JP2019147848A - Make-up compositions, cosmetic compositions, compositions for joint protection, compositions - Google Patents

Abstract

To provide highly safe compositions useful for anti-aging of the skin, joint protection, and the like by effectively inhibiting the expression of MMP-13.SOLUTION: The composition of the invention contains amino sugar, and a plant containing flavonoids. Preferably, the plant containing flavonoids is one or more kinds selected from a Pinus, Kaempferia parviflora, and Fallopia sachalinensis. Also preferably, the amino sugar is glucosamine. The composition of the invention is useful as make-up compositions, cosmetic compositions, compositions for joint protection, and the like by effectively inhibiting the silencing action of MMP-13 percutaneously or orally.SELECTED DRAWING: Figure 1

Description

  The present invention relates to a cosmetic composition, a cosmetic composition, a joint protection composition, and a composition containing an amino sugar and a flavonoid-containing plant.

  It is known that the skin leads to so-called skin aging such as wrinkles and sagging due to various factors such as loss of oil and collagen in addition to the influence of ultraviolet rays. The structure of the skin is divided into three layers of “epidermis”, “dermis” and “subcutaneous tissue” from the top layer. The “stratum corneum” at the top of the epidermis is on the outermost side of the skin and is exposed to various external stimuli such as outside air and ultraviolet rays. Skin troubles such as wrinkles, stains, kusumi, tarmi, rough skin, and aging phenomena appear due to various factors such as immunity decline due to aging, changes in the external environment such as temperature, ultraviolet rays, external stimulation, and excessive intake of foreign substances.

  In recent years, research has progressed and it has become clear that the cause of wrinkle formation is due to fiber reduction and degeneration of the dermal matrix such as collagen and elastin. As a factor for inducing this, the involvement of matrix metalloprotease (Japanese name: matrix metalloproteinase, hereinafter referred to as “MMPs”) has been pointed out.

  MMPs is a general term for extracellular matrix degrading enzymes having zinc (II) ions in the active site. In addition, MMPs are known to have subfamilies such as collagenase (MMP-1 and 13), gelatinase (MMP-2 and 9), stromelysin (MMP-3 and 10) due to differences in structure and substrate specificity. Yes. Of these, the main substrates of MMP-13 are fibrillar collagens (type I, type II, type III) and gelatins, proteoglycans, cytokines and other components of the extracellular matrix.

  It is known that the expression of MMPs such as MMP-13 is enhanced by external stimuli such as ultraviolet rays, thereby decomposing components of the extracellular matrix of the skin (Patent Document 1, Non-Patent Document 1). For this reason, suppressing the production of MMP-13 is effective for preventing skin wrinkles and improving elasticity.

  The degradation of cartilage by MMPs is known to be deeply related to osteoarthritis (sometimes called degenerative osteoarthritis, hypertrophic osteoarthropathy, etc.). It has high degrading activity against type II collagen and is considered to be an important substrate degrading enzyme for cartilage degeneration in osteoarthritis (Non-patent Document 2). In chondrocytes in joints and the like, it is known that expression of MMP-13 is enhanced by various inflammatory cytokinins such as interleukin 1 beta (hereinafter also referred to as “IL1b”) (Non-patent Document 3). MMP-13 is also known to be overexpressed in rheumatoid arthritis and osteoarthritis. Therefore, suppressing the expression of MMP-13 is useful for joint protection such as prevention of decrease in collagen in the cartilage of joints, prevention and improvement of diseases related to cartilage collagen degradation such as osteoarthritis, etc. It is effective for.

  In recent years, as a substance that suppresses the expression of MMP-13, it has been proposed to use a naturally-derived material in consideration of safety when taken orally or percutaneously. As such a material, for example, Non-Patent Document 1 describes an olive leaf extract. Patent Document 3 describes agar, agarose and / or agarooligosaccharide.

JP 2008-156349 A Special table 2011-528013 gazette JP 2011-213707 A

Yoshiyuki Kimura, “Carcinogenesis suppression by UVB irradiation of natural drugs and its mechanism” [online], [November 21, 2014 search], Internet <URL: http://kaken.nii.ac.jp/pdf /2010/seika/jsps/16301/20590700seika.pdf> Ken Okazaki et al., “Control of gene expression in cartilage degeneration in rheumatoid arthritis and osteoarthritis” Fukuoka Medical Journal 103 (9): 175-181, 2012 Takehiko Matsushita, “Role of SIRT1 in chondrocytes”, [online], [searched on November 21, 2014], Internet <URL: http://kaken.nii.ac.jp/pdf/2010/seika/mext/ 14501 / 21791393seika.pdf>

As described above, the expression suppressing action of MMP-13 of various materials is known.
However, the action of these materials is not sufficiently high, and a technique that exhibits a much higher MMP-13 expression suppressing action is required.
Therefore, an object of the present invention is to provide a composition that is highly safe and effective in preventing skin aging, protecting joints, and the like by effectively suppressing the expression of MMP-13.

The present invention provides a cosmetic composition containing an amino sugar and a flavonoid-containing plant.
The present invention provides a cosmetic composition containing an amino sugar and a flavonoid-containing plant.
The present invention provides a joint protective composition containing an amino sugar and a flavonoid-containing plant.
The present invention provides a composition containing an amino sugar and a flavonoid-containing plant.

  According to the present invention, a composition that is highly safe and effective for at least one action selected from an anti-aging action and a joint protecting action of skin can be obtained by effectively suppressing the expression of MMP-13. In addition, the composition of the present invention contributes to the prevention and prevention of locomotive syndrome (musculoskeletal function syndrome) such as a decrease in body balance ability, physical strength and mobility ability by preventing joint pain and limitation of range of motion of the joint. obtain.

FIG. 1 is a graph showing the results of evaluating the MMP-13 gene expression inhibitory effect of the samples of Example 1 and Comparative Examples 1 and 2. FIG. 2 is a graph showing the results of evaluating the MMP-13 gene expression inhibitory effect of the samples of Example 2 and Comparative Examples 3 and 4. FIG. 3 is a graph showing the results of evaluating the MMP-13 gene expression inhibitory effect of the samples of Example 3 and Comparative Examples 5 and 6.

Hereinafter, the present invention will be described based on preferred embodiments thereof. Each of the “cosmetic composition”, “beauty composition”, “joint protection composition”, and “composition” of the present invention contains an amino sugar and a flavonoid-containing plant. The following description applies to any of “cosmetic composition”, “beauty composition”, “joint protection composition”, and “composition” unless otherwise specified.
Hereinafter, these compositions are collectively referred to as “the composition of the present invention”.

