JP2019110875A - Composition - Google Patents

Abstract

To provide a composition that can effectively use saponarin, one of flavonoids, or a composition having an excellent calcium absorption promoting action as a new use for saponarin.SOLUTION: A composition contains 0.00001-20 mass%, preferably 0.0001-15 mass%, more preferably 0.001-10 mass% of saponarin, and 0.001-50 mass%, preferably 0.01-45 mass%, more preferably 0.1-40 mass% of calcium, and further contains unfermented tea and lactic acid bacteria.SELECTED DRAWING: Figure 1

Description

The present invention relates to a composition containing saponalin and calcium.

Saponarin is a kind of flavonoid and is conventionally known to be contained in the flowers of plants. As a plant containing this saponalin, mkuge is known, and the one obtained by drying the flower is used in traditional Chinese medicine as a xylophone. However, despite the fact that saponarin itself is known to be non-toxic and safe, it is hardly used at present. Thus, there is a need to develop new compositions that can effectively utilize saponalins.

Calcium, which is a divalent cation that plays an important role in the living body, is known as a major mineral that forms bones and teeth. For example, calcium that is absorbed in the intestine and enters the blood is used for bone metabolism, and in a healthy state, there is bone metabolism that is balanced by bone resorption and bone formation. However, due to a decrease in calcium intake and the like, an abnormality in bone metabolism causes a decrease in bone mass, resulting in bone-related diseases such as osteoporosis and fractures.

In addition, part of calcium in the body exists in cells and blood other than bones and teeth at a constant concentration, and plays an important role in terms of nerve and muscle excitation, blood coagulation, and the like. Therefore, when calcium in the blood is insufficient, diseases based on abnormalities of nerves, muscles, blood and the like, bone-related diseases due to progress of bone resorption, and the like will be seen.

Thus, calcium is a basic essential element of an organism, and plays an important role as a variety of physiological actions. If the combination of calcium and saponalin can be useful, it can greatly contribute to the effective use of saponalin.

Then, this invention makes it a subject which an invention tends to solve to provide the new composition which can utilize saponarin effectively. Another object of the invention is to provide a composition having a calcium absorption promoting action as a new utility of saponarin.

As a result of intensive investigations in light of the above problems, the present applicant has found that combining saponalin and calcium, which is known to be nontoxic and safe, exhibits an excellent calcium absorption promoting action. The present invention has been achieved.

The summary of the present invention is as follows.
<1> A composition containing saponalin and calcium.
<2> The composition according to <1>, further comprising unfermented tea.
<3> The composition according to <2>, further comprising a lactic acid bacterium.

The composition in any one of <1>-<3> which is for <4> calcium absorption promotion.
The composition in any one of <1>-<4> which is <5> food-drinks.

The compositions of the present invention can provide new utilities for saponalins. In addition, the composition containing saponalin and calcium of the present invention can provide an excellent calcium absorption promoting action.

It is a figure showing the change value of the amount of calcium absorption to comparative example 1 of Examples 1-4.

Hereinafter, the composition of the present invention will be described. The present invention is not limited to the following embodiment.

(A) Calcium Calcium is a metal element represented by the symbol of element Ca, and refers not only to calcium alone but also to a compound in which calcium and another element or anion are bonded. Calcium is not particularly limited as long as it is generally known, and commercially available inorganic salts or organic salts such as calcium carbonate, calcium silicate, calcium phosphate and calcium chloride are used. Among these, calcium carbonate is preferable from the viewpoint of stability and flavor. When calcium carbonate is used, its origin is not particularly limited, but eggshell calcium, scallop calcium, coral calcium and the like are preferable, and coral calcium is particularly preferable from the viewpoint of high safety. These calcium can be used individually by 1 type or in combination of 2 or more types.

