JP2019144207A - Reaction vessel, and fluorescence measuring device using reaction vessel - Google Patents

Abstract

To recognize an installation direction of a reaction vessel to a fluorescence measuring device.SOLUTION: A reaction vessel has a plurality of wells for storing an analyte on the same plane or on the same straight line. A specific well other than a well which is the center of a point object among wells on a well plate is used as a well for position discrimination where a fluorescent dye is arranged. In the reaction vessel, the position of the well for position discrimination can be specified by utilizing fluorescence emitted from the fluorescent dye, and thereby an orientation of the reaction vessel can be recognized.SELECTED DRAWING: Figure 1

Description

  The present invention relates to a reaction container having a plurality of wells for containing a specimen containing a gene, and a fluorescence measuring apparatus for measuring fluorescence from the specimen contained in each well of the reaction container.

  In the field of genetic testing, a reaction vessel such as a well plate having a plurality of wells or a connecting tube is often used (for example, see Patent Document 1). The specimen is accommodated in a well of a reaction container together with a reagent such as a PCR reaction reagent, and the reaction container is installed in a measuring apparatus such as a real-time PCR apparatus. A real-time PCR device measures fluorescence intensity from a gene in a sample labeled with a fluorescent dye in parallel with gene amplification processing that amplifies a specific gene in the sample, and uses the measurement results to perform various analysis processes. Can be performed. An apparatus for measuring the fluorescence intensity from a gene in a sample labeled with a fluorescent dye in this way is generically called a fluorescence measurement apparatus.

  A 96-well plate, which is one of the commercially available reaction vessels, has 96 wells arranged in a matrix of 8 columns vertically and 12 columns horizontally. In a fluorescence measuring apparatus that handles such wells, the position of each well of the well plate is represented by a code (for example, A to H) indicating the position of the vertical column and a code (for example, 1 to 1) indicating the position of the horizontal column. 12) are identified by identification codes (for example, A10 (10th from the left end of the A column), C7 (7th from the left end of the C column), etc.).

  Before starting the measurement with the fluorescence measuring device, the user can register information on the sample such as the sample ID of the sample contained in each well of the reaction container and the analysis item to be performed in the device. is there. Information about the sample input by the user is registered in association with the identification code of the well in which the sample is stored. Thereby, the analysis result about the sample accommodated in each well can be obtained in a state associated with the sample ID accommodated in each well.

Special table 2007-526767

  Since reaction containers such as well plates have symmetrical shapes in the vertical and horizontal directions, it is difficult for the user to determine the orientation of the reaction container. For this reason, there is a frame having an identification code (for example, A to H in the vertical direction, 1 to 12 in the horizontal direction, etc.) indicating the position of the well in a frame having a width of about 10 mm provided on the outer periphery of the reaction vessel. . In addition, there is a case in which a cut indicating the direction of the reaction vessel is provided at one corner of the outer peripheral frame, for example, near the upper right A12 well).

  However, the identification code printed on the frame of the reaction vessel is formed simultaneously with resin molding of the reaction vessel and is the same color as the reaction vessel, so that the visibility is poor. In addition, since the position of the cut provided in the frame differs depending on the manufacturer, there is a possibility that the user may misidentify the direction of the reaction container. As a result, the installation direction of the reaction container to the fluorescence measuring device may be wrong without the user's knowledge.

  When the user sets the reaction container in the fluorescence measuring device in the wrong direction, the position where the well in which each sample is stored is arranged is the position information of the sample registered in the apparatus (identification code indicating the position of the well) Therefore, there is a problem that the analysis process different from the analysis process designated by the user is performed for each sample, or the analysis result for each sample is mistaken.

  Therefore, an object of the present invention is to make it easy to correctly recognize the result even when the installation direction of the reaction container with respect to the fluorescence measuring apparatus is wrong.