(Amino sugar)
The term “amino sugar” as used in the present invention means a compound having a structure in which the hydroxyl group of the sugar is substituted with an amino group, derivatives thereof, and salts thereof. The amino sugar used in the present invention has a chemical structure as long as it exerts the effect of solving the problems of the present invention when applied to mammals including humans in the state of a composition combined with a flavonoid-containing plant (described later). The purity, properties and preparation method are not limited to specific ones. A relatively desirable amino sugar in practicing the present invention exists in a naturally free state or as a structural unit such as a polysaccharide, glycoprotein, glycolipid, and the like. Specifically, for example, glucosamine, mannosamine , Neuraminic acid, galactosamine and derivatives of these amino sugars. Examples of such derivatives that have been confirmed to exist in nature include acylated derivatives such as N-acetylated derivatives, sulfated derivatives such as N-sulfated derivatives and O-sulfated derivatives, and N-glycolylated derivatives. And the like. These can use 1 type (s) or 2 or more types. Of these amino sugars, glucosamines are particularly useful because the effects of the present invention are high. As used herein, glucosamines include glucosamine (Glucosamine, C ₆ H ₁₃ NO ₅ ) or derivatives thereof and salts thereof. In particular, in order to enhance the effect of the present invention, among glucosamines, acylated derivatives of glucosamine are preferable, and N-acetylglucosamine (C ₈ H ₁₅ NO ₆ ) is particularly preferable. The amino sugar used in the present invention is a polysaccharide or glycoprotein containing the above amino sugar as a structural unit, for example, collected from mammals, fish, molluscs, arthropods, fungi, etc. by a conventional method, and subjected to acidic conditions. It can be prepared by subjecting it to conventional methods for separation and purification of saccharides such as chromatography after hydrolysis under the action of an appropriate enzyme. For example, N-acetylglucosamine is produced by, for example, partially hydrolyzing a polysaccharide chitin prepared from crustacean shells such as crabs and shrimps with an acid and further acting on an enzyme such as chitinase. And can be prepared by decomposing. Commercially available preparations (for example, commercially available glucosamine) can also be used.

(Flavonoid-containing plants)
Examples of flavonoids in flavonoid-containing plants include plant secondary metabolites derived from chalcone formed by polymerization of coenzyme A coumarate A and malonyl coenzyme A. Examples of flavonoids include flavones, flavonols, flavanols, proanthocyanidins and the like.

Examples of flavones include 2,3-didehydroflavan-4-one (also referred to as 2-phenylchromone) and derivatives thereof, and 3 ′, 4 ′ of 2,3-didehydroflavan-4-one skeleton, Preferred are those in which a hydroxy group or a methoxy group is bonded to any one or more of the 5th and 7th positions.
Flavonols include 3-hydroxyflavone (also called 3-hydroxy-2-phenylchromen-4-one) and derivatives thereof, and any of 3 ′, 4 ′, 5 and 7 positions of the 3-hydroxyflavone skeleton. Preferred are those having a hydroxy group or a methoxy group bonded to one or more. These may be glycosides in which a sugar is bound to the hydroxy group at the 3-position of the skeleton.
Flavanols include flavan-3-ol and flavan-3,4-diol and derivatives thereof. As these derivatives, those in which a hydroxy group or a methoxy group is bonded to one or more of the 3 ′, 4 ′, 5, and 7 positions of the flavan-3-ol or flavan-3,4-diol skeleton are preferable. Can be mentioned.
Examples of the proanthocyanidins include compounds composed of a condensation polymer having a polymerization degree of 2 or more obtained by condensing flavan-3-ol or flavan-3,4-diol or a derivative thereof by a 4-8 bond. Preferred are those in which a hydroxy group or a methoxy group is bonded to any one or more of the 3 ′, 4 ′, 5, and 7 positions of the constituting flavan-3-ol or flavan-3,4-diol unit.

Among the flavonoids used in the present invention, particularly preferred as flavans are 5-hydroxy-7-methoxyflavone, 5,7-dimethoxyflavone, 5-hydroxy-3,7-dimethoxyflavone, 4 ', 5,7- trimethoxyflavone, 5-hydroxy-3,7,4'-trimethoxyflavone, 3 ', 4', 5,7-tetramethoxyflavone, 5-hydroxy-3,7,3 ', 4'-tetramethoxyflavone, 3,5,7,3 ', 4' -pentamethoxyflavone, 3,5,7-trimethoxyflavone, 3,5,7,4'-tetramethoxyflavone and the like, and flavonols include quercetin, quercetin-3-rutinoside and the like, and flavanols Catechin, epicatechin and the like, and as proanthocyanidins, those obtained by polymerizing catechin or epicatechin and the like, and as anthocyanidins, pelargonidin,, cyanidin, delphinidin, aurantidine, luteolinidine, peonidin, malvidin, petunidin , Europe Blurred, Roshinijin, and the like. In addition, derivatives such as glycosides, esters and polymers of the compounds mentioned above can also be used.

  Examples of the flavonoid-containing plant used in the present invention include citrus fruits such as black ginger and Satsuma mandarin as those containing flavans, and examples of those containing flavonols include giant redbirds and ginkgo biloba, and flavanols. Teas and the like are included as the ones containing potatoes, and pine, grapes, apples, cranberries and the like are included as the ones containing the proanthocyanidins. The composition of the present invention may contain only one of these flavonoid-containing plants, or may contain a mixture of two or more. In the present invention, as the flavonoid-containing plant, it is particularly preferable to use one or more selected from pine, black ginger, and giant redbird. Hereinafter, these preferable materials will be described.

(Pine)
Examples of the pine include plants belonging to the genus Pinaceae, such as Pinus Martina, New Zealand pine, Finnish pine, Epine pine, larch, black pine, Japanese red pine, Japanese pine, Japanese pine, Chinese pine, Japanese pine, Strobe pine, sugar pine, Ryukyu pine, slash pine, caribbean pine, rigid pine, pine pine, daishou, taiwan red pine, banks pine, tsukushima pine, pine pine, white pine, Canadian quebec aneda. Among them, French coastal pine, New Zealand pine, and Finnish pine are preferable, and French coastal pine is particularly preferable. French coastal pine is a marine pine that grows on the Atlantic coast of southern France.

  Examples of pine use sites include bark, leaves, and pine mushrooms, with bark being particularly preferred. As the pine, the bark of French coastal pine is particularly preferable. The bark of French coastal pine contains proanthocyanidins, which are flavonoids, as main components, and also contains organic acids and other physiologically active components. This main component, proanthocyanidins, is known to have a strong antioxidant action to remove active oxygen.

  As the pine, a processed product obtained by subjecting a pine plant body to one or more processes selected from pulverization, extraction, and drying is preferable. Preferable processed products include a pulverized plant obtained by pulverizing a plant, or an extract obtained by extraction with water and / or an organic solvent as it is or after processing the plant. The processing referred to here includes at least one treatment selected from heating, drying, pulverization, and pressing. These workpieces can be used either alone or in combination. Among them, an extract, particularly a pine bark extract (hereinafter referred to as “pine bark extract”) is used from the viewpoint of the remarkable effect of the use of the above-mentioned proanthocyanidins and other physiologically active ingredients and the stability of the quality. Is preferred. As a solvent for obtaining an extract of a pine plant body, water or an organic solvent is selected. When water is used, warm water or hot water is used. The organic solvent used for extraction may be any organic solvent that is usually used, such as methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, butane, acetone, hexane, cyclohexane, propylene glycol, Hydrous ethanol, hydrous propylene glycol, ethyl methyl ketone, glycerin, methyl acetate, ethyl acetate, diethyl ether, dichloromethane, 1,1,1,2-tetrafluoroethane, 1,1,2-trichloroethene and the like are preferably used. These water and organic solvent may be used independently and may be used in combination of 2 or more type. In particular, hot water, hydrous ethanol, hydrous propylene glycol or the like is preferably used.

  The pine bark used in the extraction step is 7 in terms of dry mass when 10 parts by volume of 50-80% ethanol aqueous solution is added to 1 part by weight of pine bark and extracted at 80-85 ° C. for 1 hour. A solid substance (hereinafter referred to as a water-containing ethanol-soluble component) of not less than 10% by mass, preferably not less than 13% by mass, more preferably 13% by mass to 30% by mass is obtained. By using a pine bark containing 7% by mass or more of a water-containing ethanol-soluble component, it is possible to efficiently obtain a pine bark extract having a high effect of the present invention.

