JP2019089742A - Biofilm dispersion remover - Google Patents
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- JP2019089742A JP2019089742A JP2017221450A JP2017221450A JP2019089742A JP 2019089742 A JP2019089742 A JP 2019089742A JP 2017221450 A JP2017221450 A JP 2017221450A JP 2017221450 A JP2017221450 A JP 2017221450A JP 2019089742 A JP2019089742 A JP 2019089742A
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Landscapes
- Cosmetics (AREA)
Abstract
Description
本発明は、バイオフィルム分散除去剤に関する。 The present invention relates to a biofilm dispersion remover.
頭皮臭は、酸臭と皮脂臭から構成されており、このうち酸臭が頭皮臭の強度に大きく寄与していることが知られている。この酸臭の原因物質は、イソ吉草酸に代表される低級脂肪酸であり、皮膚の代謝産物であるロイシンから頭皮に棲息する常在菌の関与によって産生されることが知られている。このため、頭皮臭の発生を防止又は軽減するために、ヒドロキシピリドン系化合物、ピリチオン系化合物、ミコナゾール、イオウ等の殺菌剤をシャンプー等の毛髪化粧料に配合することが行われている。 The scalp odor is composed of an acid odor and a sebum odor, among which it is known that the acid odor greatly contributes to the intensity of the scalp odor. The causative agent of this acid odor is a lower fatty acid typified by isovaleric acid, and is known to be produced by the involvement of a resident bacteria that inhabits the scalp from leucine which is a skin metabolite. For this reason, in order to prevent or reduce the occurrence of scalp odor, it has been practiced to add a bactericidal agent such as a hydroxypyridone compound, a pyrithione compound, miconazole or sulfur to a hair cosmetic such as a shampoo.
しかし、自然界に存在する細菌は、１つ１つが個別に浮遊菌の状態で存在するのではなく、何らかの表面に付着し、多糖、タンパク質等の細胞外ポリマーを産生、分泌しながら増殖しバイオフィルムを形成する。そして形成されたバイオフィルムに対しては、殺菌剤、抗菌剤はその効果を発揮しにくくなることが知られている。 However, naturally occurring bacteria are not individually present individually in suspension, but attached to some surface, proliferate while producing and secreting extracellular polymers such as polysaccharides and proteins, and then grown into a biofilm. Form Then, it is known that a bactericidal agent and an antibacterial agent hardly exert their effect on the formed biofilm.
そこで、バイオフィルムの形成抑制や、形成されたバイオフィルムを除去するため、種々の提案がされている。なかでも、安全性が高い化合物を利用した技術として、例えば、特許文献１では、特定の界面活性剤と特定のlogP値を有するアルコールを併用したバイオフィルムの除去方法が開示されている。特許文献２には、ファルネソールとキシリトールの併用によりバイオフィルムの形成を抑制することが開示されている。特許文献３には、特定の香料素材を用いたバイオフィルム抑制剤が開示されている。 Therefore, various proposals have been made to suppress the formation of a biofilm and remove the formed biofilm. Among them, as a technique using a highly safe compound, for example, Patent Document 1 discloses a method of removing a biofilm using a specific surfactant and an alcohol having a specific logP value in combination. Patent Document 2 discloses that the combined use of farnesol and xylitol suppresses the formation of a biofilm. Patent Document 3 discloses a biofilm inhibitor using a specific spice material.
しかし、特許文献１に記載の除去方法では効果は十分とはいえず、また特許文献２、３に記載のようなバイオフィルムの形成抑制技術は、いったん形成してしまったバイオフィルムに対しては有効なものではない。 However, the removal method described in Patent Document 1 is not sufficient in effect, and the biofilm formation suppression technology as described in Patent Documents 2 and 3 is applied to a biofilm that has once been formed. It is not effective.
したがって本発明は、汎用性が高く、頭皮上のバイオフィルムを分散除去することができ、頭皮の臭いを低減することができるバイオフィルム分散除去剤に関する。 Therefore, the present invention relates to a biofilm dispersion removal agent which is highly versatile, can disperse and remove biofilm on the scalp, and can reduce the odor of the scalp.
本発明者らは、汎用性が高く、人体に対し使用実績のある化合物を用いた、頭皮に棲息する常在菌であるスタフィロコッカス属細菌に由来するバイオフィルムの分散除去力を種々検討した結果、特定の選択肢から選ばれる香料素材が前記要求を備えるものであることを見出した。 The present inventors variously examined the dispersion removal ability of a biofilm derived from Staphylococcus bacteria, which is a resident bacteria living on the scalp, using a compound which is highly versatile and has been used in the human body. As a result, it has been found that a spice material selected from a specific option is one having the above-mentioned requirement.
