JP2019069163A - Space sterilization method - Google Patents

Abstract

To provide a space sterilization method capable of sterilizing and deodorizing sufficiently a space to be treated, without receiving a harmful effect of hypochlorous acid water.SOLUTION: In a space sterilization method in an embodiment, which is a space sterilization method for performing sterilization by supplying gaseous and/or misty hypochlorous acid water into a space to be treated, following formula (1) is satisfied in a relation between a germicidal treatment time y (time) and a supply amount x(μg/(m-min) of gaseous and/or misty hypochlorous acid water supplied to a unit area per unit time.SELECTED DRAWING: Figure 4

Description

  Embodiments of the present invention relate to a space sterilization method.

  Conventionally, floating bacteria are sterilized by spraying hypochlorous acid water into the space for the purpose of sterilizing bacteria floating in the air and for the purpose of deodorizing the space, and methyl mercaptan and toilet odor are deodorized. It is known to be used, and devices for spraying into space for the purpose of deodorizing are also sold.

By the way, there is no effect on sterilization and deodorization if the concentration sprayed in the indoor space for sterilization and deodorization is low, and if too high, acid gas is full and metal products in the space rust and health May be adversely affected.
For example, it is disclosed that 25 to 30 ml of hypochlorous acid water at a concentration of 50 to 150 ppm is sprayed on methyl mercaptan in a sealed space of 8.1 m ³ to obtain a deodorizing effect, but concentration by location The concentration in the space that is effective for sterilization and deodorization due to variations and inactivation is unknown.

JP, 2009-034025, A JP, 2009-178640, A

Proceedings of the 38th Annual Meeting of the Air Conditioning and Hygiene Engineering Association Kinki Branch Academic Research Conference, pp. 33-36

  The problem of the embodiment of the present invention is to define a hypochlorous acid concentration which is not adversely affected by hypochlorous acid water and which can sufficiently sterilize and deodorize the interior of the treated space, and maintain the concentration It is to provide a space sterilization method that can

According to an embodiment of the present invention, there is provided a space sterilization method in which the gaseous and / or atomized hypochlorous acid water is supplied to a space to be treated to sterilize the space.
In relation to the sterilization treatment time y (hour) and the unit area, the supply amount of the gaseous and / or atomized hypochlorous acid water supplied per unit time x (μg / (m ² · min) The space sterilization method which satisfies following formula (1) is provided.
y ≧ 16.217x− ^1.384 (1)

It is a figure showing an example of hypochlorous acid phase diagram. It is a schematic diagram showing an example of a structure of the space sterilizer of embodiment. It is a schematic diagram showing an example of the hypochlorous acid water supply part which can be used for embodiment. It is a schematic diagram showing an example of the sterilizer concerning embodiment. It is a graph showing the relationship between the theoretical space spray amount and the amount of effective hypochlorous acid. It is a graph showing the relationship between the theoretical space spray amount and the amount of effective hypochlorous acid. It is a graph showing the relationship between the supplied hypochlorous acid concentration and space hypochlorous acid concentration. It is a photograph showing the bactericidal effect of common bacteria of okra by the culture method. It is a photograph showing the bactericidal effect of common bacteria of okra by the culture method. It is a photograph showing the bactericidal effect of common bacteria of okra by the culture method. It is a photograph showing the bactericidal effect of common bacteria of okra by the culture method. It is a figure showing an example of the electrolysis device for producing acidity electrolysis water applicable to an embodiment. It is a graph showing the relationship between the amount of space of effective hypochlorous acid, and the amount of gas decomposition. It is a graph showing an example of the relation between dry mist supply and sterilization time. It is a graph showing the other example of the relationship between dry mist supply and sterilization time.

The space sterilizing apparatus according to the first embodiment includes a hypochlorous acid water supply unit that supplies gaseous and / or atomized hypochlorous acid water to a treated space, and the hypochlorite in the treated space The acid concentration can be 400 ppb to 500 ppm.
In addition, the space deodorizing apparatus according to the second embodiment includes a hypochlorous acid water supply unit that supplies gaseous and / or misty hypochlorous acid water to the to-be-treated space; The concentration of hypochlorous acid can be 400 ppb to 500 ppm.

  According to the embodiment, the hypochlorous acid water supply unit supplies gaseous and / or atomized hypochlorous acid water to the processing space, and the hypochlorous acid concentration in the processing space is 400 ppb to 500 ppm. By doing so, there is no adverse effect of hypochlorous acid water, and a sufficient sterilization and deodorizing effect can be obtained.