  The content of the water-containing ethanol-soluble component in the pine bark can be measured, for example, as follows. First, pine bark having a dry mass of 100 g is pulverized using, for example, a cutter, a slicer, a mill, or a crusher such as a mixer, a juicer, a blender, a mass collider, and the 100 g of the powder contains 50 to 80% by volume of water. 1 L of ethanol is added, and the mixture is refluxed for 1 hour at 80 to 85 ° C. After extraction, separation operations such as centrifugation and filtration are performed to remove insoluble components, and an extract is obtained. From the viewpoint of accurately measuring the water-soluble ethanol-soluble component in the pine bark, it is preferable to perform a re-extraction step in which the extraction operation and the separation operation are repeated once or more for the extraction residue. The obtained extract is freeze-dried or concentrated to dryness under reduced pressure to obtain a dried product, and the mass of the dried product is measured. And the ratio of the mass of the said dried material with respect to the dry mass of the pine bark before extraction is calculated, and content of a hydrous ethanol soluble component is calculated | required.

  The extraction is performed by holding at a predetermined temperature as necessary. From the viewpoint of extraction efficiency, it is preferable to increase the surface area per volume of the pine bark, and crushed materials are particularly preferably used. The crushing treatment of pine bark is not particularly limited, and for example, a crusher employed when measuring the content of the water-soluble ethanol-soluble component in the pine bark described above can be used. In order to increase the crushing efficiency, the pine bark may be crushed by adding water or an organic solvent such as ethanol, methanol, or ethyl acetate. The size of the crushed material is preferably 0.1 to 10 mm, more preferably 0.1 to 5 mm.

  In the extraction, as described above, at least one of water and an organic solvent, that is, water, an organic solvent, or a mixed solvent of water and an organic solvent (hereinafter collectively referred to as an extraction solvent) is used. From the viewpoint of increasing the extraction efficiency, a higher extraction temperature is preferable. For example, when water is used, hot water extraction is preferably performed at 50 to 120 ° C, preferably 70 to 100 ° C. Hot water may be added to the pine bark, and water may be added to the pine bark and then heated. The extraction time can be appropriately determined depending on the extraction temperature. Generally, it is 10 minutes to 24 hours.

  The amount of the extraction solvent can be set in consideration of the extraction efficiency. For example, when water, ethanol, or hydrous ethanol is used as an extraction solvent, the extraction solvent is preferably 3 to 100 parts by mass, more preferably 10 to 50 parts by mass, or more preferably 10 to 50 parts by mass with respect to 1 part by mass of pine bark dry mass. It is preferably set to 3 to 100 parts by volume, more preferably 10 to 100 parts by volume. In addition, when adding water and / or an organic solvent and crushing, the amount of the extraction solvent to be added may be adjusted in consideration of the amount of solvent used for crushing.

  An extraction method for obtaining an extract of a pine plant body is not particularly limited, and for example, a warm extraction method, a supercritical fluid extraction method, or the like is used. For example, a water-ethanol mixed solvent (hydrous ethanol) having a mass ratio of water to ethanol of 1: 1 to 1: 9 is used as an extraction solvent for crushed pine bark. A method of stirring for 0.5 to 6 hours while refluxing at 70 to 85 ° C. using double to 20 times amount is mentioned. In the case of not refluxing, for example, extraction with warming is performed once using the above mixed solvent, and the supernatant is recovered by filtration or the like, and the residue is heated again by adding the above mixed solvent to increase the extraction efficiency. It is possible.

  The supercritical fluid extraction method is a method of performing extraction using a supercritical fluid that is a fluid that exceeds the critical point (critical temperature, critical pressure) of a gas-liquid substance. As the supercritical fluid, carbon dioxide, ethylene, propane, nitrous oxide (laughing gas) or the like is used, and carbon dioxide is preferably used.

  In the supercritical fluid extraction method, an extraction process for extracting a target component with a supercritical fluid and a separation process for separating the target component and the supercritical fluid are performed. In the separation step, any one of extraction separation by pressure change, extraction separation by temperature change, and extraction separation using an adsorbent / absorbent may be performed.

  Moreover, you may perform supercritical fluid extraction by the entrainer addition method. In this method, for example, ethanol, propanol, n-hexane, acetone, toluene, other aliphatic lower alcohols, aliphatic hydrocarbons, aromatic hydrocarbons, and ketones are added to the extraction fluid in an amount of 2 to 20 W / V. It is a method that drastically increases the solubility of the target extract in the extraction solvent or enhances the separation selectivity by performing supercritical fluid extraction using this fluid. It is a method of obtaining a pine bark extract.

  Since the supercritical fluid extraction method can be operated at a relatively low temperature, it can be applied to substances that are altered or decomposed at high temperatures, the advantage that the extraction fluid does not remain, and the recycling of the solvent is possible. There is an advantage that the process and the like can be omitted, and the process becomes simple.

  Moreover, the extraction method from the plant body for obtaining the extract of the pine plant body is not limited to the above-described extraction method, but by a method such as a liquid carbon dioxide batch method, a liquid carbon dioxide reflux method, or a supercritical carbon dioxide reflux method. You may go.

  The extraction method for obtaining the extract of the pine plant body may be a combination of a plurality of extraction methods. By combining a plurality of extraction methods, it is possible to obtain extracts having various compositions.

  For the extraction, for example, any extraction device such as a batch type, a semi-continuous type, or a continuous type may be used.

  Extracts from pine plants, especially bark, should be purified by ultrafiltration or column method or batch method using adsorptive carriers (Diaion HP-20, Sephadex-LH20, chitin, etc.) Is preferable from the viewpoint of safety. Further, if necessary, it may be concentrated or dried by a method such as vacuum concentration or freeze-drying to obtain a liquid, paste, powder (extract powder), fine granule, or granule. The processed pine, such as an extract of pine bark, is preferably in the form of powder, fine particles, or granules. In addition, the pine bark extract may be sterilized or may be further concentrated, dried, and powdered after sterilization. Sterilization, concentration, drying and pulverization are carried out by methods commonly used in the art, and among these, freeze drying, vacuum drying, spray drying, drum drying, shelf drying, and microwave Drying by is preferred. Regarding filtration, in order to perform the filtration process in a short time, it is preferable to filter after adding a filter aid, for example, diatomaceous earth, in this case, the amount of the filter aid to be added is not particularly limited. For example, the amount of diatomaceous earth in the extract is added so as to be about 0.001 g / mL to 0.1 g / mL.

  When pine is used in the present invention, the pine preferably contains proanthocyanidins as one of main components as flavonoids. As described above, proanthocyanidin is a compound group consisting of a condensation polymer having a degree of polymerization of 2 or more having at least one selected from flavan-3-ol, flavan-3,4-diol and derivatives thereof as a structural unit. Say. Proanthocyanidins are powerful antioxidants produced by plants and are intensively contained in plant leaves, bark, fruit peels and seeds. This proanthocyanidin is a substance that cannot be produced in the human body. More preferably, the processed pine product contains 10% by mass or more, 20% by mass or more, 30% by mass or more, and 40% by mass or more of proanthocyanidins.

