JP2019047824A - Barley leaf and/or stem cultivated using andosols, aso-grown barley leaf and/or stem and edible composition including the same, and method for cultivating barley stem and/or leaf - Google Patents

Abstract

To provide: six-rowed barley leaves and/or stems suitable for an edible composition; the edible composition excellent in cultivation aptitude, nutrition and palatability due to inclusion of such six-rowed barley leaves and/or stems; the six-rowed barley leaves and/or stems, taste and nutrition value of which are improved because of raw material itself; and a method for cultivating the barley stems and/or leaves, by which the barley stems and/or leaves are grown in a short period to be capable of efficiently manufacturing the barley stems and/or leaves so that taste and nutrition value is improved.SOLUTION: This invention relates to: six-rowed barley leaves and/or stems used for an edible composition, which are cultivated using soil in which at least a part of the soil is andosols; six-rowed barley leaves and/or stems cultivated in Aso district, Kumamoto prefecture; and a method for cultivating barley stems and/or leaves, the method comprising performing cultivation in a condition that a period when a daily range of month average is 10°C or more lasts for three months or more.SELECTED DRAWING: None

Description

The present invention is cultivated using a soil containing at least a part of Andosol, and leaves and / or stems of six-row barley used in a composition for eating and drinking, and a composition for eating and drinking comprising them; Barley leaves and / or stems grown in the Aso area, and food and drink compositions containing the same; barley leaves and / or grown under conditions in which the daily difference on a monthly average of 10 ° C. or more lasts 3 months or more The present invention relates to a stalk and a food and drink composition containing the stalk.

The composition for eating and drinking for green juice is processed into a dry powder, a squeezed powder and the like including plant leaves and stems. The composition for eating and drinking for green juice is used as a health food because it can easily ingest vegetable components.

Barley is mentioned as one of the plants used for the food-drinks composition for green juice. Barley is native to Central Asia and is an annual or overennial herb belonging to the family Poaceae. Barley's young leaves are said to be rich in vitamins and minerals, amino acids, dietary fiber, chlorophyll and SOD enzymes. Barley is classified into two-row barley and six-row barley according to the ear shape. Two-rowed barley and six-rowed barley differ in the number of rows of fruits attached to their ears, and when viewed from the top of the spike, two-rowed barley has two rows and six-rowed barley has six rows. Six-row barley is said to have been delivered to Japan in the second and third centuries via Korea, and in addition to being used as grain, it is also used as a raw material for barley tea. On the other hand, Nijo barley is said to be introduced to Japan from the West in the Meiji period, and is mainly used for brewing.

As a method of cultivating barley fruits, most of them are planted by subversion or change of paddy fields, but in some areas such as Kanto, they are planted in the fields. In particular, in the flourishing northern Kanto region where barley is cultivated, barley is planted in a field consisting of Ando soil, which is a soil containing a large amount of humus with a volcanic ash soil as a base material (see Non-Patent Document 1).

Moreover, with the background of rising popularity of green juice as a health food, green juice that is more nutritious and delicious and easy to drink is required by consumers. For example, in patent document 1, green leaf powder and / or green leaf juice contains white jellyfish powder, thereby providing green juice excellent in taste, aroma, and throat penetration without losing nutrients. In Patent Document 2, tea leaves, mulberry leaves and young barley leaves are finely pulverized, and brown sugar powder, honey powder and granulated sugar are added to provide a powdery food which is easy to drink and has a good flavor and color tone.

In addition, in Patent Document 3, water culture is carried out at a nighttime minimum temperature of less than 25 ° C. and a diurnal difference with the daytime air temperature of 3 ° C. or more under a photoperiod of 10 to 15 hours during the above-ground growth period. It is disclosed that by doing so, a large number of tubers can be produced efficiently. Patent Document 4 discloses a method of processing young barley leaves to improve palatability.

JP, 2006-166776, A JP, 2005-210972, A JP 2004-73214 A Unexamined-Japanese-Patent No. 5-7471

Tonooka Takuji and 4 others, "Improvement of barley sorrel quality in Ando soil-Usefulness of starch variation trait that presents powdery endosperm-" Journal of The Japan Crop Science Society, 2010, Vol. 79, No. 3, p. 296-307

However, as described in Non-Patent Document 1, when barley is planted in a soil of the Andosol, the protein content of the grain is higher than in the case of planting in a gray lowland soil paddy field, whereby the proportion of vitreous grains is increased. , The barley quality is significantly degraded. Therefore, it is said that it is preferable to plant barley in a paddy field when obtaining high-quality refined barley.

On the other hand, in order to harvest barley leaves and stems, there is no known document that describes the fields on which barley should be planted. In particular, no literature is known which describes how six-row barley leaves and stems suitable for food and drink compositions for green juice can be obtained.

In addition, inventions have not been made yet on efficient cultivation of young barley leaves and methods for achieving both taste and nutritional value.

Therefore, the object of the present invention is to provide a six-row barley leaf and / or a stem suitable for a food and drink composition such as green juice, and further to include such a six-row barley leaf and / or a stem. An object of the present invention is to provide a food composition which is highly suitable, rich in nutrition and highly palatable.

Further, the subject of the present invention is to improve the taste and nutritional value of the raw material itself rather than to improve the taste and maintain or improve the nutritional value of the food and drink such as green juice by the additive. And / or providing a stem.

Furthermore, the subject of the present invention is to grow barley stems and / or leaves which can grow large in a short period of time, can efficiently produce barley stems and / or leaves, and can enhance taste and nutritional value. To provide a way.

The inventors of the present invention have studied earnestly the above-mentioned problems, and surprisingly, according to the cultivation method using Ando soil which has been said to cause poor barley, the leaves and stems of six-row barley are the conventional cultivation methods. Compared to the leaves and stems of six-row barley obtained by using it, it is suitable as a raw material for food and drink compositions such as green juice because it has high cultivation suitability, is rich in nutrition, is beautiful in appearance, and has a good flavor. The composition for food and drink using this was found to be rich in nutrition and highly palatable. In addition, by cultivating six-row barley in the Aso area of Kumamoto Prefecture, the growth of stems and leaves of barley is good, the content of specific nutrients is significantly improved, and the nutrients affect the taste. I found it. Furthermore, it is found that, by cultivating under conditions that a daily difference of 10 ° C. or more lasts three months or more on a monthly average, the stems and leaves of barley can be greatly grown in a short time, and at the same time the taste and nutritional value can be enhanced. The The present invention has been completed based on these findings.