Here, the sterilization means to kill at least a part of the bacteria in the space to be treated.
Further, deodorizing means suppressing the growth of bacteria and preventing the generation or the emission of an odor.
Hypochlorous acid water, hypochlorous acid molecule (HClO) and hypochlorous acid ions (ClO ^-) and includes in equilibrium with, HClO is ClO ^- has a strong sterilizing power compared to. Component ratio of the two changes by pH, ClO in an alkaline region ^- the higher the ratio of, for the greater the HClO in weakly acidic region, can exhibit a strong sterilizing power. Incidentally, germicidal chlorine gas (Cl ²⁾ is, ClO ^- lower than sterilizing power.

FIG. 1 shows an example of a hypochlorous acid phase diagram at a temperature of 25 ° C., for example.
In the figure, the electrolyzed water is, depending on the pH, strongly acidic electrolyzed water in the SA region, weakly acidic electrolyzed water in the WA region, slightly acidic electrolyzed water in the MA region, and electrolyzed subaqueous water in the AI region.
The hypochlorous acid water supply unit can include a vaporizer that gasifies hypochlorous acid water, or a spray device that atomizes it.

Moreover, the space sterilization apparatus which concerns on embodiment can further include the system which measures hypochlorous acid density | concentration.
In one embodiment, a system for measuring the concentration of hypochlorous acid includes, for example, a container for collecting gaseous and / or atomized hypochlorous acid water in a processing space, and the container housed in the container; A hypochlorous acid water collecting part containing a fluorescent reagent which emits light by reacting with chloric acid and a luminescence intensity measuring part can be calculated, and the hypochlorous acid concentration can be calculated from the luminescence intensity.

As a fluorescent reagent to be used, an aminophenyl fluorescein (APF) reagent can be mentioned. The APF reagent is highly reactive to hypochlorous acid and produces a fluorescent compound by oxidation.
In another embodiment, a system for measuring the concentration of hypochlorous acid includes, for example, hypochlorous acid water having a container for collecting gaseous and / or atomized hypochlorous acid water in the space to be treated. The extraction unit may include a hypochlorous acid water extraction unit containing pure water contained in the container, and an effective chlorine concentration meter that measures an effective chlorine concentration in the pure water collected from the container.

Hypochlorous acid in the recovered pure water is colored by reaction using iodine absorptiometry or DPD method as the effective chlorine concentration meter to be used, and the effective chlorine concentration is measured, and the hypochlorous acid is measured based on the effective chlorine concentration. The chloric acid concentration can be calculated.
The hypochlorous acid concentration can preferably be 500 ppb to 200 ppm.

If it is less than 500 ppb, a sufficient bactericidal effect can not be obtained, and if it exceeds 200 ppm, the metal of the device or space tends to rust easily.
The space sterilizer according to the embodiment is also connected to an air suction device for suctioning air in the processing space, and the air suction device and the hypochlorous acid water supply unit, and the air introduced by the air suction device is in the form of gas and / or It may further include a sterilizing unit for sterilizing using misty hypochlorous acid water.

The schematic diagram showing an example of a structure of the space sterilizer provided with FIG. 2 with the suction device is shown.
As shown, this space sterilization device is connected to the suction unit 51, the hypochlorous acid water supply unit 56, and the sterilization unit 54 and the sterilization unit 54 connected to the suction unit 51 and the hypochlorous acid water supply unit 56. Exhaust portion 57 included. In addition, a dust removal filter 52 for removing coarse dust can optionally be provided in the front stage of the intake device 51. The intake device 51 includes an intake duct 58 and an intake fan 53. In the sterilizing part 54, the air introduced from the treated space into the sterilizing part 54 by the suction device 51 is disinfected using gaseous and / or misty hypochlorous acid water, and is exhausted again through the exhaust part 57 to the treated space. It can be discharged. The hypochlorous acid concentration in the sterilization unit 54 can be measured by an effective chlorine meter, an APF reagent or the like, and can preferably be 500 ppb to 200 ppm. If it is less than 500 ppb, sufficient sterilizing effect can not be obtained, and if it exceeds 200 ppm, metal corrosion in the device tends to be promoted by metal oxidization due to corrosive gas generated by equilibrium etc., and the device life tends to be shortened.

An example of the hypochlorous acid water supply part which can be used for FIG. 3 at embodiment is shown.
As illustrated, the hypochlorous acid water supply unit 1 includes a tank 10, a pipe 11, a pump 12, a spray device 13, and a controller 14.
The tank 10 stores hypochlorous acid water which is an example of sterilizing water. The hypochlorous acid water is appropriately replenished by hand via a water supply port provided in the tank 10 or by a power source such as a pump via a water supply pipe connected to the tank 10.

