JP2019115373A - Method for producing rice flour bread that does not include gluten or thickener - Google Patents
Method for producing rice flour bread that does not include gluten or thickener Download PDFInfo
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- JP2019115373A JP2019115373A JP2019087269A JP2019087269A JP2019115373A JP 2019115373 A JP2019115373 A JP 2019115373A JP 2019087269 A JP2019087269 A JP 2019087269A JP 2019087269 A JP2019087269 A JP 2019087269A JP 2019115373 A JP2019115373 A JP 2019115373A
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Abstract
Description
本発明は、グルテン及び増粘剤を含まない米粉パンの製造方法に関する。 The present invention relates to a method for producing rice flour bread free of gluten and thickener.
近年、米粉の消費拡大を目的として米粉パンの開発が奨励されている。一方、小麦粉パンに含まれるグルテン(小麦グルテン)は小麦アレルギーやセリアック病症状等の原因となることが知られているため、小麦粉の代替品として米粉を使用した米粉パンはそれらの患者向け食品としても注目を集めている。 In recent years, development of rice flour bread has been encouraged for the purpose of expanding consumption of rice flour. On the other hand, since gluten (wheat gluten) contained in wheat flour bread is known to cause wheat allergy and celiac disease symptoms etc., rice flour bread using rice flour as a substitute for wheat flour is a food for those patients. Is also attracting attention.
グルテンは、ふっくらと膨らんだパンを製造する上で重要な成分である。一般的な小麦粉パンの製造においては、小麦粉に水を加えて練り合わせることにより、小麦粉に含まれるタンパク質グリアジンとグルテニンから粘り気と弾力性に富む「グルテン」が生成され、これがパン生地の伸張性及び弾力性を高めることで、発酵・焼成後の小麦粉パンが良好な膨らみや柔らかさを有するようになることが知られている。 Gluten is an important ingredient in making puffy bread. In general production of wheat flour bread, by adding water to the wheat flour and kneading, the protein gliadin and glutenin contained in the wheat flour produce "gluten" rich in tenacity and elasticity, which is the extensibility and elasticity of the dough. It is known that the flour bread after fermentation / baking comes to have good puffiness and softness by enhancing the property.
しかしグリアジンやグルテニンを含まない米粉のみを主原料として用いたパン生地では、生地をミキシングしてもグルテンが生成しない。このため一般的には、小麦粉パンと同じパン製造法では、穀物粉として米粉を100%使用したパンは十分に膨らまないとされている。そこで市販の米粉パンや米粉パン製造用の市販米粉のほとんどには、小麦粉やグルテンが配合されている。しかしそのような製品は小麦グルテンを比較的高量で含むため、小麦アレルギー患者やセリアック病患者は摂取できない。 However, bread dough using only rice flour containing no gliadin or glutenin as a main ingredient does not produce gluten even if the dough is mixed. For this reason, in general, in the same bread making method as wheat flour bread, bread using 100% of rice flour as grain flour is considered not to fully swell. Therefore, wheat flour and gluten are mixed in most of commercially available rice flour bread and commercially available rice flour for producing rice flour bread. However, such products contain relatively high amounts of wheat gluten and therefore can not be taken by wheat allergic patients or celiac disease patients.
グルテンを含まない米粉パンを製造する技術の開発も進められている。例えば、プラスチック発泡成形技術を利用した製パン方法(特許文献1)、α化米粉やα化澱粉を配合した生地を用いる方法(特許文献2及び3等)、増粘剤を添加する方法(特許文献4等)、などがある。しかし、これらの方法で製造した米粉パンは、小麦粉パンや、グルテン添加して製造した米粉パンに比べてふんわり感、柔らかさなどの点で品質が著しく劣るという問題がある。
Development of technology to produce gluten-free rice flour bread is also in progress. For example, a method of baking using a plastic foam molding technique (Patent Document 1), a method of using a dough compounded with pregelatinized rice flour or pregelatinized starch (
本発明者は、米粉にグルタチオンを配合することにより、膨らみが良くきめの細かい米粉パンを製造する方法を開発している(特許文献5)。さらに、米粉とソルガム粉を用いた米粉パンの製造方法も開発している(特許文献6)。しかしながら、グルタチオンやソルガム粉等の特別な副材料を使用せず、より簡便な方法でグルテン及び増粘剤を含まない米粉パンを製造できる方法の開発がなおも求められている。 The inventor of the present invention has developed a method for producing rice flour bread with a good expansion and fine texture by blending glutathione with rice flour (Patent Document 5). Furthermore, a method for producing rice flour bread using rice flour and sorghum flour has also been developed (Patent Document 6). However, there is still a need to develop a method which can produce rice flour bread free of gluten and thickener in a simpler method without using special auxiliary materials such as glutathione and sorghum flour.
本発明は、グルテン及び増粘剤を含まない膨らみの良い米粉パンの簡便な製造方法を提供することを課題とする。 An object of the present invention is to provide a simple method for producing a puffy rice flour bread free of gluten and thickener.
本発明者は、上記課題を解決するため鋭意検討を重ねた結果、所定の性状を示す米粉を使用して、グルテンや増粘剤等を使用することなく、膨らみの良い米粉パンを製造できることを見出し、本発明を完成するに至った。 As a result of intensive studies to solve the above problems, the present inventor can use rice flour exhibiting predetermined properties to produce rice flour bread with good swelling without using gluten, thickeners and the like. The present invention has been completed.
すなわち、本発明は以下を包含する。
[1]5%以下の損傷澱粉率及び80%以下の吸水率を示す米粉、水及び酵母を含み、かつグルテン及び増粘剤を含まないパン生地を、発酵させ、焼成することを含む、米粉パンの製造方法。
[2]米粉の損傷澱粉率が3%以上であり、吸水率が72%以上である、上記[1]に記載の方法。
[3]パン生地が、砂糖、食塩、及び油脂から選択される1つ以上をさらに含む、上記[1]又は[2]に記載の方法。
[4]前記パン生地を比容積4mL/g以上となるまで発酵させる、上記[1]〜[3]のいずれかに記載の方法。
[5]前記パン生地を調製する工程を含む、上記[1]〜[4]のいずれかに記載の方法。
[6]5%以下の損傷澱粉率及び80%以下の吸水率を示す米粉、水及び酵母を含み、かつグルテン及び増粘剤を含まないパン生地。
[7]米粉の損傷澱粉率が3%以上であり、吸水率が72%以上である、上記[6]に記載のパン生地。
[8]砂糖、食塩、及び油脂から選択される1つ以上をさらに含む、上記[6]又は[7]に記載のパン生地。
[9]発酵させた、上記[6]〜[8]のいずれかに記載のパン生地。
[10]比容積が4mL/g以上である、上記[9]に記載のパン生地。
[11]上記[6]〜[10]のいずれか記載のパン生地を用いて製造された、グルテン及び増粘剤を含まない米粉パン。
That is, the present invention includes the following.
[1] A rice flour bread comprising fermenting and baking a bread dough comprising rice flour, water and yeast exhibiting a damaged starch ratio of 5% or less and a water absorption coefficient of 80% or less and containing no gluten and no thickener. Manufacturing method.
[2] The method according to the above [1], wherein the damaged starch rate of rice flour is 3% or more and the water absorption rate is 72% or more.
[3] The method according to the above [1] or [2], wherein the bread dough further comprises one or more selected from sugar, salt and fats and oils.
[4] The method according to any one of the above [1] to [3], wherein the bread dough is fermented to a specific volume of 4 mL / g or more.