  The reaction container according to the present invention is a well plate or a connecting tube having a plurality of wells for containing a sample on the same plane or the same straight line, and is a well other than the center well of the point target among the plurality of wells Fluorescent dyes are arranged in specific wells. The well in which the fluorescent dye is arranged is referred to as a position determination well. That is, in the reaction container of the present invention, the position of a specific well or the direction of the reaction container is determined using fluorescence emitted from the fluorescent dye.

  As the fluorescent dye, a fluorescent dye having a fluorescent wavelength suitable for the purpose can be selected and used from fluorescent dyes such as fluorescein, fluorescein amidite, rhodamine and the like. Normally, a fluorescent dye with a fluorescence wavelength different from the fluorescent dye for detecting amplified DNA contained in the reaction reagent is selected, but if it can be discriminated by an increase in the fluorescence baseline, a fluorescent dye with the same fluorescence wavelength can be selected. Good.

  Examples of the reaction reagent include an RT-PCR reaction reagent that measures the expression level of a gene and a reagent for SNP analysis that determines a genotype. In addition to SYBR (registered trademark, product of ThermoFisher Scientific) Green I, fluorescent dyes that emit fluorescence by binding to nucleic acids and probes for detecting nucleic acids include fluorescent dyes that color when such reaction reagents are added. A labeled fluorescent dye can be used.

  The fluorescent dye may be disposed in the position determination well in a solid phase so as to be dissolved when a reaction reagent is added to the position determination well.

  When the reaction container of the present invention has a plurality of wells arranged in a matrix within the plane, wells provided at corners of the plane or in the vicinity thereof are the position determination wells. preferable. If it does so, it will become easy to distinguish the positional relationship of the well for position determination in the plane of a well plate, and the operativity of the user who adds a reaction reagent will improve.

  The fluorescence measuring apparatus according to the present invention includes a reaction container installation unit for installing the reaction container of the present invention described above, and from each well provided in the reaction container installed in the reaction container installation unit. A measurement unit for measuring the intensity of light having a wavelength to be measured, the measurement unit having a function of detecting fluorescence emitted from a fluorescent dye disposed in a position determination well of the reaction vessel Is done.

  The fluorescence measurement apparatus according to the present invention further includes a position determination well of the reaction container when the wavelength of fluorescence emitted from the fluorescent dye of the position determination well of the reaction container is the same as the wavelength of the measurement target. A measurement configured to correct the measurement value for the position determination well based on the difference between the baseline of the measurement value by the measurement unit and the baseline of the measurement value by the measurement unit for the other well A value correction unit can be provided. Thereby, even if the fluorescent dye arranged in the position determination well of the reaction container emits fluorescence having the same wavelength as the wavelength to be measured, the influence of the fluorescence emitted from the fluorescent dye on the measurement is removed. be able to.

  As described above, the reaction container of the present invention includes a position determination well at a specific position, and a fluorescent dye is disposed in the position determination well. When the fluorescence intensity from each well of the reaction vessel is measured by the measurement unit, the baseline of fluorescence and fluorescence intensity from the fluorescent dye that exists only in the position determination well where the fluorescent dye is placed in advance is that of other wells. Also gets higher. Therefore, in the fluorescence measuring apparatus of the present invention, it is possible to measure the difference between the fluorescence or measurement value baseline for the position determination well and the measurement value baseline for other wells.

  The fluorescence measuring apparatus according to the present invention can cope with the case where the wavelength of fluorescence emitted from the fluorescent dye in the position determination well of the reaction container is different from the wavelength of the measurement target.

  In this way, by measuring the fluorescence from the fluorescent dye arranged in the position determination well, the fluorescent dye arranged in the position determination well has the same wavelength as that used for the measurement of the specimen. In some cases, the position of the position determination well of the reaction container installed in the reaction container setting section is compared by comparing the measurement value baseline of the position determination well with the baseline of the measurement values of the other wells. Can be specified.