  One end of the pipe 11 is connected to, for example, the bottom surface of the tank 10, and the other end is connected to the spray device 13. The pump 12 functions as a liquid feeding device that supplies hypochlorous acid water of the tank 10 to the spray device 13, and is provided in the pipe 11. The pump 12 can adjust the flow rate of the hypochlorous acid water to be sent to the spray device 13 by, for example, variable control of the rotational speed. The hypochlorous acid water may be sent from the tank 10 to the spray device 13 using water head pressure or the like. In this case, the flow rate of the hypochlorous acid water to be sent to the spray device 13 can be adjusted, for example, by providing the piping 11 with a solenoid valve whose opening degree is variable.

The spray device 13 includes a housing 15 having an intake port 15 a and an exhaust port 15 b, and a carburetor 16 and a fan 17 housed in the housing 15. In the example of FIG. 3, the pipe 11 extends inside the housing 15 and is connected to the carburetor 16.
The vaporizer 16 vaporizes the hypochlorous acid water supplied through the pipe 11 and discharges the sterilizing component to the space. At the same time, the vaporizer 16 also generates mist having a relatively small particle size. Moreover, as a system of vaporization which does not mainly generate the mist, for example, an ultrasonic system can be adopted. In this case, the vaporizer 16 vibrates the container for storing the hypochlorous acid water supplied through the pipe 11 and the hypochlorous acid water stored in the container by ultrasonic waves so that And an ultrasonic transducer for generating a mist of chloric acid water. In addition, as a method of vaporizing hypochlorous acid water by the vaporizer 16, a method of vaporizing (atomizing) hypochlorous acid water by discharging hypochlorous acid water from a nozzle having a fine hole, etc. May be adopted. Furthermore, there is a natural vaporization type that blows air to the vaporization filter with a fan or the like. In the case of space sterilization, it is preferable to sterilize the object without wetting it, so it is desirable that the amount of mist generated be small and the particle size be small.

  The fan 17 sends the vaporized hypochlorous acid water and mist generated by the vaporizer 16 to the outside of the housing 15. Specifically, as the fan 17 rotates, air is taken into the housing 15 from the intake port 15a, and this air is discharged from the exhaust port 15b together with the vaporized hypochlorous acid water and mist generated by the vaporizer 16 (Sprayed). By variable control of the rotation speed of the fan 17, the amount of vaporized hypochlorous acid water or the like sprayed to the outside of the housing 15 can be adjusted.

  The controller 14 includes, for example, a processor responsible for controlling the first sterilizing device 1, a memory storing various setting conditions and computer programs executed by the processor, and a power supply device generating a voltage to be supplied to each part. There is. The controller 14 controls the pump 12, the vaporizer 16, the fan 17 and the like. In the example of FIG. 3, the controller 14 is connected to an input / output device 18 provided with a display device such as a display lamp or a display, an input device such as a button or a switch, and an audio output device such as a speaker.

  Such vaporized sterilizing water (hypochlorous acid water) can be disinfected without wetting the inner wall of the space to be treated, and it evaporates quickly even if a mist having a small particle size is generated at the same time. There is no excessive wetting of the inner wall surface etc. of the processing space. Therefore, it is suitable for sterilization of the processing object unsuitable for washing with water.

As hypochlorous acid water, for example, electrolytic water obtained by electrolysis of an aqueous electrolyte solution containing sodium chloride, potassium chloride or inorganic chloride can be used.
The effective chlorine concentration in hypochlorous acid water is preferably 10 to 200 ppm.
Hypochlorous acid water preferably has a pH of 7 or less.

The space sterilizer of FIG. 2 can be used as a space deodorizer, and the hypochlorous acid water supply unit of FIG. 3 can be applied to the space deodorizer.
When used as a space deodorizing device, hypochlorous acid water preferably has a pH of 3 to 7.
Example 1
In Example 1, the space existing amount of hypochlorous acid was calculated using a fluorescent reagent as follows, and the bactericidal effect of common bacteria of okra was evaluated.

Calculation of Space Amount of Hypochlorous Acid The inventors first determined the space amount of hypochlorous acid due to the difference in pH and the difference in spray form as follows.
FIG. 4 is a schematic view showing a sterilizer applicable to the first embodiment.
First, since deactivation and the like are predicted, a sealed space 20 with a width of 3 m and a depth of 4 m and a height of 2 m was prepared for the experiment.

20 ml of a 5000-fold diluted solution of APF reagent manufactured by Sekisui Medical Co., Ltd. is put in each of the first, second and third petri dishes, and the first, second and third hypochlorous acid water collecting portions 22, 23, 24 It was created.
As shown, the space sterilizer 25 is provided in the enclosed space 20, and the hypochlorous acid water supply unit 1 having the same configuration as FIG. 3 and the hypochlorous acid water supply to the floor 21 of the enclosed space 20. The first hypochlorous acid water collecting unit 22, the second hypochlorous acid water collecting unit 23, and the third hypochlorous acid water collecting unit 24, which are arranged in order from the position close to the unit 1, And a spectrofluorometer.

