JP2019004903A5 - - Google Patents
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- JP2019004903A5 JP2019004903A5 JP2018167419A JP2018167419A JP2019004903A5 JP 2019004903 A5 JP2019004903 A5 JP 2019004903A5 JP 2018167419 A JP2018167419 A JP 2018167419A JP 2018167419 A JP2018167419 A JP 2018167419A JP 2019004903 A5 JP2019004903 A5 JP 2019004903A5
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- 210000004027 cells Anatomy 0.000 claims 28
- 210000003716 Mesoderm Anatomy 0.000 claims 5
- 230000004156 Wnt signaling pathway Effects 0.000 claims 5
- 239000012190 activator Substances 0.000 claims 5
- 210000000130 stem cell Anatomy 0.000 claims 5
- 239000001963 growth media Substances 0.000 claims 4
- 239000000090 biomarker Substances 0.000 claims 3
- 239000003112 inhibitor Substances 0.000 claims 3
- 230000002401 inhibitory effect Effects 0.000 claims 3
- 210000000988 Bone and Bones Anatomy 0.000 claims 2
- 210000000845 Cartilage Anatomy 0.000 claims 2
- 210000002889 Endothelial Cells Anatomy 0.000 claims 2
- KWGKDLIKAYFUFQ-UHFFFAOYSA-M Lithium chloride Chemical group [Li+].[Cl-] KWGKDLIKAYFUFQ-UHFFFAOYSA-M 0.000 claims 2
- 210000002027 Muscle, Skeletal Anatomy 0.000 claims 2
- 210000001789 adipocyte Anatomy 0.000 claims 2
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims 2
- 230000002500 effect on skin Effects 0.000 claims 2
- 230000011664 signaling Effects 0.000 claims 2
- 239000000126 substance Substances 0.000 claims 2
- AQGNHMOJWBZFQQ-UHFFFAOYSA-N CT 99021 Chemical group CC1=CNC(C=2C(=NC(NCCNC=3N=CC(=CC=3)C#N)=NC=2)C=2C(=CC(Cl)=CC=2)Cl)=N1 AQGNHMOJWBZFQQ-UHFFFAOYSA-N 0.000 claims 1
- 210000001671 Embryonic Stem Cells Anatomy 0.000 claims 1
- 102000019058 Glycogen Synthase Kinase 3 beta Human genes 0.000 claims 1
- 108010051975 Glycogen Synthase Kinase 3 beta Proteins 0.000 claims 1
- 101700079341 MSGN1 Proteins 0.000 claims 1
- 102000004887 Transforming Growth Factor beta Human genes 0.000 claims 1
- 108090001012 Transforming Growth Factor beta Proteins 0.000 claims 1
- XHBVYDAKJHETMP-UHFFFAOYSA-N dorsomorphin Chemical compound C=1C=C(C2=CN3N=CC(=C3N=C2)C=2C=CN=CC=2)C=CC=1OCCN1CCCCC1 XHBVYDAKJHETMP-UHFFFAOYSA-N 0.000 claims 1
- 239000008194 pharmaceutical composition Substances 0.000 claims 1
Claims (16)
- 有効量のＷｎｔシグナル伝達経路の活性化剤を含む適切な培養培地中で多能性細胞を培養することにより得られうる人工沿軸中胚葉前駆細胞を含む、細胞集団。
- Ｗｎｔシグナル伝達経路の前記活性化剤がＧＳＫ−３βの阻害剤である、請求項１に記載の細胞集団。
- Ｗｎｔシグナル伝達経路の前記活性化剤がＲ−ｓｐｏｎｄｉｎである、請求項１に記載の細胞集団。
- Ｗｎｔシグナル伝達経路の前記活性化剤がＣＨＩＲ９９０２１である、請求項１に記載の細胞集団。
- Ｗｎｔシグナル伝達経路の前記活性化剤がＬｉＣｌである、請求項１に記載の細胞集団。