  Therefore, it is preferable that the fluorescence measuring apparatus of the present invention includes a well position specifying unit configured to specify the position of the position determination well based on the measurement value of each well by the measuring unit. . If the position of the position determination well can be identified, the information can be used to automatically determine the installation direction of the reaction container.

  In addition, since the position of the position determination well in the reaction container is determined, if the position is stored in the apparatus as the original position (original position) of the position determination well, the function of the well position specifying unit described above can be obtained. It is also possible to confirm whether or not the reaction vessel is installed in the correct orientation in the reaction vessel installation part.

  Therefore, in the fluorescence measuring apparatus of the present invention, it is determined whether or not the original position of the position determination well defined in advance and the actual position of the position determination well specified by the well position specifying unit coincide with each other. A well position determination unit configured as described above may be further provided.

  The well position determination unit may be configured to issue a warning to the user when the actual position of the well position specifying unit and the original position do not match. Then, the user can easily recognize an error in the installation direction of the reaction container.

  By the way, information (specimen information) related to the sample accommodated in each well of the reaction container is registered in the apparatus in association with the position of the well in which each sample is accommodated. In the present application, such a part that stores specimen information is referred to as a specimen information storage unit. If the reaction container is installed in the wrong direction, the arrangement of each sample on the reaction container installation part registered in advance in the device is different from the arrangement of each sample actually installed on the reaction container installation part. Resulting in.

  On the other hand, if the function of said well position specific | specification part is used, an apparatus can recognize how the reaction container is installed in the reaction container installation part. Therefore, in the fluorescence measurement device of the present invention, when the well position determining unit determines that the actual position of the well position specifying unit and the original position do not match, the well position specifying unit specifies the well position. Based on the actual position of the well for position determination, the sample information stored in the sample information storage unit is associated with the position of the well in which each sample is actually stored. It is preferable to provide a correspondence correction unit that corrects the correspondence between the sample information in the information storage unit and the position of the well. Then, even if the user makes a mistake in installing the reaction container in the reaction container installation unit, the apparatus automatically recognizes it and corrects the correspondence between the specimen information registered in advance and the position of the well. Therefore, the situation that the analysis result for the specimen is mistaken does not occur.

  In the reaction container according to the present invention, the well provided at a specific position other than the well that is the center of the dot target among all the wells is a position determination well in which a fluorescent dye is arranged. The direction of the reaction vessel can be easily determined using fluorescence emitted from the dye. The reaction container can be used not only for PCR measurement by a PCR measurement apparatus but also for measurement of a melting curve of a specimen after PCR processing is performed by the PCR measurement apparatus. Furthermore, it can be applied not only to PCR but also to antigen-antibody reaction and enzyme reaction using fluorescence detection.

  In the fluorescence measurement device according to the present invention, the measurement unit for measuring light of the wavelength to be measured from each well of the reaction vessel detects fluorescence from the fluorescent dye arranged in the position determination well. Since it has a function, the position of the reaction container position determination well can be specified.

It is a perspective view which shows one Example of a well plate. It is a top view of the Example. It is a schematic sectional block diagram which shows one Example of a fluorescence measuring apparatus. It is a figure which shows an example of the detection signal waveform of the normal well by the same Example, and the well for position determination. It is a flowchart which shows an example of operation | movement of the Example. It is a top view of a well plate which shows the arrangement | sequence of each well when a well plate is installed in the wrong direction. It is a flowchart which shows the other example of operation | movement of the Example.

  Below, one Example of a reaction container and a fluorescence measuring apparatus is described using drawing.

  First, an example of a reaction vessel will be described with reference to FIG.

  The reaction container 2 of this embodiment is a container in which a plurality of containers 4 are arranged and connected in the same plane. Each container 4 includes a well 6 that is open at the top. The well 6 is for accommodating a specimen and a reaction reagent therein. In this embodiment, the wells 6 are arranged in a matrix in 8 rows and 12 rows. In order to identify the position of each well 6, the left end and the upper end of the upper surface of the well plate 2 are respectively provided with identification codes A to H indicating vertical columns and identification codes 1 to 12 indicating horizontal columns. Has been.