  Hypochlorous acid water having an effective chlorine concentration of 50 mg / L obtained on the anode side by electrolysis of sodium chloride using a two-separator three-chamber type electrolyzed water generator was prepared at pH 3 and pH 6. The pH was adjusted by adding the electrolytic alkaline water obtained on the cathode side of the same device.

The obtained hypochlorous acid water was applied to, for example, the hypochlorous acid water supply unit of the sterilizing apparatus 25 and was sprayed in a closed space for 2 hours.
The actual spray amount of hypochlorous acid water was determined from the difference in weight of hypochlorous acid water before and after spraying.
The theoretical space spray amount per treatment time was determined from the space volume.

The fluorescence emission intensity in the petri dish after 30 minutes, 1 hour and 2 hours, respectively, was measured using a non-illustrated spectrofluorimeter. From the measurement results, the amount of hypochlorous acid water dropped onto the petri dish after 30 minutes, 1 hour and 2 hours was calculated.
Furthermore, after spraying, in order to confirm the presence or absence of hypochlorous acid water floating immediately above the petri dish, it was left at the same place for 2 hours. The sum of the amount of hypochlorous acid water during and after spraying is calculated as the amount of hypochlorous acid water reached on the volume of the cylinder immediately above the petri dish within the treatment time, and this is the amount of space of hypochlorous acid, ie It was the concentration of chlorous acid.

In addition, emission intensity was measured by excitation wavelength 490 nm and fluorescence wavelength 526 nm, using Shimadzu RF-5300PC as a spectrofluorimeter. In addition, a calibration curve was obtained by directly injecting hypochlorous acid water of a known concentration into a 5000-fold reagent of APF, and the luminescence intensity thereof.
FIG. 5 shows the relationship between the amount of theoretical space spray obtained from the weight change when spraying an aqueous solution of 50 ppm effective chlorine concentration of pH 3 with a vaporization type device and the amount of hypochlorous acid captured. FIG.

In the figure, the graph 31, the graph 32, and the graph 33 respectively indicate the first hypochlorous acid water collecting unit 22, the second hypochlorous acid water collecting unit 23, and the third hypochlorous acid water collecting unit. The measurement result in 24 petri dishes is shown. The graph 30 shows the theoretical total spray amount.
From FIG. 5, it can be seen that the effective hypochlorous acid space abundance is 20 to 30% of the theoretical space spray volume at pH 3.

Further, FIG. 6 is a graph showing the relationship between the theoretical spray amount obtained from the weight change when spraying 50 ppm hypochlorous acid water of pH 6 with a vaporization type device and the amount of hypochlorous acid water captured. Figure shows.
In the figure, the graph 41, the graph 42, and the graph 43 show the first hypochlorous acid water collecting portion 22, the second hypochlorous acid water collecting portion 23, and the third hypochlorous acid water collecting portion, respectively. The measurement result in 24 petri dishes is shown. Graph 40 shows the theoretical total spray amount.

It can be seen from FIG. 6 that the effective space presence of hypochlorous acid is 20 to 50% of the theoretical space spray amount at pH 6.
The bactericidal effect of common bacteria of okra The first hypochlorous acid water collecting part 22, the second hypochlorous acid water collecting part 23, and the third hypochlorous acid water collecting part 24 of the experimental sealed space 20 Each okra placed on a petri dish was placed in the vicinity.

  Hypochlorous acid water having an effective chlorine concentration of 50 mg / L is prepared at pH 3 and pH 6, and the space existing amount of effective hypochlorous acid is 0 ppb, 200 ppb, 400 ppb, 500 ppb, 5 ppm, 50 ppm, 200 ppm, 500 ppm The theoretical spray amount was set to become and each was exposed for 2 hours. In addition, a chlorine gas sensor (Toxire-2-CL2 manufactured by Nippon Toxire Systems, Inc.) was set in the space room for safety.

The effective space amount of hypochlorous acid in the booth can be adjusted in advance by setting the concentration of hypochlorous acid water, pH, the spray amount per unit time, and the like by experiment.
FIG. 7 is a graph showing the relationship between the supplied hypochlorous acid concentration and the spatial hypochlorous acid concentration.