[5] The method according to any one of the above [1] to [4], which comprises the step of preparing the bread dough.
[6] A bread dough comprising rice flour, water and yeast showing a damaged starch ratio of 5% or less and a water absorption of 80% or less, and containing no gluten and no thickener.
[7] The bread dough according to the above [6], wherein the damaged starch rate of rice flour is 3% or more and the water absorption rate is 72% or more.
[8] The bread dough according to the above [6] or [7], further comprising one or more selected from sugar, salt, and fats and oils.
[9] The bread dough according to any one of the above [6] to [8] which is fermented.
[10] The dough according to the above [9], which has a specific volume of 4 mL / g or more.
[11] A gluten-free and thickener-free rice flour bread produced using the dough according to any one of the above [6] to [10].
本発明によれば、グルテン及び増粘剤を含まない膨らみの良い米粉パンを簡便に製造することができる。 According to the present invention, a glutinous rice flour bread free from gluten and thickener can be easily produced.
以下、本発明を詳細に説明する。
本発明は、所定の性状の米粉を使用して、グルテン構成タンパク質やそれを含む穀物粉(小麦粉など)、グルテン、増粘剤等を使用することなく、米粉パンを製造する方法に関する。
Hereinafter, the present invention will be described in detail.
The present invention relates to a method for producing rice flour bread using rice flour of a predetermined property without using gluten-constituting proteins and cereal flour (such as wheat flour) containing the same, gluten, thickeners and the like.
本発明では、パンの主原料となる穀物粉として米粉を使用する。米粉とは、イネ(Oryza sativa)の種子(生米)を粉砕して得られた粉末をいう。米粉の原料となる米は、限定するものではないが、うるち米が好ましい。うるち米は、例えば、ジャポニカ米、インディカ米、又はジャバニカ米であってよく、さらに、それらに属する様々な品種の米であってよい。米の品種としては、以下に限定するものではないが、例えば、タカナリ、ミズホチカラ、ゆめふわりなどの、米粉用米として好適とされる品種であってもよいし、アミロース含有率が高い、中程度、又は低い品種などであってもよい。米粉はまた、様々な品種の米粉の混合物であってもよい。なお、本発明で原料として用いる米粉は、米を加熱によりα化(糊化)した後に乾燥及び粉末化して得られるα化米粉を含む必要はない。 In the present invention, rice flour is used as grain flour which is a main ingredient of bread. Rice flour refers to a powder obtained by grinding rice (Oryza sativa) seeds (raw rice). Although rice used as a raw material of rice flour is not limited, it is preferable to use light rice. The perishable rice may be, for example, Japonica rice, Indica rice, or Javanese rice, and may further be various varieties of rice belonging to them. The variety of rice is not limited to the following, but may be, for example, varieties suitable for rice flour, such as Takanari, Mizuhochikara, Yumewari, etc., and may have a high amylose content, which is moderate Or a low variety or the like. The rice flour may also be a mixture of various varieties of rice flour. In addition, the rice flour used as a raw material by this invention does not need to contain the gelatinization rice flour obtained by drying and pulverizing after gelatinizing (gelatinizing) rice by heating.
本発明では、5%以下の損傷澱粉率及び80%以下の吸水率を示す米粉を使用する。好適な一実施形態では、米粉の損傷澱粉率が3%以上、例えば4%以上であることも好ましい。好適な一実施形態では、吸水率が72%以上、例えば74%以上であることも好ましい。以下に限定するものではないが、好ましい一実施形態では、米粉の損傷澱粉率は3%〜5%であり、吸水率は72%〜80%である。 In the present invention, rice flour is used which exhibits a damaged starch ratio of 5% or less and a water absorption of 80% or less. In a preferred embodiment, it is also preferred that the damaged starch rate of rice flour is 3% or more, for example 4% or more. In a preferred embodiment, it is also preferred that the water absorption be 72% or more, for example 74% or more. Although not limited to the following, in a preferred embodiment, the damaged starch rate of rice flour is 3% to 5%, and the water absorption rate is 72% to 80%.
米粉の損傷澱粉率は常法により測定することができる。本発明では、特に、損傷澱粉測定キットStarch Damage Assay Kit(Megazyme社)又はそれと同等のキット若しくは試薬を使用して決定される損傷澱粉率に基づいて、米粉の性状を判定することができる。具体的には、後述の実施例に記載するように、まず、米粉100mgとアミラーゼ溶液(50U/ml)を別々の容器で40℃で約5分、プレインキュベーションした後、米粉に1mLのアミラーゼ溶液を加え、ボルテックスミキサーで混合し、40℃で10分間反応させる。次いで硫酸溶液(0.2%v/v)を8ml添加し、ボルテックスミキサーで混合して反応を停止させ、3,000rpmで5分遠心する。上清の0.1mlを採取し、0.1mlのアミログルコシダーゼ溶液を加えて40℃で10分間反応させた後、4mlのグルコース測定試薬GOPOD(グルコースオキシダーゼ及びペロキシダーゼ)溶液を加え、40℃で20分間反応させる。さらに反応液について510nmで吸光度を測定する。150mg/mlのグルコース標準液及び緩衝液(ブランク)についてGOPOD溶液を加えて反応させ、吸光度の測定を行い、検量線を作成する。この検量線に基づいて米粉の損傷澱粉率(%)を決定することができる。 The damaged starch rate of rice flour can be measured by a conventional method. In the present invention, it is possible to determine the quality of rice flour based on, in particular, the rate of damaged starch determined using a damaged starch measurement kit Starch Damage Assay Kit (Megazyme) or an equivalent kit or reagent. Specifically, as described in the examples below, first, 100 mg of rice flour and amylase solution (50 U / ml) are preincubated in separate containers at 40 ° C. for about 5 minutes, and then 1 mL of amylase solution in rice flour Is added, mixed with a vortex mixer, and reacted at 40 ° C. for 10 minutes. Then add 8 ml of sulfuric acid solution (0.2% v / v), mix by vortex mixer to stop the reaction, and centrifuge at 3,000 rpm for 5 minutes. After taking 0.1 ml of the supernatant, adding 0.1 ml of amyloglucosidase solution and reacting at 40 ° C. for 10 minutes, add 4 ml of glucose measuring reagent GOPOD (glucose oxidase and peroxidase) solution and carry out at 40 ° C. Let react for 20 minutes. Further, the absorbance of the reaction solution is measured at 510 nm. A GOPOD solution is added to a 150 mg / ml glucose standard solution and buffer solution (blank) to make a reaction, absorbance is measured, and a calibration curve is prepared. Based on this calibration curve, the percent damaged starch of rice flour can be determined.
米粉の吸水率は常法により測定することができる。本発明では、特に、後述の実施例に記載するようにして測定した吸水率に基づいて、米粉の性状を判定することができる。具体的には、米粉(乾燥状態)10gを50mLチューブに入れ、50mLの目盛までイオン交換水を添加した後、室温で一晩放置し、3000rpmで30分間遠心した後、上清を捨て、吸水後の米粉重量(米粉と米粉に吸収された水の合計重量)を測定する。測定値に基づいて、米粉の吸水率を以下の式で算出することができる。
吸水率(%)=(吸水後の米粉重量−吸水前の米粉重量(10g))/吸水前の米粉重量(10g)×100
The water absorption rate of rice flour can be measured by a conventional method. In the present invention, in particular, the properties of rice flour can be determined based on the water absorptivity measured as described in the following examples. Specifically, 10 g of rice flour (dry state) is placed in a 50 mL tube, ion-exchanged water is added to a scale of 50 mL, and left overnight at room temperature, centrifuged at 3000 rpm for 30 minutes, and then the supernatant is discarded. Measure the weight of rice flour after (total weight of rice flour and water absorbed by rice flour). Based on the measured values, the water absorption rate of rice flour can be calculated by the following equation.