The present invention is summarized as follows.
[1] Six-row barley leaves and / or stalks grown using soil containing at least a part of Andosol.
[2] The leaves and / or stems of six-row barley which are cultivated using a soil containing at least a part of the soil and the soil is used in a composition for food and drink.
[3] At least one property selected from the group consisting of length, split, color, stem diameter and leaf width is superior to those grown using red soil, and used for a composition for eating and drinking The six-rowed barley leaves and / or stems.
[4] A method of cultivating the leaves and / or stems of six-row barley used in the composition for eating and drinking, comprising the step of cultivating the seeds or seedlings of six-row barley using soil containing at least a part of the soil.
[5] A food and drink comprising the six-row barley leaf and / or stem according to any one of the above [1] to [3], or the six-row barley leaf and / or stem obtained by the method according to [4] Composition.
[6] The leaves and / or stems of six-row barley grown in the Aso area of Kumamoto Prefecture.
[7] A composition for eating and drinking comprising the barley leaf and / or the stem according to the above [6].
[8] A method for cultivating barley stalks and / or leaves, which is grown under conditions in which a daily difference of 10 ° C. or more on a monthly average lasts 3 months or more.
[9] The method according to the above [8], wherein the cultivation period is from sowing to heading before heading.
[10] A composition for eating and drinking comprising the stems and / or leaves of barley grown by the method described in [8] or [9] above.
[11] A stem and / or a leaf of barley grown under conditions that a daily average of 10 ° C. or more lasts three months or more on a monthly average.
[12] A method of Andokoku soil farming, characterized by cultivating six-row barley using soil which contains at least a part of the soil.
[13] Aso caldera farming method characterized by cultivating six-row barley in Aso area, Kumamoto.

The six-row barley leaves and / or stems of the present invention are more suitable for cultivation, rich in nutrients, beautiful in appearance, and tasted compared to the six-row barley leaves and stems obtained using conventional cultivation methods. It is good and suitable for food and drink compositions, particularly for food and drink compositions for green juice. The composition for eating and drinking of the present invention obtained by using the leaves and / or stems of the six-row barley of the present invention is rich in nutrition and highly palatable.

According to the method and agricultural method of the present invention, barley can be grown quickly and barley young leaves can be efficiently produced, and at the same time, taste and nutritional value can be enhanced.

It is a photograph figure which photographed Andok soil. It is a photograph figure which photographed red soil.

Hereinafter, the details of the present invention will be described.

[1. Stems and leaves of six-row barley grown using soil containing the Andosol of the present invention]
Six-rowed barley is a botanically classified as six-rowed barley, that is, six-rowed barley (Hordeum vulgare f. Hexastichon) of the gramineous barley genus Barley, and as a morphological feature, the ear is viewed from above No particular limitation is imposed as long as the fruit is found in the sixth column, and any of wild species, hybrids, etc. may be used. As a specific example of the six-row barley in the present invention, a composition for eating and drinking, more specifically, a composition for eating and drinking for green juice can be mentioned. The composition for food and drink using six-row barley according to the present invention as a raw material has a high level of cultivation suitability, content of nutrient components, and bright color compared to a conventional composition for food and drink using two-row barley leaves and stems. It is possible to provide the beauty of appearance and the goodness of flavor. The varieties of six-row barley include double-dressed, silky snow, fiber snow, minnow wheat, oystergrass, ssu cold, red pepper power (registered trademark), early mater, sunray, sanuki hatakan, daishochi, ichiban boshi, haikumagi and kashimamagoru etc. However, the present invention is not limited thereto.

In the present invention, the six-rowed barley leaf and / or the stem may be a six-rowed barley leaf, a stem or both, and the leaf and the stem may each be a part or all thereof. In the present specification, a leaf and / or a stem may be referred to as a stem and leaf.

In the present invention, although not particularly limited, it is preferable to cultivate and harvest barley from sowing to heading before heading in order to enhance the taste and nutritive value of barley stems and leaves, and it is more preferable to cultivate and harvest just before heading preferable.

The shoots and leaves of the six-row barley according to the present invention are cultivated using a soil containing at least a part of Andosol. In the present invention, cultivation is not particularly limited as long as six-row barley can be grown to the extent that leaves and stems can be harvested from seeds and seedlings by a method generally known in the art. Conditions such as humidity can be appropriately set by those skilled in the art.

In the present invention, black and white soil is not particularly limited as long as it is generally known and black and white soil, for example, it mainly consists of volcanic ash soil and mulch, has a color close to black, and feels smooth (lightly It is known that the soil is very smooth (see Figure 1). Compared to red soil (see FIG. 2) it can be seen that the black color is darker. Moreover, compared with red soil, Andosols soil has a small content of effective phosphoric acid, and the N: P ratio is about 2 to 5 to 12 to 17. Therefore, when barley is grown on Andok soil, excess phosphorus can be avoided for barley. Furthermore, the amount of cation exchange is about twice as high as that of red soil. As a result, compared with red soil, Andosan soil tends to contain rich amounts of exchangeable lime, exchangeable magnesia and exchangeable potassium. A non-limiting example of soil analysis data for Andosol is as shown in Table 1 below. For reference, soil analysis data of red soil is also shown.

In the present invention, the soil containing at least a part of Andosol is not particularly limited as long as Andoro soil or a mixed soil obtained by mixing Andoro soil and another soil. For example, in the case of the mixed soil, the mixing ratio of the black and white soil and the other soil can be made black and white soil: other soil = 0.1 to 9.9: 9.9 to 0.1. Is 1 to 9: 9. When grown in mixed soil, by increasing the proportion of Andok soil, the length, division, color, stem diameter, and leaf width of six-row barley can be greater than that grown in soil different from Andok soil, such as red soil. Or any two or more of these properties.

The length, division, color, stem diameter and leaf width of six-row barley are characteristics that affect the quality of the raw material of the food and drink composition. The larger the height, stem diameter and leaf width of the six-rowed barley, and the greater the number of tillers, the better the growing condition and the better the cultivation suitability. The color of the six-rowed barley is preferably dark green because it affects the aesthetics of the food and drink composition used as an ingredient. Specifically, when a leaf color scale is used, the average value of one barley leaf is, for example, 4.0 or more, preferably 4.5 or more.

Six-rowed barley grown using soil containing at least a part of Andosol, for example, when the cultivation conditions and cultivation days other than the soil used are the same as those grown in red soil, the length, It is not particularly limited as long as at least one property selected from the group consisting of color, stem diameter and leaf width is good as a raw material of the composition for food and drink, and preferably one of these properties is cultivated with red soil 1.1 times or more as compared to those obtained, more preferably 1.2 times or more as compared with those grown in red soil, more preferably those one property grown in red soil It is 1.3 times or more compared to the one or 1.1 times or more compared to those grown in red soil. For example, when the leaf width of barley grown in red soil is 5 cm and the leaf width of barley grown in Andok soil is 6 cm, the leaf width of barley grown in Andok soil is the same as that grown in red soil. It is 1.2 times more than that.