Graphs 81 and 82 are space concentrations after 2 hours when hypochlorous acid of various concentrations of pH 3 and pH 6 is sprayed into the experimental space at 200 ml / hour, respectively.
For example, to bring 200 ppb into the experimental sealed space 20, hypochlorous acid water having a concentration of 130 mg / L for 2 hours at pH 3 and hypochlorous acid having a concentration of 100 mg / L for pH 6 Water can be sprayed continuously for 2 hours. Also, depending on the desired amount of space present, spraying can be performed at regular intervals, even if not continuous.

The common bacteria on the surface of the okra were collected with a cotton swab, transferred to a standard agar medium, and cultured in a 37 ° C. incubator for 24 hours.
Each of the above experiments was performed four times (N1 to N4).
FIGS. 8A to 8D are photographs showing the bactericidal effect of common bacteria of okra by the culture method.

According to the general bacterial count of okra, the effective growth of hypochlorous acid at 400 ppb or more showed the effect of preventing bacterial growth.
FIG. 8A shows the general bacterial counts of okra when the spatial abundance is 0 ppb.
Also, FIG. 8B shows the general bacterial counts of okra at 5 ppm, FIG. 8C at 50 ppm, and FIG. 8D at 200 ppm.

The chlorine gas monitor showed 0.5 ppm when the effective space chlorine content of hypochlorous acid exceeded 500 ppm.
Similarly, the experiment was carried out in pH 3 hypochlorous acid water. The results are shown in Table 1 below.

これにおいても４００ｐｐｂ以上で殺菌効果がみられた。
なお、図９に、実施例１に使用可能な２隔壁３室型の電解水生成装置の一例を表す概略図を示す。
図示するように、この電解水生成装置１５０は、陽極室１５４と、陰極室１５５と、陽極室１５４及び陰極室１５５の間に設けられた中間室１５１との３室からなる３室型電解槽１５８を有する。中間室１５１は、無機塩化物を含む電解質水溶液として飽和食塩水１６５とその無機塩化物である残留塩１６６とを収容する飽和食塩水貯留器１６１、塩水循環ポンプ１６２、及び食塩水供給ライン１６９を備えた飽和食塩水循環システム１６３と接続され、常にほぼ飽和状態の食塩水１６５が供給される。一方、陽極室１５４及び陰極室１５５は、給水システム１６４と接続され、各々、常に新しい水が供給される。中間室１５１と陽極室１５４の間は陰イオン交換膜１５２で仕切られ、中間室１５１と陰極室１５５の間は陽イオン交換膜１５３で仕切られている。陽極室１５４には陽極電極１５６、陰極室１５５には陰極電極１５７が備えられており、それぞれにプラスとマイナスの電圧が印加されている。

Also in this case, the bactericidal effect was observed at 400 ppb or more.
In addition, the schematic showing an example of the 2 partition 3 chamber type electrolyzed water generating apparatus which can be used for Example 1 is shown.
As shown, this electrolyzed water generating device 150 is a three-chamber electrolytic cell consisting of an anode chamber 154, a cathode chamber 155, and an intermediate chamber 151 provided between the anode chamber 154 and the cathode chamber 155. It has 158. The intermediate chamber 151 has a saturated saline solution reservoir 161 containing a saturated saline solution 165 and the residual salt 166 which is the inorganic chloride solution as an electrolyte aqueous solution containing inorganic chloride, a saline circulation pump 162, and a saline solution supply line 169. It is connected with the provided saturated saline circulation system 163, and a nearly saturated saline solution 165 is always supplied. On the other hand, the anode chamber 154 and the cathode chamber 155 are connected to the water supply system 164, and fresh water is always supplied. The intermediate chamber 151 and the anode chamber 154 are partitioned by an anion exchange membrane 152, and the intermediate chamber 151 and the cathode chamber 155 are partitioned by a cation exchange membrane 153. The anode chamber 154 is provided with an anode electrode 156, and the cathode chamber 155 is provided with a cathode electrode 157, to which positive and negative voltages are respectively applied.

  In the anode chamber 154, chlorine ions in the intermediate chamber 151 are pulled by the anode electrode 156, pass through the anion exchange membrane 152, move to the anode chamber 154, pass electrons at the anode electrode 156, become chlorine gas, The reaction produces hypochlorous acid and hydrochloric acid. Acidic electrolyzed water containing hypochlorous acid and hydrochloric acid is taken out through an acid electrolyzed water line 167 together with chlorine gas which can not be dissolved in the acid electrolyzed water.

  In the cathode chamber 155, sodium ions in the intermediate chamber 151 are pulled by the cathode electrode 157, pass through the cation exchange membrane 153, move to the cathode chamber 155, and hydrogen ions decomposed by water at the cathode electrode 157 receive electrons. Hydrogen gas to produce sodium hydroxide. The aqueous solution of sodium hydroxide is taken out as an alkaline electrolyzed water through an alkaline electrolyzed water line 171.