There is no restriction | limiting in particular as content of calcium mix | blended with the composition of this invention, According to various conditions, such as an objective, a shape, and use object, the content can be suitably set in a wide range. For example, it is selected in the range of 0.001 to 50% by mass, preferably 0.01 to 45% by mass, and more preferably 0.1 to 40% by mass. In the present invention, commercially available products may be used, and those produced by methods known in the art may also be used.

(B) Saponarin Saponarin (CAS number: 20310-89-8) refers to a 7-O-glucoside of isobitexin (saponaretin), which is a mauve, red sea bream, red sea bream, bonito flower, sun flower, saponaria (soap grass), barley It is known to be included in plants such as sugar cane and pheasant. The saponalin used in the present invention is not particularly limited, and may be produced by a method generally known by those skilled in the art or may be a commercially available product. For saponalins, plants containing saponarins, such as flowers, leaves, stems, or processed products obtained by subjecting them to crushing or crushing may be used directly, or they may be separated / extracted or artificially synthesized, etc. You may use what is obtained by a method.

The crushing / pulverizing method for obtaining saponalin from the raw material is not particularly limited, and either wet or dry may be used, and the pulverizing conditions and the treatment apparatus are not particularly limited, and commercially available apparatuses and the like can be appropriately used. As an apparatus to be used, for example, a high pressure homogenizer, an ultrasonic crusher, an air flow crusher, a high speed rotary impact crusher, a ball mill, a bead mill or the like can be mentioned. These treatments may be repeated a plurality of times as necessary so that physical properties such as the particle diameter of the processed product fall within a preferable range, and a plurality of treatments may be combined. Further, the method for extracting and separating saponalin from the raw material and the synthesis method are not particularly limited, and can be appropriately selected according to the purpose. Examples of the extraction method include a method of adding an extraction solvent usually used by those skilled in the art such as ethanol, water, water-containing ethanol and the like, and heating and extracting as necessary.

There is no restriction | limiting in particular as content of the saponarin mix | blended with the composition of this invention, According to various conditions, such as an objective, a shape, and use object, the content can be set suitably over a wide range. For example, it is selected in the range of 0.00001 to 20% by mass, preferably 0.0001 to 15% by mass, and more preferably 0.001 to 10% by mass.

(C) Unfermented Tea The infermented tea used in the present invention is a leaf of a plant of camellia, tea tree genus Camellia (Camellia sinensis), that is, green tea derived from tea leaves, and as green tea, for example, matcha or green tea There are apricot tea, sencha, capricha, bancha, stem tea, powdered tea and so on.

The non-fermented tea used in the present invention is a dried tea leaf, a crushed tea leaf and its dried powder (ground powder), a tea leaf juice and its dried powder (juice powder), a tea leaf extract and its dried powder (extract End) and so on. In the case of obtaining a ground powder of tea leaves, the method of grinding treatment is not particularly limited, and either wet or dry treatment may be used, and the grinding conditions and the treatment apparatus are not particularly limited, and commercially available devices and the like can be used appropriately. As an apparatus to be used, for example, a high pressure homogenizer, an ultrasonic crusher, an air flow crusher, a high speed rotary impact crusher, a ball mill, a bead mill or the like can be mentioned. These treatments may be repeated a plurality of times as necessary so that physical properties such as the particle diameter of the processed product fall within a preferable range, and a plurality of treatments may be combined. When the tea leaf extract is obtained, the extraction solvent is not particularly limited, and examples thereof include solvents such as water, alcohol (for example, methanol, ethanol, n-propanol, n-butanol and the like), acetone and the like, and preferably Water and / or ethanol can be used. Moreover, when obtaining the dry powder, it can obtain by removing the solvent of an extract, for example by methods normally used by persons skilled in the art, such as reduced-pressure drying and spray-drying.

The method for producing unfermented tea is not particularly limited, and can be obtained by methods commonly used by those skilled in the art. In the present invention, in view of processability and stability, powder of unfermented tea is preferably used, and it is particularly preferable to use extract powder and pulverized powder, and pulverization in which ingredients contained in tea leaves can be consumed as it is Ginseng is more preferable, and in particular, Matcha is preferable.