  Among the wells 6 provided in the reaction vessel 2, fluorescent dyes 8 are arranged in the wells 6 provided at specific positions other than the center of the point target. In this embodiment, the fluorescent dye 8 is disposed in the well 12 of the H12 (12th from the left of the H row) located at the lower right end of the reaction vessel 2. Hereinafter, the well 6 in which the fluorescent dye 8 is arranged is referred to as a position determination well 6 s in order to distinguish it from other wells 6. In addition, the position of the position determination well 6s where the fluorescent dye 8 is disposed may be a position of a well other than the center of the point target in all the wells 6.

  The fluorescent dye 8 disposed in the position determination well 6s is solidified by drying, for example. The fluorescent dye 8 preferably has a property of being dissolved and colored when a reaction reagent is added to the position determination well 6s, but it is not necessarily required to be colored by the addition of the reaction reagent. Any substance that emits fluorescence when irradiated with excitation light may be used. The fluorescent dye 8 does not necessarily need to be solid-phased, and may be sealed in the position determination well 6s by some means. As the fluorescent dye, SYBR (registered trademark) Green I, SYBR (registered trademark) Gold, EvaGreen (registered trademark), or the like can be used.

  When the reaction reagent is added to the position determination well 6s by fixing the fluorescent dye having the property of being colored when the reaction reagent is added to the position determination well 6s, as shown in FIG. As shown, since the position determination well 6s provided at a specific position is colored, the orientation of the reaction vessel 2 can be grasped visually. When a fluorescent dye 8 that does not add a reaction reagent and emits fluorescence when irradiated with excitation light is used, it is not always necessary to add a reaction reagent to the position determination well 6s. By measuring the fluorescence intensity obtained by irradiating with excitation light, the position of the position determination well 6s can be determined.

  Next, an example of the fluorescence measuring apparatus using the reaction container 2 will be described with reference to FIG.

  The fluorescence measuring apparatus includes a reaction container installation unit 9 for installing the reaction container 2. The reaction vessel installation unit 9 is made of a heat conductive metal material, and the temperature is adjusted by, for example, a Peltier element or a heater. The reaction container installation unit 9 is provided with a recess 10 for accommodating each container 4 of the reaction container 2.

  A measurement unit 12 is provided above the reaction vessel installation unit 9. The measurement unit 12 includes an optical sensor 14. The optical sensor 14 has a light emitting element that emits excitation light vertically downward and a light receiving element for detecting fluorescence from the specimen excited by the excitation light.

  The measurement unit 12 arranges the optical sensor 14 at a position immediately above each well 6 so as to sequentially scan each well 6 of the reaction container 2 during measurement, and the measurement target from the sample accommodated in each well 6 This is for measuring the intensity of light of the wavelength. The measurement unit 12 has a function of detecting fluorescence from the fluorescent dye disposed in the position determination well 6 s of the reaction vessel 2 by the optical sensor 14. The detection signal obtained by the optical sensor 14 is taken into the arithmetic processing unit 18. The arithmetic processing unit 18 is realized by a dedicated computer or a general-purpose personal computer. A display unit 30 is connected to the arithmetic processing unit 18. The display unit 30 can be realized by a liquid crystal display, for example.

  The arithmetic processing unit 18 has a function of performing various analysis processes based on the detection signal from the optical sensor 14. The analysis processing performed by the arithmetic processing unit 18 includes, for example, absolute quantitative analysis for determining the concentration of a measurement sample, relative quantitative analysis for determining a relative expression level with respect to a gene, and SNP analysis for determining a genotype. The user can set an analysis process to be performed on the sample to be measured as a measurement condition.

  In addition, the measurement part 12 may be provided so that it may move in the horizontal surface under the reaction container installation part 9. In that case, measurement light is measured at the bottom of the recess of the reaction vessel installation unit 9 so that the light of the wavelength to be measured from the sample accommodated in each well 6 can be measured by the optical sensor 14 of the measurement unit 12. An opening is provided.