本発明は、エチルトリシクロ [5.2.1.02.6] デカン-2-イル カルボキシレート及びエチル2-シクロヘキシル プロピオネートから選ばれる１種又は２種の化合物を有効成分とする、スタフィロコッカス属細菌に由来するバイオフィルムの分散除去剤を提供するものである。 The present invention is derived from Staphylococcus bacteria comprising as an active ingredient one or two compounds selected from ethyltricyclo [5.2.1.0 2.6 ] decan-2-yl carboxylate and ethyl 2-cyclohexyl propionate. It is intended to provide a biofilm remover.
更に本発明は、前記バイオフィルム分散除去剤を含有する頭髪用化粧料を提供するものである。 Furthermore, the present invention provides a hair cosmetic comprising the biofilm dispersion remover.
更に本発明は、前記バイオフィルム分散除去剤又は前記頭髪用化粧料を生物表面に形成されたスタフィロコッカス属細菌のバイオフィルムに対して適用する、頭皮臭の低減方法を提供するものである。 Furthermore, the present invention provides a method for reducing scalp odor, which comprises applying the biofilm dispersion remover or the hair cosmetic composition to a biofilm of Staphylococcus bacteria formed on a biological surface.
本発明のバイオフィルム分散除去剤は、汎用性が高く、低濃度で頭皮常在菌であるスタフィロコッカス属細菌に由来するバイオフィルムを分散除去することができ、頭皮の細菌叢を乱すことなく頭皮を正常に保ち、前記常在菌が生成する低級脂肪酸のニオイを長期的に低減することができる。 The biofilm dispersion removal agent of the present invention is highly versatile, can disperse and remove biofilms derived from Staphylococcus bacteria that are indigenous to the scalp at low concentrations, and does not disturb the bacterial flora of the scalp The scalp can be kept normal, and the odor of lower fatty acids produced by the above-mentioned indigenous bacteria can be reduced over time.
本発明のバイオフィルム分散除去剤は、エチルトリシクロ [5.2.1.02.6] デカン-2-イル カルボキシレート（フルテート、登録商標；花王社製）及びエチル2-シクロヘキシル プロピオネート（ポワレネート、登録商標；花王社製）から選ばれる１種又は２種の化合物を有効成分とするものであり、これらの化合物はスタフィロコッカス属細菌に由来するバイオフィルムの分散性に優れる。これらはいずれも香料として用いられている公知のエステル化合物であり、一般的な化学合成によって製造されたものを使用することができる。 The biofilm dispersion removing agent of the present invention includes ethyl tricyclo [5.2.1.0 2.6 ] decan-2-yl carboxylate (flute) (registered trademark; manufactured by Kao Corporation) and ethyl 2-cyclohexyl propionate (pourenate, registered trademark; Kao Corporation). Or the like as an active ingredient, and these compounds are excellent in the dispersibility of a biofilm derived from Staphylococcus bacteria. These are all known ester compounds used as perfumes, and those produced by general chemical synthesis can be used.
本発明で対象とするバイオフィルムを形成するスタフィロコッカス属細菌としては、生物表面、特に頭皮より分離される常在菌、具体的にはスタフィロコッカス・キャピティス（Staphylococcus capitis；以下、S.capitisと記載する場合もある）が挙げられる。 Staphylococcus bacteria forming biofilms targeted by the present invention include indigenous bacteria isolated from the surface of organisms, in particular from the scalp, specifically Staphylococcus capitis; hereinafter, S. capitis May be described).
本発明のバイオフィルム分散除去剤は、頭髪用化粧料中に含有させて使用することができる。頭髪化粧料としては、シャンプー、コンディショナー、リンス、清拭シートが挙げられる。 The biofilm dispersion removing agent of the present invention can be used by being contained in a hair cosmetic. Hair cosmetics include shampoos, conditioners, rinses, and wipes.
頭髪化粧料中における本発明のバイオフィルム分散除去剤の含有量は、エチルトリシクロ[5.2.1.02.6] デカン-2-イル カルボキシレート及びエチル2-シクロヘキシル プロピオネートの合計量として、シャンプー、コンディショナー、リンスの場合、好ましくは0.01質量％以上、より好ましくは0.03質量％以上、更に好ましくは0.05質量％以上であり、また、好ましくは８質量％以下、より好ましくは５質量％以下、更に好ましくは３質量％以下である。また、清拭シートの場合は、シートに含浸させる水性洗浄剤組成物中における含有量として、上記範囲が好ましい。 The content of the biofilm dispersion removing agent of the present invention in the hair cosmetic composition is shampoo, conditioner, rinse, as a total amount of ethyltricyclo [5.2.1.0 2.6 ] decan-2-yl carboxylate and ethyl 2-cyclohexyl propionate. Is preferably 0.01% by mass or more, more preferably 0.03% by mass or more, still more preferably 0.05% by mass or more, preferably 8% by mass or less, more preferably 5% by mass or less, still more preferably 3% by mass % Or less. Moreover, in the case of a wiping sheet, the said range is preferable as content in the aqueous | water-based washing agent composition with which a sheet | seat is impregnated.