In addition, six-row barley grown using soil that contains at least a part of the black and white soil, for example, when the cultivation conditions other than the soil used and the number of cultivation days are the same as those grown in red soil, The total polyphenol content and the amino acid content tend to be high. Therefore, it is preferable that the shoots and leaves of the six-row barley according to the present invention have higher total polyphenol content, total amino acid content, or both total polyphenol content and amino acid content as compared with those grown in red soil.

The stems and leaves of six-row barley according to the present invention are used in a composition for food and drink. When the stems and leaves of six-row barley according to the present invention are used as a raw material of the composition for food and drink, one specific variety can be used alone, or can be used in combination of two or more varieties together with other varieties.

The stems and leaves of the six-row barley according to the present invention are higher in cultivation suitability, rich in nutrition, beautiful in appearance, and have a better flavor than the six-row barley leaves and stems obtained by using the conventional cultivation method. And are suitable as raw materials for food and drink compositions. Therefore, the composition for eating and drinking using the stems and leaves of six-row barley according to the present invention is rich in nutrition and has a color, for example, compared to the composition for eating and drinking using red soil as a raw material It is highly palatable with the beauty of appearance and the good taste due to its vividness. In particular, when the composition for food and drink including the stems and leaves of six-row barley according to the present invention is in the form of a powder, the green color becomes bright when it is mixed with water. Further, the flavor of the composition for food and drink of the present invention will be specifically described. The composition for food and drink of the present invention is more vivid than the conventional composition for food and drink despite the fact that the green color is bright as described above. Sweet, strong and weak. In addition, the composition for food and drink of the present invention is weak in taste, weak in greenish odor, and can be ingested deliciously, as compared with the composition for food and drink in the related art.

It is preferable that the stems and leaves of barley be harvested before the ripening period, that is, from the beginning of the tilling period to the early period of the onset of heading. Specifically, although it varies depending on the difference in breed, it is generally preferred to harvest the shoots from barley having a height of 10 cm or more, particularly about 10 to 90 cm, especially about 30 to 60 cm, but is limited thereto It is not a thing. Barley stems and leaves are preferably processed immediately after harvest. If it takes time to treat, it is stored by storage means commonly used by those skilled in the art, such as low temperature storage, to prevent deterioration of barley stems and leaves.

Barley stems and leaves can be processed products that have been subjected to various types of processing in order to be used as raw materials for food and drink compositions. As such processed products, for example, dry powder of stem and leaf, cut and dried powder of stem and leaf, squeezed and dried powder of stem and leaf, extract of stem and leaf, dry powder thereof, and the like can be mentioned.

For example, conventionally known methods can be used to dry and powderize barley stems and leaves. As such a method, a method in which drying treatment and grinding treatment are combined can be used for barley stems and leaves. Either of the drying process and the pulverizing process may be performed first, but it is preferable to perform the drying process first. In the dry pulverization, one or more treatments selected from treatments such as blanching treatment, sterilization treatment and the like may be further combined with this method, if necessary. In addition, although the number of times of pulverizing treatment may be one, or two or more treatments may be combined, it is preferable to combine the pulverizing treatment of carrying out the coarse pulverizing treatment and then pulverizing more finely.

The blanching treatment is a treatment for keeping the green color of the stems and leaves bright, and examples of the blanching treatment include a hot water treatment and a steaming treatment. In the blanching treatment, preferably, the shoots are treated for 60 to 180 seconds, preferably for 90 to 120 seconds, in hot water or steam at 80 to 100 ° C, preferably 90 to 100 ° C. In addition, when hot water treatment is performed as blanching treatment, the green color of the stems and leaves can be made more vivid by dissolving carbonate such as magnesium carbonate or hydrogen carbonate such as sodium hydrogen carbonate in hot water. It is preferable because it can be done. In addition, as the steaming treatment, it is preferable to perform intermittent steaming treatment while repeating the treatment of steaming stems and leaves and the treatment of cooling under normal pressure or under pressure. In the intermittent steaming process, the steaming process is preferably performed for 20 to 40 seconds, more preferably for 30 seconds. It is preferable that the cooling treatment after the steaming treatment be performed immediately, and the method is not particularly limited, but immersion in cold water, refrigeration, cold air cooling, evaporative cooling with warm air, evaporative cooling combined hot air and cold air Etc. are used. Among these, vaporization cooling in which warm air and cold air are combined is preferable. Such cooling treatment is performed such that the material temperature of the shoots is preferably 60 ° C. or less, more preferably 50 ° C. or less, and most preferably 40 ° C. or less. Moreover, in order to manufacture the powder of the leaves and leaves rich in nutritional components such as vitamins, minerals, and chlorophyll, it is preferable to repeat intermittent steaming treatment 2 to 5 times.

Moreover, sterilization treatment is treatment which kills a microbial cell physically and chemically using temperature, pressure, electromagnetic waves, medicine etc. normally. When the blanching treatment is performed in addition to the drying treatment and the pulverizing treatment, the blanching treatment is preferably performed before the drying treatment. When sterilization treatment is performed in addition to the drying treatment and the grinding treatment, the sterilization treatment is preferably performed after the drying treatment, or before or after the grinding treatment.

Moreover, it is preferable that it is a process which is dried so that the moisture content of a stem and leaf may be 10% or less, especially 5% or less as a drying process. This drying process may be performed by any method known to those skilled in the art, such as hot air drying, high pressure steam drying, electromagnetic wave drying, lyophilization and the like. Drying by heating may be carried out at a temperature and for a time such that the stems and leaves are not discolored by heating, preferably at 40 ° C to 140 ° C, more preferably 80 to 130 ° C.

In addition, examples of the grinding treatment include grinding with a crusher, a mill, a blender, a mill, etc. by any method commonly used by those skilled in the art. The ground stems and leaves are optionally sieved, and it is preferable to use, for example, one which passes 30 to 250 mesh as a powder of stems and leaves. By setting the particle size to 250 mesh or less, it is easy to handle at the time of further processing of foliage powder, and by making the particle diameter to be 30 mesh or more, uniform mixing of foliage powder with other materials Is easy.

As a specific method of dry powderization, for example, after cutting the stems and leaves of barley, blanching treatment is performed, and then dried so that the water content is 10% by mass or less, preferably 5% by mass or less, and then The method to grind | pulverize is mentioned (refer Unexamined-Japanese-Patent No. 2004-000210). For example, after cutting the stems and leaves of barley, a blanching treatment is performed, and then it is twisted, dried, and then crushed and crushed (see JP-A-2002-065204 and JP-B-3428956). For example, after drying and roughly crushing barley stems and leaves, a method of heating at 110 ° C. or more and further pulverizing may be mentioned (see JP 2003-033151 A and JP 3277181 A).