  The proanthocyanidins contained in the processed pine are particularly preferably proanthocyanidins containing a large amount of a condensation polymer having a low degree of polymerization. The condensation polymer having a low polymerization degree is preferably a condensation polymer having a polymerization degree of 2 to 30 (2 to 30 mer), more preferably a condensation polymer having a polymerization degree of 2 to 10 (2 to 10 mer). Further, a condensation polymer (2 to 4 mer) having a polymerization degree of 2 to 4 is more preferable. Proanthocyanidins that are condensation polymers having a degree of polymerization of 2 to 4 (2 to 4 mer) are particularly easily absorbed into the body. In the present specification, a polymer having a degree of polymerization of 2 to 4 is referred to as an oligomeric proanthocyanidin (hereinafter referred to as “OPC”).

  The processed pine used in the present invention desirably contains OPC in an amount of 10% by mass or more, preferably 20% by mass or more, more preferably 30% by mass or more.

  A pine plant body, particularly a pine bark, is rich in OPC among proanthocyanidins, and is therefore preferably used as a raw material for proanthocyanidins. However, pine plants, particularly pine bark, contain not only proanthocyanidins but also components other than proanthocyanidins. For example, when the processed product of pine is an extract, various organic acids and flavonoids are also extracted by the process of extracting proanthocyanidins from the plant body. Such organic acids and flavonoids are components other than proanthocyanidins. Examples of such organic acids and flavonoids include caffeic acid, fumaric acid, quinic acid, gallic acid, vanillic acid, ferulic acid, and catechins.

(Black ginger)
Kaempferia Parviflora used in the present invention is a plant belonging to the genus Gingidae or Banturmeric that grows naturally in Southeast Asia. It increases energy, enhances nutrition, lowers blood sugar, restores physical strength, improves digestive system, subvaginal zone There are reports of improvement in hemorrhoids, hemorrhoids, nausea, stomatitis, joint pain, and stomach pain. Thus, black ginger is a natural plant that has been ingested by humans for a long time and is highly safe.

  Examples of the use site of the black ginger used in the present invention include roots, leaves, stems, flowers, branches and the like. Among them, preferred is a rhizome. Examples of the black ginger used in the present invention include a processed product obtained by subjecting black ginger to at least one treatment selected from heating, drying, crushing, pressing, and extraction. Specific examples of the processed product of black ginger include a dried product obtained by subjecting black ginger to a drying treatment, a dried powder obtained by subjecting black ginger to drying treatment and pulverization treatment, a cut product obtained by cutting black ginger, or The powder obtained by drying it, juice, and an extract can be mentioned. Here, the extract means an extract obtained by extracting the black ginger or a processed product thereof with a solvent, a diluted solution or a concentrated solution thereof, or a dried product thereof and a powder thereof. In the present invention, the processed article includes all of them.

  The dried powder obtained by subjecting black ginger to drying and crushing is, for example, washed black ginger, sliced black ginger dried in the sun or using a dryer, and then cut as it is or into an appropriate shape and size. The processed product obtained in this manner can be obtained by pulverization using a pulverizer. As the pulverizer, those usually used can be widely used. For example, a pulverizer constituted by a raw material hopper, a pulverizer, a classifier and a product holder can be used.

  An extract of black ginger is obtained by extracting black ginger or a processed product thereof with a solvent. The extract is preferably obtained by subjecting a black ginger to a processed product subjected to a drying treatment, and obtained by subjecting the processed product subjected to a drying treatment after slicing to an extraction treatment. Is preferred. Examples of the solvent used for extraction include lower alcohols such as ethanol, methanol, isopropanol, and butanol, lower esters such as ethyl acetate and methyl acetate, acetone, and a mixed solvent of these with water. It is also effective to use water alone. Among these, it is preferable to use water alone or ethanol alone or a mixed solvent of water and ethanol (so-called water-containing ethanol). When hydrous ethanol is used, the ethanol concentration in the hydrous ethanol is preferably 20% by mass or more and 80% by mass or less, and more preferably 40% by mass or more and 60% by mass or less.

  When a mixed solvent is used as the solvent, for example, an acetone / water (2/8 to 8/2, volume ratio) mixture, an ethanol / water (2/8 to 8/2, volume ratio) mixture, or the like may be used. it can. In the case of ethanol / water, it is preferable to add a solvent having a mass of 2 to 20 times the mass of black ginger rhizome and extract it at room temperature or under heating for about 10 minutes to 48 hours.

  There is no particular limitation on the extraction method to be used, but from the viewpoint of safety and convenience, the extraction method is preferably performed under as mild a condition as possible. For example, pulverize, crush or shred the raw material plant part or its dried product, add 2 to 20 times the mass of the solvent, and leave it at 0 ° C. to the reflux temperature of the solvent for 10 minutes to 48 hours. The extraction is performed under any conditions such as stirring or refluxing. After the extraction operation, separation operations such as filtration and centrifugation are performed to remove insoluble matters. An extract is obtained by diluting and concentrating as necessary. Further, the same operation may be repeated for insoluble matter, and the extract may be used in combination with the previous extract. These extracts may be used after further purification by a purification method commonly used by those skilled in the art.

  The obtained extract can be used as it is or after being concentrated to form a liquid, a concentrate, a paste, or a dried product obtained by further drying these. Drying is performed by a method commonly used by those skilled in the art, such as spray drying, freeze drying, reduced pressure drying, and fluidized drying. Furthermore, it is also possible to use the dried article obtained by the above method by pulverizing it using a known method. Processed products such as black ginger extract are preferably in the form of powder, fine granules, or granules.

  Black ginger used in the present invention is, as a flavonoid, 5-hydroxy-7-methoxyflavone, 5,7-dimethoxyflavone, 5-hydroxy-3,7-dimethoxyflavone, 4 ', 5,7-trimethoxyflavone, 5-hydroxy-3, 7,4'-trimethoxyflavone, 3 ', 4', 5,7-tetramethoxyflavone, 5-hydroxy-3,7,3 ', 4'-tetramethoxyflavone, 3,5,7,3', 4'-pentamethoxyflavone, 3 , 5,7-trimethoxyflavone, 3,5,7,4′-tetramethoxyflavone and the like are preferably contained. In particular, the black ginger used in the present invention preferably contains 0.01 to 50% by mass of 5,7-dimethoxyflavone, more preferably 0.1 to 30% by mass, and more preferably 0.5 to 20% by mass. It is more preferable to include.

(Great Bluebird)
The giant redbird is a perennial plant belonging to the genus Ondate, and its scientific name is Polygonum sachalinense.

  Examples of the site where the giant redbird is used include leaves, stems, buds, roots, rhizomes and the like, and buds are particularly preferred. Buds are also called young shoots and new shoots.

  As the giant redbird, a processed product obtained by subjecting a giant redbird plant to one or more processes selected from pulverization, extraction and drying is preferable. Preferable processed products include a pulverized plant obtained by pulverizing a plant, or an extract obtained by extraction with water and / or an organic solvent as it is or after processing the plant. The processing referred to here includes at least one treatment selected from heating, drying, pulverization, and pressing. These workpieces can be used either alone or in combination. Among them, it is preferable to use an extract, in particular, an extract of the bud of the giant redbird, from the viewpoint of the remarkable effect during use and the stability of the quality. Water or an organic solvent is selected as the solvent for obtaining the extract of the plant of the giant redbird. When water is used, warm water or hot water is used. The organic solvent used for extraction may be any organic solvent that is usually used, such as methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, butane, acetone, hexane, cyclohexane, propylene glycol, Hydrous ethanol, hydrous propylene glycol, ethyl methyl ketone, glycerin, methyl acetate, ethyl acetate, diethyl ether, dichloromethane, 1,1,1,2-tetrafluoroethane, 1,1,2-trichloroethene and the like are preferably used. These water and organic solvent may be used independently and may be used in combination of 2 or more type. In particular, water, hydrous ethanol, hydrous propylene glycol or the like is preferably used.