  The arithmetic processing device 18 includes a measurement value correction unit 20, a well position specifying unit 22, a well position determination unit 24, a correspondence relationship correction unit 26, and a specimen information storage unit 28. The measurement value correction unit 20, the well position specifying unit 22, the well position determination unit 24, and the correspondence relationship correction unit 26 are obtained when an arithmetic element such as a CPU provided in the arithmetic processing device 18 executes a predetermined program. It is a function. The sample information storage unit 28 is a function realized by a partial area of the storage device provided in the arithmetic processing unit 18.

  The measurement value correcting unit 20 is configured to detect the position determination well of the well plate 2 when the wavelength of the fluorescence emitted from the fluorescent dye disposed in the position determination well 6s of the reaction container 2 is the same as the wavelength to be measured. It is configured to correct the measurement value of the intensity of the light having the wavelength to be measured of the specimen contained in 6s. When the wavelength of the fluorescence emitted from the fluorescent dye disposed in the position determination well 6s of the reaction container 2 is the same as the wavelength to be measured, the fluorescence intensity from each well 6 of the reaction container 2 is measured by the optical sensor 14. When measured, as shown in FIG. 4, the baseline of the fluorescence intensity of the position determination well 6 s in which the fluorescent dye is previously arranged becomes higher than that of the normal well 6. The measurement value correcting unit 20 obtains a difference ΔS between the fluorescence intensity baseline of the normal well 6 and the fluorescence intensity baseline of the position determination well 6s, and calculates the difference ΔS from the measurement value of the sample in the position determination well 6s. By subtracting, the measurement value of the sample in the position determination well 6s is corrected. Thus, the fluorescence intensity of the specimen can be measured using the position determination well 6s in the same manner as the normal well 6. As the baseline of the normal well 6, for example, the average value of the baselines of all the wells 6 other than the position determination well 6s among the wells 6 provided in the reaction vessel 2 can be used.

  The well position specifying unit 22 is configured to specify the actual position of the position determination well 6s on the reaction vessel setting unit 9 by comparing the measured values of the fluorescence intensity for each well 6 of the reaction vessel 2. . When the wavelength of the fluorescence emitted from the fluorescent dye arranged in the position determination well 6s of the reaction container 2 is the same as the wavelength of the measurement object, the position determination well 6s to which the fluorescent dye is fixed in advance is measured. The measured value of the intensity of the light having the wavelength to be measured is larger than the measured value of the intensity of the light having the wavelength to be measured for the other wells 6. For this reason, by comparing the fluorescence intensities measured by the optical sensor 14 for each well 6, it is possible to specify the position where the position determination well 6 s is arranged on the reaction container setting unit 9. Further, when the wavelength of the fluorescence emitted from the fluorescent dye disposed in the position determination well 6s of the reaction container 2 is different from the wavelength to be measured, the well 6 in which the light having the wavelength emitted from the fluorescent dye is detected. The position of the position determination well 6s can be specified by searching for.

  The well position determination unit 24 is configured to determine whether or not the position of the position determination well 6s specified by the well position specifying unit 22 is correctly arranged at a specified position. In the arithmetic processing unit 18, a specific position where the position determination well 6s is provided in the reaction vessel 2, for example, the position of H12 is registered in advance as a specified position. This specified position is a position where the position determination well 6s should be disposed when the reaction container 2 is installed in the reaction container installation unit 9 in the correct orientation. The well position determination unit 24 is configured to determine whether or not the actual position of the position determination well 6s specified by the well position specifying unit 22 matches a specified position.