As a method of shredding barley stems and leaves, methods commonly used by those skilled in the art when shredding plants, such as slicing, crushing, shredding, etc., can be used. Slurry may be used as an example of fragmentation. Slurrying is carried out by subjecting barley stems and leaves to a mixer, juicer, blender, mascaroder or the like to make the barley stems and leaves into a scaly curd (a liquid and solid suspension). By slurrying in this way, the stems and leaves preferably have an average diameter of at least 1 mm, more preferably at most 0.5 mm, more preferably at most 0.1 mm, most preferably at most 0.05 mm, of 80% by mass or more of the fragments. It becomes finely divided and becomes fluid.

As a method of squeezing barley stems and leaves, there may be mentioned a method of squeezing barley stems and leaves or fragments thereof, or a method of centrifuging or filtering the fragments of barley stems and leaves. A typical example is a method of obtaining a squeezed juice by squeezing with a mechanical crusher such as a mixer or juicer, and removing crude solid content by a method such as sieving or filtration if necessary. . Specifically, the methods described in JP-A-08-245408, JP-A-09-047252, JP-A-5-7471, JP-A-4-341153 and the like can be mentioned, and these known methods can be mentioned. Can be selected as appropriate by those skilled in the art.

In addition, as a method of obtaining an extract of barley stems and leaves, an extraction solvent generally used by those skilled in the art such as ethanol, water, hydrous ethanol and the like is added to the stems and leaves of barley or a fragment thereof, and stirring or heating is performed as necessary. Can be mentioned. The extract may be concentrated if necessary.

In the present invention, it is possible to make the composition for food and drink more vivid in color and have a favorable flavor in the present invention that using dried powder of stems and leaves among the processed products of stems and leaves of the above-mentioned six-row barley It is preferable from the point which can be made abundant.

In another aspect of the present invention, there is provided a method of cultivating the stems and leaves of six-row barley of the present invention. The method of the present invention includes the step of cultivating the seeds or seedlings of six-row barley using soil containing at least a part of the soil containing the Andosol. According to still another aspect of the present invention, there is provided an Andosan agricultural method characterized by cultivating six-row barley using a soil containing at least a part of the Andosan soil. In the method and agricultural method of the present invention, any step may be employed before or after the above steps, as long as the purpose of cultivating the stems and leaves of six-row barley of the present invention is not impaired.

In the solid content of the food and drink composition, the content of stems and leaves of barley is a dry mass, and the lower limit is preferably 0.1 mass% or more, more preferably 0.5 mass% or more, and 1 mass% or more More preferably, 10% by mass or more is particularly preferable, and most preferably 20% by mass or more, and the upper limit thereof is preferably 99.9% by mass or less, more preferably 90% by mass or less, and particularly preferably 80% by mass or less . When the stem and leaf content of the six-row barley of the present invention is less than 0.1% by mass, the effects of the present invention may not be sufficiently exhibited.

As another aspect of the present invention, there is provided a food and drink composition, preferably a composition for green juice, comprising the shoots and leaves of six-row barley according to the present invention or the shoots and leaves of six-row barley obtained by the method of the present invention. The form of the food and drink composition of the present invention is not particularly limited, and can be any form. The form of the composition for food and drink of the present invention is, for example, a form suitable for oral intake such as food and drink, specifically, powder, granular, granular, tablet, rod, plate, block, solid Round, liquid, scaly, paste, cream, capsule-like like hard capsule or soft capsule, caplet-like, tablet-like, gel-like, jelly-like, gummi-like, wafer-like, biscuit-like, cookie-like, cake-like , Chewable, syrupy, stick-like and the like.

As a specific example of the composition for food and drink of the present invention, various beverages such as soft drinks, various foods such as bread and confectionery, and noodles, cooked products and the like can be mentioned. The beverages referred to here are green juice, green juice and juice, vegetables and dairy products added to make juice, shakes, smoothies, soft drinks, carbonated drinks and their original forms etc. It can be mentioned. The beverage referred to here includes not only a liquid composition but also a solid composition which is mixed with a solvent such as water at the time of drinking to form a liquid beverage. Moreover, as bread and confectionery, breads such as bread, confectionery bread, French bread, British bread, muffins, steamed bread, donuts, waffles and the like, butter cakes, sponge cakes, chiffon cakes, cakes such as hot cakes, sherbet , Ice, and other frozen desserts, jelly, cookies and the like. Examples of noodles include udon and noodles. Examples of cooked products include soups such as curry, stew, miso soup, and vegetable soup, as well as powdered seasonings and the like.

The composition for food and drink according to the present invention is in the form of powder (in the form of powder such as powder and granules) and is orally ingested in a mixture mixed with water to prevent rot and is suitable for long-term storage. It is preferable because the color is clear when the composition for use is mixed with water. When the composition for food and drink of the present invention is in the form of a solid, as described above, it may be a liquid which is mixed with water, and the liquid may be taken orally, such as by drinking it, Depending on their preference etc., they may be taken orally as solid. In addition to water, it may be used by adding to milk, soy milk, fruit juice beverages, whey beverages, soft drinks, yogurt, hot cake mixes and the like. Needless to say, it may be used as a nutrition function indication food or a food for specified health use.

[2. Stem and leaf of six-row barley grown in the Kumamoto prefecture Aso region of the present invention]
The stems and leaves of six-row barley used in the present invention are characterized in that they are cultivated in the Aso area of Kumamoto Prefecture. According to the weather statistics information of the Japan Meteorological Agency website, the weather in the Aso area in 2012 (for example, Takamori) is an average annual precipitation of 240 mm / month, an average annual temperature of 12.8 ° C, and an average annual sunshine time of 135 hours / month. .

On the other hand, the climate (2012) of Hokkaido (for example, Obihiro) where a lot of barley is cultivated is annual average precipitation 98.1 mm / month, annual average temperature 7.2 ° C, annual average sunshine time 157 hours / month The climate (2012) of Saga Prefecture (for example, Tosu) which is growing a lot of barley in the same way is the average annual precipitation 190 mm / month, and the climate of Tosu and neighboring Kurume is the annual average precipitation 238 9 mm / month, average annual temperature 12.8 ° C, annual average sunshine time 135 hours / month.

Thus, the climate has large regional differences and affects the growth of barley, which in turn affects the amount of nutrients contained in the stems and leaves of barley. When growth of barley is affected, for example, cultivation suitability such as length, tillering, color, leaf width, stem diameter, leaf length, leaf thickness, etc. may change. As described in 1 above, it is the characteristics that influence the quality of the raw material of the composition for eating and drinking, the length, division, color, stem diameter and leaf width of six-row barley.