Water absorption rate (%) = (weight of rice flour after water absorption-weight of rice flour before water absorption (10 g)) / weight of rice flour before water absorption (10 g) × 100
本発明で用いる米粉は、例えばきめの整ったパンを製造する目的では、好ましくは200μm以下、より好ましくは70〜130μmの最頻粒径を示すものであってもよい。米粉について、粒度分布測定装置(LS13320[ベックマンコールター]又はそれと同等の装置)を使用し、マニュアルに従って乾式法による粒度分布の計測を行い、その粒度分布計測値から米粉の最頻粒径を求めることができる。 The rice flour used in the present invention may have a mode diameter of preferably 200 μm or less, more preferably 70 to 130 μm, for the purpose of producing a well-defined bread, for example. For rice flour, use a particle size distribution analyzer (LS13320 [Beckman Coulter] or equivalent device), measure the particle size distribution according to the dry method according to the manual, and determine the most frequent particle size of rice flour from the particle size distribution measurement value. Can.
本発明で用いる上記の米粉は、限定するものではないが、湿式気流粉砕法により製造することが好ましい。上記の米粉は市販品として入手することもできる。 Although the above-mentioned rice flour used by this invention is not limited, It is preferable to manufacture by a wet air flow grinding method. The above-mentioned rice flour can also be obtained as a commercial product.
本発明では、上記の米粉、水及び酵母を含み、かつグルテン及び増粘剤を含まないパン生地を用いて米粉パンを製造する。パン生地中の水の量は、米粉を用いたパンの製造に原料として使用する水の一般的な量であってよいが、例えば、米粉の量の70〜120重量%であってよい。パン生地中の水は、水として添加したものであってよいが、他の原料の一成分として添加したものであってもよい。例えば、パン生地中の水は、パン生地に添加した牛乳、豆乳、ジュースなどの飲料等に由来する水分であってもよい。常温の水又は飲料等を原料として米粉に添加してもよいし、加温又は冷却した水又は飲料等を加えてもよい。 In the present invention, rice flour bread is produced using bread dough containing the above-mentioned rice flour, water and yeast and containing no gluten and no thickener. The amount of water in the dough may be a common amount of water used as a raw material in the manufacture of bread using rice flour but may for example be 70 to 120% by weight of the amount of rice flour. Water in the bread dough may be added as water, but may be added as one component of another raw material. For example, the water in the dough may be water derived from milk, soy milk, beverages such as juice, etc. added to the dough. You may add to rice flour by using as a raw material water, a drink, etc. of normal temperature, and you may add the water or drink etc. which were heated or cooled.
パン生地に配合する酵母は、パンの発酵に使用できる任意の酵母であってよいが、典型的には、パン酵母(パン製造に使用されるサッカロミセス・セレビシエ(Saccharomyces cerevisiae)株)である。本発明で用いる酵母は、グルテン構成タンパク質を分泌しない。酵母は、乾燥イーストであっても生イーストであってもよい。また酵母は、いわゆる発酵種(スターター)に含まれるものであってもよい。発酵種は、自然界に存在する酵母を発酵・増殖させて得られる発酵物であり、パン酵母以外に乳酸菌等の多様な微生物を含み得る。そのような発酵種を酵母の供給源として、パン生地に混合してもよい。但しグルテン及び増粘剤を含まないパン生地を調製するために、発酵種の調製もグルテン及び増粘剤を含まない原料を用いて行う必要がある。当業者であれば、パン製造に適した酵母の配合量は適宜調節することができるが、一般的には、乾燥重量で米粉の量の0.5〜5質量%となる量の酵母を用いればよい。酵母は、例えば、最初から米粉と共にミキシングしてもよいし、一旦ミキシングした後の生地に添加してさらにミキシングしてもよい。予備発酵を行った後の酵母を米粉又はパン生地に添加してもよいし、予備発酵を行うことなく酵母を米粉又はパン生地に添加してもよい。 The yeast incorporated in the bread dough may be any yeast that can be used for fermentation of bread but is typically baker's yeast (Saccharomyces cerevisiae strain used in bread manufacture). The yeast used in the present invention does not secrete gluten-constituting proteins. The yeast may be dry yeast or live yeast. Moreover, yeast may be contained in so-called fermented species (starter). The fermented species is a fermented product obtained by fermenting and growing yeast existing in the natural world, and may contain various microorganisms such as lactic acid bacteria in addition to baker's yeast. Such fermented species may be mixed into bread dough as a source of yeast. However, in order to prepare gluten and thickener-free bread dough, it is necessary to prepare fermented species using gluten- and thickener-free raw materials. A person skilled in the art can appropriately adjust the blending amount of yeast suitable for bread production, but generally, an amount of 0.5 to 5% by mass of the amount of rice flour based on dry weight is used. Just do it. The yeast may be mixed with rice flour from the beginning, for example, or may be added to the dough once mixed and further mixed. The yeast after pre-fermentation may be added to rice flour or bread dough, or the yeast may be added to rice flour or dough without pre-fermentation.
本発明に係るパン生地は、グルテン及び増粘剤を含まない限り、他の原料を含んでもよい。本発明に係るパン生地は、例えば、砂糖などの糖類、食塩、及び油脂から選択される1つ以上をさらに含んでもよい。油脂は、オリーブ油などの植物油脂、バターなどの動物油脂、ショートニング、マーガリン等の任意の油脂又はそれらの混合物であってよいが、好ましい例としてバターが挙げられる。 The bread dough according to the present invention may contain other ingredients as long as it does not contain gluten and a thickener. The bread dough according to the present invention may further include, for example, one or more selected from sugars such as sugar, sodium chloride, and fats and oils. The fats and oils may be vegetable fats and oils such as olive oil, animal fats and oils such as butter, shortenings, optional fats and oils such as margarine, or mixtures thereof, but butter is a preferable example.
本発明において「食塩」とは、食品グレード又は医薬品グレードの塩(塩化ナトリウムを主成分とする)を意味するものとする。食塩は主成分の塩化ナトリウムに加えて、例えば、塩化カリウム、塩化カルシウム、塩化マグネシウム等の他の無機塩類を含んでいてもよい。任意の食塩製品を、本発明に係るパン生地に配合する食塩として用いることができる。例えば、市販の精製塩などの高純度塩(ほぼ塩化ナトリウムのみを含有する)や、未精製塩(塩化ナトリウムを主成分とするが、他の無機塩類をより多い量で含有する)などを用いることができる。精製塩(乾燥重量で99%以上の塩化ナトリウムを含有する)をパン生地に添加する場合、精製塩の量はその塩化ナトリウム含量とほぼ等しいことから、パン生地における食塩の含有量は、精製塩の添加量に基づいて計算することができる。一方、未精製塩(乾燥重量で99%未満の塩化ナトリウムを含む)をパン生地に添加する場合、パン生地における食塩含有量は、添加した未精製塩のナトリウム含量から算出される塩化ナトリウム相当量に基づいて計算する。なお塩化ナトリウム相当量は、ナトリウム含量に2.54を乗算することによって算出される。 In the present invention, “salt” means food grade or pharmaceutical grade salt (based on sodium chloride). In addition to sodium chloride as the main component, sodium chloride may contain, for example, other inorganic salts such as potassium chloride, calcium chloride, magnesium chloride and the like. Any salt product can be used as the salt to be blended into the bread dough according to the present invention. For example, high purity salts such as commercially available purified salts (containing almost only sodium chloride), unpurified salts (containing sodium chloride as a main component but containing other inorganic salts in a larger amount), etc. are used be able to. When a purified salt (containing 99% or more by dry weight sodium chloride) is added to bread dough, the amount of purified salt is approximately equal to its sodium chloride content, so the salt content in the bread dough is equivalent to the addition of the purified salt It can be calculated based on the quantity. On the other hand, when unrefined salt (containing less than 99% by dry weight sodium chloride) is added to the dough, the salt content in the dough is based on the equivalent sodium chloride calculated from the sodium content of the added unrefined salt Calculate. The sodium chloride equivalent is calculated by multiplying the sodium content by 2.54.