  In the extraction, as described above, at least one of water and an organic solvent, that is, water, an organic solvent, or a mixed solvent of water and an organic solvent (hereinafter collectively referred to as an extraction solvent) is used. From the viewpoint of increasing the extraction efficiency, a higher extraction temperature is preferable. For example, when water is used, hot water extraction is preferably performed at 50 to 120 ° C, preferably 70 to 100 ° C. In addition, hot water may be added to the bluebird, and water may be added to the bluebird and then heated. The extraction time can be appropriately determined depending on the extraction temperature. Generally, it is 10 minutes to 24 hours.

  It is safe to purify the extract from the plant of the giant redbird by ultrafiltration or the column method or batch method using an adsorptive carrier (Diaion HP-20, Sephadex-LH20, chitin, etc.) From the viewpoint of Further, if necessary, it may be concentrated or dried by a method such as vacuum concentration or freeze-drying to obtain a liquid, paste, powder (extract powder), fine granule, or granule. It is preferable that the processed product of the bluebird, such as the extract of the bluebird, is in the form of powder, fine particles, or granules. In addition, the extract of the giant redbird may be sterilized, or may be further concentrated, dried, and powdered after sterilization. Sterilization, concentration, drying and pulverization are carried out by methods commonly used in the art, and among these, freeze drying, vacuum drying, spray drying, drum drying, shelf drying, and microwave Drying by is preferred.

  When the giant redbird is used in the present invention, the processed product preferably contains quercetin-3-rutinoside as a flavonoid. In particular, the giant redbird used in the present invention preferably contains quercetin-3-rutinoside, preferably 0.1% by mass or more, more preferably 1% by mass or more, and more preferably 10% by mass or more. I like it.

  The composition of the present invention is preferably intended for animals, particularly preferably for mammals, and particularly preferably for humans.

  The composition of the present invention may consist essentially of an amino sugar and a flavonoid-containing plant. The term “consisting essentially of an amino sugar and a flavonoid-containing plant” includes a case in which no component other than the component inevitably mixed in the processing step of an amino sugar and a flavonoid-containing plant is included. It means a case where the total mass of amino sugar and flavonoid-containing plants in the composition is 95% by mass or more. However, the composition of the present invention can contain various other components to be described later in addition to the amino sugar and flavonoid-containing plants, as long as the effects of the present invention are not impaired.

(Content)
From the viewpoint of further enhancing the effect of the present invention, when the composition of the present invention is orally ingested, the content of amino sugar in the composition of the present invention is preferably 10 to 2000 mg, more preferably 100 to 1000 mg, 200 ˜800 mg is more preferred. When the composition of the present invention is orally ingested, the content of amino sugar in the composition of the present invention is preferably 1 to 80% by mass, more preferably 5 to 50% by mass, and further 10 to 30% by mass. preferable.

  From the viewpoint of further enhancing the effect of the present invention, when the composition of the present invention is orally ingested, the content of the flavonoid-containing plant in the composition of the present invention is preferably 1 to 500 mg, preferably 5 to 200 mg in terms of dry mass. Is more preferable, 10-100 mg is still more preferable, and 20-80 mg is especially preferable. When the composition of the present invention is orally ingested, the content of the flavonoid-containing plant in the composition of the present invention is preferably 0.001 to 50% by mass, more preferably 0.01 to 40% by mass in terms of dry mass. Preferably, 0.1-30 mass% is still more preferable.

  From the viewpoint of further enhancing the effect of the present invention, when the composition of the present invention is ingested parenterally, the content of amino sugar in the composition of the present invention is preferably 0.000001 to 100 mg, preferably 0.00001 to 10 mg. Is more preferable, 0.00001 to 1 mg is more preferable, and 0.0001 to 0.1 mg is particularly preferable. When the composition of the present invention is ingested parenterally, the content of amino sugar in the composition of the present invention is preferably 0.000001 to 20% by mass, more preferably 0.00001 to 1% by mass, and 0001-0.1 mass% is still more preferable.

  From the viewpoint of further enhancing the effects of the present invention, when the composition of the present invention is ingested parenterally, the content of the flavonoid-containing plant in the composition of the present invention is preferably 0.000001 to 100 mg by dry mass, 0.00001-10 mg is more preferable, 0.00001-1 mg is still more preferable, and 0.0001-0.1 mg is especially preferable. When parenterally ingesting the composition of the present invention, the content of the flavonoid-containing plant in the composition of the present invention is preferably 0.000001 to 20% by mass, more preferably 0.00001 to 1% by mass in terms of dry mass. Preferably, 0.0001 to 0.1% by mass is more preferable.

  From the standpoint of further enhancing the effects of the present invention, whether the composition of the present invention is taken orally or parenterally, the mass ratio of amino sugar: dry mass of the flavonoid-containing plant ( The former: latter) is preferably 1: 0.0001 to 1000, more preferably 1: 0.001 to 100, still more preferably 1: 0.01 to 10, and 1: 0. It is especially preferable that it is 01-1.

  The composition of the present invention can be used for cosmetic purposes, and in this case, it is a cosmetic composition. The cosmetic composition of the present invention is administered parenterally such as transdermal administration.

  The cosmetic composition of the present invention contains, in addition to the above-mentioned amino sugar and flavonoid-containing plants, other ingredients usually used in quasi-drugs or cosmetics as long as the effects of the cosmetics are not impaired. May be. Examples of such components include water, other medicinal ingredients, other oils, bases, moisturizers, powders, gelling agents, thickeners, surfactants, emulsifiers, anti-inflammatory agents, antioxidants, pH adjusters, chelating agents, thickeners, pigments, fragrances, preservatives, skin aging prevention / improving agents such as collagen, hair growth inhibitors, antiseptics, antifungal agents, UV absorbers, absorption promoters, UV rays A dispersing agent etc. are mentioned. Other medicinal ingredients here include active oxygen scavengers, antioxidants, anti-inflammatory analgesics, antihistamines, antipruritics, bactericides, vitamins, hormones, carotenoids such as vitamin A, vitamin Bs, ascorbic acid , Vitamin E, and derivatives or salts thereof.

  The cosmetic composition of the present invention can be prepared by mixing other components other than amino sugar and flavonoid-containing plants by a commonly used method, and can be prepared as a pharmaceutical, quasi-drug, cosmetic, toiletry product. Can be used. The specific form of the cosmetic is not particularly limited, but cream, cosmetic cream, milky lotion, lotion, serum, lotion, face wash, cleansing, body soap, body shampoo, pack, soap, foundation, lipstick, lip gloss , Blusher, aqueous ointment, oily ointment, ship, gel (gel), skin cosmetics such as bath preparation, shampoo, rinse, conditioner, treatment, styling agent, hair tonic, hair cream, hair conditioner, hair conditioner, soap, It may be a cosmetic for hair such as a perm / color pre- and post-treatment agent, and a product (so-called miscellaneous goods) that is not covered by the Pharmaceutical Affairs Law in a dosage form similar to cosmetics. In addition, it can be locally administered for a long time by a method such as holding or absorbing in a carrier such as ship or gel or a cross-linking agent and applying it to a local area. In addition, administration as eye drops and nasal drops is also possible.