これらの頭髪用化粧料には、通常の頭髪用化粧料に用いられる成分を目的、用途、剤型等に応じて適宜配合できる。このような成分としては、例えば、水、低級アルコール等の媒体；アニオン界面活性剤、カチオン界面活性剤、非イオン界面活性剤、両性界面活性剤等の界面活性剤；炭化水素、ロウ、高級アルコール、エステル油、高級脂肪酸等の油剤；ジンクピリチオン、オクトピロックス等の抗フケ剤；ビタミン剤；グリチルリチン酸ジカリウム、酢酸トコフェロール等の抗炎症剤；メチルパラベン、ブチルパラベン等の防腐剤；キレート剤；パンテノール等の保湿剤；染料、顔料等の着色剤；ヒドロキシエチルセルロース、メチルセルロース、ポリエチレングリコール、粘土鉱物等の粘度調整剤；有機酸、水酸化ナトリウム、水酸化カリウム等のpH調整剤；植物エキス類；パール化剤；香料；色素；紫外線吸収剤；酸化防止剤；その他エンサイクロペディア・オブ・シャンプー・イングリーディエンツ〔ENCYCLOPEDIA OF SHAMPOO INGREDIENTS (MICELLE PRESS)〕に記載されている成分等が挙げられる。 These hair cosmetic compositions can be appropriately blended with components generally used for hair cosmetic compositions according to the purpose, application, agent type and the like. Such components include, for example, media such as water and lower alcohols; anionic surfactants, cationic surfactants, nonionic surfactants, surfactants such as amphoteric surfactants; hydrocarbons, waxes, and higher alcohols. Oils such as ester oil and higher fatty acids Anti-dandruff agents such as zinc pyrithione and octopirox Vitamins Anti-inflammatory agents such as dipotassium glycyrrhizinate and tocopherol acetate Antiseptics such as methyl paraben and butyl paraben Chelate agent Panthenol Humectants such as dyes; Colorants such as dyes and pigments; Viscosity modifiers such as hydroxyethyl cellulose, methyl cellulose, polyethylene glycol and clay minerals; pH modifiers such as organic acids, sodium hydroxide and potassium hydroxide; Plant extracts; Agents; perfumes; dyes; UV absorbers; antioxidants; Components, etc. that are described in and breakfasts Of Shampoo Inn greedy Ingredients [ENCYCLOPEDIA OF SHAMPOO INGREDIENTS (MICELLE PRESS)] and the like.
本発明のバイオフィルム分散除去剤又はこれを含有する頭髪用化粧料を生物表面、特に頭皮に形成されたスタフィロコッカス属細菌のバイオフィルムに対して適用し、洗い流すことにより、バイオフィルムを除去して頭皮臭を低減することができる。 The biofilm dispersion removing agent of the present invention or a hair cosmetic containing the same is applied to a biofilm of Staphylococcus bacteria formed on a biological surface, particularly on the scalp, and the biofilm is removed by washing away. Can reduce the odor of the scalp.
本発明のバイオフィルム分散除去剤又は頭髪化粧料の生物表面への適用量は、バイオフィルムの分散除去効果、皮膚刺激抑制の観点から、皮膚表面の１cm2あたりのエチルトリシクロ [5.2.1.02.6] デカン-2-イル カルボキシレート及びエチル2-シクロヘキシル プロピオネートの合計量として、好ましくは0.001mg/cm3以上、より好ましくは0.002mg/cm3以上、更に好ましくは0.003mg/cm3以上であり、また、好ましくは0.1mg/cm3以下、より好ましくは0.07mg/cm3以下、更に好ましくは0.05mg/cm3以下である。 The amount of the biofilm dispersion removing agent or the hair cosmetic composition of the present invention applied to the biological surface is ethyltricyclo per 1 cm 2 of the skin surface from the viewpoint of the dispersing removal effect of the biofilm and suppression of skin irritation [5.2.1.0 2.6 ] as the total amount of the decane-2-yl carboxylate and ethyl 2-cyclohexyl propionate, preferably 0.001 mg / cm 3 or more, more preferably 0.002 mg / cm 3 or more, more preferably 0.003 mg / cm 3 or more, Further, it is preferably 0.1 mg / cm 3 or less, more preferably 0.07 mg / cm 3 or less, and still more preferably 0.05 mg / cm 3 or less.
