JP2018530349A5 - - Google Patents
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- JP2018530349A5 JP2018530349A5 JP2018538060A JP2018538060A JP2018530349A5 JP 2018530349 A5 JP2018530349 A5 JP 2018530349A5 JP 2018538060 A JP2018538060 A JP 2018538060A JP 2018538060 A JP2018538060 A JP 2018538060A JP 2018530349 A5 JP2018530349 A5 JP 2018530349A5
- Authority
- JP
- Japan
- Prior art keywords
- human
- oocyte
- scnt
- cell
- embryo
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000010374 somatic cell nuclear transfer Methods 0.000 claims 65
- 210000000287 oocyte Anatomy 0.000 claims 62
- 239000013543 active substance Substances 0.000 claims 39
- 210000001161 mammalian embryo Anatomy 0.000 claims 33
- 210000001082 somatic cell Anatomy 0.000 claims 29
- 210000002257 embryonic structure Anatomy 0.000 claims 15
- 238000000034 method Methods 0.000 claims 15
- 230000000694 effects Effects 0.000 claims 13
- 210000004027 cell Anatomy 0.000 claims 12
- 108010074870 Histone Demethylases Proteins 0.000 claims 11
- 102000008157 Histone Demethylases Human genes 0.000 claims 11
- 210000002459 blastocyst Anatomy 0.000 claims 11
- 101000613625 Homo sapiens Lysine-specific demethylase 4A Proteins 0.000 claims 10
- 102100040863 Lysine-specific demethylase 4A Human genes 0.000 claims 10
- 230000004913 activation Effects 0.000 claims 10
- 230000011987 methylation Effects 0.000 claims 10
- 238000007069 methylation reaction Methods 0.000 claims 10
- 108060004795 Methyltransferase Proteins 0.000 claims 9
- 102000016397 Methyltransferase Human genes 0.000 claims 9
- 210000001671 embryonic stem cell Anatomy 0.000 claims 7
- 239000003112 inhibitor Substances 0.000 claims 7
- 150000007523 nucleic acids Chemical group 0.000 claims 7
- 108700040121 Protein Methyltransferases Proteins 0.000 claims 6
- 102000055027 Protein Methyltransferases Human genes 0.000 claims 6
- 230000003213 activating effect Effects 0.000 claims 6
- 101001088895 Homo sapiens Lysine-specific demethylase 4D Proteins 0.000 claims 5
- 102100033231 Lysine-specific demethylase 4D Human genes 0.000 claims 5
- 239000003795 chemical substances by application Substances 0.000 claims 5
- 239000012634 fragment Substances 0.000 claims 5
- 239000001963 growth medium Substances 0.000 claims 5
- 108020004707 nucleic acids Proteins 0.000 claims 5
- 102000039446 nucleic acids Human genes 0.000 claims 5
- 102000004169 proteins and genes Human genes 0.000 claims 5
- 108090000623 proteins and genes Proteins 0.000 claims 5
- 101000613629 Homo sapiens Lysine-specific demethylase 4B Proteins 0.000 claims 4
- 101001088893 Homo sapiens Lysine-specific demethylase 4C Proteins 0.000 claims 4
- 101001088900 Homo sapiens Lysine-specific demethylase 4E Proteins 0.000 claims 4
- 102100040860 Lysine-specific demethylase 4B Human genes 0.000 claims 4
- 102100033230 Lysine-specific demethylase 4C Human genes 0.000 claims 4
- 102100033232 Lysine-specific demethylase 4E Human genes 0.000 claims 4
- 108091030071 RNAI Proteins 0.000 claims 4
- 238000012258 culturing Methods 0.000 claims 4
- 230000009368 gene silencing by RNA Effects 0.000 claims 4
- 108091033409 CRISPR Proteins 0.000 claims 3
- 102100023696 Histone-lysine N-methyltransferase SETDB1 Human genes 0.000 claims 3
- 101000684609 Homo sapiens Histone-lysine N-methyltransferase SETDB1 Proteins 0.000 claims 3
- 238000009795 derivation Methods 0.000 claims 3
- 230000004927 fusion Effects 0.000 claims 3
- 238000002347 injection Methods 0.000 claims 3
- 239000007924 injection Substances 0.000 claims 3
- 230000000392 somatic effect Effects 0.000 claims 3
- 238000010354 CRISPR gene editing Methods 0.000 claims 2
- 108020005196 Mitochondrial DNA Proteins 0.000 claims 2
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 2
- 210000001109 blastomere Anatomy 0.000 claims 2