Six-rowed barley grown in the Aso area, for example, is selected from the group consisting of length, tillage, color, stem diameter and leaf width when the cultivation conditions and cultivation days are the same as those grown in the bird's-eye area The composition is not particularly limited as long as at least one of the properties is good as a raw material of the composition for food and drink, and preferably one or more of these properties are 1.1 times or more as compared with those grown in the bird's eye area Preferably, one of these properties is at least 1.2 times greater than that grown in the area of the bird's-eye area, and more preferably one or more times that of one of these properties is greater than that grown in the area of the bird's area. The two characteristics of are 1.1 times more than those grown in the bird area. For example, when the leaf width of barley grown in the bird area is 5 cm and the leaf width of barley grown in the Aso area is 6 cm, the leaf width of barley grown in the Aso area is the one grown in the bird area It is 1.2 times more than that. In addition, as for the said comparison, it is preferable to use the thing of 15 days or more of cultivation days.

Nutrients contained in six-row barley grown in the Aso area are not particularly limited, and examples thereof include lipids, carbohydrates, dietary fibers, various vitamins, folic acid, inorganic substances such as calcium, magnesium and potassium, polyphenols, chlorophyll, various amino acids and the like, specifically, proteins, lipids, carbohydrates, dietary fiber, sodium, vitamin a, vitamin _{B 1,} vitamin _{B 2,} vitamin _{B 6,} vitamin _{B 12,} vitamin C, vitamin E, vitamin K ₁ , biotin, pantothenic acid, carotene, folic acid, niacin, calcium, magnesium, potassium, phosphorus, zinc, copper, iron, manganese, chromium, iodine, aspartic acid, alanine, arginine, isoleucine, glycine, glutamic acid, cysteine, threonine , Tyrosine, tryptophan, valine, fe Nylalanine, proline, methionine, lysine, leucine, total amino acid, octacosanol, catechin, gluconic acid, polyphenol, chlorophyll, lutein, γ-aminobutyric acid, β-glucan and the like. Moreover, SOD like activity shall also be included here.

As a nutrient contained significantly in the stems and leaves of six-row barley according to the present invention, for example, amino acids can be mentioned, preferably asparagine, glutamine, aspartic acid, glutamic acid. Some of these amino acids are also umami ingredients and are thought to affect taste. In addition, it was found that when the same six-rowed barley was grown in Hokkaido and Aso, respectively, these amino acids were significantly more contained in the stems and leaves of six-rowed barley grown in Aso.

Also, six-row barley grown in the Aso area tends to have a higher total amino acid content and total chlorophyll amount when the cultivation conditions and the number of cultivation days are the same, for example, as compared with those grown in the bird's-eye area. Therefore, the stems and leaves of six-row barley in the present invention are higher in total amino acid content, total chlorophyll content, or all two of them in about 31 days after the start of cultivation as compared with those cultivated in the bird's eye area Is preferred. In addition, it is more preferable that the stems and leaves of six-row barley in the present invention are not all lower in total amino acid amount and total chlorophyll amount in about 31 days after the start of cultivation as compared with those cultivated in the bird area.

As another aspect of the present invention, an Aso caldera farming method is provided, which is characterized by cultivating six-row barley in the Aso area of Kumamoto Prefecture. In the agricultural method of the present invention, any step may be adopted before or after the above-described step, as long as the object of cultivating the stems and leaves of six-row barley of the present invention is not impaired.

The stems and leaves of six-row barley according to the present invention can be processed into a composition for eating and drinking such as green juice. When using the stems and leaves of six-row barley according to the present invention as a raw material of a composition for eating and drinking, one particular variety of six-row barley can be used alone, or used in combination of two or more varieties with other varieties. Can. In addition to the processing method for the composition for food and drink, the characteristics of the six-row barley, and the form of the additive and the composition for food and drink which may be blended as a composition for food and drink, are described above [1. Reference can be made to the corresponding description in the “Stalks and Leaves of Six-row Barley Grown Using Soil Containing Andorax Soil of the Present Invention”. A preferred embodiment of the present invention is a stem and leaf of six-row barley which is cultivated using soil containing at least a part of Andosol in the Aso area of Kumamoto Prefecture.

[3. The cultivation method of the stems and leaves of barley which are grown under the condition that the daily difference of 10 ° C. or more lasts three months or more on a monthly average according to the present invention]
Six-row barley is as described in 1 above, two-row barley is used to put the fruits on the panicle in two rows, and examples of two-row barley include Nishinohoshi, Haruka Nijo, Nishinochikara, and Hashi-zushi. Although any barley may be used in the present invention, six-row barley is preferred.

In the method of cultivating barley leaves of the present invention, the cultivation is conducted under the condition that a daily difference of 10 ° C. or more lasts three months or more on a monthly average. If the difference is less than 10 ° C, the growth of plant height after sowing is poor. Preferably, the diurnal difference is 10 ° C. to 20 ° C., more preferably 10 ° C. to 15 ° C., still more preferably 10 ° C. to 13 ° C. on a monthly basis. The diurnal difference is the difference (the temperature difference) between the maximum temperature and the minimum temperature of the day.

It is preferable that the average temperature of the day is at least 4 ° C. or more, because the stems and leaves of barley hardly grow when the temperature is less than 4 ° C. In addition, the average daily temperature is preferably 30 ° C. or lower, because even if the temperature is too high, it is not suitable for cultivating barley.

It is preferable that a daily average of 10 ° C. or more on a monthly basis is stably continued until the barley is harvested, preferably until young barley leaves are harvested. In order to harvest young barley leaves, it is generally grown for 20 to 90 days, it is required that there is an average daily difference of 10 ° C. or more during that time. In addition, it is preferable to grow in a period in which the daily difference of 10 ° C. or more lasts 3 months or more on a monthly average, and the daily difference of 10 ° C. or more on a monthly average is 3 months or more, preferably 6 months or more, more preferably 9 months or more It can be harvested repeatedly if it is grown in a place where it will continue.

In the above-mentioned diurnal difference, barley may be grown by selecting such a field under natural conditions, or barley may be grown in a field such as a green house where such conditions can be made artificially.

Cultivation conditions other than the above daily range, for example, cultivation temperature, photoperiod, drainage, seed preparation, soil improvement, soil pH, fertilization, cultivation / soiling, sowing, weeding, treading / draining, pest control etc. However, for example, “2. Wheat cultivation standard” of “Ministry of Agriculture, Forestry and Fisheries” “Measures for cultivating rice, wheat and soybeans”, and “Growth and utilization of barley” Reference can be made to "Terban" and the like.