実施例１〜２、比較例１〜３
１．悪臭（イソ吉草酸）の定量と官能評価
(1) S. capitis（頭皮分離株）菌液をTSB培地に1.0×106CFU/mLになるように添加し、96穴ウェル中にて24時間37℃で静置培養してバイオフィルムを形成させた。表１に示す各処方液を無菌的にバイオフィルムに接触させた後、イオン交換水で洗浄し洗浄液を廃棄した。
(2) 96穴ウェルに残留したバイオフィルムに0.2％L-ロイシン（東京化成工業社製）添加培地を３時間接触させ、ジエチルエーテル（和光純薬工業社製）で培養液を抽出した。ジエチルエーテル部分を0.5mLバイアルに充填し、イソ吉草酸をGC/MSで定量した。また、イソ吉草酸の臭いを対象とした下記基準による官能評価により、悪臭強度を評価した。これらの結果を表１に示す。
Examples 1-2, comparative examples 1-3
1. Determination of malodor (isovaleric acid) and sensory evaluation
(1) Add S. capitis (scalp isolate) bacterial solution to TSB medium to 1.0 × 10 6 CFU / mL, and incubate in a 96-well well for 24 hours at 37 ° C. to make a biofilm It was formed. After each formulation shown in Table 1 was aseptically brought into contact with the biofilm, it was washed with ion exchange water and the washing solution was discarded.
(2) The biofilm remaining in the 96-well was brought into contact with a 0.2% L-leucine (manufactured by Tokyo Chemical Industry Co., Ltd.)-Added culture medium for 3 hours, and the culture solution was extracted with diethyl ether (manufactured by Wako Pure Chemical Industries, Ltd.). The diethyl ether portion was filled into 0.5 mL vials and isovaleric acid was quantified by GC / MS. Moreover, malodor intensity was evaluated by sensory evaluation according to the following criteria for the odor of isovaleric acid. The results are shown in Table 1.
（悪臭強度官能評価スコア）
３：イソ吉草酸の臭いが楽にわかる
２：イソ吉草酸の臭いを弱く感じる
１：イソ吉草酸の臭いを感じない
(Odor odor intensity sensory evaluation score)
3: The smell of isovaleric acid is easily understood 2: The smell of isovaleric acid is weakly felt 1: The smell of isovaleric acid is not felt
２．バイオフィルム除去率
前記１(1)の後、96穴ウェルに残留したバイオフィルムをクリスタルバイオレット0.1％水溶液で染色した。イオン交換水を用いて各ウェルを２回ずつ洗浄後、99.5％エタノールを200μLウェルに添加して、染色したバイオフィルムを溶出し、570nmの吸光度を計測してバイオフィルムの残存量を算出した。
コントロール（比較例４）のバイオフィルム残存量を100％としたときの各実施例及び各比較例でのバイオフィルム残存量を差し引いた値をバイオフィルム除去率とした。また、このバイオフィルム除去率から、下記基準に従ってバイオフィルム除去能の判定を行った。
2. Biofilm Removal Rate After the above 1 (1), the biofilm remaining in the 96-well was stained with a 0.1% aqueous solution of crystal violet. After washing each well twice with ion-exchanged water, 99.5% ethanol was added to 200 μL of the wells, the stained biofilm was eluted, and the absorbance at 570 nm was measured to calculate the remaining amount of biofilm.
The value obtained by subtracting the residual amount of biofilm in each Example and Comparative Example when the residual amount of biofilm in the control (Comparative Example 4) was 100% was defined as the biofilm removal rate. Moreover, the biofilm removal ability was judged from this biofilm removal rate according to the following reference | standard.
（バイオフィルム除去能）
○：バイオフィルム除去率30％以上
△：バイオフィルム除去率25％以上30％未満
×：バイオフィルム除去率25％未満
(Biofilm removal ability)
○: biofilm removal rate of 30% or more Δ: biofilm removal rate of 25% or more and less than 30% ×: biofilm removal rate of less than 25%
処方例１〜２（毛髪洗浄剤）
毛髪洗浄剤の処方例を表２に示す。
Formulation Examples 1 and 2 (Hair Cleaner)
Table 2 shows formulation examples of the hair cleanser.
処方例３（毛髪洗浄シート）
毛髪洗浄シートの処方例を表３に示す。
Prescription example 3 (hair wash sheet)
Table 3 shows a formulation example of the hair washing sheet.
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