There is no restriction | limiting in particular as content of the non-fermented tea mix | blended with the composition of this invention, According to various conditions, such as an objective, a shape, and use object, the content can be set suitably over a wide range. For example, it is selected in the range of 0.01 to 40% by mass, preferably 0.05 to 30% by mass, and more preferably 0.01 to 25% by mass. In the present invention, commercially available products may be used, and those produced by methods known in the art may also be used.

(D) Lactic acid bacteria Lactic acid bacteria refer to bacteria that produce lactic acid by metabolism. Lactic acid bacteria are not particularly limited as long as they are generally known, and for example, Streptococcus faecalis (sometimes referred to as Enterococcus faecalis), Lactobacillus acidophilus, Lactobacillus casei, Streptococcus thermophilus, Bacillus coagullans, etc. may be used. Streptococcus faecalis is particularly preferable from the viewpoints of human origin and high safety. These lactic acid bacteria can be used singly or in combination of two or more. There is no restriction | limiting in particular as a method of obtaining these lactic acid bacteria, For example, you may use lactic acid bacteria isolated from foodstuffs, such as yogurt and vegetables, and a commercial item.

There is no restriction | limiting in particular as content of the lactic acid bacteria mix | blended with the composition of this invention, According to various conditions, such as an objective, a shape, and use object, the content can be set suitably in a wide range. For example, it is selected in the range of 0.0001 to 20% by mass, preferably 0.001 to 15% by mass, and more preferably 0.01 to 10% by mass.

The compounding ratio (mass ratio) of (A) calcium and (B) saponalin in the composition of the present invention is not particularly limited, and can be appropriately set according to the conditions such as the purpose and the use object, but enhances the effect of the present invention From the viewpoint, for example, (A): (B) = 1: 0.000001 to 100, preferably 1: 0.00001 to 50, more preferably 1: 0.0001 to 30.

The compounding ratio (mass ratio) of (A) calcium and (B) saponalin and (C) infermented tea in the composition of the present invention is not particularly limited, and can be appropriately set according to the conditions such as the purpose and object of use. From the viewpoint of enhancing the effects of the present invention, for example, (A) + (B) :( C) = 1: 0.00001 to 100, preferably 1: 0.0001 to 50, more preferably 1: 0.001 to It is selected in the range of 30.

The blending ratio (mass ratio) of (A) calcium and (B) saponalin to (D) lactic acid bacteria in the composition of the present invention is not particularly limited, and can be appropriately set according to the conditions such as the purpose and the use target. From the viewpoint of enhancing the effects of the invention, for example, (A) + (B) :( D) = 1: 0.001 to 100, preferably 1: 0.01 to 50, more preferably 1: 0.1 to 30 It is selected by the range.

Although the compounding ratio (mass ratio) of (B) saponalin and (C) infermented tea in the composition of this invention is not specifically limited, According to conditions, such as an objective and a use object, it can set suitably, for example From the viewpoint of enhancing the effect, it is selected in the range of (B) :( C) = 1: 0.001 to 150, preferably 1: 0.01 to 100, more preferably 1: 0.1 to 50.

The compounding ratio (mass ratio) of (B) saponalin and (D) lactic acid bacteria in the composition of the present invention is not particularly limited, and can be appropriately set according to the conditions such as the purpose and the use object. From the viewpoint of enhancing (B): (D) = 1: 0.001 to 200, preferably 1: 1: 0.01 to 150, more preferably 1: 1: 0.1 to 125.

The compounding ratio (mass ratio) of (C) infermented tea to (D) lactic acid bacteria in the composition of the present invention is not particularly limited, and can be appropriately set according to the conditions such as the purpose and the use object. From the viewpoint of enhancing the effect, it is selected in the range of (C) :( D) = 1: 0.0001 to 100, preferably 1: 0.001 to 50, and more preferably 1: 0.01 to 30.