The above-mentioned diurnal variation affects the growth of barley, and as a result, the amount of nutrients contained in the stems and leaves of barley. When growth of barley is affected, for example, cultivation suitability such as length, tillering, color, leaf width, stem diameter, leaf length, leaf thickness, etc. may change. As described in 1 above, barley length, tiller, color, stem diameter and leaf width are properties that affect the quality of the raw material of the composition for eating and drinking.

For example, barley grown under conditions where the daily difference of 10 ° C. or more lasts 3 months or more lasts, for example, compared to that grown under conditions where the daily difference of 10 ° C. or more is less than 3 months When the number of days of cultivation is the same, it is not particularly limited as long as at least one property selected from the group consisting of length, division, color, stem diameter and leaf width is good as a raw material of the composition for food and drink. Preferably, one of these properties is 1.1 times or more, more preferably, one of these properties is 1.2 times or more, more preferably, one of these properties is 1.3 times or more or these two properties are 1.1 times or more. For example, the leaf width of barley grown under conditions in which the daily difference of 10 ° C. or more is less than 3 months on a monthly average is 5 cm, and the daily difference of 10 ° C. or more on a monthly average is grown under conditions continuing for 3 months or more When the leaf width of barley is 6 cm, the leaf width of barley grown under the condition that the daily difference of 10 ° C or more continues for 3 months or more on a monthly average is less than 3 months on a monthly average of 10 ° C or more It is more than 1.2 times more than that grown under conditions. In addition, as for the said comparison, it is preferable to use the thing of 15 days or more of cultivation days.

According to the cultivation method of the present invention, the plant height becomes 30 cm or more in about one month after sowing, and the growth of barley can be promoted. In addition, the content of carbohydrates, proteins, lipids, vitamins, five major nutrients of minerals, pigments unique to plants, organic compounds, etc. which are components contained in the stems and leaves of barley harvested by the cultivation method of the present invention is improved. it is conceivable that. Specifically, proteins, lipids, carbohydrates, dietary fiber, sodium, vitamin A, vitamin _{B 1,} vitamin _{B 2,} vitamin _{B 6,} vitamin _{B 12,} vitamin C, vitamin E, vitamin _{K 1,} biotin, pantothenic acid , Carotene, folic acid, niacin, calcium, magnesium, potassium, phosphorus, zinc, copper, iron, manganese, chromium, iodine, aspartic acid, alanine, arginine, isoleucine, glycine, glutamic acid, cysteine, threonine, serine, tyrosine, tryptophan, Valine, phenylalanine, proline, methionine, lysine, leucine, total amino acid, octacosanol, catechin, gluconic acid, polyphenol, chlorophyll, lutein, γ-aminobutyric acid, β-glucan and the like. Furthermore, it is considered that the SOD-like activity (superoxide scavenging activity) is also improved.

Examples of nutrients significantly contained in the leaves and leaves of barley cultivated by the method of the present invention include amino acids, preferably asparagine, glutamine, aspartic acid, serine, glutamic acid, histidine, glycine, threonine, valine and isoleucine. It is. Some of these amino acids are also umami ingredients and are thought to affect taste. In addition, when the same six-row barley is grown in areas with different daily differences, these amino acids are significantly more significant to the stems and leaves of barley grown under conditions where daily differences of 10 ° C. or more last more than three months on a monthly average. It turned out that a lot is included.

In addition, barley grown under conditions in which the daily difference of 10 ° C. or more lasts three months or more on a monthly average is, for example, one grown under conditions in which the daily difference on 10 ° C. or more is less than 3 months. On the other hand, when the cultivation days are the same, the total amount of amino acids and the total amount of chlorophylls tend to be high. Therefore, the stems and leaves of barley obtained by the cultivation method of the present invention are about 31 days after the start of cultivation, compared to those cultivated under a condition that the daily difference at 10 ° C. or more is less than 3 months on a monthly average, It is preferred that the total amino acid content, the total chlorophyll content or all two of them be high. In addition, the stems and leaves of barley obtained by the cultivation method according to the present invention are those grown about 31 days after the start of cultivation compared with those cultivated under a condition that the daily difference is 10 ° C or more on average monthly. It is more preferred that the total amount of amino acids and the total amount of chlorophyll not be low.

The stems and leaves of six-row barley according to the present invention can be processed into a composition for eating and drinking such as green juice. When using the stems and leaves of six-row barley according to the present invention as a raw material of a composition for eating and drinking, one particular variety of six-row barley can be used alone, or used in combination of two or more varieties with other varieties. Can. In addition to the processing method for the composition for food and drink, the characteristics of the six-row barley, and the form of the additive and the composition for food and drink which may be blended as a composition for food and drink, are described above [1. Reference can be made to the corresponding description in the “Stalks and Leaves of Six-row Barley Grown Using Soil Containing Andorax Soil of the Present Invention”. A preferred embodiment of the present invention is a stem and leaf of six-row barley grown in the Aso area of Kumamoto Prefecture under the condition that a daily difference of 10 ° C. or more on a monthly average lasts three months or more. Another preferred embodiment of the present invention is a foliage of six-rowed barley cultivated using a soil containing at least a part of Andok soil under conditions in which a daily difference of 10 ° C. or more on a monthly average lasts three months or more. A more preferable embodiment of the present invention is a six-row barley grown in the Aso area of Kumamoto Prefecture under a condition that a daily difference of 10 ° C. or more lasts three months or more on a monthly average. Stem and leaf.

Hereinafter, the present invention will be described in more detail by way of examples, but the present invention is not limited to these examples.

[1. Comparative Evaluation of Barley Grown Using Andosols, Red Soils or Their Mixed Soils]
1. Cultivation planters using Andosoku soil put black and white soil, and for each planter, 6 varieties of barley "Silky Snow", "Shunrai", "Fiber Snow" and "Kashimagor" or 4 varieties of Nijo barley "Nishinohoshi" and "Haruka" Seeds (20 g / m ² ) of 4.8 g of three varieties of Nijo and Rokujo barley "Ichiban Boshi" were sown. The above six varieties of barley were grown by ordinary plant cultivation methods such as water supply and weed management. The “Kashemagol” and “Haruka Nijo” species used were provided by the Kyushu Okinawa Agricultural Research Center of the National Agriculture and Food Research Institute.

2. Barley was grown in the same manner as in 1 above, except that a mixed soil of black and white soil and red soil (black soil: red soil = 1: 9) was used instead of the grown soil using the mixed soil. In addition, red soil was dried by sun-drying, and used what crushed the solidified part.

3. Cultivation using red soil Barley was cultivated in the same manner as in 1 above except using red soil. In addition, red soil was dried by sun-drying, and used what crushed the solidified part.

About the cultivation condition of said 1-3, what summarized the use kind, soil, and cultivation time was made into Table 2.