In addition, sodium chloride, potassium chloride etc. are mention | raise | lifted as an inorganic chloride used for embodiment, In this case, sodium hydroxide aqueous solution and potassium hydroxide aqueous solution are respectively obtained as alkaline electrolyzed water.
Example 2
In Example 2, the space existing amount of hypochlorous acid was calculated using a reagent other than the fluorescent reagent, and the gas decomposition performance of methyl mercaptan was evaluated as a deodorizing effect.

First, prepare 1 m ³ of booth for experiment.
In this experimental booth, a sonic sprayer as a hypochlorous acid water supply unit, a hypochlorous acid water collection unit such as a petri dish, and a space deodorizing apparatus including an effective chlorine concentration meter are provided.
For example, a fixed amount of 100 ppm / N 2 of methyl mercaptan gas was injected into the experimental booth, and the methyl mercaptan concentration in the booth was set to 3 ppm. Furthermore, an ultrasonic sprayer AreaClean CS-P102 manufactured by CGI Corporation was installed in the booth, and hypochlorous acid water was sprayed. The decomposition amount of methyl mercaptan gas was measured by the space concentration of hypochlorous acid water.

  The concentration of methyl mercaptan gas was measured using a gas detector gas collector GV-110, and the gas detector tube No. It measured by 70 L (mercaptans detection tube). Space concentration put 20 ml of water on the petri dish, take out the petri dish after a certain time, measure the effective chlorine concentration of the water by iodine reagent absorptiometry using AQUAB AQ-102 made by Shibata Chemical Co., Ltd. The hypochlorous acid concentration was determined.

A graph showing the relationship between the gas decomposition amount (initial gas concentration-gas remaining amount) of methyl mercaptan with respect to the space existing amount of effective hypochlorous acid is shown in FIG.
In the figure, 83 represents the methyl mercaptan decomposition performance at pH 3, 84 at pH 6.
As shown, it can be seen that the gas is decomposed in proportion to the concentration of HClO in the space, and the ratio is 1: 1.

In Example 2, the space existing amount of hypochlorous acid was calculated using an iodine reagent, and the gas decomposition performance of methyl mercaptan was evaluated. However, as shown in Example 1, the same evaluation is performed using a fluorescent reagent. Is also possible.
Example 3
The surface of the target placed in the germicidal space is disinfected, but its germicidal activity is defined by the germicidal concentration of the germicidal space, the sterilizing time, the germicidal object (bacteria type and number (density), etc.).

FIG. 11 shows the relationship between the bactericidal concentration and the required bactericidal treatment time required for the concentration when the Botrytis cinerea fungus which has been implanted on the surface of okra is the object of sterilization.
The horizontal axis of the graph 90 represents hypochlorous acid dry mist supply amount μg / (m ² · min) supplied per unit time per unit area. The amount of hypochlorous acid dry mist supplied can be calculated by a fluorescence intensity measurement method using a fluorescent reagent for active oxygen detection (Aminophenyl Fluorescein), commonly called APF solution.

Here, after installing the dry mist of hypochlorous acid water of a predetermined concentration in the spray space for a certain period of time, the petition per unit area and unit time is obtained from the amount of HCLO in the petri dish recovered Calculate the amount of hypochlorous acid dry mist supplied to the product.
On the other hand, the vertical axis indicates the sterilization treatment time, and indicates the treatment time required to sufficiently sterilize the target for any dry mist supply amount. For example, in the spray space where hypochlorous acid dry mist is 53 μg / (m ² · min) using hypochlorous acid water at a concentration of 100 (mg / l) as condition (1), 1.3 hours It shows that if it is treated (80 minutes), the object can be sterilized. When sterilization was actually performed under condition (1), the number of Botrytis cinerea fungi which had been 3 million on the surface of the okra decreased to 7,000, and it was confirmed that there was no onset of the fungus even after storage at 20 ° C for one week thereafter There is. On the other hand, in samples without sterilization treatment, the onset of Botrytis cinerea is observed after one week under the same storage conditions.

Further, assuming that the surface area of the okra is 113 cm ² , the value obtained by multiplying the dry mist supply amount by the surface area and the treatment time is the total supply amount of dry mist sprayed on the surface of one okra. Hereinafter, the dry mist total supply amount (μg) is referred to.
The total supply amount of dry mist supplied to one okra in the case of sterilization treatment under the condition (1) is 48 μg.

In addition, under the condition (2), using a hypochlorous acid water having a concentration of 25 mg / L, the dry mist supply amount is 7 μg / (m ² · min), and the sterilization time is 24 hours, equivalent to the condition (1) In order to obtain the bactericidal effect, a total supply amount of 112 μg and a total dry mist supply amount of twice or more are required. In FIG. 11, there is a bactericidal effect in the area 91 above the graph 90. That is, the bactericidal effect can not be judged only by the simple dry mist total supply amount, and it turns out that the bactericidal concentration supplied instantaneously influences.