- 適切な前記培養培地がＤＭＳＯ又は等価物を更に含む、請求項１〜５のいずれか一項に記載の細胞集団。
- 適切な前記培養培地がＢＭＰシグナル伝達の化学的阻害剤を更に含む、請求項１〜６のいずれか一項に記載の細胞集団。
- ＢＭＰシグナル伝達の前記化学的阻害剤がＤｏｒｓｏｍｏｒｐｈｉｎである、請求項７に記載の細胞集団。
- 適切な前記培養培地がＴＧＦβ制御剤を含まない、請求項１〜８のいずれか一項に記載の細胞集団。
- 前記多能性細胞がヒト胚性幹細胞である、請求項１〜９のいずれか一項に記載の細胞集団。
- 前記多能性細胞がｉＰＳ細胞である、請求項１〜９のいずれか一項に記載の細胞集団。
- 前記細胞集団における細胞の少なくとも１０％が、高発現の沿軸中胚葉前駆細胞に特徴的なバイオマーカーを呈する請求項１〜１１のいずれか一項に記載の細胞集団。
- 前記集団における細胞の少なくとも３０％が、高発現の沿軸中胚葉前駆細胞に特徴的なバイオマーカーを呈する請求項１２に記載の細胞集団。
- 沿軸中胚葉前駆細胞に特徴的な前記バイオマーカーが、Ｍｓｇｎ１遺伝子産物である請求項１２又は１３に記載の細胞集団。
- 請求項１〜１４のいずれか一項に記載の細胞集団を含む、医薬組成物。
- 骨格筋系統、骨系統、軟骨系統、真皮細胞系統、脂肪細胞系統又は内皮細胞系統を含む細胞集団を調製する方法であって、
請求項１〜１４のいずれか一項に記載の細胞集団を提供する工程と、
骨格筋系統、骨系統、軟骨系統、真皮細胞系統、脂肪細胞系統又は内皮細胞系統を含む沿軸中胚葉誘導体のうちから選択される所望の細胞系統へと人工沿軸中胚葉前駆細胞を分化させるのに適切な条件下で、前記細胞集団を培養する工程とを含む、方法。
Applications Claiming Priority (8)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161528348P | 2011-08-29 | 2011-08-29 | |
| EP11306080A EP2565264A1 (en) | 2011-08-29 | 2011-08-29 | Method for preparing induced paraxial mesoderm progenitor (iPAM) cells and their use |
| US61/528,348 | 2011-08-29 | ||
| EP11306080.0 | 2011-08-29 | ||
| US201261654120P | 2012-06-01 | 2012-06-01 | |
| EP12305610 | 2012-06-01 | ||
| EP12305610.3 | 2012-06-01 | ||
| US61/654,120 | 2012-06-01 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014527647A Division JP6570833B2 (ja) | 2011-08-29 | 2012-08-29 | 人工沿軸中胚葉前駆（ｉＰＡＭ）細胞を調製する方法及びその使用 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2019004903A JP2019004903A (ja) | 2019-01-17 |
| JP2019004903A5 true JP2019004903A5 (ja) | 2019-03-28 |
Family
ID=50690361
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018167419A Pending JP2019004903A (ja) | 2011-08-29 | 2018-09-07 | 人工沿軸中胚葉前駆（ｉＰＡＭ）細胞を調製する方法及びその使用 |
Country Status (2)
| Country | Link |
|---|---|
| JP (1) | JP2019004903A (ja) |
| IL (1) | IL231205B (ja) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022094628A2 (en) * | 2020-11-02 | 2022-05-05 | Conception Biosciences, Inc. | In vitro derivation of gonadal somatic cells |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080038820A1 (en) * | 2004-06-22 | 2008-02-14 | Rudy-Reil Diane E | Induction of pluripotent stem cells into mesodermal lineages |
| KR101193541B1 (ko) * | 2009-08-24 | 2012-10-22 | 한국과학기술원 | 인간 배아줄기세포에서 중간엽 줄기세포로 분화시키는 방법 |
| JP6090868B2 (ja) * | 2011-08-19 | 2017-03-08 | エルジー・ケム・リミテッド | 偏光板 |
-
2014
- 2014-02-27 IL IL231205A patent/IL231205B/en active IP Right Grant
-
2018
- 2018-09-07 JP JP2018167419A patent/JP2019004903A/ja active Pending
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