4. Evaluation example 1
About six rows of barley under cultivation, at intervals of 10 days, length (length), number of tillers, stem diameter (thickness), leaf width (width) and color (use the scale, the larger the value, the better the color tone) It was measured. Table 3 shows the relative values of Andosol and mixed soil based on the measured value of red soil. As shown in Table 3, six-row barley grown in Ando soil or a mixed soil of Andoro soil and red soil has the same length, number of tillers, stem diameter, in the same number of cultivation days as compared with those grown in red soil. It was excellent in any of the characteristics of leaf width and color, or two or more of these characteristics. In addition, it germinated on the 3rd day after the cultivation start under the condition using the black and white soil, but germinated around 7th day after the cultivation start on the condition using the red soil and the mixed soil. From this, it was found that the use of Andok soil can shorten the number of germination days.

Similarly, with respect to six-rowed barley under cultivation, characteristics such as length, number of tillers, color, leaf width and stem diameter were measured at 5, 11, 17 and 21 days after sowing. Table 4 shows the measured values of Andosols and red soil and the relative values of Andros based on the measured values of red soil. As shown in Table 4, six-row barley grown in Andosol has characteristics of any of length, number of tillers, stem diameter, leaf width and color in the same cultivation days as compared with those grown in red soil Or it was excellent in these 2 or more types of characteristics. However, even with the same six-row barley, this tendency was weak for Ichibanboshi. From these results, it was found that cultivation in Andosoku soil can enhance the growth of fiber snow, silky snow and shunrai than cultivation in red soil.

※ 表中、「Ｎ．Ｄ．」はデータがないことを示す。

※ In the table, “ND” indicates that there is no data.

5. Evaluation example 2
The stems and leaves of six-row barley "Silky snow" and "Kashimagol" and Nijo barley "Nishinohoshi" and "Haruka Nijo" harvested 33 days after the start of cultivation are washed with water to remove adhering mud and the like, and 1 g of the obtained stem and leaf The total polyphenol content (% by mass) was measured using chlorogenic acid as a standard substance according to the Folin-Denis method (comment published by the Japan Food Analysis Center, 5th edition of the Japanese Food Standard Composition Table Analysis Manual). The results are shown in Table 5. As Table 5 shows, in the case of six-row barley "Silky snow" and "Kashemagol", the total polyphenol content tended to increase when grown on Ando soil and mixed soil compared to when grown on red soil . On the other hand, such a tendency was not seen in Nijo barley "Nishinohoshi" and "Haruka Nijo". This indicates that cultivation in a soil containing at least a part of Ando-buro soil results in a stem and leaf of six-row barley having a relatively high total polyphenol content.

6. Evaluation example 3
After the stems and leaves harvested 33 days after the start of cultivation are washed with water to remove attached mud and the like, the obtained stems and leaves are squeezed using a juicer, and the obtained squeezed solution is used to carry out an automatic precolumn using HPLC. The amino acid content was measured by the derivatization method. As a result, with regard to several amino acids, similar to the total polyphenol content, in the case of six-row barley, the amino acid content tended to increase when grown on Ando soil and mixed soil compared to when grown on red soil . This indicates that when grown in soil containing at least a part of Ando-buro soil, stems and leaves of six-row barley having a relatively high amino acid content can be obtained.

[2. Temperature difference comparison of Aso area and Tosu area]
The Aso area and the Tosu area were selected as areas to grow barley, and on October 2, 2013, barley was sown and cultivated in each field. In this specification, the Aso area refers to a region that exhibits a similar climate in the area around Aso mountain including Kikuchi in Kumamoto Prefecture, and the Tosu region refers to Kurisu in Tosu in Saga Prefecture and Kurume in Fukuoka Prefecture. An area that exhibits a similar climate throughout the flat land area.

About air temperature and temperature difference of cultivation period, data of October 1, 2013 to December 31, 2013 in Aso area (Kikuchi) and Tosu area (Kurume) are shown in Table 6 and Table 7 (from Meteorological Agency website). Moreover, what summarized the average temperature and temperature difference of October-December 2013 for every month is shown in Table 8.

From Tables 6 to 8, it was found that the daily range (temperature difference) for three months from October to December in the Aso area is continuously 10 ° C. or more. On the other hand, the daily range (temperature difference) for the three months from October to December in the Tosu region was less than 10 ° C.

Moreover, the temperature data (from the Meteorological Agency website, measurement period 1981 to 2010) of Aso area (Kikuchi) and Tosu area (Kurume) are shown in Table 9 below.

According to Table 9, in the Aso area, the daily difference (temperature difference) is 10 ° C or more on the yearly basis, specifically 11.3 ° C on the yearly average, and for 9 months from September to May, the daily difference is monthly It was found that the average temperature was 10 ° C. or higher. In the Tosu region, the diurnal difference is less than 10 ° C on average, specifically 9.3 ° C on average, and the diurnal difference is 10 ° C or more on a monthly basis for two months from April to May. all right.

[3. Growth comparison evaluation of barley grown in Aso area and Tosu area (1)]
In autumn 2013, Nijohoshi of Nijo barley, fiber snow of Nijo barley, Silky snownow and Sunrai in Nijo barley are cultivated in the fields of Aso area and Tosu area, and after sowing in Aso area on days 8, 19, and 29, On the 39th, 44th, 51st and 63rd days, in the Tosu area on the 8th, 15th, 27th, 34th, 41st, 49th and 63rd days after sowing , Plant height was measured. The results are shown in Table 10 below.

From Table 10, when the plant height was compared in the days after similar sowing in the Aso area and the Tosu area, it was found that the growth when grown in the Aso area was good in any of the varieties. Furthermore, in the Aso area, all barley varieties tested became 40 cm or more on the 39th day after sowing, and there was a clear difference in growth in about one month as compared to those grown in the Tosu area.

In addition, when the plant height on the 39th day after sowing in the Aso area is compared with the plant height on the 41st day after sowing in the Tosu area, the cultivation period in the Aso area is shorter by 2 days, but It turned out that one grows more than the bird's-eye area.

Furthermore, Table 11 shows the results of comparing the days after sowing in which the height exceeds 45 cm in cultivation in the fall of 2013.

As shown in Table 11, when grown in the Aso area, the number of days in which the height exceeds 45 cm was shorter in any of the cultivars as compared with the bird's-eye area, indicating that the growth was good.

From the above, barley grown under conditions where a daily difference of 10 ° C. or more lasts three months or more on a monthly average is improved in growth as compared to barley grown under conditions not satisfying such conditions, It has been found that barley stems and / or leaves can be grown efficiently.