- 210000001771 cumulus cell Anatomy 0.000 claims 2
- 210000000805 cytoplasm Anatomy 0.000 claims 2
- 230000001605 fetal effect Effects 0.000 claims 2
- 210000002950 fibroblast Anatomy 0.000 claims 2
- 230000008014 freezing Effects 0.000 claims 2
- 238000007710 freezing Methods 0.000 claims 2
- 210000005260 human cell Anatomy 0.000 claims 2
- 238000000338 in vitro Methods 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 claims 2
- 239000002609 medium Substances 0.000 claims 2
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims 2
- 239000000203 mixture Substances 0.000 claims 2
- 108091023037 Aptamer Proteins 0.000 claims 1
- 241000282412 Homo Species 0.000 claims 1
- 108010021625 Immunoglobulin Fragments Proteins 0.000 claims 1
- 102000008394 Immunoglobulin Fragments Human genes 0.000 claims 1
- 108091034117 Oligonucleotide Proteins 0.000 claims 1
- 108091027967 Small hairpin RNA Proteins 0.000 claims 1
- 108020004459 Small interfering RNA Proteins 0.000 claims 1
- 210000001744 T-lymphocyte Anatomy 0.000 claims 1
- 102000004357 Transferases Human genes 0.000 claims 1
- 108090000992 Transferases Proteins 0.000 claims 1
- 210000003719 b-lymphocyte Anatomy 0.000 claims 1
- 210000001612 chondrocyte Anatomy 0.000 claims 1
- 238000005138 cryopreservation Methods 0.000 claims 1
- 238000002425 crystallisation Methods 0.000 claims 1
- 230000008025 crystallization Effects 0.000 claims 1
- 210000002308 embryonic cell Anatomy 0.000 claims 1
- 230000007159 enucleation Effects 0.000 claims 1
- 210000002919 epithelial cell Anatomy 0.000 claims 1
- 210000003743 erythrocyte Anatomy 0.000 claims 1
- 210000004602 germ cell Anatomy 0.000 claims 1
- 210000003958 hematopoietic stem cell Anatomy 0.000 claims 1
- 230000005764 inhibitory process Effects 0.000 claims 1
- 210000002510 keratinocyte Anatomy 0.000 claims 1
- 210000002540 macrophage Anatomy 0.000 claims 1
- 210000002752 melanocyte Anatomy 0.000 claims 1
- 210000001616 monocyte Anatomy 0.000 claims 1
- 210000000663 muscle cell Anatomy 0.000 claims 1
- 210000002569 neuron Anatomy 0.000 claims 1
- 230000003472 neutralizing effect Effects 0.000 claims 1
- 230000003169 placental effect Effects 0.000 claims 1
- 210000001778 pluripotent stem cell Anatomy 0.000 claims 1
- 108090000765 processed proteins & peptides Proteins 0.000 claims 1
- 229940121649 protein inhibitor Drugs 0.000 claims 1
- 239000012268 protein inhibitor Substances 0.000 claims 1
- 239000004055 small Interfering RNA Substances 0.000 claims 1
- 150000003384 small molecules Chemical class 0.000 claims 1
- 210000003014 totipotent stem cell Anatomy 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562239318P | 2015-10-09 | 2015-10-09 | |
| US62/239,318 | 2015-10-09 | ||
| US201562242050P | 2015-10-15 | 2015-10-15 | |
| US62/242,050 | 2015-10-15 | ||
| PCT/US2016/055890 WO2017062706A1 (en) | 2015-10-09 | 2016-10-07 | Methods and compositions to increase human somatic cell nuclear transfer (scnt) efficiency by removing histone h3-lysine trimethylation, and derivation of human nt-esc |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2018530349A JP2018530349A (ja) | 2018-10-18 |
| JP2018530349A5 true JP2018530349A5 (enExample) | 2019-11-14 |
Family
ID=58488507
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018538060A Pending JP2018530349A (ja) | 2015-10-09 | 2016-10-07 | ヒストンｈ３−リシントリメチル化の排除によりヒト体細胞核移入（ｓｃｎｔ）の効率を増加する方法および組成物、ならびにヒトｎｔ−ｅｓｃの誘導方法 |
Country Status (6)
| Country | Link |
|---|---|
| US (3) | US20180291400A1 (enExample) |
| EP (1) | EP3359167A4 (enExample) |
| JP (1) | JP2018530349A (enExample) |
| KR (1) | KR20210143952A (enExample) |
| CN (1) | CN109641015A (enExample) |
| WO (1) | WO2017062706A1 (enExample) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102473092B1 (ko) | 2014-09-15 | 2022-12-01 | 칠드런'즈 메디컬 센터 코포레이션 | 히스톤 h3-리신 트리메틸화를 제거함으로써 체세포 핵 이식(scnt) 효율을 증가시키는 방법 및 조성물 |