  If the reaction container 2 is installed in the correct orientation in the reaction container installation unit 9, the actual position of the position determination well 6s specified by the well position specifying unit 22 matches the specified position. However, as shown in FIG. 6, when the direction of the reaction vessel 2 is wrong, the position determination well 6s is located at a position (position A1 in FIG. 6) different from the specified position (position H12). Therefore, the actual position of the position determination well 6s specified by the well position specifying unit 22 does not match the specified position. Thereby, it can be determined automatically by the apparatus side whether the installation direction of the reaction container 2 is correct.

  The correspondence correction unit 26 is configured to correct the correspondence between the sample information regarding each sample stored in each well 6 of the reaction container 2 and the position of the well 6 in which each sample is stored.

  Before the measurement is started, the user registers, for example, the sample ID of the sample stored in each well 6 and the measurement condition for each sample as sample information. The sample information of each sample is registered in association with the position of the well 6 used for measuring each sample. The sample information registered in the apparatus by the user is stored in the sample information storage unit 28.

  However, as shown in FIG. 6, when the user places the reaction container 2 in the wrong direction and installs it in the reaction container installation unit 9, the actual position on the reaction container installation unit 9 of the well 6 in which each specimen is stored is This is different from the information stored in the sample information storage unit 28. Referring to FIG. 6, the well 6 provided at the position A1 of the reaction vessel 2 is arranged at the position H12 on the reaction vessel setting portion 9, and the well 6 provided at the position A2 of the reaction vessel 2 is On the reaction container installation part 9, it will be arrange | positioned in the position of H11.

  Therefore, the correspondence correction unit 26 determines in what direction the reaction container 2 is in the reaction container installation unit 9 based on the position of the position determination well 6s specified by the well position specification unit 22 on the reaction container installation unit 9. The sample of each sample stored in the sample information storage unit 28 is determined so as to be associated with the actual position on the reaction container installation unit 9 of the well 6 in which each sample is stored. Correct the information.

  An example of the operation of this embodiment will be described with reference to FIG.

  First, the user inputs sample information, and the sample information of each sample is stored in the sample information storage unit 28 in association with the position of the well 6 in which each sample is accommodated (step S1). Thereafter, the user installs the reaction container 2 in the reaction container installation unit 9 (step S2). Thus, the measurement preparation is completed. Before the reaction container 2 is installed in the reaction container installation unit 9, only the position determination well 6 is colored when the user adds a reagent to each well 6 of the well plate 2. It is easy to recognize the direction.

  When measurement preparation is complete, the user performs some action to indicate that. As an operation indicating the completion of measurement preparation, in addition to an operation to input the fact to the arithmetic processing unit 18, an operation to close a cover (not shown) covering the upper part of the reaction vessel installation unit 9 is included. When the user performs such an operation, the well position specifying unit 20 recognizes that the measurement preparation is completed, and scans all the wells 6 of the reaction container 2 using the optical sensor 14 of the measurement unit 12. Then, the position of the position determination well 6s is specified (step S3).

  After the position of the position determination well 6s is specified, the well position determination unit 24 determines whether or not the position of the position determination well 6s is at a specified position (step S4). Starts measurement (step S7). In the measurement, the measurement value correction unit 20 corrects the measurement value of the sample in the position determination well 6s.

  If the position of the position determination well 6s is not at the specified position, the well position determination unit 24 issues a warning to the user (step S5). Examples of the warning include, for example, displaying the fact on the display unit 30 connected to the arithmetic processing unit 18 and generating a warning sound. It is not always necessary to give a warning to the user.

  When the position of the position determination well 6s is not at the specified position, the correspondence correction unit 26 matches the actual position on the reaction container setting unit 9 of the well 6 in which each sample is stored. The sample information of each sample stored in the information storage unit 28 is corrected (step S6). Thereafter, measurement is started (step S7).

  Not limited to the above, the correspondence correction unit 26 corrects the correspondence between the measurement data and the sample information so that the measurement data obtained by the measurement is correctly associated with the sample after the measurement is completed. It may be like this.

  The correspondence correction unit 26 is not an essential component. Therefore, even when the installation direction of the reaction container 2 is wrong, the correspondence relationship between the sample information and the well position information does not have to be automatically corrected.