[4. Comparative growth evaluation of barley grown in Aso area and Tosu area (2)]
In the field of Aso area and Tosu area in the fall of 2014, six-row barley fiber snow, silky snow, shunrai and ichiban boshi are grown and plant height is 5, 5, 17 and 21 days after sowing in each variety The characteristics such as length), number of pieces, color (scale was used, the larger the value, the better the color tone), leaf width (width) and stem diameter (thickness) were measured. Table 12 shows the measured values of Aso area-cultivated barley and Tosu region-grown barley and the relative values of Aso area-grown barley based on the measured values of Tosu region-cultivated barley. As shown in Table 12, among the six-rowed barley grown in the Aso area, fiber snow, silky snow and sunrai are superior in the above-mentioned characteristics to the whole in the same number of cultivation days as those grown in the bird area It was However, even with the same six-row barley, this tendency was weak for Ichibanboshi. From these results, it was found that cultivation in the Aso area can enhance the growth of fiber snow, silky snow and sun ray rather than cultivation in the tori area.

※ 表中、「Ｎ．Ｄ．」はデータがないことを示す。

※ In the table, “ND” indicates that there is no data.

[5. Comparative evaluation of plant height of barley grown in Aso area and Hokkaido area]
In spring 2013, in Hokkaido and Aso, Nijohoshi of Nijo barley, fiber snow of Six-row barley, Silky snow and Shiunrai were grown, and the plant height was measured about 60 days after sowing.
The results are shown in Table 13. The unit of numerical values in the table is "cm".

[6. Comparative evaluation of plant height of barley grown in Aso area, Tosu area and Hokkaido area]
In the spring of 2013, in the fields of Aso area, Tosu area and Hokkaido area, Nijohoshi of Nijo barley, Fibresnow of six-row barley, Silky snownow and Sunnrai are grown, and the number of days after sowing for height exceeds 45 cm in each variety The results are shown in Table 14.

As shown in Table 14, when grown in the Aso region, the number of days in which the height exceeds 45 cm was short in all the varieties, as compared with the bird's-eye region and the Hokkaido region, indicating that the growth was good.

[7. Comparative evaluation of amino acid content of barley grown in Aso area, Tosu area and Hokkaido area]
In the spring of 2013, in the Aso area, the Tosu area and the Hokkaido area, the six-row barley fiber snow, silky snow and sun ray and the two-row barley nishinohoshi were cultivated for about 70 days from sowing, and the leaves were harvested. This was cut into small pieces, and the small pieces were squeezed to obtain a squeezed solution.

The content of amino acid per 100 g of stem and leaf juice was measured by an automatic pre-column derivatization method using HPLC. In addition, 500 μL of 0.1 N HCl was added to 500 ml of the above stem and leaf squeezed solution, mixed well, centrifuged at 15,000 rpm for 10 minutes, and the supernatant was filtered with a 0.45 μm membrane filter to obtain a sample solution. The results are shown in Table 15.

In any cultivar, in general, those grown in Aso region clearly have higher contents of asparagine, glutamine, aspartic acid and glutamic acid in stems and leaves as compared with those grown in Hokkaido region and Tosu region I understood. In addition, the content of six-row barley (fiber snow, silky snow, sun ray) tended to be further improved compared to the two-row barley (nishinohoshi).

[8. Comparative Evaluation of Amino Acid Content of Barley Grown in Aso Area and Tosu Area]
In the fall of 2013, in the Aso and Tosu regions, the stems and leaves grown and harvested for about 70 days after sowing Nishinohoshi Nijo barley and Six-row barley fiber sock, silky snow and sun ray are used as in 7 above, The total content of amino acids per 100 g of dry powder of stems and leaves was measured by an automatic pre-column derivatization method using HPLC. The results are shown in Table 16.

In any cultivar, when grown in the Aso area, the contents of asparagine, glutamine, aspartic acid, serine, glutamic acid, histidine, glycine, threonine, valine and isoleucine are evident in the leaves compared to those grown in the Tosu area It turned out to be high. In addition, the content of six-row barley (fiber snow, silky snow, sun ray) tended to be further improved compared to the two-row barley (nishinohoshi).

[9. Evaluation of total amino acid content and total chlorophyll content of barley grown in Aso area and Tosu area]
The same as the above 7 except using the six-row barley fiber snow, silky snow, sunray and Ichibanboshi and Nijo hoshi nihonofoshi in the Aso and Tosu areas in the fall of 2014 for about 31 days after sowing and harvesting the seeds In addition, the total content of amino acids per 100 g of stem and leaf juice was measured by an automatic pre-column derivatization method using HPLC.

The total chlorophyll content of the stem and leaf juice was measured. A small amount of 800 μL of acetone and a small amount of calcium carbonate were added to 200 μL of the above-mentioned stem and leaf juice, mixed well, and allowed to stand in a dark room for 1 day. Centrifugation (15,000 rpm, 5 minutes) was performed, and the supernatant was used as a sample stock solution. A sample stock solution diluted with 80 vol% acetone was used as a sample solution, and 200 μL each was dispensed into a 96-well plate. Absorbances at 663 nm and 645 nm were measured at 750 nm with Varioscan. The amount of chlorophyll [mg / 100 g] was determined by the following formula.
Chlorophyll a [μg / mL] = 12.7 × (A 663 -A 750) -2.59 × (A 645 -A 750)
Chlorophyll b [μg / mL] = - 4.67 × (A 663 -A 750) + 22.9 × (A 645 -A 750)
Chlorophyll content [mg / 100 g] = (chlorophyll a + b) [μg / mL] × dilution factor × 0.1
A ₇₅₀ : absorbance at ₇₅₀ nm A ₆₆₃ : absorbance at ₆₆₃ nm A ₆₄₅ : absorbance at ₆₄₅ nm

About the measurement result of total amino acid amount and total chlorophyll amount, the relative value of Aso area cultivation barley on the basis of the measurement value of Aso area cultivation barley and a bird's-eye area cultivation barley and the measurement value of a bird's-eye area cultivation barley is shown in Table 17. As shown in Table 17, among the six-rowed barley grown in the Aso area, fiber snow, silky snow and sun ray affect the total amino acid content and color that affect the flavor compared with those grown in the bird area The total chlorophyll content was excellent. However, even with the same six-row barley, such a tendency was not found for Ichibanboshi. From these results, the Aso area has a better flavor than the Tosu area, and because it looks beautiful, it has a high cultivation suitability, and is rich in nutrition, and has a high palatability composition for eating and drinking green juice It has been found that suitable fiber snow, silky snow and shunrai can be grown.

According to the present invention, it is possible to provide a health food having a high content of nutrients derived from raw materials and umami ingredients. Further, according to the present invention, it is possible to provide a cultivation method capable of enhancing the taste and nutritional value while efficiently growing barley stems and / or leaves in a short period of time.

Claims (1)

A method according to the present invention for cultivating shunrai under conditions of daily difference of 10 ° C. or more on a monthly average.

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