The composition of the present invention may contain other components in addition to calcium, saponalin, unfermented tea and lactic acid bacteria. As said other components, microorganisms, such as protein, processed products of plants or plants, algae, yeast, etc. can be mix | blended, for example. Furthermore, dextrin, saccharides such as starch, sweeteners, acidulants, colorants, thickeners, brighteners, excipients, vitamins, nutritional supplements, binders, lubricants, which are usually used in the food field, if necessary. There may be mentioned fillers, stabilizers, diluents, fillers, emulsifiers, food additives, seasonings and the like. The content of these other components can be appropriately selected according to the form of the composition of the present invention and the like.

The form of the composition of the present invention is not particularly limited and can be any form. As a specific form, for example, a powder, such as powder, granules, powder such as fine granules, a tablet (chewable), a sphere, a capsule, a caplet, a liquid or the like can be mentioned. The capsule-like composition includes soft capsules and hard capsules. The composition of the present invention is preferably in the form of powder, such as powder, granules, fine particles, etc., and in particular, it is mixed with water or the like and dissolved or suspended to form a powdered beverage to be used. It is preferable because it is excellent in stability and can take many compositions at a time unlike capsules and tablets.

The composition of the present invention can be produced by a conventionally known method. When manufacturing the composition of this invention, the form of the raw material to be used is not specifically limited, According to the form of a composition, it can select suitably and can be used. For example, in the case of obtaining a powdery composition such as powder, granules or fine granules, calcium, saponalin, infermented tea and lactic acid bacteria may be used as they are, or they may be mixed with excipients, extenders, etc. You may use Moreover, when obtaining a capsule-like composition, you may use what was melt | dissolved or disperse | distributed beforehand in solvent, such as water and an edible oil.

As a method of effectively using the composition of the present invention, displaying and using the effect obtained by taking calcium into the body of the product using the composition of the present invention, a package, an instruction, or a promotional item Can. For example, as products, so-called health food such as pharmaceuticals (including quasi-drugs), food for specified health use, food for nutritional function, food for functional indication, etc. Etc. In so-called health food products, those displaying "for maintaining bone health", "for those concerned about bone health", "for those concerned about dental health", "for calcium supplementation", etc. It can be illustrated.

Since the composition of the present invention can promote calcium absorption, it can be used as a calcium absorption promoting composition, a composition for calcium absorption support, a composition for calcium supplement, a composition for calcium intake, etc. Food and drink composition for promoting, food and drink composition for calcium absorption support, food and drink composition for calcium supplement, food and drink composition for calcium intake, agent for calcium absorption promotion, agent for calcium absorption support, agent for calcium supplement, calcium It can also be used as an intake agent.

Hereinafter, the present invention will be specifically described by way of examples. The present invention is not limited to these examples.

[Test example calcium absorption test]
(Test method)
Human colon adenocarcinoma-derived cells (Caco-2) were seeded and cultured at a cell density of 4.0 × 10 ⁴ cells / well in insert wells (mucosal wells) attached to a 24-well plate (basal membrane side wells). The medium used was DMEM medium supplemented with 10% FBS and 1% non-essential Amino Acid Solution. The cells were cultured in a 5% CO ₂ incubator for 14 to 15 days to prepare Caco-2 monolayer membranes.

After washing the insert well with calcium-free buffer (Hanks balanced salt solution, 10 mM HEPES), transfer it to a 24-well plate containing 1000 μL of Buffer, add 350 μL of buffer to the insert well and acclimate in an incubator for 30 minutes. The