本発明において砂糖としては、白砂糖、黒砂糖、キビ砂糖、ショ糖等の任意の砂糖を用いることができる。また、他の糖類(ぶどう糖、果糖、蜂蜜、麦芽糖、黒糖、グラニュー糖等;但し多糖類を除く)を用いることもできる。 In the present invention, as sugar, any sugar such as white sugar, black sugar, millet sugar, sucrose and the like can be used. In addition, other sugars (glucose, fructose, honey, maltose, brown sugar, granulated sugar, etc .; except polysaccharides) can also be used.
本発明に係るパン生地はまた、肉・魚類、ナッツ類、きのこ類、果実、野菜、ハーブ、豆類、飲料、乳製品、発酵食品、甘味料、菓子、香辛料等の食品又は食品原料、例えば、卵、ハム、ソーセージ、ベーコン、アーモンド、ニンジン、ゴマ、レーズン、リンゴ、枝豆、野菜ジュース、ローズマリー、牛乳、チーズ、ヨーグルト、トレハロース、チョコレート、シナモンパウダーなどを含んでもよい。本発明に係るパン生地はまた、グルテン及び増粘剤を含まない限り、乳化剤、保存料、香料、着色料等の食品添加物を含んでもよい。 The bread dough according to the present invention is also a food or food material such as meat / fish, nuts, mushrooms, fruits, vegetables, herbs, beans, beverages, dairy products, fermented foods, sweeteners, sweets, spices and the like, for example, eggs Ham, sausage, bacon, almond, carrot, sesame, raisin, apple, edamame, vegetable juice, rosemary, milk, cheese, yoghurt, trehalose, chocolate, cinnamon powder, etc. may be included. The bread dough according to the present invention may also contain food additives such as emulsifiers, preservatives, flavors and colorants, as long as it does not contain gluten and thickeners.
本発明に係るパン生地は、グルテン構成タンパク質や、グルテン構成タンパク質を含む穀物粉(小麦粉、ライムギ粉、大麦粉、オーツ麦粉、又はそれらの交配雑種の穀物粉等)を含まない。本発明に係るパン生地は、穀物粉としては米粉のみを含むことがさらに好ましく、5%以下の損傷澱粉率及び80%以下の吸水率を示す米粉のみを含むことが特に好ましい。本発明に係るパン生地はまた、グルテン、グルテン構成タンパク質、及びグルテン構成タンパク質を含む穀物粉(小麦粉、ライムギ粉、大麦粉、オーツ麦粉、又はそれらの交配雑種の穀物粉等)のいずれも原料として使用せずに作製されることから、グルテンを含まない。ここで、パン生地がこれらの成分を「含まない」とは、パン生地中にこれらの成分が有効量で存在しないことを意味する。グルテン、グルテン構成タンパク質、又は他の穀物粉が、生地の原料としては使用されていないにもかかわらず、パン生地又はパン製造工程においてごく微量に混入した場合は、そのパン生地中には有効量のグルテン、グルテン構成タンパク質、又は他の穀物粉が存在しないため、当該パン生地はそれらを含まないものと判定される。より具体的には、パン生地中のグルテンの混入量が、米粉の量の0.5重量%未満(好ましくは0.1重量%未満)である場合、そのパン生地中には有効量のグルテンは存在せず、本発明に係るそのようなパン生地はグルテンを含まないものとする。また、パン生地中のグルテン構成タンパク質又は他の穀物粉の混入量が、米粉の量の1.0重量%未満(好ましくは0.1重量%未満)である場合、そのパン生地中には有効量のグルテン構成タンパク質又は他の穀物粉が存在せず、本発明に係るそのようなパン生地はグルテン構成タンパク質又は他の穀物粉を含まないものとする。 The bread dough according to the present invention does not contain gluten-constituting proteins or cereal flours containing gluten-constituting proteins (wheat flour, rye flour, barley flour, oat flour, cereal flour of hybrid hybrids thereof, etc.). The bread dough according to the present invention further preferably contains only rice flour as grain flour, and particularly preferably contains only rice flour showing a damaged starch ratio of 5% or less and a water absorption rate of 80% or less. The bread dough according to the present invention also uses any of gluten, gluten-constituting protein, and cereal flour containing gluten-constituting protein (wheat flour, rye flour, barley flour, oat flour, cereal flour of hybrid hybrids thereof, etc.) Contains no gluten because it is made without Here, the bread dough "does not contain" these ingredients means that these ingredients are not present in the bread dough in effective amounts. If gluten, gluten-constituting proteins, or other cereal flours are not used as ingredients for dough, but are incorporated in trace amounts in the bread dough or bread making process, an effective amount of gluten in the dough Because no gluten-constituting proteins or other flours are present, the bread dough is judged to be free of them. More specifically, when the amount of gluten incorporation in bread dough is less than 0.5% by weight (preferably less than 0.1% by weight) of the amount of rice flour, an effective amount of gluten is present in the dough. Without this, such bread dough according to the present invention is gluten free. Also, if the amount of gluten-constituting protein or other cereal flour incorporated in the dough is less than 1.0% by weight (preferably less than 0.1% by weight) of the amount of rice flour, an effective amount of it is included in the dough. In the absence of gluten-constituting proteins or other cereal flours, such bread dough according to the present invention shall be free of gluten-constituting proteins or other cereal flours.
好ましい実施形態では、本発明に係るパン生地は、より厳密に、「グルテンフリー」であってもよい。本発明において「グルテン」は、小麦粉由来の小麦グルテンの他、他の穀物粉由来のグルテン様タンパク質も包含する。グルテンは、穀物粉に含まれるグルテン構成タンパク質(小麦粉の場合、グリアジンとグルテニン)が水の存在下で反応することで生じる。小麦のグリアジンに対応するグルテン構成タンパク質は、大麦ではホルダイン、ライ麦ではセカリン、オーツ麦ではアベニンと呼ばれる。本発明において「グルテンフリー」とは、グルテンの含有量が当該食品の総重量(総質量)の0.002%以下(20mg/kg以下)であることを意味する。これは2008年にコーデックス(Codex)委員会が「グルテンフリー」の基準として設定した値である。典型的には、グルテンフリー製品専用の製造ラインを用いて本発明に係るパン生地等を製造することにより、小麦粉やグルテン等の混入を防止し、グルテンフリーのパン生地を製造することができる。 In a preferred embodiment, the bread dough according to the invention may be more strictly "gluten free". In the present invention, “gluten” includes wheat gluten derived from wheat flour and gluten-like proteins derived from other cereal flours. Gluten is produced by the reaction of gluten-constituting proteins (gliadin and glutenin in the case of wheat flour) contained in cereal flour in the presence of water. The gluten-constituting protein corresponding to gliadin in wheat is called hordein in barley, secalin in rye, and avenin in oats. In the present invention, “gluten free” means that the gluten content is 0.002% or less (20 mg / kg or less) of the total weight (total weight) of the food. This is the value set by the Codex committee in 2008 as a "gluten free" standard. Typically, by manufacturing the bread dough and the like according to the present invention using a manufacturing line dedicated to gluten-free products, it is possible to prevent mixing of flour, gluten and the like and to manufacture gluten-free bread dough.
