JP2019154307A5 - - Google Patents
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- Publication number
- JP2019154307A5 JP2019154307A5 JP2018044951A JP2018044951A JP2019154307A5 JP 2019154307 A5 JP2019154307 A5 JP 2019154307A5 JP 2018044951 A JP2018044951 A JP 2018044951A JP 2018044951 A JP2018044951 A JP 2018044951A JP 2019154307 A5 JP2019154307 A5 JP 2019154307A5
- Authority
- JP
- Japan
- Prior art keywords
- injection pipette
- injection
- pipette
- egg
- tip
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000002347 injection Methods 0.000 claims description 245
- 239000007924 injection Substances 0.000 claims description 245
- 235000013601 eggs Nutrition 0.000 claims description 141
- 210000000170 Cell Membrane Anatomy 0.000 claims description 70
- 239000011521 glass Substances 0.000 claims description 50
- 210000000805 Cytoplasm Anatomy 0.000 claims description 28
- 210000000681 Sperm Head Anatomy 0.000 claims description 28
- 230000037250 Clearance Effects 0.000 claims description 16
- 230000035512 clearance Effects 0.000 claims description 16
- 241000124008 Mammalia Species 0.000 claims description 10
- 238000007913 intrathecal administration Methods 0.000 claims 1
- 239000012528 membrane Substances 0.000 description 29
- 210000004681 Ovum Anatomy 0.000 description 20
- 210000004027 cells Anatomy 0.000 description 17
- 241000282898 Sus scrofa Species 0.000 description 15
- 230000000694 effects Effects 0.000 description 11
- 238000005452 bending Methods 0.000 description 10
- 210000002257 embryonic structures Anatomy 0.000 description 10
- 230000004083 survival Effects 0.000 description 10
- 241000282887 Suidae Species 0.000 description 9
- 230000029803 blastocyst development Effects 0.000 description 8
- 238000007796 conventional method Methods 0.000 description 8
- 238000000338 in vitro Methods 0.000 description 8
- 238000000520 microinjection Methods 0.000 description 8
- 210000004340 Zona Pellucida Anatomy 0.000 description 7
- 230000018109 developmental process Effects 0.000 description 7
- 239000007788 liquid Substances 0.000 description 7
- 238000010586 diagram Methods 0.000 description 5
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 5
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 5
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 5
- 238000005498 polishing Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 210000002459 Blastocyst Anatomy 0.000 description 3
- RVZRBWKZFJCCIB-UHFFFAOYSA-N Perfluorotributylamine Chemical compound FC(F)(F)C(F)(F)C(F)(F)C(F)(F)N(C(F)(F)C(F)(F)C(F)(F)C(F)(F)F)C(F)(F)C(F)(F)C(F)(F)C(F)(F)F RVZRBWKZFJCCIB-UHFFFAOYSA-N 0.000 description 3
- 230000004913 activation Effects 0.000 description 3
- 230000004720 fertilization Effects 0.000 description 3
- 210000004508 polar body Anatomy 0.000 description 3
- 206010011732 Cyst Diseases 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N D-Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 241000282412 Homo Species 0.000 description 2
- 208000000509 Infertility Diseases 0.000 description 2
- 210000004940 Nucleus Anatomy 0.000 description 2
- 210000003138 Sperm Tail Anatomy 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 230000013080 embryo development ending in birth or egg hatching Effects 0.000 description 2
- 230000013144 embryo development ending in seed dormancy Effects 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000001963 growth media Substances 0.000 description 2
- 230000036512 infertility Effects 0.000 description 2
- 231100000535 infertility Toxicity 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 210000004952 Blastocoel Anatomy 0.000 description 1
- 210000001161 Embryo, Mammalian Anatomy 0.000 description 1
- 230000030120 acrosome reaction Effects 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000002068 genetic Effects 0.000 description 1
- 230000036244 malformation Effects 0.000 description 1
- 239000002609 media Substances 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000003287 optical Effects 0.000 description 1
- 230000000149 penetrating Effects 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2018044951A JP6862624B2 (ja) | 2018-03-13 | 2018-03-13 | 卵細胞質内注入法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2018044951A JP6862624B2 (ja) | 2018-03-13 | 2018-03-13 | 卵細胞質内注入法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2019154307A JP2019154307A (ja) | 2019-09-19 |
| JP2019154307A5 true JP2019154307A5 (fr) | 2021-02-04 |
| JP6862624B2 JP6862624B2 (ja) | 2021-04-21 |
Family
ID=67992114
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018044951A Active JP6862624B2 (ja) | 2018-03-13 | 2018-03-13 | 卵細胞質内注入法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP6862624B2 (fr) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6870178B2 (ja) * | 2019-02-19 | 2021-05-12 | プライムテック株式会社 | 卵細胞質内注入法 |
| CN112553057A (zh) * | 2020-12-11 | 2021-03-26 | 东蕴医疗科技(上海)有限公司 | 一种用于压电式单精子注射的显微操作针及其制作方法 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0690770A (ja) * | 1991-03-29 | 1994-04-05 | Shimadzu Corp | マイクロマニピュレータ用微小器具 |
| JP3878331B2 (ja) * | 1998-06-10 | 2007-02-07 | 株式会社東海理化電機製作所 | 力センサ付きマイクロマニピュレータ |
| WO2003048786A2 (fr) * | 2001-11-30 | 2003-06-12 | Bristol-Myers Squibb Company | Configurations de pipettes et jeux de pipettes pour mesurer des proprietes electriques cellulaires |
| JP2005348690A (ja) * | 2004-06-14 | 2005-12-22 | Olympus Corp | 物質導入装置 |
-
2018
- 2018-03-13 JP JP2018044951A patent/JP6862624B2/ja active Active
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