  An example of the operation of the fluorescence detection apparatus that does not include the correspondence correction unit 26 will be described with reference to FIG.

  Also in the example of FIG. 7, after the user inputs sample information (step S11) and the reaction container 2 is set in the reaction container setting unit 9 (step S12), the well position specifying unit 20 sets the position of the position determination well 6s. Is specified (step S13). After the position of the position determination well 6s is specified, the well position determination unit 24 determines whether or not the position of the position determination well 6s is at a specified position (step S14). Measurement is started (step S15).

  On the other hand, if the position of the position determination well 6s is not at the specified position, the well position determination unit 24 issues a warning to the user and waits without starting measurement (step S16). The user recognizes from the warning that the installation direction of the reaction container 2 is wrong, and re-installs the well plate 2 (step S12). Thereafter, the well position specifying unit 20 specifies the position of the position determination well 6s (step S13), and the well position determination unit 24 determines whether the position of the position determination well 6s is at a specified position (step S13). S14) If it is at the specified position, the measurement is started (step S15).

2 reaction container 4 container 6 well 6s position determination well 8 fluorescent dye 9 reaction container installation unit 10 depression 12 measurement unit 14 optical sensor 16 guide rail 18 arithmetic processing unit 20 measurement value correction unit 22 well position specifying unit 24 well position determination unit 26 Correspondence Relationship Correction Unit 28 Sample Information Storage Unit 30 Display Unit

Claims (9)

A reaction vessel in which a plurality of wells for containing a specimen are provided on the same plane or on the same straight line,
A reaction container in which a specific well other than the center-symmetrical well among the plurality of wells is a position determination well in which a fluorescent dye is arranged. The reaction container is a plurality of wells arranged in a matrix in the plane,
The reaction container according to claim 1, wherein the position determination well is a well provided at or near a corner of the plane. A reaction vessel installation section for installing the reaction vessel according to claim 1 or 2,
A measurement unit for measuring the intensity of light of a wavelength to be measured from within each well provided in the reaction vessel installed in the reaction vessel installation unit, the position determination well of the reaction vessel And a measurement unit having a function of detecting fluorescence emitted from a fluorescent dye disposed therein.   When the wavelength of the fluorescence emitted from the fluorescent dye in the position determination well of the reaction container is the same as the wavelength of the measurement object, the baseline of the measurement value by the measurement unit for the position determination well of the reaction container And a measurement value correction unit configured to correct a measurement value of the well for position determination based on a difference between a measurement value and a baseline of a measurement value of the other well by the measurement unit. The fluorescence measuring device according to 1.   The fluorescence measuring apparatus according to claim 3, wherein the wavelength of fluorescence emitted from the fluorescent dye in the position determination well of the reaction container is different from the wavelength of the measurement target.   The well position specifying unit configured to specify the position of the well for position determination based on a measurement value of each well by the measuring unit, further comprising a well position specifying unit. The fluorescence measuring apparatus as described.   A well position determination unit configured to determine whether or not an actual position of the position determination well specified by the well position specifying unit matches a predetermined original position of the position determination well. The fluorescence measurement device according to claim 6, further comprising:   The fluorescence according to claim 7, wherein the well position determination unit is configured to issue a warning to a user when the actual position of the well position specifying unit and the original position do not match. measuring device. Specimen information storage unit for storing specimen information, which is information related to specimens accommodated in each well of each of the reaction containers installed in the reaction container installation unit, in association with the position of each well in which each specimen is accommodated When,
When the well position determining unit determines that the actual position of the well position specifying unit and the original position do not match, the actual position of the position determining well specified by the well position specifying unit Based on the well of the sample information in the sample information storage unit, the sample information stored in the sample information storage unit is associated with the position of the well in which each sample is actually stored. The fluorescence measurement apparatus according to claim 7, further comprising a correspondence correction unit that corrects the correspondence with the position of the position.

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