  The composition of the present invention can be used for cosmetic use or joint protection use, in which case it becomes a cosmetic composition or a joint protection composition. The administration form of these compositions may be parenteral administration or oral administration. When the cosmetic composition and the joint protection composition of the present invention are administered parenterally, examples of the ingredients other than the amino sugar and flavonoid-containing plants and dosage forms of these compositions are the cosmetic compositions of the present invention described above. And can be similar. Further, in the case of oral administration, examples of formulation ingredients and dosage forms other than the amino sugar and flavonoid-containing plants of the cosmetic composition of the present invention and joint protection composition may be the same as the oral composition described later. it can. As a method for parenteral administration in these compositions, intravenous administration, intramuscular administration, subcutaneous administration, transdermal administration and the like are used. It is good also as intraperitoneal administration which is one of subcutaneous administration. Further, as an enteral administration, it may be administered directly to the viscera using injection or the like.

  When the composition of the present invention is an orally ingested composition, in the oral composition, in addition to the above-mentioned amino sugar and flavonoid-containing plants, other commonly used components are used as the oral composition. You may contain in the range which does not impair the effect of. Examples of such components include various excipients, binders, brighteners, lubricants, stabilizers, diluents, extenders, thickeners, emulsifiers, antioxidants, pH adjusters, colorants, and fragrances. And additives. The content of other components can be appropriately selected according to the form of the composition of the present invention.

  The composition of the present invention can be formulated into various dosage forms by a known formulation method by adding a pharmaceutically acceptable base material or carrier, if necessary. Examples of the dosage form include: Powder, granule, granule, tablet, rod, plate, block, solid, round, liquid, paste, capsule like hard capsule or soft capsule, caplet, tablet, gel, chewable , Syrup form, stick form and the like.

  The composition of the present invention is derived from natural products and can be safely ingested parenterally and / or orally, and also contains an amino sugar and a flavonoid-containing plant, as is apparent from the description of Examples below. In addition, an inhibitory action on MMP-13 gene expression can be obtained. In particular, the composition of the present invention can suppress the production of MMP-13 in various tissues and cells by ingesting it parenterally and / or orally. For example, by suppressing the production of MMP-13 in the skin, it is possible to prevent a decrease in the amount of collagen in the skin and prevent or improve skin aging such as prevention of skin wrinkles and improvement of elasticity. . In addition, the composition of the present invention is useful for joint protection such as reduction of collagen in the cartilage of the joint by inhibiting the production of MMP-13 in the chondrocytes of the joint, etc., and it is useful for osteoarthritis, rheumatoid arthritis, bone It is effective in preventing and improving diseases related to cartilage collagen degradation such as arthritis. The composition of the present invention is useful as a cosmetic composition or an oral composition capable of exerting any one or more of these actions, and any one or two of these actions. By being able to exhibit the above, it is useful as a beauty composition, a joint protection composition, and the like.

  In particular, the composition of the present invention is useful for protecting the cartilage in a joint or the like and maintaining a healthy state by suppressing the degradation of cartilage collagen through the suppression of the expression of MMP-13. Cartilage protection prevents and ameliorates joint pain, prevents restriction of the range of motion of the joint, and facilitates smooth movement of the joint. Joint pain and limitation of range of motion are said to reduce balance ability, physical strength, and movement ability, and cause so-called locomotive syndrome (motor organ function syndrome). Therefore, the composition of the present invention can prevent and prevent locomotive syndrome (motor organ function syndrome) associated with joint pain and limitation of range of motion, prevent a decrease in balance ability, physical strength, and movement ability, and maintain posture ( (Preventing curling of the back) and keeping the muscles flexible and youthful.

  Hereinafter, the present invention will be described in more detail with reference to examples. However, the scope of the present invention is not limited to such examples. Hereinafter, unless otherwise specified, “%” represents mass%, and “part” represents mass part. Among the following Examples 1 to 3, Example 1 is an example in which an amino sugar and pine are combined, and Example 2 is an example in which an amino sugar and black ginger are combined. Example 3 is an example in which an amino sugar and a bluebird are combined.

[Example 1, Comparative Examples 1 and 2, and Reference Examples 1 and 2]
Various liquids (1) to (4) were prepared as shown in <Test liquid preparation I> below, and the obtained test liquids were subjected to the following <MMP-13 expression test I>.

<Preparation of test solution I>
As a solution of (1), 10% FBS-DMEM medium was used.
(2) Amino sugar-containing liquid N-acetylglucosamine (manufactured by Nacalai Tesque, hereinafter abbreviated as “NAG”) was used as the amino sugar. This was dissolved in a 10% FBS-DMEM medium to a concentration of 10 mg / mL, and after stirring for 1.5 hours by inversion, the filtered one was further serially diluted with the same medium to obtain the final concentration of NAG ( 10% FBS-DMEM media containing 3 times and 6 times the concentration of the following Table 1) were prepared.
(3) Pine-containing liquid As the pine, a pine bark extract (trade name “Flavandenol” (registered trademark, manufactured by Toyo Shinyaku Co., Ltd.), powdered form, containing 30% by mass or more of OPC) was used. This is dissolved in a 10% FBS-DMEM medium so as to have a concentration of 10 mg / mL, and after stirring by inverting for 1.5 hours, the filtered one is further serially diluted with the same medium to obtain a pine bark extract. 10% FBS-DMEM media containing 3 times and 6 times the final concentration (see Table 1 below) were prepared.
(4) Expression inducer-containing solution As a gene expression inducer of MMP-13, an aqueous solution of interleukin 1 beta (hereinafter also referred to as “IL1b”) (Pepro Tech, IL1b concentration 10 μg / mL, hereinafter “IL1b stock solution” Also called). 3 μL of this IL1b stock solution was added to 15 mL of 10% FBS-DMEM medium to prepare an expression inducer-containing solution.

(Water-soluble ethanol-soluble content of pine bark)
The water-soluble ethanol-soluble content of pine bark used for the preparation of flavangenol was 13.2% by mass as measured according to the following method. First, 2 kg of dried pine bark was pulverized to a size of 1 to 5 mm with a mill to obtain a pulverized product, and 10 g was separated from this pulverized product to prepare a sample. To this sample, 100 mL of an aqueous solution containing 80 volume (v / v)% ethanol was added, and reflux extraction was performed at 80 ° C. for 1 hour. Subsequently, it filtered and the filtrate 1 was collect | recovered. For the filtration residue, extraction and filtration were further performed using an aqueous ethanol solution in the same manner as described above to obtain a filtrate twice (referred to as filtrates 2 and 3, respectively). The obtained filtrates 1 to 3 were combined to make an extract, and concentrated to dryness under reduced pressure to obtain a dried product (hereinafter referred to as a hydrous ethanol-soluble component). When the mass of this dried product was measured, it was 1.32 g.

(Manufacturing method of flavangenol)
Flavangenol is prepared according to the following method.
First, 0.5 L of water was added to 100 g of pine bark, and reflux extraction was performed at 95 ° C. or higher for 1 hour. Next, filtration is performed to collect 0.5 L of filtrate (filtrate 4). Further, 0.5 L of water is added to the insoluble matter after filtration and reflux extraction is performed in the same manner as above, followed by filtration. To obtain 0.5 L of filtrate (referred to as filtrate 5). The filtrate 4 and the filtrate 5 were combined to obtain 1 L of pine bark extract. The filtrate was concentrated and dried, and the resulting solid was used as flavangenol.

