281A/en
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Claims (13)

生物学的物質または環境物質を含む試料を捕集するステップと、
スワブを使用して、前記試料と接触するステップと、
捕集された前記試料を有する前記スワブを、試料緩衝液に入れ、これにより前記試料の少なくとも一部を前記試料緩衝液に移動するステップと、
前記試料をフィルタに通すステップと
を含み、
２分を超える前処理ステップが存在せず、
前記試料緩衝液が、前記試料緩衝液の体積で少なくとも１０％の量の界面活性剤を含む、試料捕集方法。 A step of collecting a sample containing organisms biological substances or environmental substance,
Using a swab to contact the sample;
Placing the swab having the collected sample in a sample buffer, thereby transferring at least a portion of the sample to the sample buffer; and
Look including the step of passing the sample to the filter,
There is no pretreatment step of more than 2 minutes,
The sample collection method, wherein the sample buffer comprises a surfactant in an amount of at least 10% by volume of the sample buffer. 前記物質が、３ｃＰ以上の粘度を有する物質である、請求項１に記載の方法。The method of claim 1, wherein the substance is a substance having a viscosity of 3 cP or more. 前記物質が、痰である、請求項２に記載の方法。The method of claim 2, wherein the substance is sputum. 前記試料緩衝液の中の前記試料にプロテアーゼを接触させるステップをさらに含む、請求項１に記載の方法。 Further comprising the step of contacting the protease with the sample in the sample buffer, methods who claim 1. 前記スワブが、フロックドスワブである、請求項１に記載の方法。 The swab is a floc de swab, methods who claim 1. 前記捕集するステップが、ピペッティングせずに、前記試料を試料容器から捕集するステップを含み、試料の吸収および放出の容量が、１５０〜１０００μＬである、請求項１に記載の方法。 Said step of collecting is, without pipetting, comprising the step of collecting the sample from the sample container, the capacity of the absorption of the sample and release a 150～1000MyuL, methods who claim 1. 前記試料緩衝液が、前記試料緩衝液の体積で１２％〜１８％の範囲の量の界面活性剤を含む、請求項１に記載の方法。 The sample buffer comprises a surfactant in an amount ranging from 12% to 18% by volume of the sample buffer, methods who claim 1. 前記試料緩衝液が、前記試料緩衝液の体積で１５％のトリトン−Ｘを含む、請求項１に記載の方法。 The sample buffer comprises Triton -X 15% by volume of the sample buffer, methods who claim 1. 前記フィルタに通すステップが、プロテアーゼが存在する中で、フィルタを通して前記試料を引き込むステップを含む、請求項１に記載の方法。 Step through the filter, in which the protease is present, comprising the step of pulling the sample through the filter, method who claim 1. 前記試料が、試料マトリクスおよび病原体を含み、前記スワブが、前記病原体を前記試料緩衝液中に選択的に放出しながら、前記試料マトリクスを保持する、請求項１に記載の方法。 Said sample comprises a sample matrix and pathogens, said swab, said while the pathogen was selectively released into the sample buffer, the sample matrix to hold, methods who claim 1. 前処理ステップのためのホールド時間が存在しない、請求項１に記載の方法。 There is no hold time for the pretreatment step, methods who claim 1. 前記試料が、痰であり、前記痰が、前記入れるステップの前に、加熱処理またはＤＴＴ処理を施されない、請求項１に記載の方法。 The sample is a sputum, the sputum is prior to said placing step, not subjected to heat treatment or DTT treatment, methods who claim 1. 前記試料緩衝液が、非イオン性界面活性剤、イオン性界面活性剤、双性イオン界面活性剤、およびこれらの任意の組合せからなる群から選択された界面活性剤を含む、請求項１に記載の方法。 The sample buffer comprises a surfactant selected from the group consisting of a nonionic surfactant, an ionic surfactant, a zwitterionic surfactant, and any combination thereof. method towards.
JP2018522661A 2015-11-02 2016-11-01 Sample preparation for difficult sample types Active JP7126445B2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2021162839A JP2022008815A (en) 2015-11-02 2021-10-01 Sample preparation for difficult sample types
JP2023035914A JP2023085281A (en) 2015-11-02 2023-03-08 Sample preparation for difficult sample type

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562249592P 2015-11-02 2015-11-02
US62/249,592 2015-11-02
PCT/US2016/059928 WO2017079167A1 (en) 2015-11-02 2016-11-01 Sample preparation for difficult sample types

Related Child Applications (1)

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JP2021162839A Division JP2022008815A (en) 2015-11-02 2021-10-01 Sample preparation for difficult sample types

Publications (3)

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JP2019502098A JP2019502098A (en) 2019-01-24
JP2019502098A5 true JP2019502098A5 (en) 2019-12-12
JP7126445B2 JP7126445B2 (en) 2022-08-26

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JP2021162839A Pending JP2022008815A (en) 2015-11-02 2021-10-01 Sample preparation for difficult sample types
JP2023035914A Pending JP2023085281A (en) 2015-11-02 2023-03-08 Sample preparation for difficult sample type

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Country Status (13)

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US (2) US11175205B2 (en)
EP (2) EP3353278B1 (en)
JP (3) JP7126445B2 (en)
KR (1) KR20180080241A (en)
CN (2) CN108473931B (en)
AU (1) AU2016350734B2 (en)
BR (1) BR112018008331B1 (en)
CA (1) CA3003172A1 (en)
CL (1) CL2018001156A1 (en)
ES (1) ES2862224T3 (en)
HK (1) HK1252952A1 (en)
SG (1) SG11201802946WA (en)
WO (1) WO2017079167A1 (en)

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