グルテンを含まない米粉パンの製造においては通常、増粘剤が使用されるが、本発明に係るパン生地は、グルテンだけでなく、増粘剤も含まない。増粘剤としては、食品分野で使用できる多種多様な増粘剤、例えば増粘多糖類、ゲル化剤等が挙げられる。増粘剤の例としては、アルギン酸、アラビアガム、カラギナン、カルボキシメチルセルロース、グアーガム、キサンタンガム、ローカストビーンガム、ペクチン、セルロース、タマリンドシードガム、サイリウムシードガム、グルコマンナン、ゼラチン、寒天、大豆多糖類、タピオカ澱粉、コーンスターチ、葛澱粉、片栗粉、ばれいしょ澱粉並びにそれらの誘導体(アセチル化物、エステル化物、分解物など)があるが、これらに限定されない。なお本発明の方法のパン生地又はパン製造中に、米粉、酵母又は他の原料から増粘作用のある成分が生成したとしても、そのような成分は「増粘剤」には含めないものとする。ここで、本発明に係るパン生地が増粘剤を「含まない」とは、パン生地中に有効量の増粘剤が存在しないことを意味する。増粘剤が生地の原料としては使用されていないにもかかわらず、パン生地又はパン製造工程においてごく微量に混入した場合は、そのパン生地中には有効量の増粘剤が存在しないため、本発明に係るそのようなパン生地は増粘剤を含まないものと判定される。より具体的には、パン生地中の増粘剤の混入量が、米粉の量の0.05重量%未満(好ましくは0.005重量%未満)である場合、そのパン生地中には有効量の増粘剤が存在せず、本発明に係るそのようなパン生地は増粘剤を含まないものとする。 Although thickeners are usually used in the production of gluten-free rice flour bread, the bread dough according to the present invention contains not only gluten but also thickeners. Thickeners include a wide variety of thickeners that can be used in the food field, such as thickening polysaccharides, gelling agents, and the like. Examples of thickeners include alginic acid, gum arabic, carrageenan, carboxymethylcellulose, guar gum, xanthan gum, locust bean gum, pectin, cellulose, tamarind seed gum, psyllium seed gum, glucomannan, gelatin, agar, soy polysaccharides, tapioca There are, but not limited to, starch, corn starch, mulberry starch, corn starch, potato starch and derivatives thereof (acetylated products, esterified products, degradation products, etc.). In addition, even if ingredients having a thickening action are produced from rice flour, yeast or other raw materials during bread dough or bread production of the method of the present invention, such ingredients shall not be included in the "thickener". . Here, the bread dough according to the present invention "contains no thickener" means that no effective amount of thickener is present in the bread dough. The present invention does not use thickener as a raw material of dough, but when it is mixed in a very small amount in bread dough or bread making process, no effective amount of thickener is present in the dough, so the present invention Such bread dough according to is determined to be free of thickener. More specifically, when the amount of thickener mixed in the dough is less than 0.05% by weight (preferably less than 0.005% by weight) of the amount of rice flour, the effective amount is increased in the dough. There is no viscosity, and such bread dough according to the invention is free of thickener.
なお本発明において、グルテン、グルテン構成タンパク質、米粉以外の穀物粉、又は増粘剤を「含まない」ものとする上記基準は、パン生地だけでなく、米粉パンについても同様に適用される。 In the present invention, gluten, gluten-constituting protein, cereal flour other than rice flour, or the above-mentioned standard that "contains no thickener" applies not only to bread dough but also to rice flour bread.
本発明に係るパン生地は、上記のようなパン原料から常法により調製することができ、具体的には、米粉、水、酵母、及び他の原料を1以上の工程によりミキシングすることにより調製することができる。食品加工分野において「ミキシング」とは、粉体及び液体を含む原料混合物をその成分を均一に分散させるように練ることをいう。本発明においてミキシングは、例えばミキシング装置やホームベーカリー等の攪拌機能により行うこともできるし、手で行うこともできる。ミキシングは、製パンにおいてミキシングに用いられる通常の温度で行えばよく、特に制限するものではないが、一般的には室温程度、例えば4℃〜32℃程度、好ましくは15〜30℃で行えばよい。ミキシングは当業者に公知の通常のミキシング時間、例えばミキシング工程1回につき3分〜40分程度で行うことができるが、好ましくはミキシング工程1回につき10〜30分程度行えばよい。ミキシングは1回でもよいし、複数回行ってもよい。 The bread dough according to the present invention can be prepared from bread ingredients as described above by a conventional method, and specifically, it is prepared by mixing rice flour, water, yeast, and other ingredients in one or more steps. be able to. In the food processing field, "mixing" refers to kneading of a raw material mixture containing powder and liquid so as to uniformly disperse its components. In the present invention, the mixing can be performed by, for example, a stirring device such as a mixing device or a home bakery, or can be performed by hand. The mixing may be performed at a usual temperature used for mixing in bread making, and is not particularly limited, but generally it is performed at about room temperature, for example, about 4 ° C. to 32 ° C., preferably 15 to 30 ° C. Good. The mixing can be performed in a conventional mixing time known to those skilled in the art, for example, about 3 minutes to 40 minutes per mixing step, but preferably about 10 to 30 minutes per mixing step. The mixing may be performed once or plural times.
本発明に係るパン生地は、米粉、水、酵母、及び必要に応じて他の原料を1つに混ぜ合わせ、それを1工程でミキシングすることを含む方法により調製してもよい。あるいは本発明に係るパン生地は、2以上のミキシング工程を含む方法で調製することもできる。一実施形態では、例えば、米粉、水、及び必要に応じて他の1以上の原料を1つに混ぜ合わせ、ミキシングした後、酵母、砂糖などの糖類、及び必要に応じて他の1以上の原料をさらに添加し、ミキシングすることにより、本発明に係るパン生地を調製することもできる。最終的なミキシング後に、他の原料(例えば、ナッツ類、チーズ、果実など)をさらに添加(例えば混合、包み込み、トッピング又は埋め込み)してもよい。米粉と水のミキシングの後、一定時間放置して十分な浸漬時間を置いてから、酵母等の他の原料を添加してもよい。 The bread dough according to the present invention may be prepared by a method including mixing rice flour, water, yeast and, if necessary, other ingredients into one, and mixing them in one step. Alternatively, the bread dough according to the present invention can be prepared by a method including two or more mixing steps. In one embodiment, for example, after mixing and mixing rice flour, water, and optionally one or more other ingredients into one, yeast, saccharides such as sugar, and optionally one or more other The bread dough according to the present invention can also be prepared by further adding and mixing the raw materials. After final mixing, other ingredients (eg, nuts, cheese, fruits, etc.) may be further added (eg, mixed, wrapped, topped or embedded). After mixing the rice flour and water, it may be left for a given time to allow a sufficient immersion time, and then other raw materials such as yeast may be added.