Further, it is said that the gray mold fungus of the present example is a fungus, and bacteria represented by E. coli and the like can be killed with a 1/10 bactericidal activity of the fungus, so a spray space having a bactericidal effect is defined in FIG. Do.
From the above results, in the relationship between the sterilization treatment time y (hours) and the hypochlorous acid dry mist supply amount x (μg / (m ² · min), y = 16.217x represented by the graph 92 in FIG. ^-1.384 or more area 93, that is, the following formula (1)
y ≧ 16.217x− ^1.384 (1)
The spray space has a bactericidal effect.

The invention described in the claims at the beginning of the present application is appended below.
[1] A hypochlorous acid water supply unit for supplying gaseous and / or atomized hypochlorous acid water to a space to be treated, wherein the concentration of hypochlorous acid in the space to be treated is 400 ppb to 500 ppm Space sterilizer.
[2] The space sterilizer according to [1], wherein the hypochlorous acid water supply unit includes a vaporizer that gasifies hypochlorous acid water, or a spray device that atomizes it.
[3] The space sterilizer according to [1] or [2], further including a system for measuring the hypochlorous acid concentration in the processing space.
[4] The system for measuring the concentration of hypochlorous acid in the space to be treated includes a container for collecting the gaseous and / or misty hypochlorous acid water in the space to be treated, and the container Containing a hypochlorous acid water collecting part containing a fluorescent reagent which is contained and reacts with the hypochlorous acid and emits light, and a luminescence intensity measuring part, and the hypochlorous acid concentration is calculated from luminescence intensity [3] Space sterilization device as described in.
[5] The space sterilizer according to [4], wherein the fluorescent reagent is an aminophenyl fluorescein reagent.
[6] The system for measuring the concentration of hypochlorous acid in the space to be treated comprises a container for collecting the gaseous and / or misty hypochlorous acid water in the space to be treated. An acid water collecting unit, a hypochlorous acid water collecting unit containing pure water contained in the container, and an effective chlorine concentration meter for measuring an effective chlorine concentration in the pure water collected from the container [3] Space sterilization device as described in.
[7] The available chlorine concentration meter measures the concentration of available chlorine by causing hypochlorous acid in the recovered pure water to develop color by reaction using iodine absorptiometry or DPD method, and based on the available chlorine concentration, The space sterilizer according to [6], which calculates hypochlorous acid concentration.
[8] The space sterilizer according to any one of [1] to [7], wherein the hypochlorous acid concentration in the space to be treated is 500 ppb to 200 ppm.
[9] The space sterilizer according to any one of [1] to [7], wherein the pH in the hypochlorous acid water is 7 or less.
[10] An intake device for taking in air in the processing space; and the intake device and the hypochlorous acid water supply unit connected to the intake device, the air introduced by the intake device is in the form of gas and / or mist The space sterilizer according to any one of [1] to [9], comprising a sterilizer that sterilizes using hypochlorous acid water.
[11] The relationship between the sterilization treatment time y (hour) and the unit area and the amount of hypochlorous acid dry mist supplied per unit time x (μg / (m ² · min) is expressed by the following equation (1) The space sterilizer according to any one of [1] to [10], which is satisfied.
y ≧ 16.217x− ^1.384 (1)
[12] A hypochlorous acid water supply unit for supplying gaseous and / or atomized hypochlorous acid water to a space to be treated, wherein the concentration of hypochlorous acid in the space to be treated is 400 ppb to 500 ppm Space deodorizer.
[13] The space deodorizing apparatus according to [12], wherein the hypochlorous acid water supply unit includes a vaporizer that gasifies hypochlorous acid water, or a spray device that atomizes it.
[14] The space deodorizing apparatus according to [12] or [13], further including a system for measuring the hypochlorous acid concentration.
[15] The system for measuring the hypochlorous acid concentration comprises a container for collecting the gaseous and / or atomized hypochlorous acid water in the processing space, and the container for accommodating the container in the container, The space deodorizing method according to [14], comprising a hypochlorous acid water collecting unit containing a fluorescent reagent that emits light by reacting with chloric acid, and a luminescence intensity measuring unit, and calculating the hypochlorous acid concentration from the luminescence intensity apparatus.
[16] The space deodorizing device according to [15], wherein the fluorescent reagent is an aminophenyl fluorescein reagent.
[17] The system for measuring the concentration of hypochlorous acid comprises a hypochlorous acid water collecting portion having a container for collecting the gaseous and / or atomized hypochlorous acid water in the space to be treated; The space deodorization according to [14], including a hypochlorous acid water collecting unit containing pure water contained in a container, and an effective chlorine concentration meter for measuring an effective chlorine concentration in the pure water collected from the container apparatus.
[18] The available chlorine concentration meter measures the concentration of available chlorine by causing hypochlorous acid in the recovered pure water to develop color by reaction using iodine absorptiometry or DPD method, and based on the available chlorine concentration, The space deodorizing apparatus according to [17], which calculates the concentration of hypochlorous acid.
[19] The space deodorizing device according to any one of [12] to [18], wherein the hypochlorous acid concentration is 1 to 200 ppm.
[20] The space deodorizing apparatus according to any one of [12] to [18], wherein the pH in the hypochlorous acid water is 3 to 7.
[21] An intake device for taking in air in the processing space; and the intake device and the hypochlorous acid water supply unit connected to the intake device, the air introduced by the intake device is in the form of gas and / or mist The space deodorizing apparatus according to any one of [12] to [20], comprising a sterilizing unit that sterilizes using hypochlorous acid water.
[22] The relationship between the sterilization treatment time y (hour) and the unit area and the amount of hypochlorous acid dry mist supplied per unit time x (μg / (m ² · min) is expressed by the following equation (1) The space deodorizing device according to any one of [12] to [21], which is satisfied.
y ≧ 16.217x− ^1.384 (1)
While certain embodiments of the present invention have been described, these embodiments have been presented by way of example only, and are not intended to limit the scope of the invention. These novel embodiments can be implemented in various other forms, and various omissions, substitutions, and modifications can be made without departing from the scope of the invention. These embodiments and modifications thereof are included in the scope and the gist of the invention, and are included in the invention described in the claims and the equivalent scope thereof.