After habituation, a Buffer in the insert wells was 175μL removed, each solution of the test substance was prepared according to the method described below, a total of 175μL was added to the insert wells to a final concentration shown in Table 1 below, CO ₂ The cells were cultured for 3 hours in an incubator.
-Calcium or lactic acid bacteria: prepared in a solution of 10 mg / mL with Buffer containing 0.5% DMSO, and used appropriately diluted with Buffer containing 0.5% DMSO.
Saponarin: dissolved in 200 mg / mL in 100% DMSO, then prepared into a 1 mg / mL solution in Buffer, and appropriately diluted in Buffer containing 0.5% DMSO and used.
· Infermented tea: After preparation to 10 mg / mL with Buffer containing 0.5% DMSO, the supernatant (solution) obtained by centrifuging at 15,000 rpm for 5 minutes is collected, and Buffered with 0.5% DMSO. It diluted suitably and used.
In addition, about unfermented tea, it added to the insert well so that it might become the final concentration shown in Table 1 based on the density | concentration (10 mg / mL) before solution collection | recovery. As a control, 175 μL of buffer containing 0.5% DMSO containing no calcium was added.

After incubation, collect 24 well plate of Buffer, concentrate to a fixed volume with a centrifugal evaporator (manufactured by Tokyo Rika Kikai Co., Ltd.), and measure metalloassay calcium (Ca). The amount of calcium that permeated the Caco-2 monolayer membrane was measured using this. The value which deducted the value of control was computed from the value of each measured group, and the relative value when the value of a comparative example was set to 100 was computed. In addition, since calcium which permeate | transmitted the intestinal tract is absorbed in the body, the amount of calcium which permeate | transmitted Caco-2 single layer film is hereafter described as absorption.

Moreover, the change value from the calcium absorption amount of a comparative example was computed by the following formula, and the calcium absorption promotion effect was evaluated.
Change value of calcium absorption amount = (calcium absorption amount of sample to which each test substance was added)-100

(Measurement result)
The test substances in each test area are shown in Table 1. The numerical value in each test section indicates the final concentration of each component, and all units are μg / mL. As a test substance, calcium is commercially available powdered coral calcium (calcium standard value 10%), saponalin is commercially available reagent, unfermented tea is commercially available matcha green tea, and lactic acid bacteria are commercially available Powdered dried Streptococcus faecalis dry cells were used respectively.

The change value from the calcium absorption of the comparative example is shown in the graph of FIG. As shown in FIG. 1, Example 1 containing coral calcium and saponalin had higher calcium absorption than Comparative Example 1 containing only coral calcium. Example 2 which contains matcha calcium in addition to coral calcium and saponarin has a higher calcium absorption even when compared to Example 1 which does not contain matcha. On the other hand, Example 3 which contains lactic acid bacteria in addition to coral calcium and saponal does not have a high calcium absorption amount as in Example 2, whereas the change value of Example 2 is 27.0, but is performed The change value of Example 2 was 14.1 which is lower than the change value 17.7 of Example 1. However, it is surprising that Example 4 containing coral tea and saponalin in addition to matcha and lactic acid bacteria has a change in calcium absorption that is about twice as high as that in Example 2 and Comparative Example 1 It is more than 50% higher than the amount absorbed. Although the reason is unknown, it can be speculated that the combination of saponalin, matcha and lactic acid bacteria may have a synergistic effect.

From the above results, the composition containing calcium and saponalin (composition II) is superior to the composition containing calcium alone (composition I) in the calcium absorption promoting action, and in addition to the composition II, matcha is added to the composition II. It was found that the composition containing (Composition III) is further excellent in the calcium absorption promoting action. On the other hand, it has been found that the composition containing the lactic acid bacteria in addition to the composition II (composition IV) does not enhance the calcium absorption promoting effect of saponalin like the composition III but rather reduces it. However, unexpectedly, in addition to the composition II, the composition containing the green tea and the lactic acid bacteria (composition V) was found to be extremely excellent in the calcium absorption promoting action.

Examples 5 to 34 (Production of Powdered Beverages)
After the raw materials were prepared according to the compounding ratio described in the following formulation example, a powdered beverage exhibiting the effects of the present invention was manufactured according to a conventional method.

According to the present invention, it is possible to provide new utility of saponarin. In addition, a composition having an excellent calcium absorption promoting action can be provided.

Claims (2)

A composition containing saponalin and calcium. The composition according to claim 1, further comprising unfermented tea.