上記のようにして調製したパン生地は、必要に応じて容器に入れたり、分割又は成形したりした後、パン製造に用いられる任意の方法で発酵させることができる。本発明に係るパン生地は、通常は、例えば、発酵に適した温度(4〜50℃、通常は25〜45℃)下に通常は20分〜4時間程度(より一般的には25分〜2時間程度)置くことにより、発酵させることができる。発酵は、1回の発酵工程のみで行ってもよいし、2回以上の発酵工程で行ってもよい。例えばパン生地について、好ましくは比較的低温(例えば15〜32℃)で15分〜2時間程度(より一般的には30分〜90分程度)置くことにより発酵させる一次発酵(フロアタイムとも呼ぶ)を行ってもよい。一次発酵によりある程度発酵が進んだ段階で、さらに4℃〜45℃(より好ましくは25〜42℃)で通常は15分〜2時間程度(より一般的には30分〜90分程度)にわたりさらに発酵させてもよい(ホイロと呼ばれる最終発酵)。本発明に係るパン生地は、ミキシング後、最初に生地をパン成形用の容器(型)に入れて発酵工程を開始することにより、最終発酵の完了まで中断なしで発酵工程を行ってもよい。 The bread dough prepared as described above may be fermented by any method used for bread production, after being put in a container, divided or shaped as needed. The bread dough according to the present invention is usually, for example, usually about 20 minutes to 4 hours (more usually 25 minutes to 2 hours) under a temperature suitable for fermentation (4 to 50 ° C., usually 25 to 45 ° C.) It can be fermented by putting it for about time. Fermentation may be performed in only one fermentation step, or may be performed in two or more fermentation steps. For example, with regard to bread dough, primary fermentation (also referred to as floor time) which is preferably fermented by placing at relatively low temperature (for example, 15 to 32 ° C.) for about 15 minutes to 2 hours (more generally, about 30 minutes to about 90 minutes) You may go. At the stage where the fermentation has progressed to some extent by the primary fermentation, it is usually further added at 4 ° C. to 45 ° C. (more preferably 25 to 42 ° C.) for usually 15 minutes to 2 hours (more generally 30 minutes to 90 minutes) May be fermented (final fermentation called hiruo). The bread dough according to the present invention may be subjected to the fermentation process without interruption until completion of the final fermentation by first placing the dough in a pan-forming container (type) after mixing and starting the fermentation process.
本発明では、本発明に係るパン生地を発酵段階で顕著に膨らませることができる。本発明では、好ましい実施形態では、発酵段階終了時にパン生地の比容積が3mL/g以上、特に4mL/g以上となり得る。本発明の方法では、本発明に係るパン生地を比容積3mL/g以上、特に4mL/g以上となるまで発酵させることが好ましい。ここで、パン生地の比容積は、発酵させたパン生地の容積(mL)を、パン生地の重量で除算することにより算出することができる。 In the present invention, the bread dough according to the present invention can be significantly expanded at the fermentation stage. According to the invention, in a preferred embodiment, the specific volume of the dough at the end of the fermentation stage can be 3 mL / g or more, in particular 4 mL / g or more. In the method of the present invention, it is preferable to ferment the bread dough according to the present invention to a specific volume of 3 mL / g or more, particularly 4 mL / g or more. Here, the specific volume of the dough can be calculated by dividing the volume (mL) of the fermented dough by the weight of the dough.
上記のようにして調製した本発明に係るパン生地は、任意の方法で、例えば密封容器又は密封袋などにパッケージングしてもよい。本発明に係るパン生地について、例えば殺菌、冷蔵、冷凍等の処理を行ってもよい。 The dough according to the invention prepared as described above may be packaged in any way, for example in a sealed container or a sealed bag. The bread dough according to the present invention may be subjected to treatments such as sterilization, refrigeration, freezing and the like.
本発明は上記のようなパン生地の調製(製造)方法を提供する。本発明に係るパン生地の調製方法は、ミキシング工程に加えて、場合により分割工程、成形工程、1回以上の発酵工程、パッケージング工程、殺菌工程、及び冷蔵又は冷凍工程等の1つ以上の任意の工程を含んでもよい。 The present invention provides a method of preparing (producing) bread dough as described above. The bread dough preparation method according to the present invention may optionally include one or more optional steps such as a dividing step, a forming step, one or more fermentation steps, a packaging step, a sterilization step, and a refrigeration or freezing step, in addition to the mixing step. May be included.
本発明はまた、上記のような方法で調製した本発明に係るパン生地も提供する。本発明に係るパン生地は、焼成完了前のものを指す。本発明に係るパン生地はまた、例えば殺菌、冷蔵、又は冷凍等の処理を行ったパン生地も包含する。本発明に係るパン生地は、レトルトパウチ製品であってもよい。本発明に係るパン生地はまた、部分焼成されているが焼成が完了していない生地も包含する。本発明に係るパン生地は、焼成前である限り、ミキシング等により調製された生地を容器に入れたり、成形したり、生地に他の食材を混ぜたり包み込んだり載せたり埋め込んだりしたものも包含する。 The present invention also provides a bread dough according to the present invention prepared by the method as described above. The bread dough according to the present invention refers to those before baking is completed. The bread dough according to the present invention also includes bread dough which has been subjected to treatments such as sterilization, refrigeration or freezing. The bread dough according to the present invention may be a retort pouch product. The bread dough according to the invention also includes dough which has been partially baked but which has not yet been baked. The bread dough according to the present invention includes those obtained by mixing the dough prepared by mixing or the like into a container, forming the dough, mixing, wrapping, placing or embedding other food in the dough as long as it is before baking.
本発明に係るパン生地を、発酵させ、さらに焼成(ベーキング)することにより、米粉パンを製造することができる。生地の焼成は、常法により行うことができる。具体的には、生地をオーブン、電子レンジ、釜等の任意の手段で加熱(例えば100℃〜240℃での加熱)することにより焼成工程を実施すればよい。焼成時間は、一般的には5分〜100分程度である。焼成温度や焼成時間は、当業者であれば適宜調節することができる。本発明に係るパン生地は、焼成後も良好な膨らみを示す。本発明に係る米粉パンは、焼成後の比容積が3mL/g以上、特に4mL/g以上であることが好ましい。ここで、パンの比容積(パン比容積)は、焼成後のパンの容積(mL)を、パンの重量で除算することにより算出することができる。 A rice flour bread can be manufactured by fermenting the bread dough which concerns on this invention, and also baking (baking). The baking of the dough can be carried out by a conventional method. Specifically, the baking step may be performed by heating (for example, heating at 100 ° C. to 240 ° C.) the dough by any means such as an oven, a microwave oven, or a kettle. The firing time is generally about 5 minutes to 100 minutes. The baking temperature and the baking time can be appropriately adjusted by those skilled in the art. The bread dough according to the present invention exhibits good swelling even after baking. The rice flour bread according to the present invention preferably has a specific volume after baking of 3 mL / g or more, particularly 4 mL / g or more. Here, the specific volume of the bread (bread specific volume) can be calculated by dividing the volume (mL) of the baked bread by the weight of the bread.