| CN107299113A (zh) * | 2017-06-12 | 2017-10-27 | 内蒙古大学 | H3K27me3及其去甲基化酶KDM6A/B在小鼠核移植重构胚中的应用方法 |
| CN108624621B (zh) * | 2018-01-17 | 2019-04-12 | 中国科学院上海生命科学研究院 | 非人灵长类的体细胞克隆动物的制备方法 |
| CN111630176A (zh) * | 2018-01-23 | 2020-09-04 | 车医科学大学校产学协力团 | 一种包括Rad51激活剂的用于胚胎发育的组合物以及用其提高胚胎发育率的方法 |
| WO2019147026A1 (ko) * | 2018-01-23 | 2019-08-01 | 차의과학대학교 산학협력단 | 멜라토닌을 포함하는, 배아 발달용 조성물 및 이를 이용하여 배아 발달의 효율을 향상시키는 방법 |
| WO2019195738A1 (en) * | 2018-04-06 | 2019-10-10 | Children's Medical Center Corporation | Compositions and methods for somatic cell reprogramming and modulating imprinting |
| EP3823663A4 (en) * | 2018-07-19 | 2022-03-09 | Children's Medical Center Corporation | COMPOSITIONS AND METHODS FOR GENERATING A PHYSIOLOGICAL X-CHROMOSOME INACTIVATION |
| EP4386081A3 (en) * | 2019-05-21 | 2024-10-16 | President and Fellows of Harvard College | Endocrine differentiation-inducing molecule |
| CN110607325B (zh) * | 2019-09-19 | 2021-03-23 | 广州医科大学附属第三医院（广州重症孕产妇救治中心、广州柔济医院） | 一种提高修正的人三原核受精卵体外培养囊胚发育率的方法 |
| CN116113421A (zh) | 2020-05-14 | 2023-05-12 | 阿莱兹精准医疗公司 | 使用siRNA调节PRDM2/RIZ蛋白表达的癌症治疗 |
| US20240350492A1 (en) * | 2020-07-10 | 2024-10-24 | Ting Therapeutics Llc | Methods for the Prevention and Treatment of Hearing Loss |
| US20230372311A1 (en) * | 2020-07-21 | 2023-11-23 | The Regents Of The University Of California | Compositions and methods of treating age-related retinal dysfunction |
| CN112382339B (zh) * | 2020-11-17 | 2024-08-13 | 中国人民解放军军事科学院军事医学研究院 | 一种识别合子型基因激活zga基因的方法及装置 |
| CN114805357B (zh) * | 2021-01-21 | 2023-12-19 | 四川大学 | 一种靶向setdb1-ttd的小分子抑制剂及其制药用途 |
| CN113186152B (zh) * | 2021-05-19 | 2023-05-26 | 南方医科大学 | Dna甲基转移酶抑制剂在提高动物圆形精子注射胚胎发育效率中的应用 |
| CN115247175B (zh) * | 2021-11-25 | 2025-10-17 | 南京启真基因工程有限公司 | 构建setdb1基因突变的表观遗传失调模型猪核移植供体细胞的基因编辑系统及其应用 |
| WO2025042589A1 (en) * | 2023-08-18 | 2025-02-27 | University Of Washington | Compositions and methods for improving cardiac regeneration |
| CN118956808B (zh) * | 2024-10-17 | 2025-01-07 | 天津赛萌生物技术有限公司 | 一种setdb1突变体及其在生物大分子制备中的应用 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1483817A (zh) * | 2002-09-18 | 2004-03-24 | 李建远 | 一种克隆无动物成分的人胚胎干细胞的方法 |
| CN101535468A (zh) * | 2005-10-21 | 2009-09-16 | 国际干细胞公司 | 单性生殖激活人类卵母细胞用于产生人类胚胎干细胞 |
| US20110172107A1 (en) * | 2008-04-30 | 2011-07-14 | Fox Chase Cancer Center | Assay for identifying agents that modulate epigenetic silencing, and agents identified thereby |
| WO2009143421A2 (en) * | 2008-05-22 | 2009-11-26 | The Johns Hopkins University | Methods for promoting fusion and reprogramming of somatic cells |
| US20130189780A1 (en) * | 2009-12-31 | 2013-07-25 | Fate Therapeutics, Inc. | Reprogramming compositions |
| CN105209606A (zh) * | 2013-02-15 | 2015-12-30 | 圣光医疗财团 | 使用体细胞核转移产生单性生殖干细胞和患者特异性人胚胎干细胞 |
| EP2997131A4 (en) * | 2013-05-13 | 2016-10-26 | Univ Oregon Health & Science | HUMAN PLURIPOTENTIAL STEM CELLS MADE BY SOMATIC CELL CORE TRANSFER |
| WO2014197835A2 (en) * | 2013-06-06 | 2014-12-11 | The General Hospital Corporation | Methods and compositions for the treatment of cancer |
-
2016
- 2016-10-07 US US15/765,860 patent/US20180291400A1/en not_active Abandoned
- 2016-10-07 JP JP2018538060A patent/JP2018530349A/ja active Pending
- 2016-10-07 KR KR1020187012570A patent/KR20210143952A/ko not_active Ceased
- 2016-10-07 EP EP16854382.5A patent/EP3359167A4/en not_active Withdrawn
- 2016-10-07 CN CN201680072631.1A patent/CN109641015A/zh active Pending
- 2016-10-07 WO PCT/US2016/055890 patent/WO2017062706A1/en not_active Ceased
-
2018
- 2018-04-09 US US15/948,781 patent/US20180298405A1/en not_active Abandoned
-
2019
- 2019-12-12 US US16/711,954 patent/US20200181648A1/en not_active Abandoned
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