  20 ... enclosed space, 22, 23, 24 ... hypochlorous acid water collecting part, 25 ... space sterilizer

Claims (11)

A space sterilization method for supplying gaseous and / or atomized hypochlorous acid water to a space to be treated and performing sterilization,
In relation to the sterilization treatment time y (hour) and the unit area, the supply amount of the gaseous and / or atomized hypochlorous acid water supplied per unit time x (μg / (m ² · min) , Space sterilization method to satisfy the following formula (1).
y ≧ 16.217x− ^1.384 (1)   The hypochlorous acid water collecting portion having a container for collecting the gaseous and / or atomized hypochlorous acid water in the processing space, the supplied amount of hypochlorous acid water collected in the container The space sterilization method according to claim 1, wherein the measurement system for measuring the concentration of hypochlorous acid in the to-be-treated space from the above satisfies the formula (1) when it is measured.   The space sterilization method according to claim 2, wherein when the hypochlorous acid concentration is measured by the measuring system, the hypochlorous acid water concentration in the processing space is 400 ppb to 500 ppm.   The measurement system further includes a light emission intensity measurement unit, the fluorescent reagent that emits light in response to hypochlorous acid is contained in the container, and the light emission intensity is measured by the light emission intensity measurement unit, and the obtained light emission The space sterilization method according to claim 2 or 3 which computes said hypochlorous acid concentration from intensity.   The space sterilization method according to claim 4, wherein the fluorescent reagent is an aminophenyl fluorescein reagent.   The measurement system further includes an effective chlorine concentration meter, containing pure water in the container, measuring an effective chlorine concentration in pure water collected from the container with the effective chlorine concentration meter, The space sterilization method according to claim 2 or 3 which computes hypochlorous acid concentration.   The available chlorine concentration meter measures the concentration of available chlorine by causing hypochlorous acid in the recovered pure water to develop color by reaction using iodine absorptiometry or DPD method, and based on the available chlorine concentration, hypochlorite The space sterilization method of Claim 6 which calculates acid concentration.   8. The hypochlorous acid water supply unit according to any one of claims 1 to 7, wherein the hypochlorous acid water supply is performed using a hypochlorous acid water supply unit including a vaporizer which gasifies hypochlorous acid water or a sprayer which atomizes it. The space sterilization method according to any one of the above.   The space sterilization method according to any one of claims 1 to 8, wherein the hypochlorous acid concentration in the space to be treated is 500 ppb to 200 ppm.   The space sterilization method according to any one of claims 1 to 9, wherein the pH in the hypochlorous acid water is 7 or less.   The space sterilizer is connected to an air suction device for suctioning air in the processing space, the air suction device and the hypochlorous acid water supply unit, and the air introduced by the air suction device is in the form of gas and / or The space sterilization method according to any one of claims 1 to 10, further comprising a sterilizing unit that sterilizes using a mist of hypochlorous acid water.

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