焼き上がったパンに、さらにナッツ類、果実、クリーム、シロップ等をトッピングしたり、惣菜やハム等を挟んだりして、菓子パンや調理パン等を製造することもできる。このようにして加工されたパンも本発明に係るパンの範囲に含まれる。本発明に係る米粉パンは、食パン、ロールパン、丸パン、総菜パン、菓子パン等の任意の形態のパンであってよい。本発明は、本発明に係るパン生地を用いた上記のような米粉パンの製造方法も提供する。本発明の米粉パンの製造方法はまた、本発明に係るパン生地を調製する工程をさらに含んでもよい。したがって本発明は、本発明のパン生地の調製方法によりパン生地を調製し、上記のように発酵させ、焼成することを含む米粉パンの製造方法も提供する。なお、本発明のパン製造方法では、原料の混合から発酵・焼成までの一連の作業を1つのホームベーカリーやパン製造ラインで連続して行ってもよい。 It is also possible to produce confectionery bread, cooked bread, etc. by further topping nuts, fruits, cream, syrup, etc., sandwiching prepared foods, ham, etc. on baked bread. The bread processed in this way is also included in the scope of the bread according to the present invention. The rice flour bread according to the present invention may be any form of bread, such as bread, rolls, round bread, side bread, confectionery bread and the like. The present invention also provides a method for producing the above-mentioned rice flour bread using the bread dough according to the present invention. The method for producing the rice flour bread of the present invention may further include the step of preparing the bread dough according to the present invention. Accordingly, the present invention also provides a method for producing rice flour bread comprising preparing bread dough according to the method for preparing bread dough of the present invention, fermenting as described above, and baking. In the bread manufacturing method of the present invention, a series of operations from mixing of raw materials to fermentation / baking may be performed continuously in one home bakery or bread manufacturing line.
本発明の方法によって本発明に係るパン生地から製造される米粉パンは、グルテン及び増粘剤を含まないにも関わらず、良好な膨らみを示す。本発明の方法では、米粉にグルタチオンを配合することなく、良好な膨らみを示すパン生地及び米粉パンを製造することができる。本発明に係る米粉パン、特にグルテンフリー米粉パンは、小麦粉などのグルテン構成タンパク質を含む穀物粉やグルテンを原料として使用せずに製造されることから、グルテン構成タンパク質やグルテンを含まず、このため、小麦アレルギー患者やセリアック病患者などグルテン摂取を回避すべき人に対し特に好適である。 The rice flour bread produced from the bread dough according to the present invention by the method of the present invention exhibits good puffiness despite the absence of gluten and thickeners. According to the method of the present invention, bread dough and rice flour bread can be produced which show good swelling without blending glutathione in rice flour. The rice flour bread according to the present invention, especially gluten-free rice flour bread, is manufactured without using gluten-containing protein or cereal flour containing gluten-constituting proteins such as wheat flour as a raw material, and therefore contains no gluten-constituting protein or gluten It is particularly suitable for people who should avoid gluten intake, such as wheat allergic patients and celiac disease patients.
以下、実施例を用いて本発明をさらに具体的に説明する。但し、本発明の技術的範囲はこれら実施例に限定されるものではない。 Hereinafter, the present invention will be more specifically described using examples. However, the technical scope of the present invention is not limited to these examples.
以下の実施例では、米粉として、うるち米を原材料とし、製菓用や製パン用米粉として市販されている試料1〜9の米粉を用いた。試料1〜9はいずれも、小麦粉等のグルテン構成タンパク質を含む穀物粉、グルテン構成タンパク質、グルテン及び増粘剤を含まない。塩化ナトリウム含有量99%以上の精製塩を食塩として使用した。
In the following examples, as rice flour, rice flour of
[実施例1]米粉パンの製造
ホームベーカリー(SD−BH105、パナソニック株式会社製)のパンケースに、試料1〜9のいずれかの米粉160g、水140gを入れ、「ドライイーストコース」のプログラムを用いて室温で20分間攪拌によりミキシングした。これに、砂糖を15g、パン用乾燥酵母(日清フーズ社製)を5g、バター(カルピス株式会社製)を2g、食塩を1.6g添加した後、「ドライイーストコース」のプログラムを用いてさらに室温で20分間攪拌によりミキシングした。このようにして得られた生地175gを角形パンケース(容量800mL)に計りとり、オーブンレンジ(EMO−C16C、三洋電機株式会社)で40℃、30分間程度発酵させた。この発酵段階の終了時にパン生地の外観を観察した。続いて、発酵段階を終了した生地(発酵生地)を180℃で24分間焼成して、グルテン及び増粘剤を含まない米粉パンを製造した。
Example 1 Production of Rice Flour Bread 160 g of rice flour of any of
焼成した翌日、各パンを、重量計測及びレーザー体積計(SELNAC−WinVM2100A、株式会社アステックス)による形状計測に供試し、パンの膨張性の指標である比容積(mL/g)の測定値を得た。比容積はパン1g当たりのパンの容積(mL/g)で表す。得られた結果を表1に示す。なお、表1には各米粉の製造に用いた製粉法も記載している。 The next day after baking, each bread was subjected to weight measurement and shape measurement with a laser volume meter (SELNAC-WinVM2100A, Astex Co., Ltd.), and the measured value of the specific volume (mL / g), which is an index of the expansivity of bread. Obtained. The specific volume is expressed as volume of bread per gram of bread (mL / g). The obtained results are shown in Table 1. Table 1 also describes the milling methods used to produce each rice flour.
表1に示すように、同じ製法で作製した米粉パンでも、使用する米粉によって製造されるパンの比容積が異なっていた。 As shown in Table 1, even with rice flour bread produced by the same method, the specific volume of the bread produced was different depending on the rice flour used.
さらに、それぞれのパンについて切断後の断面を写真撮影し、観察した(図1)。焼成後にパン比容積が4mL/g以上になったパン(試料1、2、及び7)は、いずれも焼成前の発酵段階終了時に角型パンケースの上部まで生地が膨らんでいた。発酵に供した生地の重量が175g、角形パンケースの容量が800mLであることから、試料1、2又は7を用いたパンは発酵段階終了時ですでに比容積が4mL/g以上になっていたことが示された。小麦粉を使用したパンは発酵後の焼成段階で大きく膨らむが、本発明に係る米粉パンは発酵段階で大きく膨らみ、焼成段階ではそれほど膨らまないことが示された。
Furthermore, the cross section after cutting about each bread was photographed and observed (FIG. 1). In each of the breads (
なお試料7の米粉を用いた場合、小麦粉やグルテンを原料に含むパンとよく似た、きめの整ったパンが製造された(図1G)。試料1は試料7に比べて柔らかく、ややつぶれやすいパンを提供した(図1A)。試料2を用いた場合にはパンに鬆(す)が入りやすかった(図1B)。
In addition, when the rice flour of sample 7 was used, a well-baked bread similar to a bread containing wheat flour and gluten as a raw material was manufactured (FIG. 1G).
小麦粉やグルテンを原料に用いた米粉パンの生地では、粘りのあるグルテンタンパク質のネットワークが発酵ガスを閉じ込めることによりパンが膨らむが、グルテンを含まない米粉パンはそのようなグルテンのネットワークを持たない。しかし本実施例での試験結果により、所定の性状を示す米粉を使用することで、グルテンを含有しなくても良好な膨らみを有する米粉パンを製造できることが示された。試料1、2及び7の米粉を使用したグルテンを含まない米粉パンは、グルテンを含む米粉パンとは異なるメカニズムにより良好な膨らみを達成したものと考えられる。
In the flour of rice flour bread using flour and gluten as raw materials, the network of sticky gluten protein causes the bread to swell by trapping the fermented gas, but the rice flour bread without gluten does not have such a network of gluten. However, according to the test results in this example, it was shown that rice flour bread having good swelling can be produced without using gluten, by using rice flour showing predetermined properties. It is considered that gluten-free rice flour bread using the rice flours of
[実施例2]米粉の性状評価とパン比容積との関係
グルテンも増粘剤も含まない米粉パンの比容積を高めることができる米粉の性状について検討するため、試料1〜9の米粉について損傷澱粉率、吸水率、及び最頻粒径を測定した。
[Example 2] Relation between evaluation of properties of rice flour and specific volume of bread In order to investigate the properties of rice flour capable of enhancing the specific volume of rice flour bread containing neither gluten nor thickener, damage to the rice flour of
1)損傷澱粉率の測定
損傷澱粉測定キット(Starch Damage Assay Kit; Megazyme社)を使用して、以下のようにして各米粉の損傷澱粉率を求めた。
1) Measurement of Damaged Starch Rate The damaged starch rate of each rice flour was determined as follows using a damaged starch measurement kit (Starch Damage Assay Kit; Megazyme).