<MMP-13 expression test I>
Normal human articular chondrocytes (NHAC, Lonza) were seeded on a 24-well plate at 5.4 × 10 ⁴ cells / well and pre-cultured overnight. To the cells, the above solutions (1), (2), (3) and (4) were added in the order described in the amounts shown in Table 1 below. After the addition, the cells were cultured at 37 ° C. for 18 hours in a 5% CO ₂ concentration CO ₂ incubator. Total RNA was extracted from the cultured cells using Seppazole RNA (Nacalai Tesque) according to the attached protocol. Using the obtained total RNA as a template, cDNA was synthesized using a cDNA preparation kit (QIAGEN, product name: Quantitect Reverse Transcription Kit). Reaction obtained in the RT-PCR kit (QIAGEN, product name: Rotor-Gene Green PCR Kit) using 2 μL of the resulting cDNA-containing reaction solution diluted 20-fold with Nuclease free water (QIAGEN) PCR was performed under the following conditions by mixing with 8 μL of the solution. 8 μl of the reaction solution is a mixed solution of 2 × Rotor-Gene SYBR Green PCR Master Mix 5 μL, Primer 1 μL, and Nuclease free water 2 μL. PCR was performed by using Rotor-Gene Q manufactured by QIAGEN and monitoring the amount of PCR product in real time. In the above RT-PCR, GAPDH (housekeeping gene) was used as an internal standard. Specifically, the amount of MMP-13 PCR product / the amount of GAPDH PCR product was used as the gene expression value of MMP-13. Experiments were performed in triplicate to determine the average gene expression value. For the gene expression values of Example 1, Comparative Examples 1 and 2, and Reference Example 1, the ratio when the average value of the gene expression value of MMP-13 of Reference Example 2 was set to 1 was determined as the relative value of gene expression. . The average value of the relative values obtained is shown in FIG.

(RT-PCR conditions)
Hold: 95 ℃ ・ 5min
Cycle: 95 ℃ ・ 5sec → 60 ℃ ・ 10 sec → 72 ℃ ・ 10sec × 45cycle
Melt: 72-99 ℃

(Primer)
-GAPDH: Hs_GAPDH_2_SG QuantiTect Primer Assay (QT01192646)
・ MMP-13: Hs_MMP13_1_SG QuantiTect Primer Assay (QT00001764)

  As is clear from the results shown in FIG. 1, Example 1 combining amino sugar and pine significantly suppressed MMP-13 gene expression induced by the addition of IL1b. On the other hand, in Comparative Examples 1 and 2 in which amino sugar and pine were blended alone, no significant inhibitory action was observed. From this result, it is clear that the composition of the present invention exhibits an excellent inhibitory action on MMP-13 expression by combining amino sugar and pine. It is also clear that the composition of the present invention can effectively prevent a decrease in the amount of accumulated collagen in various connective tissues such as cartilage and skin through the suppression of MMP-13 expression, and is effective for beauty and joint protection. It is.

[Example 2, Comparative Examples 3 and 4, and Reference Examples 5 and 6]
Various liquids (i) to (iv) were prepared as in <Test liquid preparation II> below, and the obtained test liquids were subjected to <MMP-13 expression test II> below.

<Preparation of test solution II>
As the solution (i), 10% FBS-DMEM medium was used.
(Ii) Amino sugar-containing liquid In (2) in <Test liquid preparation I>, the concentration of the prepared amino sugar-containing liquid was set to a concentration four times the final concentration of interest (see Table 2 below). In the same manner as in (2) of <Test Solution Preparation I>.
(Iii) Black ginger-containing liquid A powdery extract (hereinafter, also referred to as “BG”) produced by the following <Black ginger extract production method> was used as black ginger. The obtained powdery extract was dissolved in 10% FBS-DMEM medium so as to have a concentration of 10 mg / mL, and after stirring for 1.5 hours, the filtered one was further serially diluted with the same medium, A 10% FBS-DMEM medium containing the extract at a concentration 4 times the final final concentration (see Table 2 below) was prepared.
(Iv) Expression inducer-containing solution In (4) of <Preparation of test solution I>, except that it was prepared by adding 2.67 μL of IL1b stock solution (10 μg / mL) to 10 mL of 10% FBS-DMEM ( Same as 4).

(Production method of black ginger extract)
The rhizome of black ginger was fragmented and dried to obtain a chip-like rhizome. 300 g of this rhizome chip is weighed and placed in a triangular flask together with 3 L of 60% water-containing ethanol. Allow to stand at room temperature for 24 hours while stirring several times along the way. After filtration under reduced pressure, the remaining chip is immersed again in 3 L of 60% aqueous ethanol and left to stand at room temperature for 24 hours for extraction. This was filtered under reduced pressure to obtain a second extract. The first and second extracts were combined and concentrated under reduced pressure to about 1/6 volume to obtain a stock solution. The obtained stock solution was dried to obtain a powdery extract. The content of 5,7-dimethoxyflavone in the obtained extract was 6% by mass.

<MMP-13 expression test II>
Instead of adding the solutions (1) to (4) above, the above-mentioned solutions (i), (ii), (iii) and (iv) were added to normal human articular chondrocytes in the amounts shown in Table 2 below. A gene expression value was obtained in the same manner as in <MMP-13 expression test I> except that they were added in this order. For the gene expression values of Example 2, Comparative Examples 3 and 4, and Reference Example 3, the ratio when the average expression value of Reference Example 4 was set to 1 was determined as the relative value of gene expression. The average value of the relative values obtained is shown in FIG.

  As is clear from the results shown in FIG. 2, Example 2 combining amino sugar and black ginger significantly suppressed the gene expression of MMP-13 induced by the addition of IL1b. On the other hand, in Comparative Examples 3 and 4 in which amino sugar and black ginger were blended alone, no significant inhibitory action was observed. From this result, it is clear that the composition of the present invention exhibits an excellent inhibitory action on MMP-13 expression by combining amino sugar and black ginger. It is also clear that the composition of the present invention can effectively prevent a decrease in the amount of accumulated collagen in various connective tissues such as cartilage and skin through the suppression of MMP-13 expression, and is effective for beauty and joint protection. It is.

[Example 3, Comparative Examples 5 and 6, and Reference Examples 7 and 8]
Various liquids (a) to (d) were prepared as in <Test liquid preparation III> below, and the obtained test liquids were subjected to <MMP-13 expression test III> below.

<Preparation of test solution III>
As the liquid (a), 10% FBS-DMEM medium was used.
(B) Amino sugar-containing solution In (2) of <Test Solution Preparation I>, the concentration of the prepared amino sugar-containing solution was set to a concentration four times the final concentration (see Table 3 below). In the same manner as in (2) of <Test Solution Preparation I>.
(C)
A powdery extract produced by the following (Production method of European redbird extract) was used as the giant redbird. This was dissolved in 10% FBS-DMEM medium so that the concentration of the bluebird extract was 10 mg / mL, and after stirring for 1.5 hours, the filtered one was further serially diluted with the same medium. A 10% FBS-DMEM medium containing 4 times the final concentration of interest (see Table 3 below) was prepared.
(D) Expression inducer-containing solution 1.35 μL of IL1b stock solution (10 μg / mL) was added to 5 mL of 10% FBS-DMEM.