まず、米粉100mgをチューブに入れ、このチューブ及びアミラーゼ溶液(50U/ml)を40℃で約5分、プレインキュベーションした。チューブ中の米粉に1mLのアミラーゼ溶液を加え、ボルテックスミキサーで混合し、40℃で10分間反応させた。硫酸溶液(0.2%v/v)を8ml添加し、ボルテックスミキサーで混合することにより、その反応を停止させた。次いで、3,000rpmで5分遠心した。上清の0.1mlを採取し、別のチューブに入れ、そこに0.1mlのアミログルコシダーゼ溶液を加えて40℃で10分間反応させた。さらに4mlのグルコース測定試薬GOPOD(グルコースオキシダーゼ及びペロキシダーゼ)溶液を加え、40℃で20分間反応させた。反応液について510nmで吸光度を測定した。一方、150mg/mlのグルコース標準液及び緩衝液(ブランク)についてGOPOD溶液を加えて反応させ、吸光度の測定を行い、検量線を作成した。この検量線に基づいて各米粉の損傷澱粉率(%)を決定した。
その結果を表2に示す。
First, 100 mg of rice flour was placed in a tube, and this tube and amylase solution (50 U / ml) were preincubated at 40 ° C. for about 5 minutes. 1 mL of amylase solution was added to rice flour in a tube, mixed with a vortex mixer, and allowed to react at 40 ° C. for 10 minutes. The reaction was stopped by adding 8 ml of sulfuric acid solution (0.2% v / v) and mixing with a vortex mixer. Then, it was centrifuged at 3,000 rpm for 5 minutes. 0.1 ml of the supernatant was collected and placed in another tube, to which 0.1 ml of amyloglucosidase solution was added and reacted at 40 ° C. for 10 minutes. Further, 4 ml of a glucose measuring reagent GOPOD (glucose oxidase and peroxidase) solution was added and reacted at 40 ° C. for 20 minutes. Absorbance was measured at 510 nm for the reaction solution. On the other hand, a GOPOD solution was added to and reacted with 150 mg / ml of glucose standard solution and buffer solution (blank), and the absorbance was measured to prepare a calibration curve. The percent damaged starch of each rice flour was determined based on this calibration curve.
The results are shown in Table 2.
2)吸水率の測定
米粉(乾燥状態)10gを50mLチューブに入れ、50mLの目盛までイオン交換水を添加した後、室温で一晩放置した。このチューブを3000rpmで30分間遠心した後、上清を捨て、吸水後の米粉重量(米粉と米粉に吸収された水の合計重量)を測定した。吸水率は以下の式で算出した。
吸水率(%)=(吸水後の米粉重量−吸水前の米粉重量(10g))/吸水前の米粉重量(10g)×100
各米粉について決定した吸水率を表2に示す。
2) Measurement of water absorption rate 10 g of rice flour (in a dry state) was placed in a 50 mL tube, ion exchange water was added to a scale of 50 mL, and left overnight at room temperature. The tube was centrifuged at 3000 rpm for 30 minutes, the supernatant was discarded, and the weight of rice flour after water absorption (total weight of rice flour and water absorbed by rice flour) was measured. The water absorption rate was calculated by the following equation.
Water absorption rate (%) = (weight of rice flour after water absorption-weight of rice flour before water absorption (10 g)) / weight of rice flour before water absorption (10 g) × 100
The water absorption determined for each rice flour is shown in Table 2.
3)最頻粒径の測定
各米粉のメーカーから提供されているスペック情報に最頻粒径が含まれる場合はそれを表2に示した。最頻粒径が開示されていない米粉については、粒度分布測定装置LS13320(ベックマンコールター)を使用し、マニュアルに従って乾式法による粒度分布の計測を行い、その粒度分布計測値から最頻粒径を求めた。この測定結果も表2に示す。
3) Measurement of Mode Particle Size If the specification information provided by the maker of each rice flour includes the mode particle size, it is shown in Table 2. For rice flour whose mode particle size is not disclosed, the particle size distribution is measured by the dry method according to the manual using a particle size distribution measuring apparatus LS13320 (Beckman Coulter), and the mode particle size is determined from the particle size distribution measurement value. The The measurement results are also shown in Table 2.
また、損傷澱粉率、吸水率、最頻粒径のそれぞれと、表1に示したパン比容積との関係を示すグラフを図2、図3、及び図4に示した。 Moreover, the graph which shows the relationship between each of a damaged starch rate, a water absorption, and the most frequent particle size, and the bread specific volume shown in Table 1 was shown in FIG.2, FIG.3, and FIG.4.
図2に示されるように、米粉の損傷澱粉率はパン比容積と強い相関を示した。特に、4mL/g以上のパン比容積を得るのに好適な米粉の損傷澱粉率は、5%以下であることが明らかとなった。 As shown in FIG. 2, the damaged starch rate of rice flour showed a strong correlation with the bread specific volume. In particular, it was found that the damaged starch rate of rice flour suitable for obtaining bread specific volume of 4 mL / g or more is 5% or less.
図3に示されるように、米粉の吸水率はパン比容積と強い相関を示した。特に、4mL/g以上のパン比容積を得るのに好適な米粉の吸水率は、80%以下であることが明らかとなった。 As shown in FIG. 3, the water absorption rate of rice flour showed a strong correlation with the bread specific volume. In particular, the water absorption of rice flour suitable for obtaining a bread specific volume of 4 mL / g or more was found to be 80% or less.
一方、図4に示されるように、米粉の粒径(特に、最頻粒径)はパン比容積とほとんど相関を示さなかった。 On the other hand, as shown in FIG. 4, the particle size of rice flour (particularly, the mode particle size) showed almost no correlation with the bread specific volume.
以上の結果から、5%以下の損傷澱粉率と80%以下の吸水率を示す米粉を米粉パンの製造に用いることにより、グルテンや増粘剤を使用しなくても、膨らみの良い米粉パンを製造できることが示された。グルテンを含有する米粉パンとは違い、グルテンを含まない米粉パンはグルテンネットワークを全く有しないことを考えると、このような米粉を使用することによりグルテンや増粘剤を含まない膨らみの良い米粉パンを製造できたことは驚くべき結果である。 From the above results, by using rice flour showing a damaged starch ratio of 5% or less and a water absorption rate of 80% or less for the production of rice flour bread, rice flour bread having a good swelling even without using gluten or thickeners. It has been shown that it can be manufactured. Unlike gluten-containing rice flour bread, considering that gluten-free rice flour bread has no gluten network, the use of such rice flour makes glutinous or thickener-free rice flour bread free of gluten and thickeners. The fact that it could be manufactured is a surprising result.
本発明を用いれば、グルテン及び増粘剤を含まない膨らみの良い米粉パンを容易に製造することができる。 Using the present invention, it is possible to easily produce glutinous and thickener free floured rice flour bread.
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