(Manufactured method of giant redbird extract)
An extract was obtained by extracting and purifying young red buds with hot water. This extract was freeze-dried to obtain a powder. The resulting extract contained quercetin-3-rutinoside.

<MMP-13 expression test III>
Instead of adding the liquids (1) to (4) above, the liquids (a), (b), (c) and (d) above were added to normal human articular chondrocytes in the amounts shown in Table 3 below. A gene expression value was obtained in the same manner as in <MMP-13 expression test I> except that they were added in this order. For the gene expression values of Example 3, Comparative Examples 5 and 6, and Reference Example 5, the ratio when the average value of the gene expression values of Reference Example 6 was 1 was defined as the relative value of gene expression. The average value of the relative values obtained is shown in FIG.

  As is apparent from the results shown in FIG. 3, Example 3 in which an amino sugar and a bluebird were combined greatly suppressed the gene expression of MMP-13 induced by the addition of IL1b. On the other hand, in Comparative Examples 5 and 6 in which amino sugars and redbirds were blended alone, the gene expression inhibitory action of MMP-13 was greatly inferior. From this result, it is clear that the composition of the present invention exerts a synergistic inhibitory action on MMP-13 expression by combining an amino sugar and a bluebird. It is also clear that the composition of the present invention can effectively prevent a decrease in the amount of accumulated collagen in various connective tissues such as cartilage and skin through the suppression of MMP-13 expression, and is effective for beauty and joint protection. It is.

(Formulation example 1: lotion)
Based on 100 parts by mass as a whole, pine bark extract 0.005 parts by mass, N-acetylglucosamine 0.005 parts by mass, glycerin 10 parts by mass, diglycerin 3 parts by mass, 1,3-butylene glycol 12 parts by mass, pentylene Glycol 3 parts, sodium hyaluronate 0.1 part, citric acid 0.01 part, sodium citrate 0.02 part, xanthan gum 0.1 part, methylparaben 0.15 part, carbomer 0.2 part Part of the mixture, 0.03 part by mass of sodium hydroxide and the remaining part of water were mixed to prepare the composition of the present invention in the form of lotion.

(Formulation example 2: shampoo)
Based on 100 parts by weight as a whole, 0.001 part by weight of pine bark extract, 0.001 part by weight of N-acetylglucosamine, 7.5 parts by weight of sodium laureth sulfate, 4.2 parts by weight of cocamidopropyl betaine, 3 parts by weight of cocamide DEA Parts, 1,3-butylene glycol 0.1 parts by weight, polyquaternium-10 0.225 parts by weight, citric acid 0.15 parts by weight, sodium citrate 0.05 parts by weight, phenoxyethanol 0.9 parts by weight and water balance Mixed to prepare the composition of the present invention in the form of a shampoo.

(Formulation example 3: soap)
100 parts by weight as a whole, 1 part by weight of black ginger powder, 1 part by weight of N-acetylglucosamine, 2 parts by weight of glycerin, 1 part by weight of olive oil, 0.1 part by weight of EDTA-4 sodium, 0.2 part by weight of etidronate 4 sodium The composition of the present invention was prepared in the form of a soap by mixing and solidifying the part and the rest of the soap base.

(Formulation example 4: emulsion)
The total weight is 100 parts by weight, with 0.05 parts by weight of extract, 0.001 parts by weight of N-acetylglucosamine, 3 parts by weight of sucrose fatty acid ester, 12 parts by weight of glycerin, 6 parts by weight of squalane, 24 parts by weight of dimethyl silicone oil , 1 part by weight of polypropylene glycol, 0.06 part by weight of thickener, 0.2 part by weight of phenoxyethanol, 5 parts by weight of ethanol, 0.01 part by weight of sodium hydroxide, and the balance of purified water are mixed in the form of an emulsion. Inventive compositions were prepared.

(Formulation Example 5: Cosmetic cream)
100 parts by weight as a whole, black ginger extract 0.03 parts by weight, N-acetylglucosamine 0.1 part by weight, squalane 15.0 parts by weight, octyldodecyl myristate 4.0 parts by weight, hydrogenated soybean phospholipid 0 0.2 parts by mass, butyl alcohol 2.4 parts by mass, hardened oil 1.5 parts by mass, stearic acid 1.5 parts by mass, lipophilic glyceryl monostearate 1.5 parts by mass, polyglyceryl monostearate 0.5 parts by mass Part, 0.8 parts by weight of behenyl alcohol, 0.7 parts by weight of polyglyceryl monomyristate, 0.3 parts by weight of beeswax wax, 0.1 parts by weight of d-δ-tocopherol, 0.3 parts by weight of methylparaben, C10-30 alkyl modified Carboxyvinyl polymer 0.2 parts by mass, Carboxyvinyl polymer 0.1 parts by mass, 1,3-butanediol 18 0 parts by weight 0.1 parts by weight of sodium hydroxide and a mixture of purified water balance, the composition of the present invention was prepared in the manner of cosmetic creams.

(Formulation Example 6: Packing agent)
5. 100 parts by weight of the whole, 0.1 parts by weight of the giant redbird extract, 0.01 parts by weight of N-acetylglucosamine, 20.0 parts by weight of polyvinyl alcohol, 5.0 parts by weight of glycerol, 20.0 parts by weight of ethanol, kaolin The composition of the present invention was prepared in the form of a pack agent by mixing 0 part by mass, 0.2 part by mass of a preservative, 0.1 part by mass of a fragrance, and the remainder of purified water.

(Formulation Example 7: Tablet)
The whole is 100 parts by weight, black ginger powder 5 parts by weight, N-acetylglucosamine 10 parts by weight, vitamin B ₁ 5 parts by weight, vitamin B ₆ 12 parts by weight, calcium stearate 4 parts by weight, maltose 30 parts by weight and hydroxypropyl cellulose The composition of the present invention was prepared in the form of a tablet by mixing and tableting the remainder.

(Formulation Example 8: Granules)
Based on 100 parts by weight as a whole, 5 parts by weight of pine bark extract, 8 parts by weight of powdered extract, 5 parts by weight of N-acetylglucosamine, 5 parts by weight of black ginger extract, 10 parts by weight of lactose, 1 part by weight of calcium stearate and crystals The composition of the present invention was prepared in the form of granules by mixing and granulating the cellulose residue.

(Formulation example 9: capsule)
100 parts by weight as a whole, 10 parts by weight of a giant redbird extract, 10 parts by weight of N-acetylglucosamine, 10 parts by weight of black ginger extract, 8 parts by weight of lecithin and the rest of olive oil The composition of the present invention was prepared in the form of a capsule by encapsulating in a capsule shell.

(Composition example 10: Liquid preparation) 100 parts by weight of the whole, 1 part by weight of pine bark extract, 1 part by weight of N-acetylglucosamine, 1 part by weight of vitamin B _1, 10 parts by weight of fructose glucose liquid sugar, 1 part by weight of citric acid ,
Sodium benzoate 0.02 parts by mass, flavor preparation 2 parts by mass, sucralose 0.05 part by mass, acesulfame potassium 0.03 part by mass, and purified water balance are prepared to prepare the composition of the present invention in the form of a liquid agent did.

Claims (2)

  A composition for protecting or maintaining articular cartilage, comprising glucosamines and one or more plants selected from a plant containing proanthocyanidins and a plant containing dimethoxyflavone.   A cosmetic composition comprising glucosamines and one or more plants selected from proanthocyanidin-containing plants and dimethoxyflavone-containing plants.